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The aim of this study was to conduct a first evaluation on the co-occurrence of aflatoxins (AF) M1, B1, B2, G1 and G2; fumonisins (F) B1 and B2; deoxynivalenol (DON); de-epoxydeoxinivalenol (DOM-1); ochratoxin A (OTA); zearalenone (ZEN); α-zearalenol (α-ZEL); and β-zearalenol (β-ZEL) in 68 samples of fluid milk consumed in Pirassununga, São Paulo, Brazil. The probable daily intake (PDI) was also calculated for each mycotoxin evaluated. Mycotoxins were determined by liquid chromatography coupled to mass spectrometry. Sixty-two (91.2%) samples contained at least one type of mycotoxin. AFM1 was found in 6 samples (8.8%), and none of them presented concentrations above the Brazilian maximum permitted level in milk (500 ng/L). Low levels of non-regulated mycotoxins DOM-1, OTA, FB1, FB2, α-ZEL and β-ZEL were found in 6 (8.8%), 17 (25%), 10 (14.7%), 3 (4.4%), 39 (57.4%) and 28 (41.2%) samples of milk, respectively. None of the PDIs calculated for the quantified mycotoxins were above recommended values, indicating low exposure through milk consumption in the area studied. However, 21 samples (30.9%) contained 2–4 types of mycotoxins, which warrants concern about the potential adverse effects of mycotoxin mixtures in milks.
Matheus Frey; Roice Rosim; Carlos Oliveira. Mycotoxin Co-Occurrence in Milks and Exposure Estimation: A Pilot Study in São Paulo, Brazil. Toxins 2021, 13, 507 .
AMA StyleMatheus Frey, Roice Rosim, Carlos Oliveira. Mycotoxin Co-Occurrence in Milks and Exposure Estimation: A Pilot Study in São Paulo, Brazil. Toxins. 2021; 13 (8):507.
Chicago/Turabian StyleMatheus Frey; Roice Rosim; Carlos Oliveira. 2021. "Mycotoxin Co-Occurrence in Milks and Exposure Estimation: A Pilot Study in São Paulo, Brazil." Toxins 13, no. 8: 507.
In this study, the changes in oncogenic and tumor suppressor signaling pathways in liver and their association with serum and urinary biomarkers of aflatoxin exposure were evaluated in Wistar rats fed diets containing aflatoxin B1 (AFB1) for 90 days. Rats were divided into four groups (n = 15 per group) and assigned to dietary treatments containing 0 (control), 50 (AFB50), 100 (AFB100) and 200 μg AFB1 kg−1 diet (AFB200). Multiple preneoplastic foci of hepatocytes marked with glutathione-S-transferase-placental form (GST-P) were identified in AFB100 and AFB200 groups. Hepatocellular damage induced by AFB1 resulted in overexpression of cyclin D1 and β-catenin. The liver expression of retinoblastoma (Rb) and p27Kip1 decreased in AFB100 and AFB200 groups, confirming the favorable conditions for neoplastic progression to hepatocellular carcinoma. All samples from rats fed AFB1-contaminated diets had quantifiable AFB1-lysine in serum or urinary AFM1 and AFB1-N7-guanine, with mean levels of 20.42–50.34 ng mL−1, 5.31–37.68 and 39.15–126.37 ng mg−1 creatinine, respectively. Positive correlations were found between AFB1-lysine, AFM1 or AFB1-N7-guanine and GST-P+, β-catenin+ and cyclin D1+ hepatocytes, while Rb + cells negatively correlated with those AFB1 exposure biomarkers. The pathways evaluated are critical molecular mechanisms of AFB1-induced hepatocarcinogenesis in rats.
Letícia A. Apolinário; Leandra N.Z. Ramalho; Motahareh Hashemi Moosavi; Alessandra V. Jager; Marlei J. Augusto; Maurício R. Trotta; Tânia Petta; Amin Mousavi Khaneghah; Carlos A.F. Oliveira; Fernando S. Ramalho. Oncogenic and tumor suppressor pathways in subchronic aflatoxicosis in rats: Association with serum and urinary aflatoxin exposure biomarkers. Food and Chemical Toxicology 2021, 153, 112263 .
AMA StyleLetícia A. Apolinário, Leandra N.Z. Ramalho, Motahareh Hashemi Moosavi, Alessandra V. Jager, Marlei J. Augusto, Maurício R. Trotta, Tânia Petta, Amin Mousavi Khaneghah, Carlos A.F. Oliveira, Fernando S. Ramalho. Oncogenic and tumor suppressor pathways in subchronic aflatoxicosis in rats: Association with serum and urinary aflatoxin exposure biomarkers. Food and Chemical Toxicology. 2021; 153 ():112263.
Chicago/Turabian StyleLetícia A. Apolinário; Leandra N.Z. Ramalho; Motahareh Hashemi Moosavi; Alessandra V. Jager; Marlei J. Augusto; Maurício R. Trotta; Tânia Petta; Amin Mousavi Khaneghah; Carlos A.F. Oliveira; Fernando S. Ramalho. 2021. "Oncogenic and tumor suppressor pathways in subchronic aflatoxicosis in rats: Association with serum and urinary aflatoxin exposure biomarkers." Food and Chemical Toxicology 153, no. : 112263.
This study aimed to evaluate the in vitro ability of heat-killed (HK) and acid-killed (AK) cells from three commercial strains of lactic acid bacteria, alone or in combination with sorbitan monostearate (SM), to bind to aflatoxin M1 (AFM1) in skimmed milk at concentrations of 0.05, 0.2 and 0.5 ng mL−1. The stability of the AFM1-bacterial cell bonds and the cell wall and exopolysaccharides (EPS) contribution to AFM1 decontamination were also investigated. Compared with HK cells, AK cells exhibited higher binding capacities in milk containing the highest AFM1 level, with maximum removal of 81.4, 56.8, and 50.8% by Lactococcus lactis ssp. cremoris, Lacticaseibacillus rhamnosus (formerly Lactobacillus rhamnosus), and L. lactis ssp. lactis at 1010 cells mL−1, respectively. The combination of HK cells with SM increased the AFM1 binding capacity, although no effect was found regarding AK cells. SM treatment also enhanced the stability of the AFM1 bond formed with both HK and AK cells. Cell wall isolates and EPS were the major components involved in the AFM1 binding process. Combining SM with HK cells is an interesting approach to reduce AFM1 levels in milk, although further studies are needed to optimize the adsorbing process.
Khurram Muaz; Muhammad Riaz; Roice Eliana Rosim; Saeed Akhtar; Carlos Humberto Corassin; Bruna Leonel Gonçalves; Carlos Augusto Fernandes Oliveira. In vitro ability of nonviable cells of lactic acid bacteria strains in combination with sorbitan monostearate to bind to aflatoxin M1 in skimmed milk. LWT - Food Science and Technology 2021, 147, 111666 .
AMA StyleKhurram Muaz, Muhammad Riaz, Roice Eliana Rosim, Saeed Akhtar, Carlos Humberto Corassin, Bruna Leonel Gonçalves, Carlos Augusto Fernandes Oliveira. In vitro ability of nonviable cells of lactic acid bacteria strains in combination with sorbitan monostearate to bind to aflatoxin M1 in skimmed milk. LWT - Food Science and Technology. 2021; 147 ():111666.
Chicago/Turabian StyleKhurram Muaz; Muhammad Riaz; Roice Eliana Rosim; Saeed Akhtar; Carlos Humberto Corassin; Bruna Leonel Gonçalves; Carlos Augusto Fernandes Oliveira. 2021. "In vitro ability of nonviable cells of lactic acid bacteria strains in combination with sorbitan monostearate to bind to aflatoxin M1 in skimmed milk." LWT - Food Science and Technology 147, no. : 111666.
The effect of cold atmospheric pressure plasma (CAPP) on three strains of Staphylococcus aureus and one strain of Listeria monocytogenes on gel discs was studied. A plasma jet operating in argon was used and exposure times were from 5 to 120 s. Bacteria had been isolated from dairy environments. In comparison, peracetic acid (PAA, 0.5% v/v) applied on cell suspensions at the same treatment times was studied. The cell viability was measured by flow cytometry and the culturable cells were determined by plate count technique. PAA reduced nearly 7 log cycles of both microorganisms after a short time (10 s). Concurrently, membrane permeabilization, RNA and DNA damage, and lack of esterase activity could be demonstrated. CAPP showed a mild biocidal effect with a maximum reduction of 1–2 log cycle after 120 s, and no significant impact on membrane permeabilization, RNA and DNA damage, or esterase activity. Therefore, for the selected processing parameters, PAA remains as more effective treatment in terms of inactivating pathogenic bacteria in dairy environments, reaching ~7 log cycles reductions. Further studies should determine the effect of higher CAPP exposure times on S. aureus and L. monocytogenes strains.
Sarah H.I. Lee; Antje Fröhling; Oliver Schlüter; Carlos H. Corassin; Elaine C.P. De Martinis; Virginia F. Alves; Tatiana C. Pimentel; Carlos A.F. Oliveira. Cold atmospheric pressure plasma inactivation of dairy associated planktonic cells of Listeria monocytogenes and Staphylococcus aureus. LWT 2021, 146, 111452 .
AMA StyleSarah H.I. Lee, Antje Fröhling, Oliver Schlüter, Carlos H. Corassin, Elaine C.P. De Martinis, Virginia F. Alves, Tatiana C. Pimentel, Carlos A.F. Oliveira. Cold atmospheric pressure plasma inactivation of dairy associated planktonic cells of Listeria monocytogenes and Staphylococcus aureus. LWT. 2021; 146 ():111452.
Chicago/Turabian StyleSarah H.I. Lee; Antje Fröhling; Oliver Schlüter; Carlos H. Corassin; Elaine C.P. De Martinis; Virginia F. Alves; Tatiana C. Pimentel; Carlos A.F. Oliveira. 2021. "Cold atmospheric pressure plasma inactivation of dairy associated planktonic cells of Listeria monocytogenes and Staphylococcus aureus." LWT 146, no. : 111452.
The initial stages of human life including the fetal period, infancy and early childhood are the most critical periods of human growth and development. Due to immature immune system and rapid development phase, this is the most sensitive phase to health challenges. The exposure to xenobiotics of high toxicity, such as aflatoxins (AFs), can interfere with normal development of the body and potentially lead to severe health complications. This article provides a comprehensive review on the occurrence and health implications of AFs exposure in the early stages of life, including the impacts on child growth, development, immune and hepatic systems. The mechanisms involved in AFs’ toxicity and control measures in infant foods are also described, along with a discussion on recent intervention strategies to mitigate the early life exposure to dietary AFs. Human AFs exposure via utero and through breast milk, infant formula milk and infant foods has been linked to a number of health implications including adverse birth outcomes, impaired growth and development, suppressed immune system, and hepatic dysfunction, especially in African and Asian countries. The occurrence of AFs in infant foods in developing countries, even when reasonably strict regulations are in place, indicate that much needs to be done to ensure the proper implementation of the regulations, in order to achieve significant reductions in exposure. This carries with it many economic and cultural challenges that need to be addressed, including implementation of good agricultural practices and intervention programs aiming to supplement nutrients to reduce child malnutrition and decrease the consumption of aflatoxin-contaminated foods.
Amir Ismail; Iqra Naeem; Yun Yun Gong; Michael N. Routledge; Saeed Akhtar; Muhammad Riaz; Leandra Naira Zambelli Ramalho; Carlos Augusto Fernandes de Oliveira; Zubair Ismail. Early life exposure to dietary aflatoxins, health impact and control perspectives: A review. Trends in Food Science & Technology 2021, 112, 212 -224.
AMA StyleAmir Ismail, Iqra Naeem, Yun Yun Gong, Michael N. Routledge, Saeed Akhtar, Muhammad Riaz, Leandra Naira Zambelli Ramalho, Carlos Augusto Fernandes de Oliveira, Zubair Ismail. Early life exposure to dietary aflatoxins, health impact and control perspectives: A review. Trends in Food Science & Technology. 2021; 112 ():212-224.
Chicago/Turabian StyleAmir Ismail; Iqra Naeem; Yun Yun Gong; Michael N. Routledge; Saeed Akhtar; Muhammad Riaz; Leandra Naira Zambelli Ramalho; Carlos Augusto Fernandes de Oliveira; Zubair Ismail. 2021. "Early life exposure to dietary aflatoxins, health impact and control perspectives: A review." Trends in Food Science & Technology 112, no. : 212-224.
In this study, the occurrence of aflatoxins (AFs), fumonisins (FBs), ochratoxin A (OTA), deoxynivalenol (DON), zearalenone (ZEN) and some of their metabolites were assessed in breast milk and urine of lactating women (N = 74) from Pirassununga, São Paulo, Brazil. Exposure estimations through urinary mycotoxin biomarkers was also performed. Samples were collected in four sampling times (May and August 2018, February and July 2019) and analyzed by liquid chromatography coupled to tandem mass spectrometry. Aflatoxin M1 (AFM1) was not detected in breast milk. However, two samples (3%) presented FB1 at 2200 and 3400 ng/L, while 4 samples (5%) had OTA at the median level of 360 ng/L. In urine, AFM1 and aflatoxin P1 (AFP1) were found in 51 and 11% of samples, respectively (median levels: 0.16 and 0.07 ng/mg creatinine, respectively). Urinary DON (median level: 38.59 ng/mg creatinine), OTA (median level: 2.38 ng/mg creatinine) and ZEN (median level: 0.02 ng/mg of creatinine) were quantified in 18, 8 and 10% of the samples, respectively. Mean probable daily intake (PDI) values based on urinary biomarkers were 1.58, 1.09, 5.07, and 0.05 μg/kg body weight/day for AFM1, DON, OTA, and ZEN, respectively. Although a low mycotoxin occurrence was detected in breast milk, the PDI for the genotoxic AFs was much higher than those reported previously in Brazil, while PDI values obtained for OTA and DON were higher than recommended tolerable daily intakes. These outcomes warrant concern on the exposure of lactating women to these mycotoxins in the studied area.
Carolina F.S.C. Coppa; Amanda C. Cirelli; Bruna L. Gonçalves; Eliana M.B. Barnabé; Tânia Petta; Larissa T. Franco; Fardin Javanmardi; Amin Mousavi Khaneghah; Sarah H.I. Lee; Carlos H. Corassin; Carlos A.F. Oliveira. Mycotoxin occurrence in breast milk and exposure estimation of lactating mothers using urinary biomarkers in São Paulo, Brazil. Environmental Pollution 2021, 279, 116938 .
AMA StyleCarolina F.S.C. Coppa, Amanda C. Cirelli, Bruna L. Gonçalves, Eliana M.B. Barnabé, Tânia Petta, Larissa T. Franco, Fardin Javanmardi, Amin Mousavi Khaneghah, Sarah H.I. Lee, Carlos H. Corassin, Carlos A.F. Oliveira. Mycotoxin occurrence in breast milk and exposure estimation of lactating mothers using urinary biomarkers in São Paulo, Brazil. Environmental Pollution. 2021; 279 ():116938.
Chicago/Turabian StyleCarolina F.S.C. Coppa; Amanda C. Cirelli; Bruna L. Gonçalves; Eliana M.B. Barnabé; Tânia Petta; Larissa T. Franco; Fardin Javanmardi; Amin Mousavi Khaneghah; Sarah H.I. Lee; Carlos H. Corassin; Carlos A.F. Oliveira. 2021. "Mycotoxin occurrence in breast milk and exposure estimation of lactating mothers using urinary biomarkers in São Paulo, Brazil." Environmental Pollution 279, no. : 116938.
Ceylon cinnamon is a possible reserve of proanthocyanidins. This study aimed at the extraction and characterization of an extract rich in proanthocyanidins from Ceylon cinnamon. Extraction kinetics data were determined using hydroalcoholic solution as the solvent, different temperatures, and solid:solvent mass ratios up to 60 min. The extract obtained under the best conditions was analyzed by high‐performance liquid chromatography/diode array detection, and the main fractions collected were evaluated by mass spectrometry and antioxidant capacity assays. Increases in temperature and solid:solvent ratio led to an increase in the effective diffusivity coefficient and proanthocyanidins extraction yield. The temperature of 60°C and solid:solvent ratio of 1:7.5 during 30 min of extraction provided quick extraction of these compounds with a maximum yield of 99%. The main fractions collected were identified as being cinnamaldehyde, cinnamic acid, and oligomeric proanthocyanidins which yielded the highest antioxidant capacity. Therefore, this extract becomes potential for use as a functional ingredient. Novelty impact statement A rich proanthocyanidins extract of Ceylon cinnamon was obtained. Higher values of temperature and solid:solvent ratio improve the extraction. The extract contains mainly proanthocyanidins, cinnamic acid and cinnamaldehyde. Among the fractions, phenolics are the main antioxidant compounds. The obtained extract has potential to be used as a functional ingredient.
Volnei Brito de Souza; Augusto Tasch Holkem; Marcelo Thomazini; Tânia Petta; Fabricio Luiz Tulini; Carlos Augusto Fernandes de Oliveira; Maria Inés Genovese; Christianne Elisabete Da Costa Rodrigues; Carmen Sílvia Fávaro Trindade. Study of extraction kinetics and characterization of proanthocyanidin‐rich extract from Ceylon cinnamon ( Cinnamomum zeylanicum ). Journal of Food Processing and Preservation 2021, 45, e15429 .
AMA StyleVolnei Brito de Souza, Augusto Tasch Holkem, Marcelo Thomazini, Tânia Petta, Fabricio Luiz Tulini, Carlos Augusto Fernandes de Oliveira, Maria Inés Genovese, Christianne Elisabete Da Costa Rodrigues, Carmen Sílvia Fávaro Trindade. Study of extraction kinetics and characterization of proanthocyanidin‐rich extract from Ceylon cinnamon ( Cinnamomum zeylanicum ). Journal of Food Processing and Preservation. 2021; 45 (5):e15429.
Chicago/Turabian StyleVolnei Brito de Souza; Augusto Tasch Holkem; Marcelo Thomazini; Tânia Petta; Fabricio Luiz Tulini; Carlos Augusto Fernandes de Oliveira; Maria Inés Genovese; Christianne Elisabete Da Costa Rodrigues; Carmen Sílvia Fávaro Trindade. 2021. "Study of extraction kinetics and characterization of proanthocyanidin‐rich extract from Ceylon cinnamon ( Cinnamomum zeylanicum )." Journal of Food Processing and Preservation 45, no. 5: e15429.
Aflatoxin M1 (AFM1) was determined in milk and Minas Frescal cheese (MFC) manufactured in dairy plants from São Paulo, Brazil. The average AFM1 levels in raw, pasteurised milk and MFC were 0.028 ± 0.009 µg, 0.023 ± 0.006 µg/L and 0.113 ± 0.092 µg/kg, respectively. AFM1 in raw (n = 1; 4%) and pasteurised (n = 2; 8%) milks exceeded the European maximum permitted level (MPL) of 0.05 µg/L. AFM1 concentration rate for MFC was 4.9 ± 1.2. Although AFM1 in all MFC samples was below the Brazilian’s MPL for cheese (2.5 µg/kg), the levels found may increase the overall exposure of consumers to dietary aflatoxins.
Bruna L Gonçalves; Rômulo D Ulliana; Gustavo L P A Ramos; Adriano G Cruz; Carlos A F Oliveira; Eliana S Kamimura; Carlos H Corassin. Occurrence of aflatoxin M 1 in milk and Minas Frescal cheese manufactured in Brazilian dairy plants. International Journal of Dairy Technology 2021, 74, 431 -434.
AMA StyleBruna L Gonçalves, Rômulo D Ulliana, Gustavo L P A Ramos, Adriano G Cruz, Carlos A F Oliveira, Eliana S Kamimura, Carlos H Corassin. Occurrence of aflatoxin M 1 in milk and Minas Frescal cheese manufactured in Brazilian dairy plants. International Journal of Dairy Technology. 2021; 74 (2):431-434.
Chicago/Turabian StyleBruna L Gonçalves; Rômulo D Ulliana; Gustavo L P A Ramos; Adriano G Cruz; Carlos A F Oliveira; Eliana S Kamimura; Carlos H Corassin. 2021. "Occurrence of aflatoxin M 1 in milk and Minas Frescal cheese manufactured in Brazilian dairy plants." International Journal of Dairy Technology 74, no. 2: 431-434.
In this trial, 60 samples of raw cow’s milk collected during summer and winter in five collective centers from Khorasan Razavi Province of Iran were analyzed for penicillin residues, somatic cell counts (SCC), mesophilic counts (TMC) and adulteration materials. The mean penicillin level (0.57 ± 0.20 μg/L) was much lower than the maximum permitted level (MPL) recommended by the Codex Alimentarius Commission (4 μg/L). However, the mean SCC and TMC (Log 5.71 ± 0.08 cells/mL and Log 6.18 ± 0.11 colony forming units (CFU)/mL, respectively) exceeded their respective MPLs adopted in Iran for grade C milk (Log 5.69 cells/mL and Log 6.0 CFU/mL, respectively), with higher counts (P < 0.05) in the summer. High frequencies of samples containing salt (53-60%), detergents (16-20%) and formalin (7-10%) were observed in milk samples, stressing the need for stringent control measures to improve the quality of milk delivered in the evaluated collection centers.
Marzieh Hajmohammadi; Reza Valizadeh; Mohammad Norouzi Ebdalabadi; Abassali Naserian; Carlos Augusto Fernandes de Oliveira. Seasonal variations in some quality parameters of milk produced in Khorasan Razavi Province, Iran. Food Science and Technology 2021, 1 .
AMA StyleMarzieh Hajmohammadi, Reza Valizadeh, Mohammad Norouzi Ebdalabadi, Abassali Naserian, Carlos Augusto Fernandes de Oliveira. Seasonal variations in some quality parameters of milk produced in Khorasan Razavi Province, Iran. Food Science and Technology. 2021; ():1.
Chicago/Turabian StyleMarzieh Hajmohammadi; Reza Valizadeh; Mohammad Norouzi Ebdalabadi; Abassali Naserian; Carlos Augusto Fernandes de Oliveira. 2021. "Seasonal variations in some quality parameters of milk produced in Khorasan Razavi Province, Iran." Food Science and Technology , no. : 1.
The aim of this study was to evaluate the capacity of two strains of lactic acid bacteria (LAB), Lactobacillus rhamnosus and Lactococcus lactis, and a yeast strain, Saccharomyces cerevisiae, inactivated by heat (121 °C, 10 min), from binding to aflatoxin M1 (AFM1), as well as the interaction between these microorganisms, aflatoxin M1 and the Minas Frescal cheese matrix after 2 and 30 days of storage. The ability of LABs and S. cerevisiae to bind AFM1 to Minas Frescal cheese was evaluated by high performance liquid chromatography (HPLC) composed of a fluorescence detector. The interaction between these microorganisms and AFM1 was evaluated using a scanning electron microscope composed of a backscattered electron detector with a voltage of 15 kV and magnifications of 1000 ×, 5000 × and 8000 ×. The use of microorganisms as a biological method is efficient in reducing AFM1 in Minas Frescal cheese and does not affect the microbiological parameters. AFM1 reduction varied according to the microorganism used in the treatments. S cerevisiae showed greater capacity to bind AFM1 over time, compared to LABs. Scanning electron microscopy was especially useful, confirming that lactic acid bacteria and S. cerevisae were able to bind AFM1 particles in Minas Frescal cheese.
Bruna Leonel Gonçalves; Romulo Dutra Uliana; Sarah Hwa Lee; Carolina Fernanda Coppa; Carlos Augusto Fernandes de Oliveira; Eliana Setsuko Kamimura; Carlos Humberto Corassin. Use of scanning electron microscopy and high-performance liquid chromatography to assess the ability of microorganisms to bind aflatoxin M1 in Minas Frescal cheese. Food Science and Technology 2021, 1 .
AMA StyleBruna Leonel Gonçalves, Romulo Dutra Uliana, Sarah Hwa Lee, Carolina Fernanda Coppa, Carlos Augusto Fernandes de Oliveira, Eliana Setsuko Kamimura, Carlos Humberto Corassin. Use of scanning electron microscopy and high-performance liquid chromatography to assess the ability of microorganisms to bind aflatoxin M1 in Minas Frescal cheese. Food Science and Technology. 2021; ():1.
Chicago/Turabian StyleBruna Leonel Gonçalves; Romulo Dutra Uliana; Sarah Hwa Lee; Carolina Fernanda Coppa; Carlos Augusto Fernandes de Oliveira; Eliana Setsuko Kamimura; Carlos Humberto Corassin. 2021. "Use of scanning electron microscopy and high-performance liquid chromatography to assess the ability of microorganisms to bind aflatoxin M1 in Minas Frescal cheese." Food Science and Technology , no. : 1.
Emerging decontamination technologies have been attracted considerable attention to address the consumers' demand for high quality and safe food products. As one of the important foods in the human diet, cereals are usually stored for long periods, resulting in an increased risk of contamination by different hazards. Mycotoxins comprise one of the significant contaminants of cereals that lead to enormous economic losses to the industry and threats to human health. While prevention is the primary approach towards reducing human exposure to mycotoxins, decontamination methods have also been developed as complementary measures. However, some conventional methods (chemical treatments) do not fulfill industries' expectations due to limitations like safety, efficiency, and the destruction of food quality attributes. In this regard, novel techniques have been proposed to food to comply with the industry's demand and overcome conventional methods' limitations. Novel techniques have different efficiencies for removing or reducing mycotoxins depending on processing conditions, type of mycotoxin, and the food matrix. Therefore, this review provides an overview of novel mycotoxin decontamination technologies such as cold plasma, irradiation, and pulse light, which can be efficient for reducing mycotoxins with minimum adverse effects on the quality and nutritional properties of produce.
Shabir Ahmad Mir; B.N. Dar; Manzoor Ahmad Shah; Sajad Ahmad Sofi; Afshan Mumtaz Hamdani; Carlos A.F. Oliveira; Motahareh Hashemi Moosavi; Amin Mousavi Khaneghah; Anderson S. Sant'Ana. Application of new technologies in decontamination of mycotoxins in cereal grains: Challenges, and perspectives. Food and Chemical Toxicology 2021, 148, 111976 .
AMA StyleShabir Ahmad Mir, B.N. Dar, Manzoor Ahmad Shah, Sajad Ahmad Sofi, Afshan Mumtaz Hamdani, Carlos A.F. Oliveira, Motahareh Hashemi Moosavi, Amin Mousavi Khaneghah, Anderson S. Sant'Ana. Application of new technologies in decontamination of mycotoxins in cereal grains: Challenges, and perspectives. Food and Chemical Toxicology. 2021; 148 ():111976.
Chicago/Turabian StyleShabir Ahmad Mir; B.N. Dar; Manzoor Ahmad Shah; Sajad Ahmad Sofi; Afshan Mumtaz Hamdani; Carlos A.F. Oliveira; Motahareh Hashemi Moosavi; Amin Mousavi Khaneghah; Anderson S. Sant'Ana. 2021. "Application of new technologies in decontamination of mycotoxins in cereal grains: Challenges, and perspectives." Food and Chemical Toxicology 148, no. : 111976.
Consumption of foods containing mycotoxins, as crucial groups of naturally occurring toxic agents, could pose significant health risks. While the extensive scientific literature indicates that prevention of contamination by toxigenic fungi is one of the best ways to reduce mycotoxins, detoxifying strategies are useful for improving the safety of food products. Nowadays, the food and pharmaceutical industries are using the concept of combined technologies to enhance the product yield by implementing emerging techniques, such as ultrasound, ohmic heating, moderate electric field (MEF), pulsed electric field (PEF) and high-pressure processing, during the fermentation process. While the application of emerging technologies in improving the fermentation process is well explained in this literature, there is a lack of scientific texts discussing the possibility of mycotoxin degradation through the interaction effects of emerging technologies and fermentation. Therefore, this study was undertaken to provide deep insight into applying emerging processing technologies in fermentation, mechanisms and the prospects of innovative combinations of physical and biological techniques for mycotoxins' detoxification. Among various emerging technologies, ultrasound, ohmic heating, MEF, PEF, and cold plasma have shown significant positive effects on fermentation and mycotoxins detoxification, highlighting the possibility of interactions from such combinations to degrade mycotoxins in foods.
Mohsen Gavahian; Girish N. Mathad; Carlos A.F. Oliveira; Amin Mousavi Khaneghah. Combinations of emerging technologies with fermentation: Interaction effects for detoxification of mycotoxins? Food Research International 2021, 141, 110104 .
AMA StyleMohsen Gavahian, Girish N. Mathad, Carlos A.F. Oliveira, Amin Mousavi Khaneghah. Combinations of emerging technologies with fermentation: Interaction effects for detoxification of mycotoxins? Food Research International. 2021; 141 ():110104.
Chicago/Turabian StyleMohsen Gavahian; Girish N. Mathad; Carlos A.F. Oliveira; Amin Mousavi Khaneghah. 2021. "Combinations of emerging technologies with fermentation: Interaction effects for detoxification of mycotoxins?" Food Research International 141, no. : 110104.
In this opinion article, the most recent advances in experimental food processing technologies to reduce mycotoxins' levels in food products are presented and discussed regarding their limitations and future challenges for application in food industries. Electron beam irradiation, cold atmospheric plasma, pulsed electric fields, and ozone application have been categorized among the most promising innovative techniques for decontamination of aflatoxins, ochratoxin A, fumonisins, zearalenone, and trichothecenes in food materials. However, these techniques' cost-benefit relationships need to be evaluated for each target food before incorporation by the food industry. Besides, considerable research is necessary to clarify these innovative technologies’ detoxification mechanisms, quantify alterations of the nutritional value of treated foods, and determine potential interactions of compounds originated by mycotoxin degradation with the food matrix under a safety perspective.
Valéria Mr Nunes; Motahareh Moosavi; Amin Mousavi Khaneghah; Carlos Af Oliveira. Innovative modifications in food processing to reduce the levels of mycotoxins. Current Opinion in Food Science 2020, 38, 155 -161.
AMA StyleValéria Mr Nunes, Motahareh Moosavi, Amin Mousavi Khaneghah, Carlos Af Oliveira. Innovative modifications in food processing to reduce the levels of mycotoxins. Current Opinion in Food Science. 2020; 38 ():155-161.
Chicago/Turabian StyleValéria Mr Nunes; Motahareh Moosavi; Amin Mousavi Khaneghah; Carlos Af Oliveira. 2020. "Innovative modifications in food processing to reduce the levels of mycotoxins." Current Opinion in Food Science 38, no. : 155-161.
Mycotoxins are toxic metabolites naturally produced by some fungi species, which cause severe health effects in humans and animals. Although contaminated foods are major routes of human exposure to mycotoxins, occupational exposure may be significant in certain environments contaminated with toxigenic fungi and mycotoxin. This review provides an overview on the occurrence of toxigenic fungi and mycotoxins in workplaces, including recent data on biomarkers used for human biomonitoring of mycotoxins in exposed workers. Feed mill workers had the greatest occupational exposure to mycotoxins, especially aflatoxins, stressing the need for preventive measures to avoid health risks in agricultural-related workplaces.
Larissa Tuanny Franco; Amir Ismail; Adnan Amjad; Carlos Augusto Fernandes De Oliveira. Occurrence of toxigenic fungi and mycotoxins in workplaces and human biomonitoring of mycotoxins in exposed workers: a systematic review. Toxin Reviews 2020, 1 -16.
AMA StyleLarissa Tuanny Franco, Amir Ismail, Adnan Amjad, Carlos Augusto Fernandes De Oliveira. Occurrence of toxigenic fungi and mycotoxins in workplaces and human biomonitoring of mycotoxins in exposed workers: a systematic review. Toxin Reviews. 2020; ():1-16.
Chicago/Turabian StyleLarissa Tuanny Franco; Amir Ismail; Adnan Amjad; Carlos Augusto Fernandes De Oliveira. 2020. "Occurrence of toxigenic fungi and mycotoxins in workplaces and human biomonitoring of mycotoxins in exposed workers: a systematic review." Toxin Reviews , no. : 1-16.
This study aimed to evaluate the effect of Lactobacillus rhamnosus GG on growth of Staphylococcus aureus and Listeria monocytogenes, inoculated alone or in combination on surface of Minas Frescal cheeses, during storage for 21 days at 7 °C. Survival percentages of each individual bacterial species after exposure to in vitro simulated gastrointestinal conditions (SGC) were also determined. The addition of L. rhamnosus did not affect (P > 0.05) pH, moisture, fat, protein and texture profile of Minas Frescal cheeses. L. rhamnosus was able to survive in suitable counts (>6 Log CFU/g) in cheeses from the 7th day of storage, with high survival (>74.6–86.4%) after SGC. An inhibitory effect of L. rhamnosus on L. monocytogenes was observed in cheeses (decrease of 1.1–1.6 Log CFU/g) and after SGC (20% reduction in the survival). No inhibitory effect of L. rhamnosus was observed on S. aureus counts (P > 0.05), and this microorganism did not survive the exposure to SGC. In conclusion, the addition of L. rhamnosus in Minas Frescal cheese has a potential for L. monocytogenes inhibition. Further studies are necessary to elucidate the mechanisms involved in the inhibition process and determine the survival ability of the bacterial species evaluated in in vivo experiments.
Ligia E. Prezzi; Sarah H.I. Lee; Valéria M.R. Nunes; Carlos H. Corassin; Tatiana C. Pimentel; Ramon S. Rocha; Gustavo L.P.A. Ramos; Jonas T. Guimarães; Celso F. Balthazar; Maria Carmela K.H. Duarte; Mônica Q. Freitas; Erick A. Esmerino; Marcia C. Silva; Adriano G. Cruz; Carlos A.F. Oliveira. Effect of Lactobacillus rhamnosus on growth of Listeria monocytogenes and Staphylococcus aureus in a probiotic Minas Frescal cheese. Food Microbiology 2020, 92, 103557 .
AMA StyleLigia E. Prezzi, Sarah H.I. Lee, Valéria M.R. Nunes, Carlos H. Corassin, Tatiana C. Pimentel, Ramon S. Rocha, Gustavo L.P.A. Ramos, Jonas T. Guimarães, Celso F. Balthazar, Maria Carmela K.H. Duarte, Mônica Q. Freitas, Erick A. Esmerino, Marcia C. Silva, Adriano G. Cruz, Carlos A.F. Oliveira. Effect of Lactobacillus rhamnosus on growth of Listeria monocytogenes and Staphylococcus aureus in a probiotic Minas Frescal cheese. Food Microbiology. 2020; 92 ():103557.
Chicago/Turabian StyleLigia E. Prezzi; Sarah H.I. Lee; Valéria M.R. Nunes; Carlos H. Corassin; Tatiana C. Pimentel; Ramon S. Rocha; Gustavo L.P.A. Ramos; Jonas T. Guimarães; Celso F. Balthazar; Maria Carmela K.H. Duarte; Mônica Q. Freitas; Erick A. Esmerino; Marcia C. Silva; Adriano G. Cruz; Carlos A.F. Oliveira. 2020. "Effect of Lactobacillus rhamnosus on growth of Listeria monocytogenes and Staphylococcus aureus in a probiotic Minas Frescal cheese." Food Microbiology 92, no. : 103557.
The exposure and risk characterization of lactating women to aflatoxins (AFs), fumonisins (FBs), ochratoxin A (OTA) and zearalenone (ZEN) due to consumption of different types of food products in Pirassununga, São Paulo, Brazil, was assessed. Lactating women (N = 74) provided samples of foods stored and available at their households between April-August/2018, totaling 184 samples. Mycotoxins were determined in food samples by liquid chromatography-tandem mass spectrometry. According to findings, 20% (n = 36) of all food samples were contaminated with AFs at median concentrations ranging from 9.2 to 18.5 µg/kg, while OTA was detected only in three samples (rice, bread and pasta) at concentrations of 22.3, 23.8 and 48.7 µg/kg, respectively. ZEN was detected in 34 samples (18%) at median levels of 62–195 µg/kg, and FBs at median levels of 58–1546 µg/kg was observed in 22 samples (12%). Moreover, the concentration of AFs, OTA, ZEN and FBs exceeded their respective maximum permitted levels in 11 (6%), 3 (2%), 8 (4%) and 5 (3%) from total samples, respectively. Twenty-eight samples (15%) were contaminated with two or three types of mycotoxins. Corn products contributed for the highest mean probable daily intakes (PDI) of AFs (0.119 ± 0.193 µg/kg body weight (bw)/day), ZEN (0.325 ± 0.097 µg/kg bw/day) and FBs (2.936 ± 1.541 µg/kg bw/day), while wheat-based products contributed for the highest PDI of OTA (0.035 ± 0.028 µg/kg bw/day). The Margin of Exposure (MoE) value for AFs (3.72) demonstrated a high cancer risk (MoE < 10,000), and the Hazard Quotient (HQ) obtained for OTA (24.66), ZEN (4.24) and total FBs (5.01) also resulted in a non-tolerable risk (HQ > 1) via consumption of the investigated food products. Results of this trial indicate high exposure levels of lactating women to dietary mycotoxins in the studied area, which warrant concern about the possible transfer of residual mycotoxins into breast milk.
Carolina Fernanda S.C. Coppa; Amanda C. Cirelli; Bruna L. Gonçalves; Eliana Maria B. Barnabé; Amin Mousavi Khaneghah; Carlos H. Corassin; Carlos A.F. Oliveira. Dietary exposure assessment and risk characterization of mycotoxins in lactating women: Case study of São Paulo state, Brazil. Food Research International 2020, 134, 109272 .
AMA StyleCarolina Fernanda S.C. Coppa, Amanda C. Cirelli, Bruna L. Gonçalves, Eliana Maria B. Barnabé, Amin Mousavi Khaneghah, Carlos H. Corassin, Carlos A.F. Oliveira. Dietary exposure assessment and risk characterization of mycotoxins in lactating women: Case study of São Paulo state, Brazil. Food Research International. 2020; 134 ():109272.
Chicago/Turabian StyleCarolina Fernanda S.C. Coppa; Amanda C. Cirelli; Bruna L. Gonçalves; Eliana Maria B. Barnabé; Amin Mousavi Khaneghah; Carlos H. Corassin; Carlos A.F. Oliveira. 2020. "Dietary exposure assessment and risk characterization of mycotoxins in lactating women: Case study of São Paulo state, Brazil." Food Research International 134, no. : 109272.
Hadi Atabati; Beheshteh Abouhamzeh; Mohammad-Amin Abdollahifar; Sara Sadat Javadinia; Saeed GharibianBajestanie; Ali Atamaleki; Amir Raoofi; Yadolah Fakhri; Carlos Oliveira; Amin Mousavi Khaneghah. The association between high oral intake of acrylamide and risk of breast cancer: An updated systematic review and meta-analysis. Trends in Food Science & Technology 2020, 100, 155 -163.
AMA StyleHadi Atabati, Beheshteh Abouhamzeh, Mohammad-Amin Abdollahifar, Sara Sadat Javadinia, Saeed GharibianBajestanie, Ali Atamaleki, Amir Raoofi, Yadolah Fakhri, Carlos Oliveira, Amin Mousavi Khaneghah. The association between high oral intake of acrylamide and risk of breast cancer: An updated systematic review and meta-analysis. Trends in Food Science & Technology. 2020; 100 ():155-163.
Chicago/Turabian StyleHadi Atabati; Beheshteh Abouhamzeh; Mohammad-Amin Abdollahifar; Sara Sadat Javadinia; Saeed GharibianBajestanie; Ali Atamaleki; Amir Raoofi; Yadolah Fakhri; Carlos Oliveira; Amin Mousavi Khaneghah. 2020. "The association between high oral intake of acrylamide and risk of breast cancer: An updated systematic review and meta-analysis." Trends in Food Science & Technology 100, no. : 155-163.
Silver nanoparticles (AgNPs) are antimicrobial agents that have a wide spectrum of action, including against pathogenic bacteria and spoilage fungi. However, their mechanism of action is not completely clarified. Nowadays, scientific interest on biological synthesis of AgNPs is growing, with emphasis in their extracellular biosynthesis by microbial cells, as it is the most reliable and ecologically correct method for production, yielding no toxic residues. AgNPs may be incorporated to biodegradable and non-biodegradable polymers for the production of food packages with antimicrobial properties, leading to greater safety and longer shelf life. However, it is important to carry out migration tests for new food packages incorporated with AgNPs, based on the effective levels for their inclusion in the packaging materials.
Emelda Simbine; Larissa Da Cunha Rodrigues; Judite Lapa-Guimarães; Eliana Setsuko Kamimura; Carlos Humberto Corassin; Carlos Augusto Fernandes De Oliveira. Application of silver nanoparticles in food packages: a review. Food Science and Technology 2019, 39, 793 -802.
AMA StyleEmelda Simbine, Larissa Da Cunha Rodrigues, Judite Lapa-Guimarães, Eliana Setsuko Kamimura, Carlos Humberto Corassin, Carlos Augusto Fernandes De Oliveira. Application of silver nanoparticles in food packages: a review. Food Science and Technology. 2019; 39 (4):793-802.
Chicago/Turabian StyleEmelda Simbine; Larissa Da Cunha Rodrigues; Judite Lapa-Guimarães; Eliana Setsuko Kamimura; Carlos Humberto Corassin; Carlos Augusto Fernandes De Oliveira. 2019. "Application of silver nanoparticles in food packages: a review." Food Science and Technology 39, no. 4: 793-802.
The composition and occurrence of aflatoxin M1 (AFM1) were investigated in 60 samples of cow's raw milk samples from Razavi Khorasan Province of Iran. Only percentages of milk samples protein, lactose and total solids in samples collected during summer were slightly lower than the Iranian reference values. Compared with summer samples, higher percentages of milk samples components were observed in the winter. The overall mean AFM1 level was 61 ± 8 ng/L, with 24 samples (40%) showing concentrations above the maximum permitted level established in Iran (50 ng/L). Control measures are urgently needed to avoid aflatoxin in milk samples produced in Razavi Khorasan Province.
Marzieh Hajmohammadi; Reza Valizadeh; Abassali Naserian; Mohammad E Nourozi; Ramon S Rocha; Carlos A F Oliveira. Composition and occurrence of aflatoxin M 1 in cow's milk samples from Razavi Khorasan Province, Iran. International Journal of Dairy Technology 2019, 73, 40 -45.
AMA StyleMarzieh Hajmohammadi, Reza Valizadeh, Abassali Naserian, Mohammad E Nourozi, Ramon S Rocha, Carlos A F Oliveira. Composition and occurrence of aflatoxin M 1 in cow's milk samples from Razavi Khorasan Province, Iran. International Journal of Dairy Technology. 2019; 73 (1):40-45.
Chicago/Turabian StyleMarzieh Hajmohammadi; Reza Valizadeh; Abassali Naserian; Mohammad E Nourozi; Ramon S Rocha; Carlos A F Oliveira. 2019. "Composition and occurrence of aflatoxin M 1 in cow's milk samples from Razavi Khorasan Province, Iran." International Journal of Dairy Technology 73, no. 1: 40-45.
Fumonisins (FBs) are mycotoxins produced by Fusarium species that can contaminate human food and animal feed. Due to the harmful effects of FBs on animals, the European Union (EU) defined a recommendation of a maximum of 5 mg FBs (B1 + B2)/kg for complete feed for swine and 1 µg FBs/kg body weight per day as the tolerable daily intake for humans. The aim of this study was to evaluate the toxicity of dietary exposure to low doses of FBs, including a dose below the EU regulatory limits. Four groups of 24 weaned castrated male piglets were exposed to feed containing 0, 3.7, 8.1, and 12.2 mg/kg of FBs for 28 days; the impact was measured by biochemical analysis and histopathological observations. Dietary exposure to FBs at a low dose (3.7 mg/kg of feed) significantly increased the plasma sphinganine-to-sphingosine ratio. FBs-contaminated diets led to histological modifications in the intestine, heart, lung, lymphoid organs, kidney, and liver. The histological alterations in the heart and the intestine appeared at the lowest dose of FBs-contaminated diet (3.7 mg/kg feed) and in the kidney at the intermediate dose (8.1 mg/kg feed). At the highest dose tested (12.2 mg/kg feed), all the organs displayed histological alterations. This dose also induced biochemical modifications indicative of kidney and liver alterations. In conclusion, our data indicate that FBs-contaminated diets at doses below the EU regulatory limit cause histological lesions in several organs. This study suggests that EU recommendations for the concentration of FBs in animal feed, especially for swine, are not sufficiently protective and that regulatory doses should be modified for better protection of animal health.
Chloé Terciolo; Ana Paula Bracarense; Pollyana C.M.C. Souto; Anne-Marie Cossalter; Léonie Dopavogui; Nicolas Loiseau; Carlos A. F. Oliveira; Philippe Pinton; Isabelle P. Oswald. Fumonisins at Doses below EU Regulatory Limits Induce Histological Alterations in Piglets. Toxins 2019, 11, 548 .
AMA StyleChloé Terciolo, Ana Paula Bracarense, Pollyana C.M.C. Souto, Anne-Marie Cossalter, Léonie Dopavogui, Nicolas Loiseau, Carlos A. F. Oliveira, Philippe Pinton, Isabelle P. Oswald. Fumonisins at Doses below EU Regulatory Limits Induce Histological Alterations in Piglets. Toxins. 2019; 11 (9):548.
Chicago/Turabian StyleChloé Terciolo; Ana Paula Bracarense; Pollyana C.M.C. Souto; Anne-Marie Cossalter; Léonie Dopavogui; Nicolas Loiseau; Carlos A. F. Oliveira; Philippe Pinton; Isabelle P. Oswald. 2019. "Fumonisins at Doses below EU Regulatory Limits Induce Histological Alterations in Piglets." Toxins 11, no. 9: 548.