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Dr. Antonina Kruglova
Aalto University

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0 Activated Sludge
0 Antibiotic Resistance
0 Biodegradation
0 Wastewater Treatment
0 antibiotics

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Journal article
Published: 31 August 2020 in Water
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In this study, the microbial community of nitrifying activated sludge adapted to Finnish climate conditions was studied to clarify the microbial populations involved in low-temperature nitrification. Microbial community analysis of five full-scale wastewater treatment plants (WWTPs) showed several differences compared to WWTPs from other countries with a similar climate. In particular, very low abundance of ammonium oxidizing bacteria (AOBs) (altogether ˂ 0.25% of total community) as well as typical NOBs (˂0.35%) and a high abundance of orders Cytophagales and Micrococcales was observed in all Finnish WWTPs. To shed light on the importance of autotrophic and heterotrophic nitrifying processes, laboratory studies of activated sludge were carried out with a presence of and a lack of organic carbon in wastewater at 10 ± 1 °C. Two different sludge retention times (SRTs) were compared to determine the effect of this operational parameter on low-temperature nitrogen removal. The important role of previously reported Candidatus Nitrotogaarctica for nitrite oxidizing in cold climate conditions was confirmed in both full-scale and laboratory scale results. Additionally, potential participation of Dokdonella sp. and Flexibacter sp. in nitrogen removal at low-temperatures is proposed. Operation at SRT of 100 days demonstrated more stable and efficient nitrogen removal after a sharp temperature decrease compared to 14 days SRT.

ACS Style

Antonina Kruglova; Jenni Kesulahti; Khoi Minh Le; Alejandro Gonzalez-Martinez; Anna Mikola; Riku Vahala. Low-Temperature Adapted Nitrifying Microbial Communities of Finnish Wastewater Treatment Systems. Water 2020, 12, 2450 .

AMA Style

Antonina Kruglova, Jenni Kesulahti, Khoi Minh Le, Alejandro Gonzalez-Martinez, Anna Mikola, Riku Vahala. Low-Temperature Adapted Nitrifying Microbial Communities of Finnish Wastewater Treatment Systems. Water. 2020; 12 (9):2450.

Chicago/Turabian Style

Antonina Kruglova; Jenni Kesulahti; Khoi Minh Le; Alejandro Gonzalez-Martinez; Anna Mikola; Riku Vahala. 2020. "Low-Temperature Adapted Nitrifying Microbial Communities of Finnish Wastewater Treatment Systems." Water 12, no. 9: 2450.

Research article
Published: 09 October 2018 in Biotechnology Progress
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The effect of antibiotics sulfadiazine and trimethoprim on activated sludge operated at 8 °C was investigated. Performance and microbial communities of sequencing batch reactors (SBR) and Membrane Bioreactors (MBR) were compared before and after the exposure of antibiotics to the synthetic wastewater. The results revealed irreversible negative effect of these antibiotics in environmentally relevant concentrations on nitrifying microbial community of SBR activated sludge. In opposite, MBR sludge demonstrated fast adaptation and more stable performance during the antibiotics exposure. Dynamics of microbial community was greatly affected by presence of antibiotics. Bacteria from classes Betaproteobacteria and Bacteroidetes demonstrated the potential to develop antibiotic resistance in both wastewater treatment systems whilst Actinobacteria disappeared from all of the reactors after 60 days of antibiotics exposure. Altogether, results showed that operational parameters such as sludge retention time (SRT) and reactor configuration had great effect on microbial community composition of activated sludge and its vulnerability to antibiotics. Operation at long SRT allowed archaea, including ammonium oxidizing species (AOA) such as Nitrososphaera viennensis to grow in MBRs. AOA could have an important role in stable nitrification performance of MBR activated sludge due to tolerance of archaea to antibiotics. This article is protected by copyright. All rights reserved.

ACS Style

Antonina Kruglova; Anna Mikola; Alejandro Gonzalez-Martinez; Riku Vahala. Effect of sulfadiazine and trimethoprim on activated sludge performance and microbial community dynamics in laboratory-scale membrane bioreactors and sequencing batch reactors at 8°C. Biotechnology Progress 2018, 35, e2708 .

AMA Style

Antonina Kruglova, Anna Mikola, Alejandro Gonzalez-Martinez, Riku Vahala. Effect of sulfadiazine and trimethoprim on activated sludge performance and microbial community dynamics in laboratory-scale membrane bioreactors and sequencing batch reactors at 8°C. Biotechnology Progress. 2018; 35 (1):e2708.

Chicago/Turabian Style

Antonina Kruglova; Anna Mikola; Alejandro Gonzalez-Martinez; Riku Vahala. 2018. "Effect of sulfadiazine and trimethoprim on activated sludge performance and microbial community dynamics in laboratory-scale membrane bioreactors and sequencing batch reactors at 8°C." Biotechnology Progress 35, no. 1: e2708.

Journal article
Published: 01 December 2017 in Science of The Total Environment
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In this study the influence of low-temperature (8°C), sludge retention time (SRT) and loading of spotlight wastewater micropollutants (MPs) on bacterial community of activated sludge was investigated with a special focus on nitrification. Two Sequencing batch reactors (SBR) and two membrane bioreactors (MBR) were operated with synthetic municipal-like wastewater receiving and not receiving ibuprofen, diclofenac, estrone and 17α-ethynylestradiol (EE2). Bacterial population studies were related to removal efficiencies of studied MPs. The results showed that studied bacterial communities significantly differed from all previously published nitrifying activated sludge communities. Exceptionally low concentration of autotrophic nitrifying bacteria were found (<0.5%) as well as no common heterotrophic nitrifies were presenting in activated sludge and therefore could not be related to the MPs removal. Additionally SRT had a spacious effect on the diversity of bacteria and bacterial population shifts under pressure of MPs. Growth of Firmicutes was suppressed by presence of MPs in all the reactors. Increase of MPs concentrations in wastewater improved the removal of EE2. Abundance of Delta- and Gammaproteobacteria showed positive correlation with diclofenac removal.

ACS Style

Antonina Kruglova; Alejandro Gonzalez-Martinez; Matilda Kråkström; Anna Mikola; Riku Vahala. Bacterial diversity and population shifts driven by spotlight wastewater micropollutants in low-temperature highly nitrifying activated sludge. Science of The Total Environment 2017, 605-606, 291 -299.

AMA Style

Antonina Kruglova, Alejandro Gonzalez-Martinez, Matilda Kråkström, Anna Mikola, Riku Vahala. Bacterial diversity and population shifts driven by spotlight wastewater micropollutants in low-temperature highly nitrifying activated sludge. Science of The Total Environment. 2017; 605-606 ():291-299.

Chicago/Turabian Style

Antonina Kruglova; Alejandro Gonzalez-Martinez; Matilda Kråkström; Anna Mikola; Riku Vahala. 2017. "Bacterial diversity and population shifts driven by spotlight wastewater micropollutants in low-temperature highly nitrifying activated sludge." Science of The Total Environment 605-606, no. : 291-299.

Review
Published: 01 May 2017 in International Journal of Hygiene and Environmental Health
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Antibiotics are found globally in the environment at trace levels due to their extensive consumption, which raises concerns about the effects they can have on non-target organisms, especially environmental micro-organisms. So far the majority of studies have focused on different aspects of antibiotic resistance or on analyzing the occurrence, fate, and removal of antibiotics from hospital and municipal wastewaters. Little attention has been paid to ecotoxicological effects of antibiotics on aquatic micro-organisms although they play a critical role in most ecosystems and they are potentially sensitive to these substances. Here we review the current state of research on the toxicological impacts of antibiotics to aquatic micro-organisms, including proteobacteria, cyanobacteria, algae and bacteria commonly present in biological wastewater treatment processes. We focus on antibiotics that are poorly removed during wastewater treatment and thus end up in surface waters. We critically discuss and compare the available analytical methods and test organisms based on effect concentrations and identify the knowledge gaps and future challenges. We conclude that, in general, cyanobacteria and ammonium oxidizing bacteria are the most sensitive micro-organisms to antibiotics. It is important to include chronic tests in ecotoxicological assessment, because acute tests are not always appropriate in case of low sensitivity (for example for proteobacteria). However, the issue of rapid development of antibiotic resistance should be regarded in chronic testing. Furthermore, the application of other species of bacteria and endpoints should be considered in the future, not forgetting the mixture effect and bacterial community studies. Due to differences in the sensitivity of different test organisms to individual antibiotic substances, the application of several bioassays with varying test organisms would provide more comprehensive data for the risk assessment of antibiotics. Regardless of the growing concerns related to antibiotics in the environment, there are still evident knowledge gaps related to antibiotics, as there is only limited or no ecotoxicological data on many potentially harmful antibiotics.

ACS Style

Pia Välitalo; Antonina Kruglova; Anna Mikola; Riku Vahala. Toxicological impacts of antibiotics on aquatic micro-organisms: A mini-review. International Journal of Hygiene and Environmental Health 2017, 220, 558 -569.

AMA Style

Pia Välitalo, Antonina Kruglova, Anna Mikola, Riku Vahala. Toxicological impacts of antibiotics on aquatic micro-organisms: A mini-review. International Journal of Hygiene and Environmental Health. 2017; 220 (3):558-569.

Chicago/Turabian Style

Pia Välitalo; Antonina Kruglova; Anna Mikola; Riku Vahala. 2017. "Toxicological impacts of antibiotics on aquatic micro-organisms: A mini-review." International Journal of Hygiene and Environmental Health 220, no. 3: 558-569.

Comparative study
Published: 01 August 2016 in Bioresource Technology
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Four emerging micropollutants ibuprofen, diclofenac, estrone (E1) and 17α-ethinylestradiol (EE2) were studied in large laboratory-scale wastewater treatment plants (WWTPs) with high nitrifying activity. Activated sludge (AS) with sludge retention times (SRTs) of 12 days and 14 days in sequencing batch reactors (SBRs) and 30 days, 60 days and 90 days in membrane bioreactors (MBRs) were examined at 8 °C and 12 °C. Concentrations of pharmaceuticals and their main metabolites were analysed in liquid phase and solid phase of AS by liquid chromatography–tandem mass spectrometry (LC–MS/MS). A remarkable amount of contaminants were detected in solids of AS, meaning the accumulation of micropollutants in bacterial cells. The biodegradation rate constants (Kbiol) were affected by SRT and temperature. MBR with a 90-day SRT showed the best results of removal. Conventional SBR process was inefficient at 8 °C showing Kbiol values lower than 0.5 l gSS−1 d−1 for studied micropollutants.

ACS Style

Antonina Kruglova; Matilda Kråkström; Mats Riska; Anna Mikola; Pirjo Rantanen; Riku Vahala; Leif Kronberg. Comparative study of emerging micropollutants removal by aerobic activated sludge of large laboratory-scale membrane bioreactors and sequencing batch reactors under low-temperature conditions. Bioresource Technology 2016, 214, 81 -88.

AMA Style

Antonina Kruglova, Matilda Kråkström, Mats Riska, Anna Mikola, Pirjo Rantanen, Riku Vahala, Leif Kronberg. Comparative study of emerging micropollutants removal by aerobic activated sludge of large laboratory-scale membrane bioreactors and sequencing batch reactors under low-temperature conditions. Bioresource Technology. 2016; 214 ():81-88.

Chicago/Turabian Style

Antonina Kruglova; Matilda Kråkström; Mats Riska; Anna Mikola; Pirjo Rantanen; Riku Vahala; Leif Kronberg. 2016. "Comparative study of emerging micropollutants removal by aerobic activated sludge of large laboratory-scale membrane bioreactors and sequencing batch reactors under low-temperature conditions." Bioresource Technology 214, no. : 81-88.

Journal article
Published: 01 November 2014 in Science of The Total Environment
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Pharmaceuticals constitute a well-known group of emerging contaminants with an increasing significance in water pollution. This study focuses on three pharmaceuticals extensively used in Finland and which can be found in environmental waters: ibuprofen, diclofenac and carbamazepine. Biodegradation experiments were conducted in a full-scale Wastewater Treatment Plant (WWTP) and in laboratory-scale Sequencing Batch Reactors (SBRs). The SBRs were operated at 12 °C, with a sludge retention time (SRT) 10-12 d and organic loading rates (OLRs) of 0.17, 0.27 and 0.33 kg BOD7 m(-3) d(-1). Ibuprofen was found to biodegrade up to 99%. The biodegradation rate constants (k(biol)) for ibuprofen were calculated for full-scale and laboratory processes as well as under different laboratory conditions and found to differ from 0.9 up to 5.0 l g(SS)(-1) d(-1). Diclofenac demonstrated an unexpected immediate drop of concentration in three SBRs and partial recovery of the initial concentration in one of the reactors. High fluctuating in diclofenac concentration was presumably caused by removal of this compound under different concentrations of nitrites during development of nitrifying activated sludge. Carbamazepine showed no biodegradation in all the experiments.

ACS Style

Antonina Kruglova; Pia Ahlgren; Nasti Korhonen; Pirjo-Liisa Rantanen; Anna Mikola; Riku Vahala. Biodegradation of ibuprofen, diclofenac and carbamazepine in nitrifying activated sludge under 12°C temperature conditions. Science of The Total Environment 2014, 499, 394 -401.

AMA Style

Antonina Kruglova, Pia Ahlgren, Nasti Korhonen, Pirjo-Liisa Rantanen, Anna Mikola, Riku Vahala. Biodegradation of ibuprofen, diclofenac and carbamazepine in nitrifying activated sludge under 12°C temperature conditions. Science of The Total Environment. 2014; 499 ():394-401.

Chicago/Turabian Style

Antonina Kruglova; Pia Ahlgren; Nasti Korhonen; Pirjo-Liisa Rantanen; Anna Mikola; Riku Vahala. 2014. "Biodegradation of ibuprofen, diclofenac and carbamazepine in nitrifying activated sludge under 12°C temperature conditions." Science of The Total Environment 499, no. : 394-401.