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Propolis is a resinous substance produced by bees that exhibits antimicrobial, immunostimulatory and antioxidant activity. Its use is common in functional foods, cosmetics and traditional medicine despite the fact that it demonstrates low extraction yields and inconsistency in non-toxic solvents. In this work, a new encapsulation and delivery system consisting of liposomes and cyclodextrins incorporating propolis polyphenols has been developed and characterized. The antioxidant, antimutagenic and antiaging properties of the system under normal and UVB-induced oxidative stress conditions were investigated in cultured skin cells and/or reconstituted skin model. Furthermore, the transcript accumulation for an array of genes involved in many skin-related processes was studied. The system exhibits significant polyphenol encapsulation efficiency, physicochemical stability as well as controlled release rate in appropriate conditions. The delivery system can retain the anti-mutagenic, anti-oxidative and anti-ageing effects of propolis polyphenols to levels similar and comparable to those of propolis methanolic extracts, making the system ideal for applications where non-toxic solvents are required and controlled release of the polyphenol content is desired.
Eleni Spanidi; Athanasios Karapetsas; Georgia-Persephoni Voulgaridou; Sophia Letsiou; Nektarios Aligiannis; Ilias Tsochantaridis; Spyridon Kynigopoulos; Maria Lambropoulou; Ioannis Mourtzinos; Aglaia Pappa; Konstantinos Gardikis. A New Controlled Release System for Propolis Polyphenols and Its Biochemical Activity for Skin Applications. Plants 2021, 10, 420 .
AMA StyleEleni Spanidi, Athanasios Karapetsas, Georgia-Persephoni Voulgaridou, Sophia Letsiou, Nektarios Aligiannis, Ilias Tsochantaridis, Spyridon Kynigopoulos, Maria Lambropoulou, Ioannis Mourtzinos, Aglaia Pappa, Konstantinos Gardikis. A New Controlled Release System for Propolis Polyphenols and Its Biochemical Activity for Skin Applications. Plants. 2021; 10 (2):420.
Chicago/Turabian StyleEleni Spanidi; Athanasios Karapetsas; Georgia-Persephoni Voulgaridou; Sophia Letsiou; Nektarios Aligiannis; Ilias Tsochantaridis; Spyridon Kynigopoulos; Maria Lambropoulou; Ioannis Mourtzinos; Aglaia Pappa; Konstantinos Gardikis. 2021. "A New Controlled Release System for Propolis Polyphenols and Its Biochemical Activity for Skin Applications." Plants 10, no. 2: 420.
In the present study, we aimed to examine the antioxidant, antiaging and photoprotective properties of Greek honey samples of various botanical and geographical origin. Ethyl-acetate extracts were used and the and the total phenolic/flavonoid content and antioxidant capacity were evaluated. Honey extracts were then studied for their cytoprotective properties against UVB-induced photodamage using human immortalized keratinocytes (HaCaT) and/or reconstituted human skin tissue models. Specifically, the cytotoxicity, oxidative status, DNA damage and gene expression levels of specific matrix metalloproteinases (MMPs) were examined. Overall, the treatment of HaCaT cells with honey extracts resulted in lower levels of DNA strand breaks and attenuated the decrease in cell viability following UVB exposure. Additionally, honey extracts significantly decreased the total protein carbonyl content of the irradiated cells, however, they had no significant effect on their total antioxidant status. Finally, the extracts alleviated the UVB-induced up-regulation of MMPs-3, -7 and -9 in a model of reconstituted skin tissue. In conclusion, honey extracts exhibited significant photoprotective and antiaging properties under UVB exposure conditions and thus could be further exploited as promising agents for developing novel and naturally-based, antiaging cosmeceutical products.
Athanasios Karapetsas; Georgia-Persephoni Voulgaridou; Dimitra Iliadi; Ilias Tsochantaridis; Panagiota Michail; Spyridon Kynigopoulos; Maria Lambropoulou; Maria-Ioanna Stavropoulou; Konstantina Stathopoulou; Sofia Karabournioti; Nektarios Aligiannis; Konstantinos Gardikis; Alex Galanis; Mihalis I. Panayiotidis; Aglaia Pappa. Honey Extracts Exhibit Cytoprotective Properties against UVB-Induced Photodamage in Human Experimental Skin Models. Antioxidants 2020, 9, 566 .
AMA StyleAthanasios Karapetsas, Georgia-Persephoni Voulgaridou, Dimitra Iliadi, Ilias Tsochantaridis, Panagiota Michail, Spyridon Kynigopoulos, Maria Lambropoulou, Maria-Ioanna Stavropoulou, Konstantina Stathopoulou, Sofia Karabournioti, Nektarios Aligiannis, Konstantinos Gardikis, Alex Galanis, Mihalis I. Panayiotidis, Aglaia Pappa. Honey Extracts Exhibit Cytoprotective Properties against UVB-Induced Photodamage in Human Experimental Skin Models. Antioxidants. 2020; 9 (7):566.
Chicago/Turabian StyleAthanasios Karapetsas; Georgia-Persephoni Voulgaridou; Dimitra Iliadi; Ilias Tsochantaridis; Panagiota Michail; Spyridon Kynigopoulos; Maria Lambropoulou; Maria-Ioanna Stavropoulou; Konstantina Stathopoulou; Sofia Karabournioti; Nektarios Aligiannis; Konstantinos Gardikis; Alex Galanis; Mihalis I. Panayiotidis; Aglaia Pappa. 2020. "Honey Extracts Exhibit Cytoprotective Properties against UVB-Induced Photodamage in Human Experimental Skin Models." Antioxidants 9, no. 7: 566.
The aim of this study was to assess the antioxidant, photoprotective, and antiaging effects of Greek propolis. Propolis was subjected to n-heptane or methanol extraction. Total phenolic/flavonoid content and antioxidant potential were determined in the extracts. Promising extracts were evaluated for their cytoprotective properties using human immortalized keratinocyte (HaCaT) or reconstituted human skin tissue following exposure to UVB. Assessment of cytotoxicity, DNA damage, oxidative status, and gene/protein expression levels of various matrix metalloproteinases (MMPs) were performed. The propolis methanolic fractions exhibited higher total phenolic and flavonoid contents and significant in vitro antioxidant activity. Incubation of HaCaT cells with certain methanolic extracts significantly decreased the formation of DNA strand breaks following exposure to UVB and attenuated UVB-induced decrease in cell viability. The extracts had no remarkable effect on the total antioxidant status, but significantly lowered total protein carbonyl content used as a marker for protein oxidation in HaCaT cells. MMP-1, -3, -7, and -9, monitored as endpoints of antiaging efficacy, were significantly reduced by propolis following UVB exposure in a model of reconstituted skin tissue. In conclusion, propolis protects against the oxidative and photodamaging effects of UVB and could be further explored as a promising agent for developing natural antiaging strategies.
Athanasios Karapetsas; Georgia-Persephoni Voulgaridou; Manolis Konialis; Ilias Tsochantaridis; Spyridon Kynigopoulos; Maria Lambropoulou; Maria-Ioanna Stavropoulou; Konstantina Stathopoulou; Nektarios Aligiannis; Petros Bozidis; Anna Goussia; Konstantinos Gardikis; Mihalis I. Panayiotidis; Aglaia Pappa. Propolis Extracts Inhibit UV-Induced Photodamage in Human Experimental In Vitro Skin Models. Antioxidants 2019, 8, 125 .
AMA StyleAthanasios Karapetsas, Georgia-Persephoni Voulgaridou, Manolis Konialis, Ilias Tsochantaridis, Spyridon Kynigopoulos, Maria Lambropoulou, Maria-Ioanna Stavropoulou, Konstantina Stathopoulou, Nektarios Aligiannis, Petros Bozidis, Anna Goussia, Konstantinos Gardikis, Mihalis I. Panayiotidis, Aglaia Pappa. Propolis Extracts Inhibit UV-Induced Photodamage in Human Experimental In Vitro Skin Models. Antioxidants. 2019; 8 (5):125.
Chicago/Turabian StyleAthanasios Karapetsas; Georgia-Persephoni Voulgaridou; Manolis Konialis; Ilias Tsochantaridis; Spyridon Kynigopoulos; Maria Lambropoulou; Maria-Ioanna Stavropoulou; Konstantina Stathopoulou; Nektarios Aligiannis; Petros Bozidis; Anna Goussia; Konstantinos Gardikis; Mihalis I. Panayiotidis; Aglaia Pappa. 2019. "Propolis Extracts Inhibit UV-Induced Photodamage in Human Experimental In Vitro Skin Models." Antioxidants 8, no. 5: 125.
Despoina D. Kakagia; Alexandra Tsaroucha; Maria Lambropoulou. A Bowel of Flowers. International Journal of Surgical Pathology 2018, 27, 192 -192.
AMA StyleDespoina D. Kakagia, Alexandra Tsaroucha, Maria Lambropoulou. A Bowel of Flowers. International Journal of Surgical Pathology. 2018; 27 (2):192-192.
Chicago/Turabian StyleDespoina D. Kakagia; Alexandra Tsaroucha; Maria Lambropoulou. 2018. "A Bowel of Flowers." International Journal of Surgical Pathology 27, no. 2: 192-192.
BackgroundThe aim of the present study was to microscopically assess the tissue-sparing potential of contemporary radiofrequency-assisted liver resection (RF-LR) techniques.MethodsTwenty-four pigs were subjected to either (1) partial hepatectomy (PH) using the sequential-coagulate-cut (SCC) technique (group SCC, n = 6) using a monopolar electrode, the technique using the bipolar electrode Habib-4X (group H, n = 6) or the “crush-clamp” technique (group CC, n = 6); or (2) sham operation (group Sham, n = 6). At 48 h post-operation, liver parenchyma proximal to the ablation rim was excised for histopathologic examination and immunohistochemical assessment of apoptosis (antibody M30) and inflammatory response (antibodies IL-6, TNFα and NFκB).ResultsHistopathologic index increased from the 1st to the 4th, the 1st to the 2nd or only the 1st cm from the inner margin of the ablation rim in group SCC, H or CC, respectively. The index was higher in group SCC compared to the other groups. Tissue expression of M30, IL-6, TNFα and NFκB increased in all PH groups, being higher and more expanded in group SCC, H, SCC and SCC, respectively.ConclusionsRF-LR techniques had variable microscopically assessed tissue-sparing effect. The Habib-4X proved to be less injurious compared to the SCC Belgrade technique regarding the severity and extent of tissue damage proximal to the ablation rim.
Petros Ypsilantis; Maria Lambropoulou; Miroslav Milicevic; Predrag Bulajic; Anastasios Karayiannakis; Dimitrios Zacharoulis; Constantinos Simopoulos. Microscopic assessment of the tissue-sparing potential of radiofrequency-assisted liver resection techniques in a porcine model. Journal of Hepato-Biliary-Pancreatic Sciences 2017, 24, 657 -666.
AMA StylePetros Ypsilantis, Maria Lambropoulou, Miroslav Milicevic, Predrag Bulajic, Anastasios Karayiannakis, Dimitrios Zacharoulis, Constantinos Simopoulos. Microscopic assessment of the tissue-sparing potential of radiofrequency-assisted liver resection techniques in a porcine model. Journal of Hepato-Biliary-Pancreatic Sciences. 2017; 24 (12):657-666.
Chicago/Turabian StylePetros Ypsilantis; Maria Lambropoulou; Miroslav Milicevic; Predrag Bulajic; Anastasios Karayiannakis; Dimitrios Zacharoulis; Constantinos Simopoulos. 2017. "Microscopic assessment of the tissue-sparing potential of radiofrequency-assisted liver resection techniques in a porcine model." Journal of Hepato-Biliary-Pancreatic Sciences 24, no. 12: 657-666.
Background and Aim. Extended liver radiofrequency ablation (RFA) has been shown to disrupt gut barrier integrity with subsequent bacterial translocation. The aim of the present project was to study the immune and inflammatory responses of the intestinal mucosa after extended RFA of the liver.Methods. Twelve Wistar rats were either subjected to RFA of the left lateral hepatic lobe (approximately 30% of the liver mass) after midline laparotomy (group RFA,n=6) or sham operation (group Sham,n=6). Forty-eight hours later, ileal tissue specimens were excised for immunohistochemical assessment of CD68+macrophages, CD4+T-lymphocytes, CD8+T-lymphocytes, mucosal addressin cell adhesion molecule-1 (MAdCAM-1), tumor necrosis factor-α(TNFα), interleukin-6 (IL-6), and nuclear factor-κB (NFκB) expression.Results. Immune response biomarkers were upregulated in the RFA group. Expression of CD4+and CD8+T-lymphocytes was moderate, while that of CD68+macrophages and MAdCAM-1 was high. Inflammatory response biomarkers were also upregulated in the RFA group. TNFα, IL-6, and NFκB expression was low, moderate, and high, respectively.Conclusions. Extended liver RFA evokes both immune and inflammatory responses of the gut mucosa.
Petros Ypsilantis; Maria Lambropoulou; Antonios Evagellou; Nikolaos Papadopoulos; Constantinos Simopoulos. Immune and Inflammatory Responses of the Intestinal Mucosa following Extended Liver Radiofrequency Ablation. Gastroenterology Research and Practice 2017, 2017, 1 -6.
AMA StylePetros Ypsilantis, Maria Lambropoulou, Antonios Evagellou, Nikolaos Papadopoulos, Constantinos Simopoulos. Immune and Inflammatory Responses of the Intestinal Mucosa following Extended Liver Radiofrequency Ablation. Gastroenterology Research and Practice. 2017; 2017 ():1-6.
Chicago/Turabian StylePetros Ypsilantis; Maria Lambropoulou; Antonios Evagellou; Nikolaos Papadopoulos; Constantinos Simopoulos. 2017. "Immune and Inflammatory Responses of the Intestinal Mucosa following Extended Liver Radiofrequency Ablation." Gastroenterology Research and Practice 2017, no. : 1-6.
Purpose: We investigated the hepatoprotective effect of Silibinin (SLB) to ischemia-reperfusion (I/R) rat model, by evaluating the histological expression of the tissue markers Fas/FasL, HMGB-1 and CD45, and SLB pharmacokinetics. Methods: Seventy-three Wistar-type male rats were randomized in 11 groups: Sham control group (open-close laparotomy); four I/R control groups (laparotomy, 45 min vascular occlusion, reperfusion, euthanasia after 60, 120, 180, and 240 min); four SLB (Si) groups (laparotomy, 45 min vascular occlusion, IV administration of SLB, reperfusion, euthanasia after 60, 120, 180, and 240 min); two SLB pharmacokinetics (PK) groups (IV administration of SLB, euthanasia after 45 and 240 min). Results: Fas/FasL increased with reperfusion time in I/R control groups and decreased in the Si groups, reaching, respectively, the highest and lowest values at 240 min of reperfusion (p <.0001). HMGB1 and CD45 increased with time in the I/R control groups up to 240 min and decreased in the Si groups, approaching zero expression after 180 and 60 min, respectively. Pharmacokinetic data showed higher liver accumulation and slower plasma elimination of SLB in ischemic animals. Conclusions: The hepatoprotective effect of SLB was demonstrated through the reduction of the expression of Fas/FasL, HMGB-1 and CD45 in liver tissue under I/R conditions, and in the pharmacokinetic study. The results document the efficacy of silibinin in the protection of the liver, and are particularly encouraging for its use in hepatic surgery.
Alexandra K. Tsaroucha; Georgia Valsami; Nikolaos Kostomitsopoulos; Maria Lambropoulou; Constantinos Anagnostopoulos; Eirini Christodoulou; Evangelos Falidas; Afrodite Betsou; Michael Pitiakoudis; Constantinos E. Simopoulos. Silibinin Effect on Fas/FasL, HMGB1, and CD45 Expressions in a Rat Model Subjected to Liver Ischemia-Reperfusion Injury. Journal of Investigative Surgery 2017, 31, 491 -502.
AMA StyleAlexandra K. Tsaroucha, Georgia Valsami, Nikolaos Kostomitsopoulos, Maria Lambropoulou, Constantinos Anagnostopoulos, Eirini Christodoulou, Evangelos Falidas, Afrodite Betsou, Michael Pitiakoudis, Constantinos E. Simopoulos. Silibinin Effect on Fas/FasL, HMGB1, and CD45 Expressions in a Rat Model Subjected to Liver Ischemia-Reperfusion Injury. Journal of Investigative Surgery. 2017; 31 (6):491-502.
Chicago/Turabian StyleAlexandra K. Tsaroucha; Georgia Valsami; Nikolaos Kostomitsopoulos; Maria Lambropoulou; Constantinos Anagnostopoulos; Eirini Christodoulou; Evangelos Falidas; Afrodite Betsou; Michael Pitiakoudis; Constantinos E. Simopoulos. 2017. "Silibinin Effect on Fas/FasL, HMGB1, and CD45 Expressions in a Rat Model Subjected to Liver Ischemia-Reperfusion Injury." Journal of Investigative Surgery 31, no. 6: 491-502.
Background: Hepatocellular carcinoma (HCC) is one of the most common malignant tumors. Despite the important progress observed in liver surgery, the survival rates are discouraging. The aim of this study was to investigate the expression of autotaxin in hepatocellular carcinoma. Materials and Methods: Liver tissues from 28 human hepatocellular carcinomas were evaluated for the expression of autotaxin by immunohistochemistry. The gender, age, histological grade, lymphovascular invasion, number of tumors, levels of serum alpha-fetoprotein (aFP), presence of liver cirrhosis, hepatitis, surgery and survival rates were recorded. Results: Immunohistochemistry confirmed the expression of autotaxin in hepatocellular carcinoma. The histological grade seems to be the only independent predictor of stronger autotaxin expression, as significantly higher levels of autotaxin were detected in histological grades II and III. In addition, levels of autotaxin seem to be the most important independent prognostic factor related to poor survival. There was an eight-fold higher risk of death in patients with high levels of autotaxin compared to patients with low levels. Conclusions: Autotaxin expression in hepatocellular carcinoma could be of great importance. High autotaxin expression in HCC is detected in patients with histological grade II and III. Further, patients with elevated expression levels were found to possess an eight-fold higher risk of death. Autotaxin role in HCC should be further elucidated.
Ilker Memet; Evanthia Tsalkidou; Alexandra K Tsaroucha; Maria Lambropoulou; Ekaterini Chatzaki; Gregory Trypsianis; Dimitrios Schizas; Michael Pitiakoudis; Konstantinos Simopoulos. Autotaxin Expression in Hepatocellular Carcinoma. Journal of Investigative Surgery 2017, 31, 359 -365.
AMA StyleIlker Memet, Evanthia Tsalkidou, Alexandra K Tsaroucha, Maria Lambropoulou, Ekaterini Chatzaki, Gregory Trypsianis, Dimitrios Schizas, Michael Pitiakoudis, Konstantinos Simopoulos. Autotaxin Expression in Hepatocellular Carcinoma. Journal of Investigative Surgery. 2017; 31 (5):359-365.
Chicago/Turabian StyleIlker Memet; Evanthia Tsalkidou; Alexandra K Tsaroucha; Maria Lambropoulou; Ekaterini Chatzaki; Gregory Trypsianis; Dimitrios Schizas; Michael Pitiakoudis; Konstantinos Simopoulos. 2017. "Autotaxin Expression in Hepatocellular Carcinoma." Journal of Investigative Surgery 31, no. 5: 359-365.
Art and Science are usually considered fields of different perceptions and approaches with very little overlap, but when combined, sometimes something unusually beautiful and unexpected can arise. Currently, a major effort is being made to promote the interaction between artists and scientists as demonstrated by some of the most recent grant programmes by organizations such as the United Kingdom’s Engineering and Physical Sciences Research Council (EPSRC). Occasionally, artists are approaching science with slightly different insights than those expected, but to date, there is a gap that is rarely violated despite the extraordinary ideas that may arise from such collaborations. There can be no doubt that Nature is the greatest inspiration for art and beauty. In many ways, science attempts to reveal the rules and the beauty of nature. Microscopy, as a technique in scientific research, plays a key role in revealing the beauty of nature, a trend that has led some to regard it as a form of art. But can this claim be justified? For the uninitiated, microscopy could provide a high-resolution image of nature’s creations, revealing at the same time its inherent beauty. The cells – human or non-human – form images of the same beauty as those represented in paintings. Preparations observed under the microscope contain a wealth of information. The aim of the researcher is to correlate the cellular structure with the functioning of the organism and ultimately with the miracle of life. The aesthetic quality of the images has led many scientists to stand in their artistic nature. But can we say that microscopy, which is essentially a way of acquiring and analyzing scientific data, is a form of art? If art is the creation of works by people of extraordinary talent, then microscopy might be an art form and a technique that seeks the functional disclosure of complex admirers of cellular structures. However, unlike music or painting, microscopy is certainly not a form of art, with the traditional concept of creativity. The question therefore arises as to whether the aesthetic nature of the images revealed by a microscope can be considered as art. This is a question that is the starting point of this article.
Antigoni Avramouli; Maria Gonidi; Maria Lambropoulou. Microscopy as a form of art. Technoetic Arts 2017, 15, 195 -202.
AMA StyleAntigoni Avramouli, Maria Gonidi, Maria Lambropoulou. Microscopy as a form of art. Technoetic Arts. 2017; 15 (2):195-202.
Chicago/Turabian StyleAntigoni Avramouli; Maria Gonidi; Maria Lambropoulou. 2017. "Microscopy as a form of art." Technoetic Arts 15, no. 2: 195-202.
Different studies have suggested that the expression of biomarkers related to lymphoid cell activation may provide information on the behavior of DLBCL. Most studies have concentrated on nodal or a mixture of nodal and extranodal lymphomas. The differential expression and potential clinical impact of these markers in a homogeneous group of extranodal DLBCLs are not well defined. In this study, we investigated the expression of three activation markers, Blimp1, Foxp1 and pStat3, in a cohort of 35 extranodal DLBCLs homogeneously treated with R-CHOP. Immunohistochemical stains were evaluated using an immunoreactivity score on representative paraffin sections. Blimp1 was positive in 55% (19/35), Foxp1 in 60% (21/35), and pStat3 in 69% (24/35) of our cases. We did not observe any statistical differences in the expression of these markers in GCB and non-GCB tumors or in gastrointestinal and non-gastrointestinal tumors. Blimp1 expression was negatively correlated with overall survival (OS) (p=0.001) in the whole series and in the non-GCB group (Muris algorithm) (p=0.002). Foxp1 positivity and pStat3 positivity had no impact on the outcome of the patients in the global cohort, but they were associated with a better survival in the non-GCB subgroup (p=0.033, p=0.044 respectively). Multivariate analysis showed that Blimp1 expression but not COO was an independent negative prognostic factor for OS (HR=17.5, 95%, CI=2.2-141.1, p=0.007). Our results suggest that these markers are differentially expressed and have different impacts on outcome in extranodal DLBCLs compared to nodal tumors, emphasizing the need to evaluate separately these and probably other markers in these subsets of tumors.
Georgios Petrakis; Ioannis Kostopoulos; Ioannis Venizelos; Maria Lambropoulou; Kyriakos Vouras; Sofia Vakalopoulou; Eudokia Mandala; Constantinos Tsatalas; Nicolas Papadopoulos. Expression of the activation markers Blimp1, Foxp1 and pStat3 in extranodal diffuse large B-cell lymphomas. Histol. Histopathol. 2017, 32, 825 -834.
AMA StyleGeorgios Petrakis, Ioannis Kostopoulos, Ioannis Venizelos, Maria Lambropoulou, Kyriakos Vouras, Sofia Vakalopoulou, Eudokia Mandala, Constantinos Tsatalas, Nicolas Papadopoulos. Expression of the activation markers Blimp1, Foxp1 and pStat3 in extranodal diffuse large B-cell lymphomas. Histol. Histopathol.. 2017; 32 (32):825-834.
Chicago/Turabian StyleGeorgios Petrakis; Ioannis Kostopoulos; Ioannis Venizelos; Maria Lambropoulou; Kyriakos Vouras; Sofia Vakalopoulou; Eudokia Mandala; Constantinos Tsatalas; Nicolas Papadopoulos. 2017. "Expression of the activation markers Blimp1, Foxp1 and pStat3 in extranodal diffuse large B-cell lymphomas." Histol. Histopathol. 32, no. 32: 825-834.
Background: Remote kidney damage is a sequel of hepatic ischemia–reperfusion (I/R) injury. Silibinin is the main ingredient of the milk thistle plant seed extract with known antioxidant and hepatoprotective activity. Our study investigates the nephroprotective potential of intravenously administered silibinin, as a lyophilized SLB-hydoxypropyl-beta-cyclodextrin product, in hepatic I/R injury. Material and methods: 63 Wistar rats were divided into three groups: Sham (virtual intervention); Control (45 min ischemia and reperfusion); and Silibinin (200 μL intravenous silibinin administration after 45 min of ischemia). Kidney tissues were collected to determine TNF-α, M30 and histopathological changes at predetermined time intervals. Results: Comparing Sham vs. Control groups, proved that hepatic I/R injury increased renal TNF-α and M30 expression. Deterioration was observed in hyperemia/filtration of renal parenchyma and tubules, cortical filtration, tubular necrosis and edema (tissue swelling index). Intravenous silibinin administration and comparison of the Control vs. Silibinin groups showed a statistically significant decrease in TNF-α levels at 240 min following I/R (p < 0.0001), and in M30 at 180 min (p = 0.03) and 240 min (p < 0.0001). Renal parameters have significantly decreased in: hyperemia/filtration of renal parenchyma at 120 min (p = 0.003), 180 min (p = 0.0001) and 240 min (p = 0.0002); hyperemia/filtration of renal tubules at 120 min (p = 0.02), 180 min (p = 0.0001) and 240 min (p = 0.0005); cortical filtration (240 min - p = 0.005); tubular necrosis (240 min - p = 0.021); and edema (240 min - p = 0.001). Conclusion: Our study confirms that hepatic I/R injury causes remote renal damage while the intravenous administration of silibinin leads to statistically significant nephroprotective action.
Georgios Kyriakopoulos; Alexandra K. Tsaroucha; Georgia Valsami; Maria Lambropoulou; Nikolaos Kostomitsopoulos; Eirini Christodoulou; Zacharias Kakazanis; Constantinos Anagnostopoulos; Christos Tsalikidis; Constantinos E. Simopoulos. Silibinin Improves TNF-α and M30 Expression and Histological Parameters in Rat Kidneys After Hepatic Ischemia/Reperfusion. Journal of Investigative Surgery 2017, 31, 201 -209.
AMA StyleGeorgios Kyriakopoulos, Alexandra K. Tsaroucha, Georgia Valsami, Maria Lambropoulou, Nikolaos Kostomitsopoulos, Eirini Christodoulou, Zacharias Kakazanis, Constantinos Anagnostopoulos, Christos Tsalikidis, Constantinos E. Simopoulos. Silibinin Improves TNF-α and M30 Expression and Histological Parameters in Rat Kidneys After Hepatic Ischemia/Reperfusion. Journal of Investigative Surgery. 2017; 31 (3):201-209.
Chicago/Turabian StyleGeorgios Kyriakopoulos; Alexandra K. Tsaroucha; Georgia Valsami; Maria Lambropoulou; Nikolaos Kostomitsopoulos; Eirini Christodoulou; Zacharias Kakazanis; Constantinos Anagnostopoulos; Christos Tsalikidis; Constantinos E. Simopoulos. 2017. "Silibinin Improves TNF-α and M30 Expression and Histological Parameters in Rat Kidneys After Hepatic Ischemia/Reperfusion." Journal of Investigative Surgery 31, no. 3: 201-209.
Cervical cancer is strongly related to certain high-risk types of human papilloma virus infection. Breast cancer metastasis suppressor 1 (BRMS1) is a tumor suppressor gene, its expression being regulated by DNA promoter methylation in several types of cancers. This study aims to evaluate the methylation status of BRMS1 promoter in relation to high-risk types of human papilloma virus infection and the development of pre-cancerous lesions and describe the pattern of BRMS1 protein expression in normal, high-risk types of human papilloma virus–infected pre-cancerous and malignant cervical epithelium. We compared the methylation status of BRMS1 in cervical smears of 64 women with no infection by high-risk types of human papilloma virus to 70 women with proven high-risk types of human papilloma virus infection, using real-time methylation-specific polymerase chain reaction. The expression of BRMS1 protein was described by immunohistochemistry in biopsies from cervical cancer, pre-cancerous lesions, and normal cervices. Methylation of BRMS1 promoter was detected in 37.5% of women with no high-risk types of human papilloma virus infection and was less frequent in smears with high-risk types of human papilloma virus (11.4%) and in women with pathological histology (cervical intraepithelial neoplasia) (11.9%). Methylation was detected also in HeLa cervical cancer cells. Immunohistochemistry revealed nuclear BRMS1 protein staining in normal high-risk types of human papilloma virus–free cervix, in cervical intraepithelial neoplasias, and in malignant tissues, where staining was occasionally also cytoplasmic. In cancer, expression was stronger in the more differentiated cancer blasts. In conclusion, BRMS1 promoter methylation and aberrant protein expression seem to be related to high-risk types of human papilloma virus–induced carcinogenesis in uterine cervix and is worthy of further investigation.
Maria Panagopoulou; Maria Lambropoulou; Ioanna Balgkouranidou; Evangelia Nena; Makrina Karaglani; Christina Nicolaidou; Anthi Asimaki; Theocharis Konstantinidis; Theodoros C Constantinidis; George Kolios; Stylianos Kakolyris; Theodoros Agorastos; Ekaterini Chatzaki. Gene promoter methylation and protein expression of BRMS1 in uterine cervix in relation to high-risk human papilloma virus infection and cancer. Tumor Biology 2017, 39, 1 .
AMA StyleMaria Panagopoulou, Maria Lambropoulou, Ioanna Balgkouranidou, Evangelia Nena, Makrina Karaglani, Christina Nicolaidou, Anthi Asimaki, Theocharis Konstantinidis, Theodoros C Constantinidis, George Kolios, Stylianos Kakolyris, Theodoros Agorastos, Ekaterini Chatzaki. Gene promoter methylation and protein expression of BRMS1 in uterine cervix in relation to high-risk human papilloma virus infection and cancer. Tumor Biology. 2017; 39 (4):1.
Chicago/Turabian StyleMaria Panagopoulou; Maria Lambropoulou; Ioanna Balgkouranidou; Evangelia Nena; Makrina Karaglani; Christina Nicolaidou; Anthi Asimaki; Theocharis Konstantinidis; Theodoros C Constantinidis; George Kolios; Stylianos Kakolyris; Theodoros Agorastos; Ekaterini Chatzaki. 2017. "Gene promoter methylation and protein expression of BRMS1 in uterine cervix in relation to high-risk human papilloma virus infection and cancer." Tumor Biology 39, no. 4: 1.
Purpose/aim: To examine with immunohistochemical assay MTA1 protein expression levels in pancreatic cancer tissues defining its prognostic value. Material and Methods: The specimens derived from 51 patients who underwent surgery. The levels of MTA1 protein were compared with the age of the patients, their survival, and prognosis. Also, we studied clinical and histopathological factors such as the degree of tumor differentiation and its stage in correlation with MTA1 protein levels. In parallel, there was correlation between the expression of the ΜΤΑ1 protein and the aforementioned factors regarding survival rate. Furthermore, we independently correlated the patient's survival in relation to whether they had undergone adjuvant chemotherapy or not. Results: It has been found to be low, moderate, or high expression of MTA1 levels in 48 out of 51 cancer tissues. Specifically, 49.0% of patients had low expression, 33.3% moderate, and 11.8% high expression of MTA1. Regarding the expression of MTA1 protein in correlation with various clinical and histopathological factors, a statistically significant correlation was observed with the degree of differentiation (p = 0.0068) and with the stage of the disease (p = 0.0173), but not with survival (p = 0.0740) or the age of them (p = 0.1547). Finally, it was found that overexpression of the MTA1protein is a prognostic factor for shorter survival in patients with pancreatic cancer (average 4.67 ± 0.95 months). Conclusions: MTA 1 protein may constitute an important prognostic marker in pancreatic cancer and could improve prognosis and treatment.
Efstathios T. Pavlidis; Maria Lambropoulou; Nikolaos G. Symeonidis; Constantinos Anagnostopoulos; Alexandra Tsaroucha; Athanasia Kotini; Christina Nikolaidou; Anastasia Kiziridou; Konstantinos Simopoulos. The Immunohistochemical Expression MTA 1 Protein and its Prognostic Value in Pancreatic Cancer. Journal of Investigative Surgery 2017, 31, 142 -150.
AMA StyleEfstathios T. Pavlidis, Maria Lambropoulou, Nikolaos G. Symeonidis, Constantinos Anagnostopoulos, Alexandra Tsaroucha, Athanasia Kotini, Christina Nikolaidou, Anastasia Kiziridou, Konstantinos Simopoulos. The Immunohistochemical Expression MTA 1 Protein and its Prognostic Value in Pancreatic Cancer. Journal of Investigative Surgery. 2017; 31 (2):142-150.
Chicago/Turabian StyleEfstathios T. Pavlidis; Maria Lambropoulou; Nikolaos G. Symeonidis; Constantinos Anagnostopoulos; Alexandra Tsaroucha; Athanasia Kotini; Christina Nikolaidou; Anastasia Kiziridou; Konstantinos Simopoulos. 2017. "The Immunohistochemical Expression MTA 1 Protein and its Prognostic Value in Pancreatic Cancer." Journal of Investigative Surgery 31, no. 2: 142-150.
Background. Several investigators have suggested the possibility that the expression of both EGFR and HER2 could be utilized for molecularly targeted therapy in urinary bladder cancer. We tried to evaluate the expression of HER2 and EGFR and activation of the AKT/PTEN/mTOR pathway in urothelial carcinomas and if there is any association between them and cellular adhesion molecules (CAMs). Materials and Methods. Forty-one paraffin-embedded urothelial cancer tissue blocks were collected. Immunostains for HER2, EGFR, MIB1, phospho-AKT, PTEN, phospho-mTOR, e-cadherin, p-cadherin, and b-catenin were performed on tissue microarrays sections. The immunohistochemical results were correlated with clinicopathological parameters. Results. The overexpression of HER2 was found in 19.6% of the cases and it was associated with high grade tumors with a high mitotic index and phosphorylation of AKT and mTOR. Muscle-invasive tumors presented both cytoplasmic and nuclear losses of PTEN expression. There was no association between HER/AKT/mTOR pathway activation and CAM expression. Although cadherins were often coexpressed, only p-cadherin immunoreactivity was associated with tumor grade and high proliferative index. Conclusions. HER2 overexpression is found in a respective proportion of urothelial carcinomas. P-cadherin expression is associated with high grade UCs but it is not affected by HER2 overexpression or by activation of HER/AKT/mTOR pathway.
Nikolaos Koletsas; Triantafyllia Koletsa; Spyros Choidas; Konstantinos Anagnostopoulos; Stavros Touloupidis; Thomas Zaramboukas; Georgia Raptou; Nikolaos Papadopoulos; Maria Lambropoulou. Immunohistochemical Investigation of HER/AKT/mTOR Pathway and Cellular Adhesion Molecules in Urothelial Carcinomas. Pathology Research International 2017, 2017, 1 -7.
AMA StyleNikolaos Koletsas, Triantafyllia Koletsa, Spyros Choidas, Konstantinos Anagnostopoulos, Stavros Touloupidis, Thomas Zaramboukas, Georgia Raptou, Nikolaos Papadopoulos, Maria Lambropoulou. Immunohistochemical Investigation of HER/AKT/mTOR Pathway and Cellular Adhesion Molecules in Urothelial Carcinomas. Pathology Research International. 2017; 2017 ():1-7.
Chicago/Turabian StyleNikolaos Koletsas; Triantafyllia Koletsa; Spyros Choidas; Konstantinos Anagnostopoulos; Stavros Touloupidis; Thomas Zaramboukas; Georgia Raptou; Nikolaos Papadopoulos; Maria Lambropoulou. 2017. "Immunohistochemical Investigation of HER/AKT/mTOR Pathway and Cellular Adhesion Molecules in Urothelial Carcinomas." Pathology Research International 2017, no. : 1-7.
A Liiberis; Maria Lambropoulou; P Tsikouras; I Mylonas; G Trypsianis; N Papadopoulos; G Galazios. Immunohistochemical expression of MTA1 and MTA3 in placental tissue of normal and preeclamptic pregnancies. Clinical and Experimental Obstetrics & Gynecology 2017, 44, 370 -373.
AMA StyleA Liiberis, Maria Lambropoulou, P Tsikouras, I Mylonas, G Trypsianis, N Papadopoulos, G Galazios. Immunohistochemical expression of MTA1 and MTA3 in placental tissue of normal and preeclamptic pregnancies. Clinical and Experimental Obstetrics & Gynecology. 2017; 44 (3):370-373.
Chicago/Turabian StyleA Liiberis; Maria Lambropoulou; P Tsikouras; I Mylonas; G Trypsianis; N Papadopoulos; G Galazios. 2017. "Immunohistochemical expression of MTA1 and MTA3 in placental tissue of normal and preeclamptic pregnancies." Clinical and Experimental Obstetrics & Gynecology 44, no. 3: 370-373.
E Papadopoulos; C Nikolaidou; A Kotini; T E Deftereou; J Venizelos; P Pavlidis; N Gkantsinikoudis; N Papadopoulos; Maria Lambropoulou. A comparative immunohistochemical investigation of the consequences of chorioamnionitis on the developing human fetal spleen. Clinical and Experimental Obstetrics & Gynecology 2017, 44, 30 -38.
AMA StyleE Papadopoulos, C Nikolaidou, A Kotini, T E Deftereou, J Venizelos, P Pavlidis, N Gkantsinikoudis, N Papadopoulos, Maria Lambropoulou. A comparative immunohistochemical investigation of the consequences of chorioamnionitis on the developing human fetal spleen. Clinical and Experimental Obstetrics & Gynecology. 2017; 44 (1):30-38.
Chicago/Turabian StyleE Papadopoulos; C Nikolaidou; A Kotini; T E Deftereou; J Venizelos; P Pavlidis; N Gkantsinikoudis; N Papadopoulos; Maria Lambropoulou. 2017. "A comparative immunohistochemical investigation of the consequences of chorioamnionitis on the developing human fetal spleen." Clinical and Experimental Obstetrics & Gynecology 44, no. 1: 30-38.
Testicular torsion is an acute urologic emergency occurring in male newborns, children or adolescents. Prolonged ischemia for more than six hours can lead to irreversible testicular damage. Surgical detorsion allows reperfusion and is the only treatment currently available. The aim of this study was to evaluate the antioxidant effect of apigenin (APG) on the testicular ischemia-reperfusion (I/R) injury. Forty-two Wistar rats were randomly divided into five groups. Sham group underwent operation of the left testis. In the torsion-detorsion groups C15 and C120, the left testis was rotated 1080o for three hours. The treatment groups Ap15 and Ap120 received the same surgical procedure as groups C15 and C120, but APG was administered intravenously at the same time of detorsion via the right femoral vein. Left orchiectomy was performed 15 min after detorsion at groups C15 and Ap15, and at 120 min at groups C120 and Ap120 for histopathologic and immunohistochemical evaluation. In I/R-untreated groups C15 and C120, there was a moderate to severe distortion of the tubules with lesions that varied between grades III and IV according to histopathological finding. In APG-treated groups Ap15 and Ap120, most of the lesions showed injuries of grades II and III with mild and moderate histopathological features. In Terminal deoxynucleotide transferase dUTP Nick End Labeling (Tunel) assay, APG-treated animals showed a statistically significantly decreased number of apoptotic cells compared to groups C15 and C120. Intravenous administration of APG seems to have a protective effect on testicular ischemia-reperfusion injury after testicular torsion and detorsion. Hippokratia 2015; 19 (3): 225-230.
I Skondras; Maria Lambropoulou; A Tsaroucha; S Gardikis; G Tripsianis; C Simopoulos; G Vaos. The role of Apigenin in testicular damage in experimental ischemia-reperfusion injury in rats. Hippokratia 2016, 19, 225 -230.
AMA StyleI Skondras, Maria Lambropoulou, A Tsaroucha, S Gardikis, G Tripsianis, C Simopoulos, G Vaos. The role of Apigenin in testicular damage in experimental ischemia-reperfusion injury in rats. Hippokratia. 2016; 19 (3):225-230.
Chicago/Turabian StyleI Skondras; Maria Lambropoulou; A Tsaroucha; S Gardikis; G Tripsianis; C Simopoulos; G Vaos. 2016. "The role of Apigenin in testicular damage in experimental ischemia-reperfusion injury in rats." Hippokratia 19, no. 3: 225-230.
The incidence of heroin-induced myocardial infarction is rare, and the actual mechanism remains unclear. The increased heart weight in relation to the increased thickness of the heart walls may be an aggravating factor, and thus, it is a fact that should be investigated.
Pavlos Pavlidis; Theodora-Eleftheria Deftereou; Maria-Valeria Karakasi; Nikolaos Papadopoulos; Athanassios Zissimopoulos; Olga Pagonopoulou; Maria Lambropoulou. Intravenous Heroin Abuse and Acute Myocardial Infarction. American Journal of Forensic Medicine & Pathology 2016, 37, 95 -98.
AMA StylePavlos Pavlidis, Theodora-Eleftheria Deftereou, Maria-Valeria Karakasi, Nikolaos Papadopoulos, Athanassios Zissimopoulos, Olga Pagonopoulou, Maria Lambropoulou. Intravenous Heroin Abuse and Acute Myocardial Infarction. American Journal of Forensic Medicine & Pathology. 2016; 37 (2):95-98.
Chicago/Turabian StylePavlos Pavlidis; Theodora-Eleftheria Deftereou; Maria-Valeria Karakasi; Nikolaos Papadopoulos; Athanassios Zissimopoulos; Olga Pagonopoulou; Maria Lambropoulou. 2016. "Intravenous Heroin Abuse and Acute Myocardial Infarction." American Journal of Forensic Medicine & Pathology 37, no. 2: 95-98.
The corticotropin-releasing factor (CRF) family consists of the neuropeptides CRF, Ucn I, II and III and the binding sites CRFR1, CRFR2 and CRF-BP. It regulates stress response and the homeostasis of an organism. In this study, we examined the presence of the CRF system in the human hearts of normal and pathological fetuses. Heart tissues from 40 archival human fetuses were divided into Group A (without pathology, 'normal'), Group B (with chromosomal abnormalities) and Group C (with congenital disorders). Immunohistochemistry was used to localize the CRF system. Results correlated to gestational trimester and pathology. Immunoreactivity for all antigens was found in cardiac myocytes of all groups, in almost all samples, except Ucn III which was present in almost half of the fetuses of Groups B and C and was not detected at all in Group A. Ucn III was more often present during the earlier stage of development (<21weeks) and in fetuses with congenital disorders. In a fetus diagnosed with heart pathology, all but Ucn III antigens were also present. We localized a complete CRF system in the human fetal heart and correlated the presence of Ucn III to development and pathology. More studies are needed to verify and clarify the exact role of the CRF system in the human fetal heart.
Efterpi Chouridou; Maria Lambropoulou; Maria Koureta; Christina Zarouchlioti; Ioanna Balgouranidou; Evangelia Nena; Nikolaos Papadopoulos; Ekaterini Chatzaki. Corticotropin-releasing factor (CRF) system localization in human fetal heart. Hormones 2016, 15, 55 -64.
AMA StyleEfterpi Chouridou, Maria Lambropoulou, Maria Koureta, Christina Zarouchlioti, Ioanna Balgouranidou, Evangelia Nena, Nikolaos Papadopoulos, Ekaterini Chatzaki. Corticotropin-releasing factor (CRF) system localization in human fetal heart. Hormones. 2016; 15 (1):55-64.
Chicago/Turabian StyleEfterpi Chouridou; Maria Lambropoulou; Maria Koureta; Christina Zarouchlioti; Ioanna Balgouranidou; Evangelia Nena; Nikolaos Papadopoulos; Ekaterini Chatzaki. 2016. "Corticotropin-releasing factor (CRF) system localization in human fetal heart." Hormones 15, no. 1: 55-64.
Aim. Acute pancreatitis is an inflammatory intra-abdominal disease, which takes a severe form in 15–20% of patients and can result in high mortality especially when complicated by acute renal failure. The aim of this study is to assess the possible reduction in the extent of acute kidney injury after administration of eugenol in an experimental model of acute pancreatitis. Materials and Methods. 106 male Wistar rats weighing 220–350 g were divided into 3 groups: (1) Sham, with sham surgery; (2) Control, with induction of acute pancreatitis, through ligation of the biliopancreatic duct; and (3) Eugenol, with induction of acute pancreatitis and eugenol administration at a dose of 15 mg/kg. Serum urea and creatinine, histopathological changes, TNF-α, IL-6, and MPO activity in the kidneys were evaluated at predetermined time intervals. Results. The group that was administered eugenol showed milder histopathological changes than the Control group, TNF-α activity was milder in the Eugenol group, and there was no difference in activity for MPO and IL-6. Serum urea and creatinine levels were lower in the Eugenol group than in the Control group. Conclusions. Eugenol administration was protective for the kidneys in an experimental model of acute pancreatitis in rats.
Charalampos Markakis; Alexandra Tsaroucha; Apostolos E. Papalois; Maria Lambropoulou; Eleftherios Spartalis; Christina Tsigalou; Konstantinos Romanidis; Konstantinos Simopoulos. The Role of Eugenol in the Prevention of Acute Pancreatitis-Induced Acute Kidney Injury: Experimental Study. HPB Surgery 2016, 2016, 1 -9.
AMA StyleCharalampos Markakis, Alexandra Tsaroucha, Apostolos E. Papalois, Maria Lambropoulou, Eleftherios Spartalis, Christina Tsigalou, Konstantinos Romanidis, Konstantinos Simopoulos. The Role of Eugenol in the Prevention of Acute Pancreatitis-Induced Acute Kidney Injury: Experimental Study. HPB Surgery. 2016; 2016 ():1-9.
Chicago/Turabian StyleCharalampos Markakis; Alexandra Tsaroucha; Apostolos E. Papalois; Maria Lambropoulou; Eleftherios Spartalis; Christina Tsigalou; Konstantinos Romanidis; Konstantinos Simopoulos. 2016. "The Role of Eugenol in the Prevention of Acute Pancreatitis-Induced Acute Kidney Injury: Experimental Study." HPB Surgery 2016, no. : 1-9.