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Small-molecule modulators of TLR8 have drawn much interests as it plays pivotal roles in the innate immune response to single-stranded RNAs (ssRNAs) derived from viruses. However, their clinical uses are limited because they can invoke an uncontrolled, global inflammatory response. The efforts described herein culminate in the fortuitous discovery of a tetrasubstituted imidazole CU-CPD107 which inhibits R848-induced TLR8 signaling. In stark contrast, CU-CPD107 shows unexpected synergistic agonist activities in the presence of ssRNA, while CU-CPD107 alone is unable to influence TLR8 signaling. CU-CPD107’s unique, dichotomous behavior sheds light on a way to approach TLR agonists. CU-CPD107 offers the opportunity to avoid the undesired, global inflammation side effects that have rendered imidazoquinolines clinically irrelevant, providing an insight for the development of antiviral drugs.
Yi Yang; Adam Csakai; Shuangshuang Jiang; Christina Smith; Hiromi Tanji; Jian Huang; Torey Jones; Kentaro Sakaniwa; Lindsey Broadwell; Chengrui Shi; Subada Soti; Umeharu Ohto; Yaohui Fang; Shu Shen; Fei Deng; Toshiyuki Shimizu; Hang Yin. Tetrasubstituted imidazoles as incognito Toll-like receptor 8 a(nta)gonists. Nature Communications 2021, 12, 1 -9.
AMA StyleYi Yang, Adam Csakai, Shuangshuang Jiang, Christina Smith, Hiromi Tanji, Jian Huang, Torey Jones, Kentaro Sakaniwa, Lindsey Broadwell, Chengrui Shi, Subada Soti, Umeharu Ohto, Yaohui Fang, Shu Shen, Fei Deng, Toshiyuki Shimizu, Hang Yin. Tetrasubstituted imidazoles as incognito Toll-like receptor 8 a(nta)gonists. Nature Communications. 2021; 12 (1):1-9.
Chicago/Turabian StyleYi Yang; Adam Csakai; Shuangshuang Jiang; Christina Smith; Hiromi Tanji; Jian Huang; Torey Jones; Kentaro Sakaniwa; Lindsey Broadwell; Chengrui Shi; Subada Soti; Umeharu Ohto; Yaohui Fang; Shu Shen; Fei Deng; Toshiyuki Shimizu; Hang Yin. 2021. "Tetrasubstituted imidazoles as incognito Toll-like receptor 8 a(nta)gonists." Nature Communications 12, no. 1: 1-9.
Crimean-Congo hemorrhagic fever virus (CCHFV) is a widespread, tick-borne pathogen that causes Crimean-Congo hemorrhagic fever (CCHF) with high morbidity and mortality. CCHFV is transmitted to humans through tick bites or direct contact with patients or infected animals with viremia. Currently, climate change and globalization have increased the transmission risk of this biosafety level (BSL)-4 virus. The treatment options of CCHFV infection remain limited and there is no FDA-approved vaccine or specific antivirals, which urges the identification of potential therapeutic targets and the design of CCHF therapies with greater effort. In this article, we discuss the current progress and some future directions in the development of antiviral strategies against CCHFV.
Shiyu Dai; Fei Deng; Hualin Wang; Yunjia Ning. Crimean-Congo Hemorrhagic Fever Virus: Current Advances and Future Prospects of Antiviral Strategies. Viruses 2021, 13, 1195 .
AMA StyleShiyu Dai, Fei Deng, Hualin Wang, Yunjia Ning. Crimean-Congo Hemorrhagic Fever Virus: Current Advances and Future Prospects of Antiviral Strategies. Viruses. 2021; 13 (7):1195.
Chicago/Turabian StyleShiyu Dai; Fei Deng; Hualin Wang; Yunjia Ning. 2021. "Crimean-Congo Hemorrhagic Fever Virus: Current Advances and Future Prospects of Antiviral Strategies." Viruses 13, no. 7: 1195.
COVID-19 pandemic caused by SARS-CoV-2 constitutes a global public health crisis with enormous economic consequences. Monoclonal antibodies against SARS-CoV-2 can provide an important treatment option to fight COVID-19, especially for the most vulnerable populations. In this work, potent antibodies binding to SARS-CoV-2 Spike protein were identified from COVID-19 convalescent patients. Among them, P4A1 interacts directly with and covers majority of the Receptor Binding Motif of the Spike Receptor-Binding Domain, shown by high-resolution complex structure analysis. We further demonstrate the binding and neutralizing activities of P4A1 against wild type and mutant Spike proteins or pseudoviruses. P4A1 was subsequently engineered to reduce the potential risk for Antibody-Dependent Enhancement of infection and to extend its half-life. The engineered antibody exhibits an optimized pharmacokinetic and safety profile, and it results in complete viral clearance in a rhesus monkey model of COVID-19 following a single injection. These data suggest its potential against SARS-CoV-2 related diseases.
Yu Guo; Lisu Huang; Guangshun Zhang; Yanfeng Yao; He Zhou; Shu Shen; Bingqing Shen; Bo Li; Xin Li; Qian Zhang; Mingjie Chen; Da Chen; Jia Wu; Dan Fu; Xinxin Zeng; Mingfang Feng; ChunJiang Pi; Yuan Wang; Xingdong Zhou; Minmin Lu; Yarong Li; Yaohui Fang; Yun-Yueh Lu; Xue Hu; Shanshan Wang; Wanju Zhang; Ge Gao; Francisco Adrian; Qisheng Wang; Feng Yu; Yun Peng; Alexander G. Gabibov; Juan Min; Yuhui Wang; Heyu Huang; Alexey Stepanov; Wei Zhang; Yan Cai; Junwei Liu; Zhiming Yuan; Chen Zhang; Zhiyong Lou; Fei Deng; Hongkai Zhang; Chao Shan; Liang Schweizer; Kun Sun; Zihe Rao. A SARS-CoV-2 neutralizing antibody with extensive Spike binding coverage and modified for optimal therapeutic outcomes. Nature Communications 2021, 12, 1 -11.
AMA StyleYu Guo, Lisu Huang, Guangshun Zhang, Yanfeng Yao, He Zhou, Shu Shen, Bingqing Shen, Bo Li, Xin Li, Qian Zhang, Mingjie Chen, Da Chen, Jia Wu, Dan Fu, Xinxin Zeng, Mingfang Feng, ChunJiang Pi, Yuan Wang, Xingdong Zhou, Minmin Lu, Yarong Li, Yaohui Fang, Yun-Yueh Lu, Xue Hu, Shanshan Wang, Wanju Zhang, Ge Gao, Francisco Adrian, Qisheng Wang, Feng Yu, Yun Peng, Alexander G. Gabibov, Juan Min, Yuhui Wang, Heyu Huang, Alexey Stepanov, Wei Zhang, Yan Cai, Junwei Liu, Zhiming Yuan, Chen Zhang, Zhiyong Lou, Fei Deng, Hongkai Zhang, Chao Shan, Liang Schweizer, Kun Sun, Zihe Rao. A SARS-CoV-2 neutralizing antibody with extensive Spike binding coverage and modified for optimal therapeutic outcomes. Nature Communications. 2021; 12 (1):1-11.
Chicago/Turabian StyleYu Guo; Lisu Huang; Guangshun Zhang; Yanfeng Yao; He Zhou; Shu Shen; Bingqing Shen; Bo Li; Xin Li; Qian Zhang; Mingjie Chen; Da Chen; Jia Wu; Dan Fu; Xinxin Zeng; Mingfang Feng; ChunJiang Pi; Yuan Wang; Xingdong Zhou; Minmin Lu; Yarong Li; Yaohui Fang; Yun-Yueh Lu; Xue Hu; Shanshan Wang; Wanju Zhang; Ge Gao; Francisco Adrian; Qisheng Wang; Feng Yu; Yun Peng; Alexander G. Gabibov; Juan Min; Yuhui Wang; Heyu Huang; Alexey Stepanov; Wei Zhang; Yan Cai; Junwei Liu; Zhiming Yuan; Chen Zhang; Zhiyong Lou; Fei Deng; Hongkai Zhang; Chao Shan; Liang Schweizer; Kun Sun; Zihe Rao. 2021. "A SARS-CoV-2 neutralizing antibody with extensive Spike binding coverage and modified for optimal therapeutic outcomes." Nature Communications 12, no. 1: 1-11.
Evidence suggests that platelets may directly interact with SARS-CoV-2, raising the concern whether ACE2 receptor plays a role in this interaction. The current study showed that SARS-CoV-2 interacts with both platelets and megakaryocytes despite the limited efficiency. Abundance of the conventional receptor ACE2 and alternative receptors or co-factors for SARS-CoV-2 entry was characterized in platelets from COVID-19 patients and healthy persons as well as human megakaryocytes based on laboratory tests or previously reported RNA-seq data. The results suggest that SARS-CoV-2 interacts with platelets and megakaryocytes via ACE2-independent mechanism and may regulate alternative receptor expression associated with COVID-19 coagulation dysfunction.
Shu Shen; Jingyuan Zhang; Yaohui Fang; Sihong Lu; Jun Wu; Xin Zheng; Fei Deng. SARS-CoV-2 interacts with platelets and megakaryocytes via ACE2-independent mechanism. Journal of Hematology & Oncology 2021, 14, 1 -5.
AMA StyleShu Shen, Jingyuan Zhang, Yaohui Fang, Sihong Lu, Jun Wu, Xin Zheng, Fei Deng. SARS-CoV-2 interacts with platelets and megakaryocytes via ACE2-independent mechanism. Journal of Hematology & Oncology. 2021; 14 (1):1-5.
Chicago/Turabian StyleShu Shen; Jingyuan Zhang; Yaohui Fang; Sihong Lu; Jun Wu; Xin Zheng; Fei Deng. 2021. "SARS-CoV-2 interacts with platelets and megakaryocytes via ACE2-independent mechanism." Journal of Hematology & Oncology 14, no. 1: 1-5.
Wuhan was the very first city hit by SARS-CoV-2. Accordingly, the patients who experienced the longest phase of convalescence following COVID-19 reside here.
Jia Liu; Xuecheng Yang; Hua Wang; Ziwei Li; Hui Deng; Jing Liu; Shue Xiong; Junyi He; Xuemei Feng; Chunxia Guo; Weixian Wang; Gennadiy Zelinskyy; Mirko Trilling; Kathrin Sutter; Tina Senff; Christopher Menne; Joerg Timm; Yanfang Zhang; Fei Deng; Yinping Lu; Jun Wu; Mengji Lu; Dongliang Yang; Ulf Dittmer; Baoju Wang; Xin Zheng. Analysis of the Long-Term Impact on Cellular Immunity in COVID-19-Recovered Individuals Reveals a Profound NKT Cell Impairment. mBio 2021, 12, 1 .
AMA StyleJia Liu, Xuecheng Yang, Hua Wang, Ziwei Li, Hui Deng, Jing Liu, Shue Xiong, Junyi He, Xuemei Feng, Chunxia Guo, Weixian Wang, Gennadiy Zelinskyy, Mirko Trilling, Kathrin Sutter, Tina Senff, Christopher Menne, Joerg Timm, Yanfang Zhang, Fei Deng, Yinping Lu, Jun Wu, Mengji Lu, Dongliang Yang, Ulf Dittmer, Baoju Wang, Xin Zheng. Analysis of the Long-Term Impact on Cellular Immunity in COVID-19-Recovered Individuals Reveals a Profound NKT Cell Impairment. mBio. 2021; 12 (2):1.
Chicago/Turabian StyleJia Liu; Xuecheng Yang; Hua Wang; Ziwei Li; Hui Deng; Jing Liu; Shue Xiong; Junyi He; Xuemei Feng; Chunxia Guo; Weixian Wang; Gennadiy Zelinskyy; Mirko Trilling; Kathrin Sutter; Tina Senff; Christopher Menne; Joerg Timm; Yanfang Zhang; Fei Deng; Yinping Lu; Jun Wu; Mengji Lu; Dongliang Yang; Ulf Dittmer; Baoju Wang; Xin Zheng. 2021. "Analysis of the Long-Term Impact on Cellular Immunity in COVID-19-Recovered Individuals Reveals a Profound NKT Cell Impairment." mBio 12, no. 2: 1.
A Correction to this paper has been published: https://doi.org/10.1038/s41928-021-00582-0.
Panpan Wang; Lu Liu; Aijun Liu; Liming Yan; Yong He; Shu Shen; Mingxu Hu; Yu Guo; Haiguang Liu; Chuang Liu; Yinying Lu; Peiyi Wang; Fei Deng; Zihe Rao; Zhiyong Lou. Author Correction: Structure of severe fever with thrombocytopenia syndrome virus L protein elucidates the mechanisms of viral transcription initiation. Nature Microbiology 2021, 6, 697 -698.
AMA StylePanpan Wang, Lu Liu, Aijun Liu, Liming Yan, Yong He, Shu Shen, Mingxu Hu, Yu Guo, Haiguang Liu, Chuang Liu, Yinying Lu, Peiyi Wang, Fei Deng, Zihe Rao, Zhiyong Lou. Author Correction: Structure of severe fever with thrombocytopenia syndrome virus L protein elucidates the mechanisms of viral transcription initiation. Nature Microbiology. 2021; 6 (5):697-698.
Chicago/Turabian StylePanpan Wang; Lu Liu; Aijun Liu; Liming Yan; Yong He; Shu Shen; Mingxu Hu; Yu Guo; Haiguang Liu; Chuang Liu; Yinying Lu; Peiyi Wang; Fei Deng; Zihe Rao; Zhiyong Lou. 2021. "Author Correction: Structure of severe fever with thrombocytopenia syndrome virus L protein elucidates the mechanisms of viral transcription initiation." Nature Microbiology 6, no. 5: 697-698.
The wide-spreading SARS-CoV-2 virus has put the world into boiling water for more than a year, however pharmacological therapies to act effectively against coronavirus disease 2019 (COVID-19) remain elusive. Chloroquine (CQ), an antimalarial drug, was found to exhibit promising antiviral activity in vitro and in vivo at a high dosage, thus CQ was approved by the FDA for the emergency use authorization (EUA) in the fight against COVID-19 in the US, but later was revoked the EUA status due to the severe clinical toxicity. Herein, we show that supramolecular formulation of CQ by a macrocyclic host, curcurbit[7]uril (CB[7]), reduced its non-specific toxicity and improved its antiviral activity against coronavirus, working in synergy with CB[7]. CB[7] was found to form 1:1 host-guest complexes with CQ, with a binding constant of ∼104 L/mol. The CQ-CB[7] formulation decreased the cytotoxicity of CQ against Vero E6 and L-02 cell lines. In particular, the cytotoxicity of CQ (60 μmol/L) against both Vero E6 cell line and L-02 cell lines was completely inhibited in the presence of 300 μmol/L and 600 μmol/L CB[7], respectively. Furthermore, the CB[7] alone showed astonishing antiviral activity in SARS-CoV-2 infected Vero E6 cells and mouse hepatitis virus strain A59 (MHV-A59) infected N2A cells, and synergistically improved the antiviral activity of CQ-CB[7], suggesting that CB[7]-based CQ formulation has a great potential as a safe and effective antiviral agent against SARS-CoV-2 and other coronavirus.
Cheryl H.T. Kwong; Jingfang Mu; Shengke Li; Yaohui Fang; Qianyun Liu; Xiangjun Zhang; Hiotong Kam; Simon M.Y. Lee; Yu Chen; Fei Deng; Xi Zhou; Ruibing Wang. Reviving chloroquine for anti-SARS-CoV-2 treatment with cucurbit[7]uril-based supramolecular formulation. Chinese Chemical Letters 2021, 1 .
AMA StyleCheryl H.T. Kwong, Jingfang Mu, Shengke Li, Yaohui Fang, Qianyun Liu, Xiangjun Zhang, Hiotong Kam, Simon M.Y. Lee, Yu Chen, Fei Deng, Xi Zhou, Ruibing Wang. Reviving chloroquine for anti-SARS-CoV-2 treatment with cucurbit[7]uril-based supramolecular formulation. Chinese Chemical Letters. 2021; ():1.
Chicago/Turabian StyleCheryl H.T. Kwong; Jingfang Mu; Shengke Li; Yaohui Fang; Qianyun Liu; Xiangjun Zhang; Hiotong Kam; Simon M.Y. Lee; Yu Chen; Fei Deng; Xi Zhou; Ruibing Wang. 2021. "Reviving chloroquine for anti-SARS-CoV-2 treatment with cucurbit[7]uril-based supramolecular formulation." Chinese Chemical Letters , no. : 1.
Long-term antibody responses and neutralizing activities in response to SARS-CoV-2 infection are not yet clear. Here we quantify immunoglobulin M (IgM) and G (IgG) antibodies recognizing the SARS-CoV-2 receptor-binding domain (RBD) of the spike (S) or the nucleocapsid (N) protein, and neutralizing antibodies during a period of 6 months from COVID-19 disease onset in 349 symptomatic COVID-19 patients who were among the first be infected world-wide. The positivity rate and magnitude of IgM-S and IgG-N responses increase rapidly. High levels of IgM-S/N and IgG-S/N at 2-3 weeks after disease onset are associated with virus control and IgG-S titers correlate closely with the capacity to neutralize SARS-CoV-2. Although specific IgM-S/N become undetectable 12 weeks after disease onset in most patients, IgG-S/N titers have an intermediate contraction phase, but stabilize at relatively high levels over the 6 month observation period. At late time points, the positivity rates for binding and neutralizing SARS-CoV-2-specific antibodies are still >70%. These data indicate sustained humoral immunity in recovered patients who had symptomatic COVID-19, suggesting prolonged immunity.
Jun Wu; Boyun Liang; Cunrong Chen; Hua Wang; Yaohui Fang; Shu Shen; Xiaoli Yang; Baoju Wang; Liangkai Chen; Qi Chen; Yang Wu; Jia Liu; Xuecheng Yang; Wei Li; Bin Zhu; Wenqing Zhou; Sumeng Li; Sihong Lu; Di Liu; Huadong Li; Adalbert Krawczyk; Mengji Lu; Dongliang Yang; Fei Deng; Ulf Dittmer; Mirko Trilling; Xin Zheng. SARS-CoV-2 infection induces sustained humoral immune responses in convalescent patients following symptomatic COVID-19. Nature Communications 2021, 12, 1 -9.
AMA StyleJun Wu, Boyun Liang, Cunrong Chen, Hua Wang, Yaohui Fang, Shu Shen, Xiaoli Yang, Baoju Wang, Liangkai Chen, Qi Chen, Yang Wu, Jia Liu, Xuecheng Yang, Wei Li, Bin Zhu, Wenqing Zhou, Sumeng Li, Sihong Lu, Di Liu, Huadong Li, Adalbert Krawczyk, Mengji Lu, Dongliang Yang, Fei Deng, Ulf Dittmer, Mirko Trilling, Xin Zheng. SARS-CoV-2 infection induces sustained humoral immune responses in convalescent patients following symptomatic COVID-19. Nature Communications. 2021; 12 (1):1-9.
Chicago/Turabian StyleJun Wu; Boyun Liang; Cunrong Chen; Hua Wang; Yaohui Fang; Shu Shen; Xiaoli Yang; Baoju Wang; Liangkai Chen; Qi Chen; Yang Wu; Jia Liu; Xuecheng Yang; Wei Li; Bin Zhu; Wenqing Zhou; Sumeng Li; Sihong Lu; Di Liu; Huadong Li; Adalbert Krawczyk; Mengji Lu; Dongliang Yang; Fei Deng; Ulf Dittmer; Mirko Trilling; Xin Zheng. 2021. "SARS-CoV-2 infection induces sustained humoral immune responses in convalescent patients following symptomatic COVID-19." Nature Communications 12, no. 1: 1-9.
Age is a risk factor for coronavirus disease 2019 (COVID-19)-associated morbidity and mortality in humans; hence, in this study, we compared the course of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection in young and aged BALB/c mice. We found that SARS-CoV-2 isolates replicated in the respiratory tracts of 12-month-old (aged) mice and caused pathological features of pneumonia upon intranasal infection. In contrast, rapid viral clearance was observed 5 days following infection in 2-month-old (young) mice with no evidence of pathological changes in the lungs. Infection with SARS-CoV-2 elicited significantly upregulated production of cytokines, especially interleukin 6 and interferon gamma, in aged mice, whereas this response was much weaker in young mice. Subsequent challenge of infected aged BALB/c mice with SARS-CoV-2 resulted in neutralized antibody responses, a significantly reduced viral burden in the lungs, and inflammation mitigation. Deep sequencing showed a panel of mutations potentially associated with the enhanced infection in aged BALB/c mice, such as the Q498H mutation, which is located at the receptor-binding domain (RBD) of the spike (S) protein. We further found that the isolates cannot only multiply in the respiratory tract of mice, but also cause disease in aged mice. Overall, viral replication and rapid adaption in aged BALB/c mice were associated with pneumonia, confirming that the age-related susceptibility to SARS-CoV-2 in mice resembled that in humans. IMPORTANCE Aged BALB/c mice are in use as a model of disease caused by SARS-CoV-2. Our research demonstrated SARS-CoV-2 can rapidly adapt in aged BALB/c mice through causing mutations at the RBD of the S protein. Moreover, SARS-CoV-2-infected aged BALB/c mice indicated that alveolar damage, interstitial pneumonia, and inflammatory immune responses were similar to the clinical manifestations of human infections. Therefore, our aged BALB/c challenge model will be useful for further understanding the pathogenesis of SARS-CoV-2 and for testing vaccines and antiviral agents.
Yufei Zhang; Kun Huang; Ting Wang; Fei Deng; Wenxiao Gong; Xianfeng Hui; Ya Zhao; Xinlin He; Chengfei Li; Qiang Zhang; Xi Chen; Changjie Lv; Xian Lin; Ying Yang; Xiaomei Sun; Zhengli Shi; Huanchun Chen; Zhong Zou; Meilin Jin. SARS-CoV-2 Rapidly Adapts in Aged BALB/c Mice and Induces Typical Pneumonia. Journal of Virology 2021, 95, 1 .
AMA StyleYufei Zhang, Kun Huang, Ting Wang, Fei Deng, Wenxiao Gong, Xianfeng Hui, Ya Zhao, Xinlin He, Chengfei Li, Qiang Zhang, Xi Chen, Changjie Lv, Xian Lin, Ying Yang, Xiaomei Sun, Zhengli Shi, Huanchun Chen, Zhong Zou, Meilin Jin. SARS-CoV-2 Rapidly Adapts in Aged BALB/c Mice and Induces Typical Pneumonia. Journal of Virology. 2021; 95 (11):1.
Chicago/Turabian StyleYufei Zhang; Kun Huang; Ting Wang; Fei Deng; Wenxiao Gong; Xianfeng Hui; Ya Zhao; Xinlin He; Chengfei Li; Qiang Zhang; Xi Chen; Changjie Lv; Xian Lin; Ying Yang; Xiaomei Sun; Zhengli Shi; Huanchun Chen; Zhong Zou; Meilin Jin. 2021. "SARS-CoV-2 Rapidly Adapts in Aged BALB/c Mice and Induces Typical Pneumonia." Journal of Virology 95, no. 11: 1.
As of August 23, 2020, the 2019 novel coronavirus disease (COVID-19) has infected more than 23,518,340 people and caused more than 810,492 deaths worldwide including 4,717 deaths in China. We present a case of a 53-year-old woman who was admitted to the hospital because of dry coughs and high fever on January 26, 2020, in Wuhan, China. She was not tested for SARS-CoV-2 RNA until on hospital day 11 (illness day 21) because of a significant shortage of test kits at the local hospital. Then, her test was positive for COVID-19 on hospital day 20. Despite intensive medical treatments, she developed respiratory failure with secondary bacterial infection and expired on hospital day 23 (3 days after she was tested positive for SARS-CoV-2 RNA). A systemic autopsy examination, including immunohistochemistry and ultrastructural studies, demonstrates that SARS-CoV-2 can infect multiple organs with profound adverse effect on the immune system, and the lung pathology is characterized by diffuse alveolar damage. Extrapulmonary SARS-CoV-2 RNA was detected in several organs postmortem. The detailed pathological features are described. In addition, this report highlights the value of forensic autopsy in studying SARS-CoV-2 infection and the importance of clinicopathological correlation in better understanding the pathogenesis of COVID-19.
Liang Ren; Qian Liu; Rongshuai Wang; Rong Chen; Qilin Ao; Xi Wang; Jie Zhang; Fei Deng; Yan Feng; Guoping Wang; Yiwu Zhou; Ling Li; Liang Liu. Clinicopathologic Features of COVID-19: A Case Report and Value of Forensic Autopsy in Studying SARS-CoV-2 Infection. American Journal of Forensic Medicine & Pathology 2021, 42, 164 -169.
AMA StyleLiang Ren, Qian Liu, Rongshuai Wang, Rong Chen, Qilin Ao, Xi Wang, Jie Zhang, Fei Deng, Yan Feng, Guoping Wang, Yiwu Zhou, Ling Li, Liang Liu. Clinicopathologic Features of COVID-19: A Case Report and Value of Forensic Autopsy in Studying SARS-CoV-2 Infection. American Journal of Forensic Medicine & Pathology. 2021; 42 (2):164-169.
Chicago/Turabian StyleLiang Ren; Qian Liu; Rongshuai Wang; Rong Chen; Qilin Ao; Xi Wang; Jie Zhang; Fei Deng; Yan Feng; Guoping Wang; Yiwu Zhou; Ling Li; Liang Liu. 2021. "Clinicopathologic Features of COVID-19: A Case Report and Value of Forensic Autopsy in Studying SARS-CoV-2 Infection." American Journal of Forensic Medicine & Pathology 42, no. 2: 164-169.
Ticks are well known as vectors of many viruses which usually do great harm to human and animal health. Yunnan Province, widely covered by flourishing vegetation and mainly relying on farming husbandry, is abundant with Rhipicephalus microplus ticks. Therefore, it is of great significance to characterize the viral profile present in R. microplus parasitizing on cattle in Yunnan Province. In this study, a total of 7387 R. microplus ticks were collected from cattle and buffalo in the northwest and southeast areas of Yunnan Province from 2015 to 2017. We investigated the virome of R. microplus using next-generation sequencing (NGS) and the prevalence of important identified viruses among tick groups by RT-PCR. It revealed the presence of diverse virus concerning chu-, rhabdo-, phlebo-, flavi- and parvo- viruses in Yunnan. These viruses consist of single-stranded, circular and segmented sense RNAs, showing a greatly diversity in genomic organization. Furthermore, continuous epidemiological survey among ticks reveals broad prevalence of three viruses (Yunnan mivirus 1, Wuhan tick vrius 1 and YN tick-associated phlebovirus 1) and two possible prevalent viruses including a flavivirus-like segmented virus (Jingmen tick virus) and a bovine hokovirus 2 in Yunnan. Serological investigation among cattle indicates that these identified viruses may be infectious to cattle and can elicit corresponding antibody. Our findings on R. microplus-associated viral community will contribute to the prevention of viral disease and tracking the viral evolution. Further analysis is needed to better elucidate the pathogenicity and natural circulation of these viruses.
Junming Shi; Shu Shen; Hui Wu; Yunzhi Zhang; Fei Deng. Metagenomic Profiling of Viruses Associated with Rhipicephalus microplus Ticks in Yunnan Province, China. Virologica Sinica 2021, 36, 623 -635.
AMA StyleJunming Shi, Shu Shen, Hui Wu, Yunzhi Zhang, Fei Deng. Metagenomic Profiling of Viruses Associated with Rhipicephalus microplus Ticks in Yunnan Province, China. Virologica Sinica. 2021; 36 (4):623-635.
Chicago/Turabian StyleJunming Shi; Shu Shen; Hui Wu; Yunzhi Zhang; Fei Deng. 2021. "Metagenomic Profiling of Viruses Associated with Rhipicephalus microplus Ticks in Yunnan Province, China." Virologica Sinica 36, no. 4: 623-635.
Bunyavirus ribonucleoprotein (RNP) that is assembled by polymerized nucleoproteins (N) coating a viral RNA and associating with a viral polymerase can be both the RNA synthesis machinery and the structural core of virions. Bunyaviral N and RNP thus could be assailable targets for host antiviral defense; however, it remains unclear which and how host factors target N/RNP to restrict bunyaviral infection. By mass spectrometry and protein-interaction analyses, we here show that host protein MOV10 targets the N proteins encoded by a group of emerging high-pathogenic representatives of bunyaviruses including severe fever with thrombocytopenia syndrome virus (SFTSV), one of the most dangerous pathogens listed by World Health Organization, in RNA-independent manner. MOV10 that was further shown to be induced specifically by SFTSV and related bunyaviruses in turn inhibits the bunyaviral replication in infected cells in series of loss/gain-of-function assays. Moreover, animal infection experiments with MOV10 knockdown corroborated the role of MOV10 in restricting SFTSV infection and pathogenicity in vivo. Minigenome assays and additional functional and mechanistic investigations demonstrate that the anti-bunyavirus activity of MOV10 is likely achieved by direct impact on viral RNP machinery but independent of its helicase activity and the cellular interferon pathway. Indeed, by its N-terminus, MOV10 binds to a protruding N-arm domain of N consisting of only 34 amino acids but proving important for N function and blocks N polymerization, N-RNA binding, and N-polymerase interaction, disabling RNP assembly. This study not only advances the understanding of bunyaviral replication and host restriction mechanisms but also presents novel paradigms for both direct antiviral action of MOV10 and host targeting of viral RNP machinery.
Qiong Mo; Zhao Xu; Fei Deng; Hualin Wang; Yun-Jia Ning. Host restriction of emerging high-pathogenic bunyaviruses via MOV10 by targeting viral nucleoprotein and blocking ribonucleoprotein assembly. PLOS Pathogens 2020, 16, e1009129 .
AMA StyleQiong Mo, Zhao Xu, Fei Deng, Hualin Wang, Yun-Jia Ning. Host restriction of emerging high-pathogenic bunyaviruses via MOV10 by targeting viral nucleoprotein and blocking ribonucleoprotein assembly. PLOS Pathogens. 2020; 16 (12):e1009129.
Chicago/Turabian StyleQiong Mo; Zhao Xu; Fei Deng; Hualin Wang; Yun-Jia Ning. 2020. "Host restriction of emerging high-pathogenic bunyaviruses via MOV10 by targeting viral nucleoprotein and blocking ribonucleoprotein assembly." PLOS Pathogens 16, no. 12: e1009129.
The Coronavirus Disease of 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) threatens global public health and economy. Therapeutic options such as monoclonal antibodies (mAbs) against SARS-CoV-2 are in urgent need. We have identified potent monoclonal antibodies binding to SARS-CoV-2 Spike protein from COVID-19 convalescent patients and one of these antibodies, P4A1, interacts directly and covers the majority of the Receptor Binding Motif (RBM) of Spike receptor-binding domain (RBD), shown by high-resolution complex structure analysis. We further demonstrated P4A1 binding and neutralizing activities against wild type and mutant spike proteins. P4A1 was subsequently engineered to reduce the potential risk for antibody-dependent enhancement (ADE) of infection and to extend its half-life. The engineered mAb exhibits optimized pharmacokinetic and safety profile, and results in complete viral clearance in a rhesus monkey model of COVID-19 following a single injection.
Yu Guo; Lisu Huang; Guangshun Zhang; Yanfeng Yao; He Zhou; Shu Shen; Bingqing Shen; Bo Li; Xin Li; Mingjie Chen; Da Chen; Jia Wu; Dan Fu; Xinxin Zeng; Mingfang Feng; ChunJiang Pi; Yuan Wang; Xingdong Zhou; Minmin Lu; Yaohui Fang; Yun-Yueh Lu; Xue Hu; Shanshan Wang; Wanju Zhang; Qian Zhang; Ge Gao; Francisco Adrian; Qisheng Wang; Feng Yu; Yun Peng; Alexander Gabibov; Juan Min; Yuhui Wang; Heyu Huang; Alexey Stepanov; Wei Zhang; Yan Cai; Junwei Liu; Zhi-Ming Yuan; Chen Zhang; Zhiyong Lou; Fei Deng; Hongkai Zhang; Chao Shan; Liang Schweizer; Kun Sun; Zihe Rao. A SARS-CoV-2 neutralizing antibody with exceptional spike binding coverage and optimized therapeutic potentials. 2020, 1 .
AMA StyleYu Guo, Lisu Huang, Guangshun Zhang, Yanfeng Yao, He Zhou, Shu Shen, Bingqing Shen, Bo Li, Xin Li, Mingjie Chen, Da Chen, Jia Wu, Dan Fu, Xinxin Zeng, Mingfang Feng, ChunJiang Pi, Yuan Wang, Xingdong Zhou, Minmin Lu, Yaohui Fang, Yun-Yueh Lu, Xue Hu, Shanshan Wang, Wanju Zhang, Qian Zhang, Ge Gao, Francisco Adrian, Qisheng Wang, Feng Yu, Yun Peng, Alexander Gabibov, Juan Min, Yuhui Wang, Heyu Huang, Alexey Stepanov, Wei Zhang, Yan Cai, Junwei Liu, Zhi-Ming Yuan, Chen Zhang, Zhiyong Lou, Fei Deng, Hongkai Zhang, Chao Shan, Liang Schweizer, Kun Sun, Zihe Rao. A SARS-CoV-2 neutralizing antibody with exceptional spike binding coverage and optimized therapeutic potentials. . 2020; ():1.
Chicago/Turabian StyleYu Guo; Lisu Huang; Guangshun Zhang; Yanfeng Yao; He Zhou; Shu Shen; Bingqing Shen; Bo Li; Xin Li; Mingjie Chen; Da Chen; Jia Wu; Dan Fu; Xinxin Zeng; Mingfang Feng; ChunJiang Pi; Yuan Wang; Xingdong Zhou; Minmin Lu; Yaohui Fang; Yun-Yueh Lu; Xue Hu; Shanshan Wang; Wanju Zhang; Qian Zhang; Ge Gao; Francisco Adrian; Qisheng Wang; Feng Yu; Yun Peng; Alexander Gabibov; Juan Min; Yuhui Wang; Heyu Huang; Alexey Stepanov; Wei Zhang; Yan Cai; Junwei Liu; Zhi-Ming Yuan; Chen Zhang; Zhiyong Lou; Fei Deng; Hongkai Zhang; Chao Shan; Liang Schweizer; Kun Sun; Zihe Rao. 2020. "A SARS-CoV-2 neutralizing antibody with exceptional spike binding coverage and optimized therapeutic potentials." , no. : 1.
Xiaoli Wu; Mingyue Li; Yanfang Zhang; Boyun Liang; Junming Shi; Yaohui Fang; Zhengyuan Su; Mengmeng Li; Wenjing Zhang; Ling Xu; Jun Wang; Qiaoli Wu; Shuang Tang; Hualin Wang; Tao Zhang; Cheng Peng; Xin Zheng; Fei Deng; Shu Shen. Novel SFTSV Phylogeny Reveals New Reassortment Events and Migration Routes. Virologica Sinica 2020, 36, 300 -310.
AMA StyleXiaoli Wu, Mingyue Li, Yanfang Zhang, Boyun Liang, Junming Shi, Yaohui Fang, Zhengyuan Su, Mengmeng Li, Wenjing Zhang, Ling Xu, Jun Wang, Qiaoli Wu, Shuang Tang, Hualin Wang, Tao Zhang, Cheng Peng, Xin Zheng, Fei Deng, Shu Shen. Novel SFTSV Phylogeny Reveals New Reassortment Events and Migration Routes. Virologica Sinica. 2020; 36 (2):300-310.
Chicago/Turabian StyleXiaoli Wu; Mingyue Li; Yanfang Zhang; Boyun Liang; Junming Shi; Yaohui Fang; Zhengyuan Su; Mengmeng Li; Wenjing Zhang; Ling Xu; Jun Wang; Qiaoli Wu; Shuang Tang; Hualin Wang; Tao Zhang; Cheng Peng; Xin Zheng; Fei Deng; Shu Shen. 2020. "Novel SFTSV Phylogeny Reveals New Reassortment Events and Migration Routes." Virologica Sinica 36, no. 2: 300-310.
The coronavirus disease 2019 (COVID-19) pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) affects millions of people and killed hundred-thousands of individuals. While acute and intermediate interactions between SARS-CoV-2 and the immune system have been studied extensively, long-term impacts on the cellular immune system remained to be analyzed. Here, we comprehensively characterized immunological changes in peripheral blood mononuclear cells in 49 COVID-19 convalescent individuals (CI) in comparison to 27 matched SARS-CoV-2 unexposed individuals (UI). Despite recovery from the disease for more than 2 months, CI showed significant decreases in frequencies of invariant NKT and NKT-like cells compared to UI. Concomitant with the decrease in NKT-like cells, an increase in the percentage of Annexin V and 7-AAD double positive NKT-like cells was detected, suggesting that the reduction in NKT-like cells results from cell death months after recovery. Significant increases in regulatory T cell frequencies, TIM-3 expression on CD4 and CD8 T cells, as well as PD-L1 expression on B cells were also observed in CI, while the cytotoxic potential of T cells and NKT-like cells, defined by GzmB expression, was significantly diminished. However, both CD4 and CD8 T cells of CI showed increased Ki67 expression and were fully capable to proliferate and produce effector cytokines upon TCR stimulation. Collectively, we provide the first comprehensive characterization of immune signatures in patients recovering from SARS-CoV-2 infection, suggesting that the cellular immune system of COVID-19 patients is still under a sustained influence even months after the recovery from disease.
Jia Liu; Xuecheng Yang; Hua Wang; Ziwei Li; Hui Deng; Jing Liu; Shue Xiong; Junyi He; Chunxia Guo; Weixian Wang; Gennadiy Zelinskyy; Mirko Trilling; Ulf Dittmer; Mengji Lu; Kathrin Sutter; Tina Senff; Christopher Menne; Joerg Timm; Yanfang Zhang; Fei Deng; Xuemei Feng; Yinping Lu; Jun Wu; Dongliang Yang; Baoju Wang; Xin Zheng. The analysis of the long-term impact of SARS-CoV-2 on the cellular immune system in individuals recovering from COVID-19 reveals a profound NKT cell impairment. 2020, 1 .
AMA StyleJia Liu, Xuecheng Yang, Hua Wang, Ziwei Li, Hui Deng, Jing Liu, Shue Xiong, Junyi He, Chunxia Guo, Weixian Wang, Gennadiy Zelinskyy, Mirko Trilling, Ulf Dittmer, Mengji Lu, Kathrin Sutter, Tina Senff, Christopher Menne, Joerg Timm, Yanfang Zhang, Fei Deng, Xuemei Feng, Yinping Lu, Jun Wu, Dongliang Yang, Baoju Wang, Xin Zheng. The analysis of the long-term impact of SARS-CoV-2 on the cellular immune system in individuals recovering from COVID-19 reveals a profound NKT cell impairment. . 2020; ():1.
Chicago/Turabian StyleJia Liu; Xuecheng Yang; Hua Wang; Ziwei Li; Hui Deng; Jing Liu; Shue Xiong; Junyi He; Chunxia Guo; Weixian Wang; Gennadiy Zelinskyy; Mirko Trilling; Ulf Dittmer; Mengji Lu; Kathrin Sutter; Tina Senff; Christopher Menne; Joerg Timm; Yanfang Zhang; Fei Deng; Xuemei Feng; Yinping Lu; Jun Wu; Dongliang Yang; Baoju Wang; Xin Zheng. 2020. "The analysis of the long-term impact of SARS-CoV-2 on the cellular immune system in individuals recovering from COVID-19 reveals a profound NKT cell impairment." , no. : 1.
Xin Zheng; Hua Wang; Zhengyuan Su; Wei Li; Dongliang Yang; Fei Deng; Jianjun Chen. Co-infection of SARS-CoV-2 and Influenza virus in Early Stage of the COVID-19 Epidemic in Wuhan, China. Journal of Infection 2020, 81, e128 -e129.
AMA StyleXin Zheng, Hua Wang, Zhengyuan Su, Wei Li, Dongliang Yang, Fei Deng, Jianjun Chen. Co-infection of SARS-CoV-2 and Influenza virus in Early Stage of the COVID-19 Epidemic in Wuhan, China. Journal of Infection. 2020; 81 (2):e128-e129.
Chicago/Turabian StyleXin Zheng; Hua Wang; Zhengyuan Su; Wei Li; Dongliang Yang; Fei Deng; Jianjun Chen. 2020. "Co-infection of SARS-CoV-2 and Influenza virus in Early Stage of the COVID-19 Epidemic in Wuhan, China." Journal of Infection 81, no. 2: e128-e129.
Similar to some large DNA viruses that encode their own disulfide bond pathway, baculovirus encodes a viral sulfhydryl oxidase, P33. Enzyme activity of P33 is related to infectious BV production, occlusion-derived virus (ODV) envelopment, occlusion body morphogenesis, and oral infectivity, suggesting that P33 is involved in disulfide bond formation of multiple proteins. A complete disulfide bond formation pathway normally contains a sulfhydryl oxidase, a disulfide-donating enzyme, and one or more substrates. In baculovirus, apart from P33, other components of the putative pathway remain unknown. In this study, we identified PIF5 as the first substrate of P33, which is fundamental for revealing the complete disulfide bond formation pathway in baculovirus. PIF5 is essential for oral infection and is absent from the PIF complex. Our study demonstrated that native disulfide bonds in PIF5 are required for oral infection, which will help us to reveal its mode of action.
Huanyu Zhang; Wenhua Kuang; Cheng Chen; Yu Shang; Xiaoyan Ma; Fei Deng; Hualin Wang; Manli Wang; Zhihong Hu. Per Os Infectivity Factor 5 Identified as a Substrate of P33 in the Baculoviral Disulfide Bond Formation Pathway. Journal of Virology 2020, 94, 1 .
AMA StyleHuanyu Zhang, Wenhua Kuang, Cheng Chen, Yu Shang, Xiaoyan Ma, Fei Deng, Hualin Wang, Manli Wang, Zhihong Hu. Per Os Infectivity Factor 5 Identified as a Substrate of P33 in the Baculoviral Disulfide Bond Formation Pathway. Journal of Virology. 2020; 94 (15):1.
Chicago/Turabian StyleHuanyu Zhang; Wenhua Kuang; Cheng Chen; Yu Shang; Xiaoyan Ma; Fei Deng; Hualin Wang; Manli Wang; Zhihong Hu. 2020. "Per Os Infectivity Factor 5 Identified as a Substrate of P33 in the Baculoviral Disulfide Bond Formation Pathway." Journal of Virology 94, no. 15: 1.
Segmented negative-sense RNA viruses (sNSRVs) encode a single-polypeptide polymerase (L protein) or a heterotrimeric polymerase complex to cannibalize host messenger RNA cap structures serving as primers of transcription, and catalyse RNA synthesis. Here, we report the full-length structure of the severe fever with thrombocytopaenia syndrome virus (SFTSV) L protein, as determined by cryogenic electron microscopy at 3.4 Å, leading to an atomic model harbouring three functional parts (an endonuclease, an RNA-dependent RNA polymerase and a cap-binding domain) and two structural domains (an arm domain with a blocker motif and a carboxy-terminal lariat domain). The SFTSV L protein has a compact architecture in which its cap-binding pocket is surprisingly occupied by an Arg finger of the blocker motif, and the endonuclease active centre faces back towards the cap-binding pocket, suggesting that domain rearrangements are necessary to acquire the pre-initiation state of the active site. Our results provide insight into the complete architecture of sNSRV-encoded L protein and further the understanding of sNSRV transcription initiation.
Panpan Wang; Lu Liu; Aijun Liu; Liming Yan; Yong He; Shu Shen; Mingxu Hu; Yu Guo; Haiguang Liu; Chuang Liu; Yinying Lu; Peiyi Wang; Fei Deng; Zihe Rao; Zhiyong Lou. Structure of severe fever with thrombocytopenia syndrome virus L protein elucidates the mechanisms of viral transcription initiation. Nature Microbiology 2020, 5, 864 -871.
AMA StylePanpan Wang, Lu Liu, Aijun Liu, Liming Yan, Yong He, Shu Shen, Mingxu Hu, Yu Guo, Haiguang Liu, Chuang Liu, Yinying Lu, Peiyi Wang, Fei Deng, Zihe Rao, Zhiyong Lou. Structure of severe fever with thrombocytopenia syndrome virus L protein elucidates the mechanisms of viral transcription initiation. Nature Microbiology. 2020; 5 (6):864-871.
Chicago/Turabian StylePanpan Wang; Lu Liu; Aijun Liu; Liming Yan; Yong He; Shu Shen; Mingxu Hu; Yu Guo; Haiguang Liu; Chuang Liu; Yinying Lu; Peiyi Wang; Fei Deng; Zihe Rao; Zhiyong Lou. 2020. "Structure of severe fever with thrombocytopenia syndrome virus L protein elucidates the mechanisms of viral transcription initiation." Nature Microbiology 5, no. 6: 864-871.
TER94 constitutes an important AAA+ ATPase that associates with diverse cellular processes, including protein quality control, membrane fusion of the Golgi apparatus and endoplasmic reticulum network, nuclear envelope reformation, and DNA replication. To date, little is known regarding the role(s) of TER94 in the baculovirus life cycle. In this study, TER94 was found to play a crucial role in multiple steps of baculovirus infection, including viral DNA replication and BV and ODV formation. Further evidence showed that the membrane fission/fusion function of TER94 is likely to be exploited by baculovirus for virion morphogenesis. Moreover, TER94 could interact with the viral early proteins LEF3 and helicase to transport and further recruit viral replication-related proteins to establish viral replication factories. This study highlights the critical roles of TER94 as an energy-supplying chaperon in the baculovirus life cycle and enriches our knowledge regarding the biological function of this important host factor.
Yimeng Li; Liangbo Hu; Tong Chen; Meng Chang; Fei Deng; Zhihong Hu; Hualin Wang; Manli Wang. Host AAA+ ATPase TER94 Plays Critical Roles in Building the Baculovirus Viral Replication Factory and Virion Morphogenesis. Journal of Virology 2020, 94, 1 .
AMA StyleYimeng Li, Liangbo Hu, Tong Chen, Meng Chang, Fei Deng, Zhihong Hu, Hualin Wang, Manli Wang. Host AAA+ ATPase TER94 Plays Critical Roles in Building the Baculovirus Viral Replication Factory and Virion Morphogenesis. Journal of Virology. 2020; 94 (6):1.
Chicago/Turabian StyleYimeng Li; Liangbo Hu; Tong Chen; Meng Chang; Fei Deng; Zhihong Hu; Hualin Wang; Manli Wang. 2020. "Host AAA+ ATPase TER94 Plays Critical Roles in Building the Baculovirus Viral Replication Factory and Virion Morphogenesis." Journal of Virology 94, no. 6: 1.
Since the SARS outbreak 18 years ago, a large number of severe acute respiratory syndrome related coronaviruses (SARSr-CoV) have been discovered in their natural reservoir host, bats1-4. Previous studies indicated that some of those bat SARSr-CoVs have the potential to infect humans5-7. Here we report the identification and characterization of a novel coronavirus (nCoV-2019) which caused an epidemic of acute respiratory syndrome in humans, in Wuhan, China. The epidemic, started from December 12th, 2019, has caused 198 laboratory confirmed infections with three fatal cases by January 20th, 2020. Full-length genome sequences were obtained from five patients at the early stage of the outbreak. They are almost identical to each other and share 79.5% sequence identify to SARS-CoV. Furthermore, it was found that nCoV-2019 is 96% identical at the whole genome level to a bat coronavirus. The pairwise protein sequence analysis of seven conserved non-structural proteins show that this virus belongs to the species of SARSr-CoV. The nCoV-2019 virus was then isolated from the bronchoalveolar lavage fluid of a critically ill patient, which can be neutralized by sera from several patients. Importantly, we have confirmed that this novel CoV uses the same cell entry receptor, ACE2, as SARS-CoV.
Peng Zhou; Xing-Lou Yang; Xian-Guang Wang; Ben Hu; Lei Zhang; Wei Zhang; Hao-Rui Si; Yan Zhu; Bei Li; Chao-Lin Huang; Hui-Dong Chen; Jing Chen; Yun Luo; Hua Guo; Ren-Di Jiang; Mei-Qin Liu; Ying Chen; Xu-Rui Shen; Xi Wang; Xiao-Shuang Zheng; Kai Zhao; Quan-Jiao Chen; Fei Deng; Lin-Lin Liu; Bing Yan; Fa-Xian Zhan; Yan-Yi Wang; Geng-Fu Xiao; Zheng-Li Shi. Discovery of a novel coronavirus associated with the recent pneumonia outbreak in humans and its potential bat origin. 2020, 1 .
AMA StylePeng Zhou, Xing-Lou Yang, Xian-Guang Wang, Ben Hu, Lei Zhang, Wei Zhang, Hao-Rui Si, Yan Zhu, Bei Li, Chao-Lin Huang, Hui-Dong Chen, Jing Chen, Yun Luo, Hua Guo, Ren-Di Jiang, Mei-Qin Liu, Ying Chen, Xu-Rui Shen, Xi Wang, Xiao-Shuang Zheng, Kai Zhao, Quan-Jiao Chen, Fei Deng, Lin-Lin Liu, Bing Yan, Fa-Xian Zhan, Yan-Yi Wang, Geng-Fu Xiao, Zheng-Li Shi. Discovery of a novel coronavirus associated with the recent pneumonia outbreak in humans and its potential bat origin. . 2020; ():1.
Chicago/Turabian StylePeng Zhou; Xing-Lou Yang; Xian-Guang Wang; Ben Hu; Lei Zhang; Wei Zhang; Hao-Rui Si; Yan Zhu; Bei Li; Chao-Lin Huang; Hui-Dong Chen; Jing Chen; Yun Luo; Hua Guo; Ren-Di Jiang; Mei-Qin Liu; Ying Chen; Xu-Rui Shen; Xi Wang; Xiao-Shuang Zheng; Kai Zhao; Quan-Jiao Chen; Fei Deng; Lin-Lin Liu; Bing Yan; Fa-Xian Zhan; Yan-Yi Wang; Geng-Fu Xiao; Zheng-Li Shi. 2020. "Discovery of a novel coronavirus associated with the recent pneumonia outbreak in humans and its potential bat origin." , no. : 1.