Techniques based on plasmonic resonance can provide label-free, signal enhanced, and real-time sensing means for bioparticles and bioprocesses at the molecular level. With the development in nanofabrication and material science, plasmonics based on synthesized nanoparticles and manufactured nano-patterns in thin films have been prosperously explored. In this short review, resonance modes, materials, and hybrid functions by simultaneously using electrical conductivity for plasmonic biosensing techniques are exclusively reviewed for designs containing nanovoids in thin films. This type of plasmonic biosensors provide prominent potential to achieve integrated lab-on-a-chip which is capable of transporting and detecting minute of multiple bio-analytes with extremely high sensitivity, selectivity, multi-channel and dynamic monitoring for the next generation of point-of-care devices.

We have used a gold nanohole array to trap single polystyrene nanoparticles, with a mean diameter of 30 nm, into separated hot spots located at connecting nanoslot regions. A high trap stiffness of approximately $0.85\mathrm{fN}/(\mathrm{nm}·\mathrm{mW})$ at a low-incident laser intensity of $\sim 0.51\mathrm{mW}/{\mathrm{\mu m}}^2$ at 980 nm was obtained. The experimental results were compared to the simulated trapping force, and a reasonable match was achieved. This plasmonic array is useful for lab-on-a-chip applications and has particular appeal for trapping multiple nanoparticles with predefined separations or arranged in patterns in order to study interactions between them.

The manipulation of microparticles using optical forces has led to many applications in the life and physical sciences. To extend optical trapping towards the nano-regime, in this work we demonstrate trapping of single nanoparticles in arrays of plasmonic coaxial nano-apertures with various inner disk sizes and theoretically estimate the associated forces. A high normalized experimental trap stiffness of 3.50 fN nm⁻¹ mW⁻¹ μm⁻² for 20 nm polystyrene particles is observed for an optimum design of 149 nm for the nanodisk diameter at a trapping wavelength of 980 nm. Theoretical simulations are used to interpret the enhancement of the observed trap stiffness. A quick particle trapping time of less than 8 s is obtained at a concentration of 14 × 10¹¹ particles ml⁻¹ with low incident laser intensity of 0.59 mW μm⁻². This good trapping performance with fast delivery of nanoparticles to multiple trapping sites emerges from a combination of the enhanced electromagnetic near-field and spatial temperature increase. This work has applications in nanoparticle delivery and trapping with high accuracy, and bridges the gap between optical manipulation and nanofluidics.

In this work, we demonstrate trapping of microparticles using a plasmonic tweezers based on arrays of annular apertures. The transmission spectra and the E- field distribution are simulated to calibrate the arrays. Theoretically, we observe sharp peaks in the transmission spectra for dipole resonance modes and these are redshifted as the size of the annular aperture is reduced. We also expect an absorption peak at approximately 1,115 µm for the localised plasmon resonance. Using a laser frequency between the two resonances, multiple plasmonic hotspots are created and used to trap and transport micron and submicron particles. Experimentally, we demonstrate trapping of individual 0.5 µm and 1 µm polystyrene particles and particle transportation over the surface of the annular apertures using less than 1.5 mW/µm2 incident laser intensity at 980 nm.

An optical impedance spectroscopy (OIS) technique based on a single-mode electro-active-integrated optical waveguide (EA-IOW) was developed to investigate electron-transfer processes of redox adsorbates. A highly sensitive single-mode EA-IOW device was used to optically follow the time-dependent faradaic current originated from a submonolayer of cytochrome c undergoing redox exchanges driven by a harmonic modulation of the electric potential at several dc bias potentials and at several frequencies. To properly retrieve the faradaic current density from the ac-modulated optical signal, we introduce here a mathematical formalism that (i) accounts for intrinsic changes that invariably occur in the optical baseline of the EA-IOW device during potential modulation and (ii) provides accurate results for the electro-chemical parameters. We are able to optically reconstruct the faradaic current density profile against the dc bias potential in the working electrode, identify the formal potential, and determine the energy-width of the electron-transfer process. In addition, by combining the optically reconstructed faradaic signal with simple electrical measurements of impedance across the whole electrochemical cell and the capacitance of the electric double-layer, we are able to determine the time-constant connected to the redox reaction of the adsorbed protein assembly. For cytochrome c directly immobilized onto the indium tin oxide (ITO) surface, we measured a reaction rate constant of 26.5 s^–1. Finally, we calculate the charge-transfer resistance and pseudocapacitance associated with the electron-transfer process and show that the frequency dependence of the redox reaction of the protein submonolayer follows as expected the electrical equivalent of an RC-series admittance diagram. Above all, we show here that OIS with single-mode EA-IOW’s provide strong analytical signals that can be readily monitored even for small surface-densities of species involved in the redox process (e.g., fmol/cm², 0.1% of a full protein monolayer). This experimental approach, when combined with the analytical formalism described here, brings additional sensitivity, accuracy, and simplicity to electro-chemical analysis and is expected to become a useful tool in investigations of redox processes.