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For Scientific contribution under the age of 40
International Scientist Awards on Engineering, Science and Medicine 2021
For academic and scientific contribution in Mexico
Embassy of India, Mexico
For scientific contribution in COVID19 research
Tecnologico de Monterrey
Dr. Ashutosh Sharma currently works as the Director of the Regional Bioengineering Department, South-Central Region at Tecnologico de Monterrey (QS world ranking Top# 30 private university) at Queretaro city campus, Mexico. He served as Director of Biotechnology Engineering Program at the Querétaro campus during the years 2013-2017. In 2017 Dr. Sharma obtained the Full Profesor Title, being the youngest full professor of the campus. He is a fellow of the prestigious Mexican National System of Researchers since 2014. He has authored more than 75 scientific contributions, including JCR indexed articles, patents, book chapters, books and conference papers. Dr. Sharma has also developed commercially successful technologies and products in the agrobiotechnology and phytomedicine field. He is the founder-director and promoter of the Bioengineering Research Center, at Queretaro campus. Where he has established a state-of-art OMICS laboratory and consolidated a highly productive research group. He serves as an invited reviewer for more than 24 JCR journals, including publishers such as Springer, Elsevier, BMC, Frontiers, Nature, Dove Medical Press, Bentham etc. Dr. Sharma has participated in dozens of communication forums, including newspapers, radio and television on diverse scientific topics. He has more than 30 research papers presented in countries like the USA, Holland, Brazil, Mexico, Canada, China, Chile, Spain, and India.
Cannabinoids are the major chemical constituents of the plant Cannabis sativa L. and are known to exhibit a wide range of pharmacological effects viz., psychotropic, analgesic, anticancer, antiinflammatory, antidiabetic, anticonvulsive, antibacterial and antifungal etc. The use of cannabis, cannabinoids and their products is restricted in several countries due to the high risk of misuse. Recently, cannabinoids have regained the interest of the researchers due to their therapeutic applications. Ever since the discovery of the cannabinoids, most of the studies carried out on the evaluation of their biological activities were limited to only preclinical levels. The quality of the preclinical data still remains only low to moderate, thus, leaving behind an uncertainty in their use for therapeutic applications. Problems associated with the solubility, stability and bioavailability of the cannabinoid drugs are also a major concern in the quality of the study. Nanoparticle based drug delivery system could be a potential method to increase the reliability of the data. While considering the immense pharmacological properties of the cannabinoids, there is an urgency to perform intensive clinical trials and to know their mechanism of action in various disease conditions, evaluate their efficacy and safety, and register them as drug candidates. This review highlights the chemistry, types and biological activities of the cannabinoids such as THC, CBD and CBN in focus with their anticancer activity, neuroprotective effect and nanoformulating the cannabinoid drugs.
Arivalagan Pugazhendhi; Natarajan Suganthy; Tan Phat Chau; Ashutosh Sharma; Yuwalee Unpaprom; Rameshprabu Ramaraj; Indira Karuppusamy; Kathirvel Brindhadevi. Cannabinoids as anticancer and neuroprotective drugs: Structural insights and pharmacological interactions—A review. Process Biochemistry 2021, 1 .
AMA StyleArivalagan Pugazhendhi, Natarajan Suganthy, Tan Phat Chau, Ashutosh Sharma, Yuwalee Unpaprom, Rameshprabu Ramaraj, Indira Karuppusamy, Kathirvel Brindhadevi. Cannabinoids as anticancer and neuroprotective drugs: Structural insights and pharmacological interactions—A review. Process Biochemistry. 2021; ():1.
Chicago/Turabian StyleArivalagan Pugazhendhi; Natarajan Suganthy; Tan Phat Chau; Ashutosh Sharma; Yuwalee Unpaprom; Rameshprabu Ramaraj; Indira Karuppusamy; Kathirvel Brindhadevi. 2021. "Cannabinoids as anticancer and neuroprotective drugs: Structural insights and pharmacological interactions—A review." Process Biochemistry , no. : 1.
MicroRNA-mediated gene regulation in non-vascular plants is potentially involved in several unique biological functions, including biosynthesis of several highly valuable exclusive bioactive compounds, and those small RNAs could be manipulated for the overproduction of essential bioactive compounds in the future. MicroRNAs (miRNAs) are a class of endogenous, small (20–24 nucleotides), non-coding RNA molecules that regulate gene expression through the miRNA-mediated mechanisms of either translational inhibition or messenger RNA (mRNA) cleavage. In the past years, studies have mainly focused on elucidating the roles of miRNAs in vascular plants as compared to non-vascular plants. However, non-vascular plant miRNAs have been predicted to be involved in a wide variety of specific biological mechanisms; nevertheless, some of them have been demonstrated explicitly, thus showing that the research field of this plant group owns a noteworthy potential to develop novel investigations oriented towards the functional characterization of these miRNAs. Furthermore, the insights into the roles of miRNAs in non-vascular plants might be of great importance for designing the miRNA-based genetically modified plants for valuable secondary metabolites, active compounds, and biofuels in the future. Therefore, in this current review, we provide an overview of the potential roles of miRNAs in different groups of non-vascular plants such as algae and bryophytes.
Sujay Paul; Luis Alberto Bravo Vázquez; Marilyn Márquez Nafarrate; Ana Isabel Gutiérrez Reséndiz; Aashish Srivastava; Ashutosh Sharma. The regulatory activities of microRNAs in non-vascular plants: a mini review. Planta 2021, 254, 1 -23.
AMA StyleSujay Paul, Luis Alberto Bravo Vázquez, Marilyn Márquez Nafarrate, Ana Isabel Gutiérrez Reséndiz, Aashish Srivastava, Ashutosh Sharma. The regulatory activities of microRNAs in non-vascular plants: a mini review. Planta. 2021; 254 (3):1-23.
Chicago/Turabian StyleSujay Paul; Luis Alberto Bravo Vázquez; Marilyn Márquez Nafarrate; Ana Isabel Gutiérrez Reséndiz; Aashish Srivastava; Ashutosh Sharma. 2021. "The regulatory activities of microRNAs in non-vascular plants: a mini review." Planta 254, no. 3: 1-23.
Plants are a remarkable source of high-value specialized metabolites having significant physiological and ecological functions. Genes responsible for synthesizing specialized metabolites are often clustered together for a coordinated expression, which is commonly observed in bacteria and filamentous fungi. Similar to prokaryotic gene clustering, plants do have gene clusters encoding enzymes involved in the biosynthesis of specialized metabolites. More than 20 gene clusters involved in the biosynthesis of diverse metabolites have been identified across the plant kingdom. Recent studies demonstrate that gene clusters are evolved through gene duplications and neofunctionalization of primary metabolic pathway genes. Often, these clusters are tightly regulated at nucleosome level. The prevalence of gene clusters related to specialized metabolites offers an attractive possibility of an untapped source of highly useful biomolecules. Accordingly, the identification and functional characterization of novel biosynthetic pathways in plants need to be worked out. In this review, we summarize insights into the evolution of gene clusters and discuss the organization and importance of specific gene clusters in the biosynthesis of specialized metabolites. Regulatory mechanisms which operate in some of the important gene clusters have also been briefly described. Finally, we highlight the importance of gene clusters to develop future metabolic engineering or synthetic biology strategies for the heterologous production of novel metabolites.
Revuru Bharadwaj; Sarma R. Kumar; Ashutosh Sharma; Ramalingam Sathishkumar. Plant Metabolic Gene Clusters: Evolution, Organization, and Their Applications in Synthetic Biology. Frontiers in Plant Science 2021, 12, 1 .
AMA StyleRevuru Bharadwaj, Sarma R. Kumar, Ashutosh Sharma, Ramalingam Sathishkumar. Plant Metabolic Gene Clusters: Evolution, Organization, and Their Applications in Synthetic Biology. Frontiers in Plant Science. 2021; 12 ():1.
Chicago/Turabian StyleRevuru Bharadwaj; Sarma R. Kumar; Ashutosh Sharma; Ramalingam Sathishkumar. 2021. "Plant Metabolic Gene Clusters: Evolution, Organization, and Their Applications in Synthetic Biology." Frontiers in Plant Science 12, no. : 1.
MicroRNAs (miRNAs) are small (∼21 nucleotides), endogenous, non-coding RNA molecules implicated in the post-transcriptional gene regulation performed through target mRNA cleavage or translational inhibition. In recent years, several investigations have demonstrated that miRNAs are involved in regulating both carbohydrate and lipid homeostasis in humans and other organisms. Moreover, it has been observed that the dysregulation of these metabolism-related miRNAs leads to the development of several metabolic disorders, such as type 2 diabetes, obesity, nonalcoholic fatty liver, insulin resistance, and hyperlipidemia. Hence, in this current review, with the aim to impulse the research arena of the micro-transcriptome implications in vital metabolic pathways as well as to highlight the remarkable potential of miRNAs as therapeutic targets for metabolic disorders in humans, we provide an overview of the regulatory roles of metabolism-associated miRNAs in humans and murine models.
Sujay Paul; Luis Alberto Bravo Vázquez; Samantha Pérez Uribe; Luis Aarón Manzanero Cárdenas; María Fernanda Ruíz Aguilar; Samik Chakraborty; Ashutosh Sharma. Roles of microRNAs in carbohydrate and lipid metabolism disorders and their therapeutic potential. Biochimie 2021, 187, 83 -93.
AMA StyleSujay Paul, Luis Alberto Bravo Vázquez, Samantha Pérez Uribe, Luis Aarón Manzanero Cárdenas, María Fernanda Ruíz Aguilar, Samik Chakraborty, Ashutosh Sharma. Roles of microRNAs in carbohydrate and lipid metabolism disorders and their therapeutic potential. Biochimie. 2021; 187 ():83-93.
Chicago/Turabian StyleSujay Paul; Luis Alberto Bravo Vázquez; Samantha Pérez Uribe; Luis Aarón Manzanero Cárdenas; María Fernanda Ruíz Aguilar; Samik Chakraborty; Ashutosh Sharma. 2021. "Roles of microRNAs in carbohydrate and lipid metabolism disorders and their therapeutic potential." Biochimie 187, no. : 83-93.
Neem (Azadirachta indica) is a very popular traditional medicinal plant used since ancient times to treat numerous ailments. MicroRNAs (miRNAs) are highly conserved, non-coding, short RNA molecules that play important regulatory roles in plant development and metabolism. In this study, deploying a high stringent genome-wide computational-based approach and following a set of strict filtering norms a total of 44 potential conserved neem miRNAs belonging to 21 families and their corresponding 48 potential target transcripts were identified. Important targets include Squamosa promoter binding protein-like proteins, NAC, Scarecrow proteins, Auxin response factor, and F-box proteins. A biological network has also been developed to understand the miRNA-mediated gene regulation using the minimum free energy (MFE) values of the miRNA-target interaction. Moreover, six selected miRNAs were reported to be involved in secondary metabolism in other plant species (miR156a, miR156l, miR160, miR164, miR171, miR395) were validated by qPCR and their tissue-specific differential expression pattern was observed in leaves and stem. Except for ain-miR395, all the other miRNAs were found overexpressed in the stem as compared to leaves. To the best of our knowledge, this is the first report of neem miRNAs and we believe the finding of the present study will be useful for the functional genomic study of medicinal plants.
Sujay Paul; Paula Reyes-Pérez; Paola Isabel Angulo-Bejarano; Aashish Srivastava; Sathishkumar Ramalingam; Ashutosh Sharma. Characterization of microRNAs from neem (Azadirachta indica) and their tissue-specific expression study in leaves and stem. 3 Biotech 2021, 11, 1 -12.
AMA StyleSujay Paul, Paula Reyes-Pérez, Paola Isabel Angulo-Bejarano, Aashish Srivastava, Sathishkumar Ramalingam, Ashutosh Sharma. Characterization of microRNAs from neem (Azadirachta indica) and their tissue-specific expression study in leaves and stem. 3 Biotech. 2021; 11 (6):1-12.
Chicago/Turabian StyleSujay Paul; Paula Reyes-Pérez; Paola Isabel Angulo-Bejarano; Aashish Srivastava; Sathishkumar Ramalingam; Ashutosh Sharma. 2021. "Characterization of microRNAs from neem (Azadirachta indica) and their tissue-specific expression study in leaves and stem." 3 Biotech 11, no. 6: 1-12.
Potable and good-quality drinking water availability is a serious global concern, since several pollution sources significantly contribute to low water quality. Amongst these pollution sources, several are releasing an array of hazardous agents into various environmental and water matrices. Unfortunately, there are not very many ecologically friendly systems available to treat the contaminated environment exclusively. Consequently, heavy metal water contamination leads to many diseases in humans, such as cardiopulmonary diseases and cytotoxicity, among others. To solve this problem, there are a plethora of emerging technologies that play an important role in defining treatment strategies. Phytoremediation, the usage of plants to remove contaminants, is a technology that has been widely used to remediate pollution in soils, with particular reference to toxic elements. Thus, hydroponic systems coupled with bioremediation for the removal of water contaminants have shown great relevance. In this review, we addressed several studies that support the development of phytoremediation systems in water. We cover the importance of applied science and environmental engineering to generate sustainable strategies to improve water quality. In this context, the phytoremediation capabilities of different plant species and possible obstacles that phytoremediation systems may encounter are discussed with suitable examples by comparing different mechanistic processes. According to the presented data, there are a wide range of plant species with water phytoremediation potential that need to be studied from a multidisciplinary perspective to make water phytoremediation a viable method.
Cristián Delgado-González; Alfredo Madariaga-Navarrete; José Fernández-Cortés; Margarita Islas-Pelcastre; Goldie Oza; Hafiz Iqbal; Ashutosh Sharma. Advances and Applications of Water Phytoremediation: A Potential Biotechnological Approach for the Treatment of Heavy Metals from Contaminated Water. International Journal of Environmental Research and Public Health 2021, 18, 5215 .
AMA StyleCristián Delgado-González, Alfredo Madariaga-Navarrete, José Fernández-Cortés, Margarita Islas-Pelcastre, Goldie Oza, Hafiz Iqbal, Ashutosh Sharma. Advances and Applications of Water Phytoremediation: A Potential Biotechnological Approach for the Treatment of Heavy Metals from Contaminated Water. International Journal of Environmental Research and Public Health. 2021; 18 (10):5215.
Chicago/Turabian StyleCristián Delgado-González; Alfredo Madariaga-Navarrete; José Fernández-Cortés; Margarita Islas-Pelcastre; Goldie Oza; Hafiz Iqbal; Ashutosh Sharma. 2021. "Advances and Applications of Water Phytoremediation: A Potential Biotechnological Approach for the Treatment of Heavy Metals from Contaminated Water." International Journal of Environmental Research and Public Health 18, no. 10: 5215.
Background: The world's population is still growing, having an impact on the environment and the economic growth of developing countries; so that, there is a particular interest in the development of new fertility control methods, focused on male contraception. Objective: The objective of this study was to evaluate the effect of methanolic extracts of leaf and fruit of Azadirachta indica on sperm quality and testicular histology of Long Evans rats. Methods: Antifertility effects of a methanolic leaf and fruit extracts of A. indica on 24 male rats were investigated. The animals were randomly divided into two control groups and four treatment groups (n=4). Doses of the leaf and fruit extract were given at concentrations of 100 and 200 μg mL-1. Results: A significant decrease in the viability of sperm cells was observed. The leaf extract at a concentration of 200 μg mL-1 inhibited cell viability compared to the negative control (p< 0.001). The percentage of abnormal cells in leaf extract was shown in 100 and 200 μg mL-1, the conditions at which a higher percentage of morphological irregularities of observed (15% and 16% respectively). The results show that there was cellular detachment in the seminiferous epithelium in the experimental groups treated with methanolic extracts. Sperm death was observed without decreasing the number of sperm. Conclusion: The methanolic extracts of Azadirachta indica have a modulating effect on the spermatogenesis of experimental rats through sperm morphological alterations.
Castillo-Maldonado Irais; Borjón-Ríos Claudia; Pedroza-Escobar David; Sharma Ashutosh; García-Garza Rubén; Ramírez-Moreno Agustina; Vega-Menchaca M. del Carmen; Rivera-Guillén Mario-Alberto; Serrano-Gallardo Luis-Benjamín. Leaf and Fruit Methanolic Extracts of Azadirachta indica Exhibit Antifertility Activity on Rats’ Sperm Quality and Testicular Histology. Current Pharmaceutical Biotechnology 2021, 22, 400 -407.
AMA StyleCastillo-Maldonado Irais, Borjón-Ríos Claudia, Pedroza-Escobar David, Sharma Ashutosh, García-Garza Rubén, Ramírez-Moreno Agustina, Vega-Menchaca M. del Carmen, Rivera-Guillén Mario-Alberto, Serrano-Gallardo Luis-Benjamín. Leaf and Fruit Methanolic Extracts of Azadirachta indica Exhibit Antifertility Activity on Rats’ Sperm Quality and Testicular Histology. Current Pharmaceutical Biotechnology. 2021; 22 (3):400-407.
Chicago/Turabian StyleCastillo-Maldonado Irais; Borjón-Ríos Claudia; Pedroza-Escobar David; Sharma Ashutosh; García-Garza Rubén; Ramírez-Moreno Agustina; Vega-Menchaca M. del Carmen; Rivera-Guillén Mario-Alberto; Serrano-Gallardo Luis-Benjamín. 2021. "Leaf and Fruit Methanolic Extracts of Azadirachta indica Exhibit Antifertility Activity on Rats’ Sperm Quality and Testicular Histology." Current Pharmaceutical Biotechnology 22, no. 3: 400-407.
Parkinson’s disease (PD) is a progressive neurodegenerative disease with no definite molecular markers for diagnosis. Metal exposure may alter cellular proteins that contribute to PD. Exploring the cross-talk between metal and its binding proteins in PD could reveal a new strategy for PD diagnosis. We performed a meta-analysis from different PD tissue microarray datasets to identify differentially expressed genes (DEGs) common to the blood and brain. Among common DEGs, we extracted 280 metalloprotein-encoding genes to construct protein networks describing the regulation of metalloproteins in the PD blood and brain. From the metalloprotein network, we identified three important functional hubs. Further analysis shows 60S ribosomal protein L6 (RPL6), a novel intermediary molecule connecting the three hubs of the metalloproteins network. Quantitative real-time PCR analysis showed that RPL6 was downregulated in PD peripheral blood mononuclear cell (PBMC) samples. Simultaneously, trace element analysis revealed altered serum zinc and magnesium concentrations in PD samples. The Pearson’s correlation analysis shows that serum zinc and magnesium regulate the RPL6 gene expression in PBMC. Thus, metal-regulating RPL6 acts as an intermediary molecule connecting the three hubs that are functionally associated with PD. Overall our study explores the understanding of metal-mediated pathogenesis in PD, which provides a serum metal environment regulating the cellular gene expression that may light toward metal and gene expression-based biomarkers for PD diagnosis.
Athira Anirudhan; Paola Isabel Angulo-Bejarano; Prabu Paramasivam; Kalaivani Manokaran; S. Manjunath Kamath; Ram Murugesan; Ashutosh Sharma; Shiek S. S. J. Ahmed. RPL6: A Key Molecule Regulating Zinc- and Magnesium-Bound Metalloproteins of Parkinson’s Disease. Frontiers in Neuroscience 2021, 15, 1 .
AMA StyleAthira Anirudhan, Paola Isabel Angulo-Bejarano, Prabu Paramasivam, Kalaivani Manokaran, S. Manjunath Kamath, Ram Murugesan, Ashutosh Sharma, Shiek S. S. J. Ahmed. RPL6: A Key Molecule Regulating Zinc- and Magnesium-Bound Metalloproteins of Parkinson’s Disease. Frontiers in Neuroscience. 2021; 15 ():1.
Chicago/Turabian StyleAthira Anirudhan; Paola Isabel Angulo-Bejarano; Prabu Paramasivam; Kalaivani Manokaran; S. Manjunath Kamath; Ram Murugesan; Ashutosh Sharma; Shiek S. S. J. Ahmed. 2021. "RPL6: A Key Molecule Regulating Zinc- and Magnesium-Bound Metalloproteins of Parkinson’s Disease." Frontiers in Neuroscience 15, no. : 1.
Micronutrient deficiency results in malnutrition, which is prevalent all over the world and may lead to premature death in women and children (White and Broadley, 2009). Strategies formulated earlier including supplementation and fortified foods were not successful, owing to socio-economic and technical hurdles (Mayer et al. 2008). The subsequently evolved strategy of bio-fortification is a viable biotechnological tool to achieve desired results without compromising the agronomical values of crops. Conferring the genetic trait to improve vital nutrient accumulation in the edible parts of staple food crops, such as rice, through metabolic engineering is considered a fast, sustainable, and cost-effective alternative to conventional breeding (Maestre et al. 2017). In earlier reports from our lab, enhanced α-tocopherol levels in the stable transformants of Nicotiana tabacum (Harish et al. 2013a, b) and in Nicotiana benthamiana adopting a transient expression system using A. thaliana tocopherol cyclase (TC) and homogentisate phytyl transferase (HPT) (Sathish et al. 2018) were shown. In the present study, Agrobacterium-mediated transformation of Indica rice ASD16 with two genes involved in tocopherol biosynthesis, viz., TC and HPT were carried out and the transgenic plants were analyzed for the vitamin E (α- tocopherol) content.
Sathish Sundararajan; Venkatesh Rajendran; Hari Priya Sivakumar; Safia Nayeem; Harish Mani Chandra; Ashutosh Sharma; Sathishkumar Ramalingam. Enhanced vitamin E content in an Indica rice cultivar harbouring two transgenes from Arabidopsis thaliana involved in tocopherol biosynthesis pathway. Plant Biotechnology Journal 2021, 19, 1083 -1085.
AMA StyleSathish Sundararajan, Venkatesh Rajendran, Hari Priya Sivakumar, Safia Nayeem, Harish Mani Chandra, Ashutosh Sharma, Sathishkumar Ramalingam. Enhanced vitamin E content in an Indica rice cultivar harbouring two transgenes from Arabidopsis thaliana involved in tocopherol biosynthesis pathway. Plant Biotechnology Journal. 2021; 19 (6):1083-1085.
Chicago/Turabian StyleSathish Sundararajan; Venkatesh Rajendran; Hari Priya Sivakumar; Safia Nayeem; Harish Mani Chandra; Ashutosh Sharma; Sathishkumar Ramalingam. 2021. "Enhanced vitamin E content in an Indica rice cultivar harbouring two transgenes from Arabidopsis thaliana involved in tocopherol biosynthesis pathway." Plant Biotechnology Journal 19, no. 6: 1083-1085.
Chikungunya (CHIKV) and Dengue (DENV) viruses cause an acute febrile illness which is hard to clinically differentiate and treat since both exhibit similar symptoms. Hence, this study was aimed at identifying the expression profiles of cytokines on co-infected samples and compare with CHIKV and DENV mono-infected samples. Serum samples of 292 suspected patients during 2009-2011 were analyzed. The presence of primary (IgM)/secondary (IgG) antibodies and early NS1 Dengue antigens were confirmed by capture ELISA. Molecular diagnosis and serotypes were discriminated by RT-PCR, confirmed by sequencing. All the plasma samples were assayed for cytokine expression by BDTM cytometry bead array (CBA) and compared with independent mono-infection viral load. Among the tested samples, 82 were confirmed as Dengue positive; 52 through IgM (17.8%), and 30 through IgG (10.2%). Additionally, 186 samples were confirmed as Chikungunya, 96 through IgM (32.6%) and 92 through IgG (31.5%) ELISA, respectively. Interestingly, 19 patients were co-infection positive in which, only 6 were confirmed for CHIKV and 7 for DENV by RT-PCR. Among 8 cytokines, IL-2, IL-8, IFNα, IFN γ, and IL-12 were found to be significantly different between co-infected and CHIKV mono-infected patients and correlated with viral load. DENV viral load was correlated with cytokine expression and a significant difference in IL-2 and IL-12 was observed between DENV mono-infected and DENV and CHIKV co-infected patients. Results indicated that apart from serological and molecular confirmation, cytokines could be used as a specific biomarker for the diagnosis of DENV and CHIKV. In the future, the role of independent cytokines can be determined to understand the pathogenesis and etiology of these dreadful diseases.
Saravana Krishnan; Jayashri Mahalingam; Shanthi Sabarimurugan; Thiruvengadam Muthu; Baskar Venkidasamy; Kaveri Krishnasamy; Ashutosh Sharma; Sathishkumar Ramalingam. Comparison of Cytokine Expression Profile in Chikungunya and Dengue Co-Infected and Mono-Infected Patients’ Samples. Pathogens 2021, 10, 166 .
AMA StyleSaravana Krishnan, Jayashri Mahalingam, Shanthi Sabarimurugan, Thiruvengadam Muthu, Baskar Venkidasamy, Kaveri Krishnasamy, Ashutosh Sharma, Sathishkumar Ramalingam. Comparison of Cytokine Expression Profile in Chikungunya and Dengue Co-Infected and Mono-Infected Patients’ Samples. Pathogens. 2021; 10 (2):166.
Chicago/Turabian StyleSaravana Krishnan; Jayashri Mahalingam; Shanthi Sabarimurugan; Thiruvengadam Muthu; Baskar Venkidasamy; Kaveri Krishnasamy; Ashutosh Sharma; Sathishkumar Ramalingam. 2021. "Comparison of Cytokine Expression Profile in Chikungunya and Dengue Co-Infected and Mono-Infected Patients’ Samples." Pathogens 10, no. 2: 166.
Isoflavonoids, the diverse group of secondary metabolites derived from the phenylpropanoid pathway, are distributed predominantly in leguminous plants and play a vital role in promoting human health. Genetic engineering of the metabolite synthesis pathway has turned out to be an attractive approach for the production of various secondary metabolites. In our study, we attempted to produce the isoflavone genistein, a well-known health-promoting metabolite, in Allium cepa L. (onion) by introducing Glycine max Isoflavone synthase (GmIFS). The GmIFS gene was cloned into the pEarleyGate 102 HA vector and transformed into onion by Agrobacterium-mediated and biolistic methods. The presence of GmIFS in transgenic onion was confirmed by PCR, dot blot, and Southern hybridization. Analysis of the transgenic onion calli lines demonstrated that the expression of the GmIFS gene led to the production of isoflavone genistein in in vitro tissues. The biolistic stable transformed calli with transformation efficiency of 73% (62.65 nM/g FW) accumulated more genistein than the Agrobacterium stable transformed calli with transformation efficiency of 56% (42.5 nM/g FW). Overall, heterologous gene expression of GmIFS was demonstrated by modifying the secondary metabolite pathway in onion tissues for the production of isoflavone genistein that can boost up human health with its health-promoting properties.
Ashwini Malla; Balamurugan Shanmugaraj; Balamurugan Srinivasan; Ashutosh Sharma; Sathishkumar Ramalingam. Metabolic Engineering of Isoflavonoid Biosynthesis by Expressing Glycine max Isoflavone Synthase in Allium cepa L. for Genistein Production. Plants 2020, 10, 52 .
AMA StyleAshwini Malla, Balamurugan Shanmugaraj, Balamurugan Srinivasan, Ashutosh Sharma, Sathishkumar Ramalingam. Metabolic Engineering of Isoflavonoid Biosynthesis by Expressing Glycine max Isoflavone Synthase in Allium cepa L. for Genistein Production. Plants. 2020; 10 (1):52.
Chicago/Turabian StyleAshwini Malla; Balamurugan Shanmugaraj; Balamurugan Srinivasan; Ashutosh Sharma; Sathishkumar Ramalingam. 2020. "Metabolic Engineering of Isoflavonoid Biosynthesis by Expressing Glycine max Isoflavone Synthase in Allium cepa L. for Genistein Production." Plants 10, no. 1: 52.
Superfruit guava (Psidium guajava L.) is one of the healthiest fruits due to its high antioxidant dietary fiber and vitamin content. However, the growth and development of this plant are severely affected by salinity stress, mostly at the seedling stage. MicroRNAs (miRNAs) are small, noncoding, endogenous, highly conserved RNA molecules that play key regulatory roles in plant development, organ morphogenesis, and stress response signaling. In this study, applying computational approaches and following high stringent filtering criteria, a total of 40 potential microRNAs belonging to 19 families were characterized from guava. The identified miRNA precursors formed stable stem-loop structures and exhibited high sequence conservation among diverse and evolutionarily distant plant species. Differential expression pattern of seven selected guava miRNAs (pgu-miR156f-5p, pgu-miR160c-5p, pgu-miR162-3p, pgu-miR164b-5p, pgu-miR166t, pgu-miR167a-5p, and pgu-miR390b-5p) were recorded under salinity stress and pgu-miR162-3p, pgu-miR164b-5p as well as pgu-miR166t were found to be the most affected ones. Using the psRNATarget tool, a total of 49 potential target transcripts of the characterized guava miRNAs were identified in this study which are mostly involved in metabolic pathways, cellular development, and stress response signaling. A biological network has also been constructed to understand the miRNA mediated gene regulation using the minimum free energy (MFE) values of the miRNA-target interaction. To the best of our knowledge, this is the first report of guava miRNAs and their targets.
Ashutosh Sharma; Luis M. Ruiz-Manriquez; Francisco I. Serrano-Cano; Paula Roxana Reyes-Pérez; Cynthia Karina Tovar Alfaro; Yulissa Esmeralda Barrón Andrade; Ana Karen Hernández Aros; Aashish Srivastava; Sujay Paul. Identification of microRNAs and Their Expression in Leaf Tissues of Guava (Psidiumguajava L.) under Salinity Stress. Agronomy 2020, 10, 1920 .
AMA StyleAshutosh Sharma, Luis M. Ruiz-Manriquez, Francisco I. Serrano-Cano, Paula Roxana Reyes-Pérez, Cynthia Karina Tovar Alfaro, Yulissa Esmeralda Barrón Andrade, Ana Karen Hernández Aros, Aashish Srivastava, Sujay Paul. Identification of microRNAs and Their Expression in Leaf Tissues of Guava (Psidiumguajava L.) under Salinity Stress. Agronomy. 2020; 10 (12):1920.
Chicago/Turabian StyleAshutosh Sharma; Luis M. Ruiz-Manriquez; Francisco I. Serrano-Cano; Paula Roxana Reyes-Pérez; Cynthia Karina Tovar Alfaro; Yulissa Esmeralda Barrón Andrade; Ana Karen Hernández Aros; Aashish Srivastava; Sujay Paul. 2020. "Identification of microRNAs and Their Expression in Leaf Tissues of Guava (Psidiumguajava L.) under Salinity Stress." Agronomy 10, no. 12: 1920.
MicroRNAs (miRNAs) are a key gene regulator and play essential roles in several biological and pathological mechanisms in the human system. In recent years, plenty of miRNAs have been identified to be involved in the development of neurodegenerative disorders (NDDs), thus making them an attractive option for therapeutic approaches. Hence, in this review, we provide an overview of the current research of miRNA-based therapeutics for a selected set of NDDs, either for their high prevalence or lethality, such as Alzheimer’s, Parkinson’s, Huntington’s, Amyotrophic Lateral Sclerosis, Friedreich’s Ataxia, Spinal Muscular Atrophy, and Frontotemporal Dementia. We also discuss the relevant delivery techniques, pertinent outcomes, their limitations, and their potential to become a new generation of human therapeutic drugs in the near future.
Sujay Paul; Luis Alberto Bravo Vázquez; Samantha Pérez Uribe; Paula Roxana Reyes-Pérez; Ashutosh Sharma. Current Status of microRNA-Based Therapeutic Approaches in Neurodegenerative Disorders. Cells 2020, 9, 1698 .
AMA StyleSujay Paul, Luis Alberto Bravo Vázquez, Samantha Pérez Uribe, Paula Roxana Reyes-Pérez, Ashutosh Sharma. Current Status of microRNA-Based Therapeutic Approaches in Neurodegenerative Disorders. Cells. 2020; 9 (7):1698.
Chicago/Turabian StyleSujay Paul; Luis Alberto Bravo Vázquez; Samantha Pérez Uribe; Paula Roxana Reyes-Pérez; Ashutosh Sharma. 2020. "Current Status of microRNA-Based Therapeutic Approaches in Neurodegenerative Disorders." Cells 9, no. 7: 1698.
MicroRNAs are endogenous highly conserved short (~ 21 nucleotides) non-coding RNA molecules that play key roles in post-transcriptional gene regulation by translational inhibition or by target mRNA cleavage. In this report, using high stringent computational-based methods, a total of 101 putative miRNAs were identified from European sardine fish (Sardina pilchardus Walbaum, 1792). All the precursors of identified sardine miRNAs formed stable stem-loop structures and displayed high minimum free energy index (MFEI) values. For the experimental validation of the computationally predicted miRNAs, a tissue-specific quantitative study of eight randomly selected putative sardine miRNAs (spi-miR9, spi-miR26, spi-miR128, spi-miR129, spi-miR132, spi-miR212, spi-miR219, and spi-miR338) was performed in brain and liver and all the selected miRNAs were found to be overexpressed in brain tissue. Moreover, using RNAhybrid, a total of 83 potential target proteins of the characterized sardine miRNAs were identified those are involved in transcription, cellular development, defense mechanism, and various signaling pathways. To the best of our knowledge, this is the first report of sardine microRNAs and their targets.
Juan Luis De La Fuente Jiménez; Ashutosh Sharma; Sujay Paul. Characterization of miRNAs from sardine (Sardina pilchardus Walbaum, 1792) and their tissue-specific expression analysis in brain and liver. 3 Biotech 2020, 10, 1 -9.
AMA StyleJuan Luis De La Fuente Jiménez, Ashutosh Sharma, Sujay Paul. Characterization of miRNAs from sardine (Sardina pilchardus Walbaum, 1792) and their tissue-specific expression analysis in brain and liver. 3 Biotech. 2020; 10 (7):1-9.
Chicago/Turabian StyleJuan Luis De La Fuente Jiménez; Ashutosh Sharma; Sujay Paul. 2020. "Characterization of miRNAs from sardine (Sardina pilchardus Walbaum, 1792) and their tissue-specific expression analysis in brain and liver." 3 Biotech 10, no. 7: 1-9.
Triple-negative breast cancer (TNBC) cells are deficient in estrogen, progesterone and ERBB2 receptor expression, presenting a particularly challenging therapeutic target due to their highly invasive nature and relatively low response to therapeutics. There is an absence of specific treatment strategies for this tumor subgroup, and hence TNBC is managed with conventional therapeutics, often leading to systemic relapse. In terms of histology and transcription profile these cancers have similarities to BRCA-1-linked breast cancers, and it is hypothesized that BRCA1 pathway is non-functional in this type of breast cancer. In this review article, we discuss the different receptors expressed by TNBC as well as the diversity of different signaling pathways targeted by TNBC therapeutics, for example, Notch, Hedgehog, Wnt/b-Catenin as well as TGF-beta signaling pathways. Additionally, many epidermal growth factor receptor (EGFR), poly (ADP-ribose) polymerase (PARP) and mammalian target of rapamycin (mTOR) inhibitors effectively inhibit the TNBCs, but they face challenges of either resistance to drugs or relapse. The resistance of TNBC to conventional therapeutic agents has helped in the advancement of advanced TNBC therapeutic approaches including hyperthermia, photodynamic therapy, as well as nanomedicine-based targeted therapeutics of drugs, miRNA, siRNA, and aptamers, which will also be discussed. Artificial intelligence is another tool that is presented to enhance the diagnosis of TNBC.
Mauricio A. Medina; Goldie Oza; Ashutosh Sharma; L.G. Arriaga; José Manuel Hernández Hernández; Vincent M. Rotello; Jose Tapia Ramirez. Triple-Negative Breast Cancer: A Review of Conventional and Advanced Therapeutic Strategies. International Journal of Environmental Research and Public Health 2020, 17, 2078 .
AMA StyleMauricio A. Medina, Goldie Oza, Ashutosh Sharma, L.G. Arriaga, José Manuel Hernández Hernández, Vincent M. Rotello, Jose Tapia Ramirez. Triple-Negative Breast Cancer: A Review of Conventional and Advanced Therapeutic Strategies. International Journal of Environmental Research and Public Health. 2020; 17 (6):2078.
Chicago/Turabian StyleMauricio A. Medina; Goldie Oza; Ashutosh Sharma; L.G. Arriaga; José Manuel Hernández Hernández; Vincent M. Rotello; Jose Tapia Ramirez. 2020. "Triple-Negative Breast Cancer: A Review of Conventional and Advanced Therapeutic Strategies." International Journal of Environmental Research and Public Health 17, no. 6: 2078.
MicroRNAs (miRNAs) are highly conserved, short (~ 21-nucleotide), endogenous, non-coding RNA molecules that play major roles in post-transcriptional silencing by guiding target mRNA cleavage or translational inhibition. In this study, applying high-stringent genome-wide computational-based approaches, a total of 28 putative miRNAs belonging to 17 miRNA families were identified from an antioxidant-rich medicinal plant passion fruit (Passiflora edulis). Inter-tissue (leaves and fruits) and inter-varietal (yellow and purple fruit varieties) quantitative study of six putative passion fruit miRNAs (ped-miR160, ped-miR164, ped-miR166, ped-miR393, ped-miR394, and ped-miR398) showed differential expression. Using psRNATarget tool, a total of 25 potential target proteins of the characterized passion fruit miRNAs were also identified. Most of the target proteins identified in this study, including SQUAMOSA promoter binding, Class III HD-Zip, NAC, Scarecrow, APETALA2, Auxin response factor, MYB, and superoxide dismutase, were found to be involved in development, metabolism, and defense/stress response signaling.
Sujay Paul; Juan Luis De La Fuente-Jiménez; Camila Garibay Manriquez; Ashutosh Sharma. Identification, characterization and expression analysis of passion fruit (Passiflora edulis) microRNAs. 3 Biotech 2020, 10, 1 -9.
AMA StyleSujay Paul, Juan Luis De La Fuente-Jiménez, Camila Garibay Manriquez, Ashutosh Sharma. Identification, characterization and expression analysis of passion fruit (Passiflora edulis) microRNAs. 3 Biotech. 2020; 10 (1):1-9.
Chicago/Turabian StyleSujay Paul; Juan Luis De La Fuente-Jiménez; Camila Garibay Manriquez; Ashutosh Sharma. 2020. "Identification, characterization and expression analysis of passion fruit (Passiflora edulis) microRNAs." 3 Biotech 10, no. 1: 1-9.
Sangita Chowdhury Paul; Ashutosh Sharma; Richa Mehta; Sujay Paul. In silico Characterization of microRNAs and Their Target Transcripts from Cranberry (Vaccinium macrocarpon). Cytology and Genetics 2020, 54, 82 -90.
AMA StyleSangita Chowdhury Paul, Ashutosh Sharma, Richa Mehta, Sujay Paul. In silico Characterization of microRNAs and Their Target Transcripts from Cranberry (Vaccinium macrocarpon). Cytology and Genetics. 2020; 54 (1):82-90.
Chicago/Turabian StyleSangita Chowdhury Paul; Ashutosh Sharma; Richa Mehta; Sujay Paul. 2020. "In silico Characterization of microRNAs and Their Target Transcripts from Cranberry (Vaccinium macrocarpon)." Cytology and Genetics 54, no. 1: 82-90.
Staphylococcus epidermidis is a coagulase-negative bacterium capable of causing recurrent relapses in prosthetic joint infection (PJI). The aim of this study was to determine if Staphylococcus epidermidis isolates from patients with recurrent relapses of prosthetic joint infection (PJI) changed genotypically (pulsed-field gel electrophoresis (PFGE) pattern analysis and genes involved in biofilm formation) and phenotypically (antimicrobial resistance, biofilm formation) during the different episodes. Four patients with PJI recurrent relapses were evaluated clinically and microbiologically. Genotypic and phenotypic characteristics of 31 S. epidermidis isolates were determined. In all cases, PJI was treated with antimicrobial therapy and resection of the prosthesis without reimplantation. Months later, all patients had a relapse episode and treated with rifampin plus vancomycin and surgical debridement. Changes in the antibiotics resistance profile in isolates from patients 1 and 2 were observed in the two episodes. Patient 1 had four clones A, B, C, and D that were distributed differentially in the two episodes. Similarly, patients 2 and 3 had two clones and subclones (E-E1 and F-F1, respectively), and patient 4 had only the clone G in both episodes. The clone F formed small-colony variants (SCVs). High level of biofilm formation was found in all clones, except for clones D and G. Clones/subclones showed a genotypic variation in icaA, sdrF, bap, sesI, and embp genes. The principal coordinate analysis showed that all clones/subclones were different. These results showed that the initial infective clone of S. epidermidis from PJI, changed genotypically and phenotypically after a second relapse as a response to the treatment.
Silvestre Ortega-Peña; Rafael Franco-Cendejas; Alejandra Aquino-Andrade; Gabriel Betanzos-Cabrera; Ashutosh Sharma; Sandra Rodríguez-Martínez; Mario E. Cancino-Diaz; Juan Carlos Cancino-Diaz. Genotypic and phenotypic changes of Staphylococcus epidermidis during relapse episodes in prosthetic joint infections. Brazilian Journal of Microbiology 2019, 51, 601 -612.
AMA StyleSilvestre Ortega-Peña, Rafael Franco-Cendejas, Alejandra Aquino-Andrade, Gabriel Betanzos-Cabrera, Ashutosh Sharma, Sandra Rodríguez-Martínez, Mario E. Cancino-Diaz, Juan Carlos Cancino-Diaz. Genotypic and phenotypic changes of Staphylococcus epidermidis during relapse episodes in prosthetic joint infections. Brazilian Journal of Microbiology. 2019; 51 (2):601-612.
Chicago/Turabian StyleSilvestre Ortega-Peña; Rafael Franco-Cendejas; Alejandra Aquino-Andrade; Gabriel Betanzos-Cabrera; Ashutosh Sharma; Sandra Rodríguez-Martínez; Mario E. Cancino-Diaz; Juan Carlos Cancino-Diaz. 2019. "Genotypic and phenotypic changes of Staphylococcus epidermidis during relapse episodes in prosthetic joint infections." Brazilian Journal of Microbiology 51, no. 2: 601-612.
MicroRNAs (miRNAs) are short, endogenous, noncoding RNAs that regulate gene expression through posttranscriptional mechanisms via degradation or inhibition of specific mRNAs targets. In recent years, abundant studies have illustrated the relevance of miRNAs in human psychopathology. In this current review, neuropsychiatric disorders with moderate to high prevalence among children and adolescents such as Autism Spectrum Disorder, Attention-Deficit/Hyperactivity Disorder, Dyslexia, Epilepsy, Schizophrenia and Tourette Syndrome were discussed focusing on the functional consequence of altered miRNA expression during the development of such diseases. The insight about the roles that miRNAs play in central nervous systems development such as cell proliferation and differentiation, synaptogenesis, synaptic plasticity, and apoptosis might be the key to explicate novel biomarkers for diagnosis and prognosis of these disorders, as well as the finding of new targets for drug development for therapeutic approaches.
Sujay Paul; Paula Roxana Reyes; Betsabé Sánchez Garza; Ashutosh Sharma. MicroRNAs and Child Neuropsychiatric Disorders: A Brief Review. Neurochemical Research 2019, 45, 232 -240.
AMA StyleSujay Paul, Paula Roxana Reyes, Betsabé Sánchez Garza, Ashutosh Sharma. MicroRNAs and Child Neuropsychiatric Disorders: A Brief Review. Neurochemical Research. 2019; 45 (2):232-240.
Chicago/Turabian StyleSujay Paul; Paula Roxana Reyes; Betsabé Sánchez Garza; Ashutosh Sharma. 2019. "MicroRNAs and Child Neuropsychiatric Disorders: A Brief Review." Neurochemical Research 45, no. 2: 232-240.
Current knowledge in the use of hairy root cell cultures is growing. The use of this technology in the production of secondary metabolites is also increasing with the general purpose of generating medicinal alternatives from ethnopharmaceutical origins. Strategies include either genetically modified hairy roots that overexpress genes that codify for key enzymes in complex metabolic pathways or the use of certain elicitors for the natural production of certain compounds in hairy roots. The importance of understanding basic root technology and the complex genetic regulation of root development helps in establishing well-designed strategies in order to obtain higher yields in terms of metabolites or recombinant protein production. Also, bioprocess factors such as the use of bioreactors, elicitors, nutrients, and phytohormones for the induction of compounds of economic importance are also growing. Thus, the aim of the present chapter is to summarize the most important aspects regarding the technology to generate these hairy roots and their main applications in novel fields such as phytoremediation.
Paola Isabel Angulo-Bejarano; Juan Luis De La Fuente Jimenez; Sujay Paul; Marcos De Donato-Capote; Irais Castillo-Maldonado; Gabriel Betanzos-Cabrera; Juan Ignacio Valiente-Banuet; Ashutosh Sharma. Cell Cultures and Hairy Roots as Platform for Production of High-Value Metabolites: Current Approaches, Limitations, and Future Prospects. Advances in Plant Transgenics: Methods and Applications 2019, 23 -57.
AMA StylePaola Isabel Angulo-Bejarano, Juan Luis De La Fuente Jimenez, Sujay Paul, Marcos De Donato-Capote, Irais Castillo-Maldonado, Gabriel Betanzos-Cabrera, Juan Ignacio Valiente-Banuet, Ashutosh Sharma. Cell Cultures and Hairy Roots as Platform for Production of High-Value Metabolites: Current Approaches, Limitations, and Future Prospects. Advances in Plant Transgenics: Methods and Applications. 2019; ():23-57.
Chicago/Turabian StylePaola Isabel Angulo-Bejarano; Juan Luis De La Fuente Jimenez; Sujay Paul; Marcos De Donato-Capote; Irais Castillo-Maldonado; Gabriel Betanzos-Cabrera; Juan Ignacio Valiente-Banuet; Ashutosh Sharma. 2019. "Cell Cultures and Hairy Roots as Platform for Production of High-Value Metabolites: Current Approaches, Limitations, and Future Prospects." Advances in Plant Transgenics: Methods and Applications , no. : 23-57.