Mucilage extracted from the parenchymatous and chlorenchymatous tissues of Opuntia robusta were obtained using water or ethanol as the extraction solvent. The changes in the different tissues by using different extraction solvents were evaluated via scanning electron microscopy (SEM) and Fourier transform infrared (FT-IR) and Raman spectroscopy; in addition, the effect of mucilage coating on the various quality characteristics of tomato (Lycopersicum sculentum) was evaluated. The SEM results showed that the mucilage extracted from the parenchyma had a higher aggregation level that the mucilage extracted from the chlorenchyma. The presence of three characteristic bands of pectic substances in the FT-IR spectra between 1050 and 1120 cm⁻¹ indicated that the mucilage extracted from the parenchymatous tissue had a higher content of pectic compounds than the mucilage extracted from the chlorenchymatous tissue. It was also observed in the Raman spectra that the level of pectic substances in the mucilage extracted from the parenchymatous was higher than that in the mucilage extracted from the chlorenchymatous tissue. The mucilage extracted from the parenchymatous tissue was more effective as an edible coating than the mucilage extracted from the chlorenchymatous tissue. Tomatoes covered with mucilage showed significantly enhanced firmness and reduced weight loss. The uncoated tomatoes showed higher lycopene content than the coated tomatoes on the 21st day. This study showed that the Opuntia robusta tissue and extraction solvent influence mucilage characteristics and that Opuntia robusta mucilage is a promising edible coating.

The inflorescences of Chamaedorea tepejilote Liebm. are consumed as food in Central America and southern Mexico but is an underutilized food because of its sensory characteristics, principally due to its bitter taste. However, the inflorescences of Chamaedorea tepejilote Liebm. are nutritionally promising due to their high protein content (approximately 25%). Protein isolates from pacaya were modified via three different thermal treatments to determine the effect of the treatments on the protein structures. Scanning electron microscopy indicated that the pacaya protein isolate particles had less rough and irregular surfaces with larger particle sizes due to an aggregation process when a thermal treatment was used compared to those when no thermal treatment was used. An increase in the intensity of the low molecular weight protein fractions (≤20 kDa) in the electrophoretic pattern of the proteins was observed, which was generated by the hydrolysis of the proteins by heat treatment. The modifications in the FT-IR spectra showed that thermal treatment of pacaya affected the secondary structure of its proteins, mainly when microwave treatment was used. Raman spectroscopy revealed that the α–helical structure was dominant in the proteins of pacaya and that thermal treatment increased the fraction of the β–sheet structure at the expense of the α–helical structure.

In this study, a partial purification and characterization of the lectins from two varieties of Phaseolus coccineus (black and purple ayocote bean) was carried out. A partial purification of the lectins was performed by affinity chromatography on fetuin-agarose. The lectins’ characterization showed that the hemagglutination activity was only inhibited by the fetuin and that the saccharides (mono-, di- and tri-saccharides) do not influence their hemagglutination capacity. A difference in the relative molecular weight between the two Phaseolus varieties was observed, 28.5 kDa for the black variety and 30.6 kDa for the purple variety. In the lectins of both Phaseolus varieties, ions were detected in their structure and influenced their hemagglutination activity, principally to the ion Ca⁺²; on the other hand, only the lectins of the purple variety of the Phaseolus, had a detectable ion, Cu⁺². The lectins showed thermostability in the temperature range of 25 and 70 °C, on the other hand, in the pH range between 3 and 10, the lectins showed hemagglutination activity. According to the obtained results, the structural differences between the lectins obtained from both Phaseolus varieties influence their hemagglutination activity.

Nopal (Opuntia) is a resource that is discarded after pruning complete cladodes. For this reason, the aim of this study was to investigate the effects of the combined use of organic matter (nopal) and goat manure on changes in soil characteristics and on the growth and yield performance of carrot and tomato plants. Physicochemical analysis of the soil and goat manure was carried out, and germination tests and physical characterization of the plants and the products obtained were performed on tomato and carrot plants after adding the components of the cladodes alone and the cladodes with goat manure. The results showed that the application of the nopal cladode components increased the cation exchange capacity of the soil. The highest germination rate was obtained by the application of liquid nopal, with a 1.7-fold increase in the germination rate of carrot seeds, while that for tomato seeds was only 14%. Similar results were obtained for the germination percentage. An increase in resistance to water stress of approximately 15 days was observed in both tomato and carrot plants. Plant development and production were achieved in tomato, with a 100% increase in carrot plant length and a threefold increase in production. The addition of nopal to the soil improved the soil characteristics and the production yield of carrots and tomatoes.

Three biological activities were assessed for peptides obtained from two ayocote bean varieties, antioxidant and antihypertensive properties and antiproliferative activity against MDA breast cancer cells. For the peptides obtained from the ayocote bean black variety the highest antihypertensive activity was obtained with low-molecular weight peptides, principally with those between 5 and 10 kDa. However, for the peptides obtained from ayocote bean purple variety, the highest antihypertensive activity was achieved with peptides with a molecular weight ≥10 kDa, while that the higher antioxidant activity was noted for peptides obtained from ayocote bean purple variety. All peptides from both purple and black ayocote beans varieties inhibited MDA cells proliferation; however, the IC₅₀ was only reached with high concentrations of peptides (2000 or 5000 µg*mL −1) and exposure times of 12 or 72 h. The obtained results reveal a potential relationship among the antihypertensive, antioxidant activities and anticancer properties of the peptides.