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Royal jelly (RJ) is a complex, creamy secretion produced by the glands of worker bees. Due to its health-promoting properties, it is used by humans as a dietary supplement. However, RJ compounds are not fully characterized yet. Hence, in this research, we aimed to broaden the knowledge of the proteomic composition of fresh RJ. Water extracts of the samples were pre-treated using combinatorial hexapeptide ligand libraries (ProteoMinerTM kit), trypsin-digested, and analyzed by a nanoLC-MALDI-TOF/TOF MS system. To check the ProteoMinerTM performance in the MS-based protein identification, we also examined RJ extracts that were not prepared with the ProteoMinerTM kit. We identified a total of 86 proteins taxonomically classified to Apis spp. (bees). Among them, 74 proteins were detected in RJ extracts pre-treated with ProteoMinerTM kit, and only 50 proteins were found in extracts non-enriched with this technique. Ten of the identified features were hypothetical proteins whose existence has been predicted, but any experimental evidence proves their in vivo expression. Additionally, we detected four uncharacterized proteins of unknown functions. The results of this research indicate that the ProteoMinerTM strategy improves proteomic identification in complex biological samples. Broadening the knowledge of RJ composition may contribute to the development of standards and regulations, enhancing the quality of RJ, and consequently, the safety of its supplementation.
Eliza Matuszewska; Joanna Matysiak; Grzegorz Rosiński; Elżbieta Kędzia; Weronika Ząbek; Jarosław Zawadziński; Jan Matysiak. Mining the Royal Jelly Proteins: Combinatorial Hexapeptide Ligand Library Significantly Improves the MS-Based Proteomic Identification in Complex Biological Samples. Molecules 2021, 26, 2762 .
AMA StyleEliza Matuszewska, Joanna Matysiak, Grzegorz Rosiński, Elżbieta Kędzia, Weronika Ząbek, Jarosław Zawadziński, Jan Matysiak. Mining the Royal Jelly Proteins: Combinatorial Hexapeptide Ligand Library Significantly Improves the MS-Based Proteomic Identification in Complex Biological Samples. Molecules. 2021; 26 (9):2762.
Chicago/Turabian StyleEliza Matuszewska; Joanna Matysiak; Grzegorz Rosiński; Elżbieta Kędzia; Weronika Ząbek; Jarosław Zawadziński; Jan Matysiak. 2021. "Mining the Royal Jelly Proteins: Combinatorial Hexapeptide Ligand Library Significantly Improves the MS-Based Proteomic Identification in Complex Biological Samples." Molecules 26, no. 9: 2762.
Hymenoptera venom allergy significantly affects the quality of life. Due to the divergences in the results of the available test and clinical symptoms of patients, the current widely applied diagnostic methods are often insufficient to classify patients for venom immunotherapy (VIT). Therefore it is still needed to search for new, more precise, and accurate diagnostic methods. Hence, this research aimed to discover new biomarkers of Hymenoptera venom allergy in a group of inflammation factors using set of multi-marker Bioplex panel. The adoption of a novel methodology based on Luminex/xMAP enabled simultaneous determination of serum levels of 37 different inflammatory proteins in one experiment. The study involved 21 patients allergic to wasp and/or honey bee venom and 42 healthy participants. According to univariate and multivariate statistics, soluble CD30/tumor necrosis factor receptor superfamily, member 8 (sCD30/TNFRSF8), and the soluble tumor necrosis factor receptor 1 (sTNF-R1) may be considered as effective prognostic factors, their circulating levels were significantly decreased in the allergy group (p-value < 0.05; the Area Under the Curve (AUC) ~0.7; Variable Importance in Projection (VIP) scores >1.2). The obtained results shed new light on the allergic inflammatory response and may contribute to modification and improvement of the diagnostic and monitoring methods. Further, large-scale studies are still needed to explain mechanisms of action of studied compounds and to definitively prove their usefulness in clinical practice.
Kacper Packi; Joanna Matysiak; Eliza Matuszewska; Anna Bręborowicz; Zdzisława Kycler; Jan Matysiak. New Biomarkers of Hymenoptera Venom Allergy in a Group of Inflammation Factors. International Journal of Environmental Research and Public Health 2021, 18, 4011 .
AMA StyleKacper Packi, Joanna Matysiak, Eliza Matuszewska, Anna Bręborowicz, Zdzisława Kycler, Jan Matysiak. New Biomarkers of Hymenoptera Venom Allergy in a Group of Inflammation Factors. International Journal of Environmental Research and Public Health. 2021; 18 (8):4011.
Chicago/Turabian StyleKacper Packi; Joanna Matysiak; Eliza Matuszewska; Anna Bręborowicz; Zdzisława Kycler; Jan Matysiak. 2021. "New Biomarkers of Hymenoptera Venom Allergy in a Group of Inflammation Factors." International Journal of Environmental Research and Public Health 18, no. 8: 4011.
Venom immunotherapy (VIT) is administered to allergic patients to reduce the risk of dangerous systemic reactions following an insect sting. To better understand the mechanism of this treatment and its impact on the human organism, we analysed serum proteomic patterns obtained at five time-points from Hymenoptera-venom-allergic patients undergoing VIT. For statistical analyses, patients were additionally divided into two groups (high responders and low responders) according to serum sIgG4 levels. VIT was found to be associated with changes in seven proteins: the fibrinogen alpha chain, complement C4-A, complement C3, filamin-B, kininogen-1, myosin-9 and inter-alpha-trypsin inhibitor heavy chain H1. The number of discriminative m/z (mass-to-charge ratio) features increased up to the 90th day of VIT, which may be associated with the development of immunity after the administration of increased venom doses. It may also suggest that during VIT, there may occur processes involved not only in protein synthesis but also in protein degradation (caused by proteolytic venom components). The results are consistent with measured serum sIgG4 levels, which increased from 2.04 mgA/I at baseline to 7.25 mgA/I at 90 days. Moreover, the major proteomic changes were detected separately in the high responder group. This may suggest that changes in protein–peptide profiles reflect the actual response to VIT.
Joanna Matysiak; Eliza Matuszewska; Marek Kowalski; Sławomir Kosiński; Ewa Smorawska-Sabanty; Jan Matysiak. Association between Venom Immunotherapy and Changes in Serum Protein—Peptide Patterns. Vaccines 2021, 9, 249 .
AMA StyleJoanna Matysiak, Eliza Matuszewska, Marek Kowalski, Sławomir Kosiński, Ewa Smorawska-Sabanty, Jan Matysiak. Association between Venom Immunotherapy and Changes in Serum Protein—Peptide Patterns. Vaccines. 2021; 9 (3):249.
Chicago/Turabian StyleJoanna Matysiak; Eliza Matuszewska; Marek Kowalski; Sławomir Kosiński; Ewa Smorawska-Sabanty; Jan Matysiak. 2021. "Association between Venom Immunotherapy and Changes in Serum Protein—Peptide Patterns." Vaccines 9, no. 3: 249.
Asthma often begins in childhood, although making an early diagnosis is difficult. Clinical manifestations, the exclusion of other causes of bronchial obstruction, and responsiveness to anti-inflammatory therapy are the main tool of diagnosis. However, novel, precise, and functional biochemical markers are needed in the differentiation of asthma phenotypes, endotypes, and creating personalized therapy. The aim of the study was to search for metabolomic-based asthma biomarkers among free amino acids (AAs). A wide panel of serum-free AAs in asthmatic children, covering both proteinogenic and non-proteinogenic AAs, were analyzed. The examination included two groups of individuals between 3 and 18 years old: asthmatic children and the control group consisted of children with neither asthma nor allergies. High-performance liquid chromatography combined with tandem mass spectrometry (LC-MS/MS technique) was used for AA measurements. The data were analyzed by applying uni- and multivariate statistical tests. The obtained results indicate the decreased serum concentration of taurine, L-valine, DL-β-aminoisobutyric acid, and increased levels of ƴ-amino-n-butyric acid and L-arginine in asthmatic children when compared to controls. The altered concentration of these AAs can testify to their role in the pathogenesis of childhood asthma. The authors’ results should contribute to the future introduction of new diagnostic markers into clinical practice.
Joanna Matysiak; Agnieszka Klupczynska; Kacper Packi; Anna Mackowiak-Jakubowska; Anna Bręborowicz; Olga Pawlicka; Katarzyna Olejniczak; Zenon J. Kokot; Jan Matysiak. Alterations in Serum-Free Amino Acid Profiles in Childhood Asthma. International Journal of Environmental Research and Public Health 2020, 17, 4758 .
AMA StyleJoanna Matysiak, Agnieszka Klupczynska, Kacper Packi, Anna Mackowiak-Jakubowska, Anna Bręborowicz, Olga Pawlicka, Katarzyna Olejniczak, Zenon J. Kokot, Jan Matysiak. Alterations in Serum-Free Amino Acid Profiles in Childhood Asthma. International Journal of Environmental Research and Public Health. 2020; 17 (13):4758.
Chicago/Turabian StyleJoanna Matysiak; Agnieszka Klupczynska; Kacper Packi; Anna Mackowiak-Jakubowska; Anna Bręborowicz; Olga Pawlicka; Katarzyna Olejniczak; Zenon J. Kokot; Jan Matysiak. 2020. "Alterations in Serum-Free Amino Acid Profiles in Childhood Asthma." International Journal of Environmental Research and Public Health 17, no. 13: 4758.
Background Hymenoptera venom allergy is one of the most frequent causes of anaphylaxis. In its most severe form, the reaction to wasp and honey bee stings may be life-threatening. Therefore, immediate and proper diagnosis of venom allergy and implementation of suitable therapy are extremely important. Broadening the knowledge on the mechanism of the allergic reaction may contribute to the improvement of both diagnostic and treatment methods. Thus, this study aimed to discover changes in protein expression in serum of patients allergic to Hymenoptera (wasp and honeybee) venom and to point out proteins and peptides involved in the allergic inflammation. Methods Serum proteomic patterns typical to allergic patients and healthy volunteers were obtained with MALDI-TOF (matrix-assisted laser desorption/ionization-time of flight) mass spectrometer. The spectra were processed, analyzed and compared using advanced bioinformatics tools. The discriminative peaks were subjected to identification with liquid chromatography coupled with tandem mass spectrometry. Results This methodology allowed for the identification of four features differentiating between allergy and control groups. They were: fibrinogen alpha chain, coagulation factor XIII chain A, complement C4-A, and inter-alpha-trypsin inhibitor heavy chain H4. All of these proteins are involved in allergic inflammatory response. Conclusions Extending the knowledge of the Hymenoptera venom sensitization will contribute to the development of novel, sensitive and specific methods for quick and unambiguous allergy diagnosis. Understanding the basis of the allergy at the proteomic level will support the improvement of preventive and therapeutic measures.
Eliza Matuszewska; Joanna Matysiak; Anna Bręborowicz; Katarzyna Olejniczak; Zdzisława Kycler; Zenon Kokot; Jan Matysiak. Proteomic features characterization of Hymenoptera venom allergy. Allergy, Asthma & Clinical Immunology 2019, 15, 1 -8.
AMA StyleEliza Matuszewska, Joanna Matysiak, Anna Bręborowicz, Katarzyna Olejniczak, Zdzisława Kycler, Zenon Kokot, Jan Matysiak. Proteomic features characterization of Hymenoptera venom allergy. Allergy, Asthma & Clinical Immunology. 2019; 15 (1):1-8.
Chicago/Turabian StyleEliza Matuszewska; Joanna Matysiak; Anna Bręborowicz; Katarzyna Olejniczak; Zdzisława Kycler; Zenon Kokot; Jan Matysiak. 2019. "Proteomic features characterization of Hymenoptera venom allergy." Allergy, Asthma & Clinical Immunology 15, no. 1: 1-8.
Jan Matysiak; Joanna Matysiak; Anna Breborowicz; Zdzisława Kycler; Paweł Dereziński; Zenon J Kokot. Immune and clinical response to honeybee venom in beekeepers. Annals of Agricultural and Environmental Medicine 2016, 23, 120 -124.
AMA StyleJan Matysiak, Joanna Matysiak, Anna Breborowicz, Zdzisława Kycler, Paweł Dereziński, Zenon J Kokot. Immune and clinical response to honeybee venom in beekeepers. Annals of Agricultural and Environmental Medicine. 2016; 23 (1):120-124.
Chicago/Turabian StyleJan Matysiak; Joanna Matysiak; Anna Breborowicz; Zdzisława Kycler; Paweł Dereziński; Zenon J Kokot. 2016. "Immune and clinical response to honeybee venom in beekeepers." Annals of Agricultural and Environmental Medicine 23, no. 1: 120-124.
Introduction: Beekeepers are a group of people with high exposure to honeybee stings and with a very high risk of allergy to bee venom. Therefore, they are a proper population to study the correlations between clinical symptoms and results of diagnostic tests. Aim: The primary aim of...
Jan Matysiak; Joanna Matysiak; Anna Bręborowicz; Paweł Dereziński; Zenon Kokot. The correlation between anti phospholipase A 2 specific IgE and clinical symptoms after a bee sting in beekeepers. Advances in Dermatology and Allergology 2016, 3, 206 -210.
AMA StyleJan Matysiak, Joanna Matysiak, Anna Bręborowicz, Paweł Dereziński, Zenon Kokot. The correlation between anti phospholipase A 2 specific IgE and clinical symptoms after a bee sting in beekeepers. Advances in Dermatology and Allergology. 2016; 3 (3):206-210.
Chicago/Turabian StyleJan Matysiak; Joanna Matysiak; Anna Bręborowicz; Paweł Dereziński; Zenon Kokot. 2016. "The correlation between anti phospholipase A 2 specific IgE and clinical symptoms after a bee sting in beekeepers." Advances in Dermatology and Allergology 3, no. 3: 206-210.
The aim of this study was to explore the serum peptide profiles from honeybee stung and non-stung individuals. Two groups of serum samples obtained from 27 beekeepers were included in our study. The first group of samples was collected within 3 h after a bee sting (stung beekeepers), and the samples were collected from the same person a second time after at least six weeks after the last bee sting (non-stung beekeepers). Peptide profile spectra were determined using MALDI-TOF mass spectrometry combined with Omix, ZipTips and magnetic beads based on weak-cation exchange (MB-WCX) enrichment strategies in the mass range of 1–10 kDa. The samples were classified, and discriminative models were established by using the quick classifier, genetic algorithm and supervised neural network algorithms. All of the statistical algorithms used in this study allow distinguishing analyzed groups with high statistical significance, which confirms the influence of honeybee sting on the serum peptidome profile. The results of this study may broaden the understanding of the human organism’s response to honeybee venom. Due to the fact that our pilot study was carried out on relatively small datasets, it is necessary to conduct further proteomic research of the response to honeybee sting on a larger group of samples.
Jan Matysiak; Agata Światły; Joanna Hajduk; Joanna Matysiak; Zenon J. Kokot. Influence of Honeybee Sting on Peptidome Profile in Human Serum. Toxins 2015, 7, 1808 -1820.
AMA StyleJan Matysiak, Agata Światły, Joanna Hajduk, Joanna Matysiak, Zenon J. Kokot. Influence of Honeybee Sting on Peptidome Profile in Human Serum. Toxins. 2015; 7 (5):1808-1820.
Chicago/Turabian StyleJan Matysiak; Agata Światły; Joanna Hajduk; Joanna Matysiak; Zenon J. Kokot. 2015. "Influence of Honeybee Sting on Peptidome Profile in Human Serum." Toxins 7, no. 5: 1808-1820.
The aim of this study was to assess the response to a honeybee venom by analyzing serum levels of 34 free amino acids. Another goal of this study was to apply complex analytic-bioinformatic-clinical strategy based on up-to-date achievements of mass spectrometry in metabolomic profiling. The amino acid profiles were determined using hybrid triple quadrupole/linear ion trap mass spectrometer coupled with a liquid chromatography instrument. Serum samples were collected from 27 beekeepers within 3 hours after they were stung and after a minimum of 6 weeks following the last sting. The differences in amino acid profiles were evaluated using MetaboAnalyst and ROCCET web portals. Chemometric tests showed statistically significant differences in the levels of L-glutamine (Gln), L-glutamic acid (Glu), L-methionine (Met) and 3-methyl-L-histidine (3MHis) between the two analyzed groups of serum samples. Gln and Glu appeared to be the most important metabolites for distinguishing the beekeepers tested shortly after a bee sting from those tested at least 6 weeks later. The role of some amino acids in the response of an organism to the honeybee sting was also discussed. This study indicated that proposed methodology may allow to identify the individuals just after the sting and those who were stung at least 6 weeks earlier. The results we obtained will contribute to better understanding of the human body response to the honeybee sting.
Jan Matysiak; Paweł Dereziński; Agnieszka Klupczynska; Joanna Matysiak; Elzbieta Kaczmarek; Zenon Kokot. Effects of a Honeybee Sting on the Serum Free Amino Acid Profile in Humans. PLOS ONE 2014, 9, e103533 .
AMA StyleJan Matysiak, Paweł Dereziński, Agnieszka Klupczynska, Joanna Matysiak, Elzbieta Kaczmarek, Zenon Kokot. Effects of a Honeybee Sting on the Serum Free Amino Acid Profile in Humans. PLOS ONE. 2014; 9 (7):e103533.
Chicago/Turabian StyleJan Matysiak; Paweł Dereziński; Agnieszka Klupczynska; Joanna Matysiak; Elzbieta Kaczmarek; Zenon Kokot. 2014. "Effects of a Honeybee Sting on the Serum Free Amino Acid Profile in Humans." PLOS ONE 9, no. 7: e103533.
Jan Matysiak; Joanna Matysiak; Anna Bręborowicz; Zenon J Kokot. Diagnosis of hymenoptera venom allergy--with special emphasis on honeybee (Apis mellifera) venom allergy. Annals of Agricultural and Environmental Medicine 2013, 20, 1 .
AMA StyleJan Matysiak, Joanna Matysiak, Anna Bręborowicz, Zenon J Kokot. Diagnosis of hymenoptera venom allergy--with special emphasis on honeybee (Apis mellifera) venom allergy. Annals of Agricultural and Environmental Medicine. 2013; 20 (4):1.
Chicago/Turabian StyleJan Matysiak; Joanna Matysiak; Anna Bręborowicz; Zenon J Kokot. 2013. "Diagnosis of hymenoptera venom allergy--with special emphasis on honeybee (Apis mellifera) venom allergy." Annals of Agricultural and Environmental Medicine 20, no. 4: 1.