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Lignin is a biodegradable, non-toxic, and renewable biopolymer. The present investigation focuses on the bio-fabrication of superparamagnetic iron oxide nanoparticles (Li-SPIONs) using lignin from agro-wastes (straw of paddy and wheat) as a capping and reducing agent. The main motive of this study is to produce the biopolymer mediated nanomaterials using lignin. It is very important and needed in medicinal field as biodegradable nano drug delivery vehicles. Lignin mediated biogenic iron oxide nanomaterials were characterized by various techniques. An analysis of Field Emission Scanning Microscopy and High-Resolution Transmission Electron Microscope reveals that spherical-shaped Lignin mediated biogenic iron oxide nanomaterials were 20 ± 2 nm and 32 ± 4 nm in size respectively. The vibrating-sample magnetometer analysis illustrates that the bio-synthesized nanoparticles contain saturation magnetization. The lignin and their functional groups are act as capping and reducing/stabilizing agent for formation of biogenic iron oxide nanomaterials with magnetic power. In addition, this study was to explore Lignin mediated biogenic iron oxide nanomaterials for antioxidant activities using 2,2-diphenyl-1-picrylhydrazyl assay. The IC50 values of the DPPH assay were as follows, 60 μg/mL (PLi-SPIONs) and 62 μg/mL (WLi-SPIONs). The rate of percent free radical inhibition was 59.85% determine its maximum antioxidant potential. Biogenic iron oxide nanomaterials were able to deactivate the free radicals and oxidative metabolites. It clearly shows that biogenic iron oxide nanomaterials have good magnetic and antioxidant properties, which could be used to produce and formulate novel drug and biomedical applications.
Rajiv Periakaruppan; Jianjie Li; Huiling Mei; Ying Yu; Shunkai Hu; Xuan Chen; Xinghui Li; Guiyi Guo. Agro-waste mediated biopolymer for production of biogenic nano iron oxide with superparamagnetic power and antioxidant strength. Journal of Cleaner Production 2021, 311, 127512 .
AMA StyleRajiv Periakaruppan, Jianjie Li, Huiling Mei, Ying Yu, Shunkai Hu, Xuan Chen, Xinghui Li, Guiyi Guo. Agro-waste mediated biopolymer for production of biogenic nano iron oxide with superparamagnetic power and antioxidant strength. Journal of Cleaner Production. 2021; 311 ():127512.
Chicago/Turabian StyleRajiv Periakaruppan; Jianjie Li; Huiling Mei; Ying Yu; Shunkai Hu; Xuan Chen; Xinghui Li; Guiyi Guo. 2021. "Agro-waste mediated biopolymer for production of biogenic nano iron oxide with superparamagnetic power and antioxidant strength." Journal of Cleaner Production 311, no. : 127512.
(1) Aims: This study was aimed to investigate the effects of organic and inorganic fertilizer application on the soil nutrients and microbiota in tea garden soil. (2) Method: Illumina Hiseq sequencing technique was conducted to analyze the microbial diversity and density in different fertilizer-applied tea garden soil. (3) Results: The results showed that Acidobacteria, Proteobacteria and Actinobacteria were the predominant bacterial species observed in the tea garden soil. Besides, the relative abundance of Basidiomycota, Ascomycota and Zygomycota fungal species were higher in the tea garden soil. Correlation analysis revealed that Acidibacter and Acidothermus were significantly correlated with chemical properties (such as total organic carbon (TOC), total phosphorus (TP) and available phosphorus (AP) contents) of the tea garden soil. Furthermore, all these microbes were abundant in medium rapeseed cake (MRSC) + green manure (GM) treated tea garden soil. (4) Conclusion: Based on the obtained results, we conclude that the application of MRSC + GM could be a preferred fertilizer to increase the soil nutrients (TOC, TP and AP content) and microbial population in the tea garden soil.
Haiping Fu; Huan Li; Peng Yin; Huiling Mei; Jianjie Li; Pinqian Zhou; Yuanjiang Wang; Qingping Ma; Anburaj Jeyaraj; Kuberan Thangaraj; Xuan Chen; Xinghui Li; Guiyi Guo. Integrated Application of Rapeseed Cake and Green Manure Enhances Soil Nutrients and Microbial Communities in Tea Garden Soil. Sustainability 2021, 13, 2967 .
AMA StyleHaiping Fu, Huan Li, Peng Yin, Huiling Mei, Jianjie Li, Pinqian Zhou, Yuanjiang Wang, Qingping Ma, Anburaj Jeyaraj, Kuberan Thangaraj, Xuan Chen, Xinghui Li, Guiyi Guo. Integrated Application of Rapeseed Cake and Green Manure Enhances Soil Nutrients and Microbial Communities in Tea Garden Soil. Sustainability. 2021; 13 (5):2967.
Chicago/Turabian StyleHaiping Fu; Huan Li; Peng Yin; Huiling Mei; Jianjie Li; Pinqian Zhou; Yuanjiang Wang; Qingping Ma; Anburaj Jeyaraj; Kuberan Thangaraj; Xuan Chen; Xinghui Li; Guiyi Guo. 2021. "Integrated Application of Rapeseed Cake and Green Manure Enhances Soil Nutrients and Microbial Communities in Tea Garden Soil." Sustainability 13, no. 5: 2967.
Anthracnose, caused by Colletotrichum gloeosporioides is one of the most serious diseases of tea plant [Camellia sinensis (L.) O. Kuntze]. MicroRNAs are key modulators of gene expression in defense responses and plant immunity; although, foliar application of exogenous caffeine in anthracnose disease control management has proven to be effective, miRNA-mediated regulatory mechanisms underlying caffeine-induced plant defense response to C. gloeosporioides remain unexplored in tea plant. Using high-throughput-sequencing, 24 miRNA sequencing data sets and 8 degradome data sets were generated from the susceptible cultivar Longjing43 (LJ43) and the resistant cultivar Zhongcha108 (ZC108) leaves treated with CK (Water), C. gloeosporioides-inoculation (CgI), exogenous caffeine (CN) and CgI + CN. Using sRNA sequencing, 424 conserved miRNAs and 417 novel miRNAs were identified; of these, 146 and 130 miRNAs were differentially expressed under CgI + CN treatment in the LJ43 and ZC108, respectively. Degradome sequencing identified 599 targets predicted to be cleaved by 210 conserved and 70 novel miRNAs. Majority of the annotated targets were found to involve in regulation of transcription factors, oxidation-reduction and metabolic process for plant growth and development as well as stress responses in tea plant against C. gloeosporioides stress. The expression pattern of eight miRNAs and their targets were validated by qRT-PCR, and correlation analysis of csn-miR164a_R+1_1ss21AG/NAC-17 and csn-miR396b-5p/GRF-1 showed highly significant negative R-value at 7th dpi under CgI + CN in the LJ43. This study provides important insights into the novel approach of exogenous caffeine-induced miRNAs dynamically exerts its fungicidal activity through regulating JA/ET signaling pathway, thereby accurately switch on LJ43 susceptibility nature to resistance activity against C. gloeosporioides infection.
Anburaj Jeyaraj; Tamilselvi Elango; Ying Yu; Xuefei Chen; Zhongwei Zou; Zhaotang Ding; Zhen Zhao; Xuan Chen; Xinghui Li; Linbo Chen. Impact of exogenous caffeine on regulatory networks of microRNAs in response to Colletotrichum gloeosporioides in tea plant. Scientia Horticulturae 2021, 279, 109914 .
AMA StyleAnburaj Jeyaraj, Tamilselvi Elango, Ying Yu, Xuefei Chen, Zhongwei Zou, Zhaotang Ding, Zhen Zhao, Xuan Chen, Xinghui Li, Linbo Chen. Impact of exogenous caffeine on regulatory networks of microRNAs in response to Colletotrichum gloeosporioides in tea plant. Scientia Horticulturae. 2021; 279 ():109914.
Chicago/Turabian StyleAnburaj Jeyaraj; Tamilselvi Elango; Ying Yu; Xuefei Chen; Zhongwei Zou; Zhaotang Ding; Zhen Zhao; Xuan Chen; Xinghui Li; Linbo Chen. 2021. "Impact of exogenous caffeine on regulatory networks of microRNAs in response to Colletotrichum gloeosporioides in tea plant." Scientia Horticulturae 279, no. : 109914.
An eco-friendly, low toxic and facile synthetic approach was employed to produce superparamagnetic biogenic iron oxide nanoparticles (SPBIONs) using an aqueous extract of tea-pruning waste as a reducing agent under alkaline conditions. As-biosynthesized SPBIONs were extensively characterized by various analytical tools. For instance, XRD pattern showed crystallinity and FTIR spectra revealed the presence of bioactive molecules required for the reduction of iron oxide ions. AFM and FESEM-EDX images showed agglomerated spherical shape with strong signals of iron metallic ions. The average crystallite sizes of SPBIONs were found to be 20 – 35 nm by HRTEM analysis. Zeta potential analysis confirmed that the surface charge of the green synthesized SPBIONs was highly negative (-25.2 mV) and stable. TGA curve reported a weight loss of 14 %, which occurred in SPBIONs over temperatures ranging from 50 °C to 950 °C due to the removal of water molecules and volatile compounds. VSM analysis revealed that SPBIONs exhibited superparamagnetic properties with a high-saturation magnetization value of 11 emu/g. In addition, the antioxidant property of SPBIONs was investigated with 1,1-Diphenyl-2-picrylhydrazyl (DPPH), a free radical assay, and it was seen that 70 μg/mL (IC50) of SPBIONs was able to neutralize the generation of free radicals and oxidative stress. The present study successfully demonstrated that utilization of tea resources for the production of SPBIONs with superparamagnetic and antioxidant properties might be used to design antioxidant agents and other biomedical applications.
Rajiv Periakaruppan; Xuan Chen; Kuberan Thangaraj; Anburaj Jeyaraj; Hoang Ha Nguyen; Ying Yu; Shunkai Hu; Li Lu; Xinghui Li. Utilization of tea resources with the production of superparamagnetic biogenic iron oxide nanoparticles and an assessment of their antioxidant activities. Journal of Cleaner Production 2020, 278, 123962 .
AMA StyleRajiv Periakaruppan, Xuan Chen, Kuberan Thangaraj, Anburaj Jeyaraj, Hoang Ha Nguyen, Ying Yu, Shunkai Hu, Li Lu, Xinghui Li. Utilization of tea resources with the production of superparamagnetic biogenic iron oxide nanoparticles and an assessment of their antioxidant activities. Journal of Cleaner Production. 2020; 278 ():123962.
Chicago/Turabian StyleRajiv Periakaruppan; Xuan Chen; Kuberan Thangaraj; Anburaj Jeyaraj; Hoang Ha Nguyen; Ying Yu; Shunkai Hu; Li Lu; Xinghui Li. 2020. "Utilization of tea resources with the production of superparamagnetic biogenic iron oxide nanoparticles and an assessment of their antioxidant activities." Journal of Cleaner Production 278, no. : 123962.
Pruning is a routine management practice in tea cultivation. Although pruning is speculated to contribute to shoot growth and development in tea plants, it is imperative to understand the molecular mechanism involved. In order to investigate this, tea plants were pruned at different levels. Analysis of shoot growth indices revealed significant increase in shoots number and weight in shoots of pruned tea plant. Auxin assay showed higher concentrations of indole-3-acetic acid in pruned samples. Metabolomic analysis identified 80 differential metabolites in shoots of pruned plants, of which indole-3-acetonitrile and menaquinone were the common metabolites in all levels of pruning. The metabolites are involved in auxin biosynthesis, as shown by protein-protein interaction analysis. The metabolites enriched major metabolic pathways such as tryptophan metabolism, vitamin digestion and absorption, biosynthesis of ubiquinone and other terpenoid-quinone, and biosynthesis of amino acids. Genes involved in auxin signalling and menaquinone synthesis were up-regulated in pruned plants. This study reports, for the first time in nature, the synthesis of menaquinone in plants. This study concludes that pruning enhances shoot growth and development through the modulation of indole-3-acetic acid via synthesis of indole-3-acetonitrile and menaquinone in shoots, a combined effect of tryptophan metabolism and other metabolic pathways. This study contributes to knowledge in molecular mechanism of shoot growth and development.
Emmanuel Arkorful; Ying Yu; Changsong Chen; Li Lu; Shunkai Hu; Hanpu Yu; Qingping Ma; Kuberan Thangaraj; Rajiv Periakaruppan; Anburaj Jeyaraj; Xuan Chen; Xinghui Li. Untargeted metabolomic analysis using UPLC-MS/MS identifies metabolites involved in shoot growth and development in pruned tea plants (Camellia sinensis (L.) O. Kuntz). Scientia Horticulturae 2020, 264, 109164 .
AMA StyleEmmanuel Arkorful, Ying Yu, Changsong Chen, Li Lu, Shunkai Hu, Hanpu Yu, Qingping Ma, Kuberan Thangaraj, Rajiv Periakaruppan, Anburaj Jeyaraj, Xuan Chen, Xinghui Li. Untargeted metabolomic analysis using UPLC-MS/MS identifies metabolites involved in shoot growth and development in pruned tea plants (Camellia sinensis (L.) O. Kuntz). Scientia Horticulturae. 2020; 264 ():109164.
Chicago/Turabian StyleEmmanuel Arkorful; Ying Yu; Changsong Chen; Li Lu; Shunkai Hu; Hanpu Yu; Qingping Ma; Kuberan Thangaraj; Rajiv Periakaruppan; Anburaj Jeyaraj; Xuan Chen; Xinghui Li. 2020. "Untargeted metabolomic analysis using UPLC-MS/MS identifies metabolites involved in shoot growth and development in pruned tea plants (Camellia sinensis (L.) O. Kuntz)." Scientia Horticulturae 264, no. : 109164.
Melatonin is a biological hormone that plays crucial roles in stress tolerance. In this study, we investigated the effect of exogenous melatonin on abiotic stress in the tea plant. Under cold, salt and drought stress, increasing malondialdehyde levels and decreasing maximum photochemical efficiency of PSII were observed in tea leaves. Meanwhile, the levels of reactive oxygen species (ROS) increased significantly under abiotic stress. Interestingly, pretreatment with melatonin on leaves alleviated ROS burst, decreased malondialdehyde levels and maintain high photosynthetic efficiency. Moreover, 100 μM melatonin-pretreated tea plants showed high levels of glutathione and ascorbic acid and increased the activities of superoxide dismutase, peroxidase, catalase and ascorbate peroxidase under abiotic stress. Notably, melatonin treatments can positively up-regulate the genes (CsSOD, CsPOD, CsCAT and CsAPX) expression of antioxidant enzyme biosynthesis. Taken together, our results confirmed that melatonin protects tea plants against abiotic stress-induced damages through detoxifying ROS and regulating antioxidant systems.
Jiahao Li; Yiqing Yang; Kang Sun; Yi Chen; Xuan Chen; Xinghui Li. Exogenous Melatonin Enhances Cold, Salt and Drought Stress Tolerance by Improving Antioxidant Defense in Tea Plant (Camellia sinensis (L.) O. Kuntze). Molecules 2019, 24, 1826 .
AMA StyleJiahao Li, Yiqing Yang, Kang Sun, Yi Chen, Xuan Chen, Xinghui Li. Exogenous Melatonin Enhances Cold, Salt and Drought Stress Tolerance by Improving Antioxidant Defense in Tea Plant (Camellia sinensis (L.) O. Kuntze). Molecules. 2019; 24 (9):1826.
Chicago/Turabian StyleJiahao Li; Yiqing Yang; Kang Sun; Yi Chen; Xuan Chen; Xinghui Li. 2019. "Exogenous Melatonin Enhances Cold, Salt and Drought Stress Tolerance by Improving Antioxidant Defense in Tea Plant (Camellia sinensis (L.) O. Kuntze)." Molecules 24, no. 9: 1826.
We developed Cu-deficient, -sufficient and -super nutrition mice models by feeding them with diet containing 1.68, 11.72 or 51.69 mg of Cu/kg for 28 days, respectively. Then, the mice were treated to (−)-epigallocatechin-3-gallate (EGCG, 750 mg/kg BW) by oral in order to assess the acute toxicity of the drug. Following EGCG treatment, the survival rates were 12.5%, 50% and 100% in the Cu-deficient, -sufficient and Cu-super nutrition groups of mice, respectively. Cu level and ceruloplasmin activity in serum were significantly increased with the increase of dietary Cu. However, the Cu supplementation did not produce any obvious impact on serum superoxide dismutase activity. Furthermore, ceruloplasmin, in vitro, significantly promotes EGCG oxidation accompanied with increasing oxidation products and decreasing levels of reactive oxygen species. These results, therefore, suggest that Cu can relieve EGCG hepatotoxicity, possibly by up-regulating ceruloplasmin activity, which can be used to promote EGCG applications.
Najeeb Ahmed Kaleri; Kang Sun; Le Wang; Jin Li; Wenzheng Zhang; Xuan Chen; Xinghui Li. Dietary Copper Reduces the Hepatotoxicity of (−)-Epigallocatechin-3-Gallate in Mice. Molecules 2017, 23, 38 .
AMA StyleNajeeb Ahmed Kaleri, Kang Sun, Le Wang, Jin Li, Wenzheng Zhang, Xuan Chen, Xinghui Li. Dietary Copper Reduces the Hepatotoxicity of (−)-Epigallocatechin-3-Gallate in Mice. Molecules. 2017; 23 (1):38.
Chicago/Turabian StyleNajeeb Ahmed Kaleri; Kang Sun; Le Wang; Jin Li; Wenzheng Zhang; Xuan Chen; Xinghui Li. 2017. "Dietary Copper Reduces the Hepatotoxicity of (−)-Epigallocatechin-3-Gallate in Mice." Molecules 23, no. 1: 38.
When the homogenate of fresh tea tree leaves was fermented to produce black tea beverage, the Colletotrichum gloeosporioides (main pathogen or endophyte of Camellia sinensis) may be mixed into the fermentation liquor. However, it was unclear whether C. gloeosporioides-contaminated tea beverage would damage human health. Therefore, we investigated the changes of functional components and the influences on mice. C. gloeosporioides was added to the green tea infusion. After cultivation of 48 hours, tea polyphenols, caffeine and L-theanine decreased by 31.0 %, 26.2 % and 8.3 %, respectively. The contaminated tea infusion showed brown stain, and produced a group of toxic materials named phthalic acid esters. The animal study showed that green tea without contamination significantly decreased levels of alanine aminotransferase, triglycerides, free fatty acids, low density lipoprotein and increased insulin level compared with obese mice. On the contrary, contaminated tea lost the effects on these indicators. Furthermore, the urea nitrogen and serum creatinine levels significantly increased in the contaminated tea-drinking mice. Altogether, our results indicate that C. gloeosporioides contamination can reduce the amount of functional components of green tea. Therefore, it inhibits some health-care function of lipid-lowering. In addition, the toxic components in contaminated tea infusion might induce renal damage.
Jin Li; Kang Sun; Qingping Ma; Jin Chen; Le Wang; Dingjun Yang; Xuan Chen; Xinghui Li. Colletotrichum gloeosporioides- Contaminated Tea Infusion Blocks Lipids Reduction and Induces Kidney Damage in Mice. Frontiers in Microbiology 2017, 8, 2089 -2089.
AMA StyleJin Li, Kang Sun, Qingping Ma, Jin Chen, Le Wang, Dingjun Yang, Xuan Chen, Xinghui Li. Colletotrichum gloeosporioides- Contaminated Tea Infusion Blocks Lipids Reduction and Induces Kidney Damage in Mice. Frontiers in Microbiology. 2017; 8 ():2089-2089.
Chicago/Turabian StyleJin Li; Kang Sun; Qingping Ma; Jin Chen; Le Wang; Dingjun Yang; Xuan Chen; Xinghui Li. 2017. "Colletotrichum gloeosporioides- Contaminated Tea Infusion Blocks Lipids Reduction and Induces Kidney Damage in Mice." Frontiers in Microbiology 8, no. : 2089-2089.
CsHSP17.7, CsHSP18.1,andCsHSP21.8expressions are induced by heat and cold stresses, andCsHSPoverexpression confers tolerance to heat and cold stresses in transgenicPichia pastorisandArabidopsis thaliana. Small heat shock proteins (sHSPs) are crucial for protecting plants against biotic and abiotic stresses, especially heat stress. However, knowledge concerning the functions of Camellia sinensis sHSP in heat and cold stresses remains poorly understood. In this study, three C. sinensis sHSP genes (i.e., CsHSP17.7, CsHSP18.1, and CsHSP21.8) were isolated and characterized using suppression subtractive hybridization (SSH) technology. The CsHSPs expression levels in C. sinensis leaves were significantly up-regulated by heat and cold stresses. Phylogenetic analyses revealed that CsHSP17.7, CsHSP18.1, and CsHSP21.8 belong to sHSP Classes I, II, and IV, respectively. Heterologous expression of the three CsHSP genes in Pichia pastoris cells enhanced heat and cold stress tolerance. When exposed to heat and cold treatments, transgenic Arabidopsis thaliana plants overexpressing CsHSP17.7, CsHSP18.1, and CsHSP21.8 had lower malondialdehyde contents, ion leakage, higher proline contents, and transcript levels of stress-related genes (e.g., AtPOD, AtAPX1, AtP5CS2, and AtProT1) compared with the control line. In addition, improved seed germination vigor was also observed in the CsHSP-overexpressing seeds under heat stress. Taken together, our results suggest that the three identified CsHSP genes play key roles in heat and cold tolerance.
Mingle Wang; Zhongwei Zou; Qinghui Li; Huahong Xin; Xujun Zhu; Xuan Chen. Heterologous expression of three Camellia sinensis small heat shock protein genes confers temperature stress tolerance in yeast and Arabidopsis thaliana. Plant Cell Reports 2017, 36, 1125 -1135.
AMA StyleMingle Wang, Zhongwei Zou, Qinghui Li, Huahong Xin, Xujun Zhu, Xuan Chen. Heterologous expression of three Camellia sinensis small heat shock protein genes confers temperature stress tolerance in yeast and Arabidopsis thaliana. Plant Cell Reports. 2017; 36 (7):1125-1135.
Chicago/Turabian StyleMingle Wang; Zhongwei Zou; Qinghui Li; Huahong Xin; Xujun Zhu; Xuan Chen. 2017. "Heterologous expression of three Camellia sinensis small heat shock protein genes confers temperature stress tolerance in yeast and Arabidopsis thaliana." Plant Cell Reports 36, no. 7: 1125-1135.
Alzheimer's disease is a common neurodegenerative disorder in elderly. This study was aimed to systematically evaluate the association between tea intake and the risk of cognitive disorders by meta-analysis. PubMed, Embase and Wanfang databases were systematically searched and a total of 26 observational studies were included in this study. Odds ratios (ORs) and the corresponding 95% confidence intervals (CIs) were calculated and pooled by using fixed or random effects models according to the degree of heterogeneity. The overall pooled analysis indicated that tea intake could significantly reduce the risk of cognitive disorders (OR = 0.65, 95%CI = 0.58-0.73). Subgroup analyses were conducted based on study design, population, frequency of tea drinking and type of cognitive disorders. The results showed that tea drinking was significantly associated with the reduced incidence of cognitive disorders in all of subgroups based on study design and frequency of tea drinking. In particular, tea drinking was inversely associated with the risk of cognitive impairment (CoI), mild cognitive impairment (MCI), cognitive decline and ungrouped cognitive disorders. Moreover, for population subgroups, the significant association was only found in Chinese people. Our study suggests that daily tea drinking is associated with decreased risk of CoI, MCI and cognitive decline in the elderly. However, the association between tea intake and Alzheimer's disease remains elusive.
Qing-Ping Ma; Chen Huang; Qiao-Yun Cui; Ding-Jun Yang; Kang Sun; Xuan Chen; Xing-Hui Li. Meta-Analysis of the Association between Tea Intake and the Risk of Cognitive Disorders. PLOS ONE 2016, 11, e0165861 .
AMA StyleQing-Ping Ma, Chen Huang, Qiao-Yun Cui, Ding-Jun Yang, Kang Sun, Xuan Chen, Xing-Hui Li. Meta-Analysis of the Association between Tea Intake and the Risk of Cognitive Disorders. PLOS ONE. 2016; 11 (11):e0165861.
Chicago/Turabian StyleQing-Ping Ma; Chen Huang; Qiao-Yun Cui; Ding-Jun Yang; Kang Sun; Xuan Chen; Xing-Hui Li. 2016. "Meta-Analysis of the Association between Tea Intake and the Risk of Cognitive Disorders." PLOS ONE 11, no. 11: e0165861.
Flavonoids are the main flavor components and functional ingredients in tea, and the shikimic acid pathway is considered as one of the most important pathways in flavonoid biosynthesis, but little was known about the function of regulatory genes in the metabolism phenolic compounds in tea plant (Camellia sinensis), especially related genes in shikimic acid pathway. The dynamic changes of catechin (predominant flavonoid) contents were analyzed in this study, and four genes (CsPPT, CsDAHPS, CsSDH and CsCS) involving in shikimic acid pathway in C. sinensis albino cultivar ‘Baicha 1’ were cloned and characterized. The full-length cDNA sequences of these genes were obtained using reverse transcription-PCR and rapid amplification of cDNA ends. At the albinistic stage, the amounts of all catechins decreased to the lowest levels, when epigallocatechin gallate was the highest, whereas gallocatechin-3-O-gallate the lowest. Gene expression patterns analyzed by qRT-PCR showed that CsPPT and CsDAHPS were highly expressed in flowers and buds, while CsSDH and CsCS showed high expression levels in buds and leaves. It was also found that the transcript abundance of shikimic acid biosynthetic genes followed a tightly regulated biphasic pattern, and was affected by albinism. The transcript levels of CsPPT and CsDAHPS were decreased at albinistic stage followed elevated expression, whereas CsSDH and CsCS were increased only at re-greening stage. Taken together, these findings suggested that these four genes in C. sinensis may play different roles in shikimic acid biosynthesis and these genes may have divergent functions.
Xu-Jun Zhu; Zhen Zhao; Hua-Hong Xin; Ming-Le Wang; Wei-Dong Wang; Xuan Chen; Xing-Hui Li. Isolation and dynamic expression of four genes involving in shikimic acid pathway in Camellia sinensis ‘Baicha 1’ during periodic albinism. Molecular Biology Reports 2016, 43, 1119 -1127.
AMA StyleXu-Jun Zhu, Zhen Zhao, Hua-Hong Xin, Ming-Le Wang, Wei-Dong Wang, Xuan Chen, Xing-Hui Li. Isolation and dynamic expression of four genes involving in shikimic acid pathway in Camellia sinensis ‘Baicha 1’ during periodic albinism. Molecular Biology Reports. 2016; 43 (10):1119-1127.
Chicago/Turabian StyleXu-Jun Zhu; Zhen Zhao; Hua-Hong Xin; Ming-Le Wang; Wei-Dong Wang; Xuan Chen; Xing-Hui Li. 2016. "Isolation and dynamic expression of four genes involving in shikimic acid pathway in Camellia sinensis ‘Baicha 1’ during periodic albinism." Molecular Biology Reports 43, no. 10: 1119-1127.
Xuan Chen; Lei Yang; Longjiao Hu; Kangcai Wang. [Dynamic accumulation of dry matter and active element in Pinellia ternata of Taizhou]. China Journal of Chinese Materia Medica 2012, 37, 1 .
AMA StyleXuan Chen, Lei Yang, Longjiao Hu, Kangcai Wang. [Dynamic accumulation of dry matter and active element in Pinellia ternata of Taizhou]. China Journal of Chinese Materia Medica. 2012; 37 (7):1.
Chicago/Turabian StyleXuan Chen; Lei Yang; Longjiao Hu; Kangcai Wang. 2012. "[Dynamic accumulation of dry matter and active element in Pinellia ternata of Taizhou]." China Journal of Chinese Materia Medica 37, no. 7: 1.
Xuan Chen; Jiale Zhou; Xiaoqing Tang; Kangcai Wang. [Effect of water stress on content of four organic acids in different cultivated populations of Isatis indigotica]. China Journal of Chinese Materia Medica 2009, 34, 1 .
AMA StyleXuan Chen, Jiale Zhou, Xiaoqing Tang, Kangcai Wang. [Effect of water stress on content of four organic acids in different cultivated populations of Isatis indigotica]. China Journal of Chinese Materia Medica. 2009; 34 (24):1.
Chicago/Turabian StyleXuan Chen; Jiale Zhou; Xiaoqing Tang; Kangcai Wang. 2009. "[Effect of water stress on content of four organic acids in different cultivated populations of Isatis indigotica]." China Journal of Chinese Materia Medica 34, no. 24: 1.
Xuan Chen; Kang-Cai Wang; Mei-Li Wu; Yue-Yu Hang; Yi-Feng Zhou. [Study on balanced application of NPK fertilizer on growth of Paeonia lactiflora with orthogonal design]. Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials 2008, 31, 1 .
AMA StyleXuan Chen, Kang-Cai Wang, Mei-Li Wu, Yue-Yu Hang, Yi-Feng Zhou. [Study on balanced application of NPK fertilizer on growth of Paeonia lactiflora with orthogonal design]. Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials. 2008; 31 (6):1.
Chicago/Turabian StyleXuan Chen; Kang-Cai Wang; Mei-Li Wu; Yue-Yu Hang; Yi-Feng Zhou. 2008. "[Study on balanced application of NPK fertilizer on growth of Paeonia lactiflora with orthogonal design]." Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials 31, no. 6: 1.