To monitor multiple environmental factors of henhouses in modern chicken farms, a henhouse online monitoring system based on wireless sensor network was developed using wireless sensor technology and computer network technology. Sensor data compensation and correction were designed to be achieved using software and data fitting methods, data reliable transmission achieved using a data loss recovery strategy, and data missing during monitoring addressed using a self-decision and online filling method. Operation test of the system showed that: The system was economic and reliable; it enabled wireless monitoring and Web display of the environmental factors of a henhouse; and the root mean square errors (RMSEs) between the estimated values from the self-decision and on-line filling method and experimental values of the four environmental factors were 0.1698, 3.0859, 77 and 0.094, respectively, indicative of high estimation accuracy. The system can provide support for modern management of henhouses and can be transplanted to related monitoring scenarios in the agricultural field.

Dendrobium officinale is a valuable traditional Chinese herbal plant that is both medicinal and edible. However, the yield of wild Dendrobium officinale is limited. Adverse stress affects the growth, development, and yield of plants, among which low temperature is the primary limiting factor for introducing Dendrobium officinale to high-latitude areas and expanding the planting area. Therefore, this study aims to explore the variations in growth ability, cold resistance, and contents of bioactive compounds among different Dendrobium officinale strains. Four strains of Dendrobium officinale were selected as experimental materials and were subjected to low-temperature stress (4 °C). The agronomic traits, physiological indices, as well as the expressions of cold resistance-related genes (HSP70, DcPP2C5, DoCDPK1, and DoCDPK6) in the roots and leaves of Dendrobium officinale, were determined. The contents of bioactive compounds, including polysaccharides, flavonoids, and phenols were also measured. Compared with the other strains, Xianju had the highest seed germination and transplantation-related survival rates. Under low-temperature stress, Xianju exhibited the strongest cold resistance ability, as revealed by the changes in water contents, chlorophyll levels, electrical conductivities, enzyme activities, and expressions of the cold resistance-related genes. Additionally, the polysaccharide content of Xianju increased the most, while the stem flavonoid and leaf phenol contents were elevated in all four strains under cold treatment. Therefore, selecting excellent performing strains is expected to expand the planting area, improve the yield, and increase the economic benefits of Dendrobium officinale in high latitude areas with lower temperatures.

Straw cellulose is an abundant renewable resource in nature. In recent years, the conversion of cellulose from waste straw into biofuel by specific microorganisms’ fragmentation has attracted extensive attention. Although many bacteria with the ability to degrade cellulose have been identified, comprehensive bioinformatics analyses of these bacteria remain limited, and research exploring optimal fragmentation conditions is scarce. Our study involved the isolation and screening of bacteria from various locations in Yangzhou using carboxymethyl cellulose (CMC) media. Then, the cellulose-degrading bacteria were identified using 16S rRNA and seven candidate bacterial strains with cellulose degrading ability were identified in Yangzhou city for the first time. The cellulase activity was determined by the 3,5-dinitrosalicylic acid (DNS) method in different fragmentation conditions, and finally two bacteria strains with the strongest cellulose degradation ability were selected for whole genome sequencing analysis. Sequencing results revealed that the genome sizes of Rhodococcus wratislaviensis YZ02 and Pseudomonas Xanthosomatis YZ03 were 8.51 Mb and 6.66 Mb, containing 8,466 and 5,745 genes, respectively. A large number of cellulose degradation-related genes were identified and annotated using KEGG, GO and COG analyses. In addition, genomic CAZyme analysis indicated that both R. wratislaviensis YZ02 and P. Xanthosomatis YZ03 harbor a series of glycoside hydrolase family (GH) genes and other genes related to cellulose degradation. Our finding provides new options for the development of cellulose-degrading bacteria and a theoretical basis for improving the cellulose utilization of straw.

Dendrobium officinale Kimura & Migo (D. officinale) is a well-recognized traditional Chinese medicinal herb that is both medicinal and edible. Contemporary pharmacological studies have revealed that D. officinale contains abundant bioactive compounds, including polysaccharides, flavonoids, alkaloids, and dendrobine, exhibiting diverse pharmacological properties such as antioxidant, anti-inflammatory, and immunomodulatory effects. However, the industrial application of D. officinale faces many problems, such as the scarcity of wild resources, low natural reproduction rate, and slow growth rate as well as the lack of relevant industrial standards. Nevertheless, substantial advancements, including the exploitation of artificial propagation techniques and breeding of new varieties, have been achieved in recent years. These developments have effectively addressed the challenges associated with its low natural reproduction rate and the scarcity of wild resources. This review summarizes the progress in the industrial development, seedling cultivation, and pharmacological exploration of D. officinale in recent years. Furthermore, it analyzes current research inadequacies and offers strategic solutions to enhance its application in healthcare and medicine.

To improve the problem of low seeding efficiency facing the single-row planting mode traditionally used in China, this study aimed to design a novel three-row pneumatic precision metering device. The working principle and main structure were introduced in the paper. The three main factors affecting the seeding performance comprised of vacuum pressure, angular velocity of the metering tray, and taper angle of the sucking hole. A Box-Behnken experiment design having the qualified index and missed seeding index as the experimental index was used, and the results indicated that optimal performance of the metering device was achieved when the vacuum pressure was 2.16 kPa, the angular velocity of the metering tray was 29.43 rpm, and the taper angle of the sucking hole was 61.51°. The qualified index of the inner, middle, and outer ring was 95.12%, 94.68% and 94.24% respectively, while the missed seeding index of the inner, middle, and outer ring was 3.67%, 3.12% and 2.58% respectively under the optimal combination of parameters. The bench test results were basically consistent with the optimized results. This paper can provide support for the design of a three-row pneumatic precision metering device for Brassica chinensis.

In present-day mechanized garlic seeding, high missing rates and low qualified percentages of single seeds are common problems; thus, a finger clip plate garlic seed-metering device was designed in this study. First, the structure and working principle of the seed-metering device were studied. Subsequently, the critical component parameters of the seed-metering device were determined using theoretical calculations; then, EDEM software was used in single-factor simulation experiments to analyze the effects of opening the diameter of the seed-collecting spoon, the operating speed of the seeding tray, and the population number on the seed-filling performance. Finally, a Box–Behnken center combination experiment was conducted with the population, opening the diameter of the seed-collecting spoon, and rotating the speed of the seeding tray as experiment factors, with the single-seed filling rate, qualified percent, and missing rate as evaluation indicators. A three-factor and three-level orthogonal test was conducted to establish the mathematic regression model of the experiment factors and evaluation indicators, as well as to realize the parameter optimization. After rounding, the laboratory validation test was carried out with 240~280 seeds, a 26 mm seed scoop-opening diameter, and a 28 r/min operating speed. The average qualified rate, missing rate, and reply rate of single seeds were 91.86%, 2.71%, and 5.43%, respectively, which is basically consistent with the forecast results of regression model.