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My activity is focused on digestive surgery, including hepatobiliary disease, pancreatic diseases, colorectal pathology. The preferred approach is laparoscopic surgery where it is feasible. Also as an Associate Professor, the main activity is with students and residents.
Aim: In our study, we evaluated the prognostic value of four calculated inflammatory ratios in patients with colorectal cancer. Material and methods: A six-year retrospective study was conducted on subjects admitted for colorectal cancer at "Prof. Dr. Octavian Fodor" Regional Institute of Gastroenterology and Hepatology Cluj-Napoca, Romania, from January 2014 until September 2019. The medical charts of patients diagnosed with colorectal cancer were used as the source of raw data and for the calculation of four ratios (neutrophil-to-lymphocyte ratio-NLR, derived neutrophil-to-lymphocyte ratio-dNLR, platelet-to-lymphocyte ratio-PLR, and systemic immune-inflammation index-SII), considered as prognostic markers related to mortality in colorectal cancer. Results: One thousand six hundred and eighty-eight patients, with ages ranging from 17 to 98 years, were evaluated. NLR and dNLR displayed significantly higher values among patients who died (NLR: 4.2 for deceased vs. 3.4 for alive, P-value=0.0224; dNLR: 2.7 for deceased vs. 2.3 for alive, Pvalue= 0.0566). Ischemic cardiomyopathy (odds ratio (OR)=2.70), liver cirrhosis (OR=7.84), postoperative complications (OR=2.39), and neutrophil-to-lymphocyte ratio (OR=1.08) proved to be significant prognostic factors for the primary outcome, independent of age and gender. Conclusions: Patients with high NLR, postoperative complications, ischemic cardiomyopathy an d/or liver cirrhosis are the candidates to a less favorable outcome among subjects with colorectal cancer regardless the age and gender.
Andra Ciocan; Sorana D. Bolboacă; Cristina Drugan; Răzvan A. Ciocan; Florin Graur; Nadim AL Hajjar. The Pattern of Calculated Inflammation Ratios as Prognostic Values in Patients with Colorectal Cancer. Combinatorial Chemistry & High Throughput Screening 2021, 24, 1428 -1435.
AMA StyleAndra Ciocan, Sorana D. Bolboacă, Cristina Drugan, Răzvan A. Ciocan, Florin Graur, Nadim AL Hajjar. The Pattern of Calculated Inflammation Ratios as Prognostic Values in Patients with Colorectal Cancer. Combinatorial Chemistry & High Throughput Screening. 2021; 24 (9):1428-1435.
Chicago/Turabian StyleAndra Ciocan; Sorana D. Bolboacă; Cristina Drugan; Răzvan A. Ciocan; Florin Graur; Nadim AL Hajjar. 2021. "The Pattern of Calculated Inflammation Ratios as Prognostic Values in Patients with Colorectal Cancer." Combinatorial Chemistry & High Throughput Screening 24, no. 9: 1428-1435.
Endorectal ultrasound applications in the evaluation of rectal tumors could be a useful tool in achieving proper staging of rectal cancer. The purpose of this study was to compare the efficacy of rectal tumor staging by flexible endoscopic ultrasound (EUS) with real-time elastography (RTE) using the gold standard post-surgery histological analysis of the resected tissue as the control. The second aim of our research was to establish cutoff values for the EUS-RTE strain ratio corresponding to stages by independently comparing the stiffness values obtained with histology and EUS-RTE staging in order to minimize observation bias. We evaluated the records of 130 patients with a rectal tumor confirmed by biopsy. EUS was used in 70 patients, EUS-RTE—in the other 60. We found no statistically significant differences in staging accuracy when comparing EUS to EUS-RTE. Through a correspondence method between staging assessment and the EUS-RTE stain ratio, we identified cutoff intervals for T2, T3, and T4 staging that were nonoverlapping and proved to be statistically significant in terms of EUS-RTE values (significantly different ascending values from one interval to the other). We found that EUS-RTE offers slightly better, although not statistically significant sensitivity and specificity for T and N stage predictions compared to 2D EUS. Our results showed that EUS-RTE offers slightly higher sensitivity and specificity compared to EUS. Reliable cutoff intervals were found for strain rate elastography, previously available only for shear wave elastography (SWE) which is currently unavailable on any EUS system. Thus, these commonly available EUS-RTE systems can serve as a complementary tool in the staging of rectal tumors.
Adrian Catinean; Gheorghe Balan; Anita Mezei; Emil-Claudiu Botan; Andrei-Otto Mitre; Radu Motocu; Florin Graur; Dan-Tudor Eniu; Maria-Adriana Neag. Endoscopic Ultrasound Elastography in the Assessment of Rectal Tumors: How Well Does It Work in Clinical Practice? Diagnostics 2021, 11, 1180 .
AMA StyleAdrian Catinean, Gheorghe Balan, Anita Mezei, Emil-Claudiu Botan, Andrei-Otto Mitre, Radu Motocu, Florin Graur, Dan-Tudor Eniu, Maria-Adriana Neag. Endoscopic Ultrasound Elastography in the Assessment of Rectal Tumors: How Well Does It Work in Clinical Practice? Diagnostics. 2021; 11 (7):1180.
Chicago/Turabian StyleAdrian Catinean; Gheorghe Balan; Anita Mezei; Emil-Claudiu Botan; Andrei-Otto Mitre; Radu Motocu; Florin Graur; Dan-Tudor Eniu; Maria-Adriana Neag. 2021. "Endoscopic Ultrasound Elastography in the Assessment of Rectal Tumors: How Well Does It Work in Clinical Practice?" Diagnostics 11, no. 7: 1180.
Contamination of surface waters with pathogens as well as all diseases associated with such events are a significant concern worldwide. In recent decades, there has been a growing interest in developing analytical methods with good performance for the detection of this category of contaminants. The most important analytical methods applied for the determination of bacteria in waters are traditional ones (such as bacterial culturing methods, enzyme-linked immunoassay, polymerase chain reaction, and loop-mediated isothermal amplification) and advanced alternative methods (such as spectrometry, chromatography, capillary electrophoresis, surface-enhanced Raman scattering, and magnetic field-assisted and hyphenated techniques). In addition, optical and electrochemical sensors have gained much attention as essential alternatives for the conventional detection of bacteria. The large number of available methods have been materialized by many publications in this field aimed to ensure the control of water quality in water resources. This study represents a critical synthesis of the literature regarding the latest analytical methods covering comparative aspects of pathogen contamination of water resources. All these aspects are presented as representative examples, focusing on two important bacteria with essential implications on the health of the population, namely Pseudomonas aeruginosa and Escherichia coli.
Alexandra Canciu; Mihaela Tertis; Oana Hosu; Andreea Cernat; Cecilia Cristea; Florin Graur. Modern Analytical Techniques for Detection of Bacteria in Surface and Wastewaters. Sustainability 2021, 13, 7229 .
AMA StyleAlexandra Canciu, Mihaela Tertis, Oana Hosu, Andreea Cernat, Cecilia Cristea, Florin Graur. Modern Analytical Techniques for Detection of Bacteria in Surface and Wastewaters. Sustainability. 2021; 13 (13):7229.
Chicago/Turabian StyleAlexandra Canciu; Mihaela Tertis; Oana Hosu; Andreea Cernat; Cecilia Cristea; Florin Graur. 2021. "Modern Analytical Techniques for Detection of Bacteria in Surface and Wastewaters." Sustainability 13, no. 13: 7229.
Aloe, the largest genus in the Asphodelaceae family, comprises 548 species, with A. vera, A. arborescens and A. ferox being among the most widely studied species. Aloe species originated in arid climates and cover various habitats, from sea level up to 2700 m, and from desert to closed-canopy forests. For human health, Aloe species are the richest natural sources. The biological activity of Aloe sp. constituents covers a wide spectrum. Most of the indications come from traditional, folkloric use and several have been verified by in vitro or in vivo studies. Emodin, the main phenolic component, has showed anti-neoplastic, anti-inflammatory, anti-angiogenic and toxicological potential for use in pharmacology. Polysaccharides, with acemannan being the most important, are present in high abundance in Aloe gels. Acemannan has been reported to have applications in oral, metabolic and cardiovascular diseases, oncology, dentistry and wound healing. The effectiveness of Aloe sp. constituents on colon, liver, duodenum, skin, pancreas, intestine, lungs and kidneys cancers was highly studied with remarkable findings. Regarding the metabolic syndrome, Aloe sp. can be used as an antidiabetic and reduces cholesterol and total body fat. Constituents of Aloe sp. are nontoxic in experimental acute oral studies and are widely used in cosmetology and as bitter agents or consistence modifiers in food and beverages. Traditional Aloe remedies cover most human diseases; however, in order to gain legitimacy, the Aloe-derived drugs must have a well-established composition, with thoroughly investigated adverse effects and conventional drug interactions.
Aida Puia; Cosmin Puia; Emil Moiș; Florin Graur; Alin Fetti; Mira Florea. The phytochemical constituents and therapeutic uses of genus Aloe: A review. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 2021, 49, 12332 -12332.
AMA StyleAida Puia, Cosmin Puia, Emil Moiș, Florin Graur, Alin Fetti, Mira Florea. The phytochemical constituents and therapeutic uses of genus Aloe: A review. Notulae Botanicae Horti Agrobotanici Cluj-Napoca. 2021; 49 (2):12332-12332.
Chicago/Turabian StyleAida Puia; Cosmin Puia; Emil Moiș; Florin Graur; Alin Fetti; Mira Florea. 2021. "The phytochemical constituents and therapeutic uses of genus Aloe: A review." Notulae Botanicae Horti Agrobotanici Cluj-Napoca 49, no. 2: 12332-12332.
Metabolomics coupled with bioinformatics may identify relevant biomolecules such as putative biomarkers of specific metabolic pathways related to colorectal diagnosis, classification and prognosis. This study performed an integrated metabolomic profiling of blood serum from 25 colorectal cancer (CRC) cases previously classified (Stage I to IV) compared with 16 controls (disease-free, non-CRC patients), using high-performance liquid chromatography and mass spectrometry (UPLC-QTOF-ESI+ MS). More than 400 metabolites were separated and identified, then all data were processed by the advanced Metaboanalyst 5.0 online software, using multi- and univariate analysis, including specificity/sensitivity relationships (area under the curve (AUC) values), enrichment and pathway analysis, identifying the specific pathways affected by cancer progression in the different stages. Several sub-classes of lipids including phosphatidylglycerols (phosphatidylcholines (PCs), phosphatidylethanolamines (PEs) and PAs), fatty acids and sterol esters as well as ceramides confirmed the “lipogenic phenotype” specific to CRC development, namely the upregulated lipogenesis associated with tumor progression. Both multivariate and univariate bioinformatics confirmed the relevance of some putative lipid biomarkers to be responsible for the altered metabolic pathways in colorectal cancer.
Claudiu Răchieriu; Dan Eniu; Emil Moiş; Florin Graur; Carmen Socaciu; Mihai Socaciu; Nadim Hajjar. Lipidomic Signatures for Colorectal Cancer Diagnosis and Progression Using UPLC-QTOF-ESI+MS. Biomolecules 2021, 11, 417 .
AMA StyleClaudiu Răchieriu, Dan Eniu, Emil Moiş, Florin Graur, Carmen Socaciu, Mihai Socaciu, Nadim Hajjar. Lipidomic Signatures for Colorectal Cancer Diagnosis and Progression Using UPLC-QTOF-ESI+MS. Biomolecules. 2021; 11 (3):417.
Chicago/Turabian StyleClaudiu Răchieriu; Dan Eniu; Emil Moiş; Florin Graur; Carmen Socaciu; Mihai Socaciu; Nadim Hajjar. 2021. "Lipidomic Signatures for Colorectal Cancer Diagnosis and Progression Using UPLC-QTOF-ESI+MS." Biomolecules 11, no. 3: 417.
The stability of receiver operating characteristic in context of random split used in development and validation sets, as compared to the full models for three inflammatory ratios (neutrophil-to-lymphocyte (NLR), derived neutrophil-to-lymphocyte (dNLR) and platelet-to-lymphocyte (PLR) ratio) evaluated as predictors for metastasis in patients with colorectal cancer, was investigated. Data belonging to patients admitted with the diagnosis of colorectal cancer from January 2014 until September 2019 in a single hospital were used. There were 1688 patients eligible for the study, 418 in the metastatic stage. All investigated inflammatory ratios proved to be significant classification models on both the full models and on cross-validations (AUCs > 0.05). High variability of the cut-off values was observed in the unrestricted and restricted split (full models: 4.255 for NLR, 2.745 for dNLR and 255.56 for PLR; random splits: cut-off from 3.215 to 5.905 for NLR, from 2.625 to 3.575 for dNLR and from 134.67 to 335.9 for PLR), but with no effect on the models characteristics or performances. The investigated biomarkes proved limited value as predictors for metastasis (AUCs < 0.8), with largely sensitivity and specificity (from 33.3% to 79.2% for the full model and 29.1% to 82.7% in the restricted splits). Our results showed that a simple random split of observations, weighting or not the patients with and whithout metastasis, in a ROC analysis assures the performances similar to the full model, if at least 70% of the available population is included in the study.
Andra Ciocan; Nadim Al Hajjar; Florin Graur; Valentin C. Oprea; Răzvan A. Ciocan; Sorana D. Bolboacă. Receiver Operating Characteristic Prediction for Classification: Performances in Cross-Validation by Example. Mathematics 2020, 8, 1741 .
AMA StyleAndra Ciocan, Nadim Al Hajjar, Florin Graur, Valentin C. Oprea, Răzvan A. Ciocan, Sorana D. Bolboacă. Receiver Operating Characteristic Prediction for Classification: Performances in Cross-Validation by Example. Mathematics. 2020; 8 (10):1741.
Chicago/Turabian StyleAndra Ciocan; Nadim Al Hajjar; Florin Graur; Valentin C. Oprea; Răzvan A. Ciocan; Sorana D. Bolboacă. 2020. "Receiver Operating Characteristic Prediction for Classification: Performances in Cross-Validation by Example." Mathematics 8, no. 10: 1741.
Introduction: Robot-assisted liver tumor ablation has emerged as a new minimally invasive therapeutic strategy for hepatocellular carcinoma, as well as colorectal metastases with higher accuracy, in a smaller time span and with a lower radiation dose than in the manual approach. Several ablation methods count, mostly used are radiofrequency ablation, microwave ablation and cryoablation. Materials and methods: The specialty literature was surveyed in order to retrieve manuscripts reporting “robot-assisted”, “liver tumor”, “ablation”. The search strategy was applied in three different databases, Scopus, Web of Science and Embase. Fifteen original articles were selected and seven were excluded from the study, in order to compare results of robotic ablation for liver tumors both on patient series or on experimental models. Results: Out of a total of fifteen articles included in the study, six articles focused on the clinical aspect of robotic liver tumor aspect, providing patients description and characteristics on a total of 172 subjects. There were nine studies, which focused on the technical assessment of the robot during ablation on experimental model. The indications for percutaneous ablation usually include malignant tumors, hepatocellular carcinoma (55 tumors) or colorectal liver metastases (92 tumors) for lesions smaller than 3 cm in diameter, which cannot benefit from liver resection. The most commonly reported type of ablation was microwave technique (43%), followed by radiofrequency (40%). Only one article evaluated laser ablation effects and another one reported irreversible electroporation. The number of needles used for ablation according to the tumor volume varied from 1 in 50% of cases to 5 and the number of needle readjustments reached 1 to 14. The type of robot used was different in the fifteen studies. Conclusions: Development of more versatile robots and intuitive software that will reduce the total time of the procedures is highly expected, thus transforming imaging guided robotic ablation into the golden standard procedure for inoperable primary hepatic tumors or liver metastases.
Andra Ciocan; Radu Elisei; Florin Graur; Emil I. Mois; Sorana D. Bolboaca; Corina Radu; Calin Vaida; Nadim Al Hajjar. Robot-Assisted Ablation of Liver Hepatocellular Carcinoma and Colorectal Metastases: A Systematic Review. Mechanical Engineering and Materials 2020, 206 -218.
AMA StyleAndra Ciocan, Radu Elisei, Florin Graur, Emil I. Mois, Sorana D. Bolboaca, Corina Radu, Calin Vaida, Nadim Al Hajjar. Robot-Assisted Ablation of Liver Hepatocellular Carcinoma and Colorectal Metastases: A Systematic Review. Mechanical Engineering and Materials. 2020; ():206-218.
Chicago/Turabian StyleAndra Ciocan; Radu Elisei; Florin Graur; Emil I. Mois; Sorana D. Bolboaca; Corina Radu; Calin Vaida; Nadim Al Hajjar. 2020. "Robot-Assisted Ablation of Liver Hepatocellular Carcinoma and Colorectal Metastases: A Systematic Review." Mechanical Engineering and Materials , no. : 206-218.
Locally advanced rectal cancer (LARC) response to neoadjuvant chemoradiotherapy (nCRT) is very heterogeneous and up to 30% of patients are considered non-responders, presenting no tumor regression after nCRT. This study aimed to determine the ability of pre-treatment T2-weighted based radiomics features to predict LARC non-responders. A total of 67 LARC patients who underwent a pre-treatment MRI followed by nCRT and total mesorectal excision were assigned into training (n = 44) and validation (n = 23) groups. In both datasets, the patients were categorized according to the Ryan tumor regression grade (TRG) system into non-responders (TRG = 3) and responders (TRG 1 and 2). We extracted 960 radiomic features/patient from pre-treatment T2-weighted images. After a three-step feature selection process, including LASSO regression analysis, we built a radiomics score with seven radiomics features. This score was significantly higher among non-responders in both training and validation sets (p < 0.001 and p = 0.03) and it showed good predictive performance for LARC non-response, achieving an area under the curve (AUC) = 0.94 (95% CI: 0.82–0.99) in the training set and AUC = 0.80 (95% CI: 0.58–0.94) in the validation group. The multivariate analysis identified the radiomics score as an independent predictor for the tumor non-response (OR = 6.52, 95% CI: 1.87–22.72). Our results indicate that MRI radiomics features could be considered as potential imaging biomarkers for early prediction of LARC non-response to neoadjuvant treatment.
Bianca Petresc; Andrei Lebovici; Cosmin Caraiani; Diana Sorina Feier; Florin Graur; Mircea Marian Buruian. Pre-Treatment T2-WI Based Radiomics Features for Prediction of Locally Advanced Rectal Cancer Non-Response to Neoadjuvant Chemoradiotherapy: A Preliminary Study. Cancers 2020, 12, 1894 .
AMA StyleBianca Petresc, Andrei Lebovici, Cosmin Caraiani, Diana Sorina Feier, Florin Graur, Mircea Marian Buruian. Pre-Treatment T2-WI Based Radiomics Features for Prediction of Locally Advanced Rectal Cancer Non-Response to Neoadjuvant Chemoradiotherapy: A Preliminary Study. Cancers. 2020; 12 (7):1894.
Chicago/Turabian StyleBianca Petresc; Andrei Lebovici; Cosmin Caraiani; Diana Sorina Feier; Florin Graur; Mircea Marian Buruian. 2020. "Pre-Treatment T2-WI Based Radiomics Features for Prediction of Locally Advanced Rectal Cancer Non-Response to Neoadjuvant Chemoradiotherapy: A Preliminary Study." Cancers 12, no. 7: 1894.
The development of an esophagorespiratory fistula (ERF) in patients with esophageal cancer (EC) is associated with poor prognosis. We observed a high rate of vocal fold paralysis (VFP) in patients with ERF. Data on prevalence and complications of VFP in ERF are lacking. The present study investigated the incidence of VFP in patients with malignant ERF and examined possible risk factors and the impact on survival. We performed a retrospective case-control study of 46 institutional cases of EC patients with ERF in a time period of eleven years. Patients were matched to 92 randomly selected controls (EC patients without ERF) in a 1:2 fashion for tumor localization and histology. Demographics, clinical characteristics, recurrence, treatment modalities as well as survival were analyzed. Esophageal cancer patients with ERF developed more often VFP than EC patients without ERF (59% vs. 21%; p=0.02; odds ratio (OR) 4.9). Esophageal cancer patients with ERF had a more pronounced weight loss (7.1 vs. 11.5 kg; P = 0.008), as well as higher rates of esophageal (p=<0.001; OR 22.9) and tracheal stenting (p=<0.001; OR 76.8). Proximal tumor growth (p=0.004; OR 7.9), fistula formation to the trachea (p=<0.001; OR 17.2) and recurrent disease (p=0.04, OR 4.7) was associated with VFP development in EC patients with ERF. Vocal fold paralysis in ERF did not adversely affect five-year survival. Vocal fold paralysis is a common complication in more than half of the patients with ERF in EC. It is associated with proximal tumor growth, fistula formation to the trachea and disease recurrence, but does not influence survival.
Claudia Gabriela Moldovanu; Cosmin Caraiani; Bogdan Furnea; Florin Graur. A Rare Case of Aeroportia Due to Acute Gastric Dilatation - when the Radiologist Goes beyond the Imagess. Journal of Gastrointestinal and Liver Diseases 2020, 29, 3 -3.
AMA StyleClaudia Gabriela Moldovanu, Cosmin Caraiani, Bogdan Furnea, Florin Graur. A Rare Case of Aeroportia Due to Acute Gastric Dilatation - when the Radiologist Goes beyond the Imagess. Journal of Gastrointestinal and Liver Diseases. 2020; 29 (1):3-3.
Chicago/Turabian StyleClaudia Gabriela Moldovanu; Cosmin Caraiani; Bogdan Furnea; Florin Graur. 2020. "A Rare Case of Aeroportia Due to Acute Gastric Dilatation - when the Radiologist Goes beyond the Imagess." Journal of Gastrointestinal and Liver Diseases 29, no. 1: 3-3.
The rapid detection of bacterial strains has become a major topic thoroughly discussed across the biomedical field. Paired with the existence of nosocomial pathogen agents that imply extreme medical and financial challenges throughout diagnosis and treatment, the development of rapid and easy-to-use sensing devices has gained an increased amount of attention. Moreover, antibiotic resistance considered by World Health Organization as one of the “biggest threats to global health, food security, and development today” enables this topic as high priority. Pseudomonas aeruginosa, one of the most ubiquitous bacterial strains, has various quorum-sensing systems that are a direct cause of their virulence. One of them is represented by pyocyanin, a blue pigment with electroactive properties that is synthesized from early stages of bacterial colonization. Thus, the sensitive detection of this biomarker could enable a personalized and efficient therapy. It was achieved with the development of an electrochemical sensor based on a thermosensitive polymer, modified with Au/Ag nanoalloy for the rapid and accurate detection of pyocyanin, a virulence biomarker of Pseudomonas aeruginosa. The sensor displayed a linear range from 0.12 to 25 μM, and a limit of detection of 0.04 μM (signal/noise = 3). It was successfully tested in real samples spiked with the target analyte without any pretreatment other than a dilution step. The detection of pyocyanin with high recovery in whole blood in a time frame of 5–10 min from the moment of collection was performed with this electrochemical sensor.
Andreea Cernat; Alexandra Canciu; Mihaela Tertis; Florin Graur; Cecilia Cristea. Synergic action of thermosensitive hydrogel and Au/Ag nanoalloy for sensitive and selective detection of pyocyanin. Analytical and Bioanalytical Chemistry 2019, 411, 3829 -3838.
AMA StyleAndreea Cernat, Alexandra Canciu, Mihaela Tertis, Florin Graur, Cecilia Cristea. Synergic action of thermosensitive hydrogel and Au/Ag nanoalloy for sensitive and selective detection of pyocyanin. Analytical and Bioanalytical Chemistry. 2019; 411 (17):3829-3838.
Chicago/Turabian StyleAndreea Cernat; Alexandra Canciu; Mihaela Tertis; Florin Graur; Cecilia Cristea. 2019. "Synergic action of thermosensitive hydrogel and Au/Ag nanoalloy for sensitive and selective detection of pyocyanin." Analytical and Bioanalytical Chemistry 411, no. 17: 3829-3838.
Despite the fact that cancer research has experienced important advances and remarkable improvement in the curing processes during the last decades, this disease still occupies a leading position among the causes of death worldwide. It has been demonstrated that there is an interconnection between the overexpression of interleukin-6 cytokine and the tumor growth, metastasis, and therapeutic resistance in several types of malignancies. Herein, a highly sensitive and selective aptasensor for quantitative detection of interleukin-6 was developed by using a glassy carbon electrode modified with p-aminobenzoic acid, p-aminothiophenol and gold nanoparticles. A thio-terminated aptamer specific for interleukin-6 was immobilized on the surface of the modified electrode via the formation of gold-sulfur bonds. This DNA oligonucleotide was then used as a detection probe to capture the target protein at the biosensor surface allowing label-free detection by electrochemical impedance spectroscopy. The developed aptasensor showed a good linear response from 5 pgmL−1 to 100 ngmL−1 with a detection limit of 1.6 pgmL−1, within the range of physiological concentration of the protein. The biosensor exhibited high selectivity and has been successfully used to detect interleukin-6 in blood samples collected from patients suffering of colorectal cancer, with excellent recoveries after the addition of known amount of the target protein.
Mihaela Tertis; Petrica Ionut Leva; Diana Bogdan; Maria Suciu; Florin Graur; Cecilia Cristea. Impedimetric aptasensor for the label-free and selective detection of Interleukin-6 for colorectal cancer screening. Biosensors and Bioelectronics 2019, 137, 123 -132.
AMA StyleMihaela Tertis, Petrica Ionut Leva, Diana Bogdan, Maria Suciu, Florin Graur, Cecilia Cristea. Impedimetric aptasensor for the label-free and selective detection of Interleukin-6 for colorectal cancer screening. Biosensors and Bioelectronics. 2019; 137 ():123-132.
Chicago/Turabian StyleMihaela Tertis; Petrica Ionut Leva; Diana Bogdan; Maria Suciu; Florin Graur; Cecilia Cristea. 2019. "Impedimetric aptasensor for the label-free and selective detection of Interleukin-6 for colorectal cancer screening." Biosensors and Bioelectronics 137, no. : 123-132.
A novel, simple and versatile nanocomposite platform based on graphene-oxide/chitosan modified screen printed electrode was optimized and used for the development of an immunosensor for the sensitive and highly selective electrochemical detection of serotonin. Graphene oxide has been used for providing carboxylic groups which form covalent bonds with the terminal amine groups from the antibody units, thus ensuring the immobilization of the biocomponent at the electrode surface. The presence of chitosan is necessary to ensure the stability of the nanomaterial film deposited on the electrode’ surface during the modification steps. The platform development protocol was optimized throughout the study by using electrochemical methods (electrochemical impedance spectroscopy, cyclic voltammetry and differential pulse voltammetry) as well as scanning electron microscopy. The following steps, namely immobilization of the antibody, blocking of the active free sites from graphene template with bovine serum albumin and testing of the immunosensor after immobilization of the analyte molecules at the surface, were optimized based on the differential pulse voltammetry tests following the signal obtained for the electrochemical oxidation of serotonin. The newly customized sensor was found to have a detection range from 10 nM to 100 μM and a limit of detection of 3.2 nM, being used for serotonin detection from human serum, saliva, artificial tears and urine samples as well as in the presence of different interferences, when good recovery rates were obtained.
Alina Adumitrăchioaie; Mihaela Tertiș; Maria Suciu; Florin Graur; Cecilia Cristea. A novel immunosensing platform for serotonin detection in complex real samples based on graphene oxide and chitosan. Electrochimica Acta 2019, 311, 50 -61.
AMA StyleAlina Adumitrăchioaie, Mihaela Tertiș, Maria Suciu, Florin Graur, Cecilia Cristea. A novel immunosensing platform for serotonin detection in complex real samples based on graphene oxide and chitosan. Electrochimica Acta. 2019; 311 ():50-61.
Chicago/Turabian StyleAlina Adumitrăchioaie; Mihaela Tertiș; Maria Suciu; Florin Graur; Cecilia Cristea. 2019. "A novel immunosensing platform for serotonin detection in complex real samples based on graphene oxide and chitosan." Electrochimica Acta 311, no. : 50-61.
The evaluation of patients with early hepatocellular carcinoma (HCC) referred for liver resection is still a matter of debate. Aims: 1) to compare liver stiffness measurement (LSM) by transient elastography with hepatic venous pressure gradient (HVPG) in the prediction of decompensation after liver resection in patients with cirrhosis and early HCC; 2) to identify which definition for posthepatectomy liver failure is better associated with survival. Fifty-one patients (MELD score of 10±3) were included. In this group, 34 patients underwent HVPG measurement, of which 13 (38%) had clinically significant portal hypertension (CSPH) and 35 patients underwent LSM (21.8±17.9 kPa). The study's end-points were: posthepatectomy liver failure (PHLF) defined according to International Study Group of Liver Surgery criteria and 3-month decompensation defined as de novo ascites, variceal bleeding, jaundice, hepatic encephalopathy and acute kidney injury. The performance of LSM compared to HVPG in predicting the end-points were assessed by AUROC curves and accuracy. Twenty (39%) patients developed PHLF and 15 (29%) developed decompensationat 3 months. Three-month decompensation tended to be better correlated with survival. LSM performed well in predicting decompensation at 3 months (AUROC=0.78, 95%CI: 0.63-0.94; p=0.01), comparable with HVPG (AUROC=0.89, 95%CI: 0.79-1.00; p<0.01) (DeLong test p=0.21). LSM was not sufficiently accurate to predict PHLF. LSM has a similar performance to HVPG in predicting decompensation at 3 months in patients with early HCC submitted to liver resection. Three-month decompensation is better associated with survival.
Bogdan Procopet; Petra Fischer; Adelina Horhat; Emil Mois; Horia Stefanescu; Mihai Comsa; Florin Graur; Adrian Bartos; Monica Lupsor-Platon; Radu Badea; Mircea Grigorescu; Marcel Tantau; Zeno Sparchez; Nadim Al Hajjar. Good performance of liver stiffness measurement in the prediction of postoperative hepatic decompensation in patients with cirrhosis complicated with hepatocellular carcinoma. Medical Ultrasonography 2018, 20, 272 -277.
AMA StyleBogdan Procopet, Petra Fischer, Adelina Horhat, Emil Mois, Horia Stefanescu, Mihai Comsa, Florin Graur, Adrian Bartos, Monica Lupsor-Platon, Radu Badea, Mircea Grigorescu, Marcel Tantau, Zeno Sparchez, Nadim Al Hajjar. Good performance of liver stiffness measurement in the prediction of postoperative hepatic decompensation in patients with cirrhosis complicated with hepatocellular carcinoma. Medical Ultrasonography. 2018; 20 (3):272-277.
Chicago/Turabian StyleBogdan Procopet; Petra Fischer; Adelina Horhat; Emil Mois; Horia Stefanescu; Mihai Comsa; Florin Graur; Adrian Bartos; Monica Lupsor-Platon; Radu Badea; Mircea Grigorescu; Marcel Tantau; Zeno Sparchez; Nadim Al Hajjar. 2018. "Good performance of liver stiffness measurement in the prediction of postoperative hepatic decompensation in patients with cirrhosis complicated with hepatocellular carcinoma." Medical Ultrasonography 20, no. 3: 272-277.
P-POSSUM represents a useful tool for appreciating the complication and death risk after PD, but better results could be obtain by considering also specific PD risk factors.
Raluca Bodea; Nadim Al Hajjar; Adrian Bartos; Florin Zaharie; Florin Graur; Cornel Iancu. Evaluation of P-POSSUM Risk Scoring System in Prediction of Morbidity and Mortality after Pancreaticoduodenectomy. Chirurgia 2018, 113, 399 -404.
AMA StyleRaluca Bodea, Nadim Al Hajjar, Adrian Bartos, Florin Zaharie, Florin Graur, Cornel Iancu. Evaluation of P-POSSUM Risk Scoring System in Prediction of Morbidity and Mortality after Pancreaticoduodenectomy. Chirurgia. 2018; 113 (3):399-404.
Chicago/Turabian StyleRaluca Bodea; Nadim Al Hajjar; Adrian Bartos; Florin Zaharie; Florin Graur; Cornel Iancu. 2018. "Evaluation of P-POSSUM Risk Scoring System in Prediction of Morbidity and Mortality after Pancreaticoduodenectomy." Chirurgia 113, no. 3: 399-404.
National databases for pancreaticoduodenectomies (PD) have contributed to better postoperative outcomes after such complex surgical procedure because the multicentre collection of data allowed more reliable analyses with quality assessment and further improvement of technical issues and perioperative management. The current practice and outcomes after PD are poorly known in Romania because there was no national database for these patients. Thus, in 2016 a national-intent electronic registry for PD was proposed for all Romanian surgical centers. The study aims to present the preliminary results of this national-intent registry for PD after one-year enrollment. Patients The database was started on October 1st, 2016. Data were prospectively collected with an electronic online form including 102 items for each patient. The registry was opened to all the Departments of Surgery from Romania performing PD, with no restriction. During the first year of enrollment were collected the data of 181 patients with PD performed by 24 surgeons from four surgical centers. The age of patients was 64 years (28 - 81 years), with slightly male predominance (61.3%). Computed tomography was the main preoperative imaging investigation (84.5%). All the PDs were performed by an open approach. The Whipple technique was used in 53% of patients, and a venous resection was required in 14.3% of cases. A posterior approach PD was considered in 16.6% of patients. The stomach was used to treat the distal remnant pancreas in 50.1% of patients. The operative time was 285 min (110 - 615 min), and the estimated blood loss was 400 ml (80 - 3000 ml). The overall morbidity rate was 55.8%, with severe (i.e., grade III-IV Dindo-Clavien) morbidity rate of 10%, and 3.9% in-hospital mortality rate. The overall pancreatic fistula, delayed gastric emptying and hemorrhage rates were 19.9%, 39.8% and 15.5%. Periampullary malignancies were the main indications for PD (78.9%), with pancreatic cancer on the top (48%). To build a prospective electronic online database for PD in Romania appears to be a feasible project and a useful tool to know the current practice and outcomes after PD in our country. However, improvements are still required to encourage a larger number of surgical centers to introduce the data of patients with PD.
Adrian Bartos; Mihnea Ionescu; Cornel Iancu; Cezar Stroescu; Florin Zaharie; Vladislav Brasoveanu; Nadim Al Hajjar; Catalin Vasilescu; Florin Graur; Ionut Hutanu; Lucian Mocan; Leonard David; Raluca Bodea; Dan Cacovean; Geza Molnar; Luminita Furcea; Sorin Alexandrescu; Emil Matei; Gabriel Mitulescu; Constantin Ungureanu; Aurel Tonea; Radu Zamfir; Irinel Popescu; Traian Dumitrascu. An Attempt to Build a National Prospective Electronic Database for Pancreaticoduodenectomies in Romania - Preliminary Results of the First Year Enrollment. Chirurgia 2018, 113, 374 -384.
AMA StyleAdrian Bartos, Mihnea Ionescu, Cornel Iancu, Cezar Stroescu, Florin Zaharie, Vladislav Brasoveanu, Nadim Al Hajjar, Catalin Vasilescu, Florin Graur, Ionut Hutanu, Lucian Mocan, Leonard David, Raluca Bodea, Dan Cacovean, Geza Molnar, Luminita Furcea, Sorin Alexandrescu, Emil Matei, Gabriel Mitulescu, Constantin Ungureanu, Aurel Tonea, Radu Zamfir, Irinel Popescu, Traian Dumitrascu. An Attempt to Build a National Prospective Electronic Database for Pancreaticoduodenectomies in Romania - Preliminary Results of the First Year Enrollment. Chirurgia. 2018; 113 (3):374-384.
Chicago/Turabian StyleAdrian Bartos; Mihnea Ionescu; Cornel Iancu; Cezar Stroescu; Florin Zaharie; Vladislav Brasoveanu; Nadim Al Hajjar; Catalin Vasilescu; Florin Graur; Ionut Hutanu; Lucian Mocan; Leonard David; Raluca Bodea; Dan Cacovean; Geza Molnar; Luminita Furcea; Sorin Alexandrescu; Emil Matei; Gabriel Mitulescu; Constantin Ungureanu; Aurel Tonea; Radu Zamfir; Irinel Popescu; Traian Dumitrascu. 2018. "An Attempt to Build a National Prospective Electronic Database for Pancreaticoduodenectomies in Romania - Preliminary Results of the First Year Enrollment." Chirurgia 113, no. 3: 374-384.
Luminița Furcea; Emil Mois; Nadim Al Hajjar; Andrada Seicean; Radu Badea; Florin Graur. Pancreatic Gangliocytic Paraganglioma – CEUS Appearance. Journal of Gastrointestinal and Liver Diseases 2017, 26, 336 .
AMA StyleLuminița Furcea, Emil Mois, Nadim Al Hajjar, Andrada Seicean, Radu Badea, Florin Graur. Pancreatic Gangliocytic Paraganglioma – CEUS Appearance. Journal of Gastrointestinal and Liver Diseases. 2017; 26 (4):336.
Chicago/Turabian StyleLuminița Furcea; Emil Mois; Nadim Al Hajjar; Andrada Seicean; Radu Badea; Florin Graur. 2017. "Pancreatic Gangliocytic Paraganglioma – CEUS Appearance." Journal of Gastrointestinal and Liver Diseases 26, no. 4: 336.
Robotic surgery is in continuous development proving to be not only a better therapeutic option in certain procedures but also a pioneer field in which research occupies a very important role. The history of robotic begins with science-fiction literature, but it profiles the industrial and also health robots that are nowadays used. Robotic surgery begins later on with modified industrial robots, but from that point it has its own development. This article is a short history of surgical robotics, continued with the presentation of some surgical robots currently used. At the end, the characteristics of the future surgical robot are discussed, as well a proposal for a minimally invasive SILS robot.
F. Graur; E. Radu; N. Al Hajjar; C. Vaida; D. Pisla. Surgical Robotics—Past, Present and Future. Mechanical Engineering and Materials 2017, 159 -171.
AMA StyleF. Graur, E. Radu, N. Al Hajjar, C. Vaida, D. Pisla. Surgical Robotics—Past, Present and Future. Mechanical Engineering and Materials. 2017; ():159-171.
Chicago/Turabian StyleF. Graur; E. Radu; N. Al Hajjar; C. Vaida; D. Pisla. 2017. "Surgical Robotics—Past, Present and Future." Mechanical Engineering and Materials , no. : 159-171.
A. Calborean; Florin Graur; Vasile Bintintan. The influence of correlation effects on the electronic structure of double-decker bis(phthalocyaninato)-Dy, Tb complexes. Computational and Theoretical Chemistry 2017, 1112, 104 -110.
AMA StyleA. Calborean, Florin Graur, Vasile Bintintan. The influence of correlation effects on the electronic structure of double-decker bis(phthalocyaninato)-Dy, Tb complexes. Computational and Theoretical Chemistry. 2017; 1112 ():104-110.
Chicago/Turabian StyleA. Calborean; Florin Graur; Vasile Bintintan. 2017. "The influence of correlation effects on the electronic structure of double-decker bis(phthalocyaninato)-Dy, Tb complexes." Computational and Theoretical Chemistry 1112, no. : 104-110.
Correlations between DFT and experimental measurements on Zn(2+) and Ni(2+) chiral meso-(α,β-unsaturated)- porphyrins were performed using Kohn-Sham methodology. The exchange-correlation Becke88-Perdew86 functional was used in conjunction with double-zeta Slater basis sets. An accurate description of the electronic processes depending on the metal ion (Zn, Ni) or ligand (perilaldehyde and myrtenal) was made, confirming experimental results in terms of structural and electronic modifications. Moreover, this theoretical study provides a stronger knowledge and interpretation of the dynamical conformational features of the free base, Zn and Ni structures. Fundamental links between the central metallic atom and distortions of the porphyrinic core and ligands were demonstrated, in agreement with experimental data. We observed that the core in ZnPeriP and ZnMyrtP species is almost flat, in comparison with the Ni porphyrinic core, which appeared much more distorted. The type of distortion differs between PeriP and MyrtP ligands, with a combined saddled-ruffled characteristic with the former and a pronounced ruffled twisting for the latter. Finally, conformational energy barriers were extracted by spinning one of the arms in steps of 20° in a 360° dihedral angle. The resulted conformational barriers for NiPeriP or NiMyrtP are lower in energy than for ZnPeriP or ZnMyrtP, in agreement with experimental data.
Adrian Calborean; Florin Graur; Vasile Bintintan. DFT computational correlations on conformational barriers of Zn2+ and Ni2+ chiral meso-(?,?-unsaturated)- porphyrins. Journal of Molecular Modeling 2017, 23, 7 .
AMA StyleAdrian Calborean, Florin Graur, Vasile Bintintan. DFT computational correlations on conformational barriers of Zn2+ and Ni2+ chiral meso-(?,?-unsaturated)- porphyrins. Journal of Molecular Modeling. 2017; 23 (6):7.
Chicago/Turabian StyleAdrian Calborean; Florin Graur; Vasile Bintintan. 2017. "DFT computational correlations on conformational barriers of Zn2+ and Ni2+ chiral meso-(?,?-unsaturated)- porphyrins." Journal of Molecular Modeling 23, no. 6: 7.
Florin Graur; Emil Mois; Radu Elisei; Luminita Furcea; Mihaela Dragota; Toader Zaharie; Nadim Al Hajjar. Malignant endometriosis of the abdominal wall. Annali italiani di chirurgia 2017, 6, 1 .
AMA StyleFlorin Graur, Emil Mois, Radu Elisei, Luminita Furcea, Mihaela Dragota, Toader Zaharie, Nadim Al Hajjar. Malignant endometriosis of the abdominal wall. Annali italiani di chirurgia. 2017; 6 ():1.
Chicago/Turabian StyleFlorin Graur; Emil Mois; Radu Elisei; Luminita Furcea; Mihaela Dragota; Toader Zaharie; Nadim Al Hajjar. 2017. "Malignant endometriosis of the abdominal wall." Annali italiani di chirurgia 6, no. : 1.