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Mohammad Azam Ansari
Department of Epidemic Disease Research, Institute for Research & Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, P.O. Box 1982, Dammam, 31441, Saudi Arabia

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Original research
Published: 01 August 2021 in International Journal of Nanomedicine
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Purpose: 4-Hydroxyisophthalic acid (4-HIA) is a bioactive compound present in the roots of Decalepis hamiltonii, which has attracted considerable attention in attenuating oxidative stress-related neurodegenerative diseases. However, its efficacy is limited because of its low solubility and bioavailability. Therefore, the present study aimed to encapsulate 4-HIA using biocompatible copolymer polylactide-co-glycolide (PLGA) and evaluate its antioxidant and neuroprotective potential. Methods: The nanoparticles (NPs) were fabricated by solid/oil/water (s/o/w) emulsion technique and characterized using XRD, SEM, HR-TEM, and FTIR spectroscopy. Antioxidant assays such as 1,1 diphenyl-2-picrylhydrazyl (DPPH), superoxide, and hydroxyl radical scavenging ability were performed to assess the antioxidant potential of the fabricated NPs. Results: The bioactive component, 4-HIA, was efficiently encapsulated by the PLGA polymer and was found to be spherical and smooth with a size 2O2)-induced cytotoxicity in PC12 cells. Conclusion: Our findings indicate that 4-HIA encapsulated PLGA NPs might be a therapeutic intervention towards the effective management of oxidative stress as it has exhibited efficient neuroprotective potential against H2O2-induced oxidative stress in PC12 cells.

ACS Style

Tekupalli Ravikiran; Santosh Anand; Mohammad Azam Ansari; Mohammad N Alomary; Sami AlYahya; Sowbhagya Ramachandregowda; Saad Alghamdi; Anupama Sindhghatta Kariyappa; Bhagyalakshmi Dundaiah; Mamatha Madhugiri Gopinath; Sumreen Sultana; Shital Manohar Punekar; Thimappa Ramachandrappa Lakshmeesha. Fabrication and in vitro Evaluation of 4-HIA Encapsulated PLGA Nanoparticles on PC12 Cells. International Journal of Nanomedicine 2021, ume 16, 5621 -5632.

AMA Style

Tekupalli Ravikiran, Santosh Anand, Mohammad Azam Ansari, Mohammad N Alomary, Sami AlYahya, Sowbhagya Ramachandregowda, Saad Alghamdi, Anupama Sindhghatta Kariyappa, Bhagyalakshmi Dundaiah, Mamatha Madhugiri Gopinath, Sumreen Sultana, Shital Manohar Punekar, Thimappa Ramachandrappa Lakshmeesha. Fabrication and in vitro Evaluation of 4-HIA Encapsulated PLGA Nanoparticles on PC12 Cells. International Journal of Nanomedicine. 2021; ume 16 ():5621-5632.

Chicago/Turabian Style

Tekupalli Ravikiran; Santosh Anand; Mohammad Azam Ansari; Mohammad N Alomary; Sami AlYahya; Sowbhagya Ramachandregowda; Saad Alghamdi; Anupama Sindhghatta Kariyappa; Bhagyalakshmi Dundaiah; Mamatha Madhugiri Gopinath; Sumreen Sultana; Shital Manohar Punekar; Thimappa Ramachandrappa Lakshmeesha. 2021. "Fabrication and in vitro Evaluation of 4-HIA Encapsulated PLGA Nanoparticles on PC12 Cells." International Journal of Nanomedicine ume 16, no. : 5621-5632.

Journal article
Published: 29 July 2021 in Journal of Saudi Chemical Society
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Colorectal cancer is one of the most common cancers worldwide, and it is also one of the major causes of mortality from cancer. Chemotherapy drugs are generally limited due to various complications, as well as the development of resistance and recurrence. The in silico docking investigation involved exploration of protein or nucleotide, 3D structural modeling, molecular docking, and binding energy calculation. Protein-protein interactions are significant to many biological processes, and their disruption is a leading cause of disease. The use of small molecules to modulate them is gaining popularity, but protein interfaces usually lack specific cavities for processing small molecules. MMP-2, PARP, iNOS, Chk1, proteins were used in the molecular docking analysis of kaempferitrin and 5-flurouracil. The compound kaempferitrin had the highest binding energy scores with most of the target proteins, according to molecular docking results. The findings suggest it could be used to develop new drugs for cancer therapy.

ACS Style

Mydhili Govindarasu; Shalini Ganeshan; Mohammad Azam Ansari; Mohammad N. Alomary; Sami AlYahya; Saad Alghamdi; Mazen Almehmadi; Govindasamy Rajakumar; Muthu Thiruvengadam; Manju Vaiyapuri. In silico modeling and molecular docking insights of kaempferitrin for colon cancer-related molecular targets. Journal of Saudi Chemical Society 2021, 25, 101319 .

AMA Style

Mydhili Govindarasu, Shalini Ganeshan, Mohammad Azam Ansari, Mohammad N. Alomary, Sami AlYahya, Saad Alghamdi, Mazen Almehmadi, Govindasamy Rajakumar, Muthu Thiruvengadam, Manju Vaiyapuri. In silico modeling and molecular docking insights of kaempferitrin for colon cancer-related molecular targets. Journal of Saudi Chemical Society. 2021; 25 (9):101319.

Chicago/Turabian Style

Mydhili Govindarasu; Shalini Ganeshan; Mohammad Azam Ansari; Mohammad N. Alomary; Sami AlYahya; Saad Alghamdi; Mazen Almehmadi; Govindasamy Rajakumar; Muthu Thiruvengadam; Manju Vaiyapuri. 2021. "In silico modeling and molecular docking insights of kaempferitrin for colon cancer-related molecular targets." Journal of Saudi Chemical Society 25, no. 9: 101319.

Journal article
Published: 16 June 2021 in Antibiotics
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Biofilms not only protect bacteria and Candida species from antibiotics, but they also promote the emergence of drug-resistant strains, making eradication more challenging. As a result, novel antimicrobial agents to counteract biofilm formation are desperately needed. In this study, Terminalia catappa leaf extract (TCE) was used to optimize the TCE-capped silver nanoparticles (TCE-AgNPs) via a one-pot single-step method. Varied concentrations of TCE have yielded different sized AgNPs. The physico-chemical characterization of TCE-AgNPs using UV-Vis, SEM, TEM, FTIR, and Raman spectroscopy have confirmed the formation of nanostructures, their shape and size and plausible role of TCE bio-active compounds, most likely involved in the synthesis as well as stabilization of NPs, respectively. TCE-AgNPs have been tested for antibiofilm and antimicrobial activity against multidrug-resistant Pseudomonas aeruginosa (MDR-PA), methicillin-resistant Staphylococcus aureus (MRSA), and Candida albicans using various microbiological protocols. TCE-Ag-NPs−3 significantly inhibits biofilm formation of MDR-PA, MRSA, and C. albicans by 73.7, 69.56, and 63.63%, respectively, at a concentration of 7.8 µg/mL, as determined by crystal violet microtiter assay. Furthermore, SEM micrograph shows that TCE-AgNPs significantly inhibit the colonization and adherence of biofilm forming cells; individual cells with loss of cell wall and membrane integrity were also observed, suggesting that the biofilm architecture and EPS matrix were severely damaged. Moreover, TEM and SEM images showed that TCE-AgNPs brutally damaged the cell wall and membranes of MDR-PA, MRSA, and C. albicans. Additionally, extreme ultrastructural changes such as deformation, disintegration, and separation of cell wall and membrane from the cells, have also been observed, indicating significant loss of membrane and cell wall integrity, which eventually led to cell death. Overall, the research revealed a simple, environmentally friendly, and low-cost method for producing colloidal TCE-AgNPs with promising applications in advanced clinical settings against broad-spectrum biofilm-forming antibiotic-resistant bacteria and candida strains.

ACS Style

Mohammad Ansari; Abul Kalam; Abdullah Al-Sehemi; Mohammad Alomary; Sami AlYahya; Mohammad Aziz; Shekhar Srivastava; Saad Alghamdi; Sultan Akhtar; Hussain Almalki; Syed Adil; Mujeeb Khan; Mohammad Hatshan. Counteraction of Biofilm Formation and Antimicrobial Potential of Terminalia catappa Functionalized Silver Nanoparticles against Candida albicans and Multidrug-Resistant Gram-Negative and Gram-Positive Bacteria. Antibiotics 2021, 10, 725 .

AMA Style

Mohammad Ansari, Abul Kalam, Abdullah Al-Sehemi, Mohammad Alomary, Sami AlYahya, Mohammad Aziz, Shekhar Srivastava, Saad Alghamdi, Sultan Akhtar, Hussain Almalki, Syed Adil, Mujeeb Khan, Mohammad Hatshan. Counteraction of Biofilm Formation and Antimicrobial Potential of Terminalia catappa Functionalized Silver Nanoparticles against Candida albicans and Multidrug-Resistant Gram-Negative and Gram-Positive Bacteria. Antibiotics. 2021; 10 (6):725.

Chicago/Turabian Style

Mohammad Ansari; Abul Kalam; Abdullah Al-Sehemi; Mohammad Alomary; Sami AlYahya; Mohammad Aziz; Shekhar Srivastava; Saad Alghamdi; Sultan Akhtar; Hussain Almalki; Syed Adil; Mujeeb Khan; Mohammad Hatshan. 2021. "Counteraction of Biofilm Formation and Antimicrobial Potential of Terminalia catappa Functionalized Silver Nanoparticles against Candida albicans and Multidrug-Resistant Gram-Negative and Gram-Positive Bacteria." Antibiotics 10, no. 6: 725.

Journal article
Published: 28 April 2021 in Saudi Journal of Biological Sciences
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The human-to-human transmitted respiratory illness in COVID-19 affected by the pathogenic Severe Acute Respiratory Syndrome Corona Virus 2 (SARS-CoV-2), which appeared in the last of December 2019 in Wuhan, China, and rapidly spread in many countries. Thereon, based on the urgent need for therapeutic molecules, we conducted in silico based docking and simulation molecular interaction studies on repurposing drugs, targeting SARS-CoV-2 spike protein. Further, the best binding energy of doxorubicin interacting with virus spike protein (PDB: 6VYB) was observed to be −6.38 kcal/mol and it was followed by exemestane and gatifloxacin. The molecular simulation dynamics analysis of doxorubicin, Reference Mean Square Deviation (RMSD), Root Mean Square fluctuation (RMSF), Radius of Gyration (Rg), and formation of hydrogen bonds plot interpretation suggested, a significant deviation and fluctuation of Doxorubicin-Spike RBD complex during the whole simulation period. The Rg analysis has stated that the Doxorubicin-Spike RBD complex was stable during 15,000–35,000 ps MDS. The results have suggested that doxorubicin could inhibit the virus spike protein and prevent the access of the SARS-CoV-2 to the host cell. Thus, in-vitro/in-vivo research on these drugs could be advantageous to evaluate significant molecules that control the COVID-19 disease.

ACS Style

Qazi Mohammad Sajid Jamal; Varish Ahmad; Ali H Alharbi; Mohammad Azam Ansari; Mohammad A Alzohairy; Ahmad Almatroudi; Saad Alghamdi; Mohammad N. Alomary; Sami AlYahya; Nashwa Talaat Shesha; Suriya Rehman. Therapeutic development by repurposing drugs targeting SARS-CoV-2 spike protein interactions by simulation studies. Saudi Journal of Biological Sciences 2021, 1 .

AMA Style

Qazi Mohammad Sajid Jamal, Varish Ahmad, Ali H Alharbi, Mohammad Azam Ansari, Mohammad A Alzohairy, Ahmad Almatroudi, Saad Alghamdi, Mohammad N. Alomary, Sami AlYahya, Nashwa Talaat Shesha, Suriya Rehman. Therapeutic development by repurposing drugs targeting SARS-CoV-2 spike protein interactions by simulation studies. Saudi Journal of Biological Sciences. 2021; ():1.

Chicago/Turabian Style

Qazi Mohammad Sajid Jamal; Varish Ahmad; Ali H Alharbi; Mohammad Azam Ansari; Mohammad A Alzohairy; Ahmad Almatroudi; Saad Alghamdi; Mohammad N. Alomary; Sami AlYahya; Nashwa Talaat Shesha; Suriya Rehman. 2021. "Therapeutic development by repurposing drugs targeting SARS-CoV-2 spike protein interactions by simulation studies." Saudi Journal of Biological Sciences , no. : 1.

Review
Published: 18 April 2021 in Journal of Fungi
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Rhizosphere-resident fungi that are helpful to plants are generally termed as ‘plant growth promoting fungi’ (PGPF). These fungi are one of the chief sources of the biotic inducers known to give their host plants numerous advantages, and they play a vital role in sustainable agriculture. Today’s biggest challenge is to satisfy the rising demand for crop protection and crop yield without harming the natural ecosystem. Nowadays, PGPF has become an eco-friendly way to improve crop yield by enhancing seed germination, shoot and root growth, chlorophyll production, and fruit yield, etc., either directly or indirectly. The mode of action of these PGPF includes the solubilization and mineralization of the essential micro- and macronutrients needed by plants to regulate the balance for various plant processes. PGPF produce defense-related enzymes, defensive/volatile compounds, and phytohormones that control pathogenic microbes’ growth, thereby assisting the plants in facing various biotic and abiotic stresses. Therefore, this review presents a holistic view of PGPF as efficient natural biofertilizers to improve crop plants’ growth and resistance.

ACS Style

Mahadevamurthy Murali; Banu Naziya; Mohammad Ansari; Mohammad Alomary; Sami AlYahya; Ahmad Almatroudi; M. Thriveni; Hittanahallikoppal Gowtham; Sudarshana Singh; Mohammed Aiyaz; Nataraj Kalegowda; Nanjaiah Lakshmidevi; Kestur Amruthesh. Bioprospecting of Rhizosphere-Resident Fungi: Their Role and Importance in Sustainable Agriculture. Journal of Fungi 2021, 7, 314 .

AMA Style

Mahadevamurthy Murali, Banu Naziya, Mohammad Ansari, Mohammad Alomary, Sami AlYahya, Ahmad Almatroudi, M. Thriveni, Hittanahallikoppal Gowtham, Sudarshana Singh, Mohammed Aiyaz, Nataraj Kalegowda, Nanjaiah Lakshmidevi, Kestur Amruthesh. Bioprospecting of Rhizosphere-Resident Fungi: Their Role and Importance in Sustainable Agriculture. Journal of Fungi. 2021; 7 (4):314.

Chicago/Turabian Style

Mahadevamurthy Murali; Banu Naziya; Mohammad Ansari; Mohammad Alomary; Sami AlYahya; Ahmad Almatroudi; M. Thriveni; Hittanahallikoppal Gowtham; Sudarshana Singh; Mohammed Aiyaz; Nataraj Kalegowda; Nanjaiah Lakshmidevi; Kestur Amruthesh. 2021. "Bioprospecting of Rhizosphere-Resident Fungi: Their Role and Importance in Sustainable Agriculture." Journal of Fungi 7, no. 4: 314.

Journal article
Published: 03 March 2021 in Future Oncology
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Lung cancer is generally diagnosed at advanced stages when surgical resection is not possible. Late diagnosis, along with development of chemoresistance, results in high mortality. Preventive approaches, including smoking cessation, chemoprevention and early detection are needed to improve survival. Smoking cessation combined with low-dose computed tomography screening has modestly improved survival. Chemoprevention has also shown some promise. Despite these successes, most lung cancer cases remain undetected until advanced stages. Additional early detection strategies may further improve survival and treatment outcome. Molecular alterations taking place during lung carcinogenesis have the potential to be used in early detection via noninvasive methods and may also serve as biomarkers for success of chemopreventive approaches. This review focuses on the utilization of molecular biomarkers to increase the efficacy of various preventive approaches.

ACS Style

Asrar Alam; Mohammad A Ansari; Khan F Badrealam; Sujata Pathak. Molecular approaches to lung cancer prevention. Future Oncology 2021, 1 .

AMA Style

Asrar Alam, Mohammad A Ansari, Khan F Badrealam, Sujata Pathak. Molecular approaches to lung cancer prevention. Future Oncology. 2021; ():1.

Chicago/Turabian Style

Asrar Alam; Mohammad A Ansari; Khan F Badrealam; Sujata Pathak. 2021. "Molecular approaches to lung cancer prevention." Future Oncology , no. : 1.

Review
Published: 14 February 2021 in Medicina
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Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has caused a global pandemic and is posing a serious challenge to mankind. As per the current scenario, there is an urgent need for antiviral that could act as a protective and therapeutic against SARS-CoV-2. Previous studies have shown that SARS-CoV-2 is much similar to the SARS-CoV bat that occurred in 2002-03. Since it is a zoonotic virus, the exact source is still unknown, but it is believed bats may be the primary reservoir of SARS-CoV-2 through which it has been transferred to humans. In this review, we have tried to summarize some of the approaches that could be effective against SARS-CoV-2. Firstly, plants or plant-based products have been effective against different viral diseases, and secondly, plants or plant-based natural products have the minimum adverse effect. We have also highlighted a few vitamins and minerals that could be beneficial against SARS-CoV-2.

ACS Style

Syed Ali; Mohammad Ansari; Mohammad Alzohairy; Ahmad Almatroudi; Mohammad Alomary; Saad Alghamdi; Suriya Rehman; Haris Khan. Natural Products and Nutrients against Different Viral Diseases: Prospects in Prevention and Treatment of SARS-CoV-2. Medicina 2021, 57, 169 .

AMA Style

Syed Ali, Mohammad Ansari, Mohammad Alzohairy, Ahmad Almatroudi, Mohammad Alomary, Saad Alghamdi, Suriya Rehman, Haris Khan. Natural Products and Nutrients against Different Viral Diseases: Prospects in Prevention and Treatment of SARS-CoV-2. Medicina. 2021; 57 (2):169.

Chicago/Turabian Style

Syed Ali; Mohammad Ansari; Mohammad Alzohairy; Ahmad Almatroudi; Mohammad Alomary; Saad Alghamdi; Suriya Rehman; Haris Khan. 2021. "Natural Products and Nutrients against Different Viral Diseases: Prospects in Prevention and Treatment of SARS-CoV-2." Medicina 57, no. 2: 169.

Journal article
Published: 09 February 2021 in Pharmaceuticals
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The current study demonstrates the synthesis of fatty acids (FAs) capped silver nanoparticles (AgNPs) using aqueous poly-herbal drug Liv52 extract (PLE) as a reducing, dispersing and stabilizing agent. The NPs were characterized by various techniques and used to investigate their potent antibacterial, antibiofilm, antifungal and anticancer activities. GC-MS analysis of PLE shows a total of 37 peaks for a variety of bio-actives compounds. Amongst them, n-hexadecanoic acid (21.95%), linoleic acid (20.45%), oleic acid (18.01%) and stearic acid (13.99%) were found predominately and most likely acted as reducing, stabilizing and encapsulation FAs in LIV-AgNPs formation. FTIR analysis of LIV-AgNPs shows some other functional bio-actives like proteins, sugars and alkenes in the soft PLE corona. The zone of inhibition was 10.0 ± 2.2–18.5 ± 1.0 mm, 10.5 ± 2.5–22.5 ± 1.5 mm and 13.7 ± 1.0–16.5 ± 1.2 against P. aeruginosa, S. aureus and C. albicans, respectively. LIV-AgNPs inhibit biofilm formation in a dose-dependent manner i.e., 54.4% ± 3.1%—10.12% ± 2.3% (S. aureus), 72.7% ± 2.2%–23.3% ± 5.2% (P. aeruginosa) and 85.4% ± 3.3%–25.6% ± 2.2% (C. albicans), and SEM analysis of treated planktonic cells and their biofilm biomass validated the fitness of LIV-AgNPs in future nanoantibiotics. In addition, as prepared FAs rich PLE capped AgNPs have also exhibited significant (p < 0.05 *) antiproliferative activity against cultured HCT-116 cells. Overall, this is a very first demonstration on employment of FAs rich PLE for the synthesis of highly dispersible, stable and uniform sized AgNPs and their antibacterial, antifungal, antibiofilm and anticancer efficacy.

ACS Style

Mohammad Ansari; Sarah Asiri; Mohammad Alzohairy; Mohammad Alomary; Ahmad Almatroudi; Firdos Khan. Biofabricated Fatty Acids-Capped Silver Nanoparticles as Potential Antibacterial, Antifungal, Antibiofilm and Anticancer Agents. Pharmaceuticals 2021, 14, 139 .

AMA Style

Mohammad Ansari, Sarah Asiri, Mohammad Alzohairy, Mohammad Alomary, Ahmad Almatroudi, Firdos Khan. Biofabricated Fatty Acids-Capped Silver Nanoparticles as Potential Antibacterial, Antifungal, Antibiofilm and Anticancer Agents. Pharmaceuticals. 2021; 14 (2):139.

Chicago/Turabian Style

Mohammad Ansari; Sarah Asiri; Mohammad Alzohairy; Mohammad Alomary; Ahmad Almatroudi; Firdos Khan. 2021. "Biofabricated Fatty Acids-Capped Silver Nanoparticles as Potential Antibacterial, Antifungal, Antibiofilm and Anticancer Agents." Pharmaceuticals 14, no. 2: 139.

Journal article
Published: 08 February 2021 in Molecules
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The study was undertaken to investigate the antioxidant, genotoxic, and cytotoxic potentialities of phyto-fabricated zinc oxide nanoparticles (ZnO-NPs) from Ipomoea obscura (L.) Ker Gawl. aqueous leaf extract. The UV-visible spectral analysis of the ZnO-NPs showed an absorption peak at 304 nm with a bandgap energy of 3.54 eV, which are characteristics of zinc nanoparticles. Moreover, the particles were of nano-size (~24.26 nm) with 88.11% purity and were agglomerated as observed through Scanning Electron Microscopy (SEM). The phyto-fabricated ZnO-NPs offered radical scavenging activity (RSA) in a dose-dependent manner with an IC50 of 0.45 mg mL−1. In addition, the genotoxicity studies of ZnO-NPs carried out on onion root tips revealed that the particles were able to significantly inhibit the cell division at the mitotic stage with a mitotic index of 39.49%. Further, the cytotoxic studies on HT-29 cells showed that the phyto-fabricated ZnO-NPs could arrest the cell division as early as in the G0/G1 phase (with 92.14%) with 73.14% cells showing early apoptotic symptoms after 24 h of incubation. The results of the study affirm the ability of phyto-fabricated ZnO-NPs from aqueous leaf extract of I. obscura is beneficial in the cytotoxic application.

ACS Style

Mahadevamurthy Murali; Satish Anandan; Mohammad Ansari; Mohammad Alzohairy; Mohammad Alomary; Sarah Asiri; Ahmad Almatroudi; M. Thriveni; Sudarshana Singh; Hittanahallikoppal Gowtham; Mohammed Aiyaz; Chandrashekar Srinivasa; Asna Urooj; Kestur Amruthesh. Genotoxic and Cytotoxic Properties of Zinc Oxide Nanoparticles Phyto-Fabricated from the Obscure Morning Glory Plant Ipomoea obscura (L.) Ker Gawl. Molecules 2021, 26, 891 .

AMA Style

Mahadevamurthy Murali, Satish Anandan, Mohammad Ansari, Mohammad Alzohairy, Mohammad Alomary, Sarah Asiri, Ahmad Almatroudi, M. Thriveni, Sudarshana Singh, Hittanahallikoppal Gowtham, Mohammed Aiyaz, Chandrashekar Srinivasa, Asna Urooj, Kestur Amruthesh. Genotoxic and Cytotoxic Properties of Zinc Oxide Nanoparticles Phyto-Fabricated from the Obscure Morning Glory Plant Ipomoea obscura (L.) Ker Gawl. Molecules. 2021; 26 (4):891.

Chicago/Turabian Style

Mahadevamurthy Murali; Satish Anandan; Mohammad Ansari; Mohammad Alzohairy; Mohammad Alomary; Sarah Asiri; Ahmad Almatroudi; M. Thriveni; Sudarshana Singh; Hittanahallikoppal Gowtham; Mohammed Aiyaz; Chandrashekar Srinivasa; Asna Urooj; Kestur Amruthesh. 2021. "Genotoxic and Cytotoxic Properties of Zinc Oxide Nanoparticles Phyto-Fabricated from the Obscure Morning Glory Plant Ipomoea obscura (L.) Ker Gawl." Molecules 26, no. 4: 891.

Full paper
Published: 12 January 2021 in Chemistry - A European Journal
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The biofunctionalized TiO2 NPs, having a size range of 18.42 ± 1.3 nm were synthesized employing single step approach employing Grape seed extract (GSE) mediated proanthocyanins (PACs) polyphenols. The present study purposes to examine the effect of PACs rich GSE corona on (i) the stability and dispersity of GSE‐TiO 2 ‐NPs, their (ii) anti‐proliferative and anti‐biofilm efficacy and (iii) propensity of internalization and reactive oxygen species (ROS) generation in P. aeruginosa and S. saprophyticus strains. The state‐of‐the‐art techniques were used to validate NPs formation. The comparative FTIR spectral analysis demonstrated that PACs linked functional ‐OH groups likely played a central role in Ti 4+ reduction, nucleation, formation and stability of GSE‐TiO 2 ‐NPs. The TEM and ICP‐MS analyses confirmed there was significantly (p < 0.05) enhanced intracellular uptake of GSE‐TiO 2 ‐NPs in tested uropathogens as compare to bare TiO 2 ‐NPs. Correspondingly, compared to bare NPs, GSE‐TiO 2 ‐NPs induced intracellular ROS formation that corresponded well with dose‐dependent inhibitory patterns of cell proliferation and biofilm formation in both the tested strains. Overall, this study demonstrates that ‐OH rich PACs of GSE corona on biogenic TiO 2 ‐NPs maximized the functional stability, dispersity and propensity of penetration into planktonic cells and biofilm matrices; albeit, such unique merits warranted the GSE‐TiO 2 ‐NPs as a novel, functionally stable and efficient antibacterial nanoformulation to combat with UTIs menace in clinical settings

ACS Style

Mohammad N. Alomary; Mohammad Azam Ansari. Proanthocyanin‐Capped Biogenic TiO 2 Nanoparticles with Enhanced Penetration, Antibacterial and ROS Mediated Inhibition of Bacteria Proliferation and Biofilm Formation: A Comparative Approach. Chemistry - A European Journal 2021, 27, 5817 -5829.

AMA Style

Mohammad N. Alomary, Mohammad Azam Ansari. Proanthocyanin‐Capped Biogenic TiO 2 Nanoparticles with Enhanced Penetration, Antibacterial and ROS Mediated Inhibition of Bacteria Proliferation and Biofilm Formation: A Comparative Approach. Chemistry - A European Journal. 2021; 27 (18):5817-5829.

Chicago/Turabian Style

Mohammad N. Alomary; Mohammad Azam Ansari. 2021. "Proanthocyanin‐Capped Biogenic TiO 2 Nanoparticles with Enhanced Penetration, Antibacterial and ROS Mediated Inhibition of Bacteria Proliferation and Biofilm Formation: A Comparative Approach." Chemistry - A European Journal 27, no. 18: 5817-5829.

Journal article
Published: 29 December 2020 in Membranes
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We fabricated a nanofiltration membrane consisting of a polyaniline (PANI) film on a polyphenylsulfone (PPSU) substrate membrane. The PANI film acted as a potent separation enhancer and antimicrobial coating. The membrane was analyzed via scanning electron microscopy and atomic force microscopy to examine its morphology, topography, contact angle, and zeta potential. We aimed to investigate the impact of the PANI film on the surface properties of the membrane. Membrane performance was then evaluated in terms of water permeation and rejection of methylene blue (MB), an organic dye. Coating the PPSU membrane with a PANI film imparted significant advantages, including finely tuned nanometer-scale membrane pores and tailored surface properties, including increased hydrophilicity and zeta potential. The PANI film also significantly enhanced separation of the MB dye. The PANI-coated membrane rejected over 90% of MB with little compromise in membrane permeability. The PANI film also enhanced the antimicrobial activity of the membrane. The bacteriostasis (BR) values of PANI-coated PPSU membranes after six and sixteen hours of incubation with Escherichia coli were 63.5% and 95.2%, respectively. The BR values of PANI-coated PPSU membranes after six and sixteen hours of incubation with Staphylococcus aureus were 70.6% and 88.0%, respectively.

ACS Style

Javed Alam; Arun Shukla; Mohammad Ansari; Fekri Ali; Mansour Alhoshan. Dye Separation and Antibacterial Activities of Polyaniline Thin Film-Coated Poly(phenyl sulfone) Membranes. Membranes 2020, 11, 25 .

AMA Style

Javed Alam, Arun Shukla, Mohammad Ansari, Fekri Ali, Mansour Alhoshan. Dye Separation and Antibacterial Activities of Polyaniline Thin Film-Coated Poly(phenyl sulfone) Membranes. Membranes. 2020; 11 (1):25.

Chicago/Turabian Style

Javed Alam; Arun Shukla; Mohammad Ansari; Fekri Ali; Mansour Alhoshan. 2020. "Dye Separation and Antibacterial Activities of Polyaniline Thin Film-Coated Poly(phenyl sulfone) Membranes." Membranes 11, no. 1: 25.

Research article
Published: 26 December 2020 in International Journal of Biological Macromolecules
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Fatty acids-assisted superparamagnetic maghemite (γ-Fe2O3) NPs was biologically synthesized using extract of polyherbal drug Liv52 (L52E). The NPs were characterized by UV–vis spectroscopy, FT-IR, SEM, TEM, EDX, XRD and VSM. The major biological molecules present in L52E analysed by GC–MS were saturated fatty acids (palmitic acid 21.95%; stearic acid 13.99%; myristic acid 1.14%), monounsaturated fatty acid (oleic acid 18.43%), polyunsaturated fatty acid (linoleic acid 20.45%), and aromatic phenol (cardanol monoene 11.92%) that could imply in bio-fabrication and stabilization of γ-Fe2O3 NPs. The FT-IR spectra revealed involvement of carboxylic group of fatty acids, amide group of proteins and hydroxyl group of phenolic compounds that acts as reducing and capping agents. The synthesized NPs were used to investigate their antimicrobial, antibiofilm activity against P. aeruginosa, MRSA and C. albicans and anticancer activity on colon cancer cells (HCT-116) for biomedical applications. Further, molecular docking study was performed to explore the interaction of Fe2O3 NPs with major cell wall components i.e., peptidoglycan and mannoproteins. The docking studies revealed that Fe2O3 interacted efficiently with peptidoglycan and mannoproteins and Fe2O3 get accommodated into catalytic cleft of mannoprotein. Due to magnetic property, the biological activity of γ-Fe2O3 can be further enhanced by applying external magnetic field alone or in amalgamation with other therapeutics drugs.

ACS Style

Mohammad Azam Ansari; Sarah Mousa Maadi Asiri. Green synthesis, antimicrobial, antibiofilm and antitumor activities of superparamagnetic γ-Fe2O3 NPs and their molecular docking study with cell wall mannoproteins and peptidoglycan. International Journal of Biological Macromolecules 2020, 171, 44 -58.

AMA Style

Mohammad Azam Ansari, Sarah Mousa Maadi Asiri. Green synthesis, antimicrobial, antibiofilm and antitumor activities of superparamagnetic γ-Fe2O3 NPs and their molecular docking study with cell wall mannoproteins and peptidoglycan. International Journal of Biological Macromolecules. 2020; 171 ():44-58.

Chicago/Turabian Style

Mohammad Azam Ansari; Sarah Mousa Maadi Asiri. 2020. "Green synthesis, antimicrobial, antibiofilm and antitumor activities of superparamagnetic γ-Fe2O3 NPs and their molecular docking study with cell wall mannoproteins and peptidoglycan." International Journal of Biological Macromolecules 171, no. : 44-58.

Journal article
Published: 01 October 2020 in Arabian Journal of Chemistry
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The Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that originated in Chinese city of Wuhan has caused around 906,092 deaths and 28,040,853 confirmed cases worldwide (https://covid19.who.int/, 11 September 2020). In a life-threatening situation, where there is no specific and licensed anti-COVID-19 vaccine or medicine available; the repurposed drug might act as a silver bullet. Currently, more than 211 vaccines, 80 antibodies, 31 antiviral drugs, 35 cell-based, 6 RNA-based and 131 other drugs are in clinical trials. It is therefore utter need of the hour to develop an effective drug that can be used for the treatment of COVID-19 before a vaccine can be developed. One of the best-characterized and attractive drug targets among coronaviruses is the main protease (3CLpro). Therefore, the current study focuses on the molecular docking analysis of TAT-peptide47–57 (GRKKRRQRRRP)-conjugated repurposed drugs (i.e., lopinavir, ritonavir, favipiravir, and hydroxychloroquine) with SARS-CoV-2 main protease (3CLpro) to discover potential efficacy of TAT-peptide (TP) - conjugated repurposing drugs against SARS-CoV-2. The molecular docking results validated that TP-conjugated ritonavir, lopinavir, favipiravir, and hydroxychloroquine have superior and significantly enhanced interactions with the target SARS-CoV-2 main protease. In-silico approach employed in this study suggests that the combination of the drug with TP is an excelling alternative to develop a novel drug for the treatment of SARS-CoV-2 infected patients. The development of TP based delivery of repurposing drugs might be an excellent approach to enhance the efficacy of the existing drugs for the treatment of COVID-19. The predictions from the results obtained provide invaluable information that can be utilized for the choice of candidate drugs for in vitro, in vivo and clinical trials. The outcome from this work prove crucial for exploring and developing novel cost-effective and biocompatible TP conjugated anti-SARS-CoV-2 therapeutic agents in immediate future.

ACS Style

Mohammad Azam Ansari; Qazi Mohammad Sajid Jamal; Suriya Rehman; Ahmad Almatroudi; Mohammad A. Alzohairy; Mohammad N. Alomary; Takshashila Tripathi; Ali H. Alharbi; Syed Farooq Adil; Mujeeb Khan; M. Shaheer Malik. TAT-peptide conjugated repurposing drug against SARS-CoV-2 main protease (3CLpro): Potential therapeutic intervention to combat COVID-19. Arabian Journal of Chemistry 2020, 13, 8069 -8079.

AMA Style

Mohammad Azam Ansari, Qazi Mohammad Sajid Jamal, Suriya Rehman, Ahmad Almatroudi, Mohammad A. Alzohairy, Mohammad N. Alomary, Takshashila Tripathi, Ali H. Alharbi, Syed Farooq Adil, Mujeeb Khan, M. Shaheer Malik. TAT-peptide conjugated repurposing drug against SARS-CoV-2 main protease (3CLpro): Potential therapeutic intervention to combat COVID-19. Arabian Journal of Chemistry. 2020; 13 (11):8069-8079.

Chicago/Turabian Style

Mohammad Azam Ansari; Qazi Mohammad Sajid Jamal; Suriya Rehman; Ahmad Almatroudi; Mohammad A. Alzohairy; Mohammad N. Alomary; Takshashila Tripathi; Ali H. Alharbi; Syed Farooq Adil; Mujeeb Khan; M. Shaheer Malik. 2020. "TAT-peptide conjugated repurposing drug against SARS-CoV-2 main protease (3CLpro): Potential therapeutic intervention to combat COVID-19." Arabian Journal of Chemistry 13, no. 11: 8069-8079.

Chapter
Published: 26 September 2020 in Smart and Sustainable Planning for Cities and Regions
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Increasing water scarcity and diminishing freshwater resources have generated the need for new water filtration technologies to supply safe potable water for domestic and industrial uses. Filtration with polymeric membranes shows good capability for water treatment; however, it has some limitations, such as selectivity and permeability, and fouling, thereby, limits the continuity of safe water. Long-term filtration stability can be improved using a new generation of nanocomposite membranes that are formed by incorporating nanomaterials into polymeric membrane matrices. A number of different nanomaterials and methods have been adopted at an accelerated pace to develop a thin-film nanocomposite (TFN) membrane. Among these nanomaterials, graphene and its functionalized derivatives have received much research attention, as this approach for developing a new TFN membrane has shown excellent performance, leading to water treatment. Graphene has superior properties that are highly advantageous for solving major problems, such as biofouling, scaling, low flux rate, selectivity, and degradation. This chapter provides an overview of the development of graphene-based nanocomposite membrane methods and physicochemical properties after the incorporation of graphene nanomaterials and their derivatives. A specific focus has been employed on improving understanding of how graphene nanomaterials can be used in a number of different ways, such as ultrafiltration, nanofiltration, and reverse osmosis for water filtration.

ACS Style

Arun Kumar Shukla; Mohammad Azam Ansari; Javed Alam; Ali Aldalbahi; Mansour Alhoshan. Recent Advances in Preparation and Characterization of Graphene-Based Nanocomposite Membranes for Water Purification. Smart and Sustainable Planning for Cities and Regions 2020, 403 -425.

AMA Style

Arun Kumar Shukla, Mohammad Azam Ansari, Javed Alam, Ali Aldalbahi, Mansour Alhoshan. Recent Advances in Preparation and Characterization of Graphene-Based Nanocomposite Membranes for Water Purification. Smart and Sustainable Planning for Cities and Regions. 2020; ():403-425.

Chicago/Turabian Style

Arun Kumar Shukla; Mohammad Azam Ansari; Javed Alam; Ali Aldalbahi; Mansour Alhoshan. 2020. "Recent Advances in Preparation and Characterization of Graphene-Based Nanocomposite Membranes for Water Purification." Smart and Sustainable Planning for Cities and Regions , no. : 403-425.

Journal article
Published: 20 September 2020 in Journal of Fungi
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The Plant Growth Promoting Fungi (PGPF) is used as a source of biofertilizers due to their production of secondary metabolites and beneficial effects on plants. The present work is focused on the co-cultivation of Trichoderma spp. (T. harzianum (PGT4), T. reesei (PGT5) and T. reesei (PGT13)) and the production of secondary metabolites from mono and co-culture and mycosynthesis of zinc oxide nanoparticles (ZnO NPs), which were characterized by a UV visible spectrophotometer, Powder X-ray Diffraction (PXRD), Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscopy (SEM) with Energy Dispersive Spectroscopy (EDAX) and Transmission Electron Microscope (TEM) and Selected Area (Electron) Diffraction (SAED) patterns. The fungal secondary metabolite crude was extracted from the mono and co-culture of Trichoderma spp. And were analyzed by GC-MS, which was further subjected for antibacterial activity against Xanthomonas oryzae pv. Oryzae, the causative organism for Bacterial Leaf Blight (BLB) in rice. Our results showed that the maximum zone of inhibition was recorded from the co-culture of Trichoderma spp. rather than mono cultures, which indicates that co-cultivation of beneficial fungi can stimulate the synthesis of novel secondary metabolites better than in monocultures. ZnO NPs were synthesized from fungal secondary metabolites of mono cultures of Trichoderma harzianum (PGT4), Trichoderma reesei (PGT5), Trichoderma reesei (PGT13) and co-culture (PGT4 + PGT5 + PGT13). These ZnO NPs were checked for antibacterial activity against Xoo, which was found to be of a dose-dependent manner. In summary, the biosynthesized ZnO NPs and secondary metabolites from co-culture of Trichoderma spp. are ecofriendly and can be used as an alternative for chemical fertilizers in agriculture.

ACS Style

Balagangadharaswamy Shobha; Thimappa Lakshmeesha; Mohammad Ansari; Ahmad Almatroudi; Mohammad Alzohairy; Sumanth Basavaraju; Ramesha Alurappa; Siddapura Niranjana; Srinivas Chowdappa. Mycosynthesis of ZnO Nanoparticles Using Trichoderma spp. Isolated from Rhizosphere Soils and Its Synergistic Antibacterial Effect against Xanthomonas oryzae pv. oryzae. Journal of Fungi 2020, 6, 181 .

AMA Style

Balagangadharaswamy Shobha, Thimappa Lakshmeesha, Mohammad Ansari, Ahmad Almatroudi, Mohammad Alzohairy, Sumanth Basavaraju, Ramesha Alurappa, Siddapura Niranjana, Srinivas Chowdappa. Mycosynthesis of ZnO Nanoparticles Using Trichoderma spp. Isolated from Rhizosphere Soils and Its Synergistic Antibacterial Effect against Xanthomonas oryzae pv. oryzae. Journal of Fungi. 2020; 6 (3):181.

Chicago/Turabian Style

Balagangadharaswamy Shobha; Thimappa Lakshmeesha; Mohammad Ansari; Ahmad Almatroudi; Mohammad Alzohairy; Sumanth Basavaraju; Ramesha Alurappa; Siddapura Niranjana; Srinivas Chowdappa. 2020. "Mycosynthesis of ZnO Nanoparticles Using Trichoderma spp. Isolated from Rhizosphere Soils and Its Synergistic Antibacterial Effect against Xanthomonas oryzae pv. oryzae." Journal of Fungi 6, no. 3: 181.

Journal article
Published: 03 September 2020 in Antibiotics
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Recently, titanium dioxide (TiO2) nanomaterials have gained increased attention because of their cost-effective, safe, stable, non-toxic, non-carcinogenic, photocatalytic, bactericidal, biomedical, industrial and waste-water treatment applications. The aim of the present work is the synthesis of electrospun TiO2 nanofibers (NFs) in the presence of different amounts of air–argon mixtures using sol-gel and electrospinning approaches. The physicochemical properties of the synthesized NFs were examined by scanning and transmission electron microscopies (SEM and TEM) coupled with energy-dispersive X-ray spectroscopy (EDX), ultraviolet-visible spectroscopy and thermogravimetric analyzer (TGA). The antibacterial and antibiofilm activity of synthesized NFs against Gram-negative Pseudomonas aeruginosa and Gram-positive methicillin-resistant Staphylococcusaureus (MRSA) was investigated by determining their minimum bacteriostatic and bactericidal values. The topological and morphological alteration caused by TiO2 NFs in bacterial cells was further analyzed by SEM. TiO2 NFs that were calcined in a 25% air-75% argon mixture showed maximum antibacterial and antibiofilm activities. The minimum inhibitory concentration (MIC)/minimum bactericidal concentration (MBC) value of TiO2 NFs against P. aeruginosa was 3 and 6 mg/mL and that for MRSA was 6 and 12 mg/mL, respectively. The MIC/MBC and SEM results show that TiO2 NFs were more active against Gram-negative P. aeruginosa cells than Gram-positive S. aureus. The inhibition of biofilm formation by TiO2 NFs was investigated quantitatively by tissue culture plate method using crystal violet assay and it was found that TiO2 NFs inhibited biofilm formation by MRSA and P. aeruginosa in a dose-dependent manner. TiO2 NFs calcined in a 25% air-75% argon mixture exhibited maximum biofilm formation inhibition of 75.2% for MRSA and 72.3% for P. aeruginosa at 2 mg/mL, respectively. The antibacterial and antibiofilm results suggest that TiO2 NFs can be used to coat various inanimate objects, in food packaging and in waste-water treatment and purification to prevent bacterial growth and biofilm formation.

ACS Style

Mohammad Azam Ansari; Hani Manssor Albetran; Muidh Hamed Alheshibri; Abdelmajid Timoumi; Norah Abdullah Algarou; Sultan Akhtar; Yassine Slimani; Munirah Abdullah Almessiere; Fatimah Saad AlAhmari; Abdulhadi Baykal; It-Meng Low. Synthesis of Electrospun TiO2 Nanofibers and Characterization of Their Antibacterial and Antibiofilm Potential against Gram-Positive and Gram-Negative Bacteria. Antibiotics 2020, 9, 572 .

AMA Style

Mohammad Azam Ansari, Hani Manssor Albetran, Muidh Hamed Alheshibri, Abdelmajid Timoumi, Norah Abdullah Algarou, Sultan Akhtar, Yassine Slimani, Munirah Abdullah Almessiere, Fatimah Saad AlAhmari, Abdulhadi Baykal, It-Meng Low. Synthesis of Electrospun TiO2 Nanofibers and Characterization of Their Antibacterial and Antibiofilm Potential against Gram-Positive and Gram-Negative Bacteria. Antibiotics. 2020; 9 (9):572.

Chicago/Turabian Style

Mohammad Azam Ansari; Hani Manssor Albetran; Muidh Hamed Alheshibri; Abdelmajid Timoumi; Norah Abdullah Algarou; Sultan Akhtar; Yassine Slimani; Munirah Abdullah Almessiere; Fatimah Saad AlAhmari; Abdulhadi Baykal; It-Meng Low. 2020. "Synthesis of Electrospun TiO2 Nanofibers and Characterization of Their Antibacterial and Antibiofilm Potential against Gram-Positive and Gram-Negative Bacteria." Antibiotics 9, no. 9: 572.

Editorial
Published: 31 August 2020 in Expert Opinion on Drug Delivery
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Mohammad Azam Ansari; Ahmad Almatroudi; Mohammad A. Alzohairy; Sami AlYahya; Mohammad N. Alomary; Hanan A. Al-Dossary; Saad Alghamdi. Lipid-based nano delivery of Tat-peptide conjugated drug or vaccine–promising therapeutic strategy for SARS-CoV-2 treatment. Expert Opinion on Drug Delivery 2020, 17, 1671 -1674.

AMA Style

Mohammad Azam Ansari, Ahmad Almatroudi, Mohammad A. Alzohairy, Sami AlYahya, Mohammad N. Alomary, Hanan A. Al-Dossary, Saad Alghamdi. Lipid-based nano delivery of Tat-peptide conjugated drug or vaccine–promising therapeutic strategy for SARS-CoV-2 treatment. Expert Opinion on Drug Delivery. 2020; 17 (12):1671-1674.

Chicago/Turabian Style

Mohammad Azam Ansari; Ahmad Almatroudi; Mohammad A. Alzohairy; Sami AlYahya; Mohammad N. Alomary; Hanan A. Al-Dossary; Saad Alghamdi. 2020. "Lipid-based nano delivery of Tat-peptide conjugated drug or vaccine–promising therapeutic strategy for SARS-CoV-2 treatment." Expert Opinion on Drug Delivery 17, no. 12: 1671-1674.

Journal article
Published: 02 August 2020 in Pathogens
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This study analyzed the genotype, antibiotic resistance, and biofilm formation of Acinetobacter baumannii strains and assessed the correlation between biofilm formation, antibiotic resistance, and biofilm-related risk factors. A total of 207 non-replicate multi-drug-resistant A. baumannii strains were prospectively isolated. Phenotypic identification and antimicrobial susceptibility testing were carried out. Isolate biofilm formation ability was evaluated using the tissue culture plate (TCP), Congo red agar, and tube methods. Clonal relatedness between the strains was assessed by enterobacterial repetitive intergenic consensus-PCR genotyping. Of the 207 isolates, 52.5% originated from an intensive care unit setting, and pan resistance was observed against ceftazidime and cefepime, with elevated resistance (99–94%) to piperacillin/tazobactam, imipenem, levofloxacin, and ciprofloxacin. alongside high susceptibility to tigecycline (97.8%). The Tissue culture plate, Tube method, and Congo red agar methods revealed that 53.6%, 20.8%, and 2.7% of the strains were strong biofilm producers, respectively, while a significant correlation was observed between biofilm formation and device-originating respiratory isolates (p = 0.0009) and between biofilm formation in colonized vs. true infection isolates (p = 0.0001). No correlation was detected between antibiotic resistance and biofilm formation capacity, and the majority of isolates were clonally unrelated. These findings highlight the urgent need for implementing strict infection control measures in clinical settings.

ACS Style

Aisha M. Alamri; Afnan A. Alsultan; Mohammad A. Ansari; Amani M. AlNimr. Biofilm-Formation in Clonally Unrelated Multidrug-Resistant Acinetobacter baumannii Isolates. Pathogens 2020, 9, 630 .

AMA Style

Aisha M. Alamri, Afnan A. Alsultan, Mohammad A. Ansari, Amani M. AlNimr. Biofilm-Formation in Clonally Unrelated Multidrug-Resistant Acinetobacter baumannii Isolates. Pathogens. 2020; 9 (8):630.

Chicago/Turabian Style

Aisha M. Alamri; Afnan A. Alsultan; Mohammad A. Ansari; Amani M. AlNimr. 2020. "Biofilm-Formation in Clonally Unrelated Multidrug-Resistant Acinetobacter baumannii Isolates." Pathogens 9, no. 8: 630.

Journal article
Published: 30 June 2020 in Biomolecules
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In this work, we aimed to synthesize zinc oxide nanoparticles (ZnONPs) using an aqueous extract of Cassia auriculata leaves (CAE) at room temperature without the provision of additional surfactants or capping agents. The formation of as-obtained ZnONPs was analyzed by UV–visible (ultraviolet) absorption and emission spectroscopy, X-ray photoemission spectroscopy (XPS), X-ray diffraction analysis (XRD), energy dispersive X-ray diffraction (EDX), thermogravimetric analysis/differential thermal analysis (TGA-DTA), scanning electron microscopy (SEM), transmission electron microscopy (TEM), high-resolution transmission electron microscopy (HRTEM), and selected area electron diffraction (SAED). The XRD results reflect the wurtzite structure of as-prepared ZnONPs, which produced diffraction patterns showing hexagonal phases. The SEM images indicate that the morphology of as-prepared ZnONPs is composed of hexagonal nanostructures with an average diameter of 20 nm. The HR-TEM result shows that the inter-planar distance between two lattice fringes is 0.260 nm, which coincides with the distance between the adjacent (d-spacing) of the (002) lattice plane of ZnO. The fluorescence emission spectrum of ZnONPs dispersed in ethanol shows an emission maximum at 569 nm, revealing the semiconductor nature of ZnO. As-obtained ZnONPs enhanced the tumoricidal property of CAE in MCF-7 breast cancer cells without significant inhibition of normal human breast cells, MCF-12A. Furthermore, we have studied the antibacterial effects of ZnONPs, which showed direct cell surface contact, resulting in the disturbance of bacterial cell integrity.

ACS Style

Kollur Shiva Prasad; Shashanka K. Prasad; Mohammad Azam Ansari; Mohammad A. Alzohairy; Mohammad N. Alomary; Sami Alyahya; Chandrashekar Srinivasa; Mahadevamurthy Murali; Veena Malligere Ankegowda; Chandan Shivamallu. Tumoricidal and Bactericidal Properties of ZnONPs Synthesized Using Cassia auriculata Leaf Extract. Biomolecules 2020, 10, 982 .

AMA Style

Kollur Shiva Prasad, Shashanka K. Prasad, Mohammad Azam Ansari, Mohammad A. Alzohairy, Mohammad N. Alomary, Sami Alyahya, Chandrashekar Srinivasa, Mahadevamurthy Murali, Veena Malligere Ankegowda, Chandan Shivamallu. Tumoricidal and Bactericidal Properties of ZnONPs Synthesized Using Cassia auriculata Leaf Extract. Biomolecules. 2020; 10 (7):982.

Chicago/Turabian Style

Kollur Shiva Prasad; Shashanka K. Prasad; Mohammad Azam Ansari; Mohammad A. Alzohairy; Mohammad N. Alomary; Sami Alyahya; Chandrashekar Srinivasa; Mahadevamurthy Murali; Veena Malligere Ankegowda; Chandan Shivamallu. 2020. "Tumoricidal and Bactericidal Properties of ZnONPs Synthesized Using Cassia auriculata Leaf Extract." Biomolecules 10, no. 7: 982.

Journal article
Published: 16 June 2020 in Saudi Journal of Biological Sciences
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Present study, report the biofabrication of zinc oxide nanoparticles from aqueous leaf extract of Melia azedarach (MaZnO-NPs) through solution combustion method and their novel application in preventing the growth of seed-borne fungal pathogens of soybean (Cladosporium cladosporioides and Fusarium oxysporum). The standard blotter method was employed to isolate fungi and was identified through molecular techniques. The characterization of MaZnO-NPs was carried out by UV–Vis spectroscopy, Fourier Transform Infrared Spectroscopy (FT-IR), X-Ray Diffraction (XRD), Scanning Electron Microscopy (SEM) equipped with Energy Dispersive Spectroscopy (EDS) and Transmission Electron Microscopy (TEM). The physicochemical characterization confirmed the particles were of high purity and nano size (30–40 nm) with a hexagonal shape. The synthesized MaZnO-NPs inhibited the growth of C. cladosporioides and F. oxysporum in a dose dependent manner. Biomass, ergosterol, lipid peroxidation, intracellular reactive oxygen species and membrane integrity determination upon MaZnO-NPs treatment offered significant activities there by confirming the mechanism of action against the test pathogens. In conclusion, due to the effectiveness of MaZnO-NPs in controlling the growth of C. cladosporioides and F. oxysporum, the synthesized MaZnO-NPs provides insight towards their potential application in agriculture and food industries.

ACS Style

T.R. Lakshmeesha; M. Murali; Mohammad Azam Ansari; Arakere C. Udayashankar; Mohammad A. Alzohairy; Ahmad Almatroudi; Mohammad N. Alomary; Sarah Mousa Maadi Asiri; B.S. Ashwini; Naveen Kumar Kalagatur; Chandra S. Nayak; S.R. Niranjana. Biofabrication of zinc oxide nanoparticles from Melia azedarach and its potential in controlling soybean seed-borne phytopathogenic fungi. Saudi Journal of Biological Sciences 2020, 27, 1923 -1930.

AMA Style

T.R. Lakshmeesha, M. Murali, Mohammad Azam Ansari, Arakere C. Udayashankar, Mohammad A. Alzohairy, Ahmad Almatroudi, Mohammad N. Alomary, Sarah Mousa Maadi Asiri, B.S. Ashwini, Naveen Kumar Kalagatur, Chandra S. Nayak, S.R. Niranjana. Biofabrication of zinc oxide nanoparticles from Melia azedarach and its potential in controlling soybean seed-borne phytopathogenic fungi. Saudi Journal of Biological Sciences. 2020; 27 (8):1923-1930.

Chicago/Turabian Style

T.R. Lakshmeesha; M. Murali; Mohammad Azam Ansari; Arakere C. Udayashankar; Mohammad A. Alzohairy; Ahmad Almatroudi; Mohammad N. Alomary; Sarah Mousa Maadi Asiri; B.S. Ashwini; Naveen Kumar Kalagatur; Chandra S. Nayak; S.R. Niranjana. 2020. "Biofabrication of zinc oxide nanoparticles from Melia azedarach and its potential in controlling soybean seed-borne phytopathogenic fungi." Saudi Journal of Biological Sciences 27, no. 8: 1923-1930.