Tissue engineering, often referred to as regenerative medicine and reparative medicine, is an interdisciplinary field that necessitates the combined effort of cell biologists, engineers, material scientists, mathematicians, geneticists, and clinicians toward the development of biological substitutes that restore, maintain, or improve tissue function. It has emerged as a rapidly expanding approach to address the organ shortage problem and comprises tissue regeneration and organ substitution. Cells placed on/or within constructs is the most common strategy in tissue engineering. Successful cell seeding depends on fast attachment of cell to scaffolds, high cell survival and uniform cell distribution. The seeding time is strongly dependent on the scaffold material and architecture. Scaffolds provide an initial biochemical substrate for the novel tissue until cells can produce their own extra-cellular matrix (ECM). Thus scaffolds not only define the 3D space for the formation of new tissues, but also serve to provide tissues with appropriate functions. These scaffolds are often critical, both in vivo (within the body) or in vitro (outside the body) mimicking in vivo conditions. Additive fabrication processes represent a new group of non-conventional fabrication techniques recently introduced in the biomedical engineering field. In tissue engineering, additive fabrication processes have been used to produce scaffolds with customised external shape and predefined internal morphology, allowing good control of pore size and pore distribution. This article provides a comprehensive state-of-the-art review of the application of biomanufacturing additive processes in the field of tissue engineering. New and moving trends in biomanufacturing technologies and the concept of direct cell-printing technologies are also discussed.

Tissue engineering represents a new field aiming at developing biological substitutes to restore, maintain, or improve tissue functions. In this approach, scaffolds provide a temporary mechanical and vascular support for tissue regeneration while tissue in-growth is being formed. These scaffolds must be biocompatible, biodegradable, with appropriate porosity, pore structure and distribution, and optimal vascularization with both surface and structural compatibility. The challenge is to establish a proper balance between porosity and mechanical performance of scaffolds. This work investigates the use of two different types of triple periodic minimal surfaces, Schwarz and Schoen, in order to design better biomimetic scaffolds with high surface-to-volume ratio, high porosity and good mechanical properties. The mechanical behaviour of these structures is assessed through the finite element method software Abaqus. The effect of two parametric parameters (thickness and surface radius) is also evaluated regarding its porosity and mechanical behaviour.

Advanced additive techniques are now being developed to fabricate scaffolds with controlled architecture for tissue engineering. These techniques combine computer-aided design (CAD) with computer-aided manufacturing (CAM) tools to produce three-dimensional structures layer by layer in a multitude of materials. Actual prediction of the effective mechanical properties of scaffolds produced by additive technologies, is very important for tissue engineering applications. A novel computer based technique for scaffold design is topological optimisation. Topological optimisation is a form of "shape" optimisation, usually referred to as "layout" optimisation. The goal of topological optimisation is to find the best use of material for a body that is subjected to either a single load or a multiple load distribution. This paper proposes a topological optimisation scheme in order to obtain the ideal topological architectures of scaffolds, maximising its mechanical behaviour.

The purpose of this paper is to extend existing knowledge of 4D printing, in line with Khoo et al. (2015) who defined the production of 4D printing using a single material, and 4D printing of multiple materials. It is proposed that 4D printing can be achieved through the use of functionally graded materials (FGMs) that involve gradational mixing of materials and are produced using an additive manufacturing (AM) technique to achieve a single component. The latest state-of-the-art literature was extensively reviewed, covering aspects of materials, processes, computer-aided design (CAD), applications and made recommendations for future work. This paper clarifies that functionally graded additive manufacturing (FGAM) is defined as a single AM process that includes the gradational mixing of materials to fabricate freeform geometries with variable properties within one component. The paper also covers aspects of materials, processes, CAD, applications and makes recommendations for future work. This paper examines the relationship between FGAM and 4D printing and defines FGAM as a single AM process involving gradational mixing of materials to fabricate freeform geometries with variable properties within one component. FGAM requires better computational tools for modelling, simulation and fabrication because current CAD systems are incapable of supporting the FGAM workflow. It is also identified that other factors, such as strength, type of materials, etc., must be taken into account when selecting an appropriate process for FGAM. More research needs to be conducted on improving the performance of FGAM processes through extensive characterisation of FGMs to generate a comprehensive database and to develop a predictive model for proper process control. It is expected that future work will focus on both material characterisation as well as seamless FGAM control processes. This paper examines the relationship between FGAM and 4D printing and defines FGAM as a single AM process that includes gradational mixing of materials to fabricate freeform geometries with variable properties within one component.

The controlled fabrication of the scaffold structures for tissue engineering is becoming increasingly important as a viable vehicle in future for regenerative medicine. This paper provides a brief description of the conventional techniques used to manufacture scaffolds and the associated limitations, particularly the lack of full control of the pore morphology and architecture as well as reproducibility. Rapid Prototyping and Manufacturing (RP&M) technology, based on layer-by-layer additive approach, offers a unique opportunity to overcome these limitations allowing the build of scaffold structures tailored to specific applications and ultimately individual patients. The main RP&M processes, namely stereolithography; laser sintering; extrusion and Three-Dimensional (3D) printing, are described in detail along the main research efforts deployed towards the fabrication of simple and complex 3D scaffolds.