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Dr. Solomiia Kozachok
Department of Biochemistry and Crop Quality Institute of Soil Science and Plant Cultivation, State Research Instituteul. Czartoryskich, Pulawy, Poland

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0 LC-MS
0 Mycotoxins
0 Phytochemistry
0 phenolic compounds

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Journal article
Published: 27 July 2021 in Phytochemistry
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The study is based on phytochemical profiling and in vitro evaluation of biological effects of phenolic acid derivatives-rich Herniaria fractions, isolated from two rupturewort (Herniaria L.) species, i.e. Herniaria incana Lam. (syn. H. besseri Fisch. ex Hornem) and H. polygama J. Gay (syn. H. odorata). For the first time, the composition of phenolic compounds of these species was extensively evaluated by both LC-HR-QTOF-ESI-MS and Nuclear Magnetic Resonance spectroscopy (NMR). LC-MS analyses of H. polygama revealed 72 tentatively identified compounds, while H. incana – 63. Only 8% of the metabolites reported in this work have been previously described for Herniaria spp. Most of the identified specialized metabolites were cinnamic and benzoic acid derivatives. Phenolic fraction of H. incana herb contained flavonoids as well. A multi-step chromatographic separation of phenolic fractions from H. polygama yielded three known cinnamic and one benzoic acid derivates, and from H. incana – 4 known flavonoids and one previously undescribed, i.e. rhamnocitrin-3-O-[3-hydroxy-3-methylglutaryl-(1 → 6′′)]-[α-rhamnopyranosyl-(1 → 2′′)]-β-glucopyranoside. Antioxidant properties of the examined fractions (1–50 μg/ml) were assessed in human blood plasma under the conditions of peroxynitrite-induced oxidative stress. Measurements of well-known biomarkers such as 3-nitrotyrosine, protein thiol groups, thiobarbituric acid-reactive substances and the ferric reducing ability of blood plasma revealed the protective effect of Herniaria fractions against oxidative damage to blood plasma components. Furthermore, the examined fractions effectively ameliorated the inflammatory response of the concanavalin A-stimulated human peripheral blood mononuclear cells (PBMCs). Additionally, cellular safety of the fractions was confirmed in PBMCs.

ACS Style

Joanna Kolodziejczyk-Czepas; Solomiia Kozachok; Łukasz Pecio; Svitlana Marchyshyn; Wiesław Oleszek. Determination of phenolic profiles of Herniaria polygama and Herniaria incana fractions and their in vitro antioxidant and anti-inflammatory effects. Phytochemistry 2021, 190, 112861 .

AMA Style

Joanna Kolodziejczyk-Czepas, Solomiia Kozachok, Łukasz Pecio, Svitlana Marchyshyn, Wiesław Oleszek. Determination of phenolic profiles of Herniaria polygama and Herniaria incana fractions and their in vitro antioxidant and anti-inflammatory effects. Phytochemistry. 2021; 190 ():112861.

Chicago/Turabian Style

Joanna Kolodziejczyk-Czepas; Solomiia Kozachok; Łukasz Pecio; Svitlana Marchyshyn; Wiesław Oleszek. 2021. "Determination of phenolic profiles of Herniaria polygama and Herniaria incana fractions and their in vitro antioxidant and anti-inflammatory effects." Phytochemistry 190, no. : 112861.

Journal article
Published: 24 February 2021 in Molecules
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Tagetes erecta L. is a popular ornamental plant of the Asteraceae family, which is widely cultivated not only for its decorative use, but also for the extraction of lutein. Besides carotenoid representatives, which have been extensively studied, other important classes of secondary metabolites present in the plant, such as polyphenols, could exhibit important biological activities. The phytochemical analysis of a methanolic extract obtained from T. erecta inflorescences was achieved using liquid chromatography–mass spectrometry (LC-MS) techniques. The extract was further subjected to a multistep purification process, which allowed the separation of different fractions. The total extract and its fractions contain several polyphenolic compounds, such as hydroxybenzoic and hydroxycinnamic acid derivatives, flavonols (especially quercetagetin glycosides), and several aglycons (e.g., quercetin, patuletin). One of the fractions, containing mostly quercetagitrin, was subjected to two different antioxidant assays (metal chelating activity and lipoxygenase inhibition) and to in vitro cytotoxicity assessment. Generally, the biological assays showed promising results for the investigated fraction compared to the initial extract. Given the encouraging outcome of the in vitro assays, further purification and structural analysis of compounds from T. erecta extracts, as well as further in vivo investigations are justified.

ACS Style

Ana Burlec; Łukasz Pecio; Solomiia Kozachok; Cornelia Mircea; Andreia Corciovă; Liliana Vereștiuc; Oana Cioancă; Wiesław Oleszek; Monica Hăncianu. Phytochemical Profile, Antioxidant Activity, and Cytotoxicity Assessment of Tagetes erecta L. Flowers. Molecules 2021, 26, 1201 .

AMA Style

Ana Burlec, Łukasz Pecio, Solomiia Kozachok, Cornelia Mircea, Andreia Corciovă, Liliana Vereștiuc, Oana Cioancă, Wiesław Oleszek, Monica Hăncianu. Phytochemical Profile, Antioxidant Activity, and Cytotoxicity Assessment of Tagetes erecta L. Flowers. Molecules. 2021; 26 (5):1201.

Chicago/Turabian Style

Ana Burlec; Łukasz Pecio; Solomiia Kozachok; Cornelia Mircea; Andreia Corciovă; Liliana Vereștiuc; Oana Cioancă; Wiesław Oleszek; Monica Hăncianu. 2021. "Phytochemical Profile, Antioxidant Activity, and Cytotoxicity Assessment of Tagetes erecta L. Flowers." Molecules 26, no. 5: 1201.

Journal article
Published: 15 October 2019 in Phytochemistry
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Twelve undescribed triterpenoid pentacyclic glycosides, medicagenic acid (3-O-β-D-glucuronopyranosyl-28-O-{[β-D-glucopyranosyl-(1 → 3)-α-L-rhamnopyranosyl-(1 → 2)]-[α-L-rhamnopyranosyl-(1 → 3)]-4-O-acetyl-β-D-fucopyranosyl-(1→)}-2β,3β-dihydroxyolean-12-ene-23,28-dioic acid, 3-O-β-D-glucuronopyranosyl-28-O-{[α-L-rhamnopyranosyl-(1 → 2)]-[β-D-apiofuranosyl-(1 → 3)]-4-O-acetyl-β-D-fucopyranosyl-(1→)}-2β,3β-dihydroxyolean-12-ene-23,28-dioic acid, 3-O-β-D-glucuronopyranosyl-28-O-{[α-L-rhamnopyranosyl-(1 → 2)]-3,4-O-diacetyl-β-D-fucopyranosyl-(1→)}-2β,3β-dihydroxyolean-12-ene-23,28-dioic acid, 28-O-{[6-O-acetyl-β-D-glucopyranosyl-(1 → 2)]-[2-O-acetyl-α-L-rhamnopyranosyl-(1 → 4)-β-D-glucopyranosyl-(1 → 6)]-β-D-glucopyranosyl-(1→)}-2β,3β-dihydroxyolean-12-ene-23,28-dioic acid, 28-O-{[6-O-acetyl-β-D-glucopyranosyl-(1 → 2)]-[3-O-acetyl-α-L-rhamnopyranosyl-(1 → 4)-β-D-glucopyranosyl-(1 → 6)]-β-D-glucopyranosyl-(1→)}-2β,3β-dihydroxyolean-12-ene-23,28-dioic acid, 28-O-{[6-O-acetyl-β-D-glucopyranosyl-(1 → 2)]-[4-O-acetyl-α-L-rhamnopyranosyl-(1 → 4)-β-D-glucopyranosyl-(1 → 6)]-β-D-glucopyranosyl-(1→)}-2β,3β-dihydroxyolean-12-ene-23,28-dioic acid, 28-O-{[6-O-acetyl-β-D-glucopyranosyl-(1 → 2)]-[β-D-glucopyranosyl-(1 → 6)]-β-D-glucopyranosyl-(1→)}-2β,3β-dihydroxyolean-12-ene-23,28-dioic acid, 28-O-{[β-D-glucopyranosyl-(1 → 2)]-[β-D-glucopyranosyl-(1 → 6)]-β-D-glucopyranosyl-(1→)}-2β,3β-dihydroxyolean-12-ene-23,28-dioic acid), zanhic acid (3-O-β-D-glucuronopyranosyl-28-O-{[β-D-glucopyranosyl-(1 → 3)-α-L-rhamnopyranosyl-(1 → 2)]-[α-L-rhamnopyranosyl-(1 → 3)]-4-O-acetyl-β-D-fucopyranosyl-(1→)}2β,3β,16α-trihydroxyolean-12-ene-23,28-dioic acid, 3-O-β-D-glucuronopyranosyl-28-O-{[β-D-glucopyranosyl-(1 → 3)-α-L-rhamnopyranosyl-(1 → 2)]-β-D-fucopyranosyl-(1→)}-2β,3β,16α-trihydroxyolean-12-ene-23,28-dioic acid), 29-hydroxy-medicagenic acid (3-O-β-D-glucuronopyranosyl-28-O-{[β-D-glucopyranosyl-(1 → 3)-α-L-rhamnopyranosyl-(1 → 2)]-[α-L-rhamnopyranosyl-(1 → 3)]-4-O-acetyl-β-D-fucopyranosyl-(1→)}-2β,3β,29β-trihydroxyolean-12-ene-23,28-dioic acid) and herniaric acid (28-O-{[6-O-acetyl-β-D-glucopyranosyl-(1 → 2)]-[α-L-rhamnopyranosyl-(1 → 4)-β-D-glucopyranosyl-(1 → 6)]-β-D-glucopyranosyl-(1→)}-2β,3β-dihydroxyolean-18-ene-23,28-dioic acid) were isolated from the whole plant extract of Herniaria glabra L. (Caryophyllaceae), wild growing in the Ukraine. In addition, five known triterpenoid saponins; i.e. herniariasaponins 1, 4, 5, 6, and 7 were also isolated. Their structures were elucidated by HRESIMS, 1D and 2D NMR spectroscopy, as well as by comparison with the literature data. Twelve herniariasaponins, the purified crude extract, and the saponin fraction were evaluated in vitro for their xanthine oxidase, collagenase, elastase, and tyrosinase inhibitory activity. Moreover, herniariasaponins 4, 5, and 7 were screened for their cholinesterase inhibitory potential. As a result, no or low inhibition towards the mentioned enzymes was observed.

ACS Style

Solomiia Kozachok; Łukasz Pecio; Ilkay Erdogan Orhan; Fatma Sezer Senol Deniz; Svitlana Marchyshyn; Wiesław Oleszek. Reinvestigation of Herniaria glabra L. saponins and their biological activity. Phytochemistry 2019, 169, 112162 .

AMA Style

Solomiia Kozachok, Łukasz Pecio, Ilkay Erdogan Orhan, Fatma Sezer Senol Deniz, Svitlana Marchyshyn, Wiesław Oleszek. Reinvestigation of Herniaria glabra L. saponins and their biological activity. Phytochemistry. 2019; 169 ():112162.

Chicago/Turabian Style

Solomiia Kozachok; Łukasz Pecio; Ilkay Erdogan Orhan; Fatma Sezer Senol Deniz; Svitlana Marchyshyn; Wiesław Oleszek. 2019. "Reinvestigation of Herniaria glabra L. saponins and their biological activity." Phytochemistry 169, no. : 112162.

Journal article
Published: 20 June 2019 in Toxins
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The phytochemical constituents of apple waste were established as potential antifungal agents against four crops pathogens, specifically, Botrytis sp., Fusarium oxysporum, Petriella setifera, and Neosartorya fischeri. Crude, purified extracts and fractions of apple pomace were tested in vitro to evaluate their antifungal and antioxidant properties. The phytochemical constituents of the tested materials were mainly represented by phloridzin and quercetin derivatives, as well as previously undescribed in apples, monoterpene–pinnatifidanoside D. Its structure was confirmed by 1D- and 2D-nuclear magnetic resonance (NMR) spectroscopic analyses. The fraction containing quercetin pentosides possessed the highest antioxidant activity, while the strongest antifungal activity was exerted by a fraction containing phloridzin. Sugar moieties differentiated the antifungal activity of quercetin glycosides. Quercetin hexosides possessed stronger antifungal activity than quercetin pentosides.

ACS Style

Marta Oleszek; Łukasz Pecio; Solomiia Kozachok; Żaneta Lachowska-Filipiuk; Karolina Oszust; Magdalena Frąc. Phytochemicals of Apple Pomace as Prospect Bio-Fungicide Agents against Mycotoxigenic Fungal Species—In Vitro Experiments. Toxins 2019, 11, 361 .

AMA Style

Marta Oleszek, Łukasz Pecio, Solomiia Kozachok, Żaneta Lachowska-Filipiuk, Karolina Oszust, Magdalena Frąc. Phytochemicals of Apple Pomace as Prospect Bio-Fungicide Agents against Mycotoxigenic Fungal Species—In Vitro Experiments. Toxins. 2019; 11 (6):361.

Chicago/Turabian Style

Marta Oleszek; Łukasz Pecio; Solomiia Kozachok; Żaneta Lachowska-Filipiuk; Karolina Oszust; Magdalena Frąc. 2019. "Phytochemicals of Apple Pomace as Prospect Bio-Fungicide Agents against Mycotoxigenic Fungal Species—In Vitro Experiments." Toxins 11, no. 6: 361.

Journal article
Published: 11 April 2019 in Molecules
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The effect of elicitation with jasmonic acids (JA) and yeast extract (YE) on the production of phenolic compounds as well as the antioxidant and anti-inflammatory properties of phenolic extracts of lovage was evaluated. The analysis of phenolic compounds carried out with the UPLC-MS technique indicated that rutin was the dominant flavonoid, while 5-caffeoylquinic acid was the main component in the phenolic acid fraction in the lovage leaves. The application of 10 µM JA increased the content of most of the identified phenolic compounds. The highest antioxidant activities estimated as free radical scavenging activity against ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) and reducing power were determined for the sample elicited with 10 µM JA, while this value determined as iron chelating ability was the highest for the 0.1% YE-elicited lovage. The 0.1% and 1% YE elicitation also caused significant elevation of the lipoxygenase (LOX) inhibition ability, while all the concentrations of the tested elicitors significantly improved the ability to inhibit cyclooxygenase 2 (COX2) (best results were detected for the 10 µM JA and 0.1% YE2 sample). Thus, 0.1% yeast extract and 10 µM jasmonic acid proved to be most effective in elevation of the biological activity of lovage.

ACS Style

Urszula Złotek; Urszula Szymanowska; Łukasz Pecio; Solomiia Kozachok; Anna Jakubczyk. Antioxidative and Potentially Anti-inflammatory Activity of Phenolics from Lovage Leaves Levisticum officinale Koch Elicited with Jasmonic Acid and Yeast Extract. Molecules 2019, 24, 1441 .

AMA Style

Urszula Złotek, Urszula Szymanowska, Łukasz Pecio, Solomiia Kozachok, Anna Jakubczyk. Antioxidative and Potentially Anti-inflammatory Activity of Phenolics from Lovage Leaves Levisticum officinale Koch Elicited with Jasmonic Acid and Yeast Extract. Molecules. 2019; 24 (7):1441.

Chicago/Turabian Style

Urszula Złotek; Urszula Szymanowska; Łukasz Pecio; Solomiia Kozachok; Anna Jakubczyk. 2019. "Antioxidative and Potentially Anti-inflammatory Activity of Phenolics from Lovage Leaves Levisticum officinale Koch Elicited with Jasmonic Acid and Yeast Extract." Molecules 24, no. 7: 1441.

Journal article
Published: 01 August 2018 in Phytochemistry
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The phytochemical investigation of the whole plant extracts of Herniaria glabra L. (Caryophyllaceae) led to the identification and isolation of four known flavonoids, one known and three undescribed maltol derivatives, and benzyl β-gentiobioside. The structures were established by extensive 1D and 2D NMR spectroscopic analyses, as well as HRESIMS data. For the first time in Herniaria genus, as well as in Caryophylaceae family the presence of apiorutin {quercetin 3-O-[(D-apio-β-d-furanosyl-(1 → 2)-O-[-α-l-rhamnopyranosyl-(1 → 6)]-β-d-glucopyranoside]} and licoagroside B {maltol 3-O-[6-O-(3-hydroxy-3-methylglutaroyl)]-β-d-glucopyranoside} were revealed. Additionally, antioxidant actions of apiorutin, rutin, narcissin (isorhamentin 3-O-β-d-rutinoside) and licoagroside B were assessed in human blood plasma, exposed to the peroxynitrite-induced oxidative stress in vitro. The isolates partly reduced oxidative (oxidation of thiol groups) and nitrative (tyrosine nitration) damage to blood plasma proteins, decreased plasma lipid peroxidation as well as enhanced the non-enzymatic antioxidant capacity of blood plasma. No cytotoxicity of the examined substances towards peripheral blood mononuclear cells was found.

ACS Style

Solomiia Kozachok; Łukasz Pecio; Joanna Kolodziejczyk-Czepas; Svitlana Marchyshyn; Pawel Nowak; Jaroslaw Moldoch; Wieslaw Oleszek. γ-Pyrone compounds: flavonoids and maltol glucoside derivatives from Herniaria glabra L. collected in the Ternopil region of the Ukraine. Phytochemistry 2018, 152, 213 -222.

AMA Style

Solomiia Kozachok, Łukasz Pecio, Joanna Kolodziejczyk-Czepas, Svitlana Marchyshyn, Pawel Nowak, Jaroslaw Moldoch, Wieslaw Oleszek. γ-Pyrone compounds: flavonoids and maltol glucoside derivatives from Herniaria glabra L. collected in the Ternopil region of the Ukraine. Phytochemistry. 2018; 152 ():213-222.

Chicago/Turabian Style

Solomiia Kozachok; Łukasz Pecio; Joanna Kolodziejczyk-Czepas; Svitlana Marchyshyn; Pawel Nowak; Jaroslaw Moldoch; Wieslaw Oleszek. 2018. "γ-Pyrone compounds: flavonoids and maltol glucoside derivatives from Herniaria glabra L. collected in the Ternopil region of the Ukraine." Phytochemistry 152, no. : 213-222.

Article
Published: 01 July 2018 in International Agrophysics
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Extracts of Solidago virgaurea L. (European goldenrod), Lavandula angustifolia Mill. (lavender) and Arnica chamissonis Less. (arnica) were tested as potential additives for methane fermentation in bioreactors, as it was hypothesized that their antioxidant properties may improve biogas production efficiency. Hence, methane fermentation of maize silage with the addition of tested extracts was performed in eudiometers, and both the biogas volume and methane content in biogas were measured. In addition, antioxidant properties, such as reducing power and 1,1-diphenyl-2-picrylhydrazyl free radical-scavenging activity, were determined spectrophotometrically. The results showed that only the addition of a goldenrod crude extract caused a significant increase in biogas yield, despite the fact that it presented the lowest antioxidant activity of the three tested species. No significant differences in biogas yield were found between the other two additives and the control. Biogas production efficiency was not correlated with the antioxidant activity of the crude extracts. Hence, the increase in methane fermentation efficiency in the case of goldenrod supplementation might have resulted from some other non-antioxidant compounds occurring in this species.

ACS Style

Marta Oleszek; Solomiia Kozachok. Antioxidant activity of plant extracts and their effect on methane fermentation in bioreactors. International Agrophysics 2018, 32, 395 -401.

AMA Style

Marta Oleszek, Solomiia Kozachok. Antioxidant activity of plant extracts and their effect on methane fermentation in bioreactors. International Agrophysics. 2018; 32 (3):395-401.

Chicago/Turabian Style

Marta Oleszek; Solomiia Kozachok. 2018. "Antioxidant activity of plant extracts and their effect on methane fermentation in bioreactors." International Agrophysics 32, no. 3: 395-401.

Journal article
Published: 01 September 2016 in Current Issues in Pharmacy and Medical Sciences
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The plants from Herniaria genus (Caryophyllaceae family) are mainly applied as diuretic agents for the treatment of kidney and gall-stones, gouts, urinary tract infections, hypertension and diabetes. The most widely spread species in Europe is Herniaria glabra L. (HG). Herniaria polygama J.Gay (HP) is found growing from Eastern Europe to Asia. Surprisingly, no work has been reported on the analysis of primary metabolites of HP yet and there is only a limited data on HG. The aim of our study was to establish the monosaccharide composition after a complete hydrolysis and in a free state in the entire herbs of HG and HP harvested in the western and central parts of Ukraine. The carbohydrates were separated by gas chromatography-mass spectrometry after conversion into volatile derivatives as aldononitrile acetate. As a result, the monosaccharide composition after a total hydrolysis was established with the contribution of the following components: D-rhamnose, D-arabinose, D-fucose, D-xylose, D-manose, D-glucose, D-galactose, D-pinitol, myo-inisitol, D-mannitol, D-dulcitol. In a free state it was detected: D-fructose, D-glucose, D-galactose, D-pinitol, myo-inisitol, D-mannitol and D-saccharose. The following monosaccharides were found in the most abundant quantities in HG and HP respectively: glucose was determined as the major component – 33.40 and 22.80 mg/g, the second dominant sugar was pinitol – 16.80 and 18.07 mg/g, followed by galactose – 13.88 mg/g in HG and arabinose – 8.13 mg/g in HP. Sugars were determined in these plant species for the first time and this finding shed new light on their pharmacological application.

ACS Style

Solomiia Kozachok; Svitlana Marchyshyn; Andriy Ostapchuk; Liudmyla Zavyalova. Monosaccharide composition of Herniaria glabra L. and Herniaria polygama J.Gay. Current Issues in Pharmacy and Medical Sciences 2016, 29, 142 -144.

AMA Style

Solomiia Kozachok, Svitlana Marchyshyn, Andriy Ostapchuk, Liudmyla Zavyalova. Monosaccharide composition of Herniaria glabra L. and Herniaria polygama J.Gay. Current Issues in Pharmacy and Medical Sciences. 2016; 29 (3):142-144.

Chicago/Turabian Style

Solomiia Kozachok; Svitlana Marchyshyn; Andriy Ostapchuk; Liudmyla Zavyalova. 2016. "Monosaccharide composition of Herniaria glabra L. and Herniaria polygama J.Gay." Current Issues in Pharmacy and Medical Sciences 29, no. 3: 142-144.