The rise of autonomous vehicles necessitates advanced communication networks for effective data exchange. The routing protocols Ad hoc On-Demand Distance Vector (AODV) and Greedy Perimeter Stateless Routing (GPSR) are vital in mobile networks (MANETs) and vehicular ad hoc networks (VANETs). However, their performance is affected by changing network conditions. This study examines key routing parameters—MaxJitter, Hello/Beacon Interval, and route validity time—and their impact on AODV and GPSR performance in urban and highway scenarios. The simulation results reveal that increasing MaxJitter enhances AODV throughput by 12% in cities but decreases it by 8% on highways, while GPSR throughput declines by 15% in cities and 10% on highways. Longer Hello intervals improve AODV performance by 10% in urban settings but reduce it by 6% on highways. Extending route validity time increases GPSR’s Packet Delivery Ratio (PDR) by 10% in cities, underscoring the need to optimize routing parameters for enhanced VANET performance.

Escherichia coli O157:H7 is a strain of Escherichia coli known for causing foodborne illness through the consumption of contaminated or raw food. To detect this pathogen, a conductometric immunosensor was developed using a conductometric sensing approach. The sensor was constructed on an interdigitated electrode and modified with a monoclonal anti-Escherichia coli O157:H7 aptamer. A total of 200 electrode pairs were fabricated and modified to bind to the target molecule replica. The binding replica, acting as the bio-recognizer, was linked to the electrode surface using 3-Aminopropyl triethoxysilane. The sensor exhibited excellent performance, detecting Escherichia coli O157:H7 in a short time frame and demonstrating a wide detection range of 1 fM to 1 nM. Concentrations of Escherichia coli O157:H7 were detected within this range, with a minimum detection limit of 1 fM. This innovative sensor offers simplicity, speed, high sensitivity, selectivity, and the potential for rapid sample processing. The potential of this proposed biosensor is particularly beneficial in applications such as drug screening, environmental monitoring, and disease diagnosis, where real-time information on biomolecular interactions is crucial for timely decision-making and where cross-reactivity or interference may compromise the accuracy of the analysis.

Despite advancements in oncology, predicting recurrence-free survival (RFS) in head and neck (H&N) cancer remains challenging due to the heterogeneity of tumor biology and treatment responses. This study aims to address the research gap in the prognostic efficacy of traditional clinical predictors versus advanced radiomics features and to explore the potential of weighted fusion techniques for enhancing RFS prediction. We utilized clinical data, radiomic features from CT and PET scans, and various weighted fusion algorithms to stratify patients into low- and high-risk groups for RFS. The predictive performance of each model was evaluated using Kaplan–Meier survival analysis, and the significance of differences in RFS rates was assessed using confidence interval (CI) tests. The weighted fusion model with a 90% emphasis on PET features significantly outperformed individual modalities, yielding the highest C-index. Additionally, the incorporation of contextual information by varying peritumoral radii did not substantially improve prediction accuracy. While the clinical model and the radiomics model, individually, did not achieve statistical significance in survival differentiation, the combined feature set showed improved performance. The integration of radiomic features with clinical data through weighted fusion algorithms enhances the predictive accuracy of RFS outcomes in head and neck cancer. Our findings suggest that the utilization of multi-modal data helps in developing more reliable predictive models and underscore the potential of PET imaging in refining prognostic assessments. This study propels the discussion forward, indicating a pivotal step toward the adoption of precision medicine in cancer care.

Vehicular Communication Networks (VCNs) are essential to intelligent transportation systems, where real-time data exchange between vehicles and infrastructure supports safety, efficiency, and automation. However, achieving high Quality of Service (QoS)—especially under delay-sensitive conditions—remains a major challenge due to the high mobility and dynamic topology of vehicular environments. While some efforts have explored routing protocol optimization, few have systematically compared multiple optimization approaches tailored to distinct traffic and delay conditions. This study addresses this gap by evaluating and enhancing two widely used routing protocols, QOS-AODV and GPSR, through their improved versions, CM-QOS-AODV and CM-GPSR. Two distinct optimization models are proposed: the Traffic-Oriented Model (TOM), designed to handle variable and high-traffic conditions, and the Delay-Efficient Model (DEM), focused on reducing latency for time-critical scenarios. Performance was evaluated using key QoS metrics: throughput (rate of successful data delivery), packet delivery ratio (PDR) (percentage of successfully delivered packets), and end-to-end delay (latency between sender and receiver). Simulation results reveal that TOM-optimized protocols achieve up to 10% higher PDR, maintain throughput above 0.40 Mbps, and reduce delay to as low as 0.01 s, making them suitable for applications such as collision avoidance and emergency alerts. DEM-based variants offer balanced, moderate improvements, making them better suited for general-purpose VCN applications. These findings underscore the importance of traffic- and delay-aware protocol design in developing robust, QoS-compliant vehicular communication systems.

Vehicular Ad Hoc Networks play a crucial role in enabling Smart City applications by facilitating seamless communication between vehicles and infrastructure. This study evaluates the throughput performance of different routing protocols, specifically AODV, AODV:TOM, AODV:DEM, GPSR, GPSR:TOM, and GPSR:DEM, under various city and highway scenarios in complex networks. The analysis covers key parameters including traffic generation, packet sizes, mobility speeds, and pause times. Results indicate that TOM and DEM profiles significantly improve throughput compared to traditional AODV and GPSR protocols. GPSR:TOM achieves the highest throughput across most scenarios, making it a promising solution for high-performance data transmission in Smart Cities. For instance, GPSR:TOM achieves an average throughput of 3.2 Mbps in city scenarios compared to 2.8 Mbps for GPSR, while in highway scenarios, the throughput increases to 3.6 Mbps. Additionally, AODV:DEM records a throughput of 3.4 Mbps for high traffic generation, outperforming AODV:TOM at 3.1 Mbps and baseline AODV at 2.7 Mbps. The findings highlight the importance of optimizing data throughput to ensure reliability and efficiency in complex vehicle interconnection systems, which are critical for traffic management, accident prevention, and real-time communication in smart urban environments.