The aim of the study was to develop composite film materials derived from modified sodium carboxymethyl cellulose and to evaluate their anti-adhesive effects. Materials and Methods. The modified film materials were obtained by dissolving sodium carboxymethyl cellulose (Na- CMC) in an aqueous solution of a modifier (glycoluril) with subsequent homogenization and drying in a vacuum drying oven at room temperature. Physicomechanical parameters of the obtained films were determined using the Instron 3369 universal electromechanical testing machine (Great Britain) equipped with a climatic chamber (300-523 K), improved video extensometer, and the MKC-25 micrometer (Russia). Cytotoxicity of glycoluril-modified film materials derived from Na-CMC was studied by incubating cell cultures of 3T3-L1 mouse fibroblasts directly with extracts from films under study using a colorimetric test. Their anti-adhesion effect was investigated on 40 female Wistar rats by modeling a flat adhesion by inducing abrasion of the cecum and suturing the deserosed surface of the abdominal wall anatomically opposite the abrasion area. The presence of adhesions was assessed on day 8 after the operation. Commercial membrane Seprafilm (USA) was used as a reference sample. Results. It was found that extracts obtained from film materials derived from Na- CMC modified with glycoluril at a concentration of 0.01 and 0.05 wt. % had no cytotoxic effect on the cell culture of mouse fibroblasts 3T3-L1. Flat adhesions were not detected when using Seprafilm. When film materials under study were placed in the abdominal cavity between the injured areas, formation of flat adhesions was not observed or observed in one case out of ten. Conclusion. The obtained films are promising for preventing adhesions as a barrier-type agent. Modifying Na-CMC with glycoluril made it possible to create films that prevent formation of flat adhesions, have improved physical and mechanical properties and no cytotoxic effect.

Due to traumatic injuries, including those from surgical procedures, adhesions occur in over 50% of cases, necessitating exclusive surgical intervention for treatment. However, preventive measures can be implemented during abdominal organ surgeries. These measures involve creating a barrier around internal organs to forestall adhesion formation in the postoperative phase. Yet, the effectiveness of the artificial barrier relies on considerations of its biocompatibility and the avoidance of adverse effects on the body. This study explores the biocompatibility aspects, encompassing hemocompatibility, cytotoxicity, and antibacterial and antioxidant activities, as well as the adhesion of blood serum proteins and macrophages to the surface of new composite film materials. The materials, derived from the sodium salt of carboxymethylcellulose modified by glycoluril and allantoin, were investigated. The research reveals that film materials with a heterocyclic fragment exhibit biocompatibility comparable to commercially used samples in surgery. Notably, film samples developed with glycoluril outperform the effects of commercial samples in certain aspects.

Purpose of the study: to conduct a comparative assessment of the formation of the sphincter mechanism during colostomy using titanium nickelide-based implants in an experiment.Material and methods. The experimental study was conducted on 45 sexually mature male rats Wistar with an average body weight of (531.3 ± 13.4) g. The animals were randomized into three equal groups of 15 animals each. In the 1st experimental group, a cylindrical spiral made of titanium nickelide with invagination of the serosalmuscular layer of the colon was used to create a colostomy. In the 2nd experimental group, a similar spiral was used, but without invagination of the serous-muscular layer. The control group underwent the standard procedure of single-barrel colostomy formation. Animals were removed from the experiment on the 7th, 30th and 60th days after the operation, 5 animals at each stage. Histologic examination of the area of the formed colostomy with constipation apparatus was performed.Results. The use of cylindrical spiral implants from titanium nickelide in colostomy formation (both with and without invagination of the serosal-muscular layer of the colon) had no significant effect on the dynamics of body weight of experimental animals compared to the classical method. Colostomy formation without invagination of the serous-muscular layer did not lead to an increase in the incidence of postoperative complications in comparison with the traditional method. However, invagination of the serous-muscular layer was accompanied by an increased incidence of complications in the postoperative period.Conclusion. The complex analysis of experimental data, including the assessment of survival rate, body weight dynamics, incidence of postoperative complications, morphological changes, the course of regenerative processes and stoma functionality, allows us to recommend as a promising method of colostomy the use of a cylindrical spiral made of titanium nickelide without invagination of the serous-muscular layer of the intestine.