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A debate is ongoing on the significance and appropriateness of the NOVA classification as a tool for categorizing foods based on their degree of processing. As such, the role of ultra-processed food (UPF) on human health is still not completely understood. With this review, we aimed to investigate the actual level of consumption of UPF across countries and target populations to determine the impact in real contexts. Suitable articles published up to March 2021 were sourced through the PubMed and SCOPUS databases. Overall, 99 studies providing data on the level of UPF consumption expressed as the percentage of total energy intake were identified, for a total of 1,378,454 participants. Most of them were published in Brazil (n = 38) and the United States (n = 15), and the 24 h recall was the most-used tool (n = 63). Analysis of the results revealed that the United States and the United Kingdom were the countries with the highest percent energy intake from UPF (generally >50%), whereas Italy had the lowest levels (about 10%); the latter was inversely associated with adherence to the Mediterranean diet. High variability was also observed based on sex, age, and body mass index, with men, young people, and overweight/obese subjects generally having higher levels of consumption compared to older subjects. Overall, our findings underline the large differences in UPF intake. Since most of the observations derived from studies conducted with food questionnaires are not specifically validated for UPF, further efforts are essential to confirm the results previously obtained and to investigate further the association between UPF consumption and health status, also considering the actual contribution within different dietary patterns, which has been less investigated to date.
Mirko Marino; Federica Puppo; Cristian Del Bo’; Valentina Vinelli; Patrizia Riso; Marisa Porrini; Daniela Martini. A Systematic Review of Worldwide Consumption of Ultra-Processed Foods: Findings and Criticisms. Nutrients 2021, 13, 2778 .
AMA StyleMirko Marino, Federica Puppo, Cristian Del Bo’, Valentina Vinelli, Patrizia Riso, Marisa Porrini, Daniela Martini. A Systematic Review of Worldwide Consumption of Ultra-Processed Foods: Findings and Criticisms. Nutrients. 2021; 13 (8):2778.
Chicago/Turabian StyleMirko Marino; Federica Puppo; Cristian Del Bo’; Valentina Vinelli; Patrizia Riso; Marisa Porrini; Daniela Martini. 2021. "A Systematic Review of Worldwide Consumption of Ultra-Processed Foods: Findings and Criticisms." Nutrients 13, no. 8: 2778.
Epidemiological evidence has confirmed the potential causal relationship between specific dietary factors and non-communicable diseases. However, currently nutrition was shown to be insufficiently integrated into medical education, regardless of the country. Without an adequate nutrition education, it is reasonable to assume that future physicians, as well as other health care professionals, will be not able to provide the highest quality care to patients in preventing and treating non-communicable diseases. Furthermore, the insufficient availability of physicians with specializations in nutrition has posed the basis for the development of non-medical careers in the field of nutrition. The present document was drafting by the Italian College of Academic Nutritionists, MED-49 (ICAN-49), with the aim to provide an overview on the nutritional competency standards covered by several health care professionals (Physicians Clinical Nutrition Specialists, Clinical Dietitians, Professional Clinical Nutrition Specialists, etc) for the prevention of diseases and/or support of pharmacological therapies. The aim of the ICAN 49 is to suggest a major shift in practice opportunities and roles for many nutritionists, especially for the management of the metabolic diseases, and promote a paradigm change: a clinical and educational leadership role for Physician Clinical Nutrition Specialists in the hospital setting.
Arturo Pujia; Tiziana Montalcini; Lorenzo M. Donini; Lucia Frittitta; Fabio Galvano; Andrea Natali; Loris Pironi; Marisa Porrini; Patrizia Riso; Angela Albarosa Rivellese; Diego Russo; Giovanni Scapagnini; Mauro Serafini; Anna Tagliabue; Antonino De Lorenzo. Roles and competencies in the nutritional domain for the management of the metabolic diseases and in the hospital setting. Nutrition, Metabolism and Cardiovascular Diseases 2021, 1 .
AMA StyleArturo Pujia, Tiziana Montalcini, Lorenzo M. Donini, Lucia Frittitta, Fabio Galvano, Andrea Natali, Loris Pironi, Marisa Porrini, Patrizia Riso, Angela Albarosa Rivellese, Diego Russo, Giovanni Scapagnini, Mauro Serafini, Anna Tagliabue, Antonino De Lorenzo. Roles and competencies in the nutritional domain for the management of the metabolic diseases and in the hospital setting. Nutrition, Metabolism and Cardiovascular Diseases. 2021; ():1.
Chicago/Turabian StyleArturo Pujia; Tiziana Montalcini; Lorenzo M. Donini; Lucia Frittitta; Fabio Galvano; Andrea Natali; Loris Pironi; Marisa Porrini; Patrizia Riso; Angela Albarosa Rivellese; Diego Russo; Giovanni Scapagnini; Mauro Serafini; Anna Tagliabue; Antonino De Lorenzo. 2021. "Roles and competencies in the nutritional domain for the management of the metabolic diseases and in the hospital setting." Nutrition, Metabolism and Cardiovascular Diseases , no. : 1.
The effect of coffee and cocoa on oxidative damage to macromolecules has been investigated in several studies, often with controversial results. This study aimed to investigate the effect of one-month consumption of different doses of coffee or cocoa-based products containing coffee on markers of DNA damage and lipid peroxidation in young healthy volunteers. Twenty-one volunteers were randomly assigned into a three-arm, crossover, randomized trial. Subjects were assigned to consume one of the three following treatments: one cup of espresso coffee/day (1C), three cups of espresso coffee/day (3C), and one cup of espresso coffee plus two cocoa-based products containing coffee (PC) twice per day for 1 month. At the end of each treatment, blood samples were collected for the analysis of endogenous and H2O2-induced DNA damage and DNA oxidation catabolites, while urines were used for the analysis of oxylipins. On the whole, four DNA catabolites (cyclic guanosine monophosphate (cGMP), 8-OH-2′-deoxy-guanosine, 8-OH-guanine, and 8-NO2-cGMP) were detected in plasma samples following the one-month intervention. No significant modulation of DNA and lipid damage markers was documented among groups, apart from an effect of time for DNA strand breaks and some markers of lipid peroxidation. In conclusion, the consumption of coffee and cocoa-based confectionery containing coffee was apparently not able to affect oxidative stress markers. More studies are encouraged to better explain the findings obtained and to understand the impact of different dosages of these products on specific target groups.
Daniela Martini; Raúl Domínguez-Perles; Alice Rosi; Michele Tassotti; Donato Angelino; Sonia Medina; Cristian Ricci; Alexandre Guy; Camille Oger; Letizia Gigliotti; Thierry Durand; Mirko Marino; Hans Gottfried-Genieser; Marisa Porrini; Monica Antonini; Alessandra Dei Cas; Riccardo Bonadonna; Federico Ferreres; Francesca Scazzina; Furio Brighenti; Patrizia Riso; Cristian Del Bo’; Pedro Mena; Angel Gil-Izquierdo; Daniele Del Rio. Effect of Coffee and Cocoa-Based Confectionery Containing Coffee on Markers of DNA Damage and Lipid Peroxidation Products: Results from a Human Intervention Study. Nutrients 2021, 13, 2399 .
AMA StyleDaniela Martini, Raúl Domínguez-Perles, Alice Rosi, Michele Tassotti, Donato Angelino, Sonia Medina, Cristian Ricci, Alexandre Guy, Camille Oger, Letizia Gigliotti, Thierry Durand, Mirko Marino, Hans Gottfried-Genieser, Marisa Porrini, Monica Antonini, Alessandra Dei Cas, Riccardo Bonadonna, Federico Ferreres, Francesca Scazzina, Furio Brighenti, Patrizia Riso, Cristian Del Bo’, Pedro Mena, Angel Gil-Izquierdo, Daniele Del Rio. Effect of Coffee and Cocoa-Based Confectionery Containing Coffee on Markers of DNA Damage and Lipid Peroxidation Products: Results from a Human Intervention Study. Nutrients. 2021; 13 (7):2399.
Chicago/Turabian StyleDaniela Martini; Raúl Domínguez-Perles; Alice Rosi; Michele Tassotti; Donato Angelino; Sonia Medina; Cristian Ricci; Alexandre Guy; Camille Oger; Letizia Gigliotti; Thierry Durand; Mirko Marino; Hans Gottfried-Genieser; Marisa Porrini; Monica Antonini; Alessandra Dei Cas; Riccardo Bonadonna; Federico Ferreres; Francesca Scazzina; Furio Brighenti; Patrizia Riso; Cristian Del Bo’; Pedro Mena; Angel Gil-Izquierdo; Daniele Del Rio. 2021. "Effect of Coffee and Cocoa-Based Confectionery Containing Coffee on Markers of DNA Damage and Lipid Peroxidation Products: Results from a Human Intervention Study." Nutrients 13, no. 7: 2399.
Summary Background & aims Longevity also carries its dark side of age-related chronic diseases, dementia being one of the worst and the most prevalent. Since dementia lacks effective treatments, preventing or delaying it is highly desirable. Dietary habits and nutrition have been found to be important modifiable risk factors for many chronic diseases, but evidence on the role of diet on the risk of dementia is still limited, particularly among the very old. Aim of the present work is to study the association of the Mediterranean diet and its components with prevalent and incident dementia in the oldest-old. Methods We analyzed data from the Monzino 80-plus study, a population-based study in subjects 80 years or older in the Varese province, Italy. A validated food frequency questionnaire was used to collect information on 23 different foods consumed in the previous year. A Mediterranean diet score was calculated and its components were classified into tertiles. Multivariable models for dementia prevalence and incidence were adjusted for demographic and clinical characteristics. Results Information on nutrition was available for 1390 subjects in the cross-sectional study and 512 subjects in the longitudinal study, mean respective ages 93 and 92. Greater adherence to Mediterranean diet, greater consumption of eggs, fruits and vegetables, carbohydrates, and greater food intake were associated with a lower prevalence of dementia. Increasing number of portions per week and consumption of legumes significantly decreased the incidence of dementia during the 3.6 year mean follow-up: corresponding hazard ratios of highest vs. lowest tertiles (95% confidence intervals) were 0.66 (0.46–0.95) and 0.68 (0.47–0.97), respectively. Conclusion Oldest-old eating less and having diets with less variety and nutrient density were more frequent among subjects with dementia. The longitudinal analysis confirmed oldest-old subjects who eat more portions, as well as those who have a higher intake of legumes, are at decreased risk of developing dementia even though reverse causality cannot be completely ruled out.
Cristina Nicoli; Alessia Antonella Galbussera; Cristina Bosetti; Carlotta Franchi; Silvano Gallus; Sara Mandelli; Gabriella Marcon; Pierluigi Quadri; Patrizia Riso; Emma Riva; Ugo Lucca; Mauro Tettamanti. The role of diet on the risk of dementia in the oldest old: The Monzino 80-plus population-based study. Clinical Nutrition 2021, 40, 4783 -4791.
AMA StyleCristina Nicoli, Alessia Antonella Galbussera, Cristina Bosetti, Carlotta Franchi, Silvano Gallus, Sara Mandelli, Gabriella Marcon, Pierluigi Quadri, Patrizia Riso, Emma Riva, Ugo Lucca, Mauro Tettamanti. The role of diet on the risk of dementia in the oldest old: The Monzino 80-plus population-based study. Clinical Nutrition. 2021; 40 (7):4783-4791.
Chicago/Turabian StyleCristina Nicoli; Alessia Antonella Galbussera; Cristina Bosetti; Carlotta Franchi; Silvano Gallus; Sara Mandelli; Gabriella Marcon; Pierluigi Quadri; Patrizia Riso; Emma Riva; Ugo Lucca; Mauro Tettamanti. 2021. "The role of diet on the risk of dementia in the oldest old: The Monzino 80-plus population-based study." Clinical Nutrition 40, no. 7: 4783-4791.
Food choices and eating behaviours have a large impact on both human and planetary health. Recently, the Food and Agricultural Organisation (FAO) of the United Nations and the World Health Organisation have developed a list of 16 guiding principles to achieve sustainable healthy diets (SHDs). They proposed that development of food-based dietary guidelines (FBDGs) should be a core element in the implementation of these SHDs in each country. The objective of this review is to explore the degree of alignment of current FBDGs to these guiding principles. A total of 43 FBDGs, written or translated into English, were collected from the online repository developed by the FAO and were analysed for their adherence to each of the guiding principles. Results were stratified for period of publication and geographical macro-area. Overall, there were high levels of inclusion of the factors related to health outcomes, especially in the most recent FBDGs. Conversely, environmental impact and socio-cultural aspects of diet were considered less frequently, especially in the older FBDGs. These results highlight the importance of revising FBDGs, especially to include emerging topics which represent the areas with the highest scope for improvement in the future versions of FBDGs. Replication of the present study in the coming years will be worthwhile to monitor improvements in the adherence of global FBDGs to the guiding principles of SHDs. The attainment of such a goal could promote a more rapid transition towards SHDs, as well as highlighting pivotal research trajectories to increase adoption and evaluate the impact on the food system.
Daniela Martini; Massimiliano Tucci; James Bradfield; Antonio Di Giorgio; Mirko Marino; Cristian Del Bo'; Marisa Porrini; Patrizia Riso. Principles of Sustainable Healthy Diets in Worldwide Dietary Guidelines: Efforts So Far and Future Perspectives. Nutrients 2021, 13, 1827 .
AMA StyleDaniela Martini, Massimiliano Tucci, James Bradfield, Antonio Di Giorgio, Mirko Marino, Cristian Del Bo', Marisa Porrini, Patrizia Riso. Principles of Sustainable Healthy Diets in Worldwide Dietary Guidelines: Efforts So Far and Future Perspectives. Nutrients. 2021; 13 (6):1827.
Chicago/Turabian StyleDaniela Martini; Massimiliano Tucci; James Bradfield; Antonio Di Giorgio; Mirko Marino; Cristian Del Bo'; Marisa Porrini; Patrizia Riso. 2021. "Principles of Sustainable Healthy Diets in Worldwide Dietary Guidelines: Efforts So Far and Future Perspectives." Nutrients 13, no. 6: 1827.
Patrizia Riso. The effect of blueberries on blood vessel function, inflammation and oxidative stress in older people. 2021, 1 .
AMA StylePatrizia Riso. The effect of blueberries on blood vessel function, inflammation and oxidative stress in older people. . 2021; ():1.
Chicago/Turabian StylePatrizia Riso. 2021. "The effect of blueberries on blood vessel function, inflammation and oxidative stress in older people." , no. : 1.
The use of DNA damage as marker of oxidative stress, metabolic dysfunction and age-related diseases is debated. The present study aimed at assessing the level of DNA damage (evaluated as DNA strand-breaks, endogenous and oxidatively-induced DNA damage) in a group of older subjects with intestinal permeability enrolled within the MaPLE (Gut and Blood Microbiomics for Studying the Effect of a Polyphenol-Rich Dietary Pattern on Intestinal Permeability in the Elderly) intervention trial, to evaluate its association with clinical, metabolic and dietary markers. DNA damage in peripheral blood mononuclear cells was assessed by the comet assay in 49 older subjects participating in the study. Clinical and metabolic markers, markers of inflammation, vascular function and intestinal permeability were determined in serum. Food intake was estimated by weighted food diaries. On the whole, a trend towards higher levels of DNA damage was observed in men compared to women (p = 0.071). A positive association between DNA damage and clinical/metabolic markers (e.g., uric acid, lipid profile) and an inverse association with dietary markers (e.g., vitamin C, E, B6, folates) were found and differed based on sex. By considering the importance of DNA stability during aging, the results obtained on sex differences and the potential role of dietary and metabolic factors on DNA damage underline the need for further investigations in a larger group of older adults to confirm the associations found and to promote preventive strategies.
Cristian Del Bo’; Daniela Martini; Stefano Bernardi; Letizia Gigliotti; Mirko Marino; Giorgio Gargari; Tomas Meroño; Nicole Hidalgo-Liberona; Cristina Andres-Lacueva; Paul Kroon; Antonio Cherubini; Simone Guglielmetti; Marisa Porrini; Patrizia Riso. Association between Food Intake, Clinical and Metabolic Markers and DNA Damage in Older Subjects. Antioxidants 2021, 10, 730 .
AMA StyleCristian Del Bo’, Daniela Martini, Stefano Bernardi, Letizia Gigliotti, Mirko Marino, Giorgio Gargari, Tomas Meroño, Nicole Hidalgo-Liberona, Cristina Andres-Lacueva, Paul Kroon, Antonio Cherubini, Simone Guglielmetti, Marisa Porrini, Patrizia Riso. Association between Food Intake, Clinical and Metabolic Markers and DNA Damage in Older Subjects. Antioxidants. 2021; 10 (5):730.
Chicago/Turabian StyleCristian Del Bo’; Daniela Martini; Stefano Bernardi; Letizia Gigliotti; Mirko Marino; Giorgio Gargari; Tomas Meroño; Nicole Hidalgo-Liberona; Cristina Andres-Lacueva; Paul Kroon; Antonio Cherubini; Simone Guglielmetti; Marisa Porrini; Patrizia Riso. 2021. "Association between Food Intake, Clinical and Metabolic Markers and DNA Damage in Older Subjects." Antioxidants 10, no. 5: 730.
Nicole Hidalgo-Liberona; Raúl González-Domínguez; Esteban Vegas; Patrizia Riso; Cristian Del Bo’; Stefano Bernardi; Gregorio Peron; Simone Guglielmetti; Giorgio Gargari; Paul Antony Kroon; Antonio Cherubini; Cristina Andrés-Lacueva. Correction to “Increased Intestinal Permeability in Older Subjects Impacts the Beneficial Effects of Dietary Polyphenols by Modulating Their Bioavailability”. Journal of Agricultural and Food Chemistry 2021, 69, 3266 -3266.
AMA StyleNicole Hidalgo-Liberona, Raúl González-Domínguez, Esteban Vegas, Patrizia Riso, Cristian Del Bo’, Stefano Bernardi, Gregorio Peron, Simone Guglielmetti, Giorgio Gargari, Paul Antony Kroon, Antonio Cherubini, Cristina Andrés-Lacueva. Correction to “Increased Intestinal Permeability in Older Subjects Impacts the Beneficial Effects of Dietary Polyphenols by Modulating Their Bioavailability”. Journal of Agricultural and Food Chemistry. 2021; 69 (10):3266-3266.
Chicago/Turabian StyleNicole Hidalgo-Liberona; Raúl González-Domínguez; Esteban Vegas; Patrizia Riso; Cristian Del Bo’; Stefano Bernardi; Gregorio Peron; Simone Guglielmetti; Giorgio Gargari; Paul Antony Kroon; Antonio Cherubini; Cristina Andrés-Lacueva. 2021. "Correction to “Increased Intestinal Permeability in Older Subjects Impacts the Beneficial Effects of Dietary Polyphenols by Modulating Their Bioavailability”." Journal of Agricultural and Food Chemistry 69, no. 10: 3266-3266.
There is an urgent need to promote healthy and sustainable diets that are tailored to the preferences and cultures of different populations. The present study aimed to (i) define a Mediterranean dietary pattern in line with the EAT-Lancet Commission reference diet (ELCRD), based on 2500 kcal/day and adapted to the Italian food habits (EAT-IT); (ii) develop a mid/long-term dietary plan based on EAT-IT and a dietary plan based on the Italian Dietary Guidelines (IDG); (iii) compare the two dietary plans in terms of portions, frequencies of consumption, and nutritional adequacy based on the nutrient and energy recommendations for the Italian adult population. The main differences between the two plans were related to the higher amount of fruit and vegetables in the IDG compared to the EAT-IT, while the EAT-IT plan was higher in nuts and legumes, which represent the main protein sources in the ELCRD. Differences in the protein sources, especially milk and derivatives, and for cereal-based foods, were also found. Dietary plans were comparable for most nutrients, except for higher energy from lipids and vegetal protein, a higher amount of fiber, and lower levels of calcium that were evidenced for the EAT-IT dietary plan compared to the IDG-based one. In conclusion, the analysis of the EAT-IT demonstrated certain nutritional issues. It remains to be determined whether this may represent a health concern in further studies aimed at investigating the feasibility of sustainable dietary patterns.
Massimiliano Tucci; Daniela Martini; Cristian Del Bo’; Mirko Marino; Alberto Battezzati; Simona Bertoli; Marisa Porrini; Patrizia Riso. An Italian-Mediterranean Dietary Pattern Developed Based on the EAT-Lancet Reference Diet (EAT-IT): A Nutritional Evaluation. Foods 2021, 10, 558 .
AMA StyleMassimiliano Tucci, Daniela Martini, Cristian Del Bo’, Mirko Marino, Alberto Battezzati, Simona Bertoli, Marisa Porrini, Patrizia Riso. An Italian-Mediterranean Dietary Pattern Developed Based on the EAT-Lancet Reference Diet (EAT-IT): A Nutritional Evaluation. Foods. 2021; 10 (3):558.
Chicago/Turabian StyleMassimiliano Tucci; Daniela Martini; Cristian Del Bo’; Mirko Marino; Alberto Battezzati; Simona Bertoli; Marisa Porrini; Patrizia Riso. 2021. "An Italian-Mediterranean Dietary Pattern Developed Based on the EAT-Lancet Reference Diet (EAT-IT): A Nutritional Evaluation." Foods 10, no. 3: 558.
Summary Background & aim Increased intestinal permeability (IP) can occur in older people and contribute to the activation of the immune system and inflammation. Dietary interventions may represent a potential strategy to reduce IP. In this regard, specific food bioactives such as polyphenols have been proposed as potential IP modulator due to their ability to affect several critical targets and pathways that control IP. The trial aimed to test the hypothesis that a polyphenol-rich dietary pattern can decrease serum zonulin levels, an IP surrogate marker involved in tight junction modulation, and can beneficially alter the intestinal microbiota, and IP-associated biochemical and clinical markers in older subjects. Methods A randomised, controlled, cross-over intervention trial was performed. Sixty-six subjects (aged ≥ 60 y) with increased IP based on serum zonulin levels, were randomly allocated to one of the two arms of the intervention consisting of a control diet (C-diet) vs. a polyphenol-rich diet (PR-diet). Each intervention was 8-week long and separated by an 8-week wash out period. At the beginning and at the end of each intervention period, serum samples were collected for the quantification of zonulin and other biological markers. Faecal samples were also collected to investigate the intestinal microbial ecosystem. In addition, anthropometrical/physical/biochemical parameters and food intake were evaluated. Results Fifty-one subjects successfully completed the intervention and a high compliance to the dietary protocols was demonstrated. Overall, polyphenol intake significantly increased from a mean of 812 mg/day in the C diet to 1391 mg/day in the PR-diet. Two-way analysis of variance showed a significant effect of treatment (p = 0.008) and treatment × time interaction (p = 0.025) on serum zonulin levels, which decreased after the 8-week PR-diet. In addition, a treatment × time interaction was observed showing a reduction of diastolic blood pressure (p = 0.028) following the PR-diet, which was strongest in those not using antihypertensive drugs. A decrease in both diastolic (p = 0.043) and systolic blood pressure (p = 0.042) was observed in women. Interestingly, a significant increase in fibre-fermenting and butyrate-producing bacteria such as the family Ruminococcaceae and members of the genus Faecalibacterium was observed following the PR intervention. The efficacy of this dietary intervention was greater in subjects with higher serum zonulin at baseline, who showed more pronounced alterations in the markers under study. Furthermore, zonulin reduction was also stronger among subjects with higher body mass index and with insulin resistance at baseline, thus demonstrating the close interplay between IP and metabolic features. Conclusions These data show, for the first time, that a PR-diet can reduce serum zonulin levels, an indirect marker of IP. In addition, PR-diet reduced blood pressure and increased fibre-fermenting and butyrate-producing bacteria. These findings may represent an initial breakthrough for further intervention studies evaluating possible dietary treatments for the management of IP, inflammation and gut function in different target populations. This study was registered at www.isrctn.org as ISRCTN10214981.
Cristian Del Bo'; Stefano Bernardi; Antonio Cherubini; Marisa Porrini; Giorgio Gargari; Nicole Hidalgo-Liberona; Raúl González-Domínguez; Raul Zamora-Ros; Gregorio Peron; Mirko Marino; Letizia Gigliotti; Mark S. Winterbone; Benjamin Kirkup; Paul A. Kroon; Cristina Andres-Lacueva; Simone Guglielmetti; Patrizia Riso. A polyphenol-rich dietary pattern improves intestinal permeability, evaluated as serum zonulin levels, in older subjects: The MaPLE randomised controlled trial. Clinical Nutrition 2020, 40, 3006 -3018.
AMA StyleCristian Del Bo', Stefano Bernardi, Antonio Cherubini, Marisa Porrini, Giorgio Gargari, Nicole Hidalgo-Liberona, Raúl González-Domínguez, Raul Zamora-Ros, Gregorio Peron, Mirko Marino, Letizia Gigliotti, Mark S. Winterbone, Benjamin Kirkup, Paul A. Kroon, Cristina Andres-Lacueva, Simone Guglielmetti, Patrizia Riso. A polyphenol-rich dietary pattern improves intestinal permeability, evaluated as serum zonulin levels, in older subjects: The MaPLE randomised controlled trial. Clinical Nutrition. 2020; 40 (5):3006-3018.
Chicago/Turabian StyleCristian Del Bo'; Stefano Bernardi; Antonio Cherubini; Marisa Porrini; Giorgio Gargari; Nicole Hidalgo-Liberona; Raúl González-Domínguez; Raul Zamora-Ros; Gregorio Peron; Mirko Marino; Letizia Gigliotti; Mark S. Winterbone; Benjamin Kirkup; Paul A. Kroon; Cristina Andres-Lacueva; Simone Guglielmetti; Patrizia Riso. 2020. "A polyphenol-rich dietary pattern improves intestinal permeability, evaluated as serum zonulin levels, in older subjects: The MaPLE randomised controlled trial." Clinical Nutrition 40, no. 5: 3006-3018.
In recent years, the increasing number of studies on polyphenol demonstrates the efforts in elucidating the potential role of these bioactives on human health. This study reviews the main topics and characteristics of clinical trials on polyphenols registered over the last 20 years, in order to track past and current efforts as well as to highlight the main research gaps in this field. The review was conducted by collecting trials registered in ClinicalTrials.gov and International Standard Randomised Controlled Trial Number (ISRCTN) registry. Overall, 750 clinical trials were selected and included in the final evaluation. Most of the trials were performed on extracts or pure compounds followed by studies conducted on polyphenol-rich foods, in particular berries. A total of 520 clinical trials focused on health effects, 55 on bioavailability, and 175 on both. Regarding outcomes, 139 registered intervention studies had the lipid profile and blood pressure as primary outcomes. The overview provided by this analysis also emphasizes the emerging interest in new outcomes related to polyphenols intervention such as microbiota composition and the evaluation of inter-individual variability in response to the intake of polyphenols. Our review underlines the need of further trials covering unexplored or debated research aspects and provides insights for the design and development of future intervention studies and related research areas.
Mirko Marino; Cristian Del Bo’; Daniela Martini; Marisa Porrini; Patrizia Riso. A Review of Registered Clinical Trials on Dietary (Poly)Phenols: Past Efforts and Possible Future Directions. Foods 2020, 9, 1606 .
AMA StyleMirko Marino, Cristian Del Bo’, Daniela Martini, Marisa Porrini, Patrizia Riso. A Review of Registered Clinical Trials on Dietary (Poly)Phenols: Past Efforts and Possible Future Directions. Foods. 2020; 9 (11):1606.
Chicago/Turabian StyleMirko Marino; Cristian Del Bo’; Daniela Martini; Marisa Porrini; Patrizia Riso. 2020. "A Review of Registered Clinical Trials on Dietary (Poly)Phenols: Past Efforts and Possible Future Directions." Foods 9, no. 11: 1606.
Polyphenols have great potential in regulating intestinal health and ameliorating pathological conditions related to increased intestinal permeability (IP). However, the efficacy of dietary interventions with these phytochemicals may significantly be influenced by interindividual variability factors affecting their bioavailability and consequent biological activity. In the present study, urine samples collected from older subjects undergoing a crossover intervention trial with polyphenol-rich foods were subjected to metabolomics analysis for investigating the impact of increased IP on the bioavailability of polyphenols. Interestingly, urinary levels of phase II and microbiota-derived metabolites were significantly different between subjects with healthier intestinal barrier integrity and those with increased IP disruption. Our results support that this IP-dependent impaired bioavailability of polyphenols could be attributed to disturbances in the gut microbial metabolism and phase II methylation processes. Furthermore, we also observed that microbiota-derived metabolites could be largely responsible for the biological activity elicited by dietary polyphenols against age-related disrupted IP.
Nicole Hidalgo-Liberona; Raúl González-Domínguez; Esteban Vegas; Patrizia Riso; Cristian Del Bo’; Stefano Bernardi; Gregorio Peron; Simone Guglielmetti; Giorgio Gargari; Paul Antony Kroon; Antonio Cherubini; Cristina Andrés-Lacueva. Increased Intestinal Permeability in Older Subjects Impacts the Beneficial Effects of Dietary Polyphenols by Modulating Their Bioavailability. Journal of Agricultural and Food Chemistry 2020, 68, 12476 -12484.
AMA StyleNicole Hidalgo-Liberona, Raúl González-Domínguez, Esteban Vegas, Patrizia Riso, Cristian Del Bo’, Stefano Bernardi, Gregorio Peron, Simone Guglielmetti, Giorgio Gargari, Paul Antony Kroon, Antonio Cherubini, Cristina Andrés-Lacueva. Increased Intestinal Permeability in Older Subjects Impacts the Beneficial Effects of Dietary Polyphenols by Modulating Their Bioavailability. Journal of Agricultural and Food Chemistry. 2020; 68 (44):12476-12484.
Chicago/Turabian StyleNicole Hidalgo-Liberona; Raúl González-Domínguez; Esteban Vegas; Patrizia Riso; Cristian Del Bo’; Stefano Bernardi; Gregorio Peron; Simone Guglielmetti; Giorgio Gargari; Paul Antony Kroon; Antonio Cherubini; Cristina Andrés-Lacueva. 2020. "Increased Intestinal Permeability in Older Subjects Impacts the Beneficial Effects of Dietary Polyphenols by Modulating Their Bioavailability." Journal of Agricultural and Food Chemistry 68, no. 44: 12476-12484.
Aging is a risk factor for the development of multiple chronic diseases, including cardiovascular disease, cancer and dementia. Life expectancy has increased in certain countries but this phenomenon is associated with a reduction of years of healthy life. Aging is associated with a number of physical and functional changes, especially sarcopenia. Sarcopenia is a clinical condition associated with a decrease in skeletal muscle and muscle strength, however, sarcopenia is a reversible condition. On the basis of the current scientific literature, sarcopenia could more appropriately capture an individual’s vulnerability to negative health-related outcomes since it represents an early form of the chronic diseases. Recognition of this clinical condition can improve the management of older individuals in many different clinical settings. Despite the limitations of the indirect methods used to study body composition, the Italian College of the Academic Nutritionists ME/49 recommends that health authorities and health professionals around the world should make a greater effort to diagnose sarcopenia earlier and to manage it more effectively. In line with the development of cancer screening, the use of two diagnostic tools for sarcopenia (BIA and DXA) should be implemented.
Tiziana Montalcini; Arturo Pujia; Lorenzo M. Donini; Lucia Frittitta; Fabio Galvano; Andrea Natali; Loris Pironi; Marisa Porrini; Patrizia Riso; Angela Albarosa Rivellese; Diego Russo; Giovanni Scapagnini; Mauro Serafini; Anna Tagliabue; Antonino De Lorenzo. A Call to Action: Now is the Time to Screen Elderly and Treat Osteosarcopenia, a Position Paper of the Italian College of Academic Nutritionists MED/49 (ICAN-49). Nutrients 2020, 12, 2662 .
AMA StyleTiziana Montalcini, Arturo Pujia, Lorenzo M. Donini, Lucia Frittitta, Fabio Galvano, Andrea Natali, Loris Pironi, Marisa Porrini, Patrizia Riso, Angela Albarosa Rivellese, Diego Russo, Giovanni Scapagnini, Mauro Serafini, Anna Tagliabue, Antonino De Lorenzo. A Call to Action: Now is the Time to Screen Elderly and Treat Osteosarcopenia, a Position Paper of the Italian College of Academic Nutritionists MED/49 (ICAN-49). Nutrients. 2020; 12 (9):2662.
Chicago/Turabian StyleTiziana Montalcini; Arturo Pujia; Lorenzo M. Donini; Lucia Frittitta; Fabio Galvano; Andrea Natali; Loris Pironi; Marisa Porrini; Patrizia Riso; Angela Albarosa Rivellese; Diego Russo; Giovanni Scapagnini; Mauro Serafini; Anna Tagliabue; Antonino De Lorenzo. 2020. "A Call to Action: Now is the Time to Screen Elderly and Treat Osteosarcopenia, a Position Paper of the Italian College of Academic Nutritionists MED/49 (ICAN-49)." Nutrients 12, no. 9: 2662.
The evaluation of food intake in older subjects is crucial in order to be able to verify adherence to nutritional recommendations. In this context, estimation of the intake of specific dietary bioactives, such as polyphenols, although particularly challenging, is necessary to plan possible intervention strategies to increase their intake. The aims of the present study were to: (i) evaluate the nutritional composition of dietary menus provided in a residential care setting; (ii) estimate the actual intake of nutrients and polyphenols in a group of older subjects participating in the MaPLE study; and (iii) investigate the impact of an eight-week polyphenol-rich dietary pattern, compared to an eight-week control diet, on overall nutrient and polyphenol intake in older participants. The menus served to the participants provided ~770 mg per day of total polyphenols on average with small variations between seasons. The analysis of real consumption, measured using weighed food diaries, demonstrated a lower nutrient (~20%) and polyphenol intake (~15%) compared to that provided by the menus. The feasibility of dietary patterns that enable an increase in polyphenol intake with putative health benefits for age-related conditions is discussed, with a perspective to developing dietary guidelines for this target population.
Daniela Martini; Stefano Bernardi; Cristian Del Bo’; Nicole Hidalgo Liberona; Raul Zamora-Ros; Massimiliano Tucci; Antonio Cherubini; Marisa Porrini; Giorgio Gargari; Raúl González-Domínguez; Gregorio Peron; Benjamin Kirkup; Paul A. Kroon; Cristina Andres-Lacueva; Simone Guglielmetti; Patrizia Riso. Estimated Intakes of Nutrients and Polyphenols in Participants Completing the MaPLE Randomised Controlled Trial and Its Relevance for the Future Development of Dietary Guidelines for the Older Subjects. Nutrients 2020, 12, 2458 .
AMA StyleDaniela Martini, Stefano Bernardi, Cristian Del Bo’, Nicole Hidalgo Liberona, Raul Zamora-Ros, Massimiliano Tucci, Antonio Cherubini, Marisa Porrini, Giorgio Gargari, Raúl González-Domínguez, Gregorio Peron, Benjamin Kirkup, Paul A. Kroon, Cristina Andres-Lacueva, Simone Guglielmetti, Patrizia Riso. Estimated Intakes of Nutrients and Polyphenols in Participants Completing the MaPLE Randomised Controlled Trial and Its Relevance for the Future Development of Dietary Guidelines for the Older Subjects. Nutrients. 2020; 12 (8):2458.
Chicago/Turabian StyleDaniela Martini; Stefano Bernardi; Cristian Del Bo’; Nicole Hidalgo Liberona; Raul Zamora-Ros; Massimiliano Tucci; Antonio Cherubini; Marisa Porrini; Giorgio Gargari; Raúl González-Domínguez; Gregorio Peron; Benjamin Kirkup; Paul A. Kroon; Cristina Andres-Lacueva; Simone Guglielmetti; Patrizia Riso. 2020. "Estimated Intakes of Nutrients and Polyphenols in Participants Completing the MaPLE Randomised Controlled Trial and Its Relevance for the Future Development of Dietary Guidelines for the Older Subjects." Nutrients 12, no. 8: 2458.
Iron is a fundamental element in human history, from the dawn of civilization to contemporary days. The ancients used the metal to shape tools, to forge weapons, and even as a dietary supplement. This last indication has been handed down until today, when martial therapy is considered fundamental to correct deficiency states of anemia. The improvement of the martial status is mainly targeted with dietary supplements that often couple diverse co-factors, but other methods are available, such as parenteral preparations, dietary interventions, or real-world approaches. The oral absorption of this metal occurs in the duodenum and is highly dependent upon its oxidation state, with many absorption influencers possibly interfering with the intestinal uptake. Bone marrow and spleen represent the initial and ultimate step of iron metabolism, respectively, and the most part of body iron circulates bound to specific proteins and mainly serves to synthesize hemoglobin for new red blood cells. Whatever the martial status is, today’s knowledge about iron biochemistry allows us to embrace exceedingly personalized interventions, which however owe their success to the mythical and historical events that always accompanied this metal.
Matteo Briguglio; Silvana Hrelia; Marco Malaguti; Giovanni Lombardi; Patrizia Riso; Marisa Porrini; Paolo Perazzo; Giuseppe Banfi. The Central Role of Iron in Human Nutrition: From Folk to Contemporary Medicine. Nutrients 2020, 12, 1761 .
AMA StyleMatteo Briguglio, Silvana Hrelia, Marco Malaguti, Giovanni Lombardi, Patrizia Riso, Marisa Porrini, Paolo Perazzo, Giuseppe Banfi. The Central Role of Iron in Human Nutrition: From Folk to Contemporary Medicine. Nutrients. 2020; 12 (6):1761.
Chicago/Turabian StyleMatteo Briguglio; Silvana Hrelia; Marco Malaguti; Giovanni Lombardi; Patrizia Riso; Marisa Porrini; Paolo Perazzo; Giuseppe Banfi. 2020. "The Central Role of Iron in Human Nutrition: From Folk to Contemporary Medicine." Nutrients 12, no. 6: 1761.
The increased presence of bacteria in blood is a plausible contributing factor in the development and progression of aging-associated diseases. In this context, we performed the quantification and the taxonomic profiling of the bacterial DNA in blood samples collected from a group of forty-three older subjects enrolled in a nursing home. Quantitative PCR targeting the 16S rRNA gene revealed that all the older volunteers contained detectable amounts of bacterial DNA in their blood. The total amount of 16S rRNA gene copies varied considerably between subjects. Correlation analyses revealed that the bacterial DNAemia (expressed as concentration of 16S rRNA gene copies in blood) significantly correlated with the serum levels of zonulin, an emerging marker of intestinal permeability. This result was confirmed by the analysis of a second set of blood samples collected after approximately four months from the same subjects. Analyses of 16S rRNA gene profiling revealed that most of the bacterial DNA detected in blood was ascribable to the phylum Proteobacteria with a predominance of Pseudomonadaceae and Enterobacteriaceae. Several control samples were also analyzed to assess the influence exerted by contaminant bacterial DNA potentially originating from reagents and materials. The date reported here suggest that para-cellular permeability of epithelial (and potentially also endothelial) cell layers may play an important role in bacterial migration into the bloodstream. Bacterial DNAemia is likely to impact on several aspects of host physiology and could underpin the development and prognosis of various diseases in older subjects.
Giorgio Gargari; Valentina Taverniti; Cristian Del Bo’; Stefano Bernardi; Cristina Andres-Lacueva; Raul Gonzalez-Dominguez; Paul A. Kroon; Mark S. Winterbone; Antonio Cherubini; Patrizia Riso; Simone Guglielmetti. Bacterial DNAemia is associated with serum zonulin levels in older subjects. 2020, 1 .
AMA StyleGiorgio Gargari, Valentina Taverniti, Cristian Del Bo’, Stefano Bernardi, Cristina Andres-Lacueva, Raul Gonzalez-Dominguez, Paul A. Kroon, Mark S. Winterbone, Antonio Cherubini, Patrizia Riso, Simone Guglielmetti. Bacterial DNAemia is associated with serum zonulin levels in older subjects. . 2020; ():1.
Chicago/Turabian StyleGiorgio Gargari; Valentina Taverniti; Cristian Del Bo’; Stefano Bernardi; Cristina Andres-Lacueva; Raul Gonzalez-Dominguez; Paul A. Kroon; Mark S. Winterbone; Antonio Cherubini; Patrizia Riso; Simone Guglielmetti. 2020. "Bacterial DNAemia is associated with serum zonulin levels in older subjects." , no. : 1.
The present study aims to evaluate the ability of peonidin and petunidin-3-glucoside (Peo-3-glc and Pet-3-glc) and their metabolites (vanillic acid; VA and methyl-gallic acid; MetGA), to prevent monocyte (THP-1) adhesion to endothelial cells (HUVECs), and to reduce the production of vascular cell adhesion molecule (VCAM)-1, E-selectin and vascular endothelial growth factor (VEGF) in a stimulated pro-inflammatory environment, a pivotal step of atherogenesis. Tumor necrosis factor-α (TNF-α; 100 ng mL−1) was used to stimulate the adhesion of labelled monocytes (THP-1) to endothelial cells (HUVECs). Successively, different concentrations of Peo-3-glc and Pet-3-glc (0.02 µM, 0.2 µM, 2 µM and 20 µM), VA and MetGA (0.05 µM, 0.5 µM, 5 µM and 50 µM) were tested. After 24 h, VCAM-1, E-selectin and VEGF were quantified by ELISA, while the adhesion process was measured spectrophotometrically. Peo-3-glc and Pet-3-glc (from 0.02 µM to 20 µM) significantly (p < 0.0001) decreased THP-1 adhesion to HUVECs at all concentrations (−37%, −24%, −30% and −47% for Peo-3-glc; −37%, −33%, −33% and −45% for Pet-3-glc). VA, but not MetGA, reduced the adhesion process at 50 µM (−21%; p < 0.001). At the same concentrations, a significant (p < 0.0001) reduction of E-selectin, but not VCAM-1, was documented. In addition, anthocyanins and their metabolites significantly decreased (p < 0.001) VEGF production. The present findings suggest that while Peo-3-glc and Pet-3-glc (but not their metabolites) reduced monocyte adhesion to endothelial cells through suppression of E-selectin production, VEGF production was reduced by both anthocyanins and their metabolites, suggesting a role in the regulation of angiogenesis.
Mirko Marino; Cristian Del Bo’; Massimiliano Tucci; Dorothy Klimis-Zacas; Patrizia Riso; Marisa Porrini. Modulation of Adhesion Process, E-Selectin and VEGF Production by Anthocyanins and Their Metabolites in an In Vitro Model of Atherosclerosis. Nutrients 2020, 12, 655 .
AMA StyleMirko Marino, Cristian Del Bo’, Massimiliano Tucci, Dorothy Klimis-Zacas, Patrizia Riso, Marisa Porrini. Modulation of Adhesion Process, E-Selectin and VEGF Production by Anthocyanins and Their Metabolites in an In Vitro Model of Atherosclerosis. Nutrients. 2020; 12 (3):655.
Chicago/Turabian StyleMirko Marino; Cristian Del Bo’; Massimiliano Tucci; Dorothy Klimis-Zacas; Patrizia Riso; Marisa Porrini. 2020. "Modulation of Adhesion Process, E-Selectin and VEGF Production by Anthocyanins and Their Metabolites in an In Vitro Model of Atherosclerosis." Nutrients 12, no. 3: 655.
Background During aging, alterations of the intestinal microbial ecosystem can occur contributing to immunosenescence, inflamm-aging and impairment of intestinal barrier function (increased intestinal permeability; IP). In the context of a diet-microbiota-IP axis in older subjects, food bioactives such as polyphenols may play a beneficial modulatory role. Methods MaPLE is a project centered on a randomized, controlled cross-over dietary intervention trial [polyphenol-rich diet (PR-diet) versus control diet (C-diet)] targeted to older people (≥ 60 y) living in a well-controlled setting (i.e. nursing home). The 8-week interventions are separated by an 8-week wash-out period. Three small portions per day of selected polyphenol-rich foods are consumed during intervention in substitution of other comparable products within the C-diet. Biological samples are collected before and after each treatment period to evaluate markers related to IP, inflammation, vascular function, oxidative stress, gut and blood microbiomics, metabolomics. A sample size of 50 subjects was defined based on IP as primary outcome. Discussion Evidence that increasing the consumption of polyphenol-rich food products can positively affect intestinal microbial ecosystem resulting in reduced IP and decreased translocation of inflammogenic bacterial factors into the bloodstream will be provided. The integration of data from gut and blood microbiomics, metabolomics and other IP-related markers will improve the understanding of the beneficial effect of the intervention in the context of polyphenols−microbiota−IP interactions. Finally, findings obtained will provide a proof of concept of the reliability of the dietary intervention, also contributing to future implementations of dietary guidelines directed to IP management in the older and other at risk subjects. Trial registration The trial is registered at (ISRCTN10214981); April 28, 2017.
Simone Guglielmetti; Stefano Bernardi; Cristian Del Bo’; Antonio Cherubini; Marisa Porrini; Giorgio Gargari; Nicole Hidalgo-Liberona; Raul Gonzalez-Dominguez; Gregorio Peron; Raul Zamora-Ros; Mark S. Winterbone; Benjamin Kirkup; Paul A. Kroon; Cristina Andres-Lacueva; Patrizia Riso. Effect of a polyphenol-rich dietary pattern on intestinal permeability and gut and blood microbiomics in older subjects: study protocol of the MaPLE randomised controlled trial. BMC Geriatrics 2020, 20, 1 -10.
AMA StyleSimone Guglielmetti, Stefano Bernardi, Cristian Del Bo’, Antonio Cherubini, Marisa Porrini, Giorgio Gargari, Nicole Hidalgo-Liberona, Raul Gonzalez-Dominguez, Gregorio Peron, Raul Zamora-Ros, Mark S. Winterbone, Benjamin Kirkup, Paul A. Kroon, Cristina Andres-Lacueva, Patrizia Riso. Effect of a polyphenol-rich dietary pattern on intestinal permeability and gut and blood microbiomics in older subjects: study protocol of the MaPLE randomised controlled trial. BMC Geriatrics. 2020; 20 (1):1-10.
Chicago/Turabian StyleSimone Guglielmetti; Stefano Bernardi; Cristian Del Bo’; Antonio Cherubini; Marisa Porrini; Giorgio Gargari; Nicole Hidalgo-Liberona; Raul Gonzalez-Dominguez; Gregorio Peron; Raul Zamora-Ros; Mark S. Winterbone; Benjamin Kirkup; Paul A. Kroon; Cristina Andres-Lacueva; Patrizia Riso. 2020. "Effect of a polyphenol-rich dietary pattern on intestinal permeability and gut and blood microbiomics in older subjects: study protocol of the MaPLE randomised controlled trial." BMC Geriatrics 20, no. 1: 1-10.
Altered martial indices before orthopedic surgery are associated with higher rates of complications and greatly affect the patient’s functional ability. Oral supplements can optimize the preoperative martial status, with clinical efficacy and the patient’s tolerability being highly dependent on the pharmaceutical formula. Patients undergoing elective hip/knee arthroplasty were randomized to be supplemented with a 30-day oral therapy of sucrosomial ferric pyrophosphate plus L-ascorbic acid. The tolerability was 2.7% among treated patients. Adjustments for confounding factors, such as iron absorption influencers, showed a relevant response limited to older patients (≥ 65 years old), whose uncharacterized Hb loss was averted upon treatment with iron formula. Older patients with no support lost −2.8 ± 5.1%, while the intervention group gained +0.7 ± 4.6% of circulating hemoglobin from baseline (p = 0.019). Gastrointestinal diseases, medications, and possible dietary factors could affect the efficacy of iron supplements. Future opportunities may consider to couple ferric pyrophosphate with other nutrients, to pay attention in avoiding absorption disruptors, or to implement interventions to obtain an earlier martial status optimization at the population level.
Matteo Briguglio; Silvana Hrelia; Marco Malaguti; Elena De Vecchi; Giovanni Lombardi; Giuseppe Banfi; Patrizia Riso; Marisa Porrini; Sergio Romagnoli; Fabio Pino; Tiziano Crespi; Paolo Perazzo. Oral Supplementation with Sucrosomial Ferric Pyrophosphate Plus L-Ascorbic Acid to Ameliorate the Martial Status: A Randomized Controlled Trial. Nutrients 2020, 12, 386 .
AMA StyleMatteo Briguglio, Silvana Hrelia, Marco Malaguti, Elena De Vecchi, Giovanni Lombardi, Giuseppe Banfi, Patrizia Riso, Marisa Porrini, Sergio Romagnoli, Fabio Pino, Tiziano Crespi, Paolo Perazzo. Oral Supplementation with Sucrosomial Ferric Pyrophosphate Plus L-Ascorbic Acid to Ameliorate the Martial Status: A Randomized Controlled Trial. Nutrients. 2020; 12 (2):386.
Chicago/Turabian StyleMatteo Briguglio; Silvana Hrelia; Marco Malaguti; Elena De Vecchi; Giovanni Lombardi; Giuseppe Banfi; Patrizia Riso; Marisa Porrini; Sergio Romagnoli; Fabio Pino; Tiziano Crespi; Paolo Perazzo. 2020. "Oral Supplementation with Sucrosomial Ferric Pyrophosphate Plus L-Ascorbic Acid to Ameliorate the Martial Status: A Randomized Controlled Trial." Nutrients 12, no. 2: 386.
The present study aims to evaluate the ability of peonidin and petunidin-3-glucoside (Peo and Pet-3-glc) and their metabolites (vanillic acid; VA and methyl-gallic acid; MetGA), to prevent monocyte (THP-1) adhesion to endothelial cells (HUVECs), and to reduce the production of VCAM-1, E-selectin and VEGF in a stimulated pro-inflammatory environment, a pivotal step of atherogenesis. Tumor necrosis factor-α (TNF-α; 100 ng mL-1) was used to stimulate the adhesion of labelled monocytes (THP-1) to endothelial cells (HUVECs). Successively, different concentrations of Peo-3-glc and Pet-3-glc (0.02, 0.2, 2 and 20 µM) and VA and MetGA (0.05, 0.5, 5 and 50 µM) were tested. After 24 h, the production of VCAM-1, E-selectin and VEGF was quantified by ELISA kits, while the adhesion process was measured spectrophotometrically. Peo-3-glc and Pet-3-glc (from 0.02 to 20 µM) significantly (p<0.0001) decreased THP-1 adhesion to HUVECs at all concentrations (-37%, -24%, -30% and -47% for Peo-3-glc; -37%, -33%, -33% and -45% for Pet-3-glc). VA, but not MetGA, reduced the adhesion process at 50 µM (-21%; p<0.001). At the same concentrations, a significant (p<0.0001) reduction of E-selectin, but not VCAM-1, was documented. In addition, anthocyanins and their metabolites significantly decreased (p<0.001) VEGF production. The present findings suggest, that while Peo-3-glc and Pet-3-glc, but not their metabolites, reduced monocyte adhesion to endothelial cells through suppression of E-selectin production, VEGF production was reduced by both anthocyanins and their metabolites suggesting a role in regulation of angiogenesis.
Mirko Marino; Cristian Del Bo’; Massimiliano Tucci; Dorothy Klimis-Zacas; Patrizia Riso; Marisa Porrini. Modulation of Adhesion Process, E-Selectin and VEGF Production by Anthocyanins and Their Metabolites in an In-Vitro Model of Atherosclerosis. 2020, 1 .
AMA StyleMirko Marino, Cristian Del Bo’, Massimiliano Tucci, Dorothy Klimis-Zacas, Patrizia Riso, Marisa Porrini. Modulation of Adhesion Process, E-Selectin and VEGF Production by Anthocyanins and Their Metabolites in an In-Vitro Model of Atherosclerosis. . 2020; ():1.
Chicago/Turabian StyleMirko Marino; Cristian Del Bo’; Massimiliano Tucci; Dorothy Klimis-Zacas; Patrizia Riso; Marisa Porrini. 2020. "Modulation of Adhesion Process, E-Selectin and VEGF Production by Anthocyanins and Their Metabolites in an In-Vitro Model of Atherosclerosis." , no. : 1.