Barium titanate (BaTiO₃) nanoparticles (BTNPs) have been considered as emerging materials in biomedical sector through last decades due to the excellent physicochemical properties such as dielectric and piezoelectric structures, biocompatibility, and nonlinear optical characteristics. In this study, BTNPs were synthesized via the co-precipitation method using barium carbonate and titanium dioxide by stirring for 5 h. Then, it was annealed at 850 °C for 5 h with five different concentrations: 0.2, 0.4, 0.6, 0.8, and 1 g/mL. The structural, morphological, and optical analyses were demonstrated by different characterization techniques such as X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive X-ray (EDX), thermogravimetric analysis (TGA), Raman, and UV–visible spectroscopy. The perovskite phase of BTNPs, an intense peak at 31.6°, was observed at the lowest concentration (0.2 g/mL), and the average crystalline size was 1.42 nm based on XRD pattern. The results have been justified by SEM and EDX. TGA demonstrated the adequate thermal stability of this material. EDX analysis confirmed the composition of Ti, Ba, and O elements. Raman peaks at 305 cm⁻¹ and 517 cm⁻¹ confirmed the formation of BaTiO₃. UV–visible spectra presented that its’ absorbance edge shifted into visible range at 404 nm. Application of BTNPs on breast cancer cell line (MCF-7) presented significant dispersion effect at 0.2, 0.4 and 0.6 g/mL of BaTiO₃. A strong toxicity rate of BaTiO₃ has been observed against the MCF-7 cell line. Maximum % of cell viability loss, $$\cong$$≅ 57% was recorded at 200 µg/mL of BTNPs, and minimum % of cell viability loss was observed as 19% at 50 µg/mL of BTNPs. The results presented that a higher concentration of BTPNs dosage was more effective in inhibition of breast cancer cells. Therefore, BTNPs can be recommended as a promising nanomaterial for anti-cancer drug discovery.

In this experimental approach, we explored the structures, morphologies, phototoxicities, and antibacterial activities of undoped and Mn-doped ceria nanocomposite materials, Mn_xCe_1−xO_2. The Mn_xCe_1−xO₂ nanocomposites were synthesized by employing a soft chemical route. Our prime focus was on the influence of different factors, both physical and chemical, i.e., the concentration of manganese in the product, size of the nanocomposite, drug dose, and incubation time, on the bacterial strains. Different bacterial strains were selected as experimental biological models of the antibacterial activity of the manganese-doped cerium oxide nanocomposite. In addition to the photodynamic response, the adenocarcinoma cell line (MCF-7) was also studied. Based on cell viability losses and bacterial inhibition analyses, the precise mechanisms of apoptosis or necrosis of 5-ALA/PpIX-exposed MCF-7 cells under 630 nm red lights and under dark conditions were elucidated. It was observed that the undoped nanocomposites had lower cytotoxicities and inhibitions compared with those of the doped nanocomposites towards pathogens. The antibacterial activity and effectiveness for photodynamic therapy were enhanced in the presence of the manganese-doped ceria nanocomposite, which could be attributed to the correlation of the maximum reactive oxygen species generation for targeted toxicity and maximum antioxidant property in bacteria growth inhibition. The optimized cell viability dose and doping concentration will be beneficial for treating cancer and bacterial infections in the future.

Significant apoptotic effect in Hep2c cell line has been investigated, when diode laser (λ = 635 nm, red) are used as a source of illumination and initiation of photodynamic action. The optimal uptake time of Photofrin® for Hep2c cell line was investigated by means of spectrophotometric measurement. Quantification of the live cell population was determined by means of neutral red assay (NRA). The spectrometry measurements showed that after 46 h incubation, the maximal cellular uptake of Photofrin® was achieved and photocytotoxic assay showed that light dose of 120 J/cm² give effective PDT outcome for Hep2c cell line incubated with 85 μg/ml of Photofrin®. No significant phototoxic and cytotoxic effects on Hep2c cells were observed due to light doses or photosensitize, when studied independently of each other and Photofrin® showed good anti tumor effects.

The preparation of a manganese-doped cerium oxide (Mn:CeO₂) nanocomposite via hydrothermal route is described. Cubic fluorite structure of single phase was exhibited by studying structural analysis through x-ray diffraction (XRD) technique and morphological analysis was conducted by scanning electron microscope. Surface analytic technique of energy dispersive x-ray spectroscopy (EDX) was conducted to analyze the relative amount of any impurity and doping. Structural changes due to manganese doping such as increment in production of vacancies of oxygen within crystal of cerium oxide, and reduction in size of crystallite and constant of lattice was observed in our research study. Moreover, the Mn:CeO₂ nanocomposite demonstrates differential cytotoxicity against MCF-7 adenocarcinoma cell line, which renders it a promising candidate for targeted cancer therapy. The anti-tumorous activity of the cerium oxide nanocomposite was significantly enhanced with doping of manganese, which is directly linked with the generation of highly reactive oxygen facets. The experimental results are supported by a mathematical model that confirms a confidence level of 95%. This research has paved the way for many utilities in therapeutics and magnetic resonance imaging diagnostics through new observations, and hence verified their math model.

Graphene-based materials have garnered significant attention because of their versatile bioapplications and extraordinary properties. Graphene oxide (GO) is an extremely oxidized form of graphene accompanied by the functional groups of oxygen on its surface. GO is an outstanding platform on which to pacify silver nanoparticles (Ag NPs), which gives rise to the graphene oxide-silver nanoparticle (GO-Ag) nanocomposite. In this experimental study, the toxicity of graphene oxide-silver (GO-Ag) nanocomposites was assessed in an in vitro human breast cancer model to optimize the parameters of photodynamic therapy. GO-Ag was prepared using the hydrothermal method, and characterization was done by X-ray diffraction, field-emission scanning electron microscope (FE-SEM), transmission Electron Microscopy (TEM), energy dispersive X-rays Analysis (EDAX), atomic force microscopy and ultraviolet-visible spectroscopy. The experiments were done both with laser exposure, as well as in darkness, to examine the phototoxicity and cytotoxicity of the nanocomposites. The cytotoxicity of the GO-Ag was confirmed via a methyl-thiazole-tetrazolium (MTT) assay and intracellular reactive oxygen species production analysis. The phototoxic effect explored the dose-dependent decrease in the cell viability, as well as provoked cell death via apoptosis. An enormously significant escalation of ¹O₂ in the samples when exposed to daylight was perceived. Statistical analysis was performed on the experimental results to confirm the worth and clarity of the results, with p-values < 0.05 selected as significant. These outcomes suggest that GO-Ag nanocomposites could serve as potential candidates for targeted breast cancer therapy.