The life-cycle cost analysis is a method used to assess long-term economic efficiency among equivalent competing processes or products. The purpose of this paper is to investigate the nature and level of costs for an organic orchard located in Southern Romania, using a complex approach covering the entire chain of production, through its life span. The research results, based on a dynamic analysis and an integrated evaluation of the orchard’s performance, were ranked on investment and operational costs and broken down into three categories (establishment, production, post-harvest, transport costs). The highest costs, representing 151,726 EUR/ha/20 years, about 52.72% of the total operational costs and 50.4% of the total farm costs/ha/20 years, were recorded in the exploitation stage. The scenarios for the sensitivity analysis considered different levels of average yields (40 and 60 tons/ha, respectively) with different rates of sold productions (85%, optimistic scenario; 70%, pessimistic scenario). The hot points identified at the production stage were the use of agricultural machinery, several pesticides, the costs of seedlings, anti-hail nets, plastic boxes, and labor costs, while at the post-harvest stage, there were those related to labor and energy consumption. The transport stage had important costs with respect to tractor operations and the track.

The circular economy with compost fertilization is included in the sustainable orchard paradigm, creating a holistic production ecosystem. Modern orchards are mostly intensive and super-intensive, requiring different rootstocks. This research presents the response to compost fertilization of two specific pear rootstocks (quince ‘CTS 212’ and ‘Farold^® 40’) and own-rooted trees, analyzing six resistant cultivars in a circular production system. The dynamic of nitrogen and carbon concentration in leaves, soil respiration coefficient, the evolution of the fruit maturity stage in the field, and some biometric parameters such as trunk cross-section area, the annual vegetative growth, and fruiting shoots annual number were analyzed. The results highlighted that compost fertilization led to increased leaf nitrogen concentration over the first two years while carbon concentration remained relatively stable. Rootstock and compost fertilization influenced the fruit maturity dynamic, but a single pattern was not identified. Quince, as pear rootstock, expressed a higher sensitivity to compost application; the biometric parameters, such as trunk cross-section area, and almost all cultivars’ annual vegetative growth were higher than the controls’. Positive output can lead to future model upscaling in farms and households.

Life Cycle Cost Analysis is a method used to assess long-term economic efficiency among equivalent competing processes or products. The purpose of the paper is to investigate the nature and level of costs for an organic orchard located in Southern Romania, using a complex approach covering the entire chain of production, through the life span. The research results, based on a dynamic analysis and an integrated evaluation of the orchard's performance, have been ranked on investment and operational costs, broken down on three categories (establishment and production, post-harvest and transport costs). The highest cost, representing 151726 euros/ha/20 years, about 52.72% of the total operational costs and 50.4% of the total farm costs/ha/20 years, was recorded in exploitation stage. The scenarios for the sensitivity analysis considered different levels of average yield (40, and, respectively 60 tons/ha) with different rate of sold production (85%, optimistic scenario; 70%, pessimistic scenario). The hotpoints identified at the production stage were use of agricultural machinery, several pesticides, the costs of seedlings, anti-hail net, plastic boxes and labor cost, while at the post-harvest stage, were those related to labor and energy consumption. The transport stage has had important costs with tractor operation and the track.

Since its domestication about a century ago in North America, highbush blueberry (Vaccinium corymbosum L.) has gained appreciation by consumers worldwide, and the demand for new blueberry varieties is increasing. Whole-genome resequencing can help plant breeders to decrease the time needed to create novel varieties by identifying novel genes linked to fruit-quality traits. The present study analyzed the genetic variability of eight V. corymbosum genotypes, seven Romanian varieties (‘Prod’, ‘Vital’, ‘Azur’, ‘Simultan’, ‘Delicia’, ‘Compact’, and ‘Safir’), and the American variety, ‘Bluecrop’. The analysis of the first ~10 Mb from scaffold 22, a hotspot of genomic variation, in the above-mentioned varieties revealed multiple differences in 11 upregulated and 50 downregulated genes involved in fruit growth and development. Of these differentially regulated genes, two upregulated and five downregulated genes were fully covered by at least 1× coverage depth by sequencing. The genes’ sequence analysis confirmed the high genetic variability of the region, with most of the genes presenting numerous SNPs and some InDels, and indicated that an attempted 10× medium-coverage depth of sequencing for V. corymbosum varieties yields useful preliminary data for use in breeding programs.

This exploratory study examined fruit production, dry matter content, mineral composition, and estimated nutrient removal in three nectarine (Prunus persica var. nucipersica) cultivars during the 2025 growing season in a commercial intensive orchard in northwestern Romania. ‘Big Top’, ‘Venus’, and ‘Cora’ grafted onto GF 677 rootstock were tested under uniform orchard management circumstances, which included fertigation with a water-soluble NPK 15-15-15 fertilizer. ‘Big Top’ had the largest fruit yield (34.82 t ha⁻¹) and average fruit weight, whereas ‘Cora’ had the highest dry matter content and mean concentrations of N, P, K, Ca, and Mg in the studied fruit tissues. ‘Venus’ generally displayed intermediate values. ‘Cora’ also had the highest estimated nutrient removal per ton and hectare of harvested fruit, while ‘Big Top’ had the lowest values. Potassium was the biggest component of estimated nutrient removal across all three cultivars. These descriptive patterns demonstrate the need to take into account fruit mineral composition as well as yield when evaluating nutrient removal. However, the mineral analyzes did not include fruit stones; therefore, the values do not represent total whole-fruit nutrient export. Given the study’s single-year, single-site design, the findings should be viewed as preliminary, as they do not establish statistically significant cultivar effects or warrant cultivar-specific fertilizer recommendations. To examine the consistency and practical applicability of these findings, additional multi-year investigations using thorough fruit analyzes and adequate statistical evaluations are required.

The circular economy with compost fertilization is included in the sustainable orchard paradigm, creating a holistic production ecosystem. Modern orchards are mostly intensive and super-intensive, requiring different rootstocks. This research presents the response to compost fertilization of two specific pear rootstocks (quince ‘CTS 212’ and ‘Farold® 40’) and own-rooted trees, analyzing six resistant cultivars in a circular production system. The dynamic of nitrogen and carbon content in leaves, soil respiration coefficient, the evolution of the fruit maturity stage in the field, and some biometric parameters such as trunk cross-section area, the annual vegetative growth, and fruiting shoots annual number were analyzed. The results highlighted the effect of compost fertilization on plants: almost all variants presented higher nitrogen amounts in leaves in the first and second years. Carbon content in leaves had a lower dynamic over time. Rootstock and compost fertilization influenced the fruit maturity dynamic, but a single pattern was not identified. Quince as pear rootstock expressed a higher sensitivity to compost application, the biometric parameters as trunk cross-section area, and almost all cultivars' annual vegetative growth were higher than the control's. Positive output can lead to future model upscaling in the farms and households.