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Mathieu Mahillon
Earth and Life Institute, Applied Microbiology-Phytopathology, UCLouvain, Louvain-la-Neuve, Belgium

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Short communication
Published: 11 March 2021 in Virus Research
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This study describes a new mycovirus infecting a strain from the Fusarium incarnatum-equiseti species complex. Based on phylogenetic and genomic analyses, this virus belongs to the recently proposed genus “Zetapartitivirus” in the family Partitiviridae. The name “Fusarium equiseti partitivirus 1″ (FePV1) is therefore suggested for this novel viral species. Similar to other partitiviruses, FePV1 genome is composed by two dsRNA segments that exhibit each one large ORF encoding for an RdRp and a CP, respectively. A smaller dsRNA was also detected in infected mycelium and could be a satellite RNA of FePV1. In addition to characterized zetapartitiviruses, other FePV1-related sequences were retrieved from online databases and their significance is discussed. Following conidial isolation, an FePV1-free isogenic line of the fungal host was obtained. In comparison with the original infected strain, this line showed higher growth, biomass production and pathogenicity on tomato, advocating that FePV1 induces hypovirulence on its host.

ACS Style

Mathieu Mahillon; Alain Decroës; Simon Caulier; Assiata Tiendrebeogo; Anne Legrève; Claude Bragard. Genomic and biological characterization of a novel partitivirus infecting Fusarium equiseti. Virus Research 2021, 297, 198386 .

AMA Style

Mathieu Mahillon, Alain Decroës, Simon Caulier, Assiata Tiendrebeogo, Anne Legrève, Claude Bragard. Genomic and biological characterization of a novel partitivirus infecting Fusarium equiseti. Virus Research. 2021; 297 ():198386.

Chicago/Turabian Style

Mathieu Mahillon; Alain Decroës; Simon Caulier; Assiata Tiendrebeogo; Anne Legrève; Claude Bragard. 2021. "Genomic and biological characterization of a novel partitivirus infecting Fusarium equiseti." Virus Research 297, no. : 198386.

Journal article
Published: 01 February 2021 in Journal of General Virology
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Beet soil-borne virus (BSBV) is a sugar beet pomovirus frequently associated with Beet necrotic yellow veins virus, the causal agent of the rhizomania disease. BSBV has been detected in most of the major beet-growing regions worldwide, yet its impact on this crop remains unclear. With the aim to understand the life cycle of this virus and clarify its putative pathogenicity, agroinfectious clones have been engineered for each segment of its tripartite genome. The biological properties of these clones were then studied on different plant species. Local infection was obtained on agroinfiltrated leaves of Beta macrocarpa. On leaves of Nicotiana benthamiana, similar results were obtained, but only when heterologous viral suppressors of RNA silencing were co-expressed or in a transgenic line down regulated for both dicer-like protein 2 and 4. On sugar beet, local infection following agroinoculation was obtained on cotyledons, but not on other tested plant parts. Nevertheless, leaf symptoms were observed on this host via sap inoculation. Likewise, roots were efficiently mechanically infected, highlighting low frequency of root necrosis and constriction, and enabling the demonstration of transmission by the vector Polymyxa betae. Altogether, the entire viral cycle was reproduced, validating the constructed agroclones as efficient inoculation tools, paving the way for further studies on BSBV and its related pathosystem.

ACS Style

Mathieu Mahillon; Alain Decroës; Chloé Peduzzi; Gustavo Romay; Anne Legrève; Claude Bragard. RNA silencing machinery contributes to inability of BSBV to establish infection in Nicotiana benthamiana: evidence from characterization of agroinfectious clones of Beet soil-borne virus. Journal of General Virology 2021, 102, jgv001530 .

AMA Style

Mathieu Mahillon, Alain Decroës, Chloé Peduzzi, Gustavo Romay, Anne Legrève, Claude Bragard. RNA silencing machinery contributes to inability of BSBV to establish infection in Nicotiana benthamiana: evidence from characterization of agroinfectious clones of Beet soil-borne virus. Journal of General Virology. 2021; 102 (2):jgv001530.

Chicago/Turabian Style

Mathieu Mahillon; Alain Decroës; Chloé Peduzzi; Gustavo Romay; Anne Legrève; Claude Bragard. 2021. "RNA silencing machinery contributes to inability of BSBV to establish infection in Nicotiana benthamiana: evidence from characterization of agroinfectious clones of Beet soil-borne virus." Journal of General Virology 102, no. 2: jgv001530.

Communication
Published: 09 May 2020 in Viruses
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A new mycovirus was found in the Fusarium culmorum strain A104-1 originally sampled on wheat in Belgium. This novel virus, for which the name Fusarium culmorum virus 1 (FcV1) is suggested, is phylogenetically related to members of the previously proposed family ‘’Unirnaviridae’’. FcV1 has a monopartite dsRNA genome of 2898 bp that harbors two large non-overlapping ORFs. A typical -1 slippery motif is found at the end of ORF1, advocating that ORF2 is translated by programmed ribosomal frameshifting. While ORF2 exhibits a conserved replicase domain, ORF1 encodes for an undetermined protein. Interestingly, a hypothetically transcribed gene similar to unirnaviruses ORF1 was found in the genome of Lipomyces starkeyi, presumably resulting from a viral endogenization in this yeast. Conidial isolation and chemical treatment were unsuccessful to obtain a virus-free isogenic line of the fungal host, highlighting a high retention rate for FcV1 but hindering its biological characterization. In parallel, attempt to horizontally transfer FcV1 to another strain of F. culmorum by dual culture failed. Eventually, a screening of other strains of the same fungal species suggests the presence of FcV1 in two other strains from Europe.

ACS Style

Mathieu Mahillon; Gustavo Romay; Charlotte Liénard; Anne Legrève; Claude Bragard. Description of a Novel Mycovirus in the Phytopathogen Fusarium culmorum and a Related EVE in the Yeast Lipomyces starkeyi. Viruses 2020, 12, 523 .

AMA Style

Mathieu Mahillon, Gustavo Romay, Charlotte Liénard, Anne Legrève, Claude Bragard. Description of a Novel Mycovirus in the Phytopathogen Fusarium culmorum and a Related EVE in the Yeast Lipomyces starkeyi. Viruses. 2020; 12 (5):523.

Chicago/Turabian Style

Mathieu Mahillon; Gustavo Romay; Charlotte Liénard; Anne Legrève; Claude Bragard. 2020. "Description of a Novel Mycovirus in the Phytopathogen Fusarium culmorum and a Related EVE in the Yeast Lipomyces starkeyi." Viruses 12, no. 5: 523.

Annotated sequence record
Published: 04 June 2019 in Archives of Virology
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By screening a collection of Fusarium spp. for the presence of dsRNA, the Fusarium redolens strain A63-1 was found harboring a pattern of multiple dsRNA bands when analyzed by agarose gel electrophoresis. Using NextSeq Illumina sequencing, the full sequences of eight dsRNA molecules were determined, compared to databases, and gathered into a new viral genome. This novel virus shares similarities with mycoviruses that were recently grouped in the proposed family “Polymycoviridae”. Hence, the name “Fusarium redolens polymycovirus 1” is proposed for this virus. Each viral dsRNA contains only one ORF, except dsRNA 7, which has an additional one. Based on amino acid sequence similarities, the functions of the proteins encoded by dsRNA 1–4 can be hypothesized. On the other hand, the putative proteins encoded by dsRNA 5–8 exhibit no relevant homology to known proteins. In this report, the full genome sequence of this new virus is presented along with a primary bioinformatics analysis.

ACS Style

Mathieu Mahillon; Alain Decroës; Charlotte Liénard; Claude Bragard; Anne Legrève. Full genome sequence of a new polymycovirus infecting Fusarium redolens. Archives of Virology 2019, 164, 2215 -2219.

AMA Style

Mathieu Mahillon, Alain Decroës, Charlotte Liénard, Claude Bragard, Anne Legrève. Full genome sequence of a new polymycovirus infecting Fusarium redolens. Archives of Virology. 2019; 164 (8):2215-2219.

Chicago/Turabian Style

Mathieu Mahillon; Alain Decroës; Charlotte Liénard; Claude Bragard; Anne Legrève. 2019. "Full genome sequence of a new polymycovirus infecting Fusarium redolens." Archives of Virology 164, no. 8: 2215-2219.

Communication
Published: 04 April 2019 in Viruses
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Begomoviruses are one of the major groups of plant viruses with an important economic impact on crop production in tropical and subtropical regions. The global spread of its polyphagous vector, the whitefly Bemisia tabaci, has contributed to the emergence and diversification of species within this genus. In this study, we found a putative novel begomovirus infecting tomato plants in Venezuela without a cognate DNA-B component. This begomovirus was genetically characterized and compared with related species. Furthermore, its infectivity was demonstrated by agroinoculation of infectious clones in tomato (Solanum lycopersicum) and Nicotiana benthamiana plants. The name Tomato twisted leaf virus (ToTLV) is proposed. ToTLV showed the typical genome organization of the DNA-A component of New World bipartite begomoviruses. However, the single DNA component of ToTLV was able to develop systemic infection in tomato and N. benthamiana plants, suggesting a monopartite nature of its genome. Interestingly, an additional open reading frame ORF was observed in ToTLV encompassing the intergenic region and the coat protein gene, which is not present in other closely related begomoviruses. A putative transcript from this region was amplified by strand-specific reverse transcription-PCR. Along with recent studies, our results showed that the diversity of monopartite begomoviruses from the New World is greater than previously thought.

ACS Style

Gustavo Romay; Francis Geraud-Pouey; Dorys T. Chirinos; Mathieu Mahillon; Annika Gillis; Jacques Mahillon; Claude Bragard. Tomato Twisted Leaf Virus: A Novel Indigenous New World Monopartite Begomovirus Infecting Tomato in Venezuela. Viruses 2019, 11, 327 .

AMA Style

Gustavo Romay, Francis Geraud-Pouey, Dorys T. Chirinos, Mathieu Mahillon, Annika Gillis, Jacques Mahillon, Claude Bragard. Tomato Twisted Leaf Virus: A Novel Indigenous New World Monopartite Begomovirus Infecting Tomato in Venezuela. Viruses. 2019; 11 (4):327.

Chicago/Turabian Style

Gustavo Romay; Francis Geraud-Pouey; Dorys T. Chirinos; Mathieu Mahillon; Annika Gillis; Jacques Mahillon; Claude Bragard. 2019. "Tomato Twisted Leaf Virus: A Novel Indigenous New World Monopartite Begomovirus Infecting Tomato in Venezuela." Viruses 11, no. 4: 327.