Biological decontamination strategies using microorganisms to adsorb aflatoxins have shown promising results for reducing the dietary exposure to these contaminants. In this study, the ability of inactivated biomasses of Lacticaseibacillus rhamnosus (LRB) and Saccharomyces cerevisiae (SCB) incorporated alone or in combination into functional yogurts (FY) at 0.5–4.0% (w/w) to adsorb aflatoxin B₁ (AFB₁) was evaluated in vitro. Higher adsorption percentages (86.9–91.2%) were observed in FY containing 1.0% LR + SC or 2.0% SC (w/w). The survival of mouse embryonic fibroblasts increased after exposure to yogurts containing LC + SC at 1.0–4.0% (w/w). No significant differences were noted in the physicochemical and sensory characteristics between aflatoxin-free FY and control yogurts (no biomass) after 30 days of storage. The incorporation of combined LRB and SCB into yogurts as vehicles for these inactivated biomasses is a promising alternative for reducing the exposure to dietary AFB₁. The results of this trial support further studies to develop practical applications aiming at the scalability of using the biomasses evaluated in functional foods to mitigate aflatoxin exposure.

Contamination by multi-mycotoxins in cattle feed can lead to increased susceptibility to diseases and loss of performance. The present study aimed to investigate the occurrence of multiple mycotoxins present in the diet of beef cattle feedlots in Brazil. Chromatographic analyses were performed on 152 TMR samples from seven states, representing the diet provided to 1,246,522 animals. Contamination by mycotoxins was found in 100% of the TMR samples analyzed, with the most frequent being fumonisins, present in 100% of the samples, followed by zearalenone, which contaminated 79.6% of the samples, and subsequently by aflatoxins, deoxynivalenol, and T-2, while HT-2 was not detected in any of the samples. Furthermore, 2.6% of samples showed co-occurrence of five different types of mycotoxins, 23.7% presented four mycotoxins, 41.4% three mycotoxins, 22.4% two mycotoxins, and 9.9% of the samples showed contamination by only one mycotoxin. The significant prevalence of mycotoxins of the Fusarium and Aspergillus genera in the samples of the present study indicates a notable degree of pre- and post-harvest contamination in these beef cattle diets. Further studies are needed to define methods for monitoring cattle exposure to clarify its effects, even at low levels, and reduce the impacts on beef cattle production in Brazil.

The diet of dairy calves can be contaminated with mycotoxins, posing a potential risk to animal health. This case study report aimed to make the first assessment of the presence of multiple mycotoxins in concentrates fed to dairy calves in Brazil. A total of 19 concentrate samples intended for dairy calves were analyzed using liquid chromatography coupled with mass spectrometry. Aflatoxins, deoxynivalenol, and T-2 toxin were not detected in any samples, whereas fumonisins B₁ (FB₁) and B₂ (FB₂) were present in 100% of the samples, with mean concentrations of 2750.1 μg/kg and 834.9 μg/kg, respectively. Zearalenone (ZEN) was detected in 36.8% of samples, with a mean concentration of 929.9 μg/kg. Significant correlations were observed between FB₁ and FB₂ (ρ = 0.978; p < 0.001) and between FB₂ and ZEN (ρ = 0.735; p = 0.05). While the physical form of the concentrate did not influence (p > 0.05) mycotoxin concentrations, a trend was observed for FB₁ (ρ = −0.417; p = 0.07) and FB2 (ρ = −0.395; p = 0.09). These findings highlight the frequent occurrence of Fusarium mycotoxins, likely due to pre-harvest contamination, emphasizing the potential risk of additive or synergistic effects in dairy calves.

The utilization of strains of probiotic microorganisms has been demonstrated to be effective in the removal of mycotoxins. This study aimed to evaluate the in vitro adsorption capacity of aflatoxin B1, by Lacticaseibacillus rhamnosus and Saccharomyces cerevisiae, either alone and in combination, as well as the stability of the adsorbent/mycotoxin complex in each of the situations. Aflatoxin B1 working solutions for the assays were prepared in potassium phosphate buffer at pH 3.0 and pH 6.5, using aliquots of lactic acid bacteria and yeast culture containing a biomass of inactivated cells, either alone or in combination. After incubation, the solution was centrifuged, and the supernatant was separated for aflatoxin B1 quantification. The stability test of the aflatoxin B1 complex formed with all the mentioned treatments was carried out using the pellets obtained after centrifugation of the binding assays, performing multiple washes with phosphate buffer. The supernatant from each wash was analyzed for the amount of aflatoxin B1 released. The best aflatoxin B1 adsorption rate occurred with the use of lactic acid bacteria/S. cerevisae at both pH 3.0 (61.9%) and 6.5 (68.1%), followed by S. cerevisiae isolated at pH 3.0 (57.4%). Regarding the stability of the adsorbent/mycotoxin complex, they showed greater stability to S. cerevisae isolated at pH 3.0 (85.7%) and less stability to lactic acid bacteria at pH 3.0 (56.2%).