Precision livestock farming (PLF) technologies are becoming increasingly common in modern agriculture. They are frequently integrated with other new technologies in order to improve human-livestock interactions, productivity and economical sustainability of modern farms. New systems are constantly being developed for concentrated farming operations as well as for extensive and pasture-based farming systems. The development of technologies for grazing animals is of particular interest for the Mediterranean extensive sheep farming sector. Dairy sheep farming is a typical production system of the area linked to its historical and cultural traditions. The area provides roughly 40% of the world sheep milk, having 27% of the milk-producing ewes. Developed countries of the area (France, Italy, Greece and Spain - FIGS) have highly specialized production systems improved through animal selection, feeding techniques and intensification of production. However, extensive systems are still practiced alongside intensive ones due to their lower input costs and better resilience to market fluctuations. In the current article, we evaluate possible PLF systems and their suitability to be incorporated in extensive dairy sheep farming as practiced in the FIGS countries. Available products include: electronic identification systems (now mandatory in the EU) such as ear tags, ruminal boluses and sub-cutaneous radio-frequency identification; on-animal sensors such as accelerometers, global positioning systems and social activity loggers; and stationary management systems such as walk-over-weights, automatic drafter (AD), virtual fencing and milking parlour-related technologies. The systems were considered according to their suitability for the management and business model common in dairy sheep farming. However, adoption of new technologies does not take place immediately in small and medium scale extensive farming. As sheep farmers usually belong to more conservative technology consumers, characterized by an average age of 60 and a very transparent community, the dynamics do not favour financial risk taking involved with new technologies. Financial barriers linked to production volumes and resource management of extensive farming are also a barrier for innovation. However, future prospectives could increase the importance of technology and promote its wider adoption. Trends such as global sheep milk economics, global warming, awareness to animal welfare, antibiotics resistance and European agricultural policies could influence the farming practices and stimulate wider adoption of PLF systems in the near future.

The agro-food industry produces tons of waste at different stages in the food production process, creating a massive ecological crisis. If implemented, the use of fruit and vegetable by-products (FVBPs) in animal nutrition has the potential to lessen the environmental footprint of the food production chain, lower animal feeding costs, and improve the quality and sustainability of animal products. Recent research on the inclusion of FVBPs, naturally enriched with polyphenols, in the diets of small and large ruminants has shown some promising outcomes, which we discuss in this review. The effects of FVBPs on digestion, rumen fermentation, methane emissions, rumen liquor fatty acid profile, and milk production are examined. Due to the chemical composition and the presence of certain bioactive compounds, FVBPs are capable of influencing the ruminal and intestinal ecosystem through improved kinetics of fermentation. Several in vivo studies have demonstrated that the dietary inclusion of FVBPs resulted in improved milk production and composition without any negative effect on animal performance. Using FVBPs as an alternative to conventional feedstuffs may promote sustainable animal production and nutrition. However, it must be stressed that the efficacy of these feed supplements is conditional on the source, kind, and quantity employed.

The adult mammalian brain is mainly composed of mature neurons. A limited amount of stem cell-driven neurogenesis persists in postnatal life and is reduced in large-brained species. Another source of immature neurons in adult brains is cortical layer II. These cortical immature neurons (cINs) retain developmentally undifferentiated states in adulthood, though they are generated before birth. Here, the occurrence, distribution and cellular features of cINs were systematically studied in 12 diverse mammalian species spanning from small-lissencephalic to large-gyrencephalic brains. In spite of well-preserved morphological and molecular features, the distribution of cINs was highly heterogeneous, particularly in neocortex. While virtually absent in rodents, they are present in the entire neocortex of many other species and their linear density in cortical layer II generally increased with brain size. These findings suggest an evolutionary developmental mechanism for plasticity that varies among mammalian species, granting a reservoir of young cells for the cerebral cortex.

No data have been published on the use of infrared thermography (IRT) to evaluate sheep emotions. We assessed whether this technique can be used as a non-invasive measure of negative emotions. Two voluntary animal approach (VAA) tests were conducted (and filmed) on five ewes before and after being restrained. The restraining process was performed by a handler for five minutes. IRT was used during restraint and the VAA tests. The lacrimal caruncle temperature was significantly higher during restraint and in the VAA test after the restraint compared with the VAA test before the restraint (Wilcoxon’s test; p = 0.04). The latency period until first contact was longer in the second VAA test (132 s) than in the first one (60 s). Our preliminary results suggest that IRT, combined with behavioral data, is a non-invasive technique that can be useful to assess stress and infer about negative emotions in sheep.

• The heart rate sensors measured reliable heart rates and assigned the quality correctly • Free grazing sheep had passive heart rates of 90 bpm to 112 bpm depending on age • Body temperature followed a convex curve peaking in summer • Heart rate followed a concave curve peaking in summer • All sheep's body temperature displayed 24-hour circadian rhythms

The transition to a more sustainable livestock sector represents one of the major challenges of our time. Life Cycle Assessment (LCA) is recognised as the gold standard methodology for assessing the environmental impact of farming systems. Simultaneously, animal welfare is a key component of livestock production and is intrinsically related to human and environmental well-being. To perform an overall on-farm sustainability assessment, it would be desirable to consider both the environmental impact and the welfare of the animals. The present work aimed to summarise and describe the methodologies adopted in peer-reviewed papers published to date, that combine animal welfare evaluation with LCA. Citations, retrieved from four bibliographical databases, were systematically evaluated in a multi-stage approach following the JBI and PRISMA scoping review guidelines. The searches identified 1 460 studies, of which only 24 were compliant with the inclusion criteria. The results highlighted how the environmental LCA was undertaken with a much more homogenous and standardised method than animal welfare assessment. When studies were grouped based on the type of animal welfare assessment performed: 16.7% used single welfare indicators, 45.8% multiple indicators, 8.3% applied existing validated protocols (i.e., TGI-200 and TGI-35L), 16.7% used non-validated protocols and 12.5% employed other methods. The papers were further classified with respect to the "5 Animal Welfare Domains Model": the most assessed domain was "environment" (90.5% of the papers%), followed by "health" (52.4%), "nutrition" (33.3%), "behavioural interactions" (28.6%) and "mental state" (9.5%). None of the studies assessed all the domains simultaneously. In addition, 66.7% of papers (n = 16) aggregated the animal welfare indicators into a final score. Within these, only four papers proposed to associate the animal welfare scores with the LCA functional unit. An overall sustainability score, calculated with several different approaches to summarise the information, was provided by 46% of the papers. In summary, despite the topic's relevance, to date, there is neither a consensus on the animal welfare assessment approach to be carried out (indicators selection and their aggregation) nor on the standardisation of an integrated animal welfare-LCA evaluation. The present review provides a basis for the development of common future guidelines to carry out a comprehensive, true-to-life and robust farm sustainability assessment.