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Dr. Tatjana Ruskovska obtained her BSc (1990), MSc (1997), and Ph.D. (2002) degrees at the Faculty of Natural Sciences and Mathematics, Institute of Biology, Biochemistry and Physiology Group, Ss. Cyril and Methodius University, Skopje, North Macedonia. Positions held: biochemist and head of the laboratory at the Central Clinical Laboratory, Military Hospital in Skopje (1994 to 2010); joined the Faculty of Medical Sciences Goce Delcev University in Stip, North Macedonia (2010), where she was vice-dean from 2011 to 2014 and head of the school for doctoral studies (2018-2020). In the 2014/2015 academic year, she was appointed visiting professor in the Department of Biochemistry, Molecular Biology, and Biophysics at the University of Minnesota, USA, as a Fulbright Scholar. Currently, she works as a full professor of Clinical Chemistry and Biochemistry at the Faculty of Medical Sciences, Goce Delcev University in Stip, North Macedonia.
Various biomarkers of oxidative stress and redox status have been used in a number of clinical and epidemiological studies related to diseases and conditions that involve disturbances of the redox balance. However, a comprehensive study of diurnal variations of a set of biomarkers has not been conducted so far. Therefore, the aim of this study is to investigate circadian rhythm and time-of-day-effects of a set of frequently used biomarkers of oxidative stress, redox and antioxidant status in serum/plasma. These biomarkers include Reactive Oxygen Metabolites (ROM), Biological Antioxidant Potency (BAP), Total Thiols in Proteins (TTP), high-sensitive C-Reactive Protein (CRP) and Uric Acid (UA). During a 24-hr study, blood sampling was conducted 6 times at 4-hr intervals. The presence of circadian rhythm was analyzed with CircWave analysis, whereas the effect of time was analyzed with Repeated Measures ANOVA (RM-ANOVA). Thereby, the main focus was on the time points in working hours (8, 12 and 16 hr), which are used frequently in practice. Of all investigated biomarkers, only TTP in males demonstrated statistically significant circadian rhythm (p = 0.040). A statistically significant effect between all six time points with RM-ANOVA was observed for ROM, TTP and UA in both genders, and for BAP in females only. No statistically significant differences were observed between the time points 8 hr and 12 hr for any of the biomarkers that were assessed in our study. In conclusion, diurnal variations in some of the studied biomarkers that we demonstrate here should be taken into account when designing and conducting clinical and epidemiological studies. It is advised to standardize the time of sampling with a preference in the morning hours.
Tatjana Ruskovska; Piet Beekhof; Nevenka Velickova; Nikola Kamcev; Eugène Jansen. Circadian rhythm and time-of-day-effects of (anti)oxidant biomarkers for epidemiological studies. Free Radical Research 2021, 1 -7.
AMA StyleTatjana Ruskovska, Piet Beekhof, Nevenka Velickova, Nikola Kamcev, Eugène Jansen. Circadian rhythm and time-of-day-effects of (anti)oxidant biomarkers for epidemiological studies. Free Radical Research. 2021; ():1-7.
Chicago/Turabian StyleTatjana Ruskovska; Piet Beekhof; Nevenka Velickova; Nikola Kamcev; Eugène Jansen. 2021. "Circadian rhythm and time-of-day-effects of (anti)oxidant biomarkers for epidemiological studies." Free Radical Research , no. : 1-7.
Cardiometabolic disorders are among the leading causes of mortality in the human population. Dietary polyphenols exert beneficial effects on cardiometabolic health in humans. Molecular mechanisms, however, are not completely understood. Aiming to conduct in-depth integrative bioinformatic analyses to elucidate molecular mechanisms underlying the protective effects of polyphenols on cardiometabolic health, we first conducted a systematic literature search to identify human intervention studies with polyphenols that demonstrate improvement of cardiometabolic risk factors in parallel with significant nutrigenomic effects. Applying the predefined inclusion criteria, we identified 58 differentially expressed genes at mRNA level and 5 miRNAs, analyzed in peripheral blood cells with RT-PCR methods. Subsequent integrative bioinformatic analyses demonstrated that polyphenols modulate genes that are mainly involved in the processes such as inflammation, lipid metabolism, and endothelial function. We also identified 37 transcription factors that are involved in the regulation of polyphenol modulated genes, including RELA/NFKB1, STAT1, JUN, or SIRT1. Integrative bioinformatic analysis of mRNA and miRNA-target pathways demonstrated several common enriched pathways that include MAPK signaling pathway, TNF signaling pathway, PI3K-Akt signaling pathway, focal adhesion, or PPAR signaling pathway. These bioinformatic analyses represent a valuable source of information for the identification of molecular mechanisms underlying the beneficial health effects of polyphenols and potential target genes for future nutrigenetic studies.
Tatjana Ruskovska; Irena Budić-Leto; Karla Corral-Jara; Vladimir Ajdžanović; Anna Arola-Arnal; Francisca Bravo; Georgia-Eirini Deligiannidou; Jaroslav Havlik; Milkica Janeva; Elena Kistanova; Christos Kontogiorgis; Irena Krga; Marika Massaro; Marko Miler; Verica Milosevic; Christine Morand; Egeria Scoditti; Manuel Suárez; David Vauzour; Dragan Milenkovic. Systematic Bioinformatic Analyses of Nutrigenomic Modifications by Polyphenols Associated with Cardiometabolic Health in Humans—Evidence from Targeted Nutrigenomic Studies. Nutrients 2021, 13, 2326 .
AMA StyleTatjana Ruskovska, Irena Budić-Leto, Karla Corral-Jara, Vladimir Ajdžanović, Anna Arola-Arnal, Francisca Bravo, Georgia-Eirini Deligiannidou, Jaroslav Havlik, Milkica Janeva, Elena Kistanova, Christos Kontogiorgis, Irena Krga, Marika Massaro, Marko Miler, Verica Milosevic, Christine Morand, Egeria Scoditti, Manuel Suárez, David Vauzour, Dragan Milenkovic. Systematic Bioinformatic Analyses of Nutrigenomic Modifications by Polyphenols Associated with Cardiometabolic Health in Humans—Evidence from Targeted Nutrigenomic Studies. Nutrients. 2021; 13 (7):2326.
Chicago/Turabian StyleTatjana Ruskovska; Irena Budić-Leto; Karla Corral-Jara; Vladimir Ajdžanović; Anna Arola-Arnal; Francisca Bravo; Georgia-Eirini Deligiannidou; Jaroslav Havlik; Milkica Janeva; Elena Kistanova; Christos Kontogiorgis; Irena Krga; Marika Massaro; Marko Miler; Verica Milosevic; Christine Morand; Egeria Scoditti; Manuel Suárez; David Vauzour; Dragan Milenkovic. 2021. "Systematic Bioinformatic Analyses of Nutrigenomic Modifications by Polyphenols Associated with Cardiometabolic Health in Humans—Evidence from Targeted Nutrigenomic Studies." Nutrients 13, no. 7: 2326.
The Mediterranean diet (MD) has been sponsored worldwide as a healthy and sustainable diet. Our aim was to update and compare MD adherence and food choices across several Southern European countries: Spain (SP), Portugal (PT), Italy (IT), Greece (GR), and Cyprus (CY) (MED, Mediterranean), and Bulgaria (BG) and the Republic of North Macedonia (NMK) (non-MED, non-Mediterranean). Participants (N = 3145, ≥18 y) completed a survey (MeDiWeB) with sociodemographic, anthropometric, and food questions (14-item Mediterranean Diet Adherence Screener, 14-MEDAS). The MED and non-MED populations showed moderate (7.08 ± 1.96) and weak (5.58 ± 1.82) MD adherence, respectively, with significant yet small differences across countries (SP > PT > GR > IT > CY > BG > NMK, p-value < 0.001). The MED participants scored higher than the non-MED ones for most of the Mediterranean-typical foods, with the greatest differences found for olive oil (OO) and white meat preference. In most countries, ≥70% of the participants reported quantities of red meat, butter, sweet drinks, and desserts below the recommended cutoff points, whereas <50% achieved the targets for plant-based foods, OO, fish, and wine. Being a woman and increasing age were associated with superior adherence (p-value < 0.001), but differences were rather small. Our results suggest that the campaigns carried out to support and reinforce the MD and to promote plant-based foods have limited success across Southern Europe, and that more hard-hitting strategies are needed.
Stefano Quarta; Marika Massaro; Mihail Chervenkov; Teodora Ivanova; Dessislava Dimitrova; Rui Jorge; Vanda Andrade; Elena Philippou; Constantinos Zisimou; Viktorija Maksimova; Katarina Smilkov; Darinka Ackova; Lence Miloseva; Tatjana Ruskovska; Georgia Deligiannidou; Christos Kontogiorgis; Julio Sánchez-Meca; Paula Pinto; María-Teresa García-Conesa. Persistent Moderate-to-Weak Mediterranean Diet Adherence and Low Scoring for Plant-Based Foods across Several Southern European Countries: Are We Overlooking the Mediterranean Diet Recommendations? Nutrients 2021, 13, 1432 .
AMA StyleStefano Quarta, Marika Massaro, Mihail Chervenkov, Teodora Ivanova, Dessislava Dimitrova, Rui Jorge, Vanda Andrade, Elena Philippou, Constantinos Zisimou, Viktorija Maksimova, Katarina Smilkov, Darinka Ackova, Lence Miloseva, Tatjana Ruskovska, Georgia Deligiannidou, Christos Kontogiorgis, Julio Sánchez-Meca, Paula Pinto, María-Teresa García-Conesa. Persistent Moderate-to-Weak Mediterranean Diet Adherence and Low Scoring for Plant-Based Foods across Several Southern European Countries: Are We Overlooking the Mediterranean Diet Recommendations? Nutrients. 2021; 13 (5):1432.
Chicago/Turabian StyleStefano Quarta; Marika Massaro; Mihail Chervenkov; Teodora Ivanova; Dessislava Dimitrova; Rui Jorge; Vanda Andrade; Elena Philippou; Constantinos Zisimou; Viktorija Maksimova; Katarina Smilkov; Darinka Ackova; Lence Miloseva; Tatjana Ruskovska; Georgia Deligiannidou; Christos Kontogiorgis; Julio Sánchez-Meca; Paula Pinto; María-Teresa García-Conesa. 2021. "Persistent Moderate-to-Weak Mediterranean Diet Adherence and Low Scoring for Plant-Based Foods across Several Southern European Countries: Are We Overlooking the Mediterranean Diet Recommendations?" Nutrients 13, no. 5: 1432.
The Mediterranean diet (MD) and other lifestyle characteristics have been associated with well-being, a broad multiparameter concept that includes individual’s subjective assessment of their own well-being (SWB). Some studies have suggested that diet influences SWB, thus, this work aimed to add novel information on the association of MD and SWB in a sample of Portuguese adults. Data on sociodemographic, economic, lifestyle, diet, and SWB were collected through a self-filled online questionnaire. MD adherence was assessed by the Mediterranean Diet Adherence Screener (MEDAS) score ]. Results showed a moderate adherence to the MD in 490 Portuguese adults (mean MEDAS of 7.4 ± 2.1). A higher MD adherence was found to be significantly positively associated with women, employed individuals, a higher number of meals per day, and those with frequent contact with nature (p-value < 0.0025, using Bonferroni adjustment). As a novelty, this study divided the participants into low SWB, medium SWB, and medium to high SWB profiles (3.9 ± 1.0; 6.2 ± 1.0; 8.2 ± 1.3, respectively; p-value < 0.05), which reported significantly increasing MEDAS scores (6.5 ± 2.1; 7.3 ± 2.1; 7.8 ± 1.9; respectively, p-value < 0.05).
Vanda Andrade; Rui Jorge; María-Teresa García-Conesa; Elena Philippou; Marika Massaro; Mihail Chervenkov; Teodora Ivanova; Viktorija Maksimova; Katarina Smilkov; Darinka Gjorgieva Ackova; Lence Miloseva; Tatjana Ruskovska; Georgia Eirini Deligiannidou; Christos A. Kontogiorgis; Paula Pinto. Mediterranean Diet Adherence and Subjective Well-Being in a Sample of Portuguese Adults. Nutrients 2020, 12, 3837 .
AMA StyleVanda Andrade, Rui Jorge, María-Teresa García-Conesa, Elena Philippou, Marika Massaro, Mihail Chervenkov, Teodora Ivanova, Viktorija Maksimova, Katarina Smilkov, Darinka Gjorgieva Ackova, Lence Miloseva, Tatjana Ruskovska, Georgia Eirini Deligiannidou, Christos A. Kontogiorgis, Paula Pinto. Mediterranean Diet Adherence and Subjective Well-Being in a Sample of Portuguese Adults. Nutrients. 2020; 12 (12):3837.
Chicago/Turabian StyleVanda Andrade; Rui Jorge; María-Teresa García-Conesa; Elena Philippou; Marika Massaro; Mihail Chervenkov; Teodora Ivanova; Viktorija Maksimova; Katarina Smilkov; Darinka Gjorgieva Ackova; Lence Miloseva; Tatjana Ruskovska; Georgia Eirini Deligiannidou; Christos A. Kontogiorgis; Paula Pinto. 2020. "Mediterranean Diet Adherence and Subjective Well-Being in a Sample of Portuguese Adults." Nutrients 12, no. 12: 3837.
This study provides comprehensive validation of the 14-item Mediterranean Diet Adherence Screener (14-MEDAS) in an adult population from Greece (GR), Portugal (PT), Italy (IT), Spain (SP), Cyprus (CY), Republic of North Macedonia (NMK), and Bulgaria (BG). A moderate association between the 14-MEDAS and the reference food diary was estimated for the entire population (Pearson r = 0.573, p-value < 0.001; Intraclass Correlation Coefficient (ICC) = 0.692, p-value < 0.001) with the strongest correlation found in GR, followed by PT, IT, SP, and CY. These results were supported by kappa statistics in GR, PT, IT, and SP with ≥50% of food items exhibiting a fair or better agreement. Bland–Altman analyses showed an overestimation of the 14-MEDAS score in the whole population (0.79 ± 1.81, 95%Confidence Interval (CI) 0.61, 0.96), but this value was variable across countries, with GR, NMK, and BG exhibiting the lowest bias. Taking all analyses together, the validation achieved slightly better results in the Mediterranean countries but a definitive validation ranking order was not evident. Considering growing evidence of the shift from Mediterranean Diet (MD) adherence and of the importance of culture in making food choices it is crucial that we further improve validation protocols with specific applications to compare MD adherence across countries.
María-Teresa García-Conesa; Elena Philippou; Christos Pafilas; Marika Massaro; Stefano Quarta; Vanda Andrade; Rui Jorge; Mihail Chervenkov; Teodora Ivanova; Dessislava Dimitrova; Viktorija Maksimova; Katarina Smilkov; Darinka Gjorgieva Ackova; Lence Miloseva; Tatjana Ruskovska; Georgia Eirini Deligiannidou; Christos A. Kontogiorgis; Paula Pinto. Exploring the Validity of the 14-Item Mediterranean Diet Adherence Screener (MEDAS): A Cross-National Study in Seven European Countries around the Mediterranean Region. Nutrients 2020, 12, 2960 .
AMA StyleMaría-Teresa García-Conesa, Elena Philippou, Christos Pafilas, Marika Massaro, Stefano Quarta, Vanda Andrade, Rui Jorge, Mihail Chervenkov, Teodora Ivanova, Dessislava Dimitrova, Viktorija Maksimova, Katarina Smilkov, Darinka Gjorgieva Ackova, Lence Miloseva, Tatjana Ruskovska, Georgia Eirini Deligiannidou, Christos A. Kontogiorgis, Paula Pinto. Exploring the Validity of the 14-Item Mediterranean Diet Adherence Screener (MEDAS): A Cross-National Study in Seven European Countries around the Mediterranean Region. Nutrients. 2020; 12 (10):2960.
Chicago/Turabian StyleMaría-Teresa García-Conesa; Elena Philippou; Christos Pafilas; Marika Massaro; Stefano Quarta; Vanda Andrade; Rui Jorge; Mihail Chervenkov; Teodora Ivanova; Dessislava Dimitrova; Viktorija Maksimova; Katarina Smilkov; Darinka Gjorgieva Ackova; Lence Miloseva; Tatjana Ruskovska; Georgia Eirini Deligiannidou; Christos A. Kontogiorgis; Paula Pinto. 2020. "Exploring the Validity of the 14-Item Mediterranean Diet Adherence Screener (MEDAS): A Cross-National Study in Seven European Countries around the Mediterranean Region." Nutrients 12, no. 10: 2960.
Flavanol intake positively influences cardiometabolic health in humans – evidence fromin vitronutrigenomic studies.
Tatjana Ruskovska; Marika Massaro; Maria Annunziata Carluccio; Anna Arola-Arnal; Begoña Muguerza; Wim Vanden Berghe; Ken Declerck; Francisca Isabel Bravo; Nadia Calabriso; Emilie Combet; Eileen R. Gibney; Andreia Gomes; Marie-Paule Gonthier; Elena Kistanova; Irena Krga; Pedro Mena; Christine Morand; Claudia Nunes dos Santos; Sonia de Pascual-Teresa; Ana Rodriguez-Mateos; Egeria Scoditti; Manuel Suárez; Dragan Milenkovic. Systematic bioinformatic analysis of nutrigenomic data of flavanols in cell models of cardiometabolic disease. Food & Function 2020, 11, 5040 -5064.
AMA StyleTatjana Ruskovska, Marika Massaro, Maria Annunziata Carluccio, Anna Arola-Arnal, Begoña Muguerza, Wim Vanden Berghe, Ken Declerck, Francisca Isabel Bravo, Nadia Calabriso, Emilie Combet, Eileen R. Gibney, Andreia Gomes, Marie-Paule Gonthier, Elena Kistanova, Irena Krga, Pedro Mena, Christine Morand, Claudia Nunes dos Santos, Sonia de Pascual-Teresa, Ana Rodriguez-Mateos, Egeria Scoditti, Manuel Suárez, Dragan Milenkovic. Systematic bioinformatic analysis of nutrigenomic data of flavanols in cell models of cardiometabolic disease. Food & Function. 2020; 11 (6):5040-5064.
Chicago/Turabian StyleTatjana Ruskovska; Marika Massaro; Maria Annunziata Carluccio; Anna Arola-Arnal; Begoña Muguerza; Wim Vanden Berghe; Ken Declerck; Francisca Isabel Bravo; Nadia Calabriso; Emilie Combet; Eileen R. Gibney; Andreia Gomes; Marie-Paule Gonthier; Elena Kistanova; Irena Krga; Pedro Mena; Christine Morand; Claudia Nunes dos Santos; Sonia de Pascual-Teresa; Ana Rodriguez-Mateos; Egeria Scoditti; Manuel Suárez; Dragan Milenkovic. 2020. "Systematic bioinformatic analysis of nutrigenomic data of flavanols in cell models of cardiometabolic disease." Food & Function 11, no. 6: 5040-5064.
Oxidative damage of cells and tissues is broadly implicated in human pathophysiology, including cardiometabolic diseases. Polyphenols, as important constituents of the human diet and potentin vitrofree radical scavengers, have been extensively studied for their beneficial effects on cardiometabolic health. However, it has been demonstrated that thein vivoantioxidant activity of polyphenols is distinct from theirin vitrofree radical-scavenging capacity. Indeed, bioavailability of nutritional polyphenols is low and conditioned by complex mechanisms of absorption, distribution, metabolism and excretion. Nowadays, it is commonly accepted that the cellular antioxidant activity of polyphenols is mainly carried out via modification of transcription of genes involved in antioxidant defence. Importantly, polyphenols also contribute to cardiometabolic health by modulation of a plethora of cellular processes that are not directly associated with antioxidant enzymes, through nutri(epi)genomic mechanisms. Numerous human intervention studies have demonstrated beneficial effects of polyphenols on the key cardiometabolic risk factors. However, inconsistency of the results of some studies led to identification of the inter-individual variability in response to consumption of polyphenols. In perspective, a detailed investigation of the determinants of this inter-individual variability will potentially lead us towards personalised dietary recommendations. The phenomenon of inter-individual variability is also of relevance for supplementation with antioxidant (pro)vitamins.
T. Ruskovska; V. Maksimova; Dragan Milenkovic. Polyphenols in human nutrition: from thein vitroantioxidant capacity to the beneficial effects on cardiometabolic health and related inter-individual variability – an overview and perspective. British Journal of Nutrition 2019, 123, 241 -254.
AMA StyleT. Ruskovska, V. Maksimova, Dragan Milenkovic. Polyphenols in human nutrition: from thein vitroantioxidant capacity to the beneficial effects on cardiometabolic health and related inter-individual variability – an overview and perspective. British Journal of Nutrition. 2019; 123 (3):241-254.
Chicago/Turabian StyleT. Ruskovska; V. Maksimova; Dragan Milenkovic. 2019. "Polyphenols in human nutrition: from thein vitroantioxidant capacity to the beneficial effects on cardiometabolic health and related inter-individual variability – an overview and perspective." British Journal of Nutrition 123, no. 3: 241-254.
Evidence exists regarding the beneficial effects of diets rich in plant-based foods regarding the prevention of cardiometabolic diseases. These plant-based foods are an exclusive and abundant source of a variety of biologically active phytochemicals, including polyphenols, carotenoids, glucosinolates and phytosterols, with known health-promoting effects through a wide range of biological activities, such as improvements in endothelial function, platelet function, blood pressure, blood lipid profile and insulin sensitivity. We know that an individual’s physical/genetic makeup may influence their response to a dietary intervention, and thereby may influence the benefit/risk associated with consumption of a particular dietary constituent. This inter-individual variation in responsiveness has also been described for dietary plant bioactives but has not been explored in depth. To address this issue, the European scientific experts involved in the COST Action POSITIVe systematically analyzed data from published studies to assess the inter-individual variation in selected clinical biomarkers associated with cardiometabolic risk, in response to the consumption of plant-based bioactives (poly)phenols and phytosterols. The present review summarizes the main findings resulting from the meta-analyses already completed. Meta-analyses of randomized controlled trials conducted within POSITIVe suggest that age, sex, ethnicity, pathophysiological status and medication may be responsible for the heterogeneity in the biological responsiveness to (poly)phenol and phytosterol consumption and could lead to inconclusive results in some clinical trials aiming to demonstrate the health effects of specific dietary bioactive compounds. However, the contribution of these factors is not yet demonstrated consistently across all polyphenolic groups and cardiometabolic outcomes, partly due to the heterogeneity in trial designs, low granularity of data reporting, variety of food vectors and target populations, suggesting the need to implement more stringent reporting practices in the future studies. Studies investigating the effects of genetic background or gut microbiome on variability were limited and should be considered in future studies. Understanding why some bioactive plant compounds work effectively in some individuals but not, or less, in others is crucial for a full consideration of these compounds in future strategies of personalized nutrition for a better prevention of cardiometabolic disease. However, there is also still a need for the development of a substantial evidence-base to develop health strategies, food products or lifestyle solutions that embrace this variability.
Eileen R. Gibney; Dragan Milenkovic; Emilie Combet; Tatjana Ruskovska; Arno Greyling; Antonio González-Sarrías; Baujke De Roos; Francisco Tomás-Barberán; Christine Morand; Ana Rodriguez-Mateos. Factors influencing the cardiometabolic response to (poly)phenols and phytosterols: a review of the COST Action POSITIVe activities. European Journal of Nutrition 2019, 58, 37 -47.
AMA StyleEileen R. Gibney, Dragan Milenkovic, Emilie Combet, Tatjana Ruskovska, Arno Greyling, Antonio González-Sarrías, Baujke De Roos, Francisco Tomás-Barberán, Christine Morand, Ana Rodriguez-Mateos. Factors influencing the cardiometabolic response to (poly)phenols and phytosterols: a review of the COST Action POSITIVe activities. European Journal of Nutrition. 2019; 58 (2):37-47.
Chicago/Turabian StyleEileen R. Gibney; Dragan Milenkovic; Emilie Combet; Tatjana Ruskovska; Arno Greyling; Antonio González-Sarrías; Baujke De Roos; Francisco Tomás-Barberán; Christine Morand; Ana Rodriguez-Mateos. 2019. "Factors influencing the cardiometabolic response to (poly)phenols and phytosterols: a review of the COST Action POSITIVe activities." European Journal of Nutrition 58, no. 2: 37-47.
The European Cooperation in Science and Technology (COST) provides an ideal framework to establish multi-disciplinary research networks. COST Action BM1203 (EU-ROS) represents a consortium of researchers from different disciplines who are dedicated to providing new insights and tools for better understanding redox biology and medicine and, in the long run, to finding new therapeutic strategies to target dysregulated redox processes in various diseases. This report highlights the major achievements of EU-ROS as well as research updates and new perspectives arising from its members. The EU-ROS consortium comprised more than 140 active members who worked together for four years on the topics briefly described below. The formation of reactive oxygen and nitrogen species (RONS) is an established hallmark of our aerobic environment and metabolism but RONS also act as messengers via redox regulation of essential cellular processes. The fact that many diseases have been found to be associated with oxidative stress established the theory of oxidative stress as a trigger of diseases that can be corrected by antioxidant therapy. However, while experimental studies support this thesis, clinical studies still generate controversial results, due to complex pathophysiology of oxidative stress in humans. For future improvement of antioxidant therapy and better understanding of redox-associated disease progression detailed knowledge on the sources and targets of RONS formation and discrimination of their detrimental or beneficial roles is required. In order to advance this important area of biology and medicine, highly synergistic approaches combining a variety of diverse and contrasting disciplines are needed.
Javier Egea; Isabel Fabregat; Yves M. Frapart; Pietro Ghezzi; Agnes Görlach; Thomas Kietzmann; Kateryna Kubaichuk; Ulla G. Knaus; Manuela G. Lopez; Gloria Olaso González; Andreas Petry; Rainer Schulz; Jose Vina; Paul Winyard; Kahina Abbas; Opeyemi Stella Ademowo; Catarina B. Afonso; Ioanna Andreadou; Haike Antelmann; Fernando Antunes; Mutay Aslan; Markus Michael Bachschmid; Rui Barbosa; Vsevolod Belousov; Carsten Berndt; David Bernlohr; Esther Bertrán; Alberto Bindoli; Serge P. Bottari; Paula Brito; Guia Carrara; Ana I. Casas; Afroditi Chatzi; Niki Chondrogianni; Marcus Conrad; Marcus Cooke; João G. Costa; Antonio Cuadrado; Pham My-Chan Dang; Barbara De Smet; Bilge Debelec–Butuner; Irundika Dias; Joe Dan Dunn; Amanda Edson; Mariam El Assar; Jamel El-Benna; Péter Ferdinandy; Ana Fernandes; Kari E. Fladmark; Ulrich Förstermann; Rashid Giniatullin; Zoltán Giricz; Anikó Görbe; Helen Griffiths; Václav Hampl; Alina Hanf; Jan Herget; Pablo Hernansanz; Melanie Hillion; Jingjing Huang; Serap Ilikay; Pidder Jansen-Dürr; Vincent Jaquet; Jaap A. Joles; Balaraman Kalyanaraman; Danylo Kaminskyy; Mahsa Karbaschi; Marina Kleanthous; Lars-Oliver Klotz; Bato Korac; Kemal Sami Korkmaz; Rafal Koziel; Damir Kračun; Karl-Heinz Krause; Vladimír Křen; Thomas Krieg; João Laranjinha; Antigone Lazou; Huige Li; Antonio Martínez-Ruiz; Reiko Matsui; Gethin J. McBean; Stuart P. Meredith; Joris Messens; Veronica Miguel; Yuliya Mikhed; Irina Milisav; Lidija Milkovic; Antonio Miranda-Vizuete; Milos Mojovic; Maria Monsalve; Pierre-Alexis Mouthuy; John Mulvey; Thomas Münzel; Vladimir Muzykantov; Isabel T.N. Nguyen; Matthias Oelze; Nuno Oliveira; Carlos Palmeira; Nikoletta Papaevgeniou; Aleksandra Pavićević; Brandán Pedre; Fabienne Peyrot; Marios Phylactides; Gratiela G. Pircalabioru; Andrew R Pitt; Henrik Enghusen Poulsen; Ignacio Prieto; Maria Pia Rigobello; Natalia Robledinos-Antón; Esther Bertran Rodríguez; Anabela Rolo; Francis Rousset; Tatjana Ruskovska; Nuno Saraiva; Shlomo Sasson; Katrin Schröder; Khrystyna Semen; Tamara Seredenina; Anastasia Shakirzyanova; Geoffrey L. Smith; Thierry Soldati; Bebiana Sousa; Corinne Michelle Spickett; Ana Stancic; Marie José Stasia; Holger Steinbrenner; Višnja Stepanić; Sebastian Steven; Kostas Tokatlidis; Erkan Tuncay; Belma Turan; Fulvio Ursini; Jan Vacek; Olga Vajnerova; Kateřina Valentová; Frank Van Breusegem; Lokman Varisli; Elizabeth Veal; A. Suha Yalçın; Olha Yelisyeyeva; Neven Žarković; Martina Zatloukalová; Jacek Zielonka; Rhian M. Touyz; Andreas Papapetropoulos; Tilman Grune; Santiago Lamas; Harald H. H. W. Schmidt; Fabio Di Lisa; Andreas Daiber. European contribution to the study of ROS: A summary of the findings and prospects for the future from the COST action BM1203 (EU-ROS). Redox Biology 2017, 13, 94 -162.
AMA StyleJavier Egea, Isabel Fabregat, Yves M. Frapart, Pietro Ghezzi, Agnes Görlach, Thomas Kietzmann, Kateryna Kubaichuk, Ulla G. Knaus, Manuela G. Lopez, Gloria Olaso González, Andreas Petry, Rainer Schulz, Jose Vina, Paul Winyard, Kahina Abbas, Opeyemi Stella Ademowo, Catarina B. Afonso, Ioanna Andreadou, Haike Antelmann, Fernando Antunes, Mutay Aslan, Markus Michael Bachschmid, Rui Barbosa, Vsevolod Belousov, Carsten Berndt, David Bernlohr, Esther Bertrán, Alberto Bindoli, Serge P. Bottari, Paula Brito, Guia Carrara, Ana I. Casas, Afroditi Chatzi, Niki Chondrogianni, Marcus Conrad, Marcus Cooke, João G. Costa, Antonio Cuadrado, Pham My-Chan Dang, Barbara De Smet, Bilge Debelec–Butuner, Irundika Dias, Joe Dan Dunn, Amanda Edson, Mariam El Assar, Jamel El-Benna, Péter Ferdinandy, Ana Fernandes, Kari E. Fladmark, Ulrich Förstermann, Rashid Giniatullin, Zoltán Giricz, Anikó Görbe, Helen Griffiths, Václav Hampl, Alina Hanf, Jan Herget, Pablo Hernansanz, Melanie Hillion, Jingjing Huang, Serap Ilikay, Pidder Jansen-Dürr, Vincent Jaquet, Jaap A. Joles, Balaraman Kalyanaraman, Danylo Kaminskyy, Mahsa Karbaschi, Marina Kleanthous, Lars-Oliver Klotz, Bato Korac, Kemal Sami Korkmaz, Rafal Koziel, Damir Kračun, Karl-Heinz Krause, Vladimír Křen, Thomas Krieg, João Laranjinha, Antigone Lazou, Huige Li, Antonio Martínez-Ruiz, Reiko Matsui, Gethin J. McBean, Stuart P. Meredith, Joris Messens, Veronica Miguel, Yuliya Mikhed, Irina Milisav, Lidija Milkovic, Antonio Miranda-Vizuete, Milos Mojovic, Maria Monsalve, Pierre-Alexis Mouthuy, John Mulvey, Thomas Münzel, Vladimir Muzykantov, Isabel T.N. Nguyen, Matthias Oelze, Nuno Oliveira, Carlos Palmeira, Nikoletta Papaevgeniou, Aleksandra Pavićević, Brandán Pedre, Fabienne Peyrot, Marios Phylactides, Gratiela G. Pircalabioru, Andrew R Pitt, Henrik Enghusen Poulsen, Ignacio Prieto, Maria Pia Rigobello, Natalia Robledinos-Antón, Esther Bertran Rodríguez, Anabela Rolo, Francis Rousset, Tatjana Ruskovska, Nuno Saraiva, Shlomo Sasson, Katrin Schröder, Khrystyna Semen, Tamara Seredenina, Anastasia Shakirzyanova, Geoffrey L. Smith, Thierry Soldati, Bebiana Sousa, Corinne Michelle Spickett, Ana Stancic, Marie José Stasia, Holger Steinbrenner, Višnja Stepanić, Sebastian Steven, Kostas Tokatlidis, Erkan Tuncay, Belma Turan, Fulvio Ursini, Jan Vacek, Olga Vajnerova, Kateřina Valentová, Frank Van Breusegem, Lokman Varisli, Elizabeth Veal, A. Suha Yalçın, Olha Yelisyeyeva, Neven Žarković, Martina Zatloukalová, Jacek Zielonka, Rhian M. Touyz, Andreas Papapetropoulos, Tilman Grune, Santiago Lamas, Harald H. H. W. Schmidt, Fabio Di Lisa, Andreas Daiber. European contribution to the study of ROS: A summary of the findings and prospects for the future from the COST action BM1203 (EU-ROS). Redox Biology. 2017; 13 ():94-162.
Chicago/Turabian StyleJavier Egea; Isabel Fabregat; Yves M. Frapart; Pietro Ghezzi; Agnes Görlach; Thomas Kietzmann; Kateryna Kubaichuk; Ulla G. Knaus; Manuela G. Lopez; Gloria Olaso González; Andreas Petry; Rainer Schulz; Jose Vina; Paul Winyard; Kahina Abbas; Opeyemi Stella Ademowo; Catarina B. Afonso; Ioanna Andreadou; Haike Antelmann; Fernando Antunes; Mutay Aslan; Markus Michael Bachschmid; Rui Barbosa; Vsevolod Belousov; Carsten Berndt; David Bernlohr; Esther Bertrán; Alberto Bindoli; Serge P. Bottari; Paula Brito; Guia Carrara; Ana I. Casas; Afroditi Chatzi; Niki Chondrogianni; Marcus Conrad; Marcus Cooke; João G. Costa; Antonio Cuadrado; Pham My-Chan Dang; Barbara De Smet; Bilge Debelec–Butuner; Irundika Dias; Joe Dan Dunn; Amanda Edson; Mariam El Assar; Jamel El-Benna; Péter Ferdinandy; Ana Fernandes; Kari E. Fladmark; Ulrich Förstermann; Rashid Giniatullin; Zoltán Giricz; Anikó Görbe; Helen Griffiths; Václav Hampl; Alina Hanf; Jan Herget; Pablo Hernansanz; Melanie Hillion; Jingjing Huang; Serap Ilikay; Pidder Jansen-Dürr; Vincent Jaquet; Jaap A. Joles; Balaraman Kalyanaraman; Danylo Kaminskyy; Mahsa Karbaschi; Marina Kleanthous; Lars-Oliver Klotz; Bato Korac; Kemal Sami Korkmaz; Rafal Koziel; Damir Kračun; Karl-Heinz Krause; Vladimír Křen; Thomas Krieg; João Laranjinha; Antigone Lazou; Huige Li; Antonio Martínez-Ruiz; Reiko Matsui; Gethin J. McBean; Stuart P. Meredith; Joris Messens; Veronica Miguel; Yuliya Mikhed; Irina Milisav; Lidija Milkovic; Antonio Miranda-Vizuete; Milos Mojovic; Maria Monsalve; Pierre-Alexis Mouthuy; John Mulvey; Thomas Münzel; Vladimir Muzykantov; Isabel T.N. Nguyen; Matthias Oelze; Nuno Oliveira; Carlos Palmeira; Nikoletta Papaevgeniou; Aleksandra Pavićević; Brandán Pedre; Fabienne Peyrot; Marios Phylactides; Gratiela G. Pircalabioru; Andrew R Pitt; Henrik Enghusen Poulsen; Ignacio Prieto; Maria Pia Rigobello; Natalia Robledinos-Antón; Esther Bertran Rodríguez; Anabela Rolo; Francis Rousset; Tatjana Ruskovska; Nuno Saraiva; Shlomo Sasson; Katrin Schröder; Khrystyna Semen; Tamara Seredenina; Anastasia Shakirzyanova; Geoffrey L. Smith; Thierry Soldati; Bebiana Sousa; Corinne Michelle Spickett; Ana Stancic; Marie José Stasia; Holger Steinbrenner; Višnja Stepanić; Sebastian Steven; Kostas Tokatlidis; Erkan Tuncay; Belma Turan; Fulvio Ursini; Jan Vacek; Olga Vajnerova; Kateřina Valentová; Frank Van Breusegem; Lokman Varisli; Elizabeth Veal; A. Suha Yalçın; Olha Yelisyeyeva; Neven Žarković; Martina Zatloukalová; Jacek Zielonka; Rhian M. Touyz; Andreas Papapetropoulos; Tilman Grune; Santiago Lamas; Harald H. H. W. Schmidt; Fabio Di Lisa; Andreas Daiber. 2017. "European contribution to the study of ROS: A summary of the findings and prospects for the future from the COST action BM1203 (EU-ROS)." Redox Biology 13, no. : 94-162.
Background: Disturbed oxidant/antioxidant status is involved in pathogenesis of anemia in end stage renal disease. There is evidence that vitamin E supplementation can increase blood hemoglobin in chronically hemodialyzed patients. However, the interindividual variation in response to the supplementation has not been fully addressed. Methods: 24 chronically hemodialyzed patients were supplemented with vitamin E (400 IU/day) in a period of two months. They had already been treated with erythropoiesis stimulating agents (ESA) and iron on a long-term basis, which was continued during the study period. A group of 20 healthy volunteers served as control subjects. Complete blood count, general biochemistry assays, the redox status by total thiols, oxidative stress by reactive oxygen metabolites, antioxidant status by biological antioxidant potential, and vitamin E (α- and γ- tocopherol) were measured before the start of supplementation, one month and two months later. Results: Overall, the vitamin E supplementation did not cause an increase of blood hemoglobin, hematocrit or red blood cells. However, 50 % of the patients with basal blood hemoglobin below 12.0 g/dL (N = 10) responded to the supplementation with its continuous increase. In addition, vitamin E exhibited a slight prooxidant effect only in the subgroup of patients with basal blood hemoglobin of ≥ 12.0 g/dL, two months after the start of supplementation (decreased total thiols: 300 ± 31 vs. 277 ± 36 µmol/L, p < 0.05; increased reactive oxygen metabolites: 183 ± 140 vs. 287 ± 112 CARR U, p > 0.05; decreased biological antioxidant potential: 2278 ± 150 vs. 2171 ± 126 µEq/L, p < 0.025), which coincided with their significantly increased serum α-tocopherol concentrations in comparison to the patients with basal blood hemoglobin below 12.0 g/dL (41.3 ± 7.2 vs. 59.9 ± 19.2 µmol/L, p < 0.025). Conclusions: When treated with ESA and iron on a long-term basis, the response to the vitamin E supplementation in chronically hemodialyzed patients is largely dependent on their basal blood hemoglobin and serum vitamin E concentrations.
Tatjana Ruskovska; Ankica Pop-Kostova; Eugene Hjm Jansen; Risto Antarorov; Icko Gjorgoski. Vitamin E supplementation in chronically hemodialyzed patients – influence on blood hemoglobin and plasma (anti)oxidant status. International Journal for Vitamin and Nutrition Research 2017, 87, 139 -148.
AMA StyleTatjana Ruskovska, Ankica Pop-Kostova, Eugene Hjm Jansen, Risto Antarorov, Icko Gjorgoski. Vitamin E supplementation in chronically hemodialyzed patients – influence on blood hemoglobin and plasma (anti)oxidant status. International Journal for Vitamin and Nutrition Research. 2017; 87 (3-4):139-148.
Chicago/Turabian StyleTatjana Ruskovska; Ankica Pop-Kostova; Eugene Hjm Jansen; Risto Antarorov; Icko Gjorgoski. 2017. "Vitamin E supplementation in chronically hemodialyzed patients – influence on blood hemoglobin and plasma (anti)oxidant status." International Journal for Vitamin and Nutrition Research 87, no. 3-4: 139-148.
Several epidemiological studies have linked flavonols with decreased risk of cardiovascular disease (CVD). However, some heterogeneity in the individual physiological responses to the consumption of these compounds has been identified. This meta‐analysis aimed to study the effect of flavonol supplementation on biomarkers of CVD risk such as, blood lipids, blood pressure and plasma glucose, as well as factors affecting their inter‐individual variability. Data from 18 human randomized controlled trials were pooled and the effect was estimated using fixed or random effects meta‐analysis model and reported as difference in means (DM). Variability in the response of blood lipids to supplementation with flavonols was assessed by stratifying various population subgroups: age, sex, country, and health status. Results showed significant reductions in total cholesterol (DM = −0.10 mmol/L; 95% CI: −0.20, −0.01), LDL cholesterol (DM = −0.14 mmol/L; Nutrients 2017, 9, 117 2 of 21 95% CI: −0.21, 0.07), and triacylglycerol (DM = −0.10 mmol/L; 95% CI: −0.18, 0.03), and a significant increase in HDL cholesterol (DM = 0.05 mmol/L; 95% CI: 0.02, 0.07). A significant reduction was also observed in fasting plasma glucose (DM = −0.18 mmol/L; 95%CI: −0.29, −0.08), and in blood pressure (SBP: DM = −4.84 mmHg; 95% CI: −5.64, −4.04; DBP: DM = −3.32 mmHg; 95% CI: -4.09, -2.55). Subgroup analysis showed a more pronounced effect of flavonol intake in participants from Asian countries and in participants with diagnosed disease or dyslipidemia, compared to healthy and normal baseline values. In conclusion, flavonol consumption improved biomarkers of CVD risk, however, country of origin and health status may influence the effect of flavonol intake on blood lipid levels
Regina Menezes; Ana Rodriguez‐Mateos; Antonia Kaltsatou; Antonio González‐Sarrías; Arno Greyling; Christoforos Giannaki; Cristina Andres‐Lacueva; Dragan Milenkovic; Eileen R. Gibney; Eileen R Gibney Julie Dumont; Manuel Schär; Mar Garcia‐Aloy; Susana Alejandra Palma‐Duran; Tatjana Ruskovska; Viktorija Maksimova; Emilie Combet; Paula Pinto. Impact of Flavonols on Cardiometabolic Biomarkers: A Meta‐Analysis of Randomized Controlled Human Trials to Explore the Role of Inter‐Individual Variability. Nutrients 2017, 9, 117 .
AMA StyleRegina Menezes, Ana Rodriguez‐Mateos, Antonia Kaltsatou, Antonio González‐Sarrías, Arno Greyling, Christoforos Giannaki, Cristina Andres‐Lacueva, Dragan Milenkovic, Eileen R. Gibney, Eileen R Gibney Julie Dumont, Manuel Schär, Mar Garcia‐Aloy, Susana Alejandra Palma‐Duran, Tatjana Ruskovska, Viktorija Maksimova, Emilie Combet, Paula Pinto. Impact of Flavonols on Cardiometabolic Biomarkers: A Meta‐Analysis of Randomized Controlled Human Trials to Explore the Role of Inter‐Individual Variability. Nutrients. 2017; 9 (2):117.
Chicago/Turabian StyleRegina Menezes; Ana Rodriguez‐Mateos; Antonia Kaltsatou; Antonio González‐Sarrías; Arno Greyling; Christoforos Giannaki; Cristina Andres‐Lacueva; Dragan Milenkovic; Eileen R. Gibney; Eileen R Gibney Julie Dumont; Manuel Schär; Mar Garcia‐Aloy; Susana Alejandra Palma‐Duran; Tatjana Ruskovska; Viktorija Maksimova; Emilie Combet; Paula Pinto. 2017. "Impact of Flavonols on Cardiometabolic Biomarkers: A Meta‐Analysis of Randomized Controlled Human Trials to Explore the Role of Inter‐Individual Variability." Nutrients 9, no. 2: 117.
AIM: To determine whether cigarette smoking, as a risk factor for CAD, affects oxidative stress.MATERIAL AND METHODS: The study included patients with CAD divided according to smoking status and number of cigarettes smoked during a whole day. In all subjects were examined biological markers of oxidative stress (concentration of oxidants and activity of antioxidant enzymes).RESULTS: The study included 300 patients with CAD, with an average age of 62.97 ± 11.18 years, with a predominance of males. Of the total, 34.0% were active smokers, and 43.0% were non-smokers. The number of the most active smokers smoked cigarettes 1-20/day. In terms of concentration of oxidants (MDA and HP) it has not proved a significant difference between smokers versus non-smokers. Over the activity of antioxidant enzymes (SOD, CAT and GPX) statistically significant difference was found in the activity of GPX and among active smokers with CAD and non-smokers with CAD (p = 0.039).CONCLUSION:Smoking as a risk factor for CAD is closely associated with increased oxidative stress and the number of cigarettes smoked plays an important role in increasing the level of oxidative damage and reduced antioxidant defence.AIM: To determine whether cigarette smoking, as a risk factor for CAD, affects (anti)oxidant status.MATERIAL AND METHODS: The study included patients with CAD, divided according to their smoking status and the number of cigarettes smoked during a day. Biological markers of oxidative stress (concentration of oxidants and activity of antioxidant enzymes) were measured in all subjects.RESULTS: The study included 300 patients with CAD, (average age of 63 ± 11 years), predominantly males. Of the total, 34.0% were active smokers, 23.0% were former smokers, and 43.0% were non-smokers. Most of the active smokers smoked 1-20 cigarettes/day. In terms of concentration of oxidants (MDA and HP) there was not a significant difference between smokers versus non-smokers. As for the activity of antioxidant enzymes (SOD, CAT and GPX), a statistically significant difference was found in the activity of GPX among the active smokers with CAD and the non-smokers with CAD (p = 0.039). CONCLUSION: Smoking as a risk factor for CAD is closely associated with increased oxidative stress, and the number of cigarettes smoked plays an important role in increasing the level of oxidative damage and reducing antioxidant defence.
Gordana Kamceva; Zorica Arsova-Sarafinovska; Tatjana Ruskovska; Milka Zdravkovska; Lidija Kamceva-Panova; Elisaveta Stikova. Cigarette Smoking and Oxidative Stress in Patients with Coronary Artery Disease. Open Access Macedonian Journal of Medical Sciences 2016, 4, 636 -640.
AMA StyleGordana Kamceva, Zorica Arsova-Sarafinovska, Tatjana Ruskovska, Milka Zdravkovska, Lidija Kamceva-Panova, Elisaveta Stikova. Cigarette Smoking and Oxidative Stress in Patients with Coronary Artery Disease. Open Access Macedonian Journal of Medical Sciences. 2016; 4 (4):636-640.
Chicago/Turabian StyleGordana Kamceva; Zorica Arsova-Sarafinovska; Tatjana Ruskovska; Milka Zdravkovska; Lidija Kamceva-Panova; Elisaveta Stikova. 2016. "Cigarette Smoking and Oxidative Stress in Patients with Coronary Artery Disease." Open Access Macedonian Journal of Medical Sciences 4, no. 4: 636-640.
In this review, we disclose a selection of serum/plasma biomarkers of (anti)oxidant status related to nutrition, which can be used for measurements in large-scale epidemiological studies. From personal experience, we have come to the following proposal of a set of biomarkers for nutritional intake, (anti)oxidant status, and redox status. We have selected the individual antioxidant vitamins E and A, and the carotenoids which can be measured in large series by HPLC. In addition, vitamin C was selected, which can be measured by an auto-analyzer or HPLC. As a biomarker for oxidative stress, the ROM assay (reactive oxygen metabolites) was selected; for the redox status, the total thiol assay; and for the total antioxidant status the BAP assay (biological antioxidant potential). All of these biomarkers can be measured in large quantities by an auto-analyzer. Critical points in biomarker validation with respect to blood sampling, storage conditions, and measurements are discussed. With the selected biomarkers, a good set is presented for use in the risk assessment between nutrition and (chronic) diseases in large-scale epidemiological studies. Examples of the successful application of these biomarkers in large international studies are presented.
Eugène Jansen; Tatjana Ruskovska. Serum Biomarkers of (Anti)Oxidant Status for Epidemiological Studies. International Journal of Molecular Sciences 2015, 16, 27378 -27390.
AMA StyleEugène Jansen, Tatjana Ruskovska. Serum Biomarkers of (Anti)Oxidant Status for Epidemiological Studies. International Journal of Molecular Sciences. 2015; 16 (11):27378-27390.
Chicago/Turabian StyleEugène Jansen; Tatjana Ruskovska. 2015. "Serum Biomarkers of (Anti)Oxidant Status for Epidemiological Studies." International Journal of Molecular Sciences 16, no. 11: 27378-27390.
Significance: Oxidative stress is considered to be an important component of various diseases. A vast number of methods have been developed and used in virtually all diseases to measure the extent and nature of oxidative stress, ranging from oxidation of DNA to proteins, lipids, and free amino acids. Recent Advances: An increased understanding of the biology behind diseases and redox biology has led to more specific and sensitive tools to measure oxidative stress markers, which are very diverse and sometimes very low in abundance. Critical Issues: The literature is very heterogeneous. It is often difficult to draw general conclusions on the significance of oxidative stress biomarkers, as only in a limited proportion of diseases have a range of different biomarkers been used, and different biomarkers have been used to study different diseases. In addition, biomarkers are often measured using nonspecific methods, while specific methodologies are often too sophisticated or laborious for routine clinical use. Future Directions: Several markers of oxidative stress still represent a viable biomarker opportunity for clinical use. However, positive findings with currently used biomarkers still need to be validated in larger sample sizes and compared with current clinical standards to establish them as clinical diagnostics. It is important to realize that oxidative stress is a nuanced phenomenon that is difficult to characterize, and one biomarker is not necessarily better than others. The vast diversity in oxidative stress between diseases and conditions has to be taken into account when selecting the most appropriate biomarker. Antioxid. Redox Signal. 23, 1144–1170.
Jeroen Frijhoff; Paul Winyard; Neven Zarkovic; Sean Davies; Roland Stocker; David Cheng; Annie R Knight; Emma Louise Taylor; Jeannette Oettrich; Tatjana Ruskovska; Ana Cipak Gasparovic; Antonio Cuadrado; Daniela Weber; Henrik Enghusen Poulsen; Tilman Grune; Harald H. H. W. Schmidt; Pietro Ghezzi. Clinical Relevance of Biomarkers of Oxidative Stress. Antioxidants & Redox Signaling 2015, 23, 1144 -1170.
AMA StyleJeroen Frijhoff, Paul Winyard, Neven Zarkovic, Sean Davies, Roland Stocker, David Cheng, Annie R Knight, Emma Louise Taylor, Jeannette Oettrich, Tatjana Ruskovska, Ana Cipak Gasparovic, Antonio Cuadrado, Daniela Weber, Henrik Enghusen Poulsen, Tilman Grune, Harald H. H. W. Schmidt, Pietro Ghezzi. Clinical Relevance of Biomarkers of Oxidative Stress. Antioxidants & Redox Signaling. 2015; 23 (14):1144-1170.
Chicago/Turabian StyleJeroen Frijhoff; Paul Winyard; Neven Zarkovic; Sean Davies; Roland Stocker; David Cheng; Annie R Knight; Emma Louise Taylor; Jeannette Oettrich; Tatjana Ruskovska; Ana Cipak Gasparovic; Antonio Cuadrado; Daniela Weber; Henrik Enghusen Poulsen; Tilman Grune; Harald H. H. W. Schmidt; Pietro Ghezzi. 2015. "Clinical Relevance of Biomarkers of Oxidative Stress." Antioxidants & Redox Signaling 23, no. 14: 1144-1170.
Many molecular epidemiology studies focusing on high prevalent diseases, such as metabolic disorders and cancer, investigate metabolic and hormonal markers. In general, sampling for these markers can occur at any time-point during the day or after an overnight fast. However, environmental factors, such as light exposure and food intake might affect the levels of these markers, since they provide input for the internal time-keeping system. When diurnal variation is larger than the inter-individual variation, time of day should be taken into account. Importantly, heterogeneity in diurnal variation and disturbance of circadian rhythms among a study population might increasingly occur as a result of our increasing 24/7 economy and related variation in exposure to environmental factors (such as light and food). The aim of the present study was to determine whether a set of often used biomarkers shows diurnal variation in a setting resembling large molecular epidemiology studies, i.e., non-fasted and limited control possibilities for other environmental influences. We show that markers for which diurnal variation is not an issue are adrenocorticotropic hormone, follicle stimulating hormone, estradiol and high-density lipoprotein. For all other tested markers diurnal variation was observed in at least one gender (cholesterol, cortisol, dehydroepiandrosterone sulfate, free fatty acids, low-density lipoprotein, luteinizing hormone, prolactin, progesterone, testosterone, triglycerides, total triiodothyronine and thyroid-stimulating hormone) or could not reliably be detected (human growth hormone). Thus, studies investigating these markers should take diurnal variation into account, for which we provide some options. Furthermore, our study indicates the need for investigating diurnal variation (in literature or experimentally) before setting up studies measuring markers in routine and controlled settings, especially since time-of-day likely matters for many more markers than the ones investigated in the present study.
Linda W. M. Van Kerkhof; Kirsten C. G. Van Dycke; Eugene H. J. M. Jansen; Piet K. Beekhof; Conny T. M. Van Oostrom; Tatjana Ruskovska; Nevenka Velickova; Nikola Kamcev; Jeroen L.A. Pennings; Harry Van Steeg; Wendy Rodenburg. Diurnal Variation of Hormonal and Lipid Biomarkers in a Molecular Epidemiology-Like Setting. PLoS ONE 2015, 10, e0135652 .
AMA StyleLinda W. M. Van Kerkhof, Kirsten C. G. Van Dycke, Eugene H. J. M. Jansen, Piet K. Beekhof, Conny T. M. Van Oostrom, Tatjana Ruskovska, Nevenka Velickova, Nikola Kamcev, Jeroen L.A. Pennings, Harry Van Steeg, Wendy Rodenburg. Diurnal Variation of Hormonal and Lipid Biomarkers in a Molecular Epidemiology-Like Setting. PLoS ONE. 2015; 10 (8):e0135652.
Chicago/Turabian StyleLinda W. M. Van Kerkhof; Kirsten C. G. Van Dycke; Eugene H. J. M. Jansen; Piet K. Beekhof; Conny T. M. Van Oostrom; Tatjana Ruskovska; Nevenka Velickova; Nikola Kamcev; Jeroen L.A. Pennings; Harry Van Steeg; Wendy Rodenburg. 2015. "Diurnal Variation of Hormonal and Lipid Biomarkers in a Molecular Epidemiology-Like Setting." PLoS ONE 10, no. 8: e0135652.
The aim of this study was to evaluate the genotoxicity of ionizing radiation on medical personnel using the micronucleus assay and to determine the human health risk. Paired Student?s t-test shows significant statistical difference between the total number of binucleated (BN) cells with micronuclei within the two groups (exposed and control) (t=6,812; p
Nevenka Velickova; Misko Milev; Tatjana Ruskovska; Biljana Petrova; Bojana Nedeljkovik; Pale Gorgieva. Cytogenetic abnormalities in lymphocytes evaluated with micronucleus assay in medical personnel occupationally exposed to ionizing radiation. Genetika 2015, 47, 927 -939.
AMA StyleNevenka Velickova, Misko Milev, Tatjana Ruskovska, Biljana Petrova, Bojana Nedeljkovik, Pale Gorgieva. Cytogenetic abnormalities in lymphocytes evaluated with micronucleus assay in medical personnel occupationally exposed to ionizing radiation. Genetika. 2015; 47 (3):927-939.
Chicago/Turabian StyleNevenka Velickova; Misko Milev; Tatjana Ruskovska; Biljana Petrova; Bojana Nedeljkovik; Pale Gorgieva. 2015. "Cytogenetic abnormalities in lymphocytes evaluated with micronucleus assay in medical personnel occupationally exposed to ionizing radiation." Genetika 47, no. 3: 927-939.
Chronically haemodialysed end-stage renal disease patients are at high risk of morbidity arising from complications of dialysis, the underlying pathology that has led to renal disease and the complex pathology of chronic kidney disease. Anaemia is commonplace and its origins are multifactorial, involving reduced renal erythropoietin production, accumulation of uremic toxins and an increase in erythrocyte fragility. Oxidative damage is a common risk factor in renal disease and its co-morbidities and is known to cause erythrocyte fragility. Therefore, we have investigated the hypothesis that specific erythrocyte membrane proteins are more oxidised in end-stage renal disease patients and that vitamin C supplementation can ameliorate membrane protein oxidation. Eleven patients and 15 control subjects were recruited to the study. Patients were supplemented with 2 × 500 mg vitamin C per day for 4 weeks. Erythrocyte membrane proteins were prepared pre- and post-vitamin C supplementation for determination of protein oxidation. Total protein carbonyls were reduced by vitamin C supplementation but not by dialysis when investigated by enzyme linked immunosorbent assay. Using a western blot to detect oxidised proteins, one protein band, later identified as containing ankyrin, was found to be oxidised in patients but not controls and was reduced significantly by 60% in all patients after dialysis and by 20% after vitamin C treatment pre-dialysis. Ankyrin oxidation analysis may be useful in a stratified medicines approach as a possible marker to identify requirements for intervention in dialysis patients.
Tatjana Ruskovska; Stuart J Bennett; C. R. Brown; Slave Dimitrov; Nikola Kamcev; H. R. Griffiths. Ankyrin is the major oxidised protein in erythrocyte membranes from end-stage renal disease patients on chronic haemodialysis and oxidation is decreased by dialysis and vitamin C supplementation. Free Radical Research 2014, 49, 175 -185.
AMA StyleTatjana Ruskovska, Stuart J Bennett, C. R. Brown, Slave Dimitrov, Nikola Kamcev, H. R. Griffiths. Ankyrin is the major oxidised protein in erythrocyte membranes from end-stage renal disease patients on chronic haemodialysis and oxidation is decreased by dialysis and vitamin C supplementation. Free Radical Research. 2014; 49 (2):175-185.
Chicago/Turabian StyleTatjana Ruskovska; Stuart J Bennett; C. R. Brown; Slave Dimitrov; Nikola Kamcev; H. R. Griffiths. 2014. "Ankyrin is the major oxidised protein in erythrocyte membranes from end-stage renal disease patients on chronic haemodialysis and oxidation is decreased by dialysis and vitamin C supplementation." Free Radical Research 49, no. 2: 175-185.
Background. Various biomarkers and assays have been used for assessment of (anti)oxidant status in hemodialysis patients, including those intended for measurement of serum total (anti)oxidants, most often as a part of panel biomarkers.Methods. Serum (anti)oxidant status was measured in 32 chronically hemodialyzed patients and in 47 healthy persons, using two oxidations and three antioxidant assays.Results. The patients before the hemodialysis session have had higher values of total oxidants in comparison to the healthy persons, with a further increase during the hemodialysis. These findings were confirmed with both oxidation assays, but they differ in the percentage of increase and the statistical significance. All three antioxidant assays showed significantly higher values of the total serum antioxidants in the patients before the hemodialysis session in comparison to the healthy persons, and their significant decrease during the hemodialysis. However, the assays differ in the percentage of decrease, its statistical significance, and the correlations with uric acid.Conclusion. The variability of results of total (anti)oxidants which are obtained using different assays should be taken into account when interpreting data from clinical studies of oxidative stress, especially in complex pathologies such as chronic hemodialysis.
Tatjana Ruskovska; Eugene H. J. M. Jansen; Risto Antarorov. Evaluation of Assays for Measurement of Serum (Anti)oxidants in Hemodialysis Patients. BioMed Research International 2014, 2014, 1 -8.
AMA StyleTatjana Ruskovska, Eugene H. J. M. Jansen, Risto Antarorov. Evaluation of Assays for Measurement of Serum (Anti)oxidants in Hemodialysis Patients. BioMed Research International. 2014; 2014 ():1-8.
Chicago/Turabian StyleTatjana Ruskovska; Eugene H. J. M. Jansen; Risto Antarorov. 2014. "Evaluation of Assays for Measurement of Serum (Anti)oxidants in Hemodialysis Patients." BioMed Research International 2014, no. : 1-8.
The present study aimed to analyze the biological effects induced by bioaccumulation of metals in common bean (Phaseolus vulgaris L.). Effects of mineral nutrient imbalance, total antioxidants level and DNA damage induced by accumulation of heavy metals, were investigated in bean seedlings treated with two selected metal concentrations for 7 days. Metal content is analyzed by inductively coupled plasma – atomic emission spectrometer (ICP-AES), for total antioxidants level assessment the Ferric-Reducing Antioxidant Power (FRAP) assay is used and Random Amplified Polymorphic DNA (RAPD) method was applied for investigation of DNA damages. The increasing metal concentration in the treatment medium changed synchronously metal content in samples, and decreased total antioxidant activity in all samples with exception only for samples treated with Ni and Cd. The obtained “DNA fingerprints” demonstrated that the increasing metal concentrations induced changes in RAPD profiles (disappearance and/or appearance of bands in comparison with untreated control samples). The highest number of missing bands was observed in samples treated with zinc (total 4 bands) and nickel (total 4 bands) at both concentrations. These results suggested that mineral nutrient imbalance is involved in changes of antioxidant levels and DNA damages of the seedlings, which may help to understand the mechanism of metal toxicity in plants.
Darinka Gjorgieva; Tatjana Kadifkova Panovska; Tatjana Ruskovska; Katerina Bačeva; Trajce Stafilov. Mineral nutrient imbalance, total antioxidants level and DNA damage in common bean (Phaseolus vulgaris L.) exposed to heavy metals. Physiology and Molecular Biology of Plants 2013, 19, 499 -507.
AMA StyleDarinka Gjorgieva, Tatjana Kadifkova Panovska, Tatjana Ruskovska, Katerina Bačeva, Trajce Stafilov. Mineral nutrient imbalance, total antioxidants level and DNA damage in common bean (Phaseolus vulgaris L.) exposed to heavy metals. Physiology and Molecular Biology of Plants. 2013; 19 (4):499-507.
Chicago/Turabian StyleDarinka Gjorgieva; Tatjana Kadifkova Panovska; Tatjana Ruskovska; Katerina Bačeva; Trajce Stafilov. 2013. "Mineral nutrient imbalance, total antioxidants level and DNA damage in common bean (Phaseolus vulgaris L.) exposed to heavy metals." Physiology and Molecular Biology of Plants 19, no. 4: 499-507.
Heavy metals have the potential to interact and induce several stress responses in the plants; thus, effects of heavy metal stress on DNA damages and total antioxidants level inUrtica dioicaleaves and stems were investigated. The samples are sampled from areas with different metal exposition. Metal content was analyzed by Inductively Coupled Plasma-Atomic Emission Spectrometer (ICP-AES), for total antioxidants level assessment the Ferric-Reducing Antioxidant Power (FRAP) assay was used, and genomic DNA isolation from frozen plant samples was performed to obtain DNA fingerprints of investigated plant. It was found that heavy metal contents in stems generally changed synchronously with those in leaves of the plant, and extraneous metals led to imbalance of mineral nutrient elements. DNA damages were investigated by Random Amplified Polymorphic DNA (RAPD) technique, and the results demonstrated that the samples exposed to metals yielded a large number of new fragments (total 12) in comparison with the control sample. This study showed that DNA stability is highly affected by metal pollution which was identified by RAPD markers. Results suggested that heavy metal stress influences antioxidant status and also induces DNA damages inU. dioicawhich may help to understand the mechanisms of metals genotoxicity.
Darinka Gjorgieva; Tatjana Kadifkova Panovska; Tatjana Ruskovska; Katerina Bačeva; Trajče Stafilov. Influence of Heavy Metal Stress on Antioxidant Status and DNA Damage inUrtica dioica. BioMed Research International 2013, 2013, 1 -6.
AMA StyleDarinka Gjorgieva, Tatjana Kadifkova Panovska, Tatjana Ruskovska, Katerina Bačeva, Trajče Stafilov. Influence of Heavy Metal Stress on Antioxidant Status and DNA Damage inUrtica dioica. BioMed Research International. 2013; 2013 ():1-6.
Chicago/Turabian StyleDarinka Gjorgieva; Tatjana Kadifkova Panovska; Tatjana Ruskovska; Katerina Bačeva; Trajče Stafilov. 2013. "Influence of Heavy Metal Stress on Antioxidant Status and DNA Damage inUrtica dioica." BioMed Research International 2013, no. : 1-6.