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Dr. Sabato D’Auria
Consiglio Nazionale delle Ricerche, Institute of Food Science, Via Roma 64, Avellino, Italy

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0 Biosensors
0 Enzymology
0 Protein Structure
0 Protein biochemistry
0 Fluorescence Protein

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Protein Structure
Fluorescence Protein
Protein biochemistry

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Journal article
Published: 09 August 2021 in Microorganisms
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We evaluated the polyphenol content and the α-glucosidase activity exhibited by different monofloral honeys of Italian origin. Their capacity to act on different pathogenic (Acinetobacter baumannii, Escherichia coli, Listeria monocytogenes, Pseudomonas aeruginosa, and Staphylococcus aureus) as well as probiotic bacteria (Lacticaseibacillus casei, Lactobacillusacidophilus, Lactiplantibacillus plantarum, Lactobacillus gasseri, and Lacticaseibacillus rhamnosus) was also assessed. Total polyphenols varied between 110.46 μg/g of fresh product (rhododendron honey) and 552.29 μg/g of fresh product (strawberry tree honey). Such result did not correspond to a parallel inhibitory α-glycosidase activity that, in each case was never higher than 33 μg/mL. Honeys were differently capable to fight the biofilm formation of the pathogens (inhibition up to 93.27%); they inhibited the in vitro adhesive process (inhibition up to 84.27%), and acted on mature biofilm (with values up to 76.64%). Their effect on bacterial metabolism was different too. Honeys were ineffective to inhibit E. coli mature biofilm nor to act on its metabolism. The action of the honey on probiotic strains seemed almost always stimulate their growth. Thus, these monofloral honeys might exhibit effects on human health and act positively as prebiotics.

ACS Style

Florinda Fratianni; Maria Ombra; Antonio D’Acierno; Lucia Caputo; Giuseppe Amato; Vincenzo De Feo; Raffaele Coppola; Filomena Nazzaro. Polyphenols Content and In Vitro α-Glycosidase Activity of Different Italian Monofloral Honeys, and Their Effect on Selected Pathogenic and Probiotic Bacteria. Microorganisms 2021, 9, 1694 .

AMA Style

Florinda Fratianni, Maria Ombra, Antonio D’Acierno, Lucia Caputo, Giuseppe Amato, Vincenzo De Feo, Raffaele Coppola, Filomena Nazzaro. Polyphenols Content and In Vitro α-Glycosidase Activity of Different Italian Monofloral Honeys, and Their Effect on Selected Pathogenic and Probiotic Bacteria. Microorganisms. 2021; 9 (8):1694.

Chicago/Turabian Style

Florinda Fratianni; Maria Ombra; Antonio D’Acierno; Lucia Caputo; Giuseppe Amato; Vincenzo De Feo; Raffaele Coppola; Filomena Nazzaro. 2021. "Polyphenols Content and In Vitro α-Glycosidase Activity of Different Italian Monofloral Honeys, and Their Effect on Selected Pathogenic and Probiotic Bacteria." Microorganisms 9, no. 8: 1694.

Journal article
Published: 07 July 2021 in Life
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Knowledge of the composition of the gut microbiota in freshwater fish living in their natural habitat has taxonomic and ecological importance. Few reports have been produced on the composition of the gut microbiota and on the presence of LAB in the intestines of freshwater fish that inhabit river environments. In this study, we investigated the LAB community that was present in the gastrointestinal tract (GIT) of Mediterranean trout (Salmo macrostigma) that colonized the Biferno and Volturno rivers of the Molise region (Italy). The partial 16S rRNA gene sequences of these strains were determined for the species-level taxonomic placement. The phylogenetic analysis revealed that the isolated LABs belonged to seven genera (Carnobacterium, Enterococcus, Lactobacillus, Lactiplantibacillus, Vagococcus, Lactococcus, and Weissella). The study of the enzymatic activities showed that these LABs could contribute to the breakdown of polysaccharides, proteins, and lipids. In future studies, a greater understanding of how the LABs act against pathogens and trigger the fish immune response may provide practical means to engineer the indigenous fish microbiome and enhance disease control and fish health.

ACS Style

Massimo Iorizzo; Gianluca Albanese; Bruno Testa; Mario Ianiro; Francesco Letizia; Mariantonietta Succi; Patrizio Tremonte; Mariasilvia D’Andrea; Nicolaia Iaffaldano; Raffaele Coppola. Presence of Lactic Acid Bacteria in the Intestinal Tract of the Mediterranean Trout (Salmo macrostigma) in Its Natural Environment. Life 2021, 11, 667 .

AMA Style

Massimo Iorizzo, Gianluca Albanese, Bruno Testa, Mario Ianiro, Francesco Letizia, Mariantonietta Succi, Patrizio Tremonte, Mariasilvia D’Andrea, Nicolaia Iaffaldano, Raffaele Coppola. Presence of Lactic Acid Bacteria in the Intestinal Tract of the Mediterranean Trout (Salmo macrostigma) in Its Natural Environment. Life. 2021; 11 (7):667.

Chicago/Turabian Style

Massimo Iorizzo; Gianluca Albanese; Bruno Testa; Mario Ianiro; Francesco Letizia; Mariantonietta Succi; Patrizio Tremonte; Mariasilvia D’Andrea; Nicolaia Iaffaldano; Raffaele Coppola. 2021. "Presence of Lactic Acid Bacteria in the Intestinal Tract of the Mediterranean Trout (Salmo macrostigma) in Its Natural Environment." Life 11, no. 7: 667.

Journal article
Published: 02 July 2021 in Foods
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The use of natural compounds as food preservatives is becoming increasingly popular as it is perceived positively by consumers. Among these substances, essential oils have attracted great interest owing to their antioxidant and antimicrobial properties. However, several challenges impair the use of essential oils in food products, such as their degradation or loss during food processing and storage, the strong aroma, even at low concentrations, which may negatively affect the sensory characteristics of food. In this context, the development of nanoformulations able to stabilize essential oils may represent a smart solution to this issue. The aim of the study was to evaluate the efficiency of alginate-based nanoformulations enriched with lemongrass (Cymbopogon nardus) essential oil (LEO) and Tween 80 against several fungi namely Penicillium expansus, Aspergillus niger and Rhizopus spp. Firstly, the flow behavior of systems at different concentrations of alginate (1%, 2% and 3% w/w) were studied. Then, emulsion-based nanoformulations at different concentrations of lemongrass essential oil in the range of 0–2% w/w were stabilized by a fixed amount of Tween 80, characterized and tested for their antifungal activity. Our results showed that the best nanoformulation able to inhibit Rhizopus spp., Penicillium expansum and Aspergillus niger, for at least 10 days, was constituted by 1% alginate/1.5% LEO/1% Tween 80. Hence, the incorporation of essential oil into nanoformulation systems may represent a valid alternative to overcome the disadvantages that limit the commercial application of essential oils.

ACS Style

Martina Cofelice; Giuseppe Cinelli; Francesco Lopez; Tiziana Di Renzo; Raffaele Coppola; Anna Reale. Alginate-Assisted Lemongrass (Cymbopogon nardus) Essential Oil Dispersions for Antifungal Activity. Foods 2021, 10, 1528 .

AMA Style

Martina Cofelice, Giuseppe Cinelli, Francesco Lopez, Tiziana Di Renzo, Raffaele Coppola, Anna Reale. Alginate-Assisted Lemongrass (Cymbopogon nardus) Essential Oil Dispersions for Antifungal Activity. Foods. 2021; 10 (7):1528.

Chicago/Turabian Style

Martina Cofelice; Giuseppe Cinelli; Francesco Lopez; Tiziana Di Renzo; Raffaele Coppola; Anna Reale. 2021. "Alginate-Assisted Lemongrass (Cymbopogon nardus) Essential Oil Dispersions for Antifungal Activity." Foods 10, no. 7: 1528.

Journal article
Published: 22 June 2021 in eLife
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Volatile organic compounds (VOCs) from ‘emitting’ plants inform the ‘receiving’ (listening) plants of impending stresses or simply of their presence. However, the receptors that allow receivers to detect the volatile cue are elusive. Most likely, plants (as animals) have odorant-binding proteins (OBPs), and in fact, a few OBPs are known to bind ‘stress-induced’ plant VOCs. We investigated whether these and other putative OBPs may bind volatile constitutive and stress-induced isoprenoids, the most emitted plant VOCs, with well-established roles in plant communication and defense. Molecular docking simulation experiments suggest that structural features of a few plant proteins screened in databases could allow VOC binding. In particular, our results show that monoterpenes may bind the same plant proteins that were described to bind other stress-induced VOCs, while the constitutive hemiterpene isoprene is unlikely to bind any investigated putative OBP and may not have an info-chemical role. We conclude that, as for animal, there may be plant OBPs that bind multiple VOCs. Plant OBPs may play an important role in allowing plants to eavesdrop messages by neighboring plants, triggering defensive responses and communication with other organisms.

ACS Style

Deborah Giordano; Angelo Facchiano; Sabato D'Auria; Francesco Loreto. A hypothesis on the capacity of plant odorant-binding proteins to bind volatile isoprenoids based on in silico evidences. eLife 2021, 10, 1 .

AMA Style

Deborah Giordano, Angelo Facchiano, Sabato D'Auria, Francesco Loreto. A hypothesis on the capacity of plant odorant-binding proteins to bind volatile isoprenoids based on in silico evidences. eLife. 2021; 10 ():1.

Chicago/Turabian Style

Deborah Giordano; Angelo Facchiano; Sabato D'Auria; Francesco Loreto. 2021. "A hypothesis on the capacity of plant odorant-binding proteins to bind volatile isoprenoids based on in silico evidences." eLife 10, no. : 1.

Journal article
Published: 13 June 2021 in International Journal of Molecular Sciences
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Nicotinamide mononucleotide (NMN) is a key intermediate in the nicotinamide adenine dinucleotide (NAD+) biosynthesis. Its supplementation has demonstrated beneficial effects on several diseases. The aim of this study was to characterize NMN deamidase (PncC) inactive mutants to use as possible molecular recognition elements (MREs) for an NMN-specific biosensor. Thermal stability assays and steady-state fluorescence spectroscopy measurements were used to study the binding of NMN and related metabolites (NaMN, Na, Nam, NR, NAD, NADP, and NaAD) to the PncC mutated variants. In particular, the S29A PncC and K61Q PncC variant forms were selected since they still preserve the ability to bind NMN in the micromolar range, but they are not able to catalyze the enzymatic reaction. While S29A PncC shows a similar affinity also for NaMN (the product of the PncC catalyzed reaction), K61Q PncC does not interact significantly with it. Thus, PncC K61Q mutant seems to be a promising candidate to use as specific probe for an NMN biosensor.

ACS Style

Alessandra Camarca; Gabriele Minazzato; Angela Pennacchio; Alessandro Capo; Adolfo Amici; Sabato D’Auria; Nadia Raffaelli. Characterization of Two NMN Deamidase Mutants as Possible Probes for an NMN Biosensor. International Journal of Molecular Sciences 2021, 22, 6334 .

AMA Style

Alessandra Camarca, Gabriele Minazzato, Angela Pennacchio, Alessandro Capo, Adolfo Amici, Sabato D’Auria, Nadia Raffaelli. Characterization of Two NMN Deamidase Mutants as Possible Probes for an NMN Biosensor. International Journal of Molecular Sciences. 2021; 22 (12):6334.

Chicago/Turabian Style

Alessandra Camarca; Gabriele Minazzato; Angela Pennacchio; Alessandro Capo; Adolfo Amici; Sabato D’Auria; Nadia Raffaelli. 2021. "Characterization of Two NMN Deamidase Mutants as Possible Probes for an NMN Biosensor." International Journal of Molecular Sciences 22, no. 12: 6334.

Journal article
Published: 12 May 2021 in Journal of Fungi
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Ascosphaera apis is an entomopathogenic fungus that affects honeybees. In stressful conditions, this fungus (due not only to its presence, but also to the combination of other biotic and abiotic stressors) can cause chalkbrood disease. In recent years, there has been increasing attention paid towards the use of lactic acid bacteria (LAB) in the honeybees’ diets to improve their health, productivity and ability to resist infections by pathogenic microorganisms. The screening of 22 strains of Lactiplantibacillus plantarum, isolated from the gastrointestinal tracts of honeybees and beebread, led to the selection of five strains possessing high antagonistic activity against A. apis. This study focused on the antifungal activity of these five strains against A. apis DSM 3116 and DSM 3117 using different matrices: cell lysate, broth culture, cell-free supernatant and cell pellet. In addition, some functional properties and the antioxidant activity of the five L. plantarum strains were evaluated. All five strains exhibited high antagonistic activity against A. apis, good surface cellular properties (extracellular polysaccharide (EPS) production and biofilm formation) and antioxidant activity. Although preliminary, these results are encouraging, and in future investigations, the effectiveness of these bacteria as probiotics in honeybee nutrition will be tested in vivo in the context of an eco-friendly strategy for the biological control of chalkbrood disease.

ACS Style

Massimo Iorizzo; Bruno Testa; Sonia Ganassi; Silvia Lombardi; Mario Ianiro; Francesco Letizia; Mariantonietta Succi; Patrizio Tremonte; Franca Vergalito; Autilia Cozzolino; Elena Sorrentino; Sonia Petrarca; Antonio De Cristofaro; Raffaele Coppola. Probiotic Properties and Potentiality of Lactiplantibacillus plantarum Strains for the Biological Control of Chalkbrood Disease. Journal of Fungi 2021, 7, 379 .

AMA Style

Massimo Iorizzo, Bruno Testa, Sonia Ganassi, Silvia Lombardi, Mario Ianiro, Francesco Letizia, Mariantonietta Succi, Patrizio Tremonte, Franca Vergalito, Autilia Cozzolino, Elena Sorrentino, Sonia Petrarca, Antonio De Cristofaro, Raffaele Coppola. Probiotic Properties and Potentiality of Lactiplantibacillus plantarum Strains for the Biological Control of Chalkbrood Disease. Journal of Fungi. 2021; 7 (5):379.

Chicago/Turabian Style

Massimo Iorizzo; Bruno Testa; Sonia Ganassi; Silvia Lombardi; Mario Ianiro; Francesco Letizia; Mariantonietta Succi; Patrizio Tremonte; Franca Vergalito; Autilia Cozzolino; Elena Sorrentino; Sonia Petrarca; Antonio De Cristofaro; Raffaele Coppola. 2021. "Probiotic Properties and Potentiality of Lactiplantibacillus plantarum Strains for the Biological Control of Chalkbrood Disease." Journal of Fungi 7, no. 5: 379.

Paper
Published: 10 March 2021 in Lab on a Chip
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A thermostabilized aluminium acoustic trap combined with attenuated total reflection infrared spectroscopy used to perform ultrasound-assisted E. coli detection.

ACS Style

Stephan Freitag; Bettina Baumgartner; Stefan Radel; Andreas Schwaighofer; Antonio Varriale; Angela Pennacchio; Sabato D'Auria; Bernhard Lendl. A thermoelectrically stabilized aluminium acoustic trap combined with attenuated total reflection infrared spectroscopy for detection of Escherichia coli in water. Lab on a Chip 2021, 21, 1811 -1819.

AMA Style

Stephan Freitag, Bettina Baumgartner, Stefan Radel, Andreas Schwaighofer, Antonio Varriale, Angela Pennacchio, Sabato D'Auria, Bernhard Lendl. A thermoelectrically stabilized aluminium acoustic trap combined with attenuated total reflection infrared spectroscopy for detection of Escherichia coli in water. Lab on a Chip. 2021; 21 (9):1811-1819.

Chicago/Turabian Style

Stephan Freitag; Bettina Baumgartner; Stefan Radel; Andreas Schwaighofer; Antonio Varriale; Angela Pennacchio; Sabato D'Auria; Bernhard Lendl. 2021. "A thermoelectrically stabilized aluminium acoustic trap combined with attenuated total reflection infrared spectroscopy for detection of Escherichia coli in water." Lab on a Chip 21, no. 9: 1811-1819.

Preprint content
Published: 03 March 2021
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Plants use odors not only to recruit other organisms for symbioses, but to ‘talk’ to each other. Volatile organic compounds (VOCs) from “emitting” plants inform the “receiving” (listening) plants of impending stresses or simply of their presence. However, the receptors that allow receivers to perceive the volatile cue are elusive. Most likely, plants (as animals) have odorant bind proteins (OBPs), and in fact few OBPs are known to bind “stress-induced” plant VOCs. We investigated whether OBPs may bind volatile constitutive and stress-induced isoprenoids, the most emitted plant VOCs, with well-established roles in plant communication. First, we performed a data base search that generated a list of candidate plant OBPs. Second, we investigated in silico the ability of the identified candidate plant OBPs to bind VOCs by molecular simulation experiments. Our results show that monoterpenes can bind the same OBPs that were described to bind other stress-induced VOCs. Whereas, the constitutive hemiterpene isoprene does not bind any investigated OBP and may not have an info-chemical role. We conclude that, as for animal, plant OBPs may bind different VOCs. Despite being generalist and not specialized, plant OBPs may play an important role in allowing plants to eavesdrop messages sent by neighboring plants.

ACS Style

Deborah Giordano; Angelo Facchiano; Sabato D’Auria; Francesco Loreto. On the capacity of putative plant odorant-binding proteins to bind volatile plant isoprenoids. 2021, 1 .

AMA Style

Deborah Giordano, Angelo Facchiano, Sabato D’Auria, Francesco Loreto. On the capacity of putative plant odorant-binding proteins to bind volatile plant isoprenoids. . 2021; ():1.

Chicago/Turabian Style

Deborah Giordano; Angelo Facchiano; Sabato D’Auria; Francesco Loreto. 2021. "On the capacity of putative plant odorant-binding proteins to bind volatile plant isoprenoids." , no. : 1.

Review
Published: 29 January 2021 in Sensors
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The purpose of this work is to provide an exhaustive overview of the emerging biosensor technologies for the detection of analytes of interest for food, environment, security, and health. Over the years, biosensors have acquired increasing importance in a wide range of applications due to synergistic studies of various scientific disciplines, determining their great commercial potential and revealing how nanotechnology and biotechnology can be strictly connected. In the present scenario, biosensors have increased their detection limit and sensitivity unthinkable until a few years ago. The most widely used biosensors are optical-based devices such as surface plasmon resonance (SPR)-based biosensors and fluorescence-based biosensors. Here, we will review them by highlighting how the progress in their design and development could impact our daily life.

ACS Style

Alessandra Camarca; Antonio Varriale; Alessandro Capo; Angela Pennacchio; Alessia Calabrese; Cristina Giannattasio; Carlos Murillo Almuzara; Sabato D’Auria; Maria Staiano. Emergent Biosensing Technologies Based on Fluorescence Spectroscopy and Surface Plasmon Resonance. Sensors 2021, 21, 906 .

AMA Style

Alessandra Camarca, Antonio Varriale, Alessandro Capo, Angela Pennacchio, Alessia Calabrese, Cristina Giannattasio, Carlos Murillo Almuzara, Sabato D’Auria, Maria Staiano. Emergent Biosensing Technologies Based on Fluorescence Spectroscopy and Surface Plasmon Resonance. Sensors. 2021; 21 (3):906.

Chicago/Turabian Style

Alessandra Camarca; Antonio Varriale; Alessandro Capo; Angela Pennacchio; Alessia Calabrese; Cristina Giannattasio; Carlos Murillo Almuzara; Sabato D’Auria; Maria Staiano. 2021. "Emergent Biosensing Technologies Based on Fluorescence Spectroscopy and Surface Plasmon Resonance." Sensors 21, no. 3: 906.

Proceedings
Published: 04 January 2021 in Proceedings
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Our aim was to evaluate the susceptibility of bacterial biofilm formation and the metabolic changes occurring in the bacterial cells by the action of ivy, strawberry tree, lavender, sulla and tree of heaven monofloral honeys. Listeria monocytogenes was the most sensitive bacteria with percentages of biofilm inhibition up to 72.20%. Pseudomonas aeruginosa was less sensitive, but tree of heaven and sulla honey caused an inhibition of biofilm up to 40.41% and 35.85%, respectively. The tree of heaven honey acted on the P. aeruginosa metabolism (75.24%). Staphylococcus aureus, majorly resistant to the biofilm-inhibitory action of the honey, was more sensitive at the metabolic level (61.63% inhibition in the presence of the tree of heaven honey).

ACS Style

Filomena Nazzaro; Florinda Fratianni; Antonio D’Acierno; Maria Neve Ombra; Lucia Caputo; Raffaele Coppola; Vincenzo De Feo. Anti-Biofilm Properties Exhibited by Different Types of Monofloral Honey. Proceedings 2021, 66, 16 .

AMA Style

Filomena Nazzaro, Florinda Fratianni, Antonio D’Acierno, Maria Neve Ombra, Lucia Caputo, Raffaele Coppola, Vincenzo De Feo. Anti-Biofilm Properties Exhibited by Different Types of Monofloral Honey. Proceedings. 2021; 66 (1):16.

Chicago/Turabian Style

Filomena Nazzaro; Florinda Fratianni; Antonio D’Acierno; Maria Neve Ombra; Lucia Caputo; Raffaele Coppola; Vincenzo De Feo. 2021. "Anti-Biofilm Properties Exhibited by Different Types of Monofloral Honey." Proceedings 66, no. 1: 16.

Journal article
Published: 10 December 2020 in Scientific Reports
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Listeria monocytogenesis a foodborne pathogen responsible for human listeriosis. The increasing incidence of listeriosis induced governments and food manufacturing enterprises to act to diminish the problem. Several methods for the detection ofListeria monocytogenesin food industries were developed. However, they are time-consuming and require the use of specialized equipment. To reduce the detection time ofListeria monocytogenesin food, in this work we developed a fluorescence sandwich immunoassay based on the use of an innovative chitosan-cellulose nanocrystal (CNC) membrane that improves the antigen capture during bacterial growth. The combined use of CNC film for the capture of p60 protein-specific antigen together with the use of fluorescence detection reduced the time of analysis from 24 to 12 h with a limit of detection (LOD) of the assay of 102 CFU/mL (2 Log). In addition, the use of monoclonal anti-PepD covalently immobilized to a CNC membrane assured a high specificity of the assay. Interestingly, the obtained results show no cross-reactivity with the five most diffused pathogen bacteria strains tested.

ACS Style

Alessandro Capo; Sabato D’Auria; Monique Lacroix. A fluorescence immunoassay for a rapid detection of Listeria monocytogenes on working surfaces. Scientific Reports 2020, 10, 1 -12.

AMA Style

Alessandro Capo, Sabato D’Auria, Monique Lacroix. A fluorescence immunoassay for a rapid detection of Listeria monocytogenes on working surfaces. Scientific Reports. 2020; 10 (1):1-12.

Chicago/Turabian Style

Alessandro Capo; Sabato D’Auria; Monique Lacroix. 2020. "A fluorescence immunoassay for a rapid detection of Listeria monocytogenes on working surfaces." Scientific Reports 10, no. 1: 1-12.

Journal article
Published: 26 September 2020 in International Journal of Molecular Sciences
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The synthesis of two 5′-end (4-dimethylamino)azobenzene conjugated G-quadruplex forming aptamers, the thrombin binding aptamer (TBA) and the HIV-1 integrase aptamer (T30695), was performed. Their structural behavior was investigated by means of UV, CD, fluorescence spectroscopy, and gel electrophoresis techniques in K+-containing buffers and water-ethanol blends. Particularly, we observed that the presence of the 5′-(4-dimethylamino)azobenzene moiety leads TBA to form multimers instead of the typical monomolecular chair-like G-quadruplex and almost hampers T30695 G-quadruplex monomers to dimerize. Fluorescence studies evidenced that both the conjugated G-quadruplexes possess unique fluorescence features when excited at wavelengths corresponding to the UV absorption of the conjugated moiety. Furthermore, a preliminary investigation of the trans-cis conversion of the dye incorporated at the 5′-end of TBA and T30695 showed that, unlike the free dye, in K+-containing water-ethanol-triethylamine blend the trans-to-cis conversion was almost undetectable by means of a standard UV spectrophotometer.

ACS Style

Concetta Imperatore; Antonio Varriale; Elisa Rivieccio; Angela Pennacchio; Maria Staiano; Sabato D’Auria; Marcello Casertano; Carlo Altucci; MohammadHassan Valadan; Manjot Singh; Marialuisa Menna; Michela Varra. Spectroscopic Properties of Two 5′-(4-Dimethylamino)Azobenzene Conjugated G-Quadruplex Forming Oligonucleotides. International Journal of Molecular Sciences 2020, 21, 7103 .

AMA Style

Concetta Imperatore, Antonio Varriale, Elisa Rivieccio, Angela Pennacchio, Maria Staiano, Sabato D’Auria, Marcello Casertano, Carlo Altucci, MohammadHassan Valadan, Manjot Singh, Marialuisa Menna, Michela Varra. Spectroscopic Properties of Two 5′-(4-Dimethylamino)Azobenzene Conjugated G-Quadruplex Forming Oligonucleotides. International Journal of Molecular Sciences. 2020; 21 (19):7103.

Chicago/Turabian Style

Concetta Imperatore; Antonio Varriale; Elisa Rivieccio; Angela Pennacchio; Maria Staiano; Sabato D’Auria; Marcello Casertano; Carlo Altucci; MohammadHassan Valadan; Manjot Singh; Marialuisa Menna; Michela Varra. 2020. "Spectroscopic Properties of Two 5′-(4-Dimethylamino)Azobenzene Conjugated G-Quadruplex Forming Oligonucleotides." International Journal of Molecular Sciences 21, no. 19: 7103.

Journal article
Published: 24 July 2020 in Antibiotics
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Paenibacillus larvae is the causative agent of American foulbrood (AFB), a severe bacterial disease that affects larvae of honeybees. The present study evaluated, in vitro, antimicrobial activity of sixty‐one Lactiplantibacillus plantarum strains, against P. larvae ATCC 9545. Five strains (P8, P25, P86, P95 and P100) that showed the greatest antagonism against P. larvae ATCC 9545 were selected for further physiological and biochemical characterizations. In particular, the hydrophobicity, auto‐aggregation, exopolysaccharides production, osmotic tolerance, enzymatic activity and carbohydrate assimilation patterns were evaluated. The five L. plantarum selected strains showed suitable physical and biochemical properties for their use as probiotics in the honeybee diet. The selection and availability of new selected bacteria with good functional characteristics and with antagonistic activity against P. larvae opens up interesting perspectives for new biocontrol strategies of diseases such as AFB.

ACS Style

Massimo Iorizzo; Bruno Testa; Silvia Jane Lombardi; Sonia Ganassi; Mario Ianiro; Francesco Letizia; Mariantonietta Succi; Patrizio Tremonte; Franca Vergalito; Autilia Cozzolino; Elena Sorrentino; Raffaele Coppola; Sonia Petrarca; Massimo Mancini; Antonio De Cristofaro. Antimicrobial Activity against Paenibacillus larvae and Functional Properties of Lactiplantibacillus plantarum Strains: Potential Benefits for Honeybee Health. Antibiotics 2020, 9, 442 .

AMA Style

Massimo Iorizzo, Bruno Testa, Silvia Jane Lombardi, Sonia Ganassi, Mario Ianiro, Francesco Letizia, Mariantonietta Succi, Patrizio Tremonte, Franca Vergalito, Autilia Cozzolino, Elena Sorrentino, Raffaele Coppola, Sonia Petrarca, Massimo Mancini, Antonio De Cristofaro. Antimicrobial Activity against Paenibacillus larvae and Functional Properties of Lactiplantibacillus plantarum Strains: Potential Benefits for Honeybee Health. Antibiotics. 2020; 9 (8):442.

Chicago/Turabian Style

Massimo Iorizzo; Bruno Testa; Silvia Jane Lombardi; Sonia Ganassi; Mario Ianiro; Francesco Letizia; Mariantonietta Succi; Patrizio Tremonte; Franca Vergalito; Autilia Cozzolino; Elena Sorrentino; Raffaele Coppola; Sonia Petrarca; Massimo Mancini; Antonio De Cristofaro. 2020. "Antimicrobial Activity against Paenibacillus larvae and Functional Properties of Lactiplantibacillus plantarum Strains: Potential Benefits for Honeybee Health." Antibiotics 9, no. 8: 442.

Journal article
Published: 10 July 2020 in Microorganisms
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The present study, considering for the first time microbiological concerns due to the use of lemon albedo as a fat replacer, aimed at the selection of an anti-Listeria strain to be used as protective culture in low-fat southern Italian fermented sausages. In fact, these kinds of products require appropriate bio-protective strategies to avoid risks due to Listeria monocytogenes. Sixty-seven Lactiplantibacillus plantarum strains isolated from diverse sources were screened for their antimicrobial activity and their interaction with starter strains (Latilactobacillus sakei 152 and Staphylococcus xylosus MVS9). Lactiplantibacillus plantarum Lpls100, highlighting both listericidal activity and the ability to promote Staphylococcus xylosus MVS9 growth, was used as a protective strain in low-fat fermented sausages prepared with lemon albedo as a fat replacer. The effect of the albedo and the protective strain on the fermentation process and the final quality was ascertained. Results highlighted that the use of the albedo did not affect the growth of starter strains and enhanced some quality features, such as fatty acid profiles and certain sensory attributes. However, the albedo also produced a slow decrease in water activity, compromising the microbial quality. The anti-Listeria strain, enhancing coagulase negative cocci growth and exerting antimicrobial activity, avoided the inconveniences caused by the use of the albedo. Moreover, the anti-Listeria effectiveness was assessed through a challenge test using a Listeria cocktail. The study revealed that Lactiplantibacillus plantarum Lpls100, regardless of the presence of the albedo, assures a prompt inhibition of Listeria spp. Therefore, its use could be an important contribution to the quality of low-fat fermented sausages.

ACS Style

Patrizio Tremonte; Gianfranco Pannella; Silvia Lombardi; Massimo Iorizzo; Franca Vergalito; Autilia Cozzolino; Lucia Maiuro; Mariantonietta Succi; Elena Sorrentino; Raffaele Coppola. Low-Fat and High-Quality Fermented Sausages. Microorganisms 2020, 8, 1025 .

AMA Style

Patrizio Tremonte, Gianfranco Pannella, Silvia Lombardi, Massimo Iorizzo, Franca Vergalito, Autilia Cozzolino, Lucia Maiuro, Mariantonietta Succi, Elena Sorrentino, Raffaele Coppola. Low-Fat and High-Quality Fermented Sausages. Microorganisms. 2020; 8 (7):1025.

Chicago/Turabian Style

Patrizio Tremonte; Gianfranco Pannella; Silvia Lombardi; Massimo Iorizzo; Franca Vergalito; Autilia Cozzolino; Lucia Maiuro; Mariantonietta Succi; Elena Sorrentino; Raffaele Coppola. 2020. "Low-Fat and High-Quality Fermented Sausages." Microorganisms 8, no. 7: 1025.

Journal article
Published: 26 June 2020 in Antioxidants
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Bioactive compounds of different Campania native sweet pepper varieties were evaluated. Polyphenols ranged between 1.37 mmol g–1 and 3.42 mmol g–1, β-carotene was abundant in the red variety “Cazzone” (7.05 μg g–1). Yellow and red varieties showed a content of ascorbic acid not inferior to 0.82 mg g–1, while in some green varieties the presence of ascorbic acid was almost inconsistent. Interrelationships between the parameters analyzed and the varieties showed that ascorbic acid could represent the factor mostly influencing the antioxidant activity. Polyphenol profile was different among the varieties, with a general prevalence of acidic phenols in yellow varieties and of flavonoids in red varieties. Principal Component Analysis, applied to ascorbic acid, total polyphenols and β-carotene, revealed that two of the green varieties (“Friariello napoletano” and “Friariello Sigaretta”) were well clustered and that the yellow variety “Corno di capra” showed similarity with the green varieties, in particular with “Friariello Nocerese”. This was confirmed by the interrelationships applied to polyphenol composition, which let us to light on a clustering of several red and yellow varieties, and that mainly the yellow ”Corno di capra” was closer to the green varieties of “Friariello”.

ACS Style

Florinda Fratianni; Antonio D’Acierno; Autilia Cozzolino; Patrizia Spigno; Riccardo Riccardi; Francesco Raimo; Catello Pane; Massimo Zaccardelli; Valentina Tranchida Lombardo; Marina Tucci; Stefania Grillo; Raffaele Coppola; Filomena Nazzaro. Biochemical Characterization of Traditional Varieties of Sweet Pepper (Capsicum annuum L.) of the Campania Region, Southern Italy. Antioxidants 2020, 9, 556 .

AMA Style

Florinda Fratianni, Antonio D’Acierno, Autilia Cozzolino, Patrizia Spigno, Riccardo Riccardi, Francesco Raimo, Catello Pane, Massimo Zaccardelli, Valentina Tranchida Lombardo, Marina Tucci, Stefania Grillo, Raffaele Coppola, Filomena Nazzaro. Biochemical Characterization of Traditional Varieties of Sweet Pepper (Capsicum annuum L.) of the Campania Region, Southern Italy. Antioxidants. 2020; 9 (6):556.

Chicago/Turabian Style

Florinda Fratianni; Antonio D’Acierno; Autilia Cozzolino; Patrizia Spigno; Riccardo Riccardi; Francesco Raimo; Catello Pane; Massimo Zaccardelli; Valentina Tranchida Lombardo; Marina Tucci; Stefania Grillo; Raffaele Coppola; Filomena Nazzaro. 2020. "Biochemical Characterization of Traditional Varieties of Sweet Pepper (Capsicum annuum L.) of the Campania Region, Southern Italy." Antioxidants 9, no. 6: 556.

Journal article
Published: 25 June 2020 in International Journal of Biological Macromolecules
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L-glutamate (Glu) is the major excitatory transmitter in mammalian brain. Inadequate concentration of Glu in the brain correlates to mood disorder. In industry, Glu is used as a flavour enhancer in food and in foodstuff processing. A high concentration of Glu has several effects on human health such as hypersensitive effects, headache and stomach pain. The presence of Glu in food can be detected by different analytical methods based on chromatography, or capillary electrophoresis or amperometric techniques. We have isolated and characterized a glutamate-binding protein (GluB) from the Gram-positive bacteria Corynebacterium glutamicum. Together with GluC protein, GluD protein and the cytoplasmic protein GluA, GluB permits the transport of Glu in/out of cell. In this study, we have investigated the binding features of GluB as well as the effect of temperature on its structure both in the absence and in the presence of Glu. The results have showed that GluB has a high affinity and selectivity versus Glu (nanomolar range) and the presence of the ligand induces a higher thermal stability of the protein structure.

ACS Style

Alessandro Capo; Antonino Natalello; Jan Marienhagen; Angela Pennacchio; Alessandra Camarca; Stefano Di Giovanni; Maria Staiano; Sabato D'auria; Antonio Varriale. Structural features of the glutamate-binding protein from Corynebacterium glutamicum. International Journal of Biological Macromolecules 2020, 162, 903 -912.

AMA Style

Alessandro Capo, Antonino Natalello, Jan Marienhagen, Angela Pennacchio, Alessandra Camarca, Stefano Di Giovanni, Maria Staiano, Sabato D'auria, Antonio Varriale. Structural features of the glutamate-binding protein from Corynebacterium glutamicum. International Journal of Biological Macromolecules. 2020; 162 ():903-912.

Chicago/Turabian Style

Alessandro Capo; Antonino Natalello; Jan Marienhagen; Angela Pennacchio; Alessandra Camarca; Stefano Di Giovanni; Maria Staiano; Sabato D'auria; Antonio Varriale. 2020. "Structural features of the glutamate-binding protein from Corynebacterium glutamicum." International Journal of Biological Macromolecules 162, no. : 903-912.

Journal article
Published: 17 June 2020 in Foods
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Biofilm life-style of Lactobacillus plantarum (L. plantarum) strains was evaluated in vitro as a new and suitable biotechnological strategy to assure L-malic acid conversion in wine stress conditions. Sixty-eight L. plantarum strains isolated from diverse sources were assessed for their ability to form biofilm in acid (pH 3.5 or 3.2) or in ethanol (12% or 14%) stress conditions. The effect of incubation times (24 and 72 h) on the biofilm formation was evaluated. The study highlighted that, regardless of isolation source and stress conditions, the ability to form biofilm was strain-dependent. Specifically, two clusters, formed by high and low biofilm producer strains, were identified. Among high producer strains, L. plantarum Lpls22 was chosen as the highest producer strain and cultivated in planktonic form or in biofilm using oak supports. Model wines at 12% of ethanol and pH 3.5 or 3.2 were used to assess planktonic and biofilm cells survival and to evaluate the effect of biofilm on L-malic acid conversion. For cells in planktonic form, a strong survival decay was detected. In contrast, cells in biofilm life-style showed high resistance, assuring a prompt and complete L-malic acid conversion.

ACS Style

Gianfranco Pannella; Silvia Jane Lombardi; Francesca Coppola; Franca Vergalito; Massimo Iorizzo; Mariantonietta Succi; Patrizio Tremonte; Caterina Iannini; Elena Sorrentino; Raffaele Coppola. Effect of Biofilm Formation by Lactobacillus plantarum on the Malolactic Fermentation in Model Wine. Foods 2020, 9, 797 .

AMA Style

Gianfranco Pannella, Silvia Jane Lombardi, Francesca Coppola, Franca Vergalito, Massimo Iorizzo, Mariantonietta Succi, Patrizio Tremonte, Caterina Iannini, Elena Sorrentino, Raffaele Coppola. Effect of Biofilm Formation by Lactobacillus plantarum on the Malolactic Fermentation in Model Wine. Foods. 2020; 9 (6):797.

Chicago/Turabian Style

Gianfranco Pannella; Silvia Jane Lombardi; Francesca Coppola; Franca Vergalito; Massimo Iorizzo; Mariantonietta Succi; Patrizio Tremonte; Caterina Iannini; Elena Sorrentino; Raffaele Coppola. 2020. "Effect of Biofilm Formation by Lactobacillus plantarum on the Malolactic Fermentation in Model Wine." Foods 9, no. 6: 797.

Journal article
Published: 18 May 2020 in Antibiotics
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Lactic acid bacteria (LAB) are an important group of honeybee gut microbiota. These bacteria are involved in food digestion, stimulate the immune system, and may antagonize undesirable microorganisms in the gastrointestinal tract. Lactobacillus kunkeei is a fructophilic lactic acid bacterium (FLAB) most frequently found in the gastrointestinal tracts of honeybees. Ascosphaera apis is an important pathogenic fungus of honeybee larvae; it can colonize the intestine, especially in conditions of nutritional or environmental stress that cause microbial dysbiosis. In this work, some functional properties of nine selected L. kunkeei strains were evaluated. The study focused on the antifungal activity of these strains against A. apis DSM 3116, using different matrices: cell lysate, broth culture, cell-free supernatant, and cell pellet. The cell lysate showed the highest antifungal activity. Moreover, the strains were shown to possess good cell-surface properties (hydrophobicity, auto-aggregation, and biofilm production) and a good resistance to high sugar concentrations. These L. kunkeei strains were demonstrated to be functional for use in “probiotic syrup”, useful to restore the symbiotic communities of the intestine in case of dysbiosis and to exert a prophylactic action against A. apis.

ACS Style

Massimo Iorizzo; Silvia Jane Lombardi; Sonia Ganassi; Bruno Testa; Mario Ianiro; Francesco Letizia; Mariantonietta Succi; Patrizio Tremonte; Franca Vergalito; Autilia Cozzolino; Elena Sorrentino; Raffaele Coppola; Sonia Petrarca; Massimo Mancini; Antonio De Cristofaro. Antagonistic Activity against Ascosphaera apis and Functional Properties of Lactobacillus kunkeei Strains. Antibiotics 2020, 9, 262 .

AMA Style

Massimo Iorizzo, Silvia Jane Lombardi, Sonia Ganassi, Bruno Testa, Mario Ianiro, Francesco Letizia, Mariantonietta Succi, Patrizio Tremonte, Franca Vergalito, Autilia Cozzolino, Elena Sorrentino, Raffaele Coppola, Sonia Petrarca, Massimo Mancini, Antonio De Cristofaro. Antagonistic Activity against Ascosphaera apis and Functional Properties of Lactobacillus kunkeei Strains. Antibiotics. 2020; 9 (5):262.

Chicago/Turabian Style

Massimo Iorizzo; Silvia Jane Lombardi; Sonia Ganassi; Bruno Testa; Mario Ianiro; Francesco Letizia; Mariantonietta Succi; Patrizio Tremonte; Franca Vergalito; Autilia Cozzolino; Elena Sorrentino; Raffaele Coppola; Sonia Petrarca; Massimo Mancini; Antonio De Cristofaro. 2020. "Antagonistic Activity against Ascosphaera apis and Functional Properties of Lactobacillus kunkeei Strains." Antibiotics 9, no. 5: 262.

Journal article
Published: 04 April 2020 in Microorganisms
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Malolactic fermentation (MLF) is a biological process that, in addition to deacidifying, also improves biological stability and changes the chemical and sensorial characteristics of wines. However, multiple biotic and abiotic factors, present in must and wine, make the onset and completion of MLF by indigenous malolactic bacteria or added commercial starters difficult. This work illustrates the metabolic and fermentative dynamics in winemaking Fiano wine, using a commercial starter of Saccharomyces cerevisiae and the selected strain Lactobacillus plantarum M10. In particular, an inoculum of malolactic starter was assessed at the beginning of alcoholic fermentation (early co-inoculum), at half alcoholic fermentation (late co-inoculum), and post alcoholic fermentation (sequential inoculum). The malolactic starter, before its use, was pre-adapted in sub-optimal growth conditions (pH 5.0). In sequential inoculum of the Lb. plantarum M10, even in a wine with high acidity, has confirmed its good technological and enzymatic characteristics, completing the MLF and enriching the wine with desirable volatile compounds.

ACS Style

Silvia Jane Lombardi; Gianfranco Pannella; Massimo Iorizzo; Bruno Testa; Mariantonietta Succi; Patrizio Tremonte; Elena Sorrentino; Massimo Di Renzo; Daniela Strollo; Raffaele Coppola. Inoculum Strategies and Performances of Malolactic Starter Lactobacillus plantarum M10: Impact on Chemical and Sensorial Characteristics of Fiano Wine. Microorganisms 2020, 8, 516 .

AMA Style

Silvia Jane Lombardi, Gianfranco Pannella, Massimo Iorizzo, Bruno Testa, Mariantonietta Succi, Patrizio Tremonte, Elena Sorrentino, Massimo Di Renzo, Daniela Strollo, Raffaele Coppola. Inoculum Strategies and Performances of Malolactic Starter Lactobacillus plantarum M10: Impact on Chemical and Sensorial Characteristics of Fiano Wine. Microorganisms. 2020; 8 (4):516.

Chicago/Turabian Style

Silvia Jane Lombardi; Gianfranco Pannella; Massimo Iorizzo; Bruno Testa; Mariantonietta Succi; Patrizio Tremonte; Elena Sorrentino; Massimo Di Renzo; Daniela Strollo; Raffaele Coppola. 2020. "Inoculum Strategies and Performances of Malolactic Starter Lactobacillus plantarum M10: Impact on Chemical and Sensorial Characteristics of Fiano Wine." Microorganisms 8, no. 4: 516.

Journal article
Published: 12 March 2020 in Scientific Reports
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Detection of ciprofloxacin residues in milk by sensitive and rapid methods is of great interest due to its use in the treatment of dairy livestock health. Current analytical approaches to antibiotics detection, are laboratory-based methods and they are time-consuming and require trained personnel. To cope this problem, we propose an assay, based on fluorescence polarization principle, able to detect the presence of ciprofloxacin in diluted milk sample without any pre-treatment. The proposed method is based on the use of ciprofloxacin-protein conjugate labeled with near infrared fluorescence dye, which upon binding to specific antibody causes an increase of the fluorescence polarization emission signal. The developed assay allows for the detection of ciprofloxacin at a concentration of 1ppb, which represents an amount lower than the maximum residual limit (MRL) of ciprofloxacin in milk, as set by the European Union regulation (100 ppb).

ACS Style

Hiyam El Kojok; Nada El Darra; Mahmoud Khalil; Alessandro Capo; Angela Pennacchio; Maria Staiano; Alessandra Camarca; Sabato D’Auria; Antonio Varriale. Fluorescence polarization assay to detect the presence of traces of ciprofloxacin. Scientific Reports 2020, 10, 1 -6.

AMA Style

Hiyam El Kojok, Nada El Darra, Mahmoud Khalil, Alessandro Capo, Angela Pennacchio, Maria Staiano, Alessandra Camarca, Sabato D’Auria, Antonio Varriale. Fluorescence polarization assay to detect the presence of traces of ciprofloxacin. Scientific Reports. 2020; 10 (1):1-6.

Chicago/Turabian Style

Hiyam El Kojok; Nada El Darra; Mahmoud Khalil; Alessandro Capo; Angela Pennacchio; Maria Staiano; Alessandra Camarca; Sabato D’Auria; Antonio Varriale. 2020. "Fluorescence polarization assay to detect the presence of traces of ciprofloxacin." Scientific Reports 10, no. 1: 1-6.