Ship emulsified oil wastewater was used as the research object in this study. The highly efficient coagulant demulsification degreasing mechanism and microbubble flotation technology were combined and the effects of coagulant type and dosage amount on the demulsification of emulsified oil wastewater were evaluated. The influence of the mixed coagulation effect of pH values, temperature, and hydraulic condition parameters were determined and water intake, air intake, and oil content were regulated. The coagulant for the demulsification of emulsified oil wastewater was screened; the dosage was 500 mg L⁻¹, and the removal capacity of the coagulant was in the following order: polyaluminum ferric chloride (PAFC) > polyaluminum chloride (PAC) > polysilicate aluminum ferric sulfate (PSAFS) > alum > Al₂(SO₄)₃ > polyferric sulfate > FeCl₃. Polyacrylamide (PAM) with added water was used to further reduce the oil content. The PAFC, PAC, and PSAFS were selected as coagulation–air flotation dynamic test alternative agents. The investment quantities of PAFC, PSAFS and PAM were 300 mg L⁻¹, 300 mg L⁻¹ and 30 mg L⁻¹, respectively. The stirring time was 5 min, the pH value was 6.5–6.9, the flow rate was 0.25 m³ h⁻¹, the oil content of the emulsified oil wastewater was 3000–5000 mg L⁻¹ and the effluent oil was stable below 15 ppm. The microbubble generation device using air flotation effluent was used in the two air flotation treatments to enhance the device efficiency. The air flotation device adopted the structural design of the upper part of the water inlet and the lower part of the micro-air bubble, which can increase the collision probability of the microbubble and improve the efficiency of oil removal.

The aim of this work was to develop bacterial communities to effectively degrade petroleum hydrocarbons (PHs). We investigated the biotic and abiotic contributors to differences in PHs degradation efficacy between two bacterial strains, Klebsiella pneumoniae (Kp) and Pseudomonas aeruginosa (Pa), screened out from the activated sludge of a petroleum refinery. We characterized the temporal variations in degradation efficacy for diesel and its five major constituents as a sole carbon source and identified more constituents they degraded. The growth characteristics, surface tension, hydrophobicity and emulsifiability of these two strains were measured. We further estimated the relationships between their degradation efficacy and all the biotic and abiotic factors. Results showed that the Pa strain had higher diesel degradation efficacy (58% on Day 14) and utilized more diesel constituents (86%) compared to Kp. Additionally, the growth of the Pa strain in diesel medium was faster than that of the Kp strain. The Pa strain had a lower surface tension and higher hydrophobicity and emulsifiability than Kp, while the surfactant produced by Pa was identified as rhamnolipids. Degradation of PHs was positively related to bacterial growth, hydrophobicity and emulsification but negatively related to surface tension. Overall, differences in degrading capacity for diesel constituents, relative growth rate, and biosurfactant production contributed to the variation in the PHs degradation efficacy of these two bacterial strains.

In this study, effects of pretreatment of corn stover (CS) with sodium hydroxide (NaOH) combined with calcium oxide (CaO) on anaerobic co-digestion of swine manure and CS for biogas production were investigated. Different pretreated-CSs were prepared by adding different doses of NaOH and CaO to CS: Treat-CSA (0.10 g NaOH/g CS), Treat-CSB ((0.075 g NaOH + 0.05 g CaO)/g CS), Treat-CSC ((0.05 g NaOH + 0.05 g CaO)/g CS), and Treat-CSD ((0.025 g NaOH + 0.1 g CaO)/g CS). Lignin removal rate, biomass recovery, reduced sugar, methane yield, DT₈₀ (digestion time when biogas achieved 80% of the total biogas), composition of residues, and cost-efficiency were measured to characterize CS after pretreatment and to evaluate the performance of co-digestors fed with swine manure and differently-pretreated CS. The results showed that Treat-CSB showed an excellent lignin removal efficiency and biomass recovery, resulting in the highest methane yield in its co-digestion with swine manure. Since the net benefit of Treat-CSB was calculated to be the highest (i.e., $1.89/ton total solids), therefore, we believe the co-digestion of Treat-CSB and swine manure for biogas production be an effective valorization option for the wastes.