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Alexandre Campos
CIIMAR, Interdisciplinary Centre of Marine and Environmental Research, Terminal de Cruzeiros do Porto de Leixões, Av. General Norton de Matos, s/n, 4450-208 Porto, Portugal

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Journal article
Published: 16 August 2021 in Microorganisms
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Frequent toxic cyanoblooms in eutrophic freshwaters produce various cyanotoxins such as the monocyclic heptapeptides microcystins (MCs), known as deleterious compounds to plant growth and human health. Recently, MCs are a recurrent worldwide sanitary problem in irrigation waters and farmland soils due to their transfer and accumulation in the edible tissues of vegetable produce. In such cases, studies about the persistence and removal of MCs in soil are scarce and not fully investigated. In this study, we carried out a greenhouse trial on two crop species: faba bean (Vicia faba var. Alfia 321) and common wheat (Triticum aestivum var. Achtar) that were grown in sterile (microorganism-free soil) and non-sterile (microorganism-rich soil) soils and subjected to MC-induced stress at 100 µg equivalent MC-LR L−1. The experimentation aimed to assess the prominent role of native rhizospheric microbiota in mitigating the phytotoxic impact of MCs on plant growth and reducing their accumulation in both soils and plant tissues. Moreover, we attempted to evaluate the health risk related to the consumption of MC-polluted plants for humans and cattle by determining the estimated daily intake (EDI) and health risk quotient (RQ) of MCs in these plants. Biodegradation was liable to be the main removal pathway of the toxin in the soil; and therefore, bulk soil (unplanted soil), as well as rhizospheric soil (planted soil), were used in this experiment to evaluate the accumulation of MCs in the presence and absence of microorganisms (sterile and non-sterile soils). The data obtained in this study showed that MCs had no significant effects on growth indicators of faba bean and common wheat plants in non-sterile soil as compared to the control group. In contrast, plants grown in sterile soil showed a significant decrease in growth parameters as compared to the control. These results suggest that MCs were highly bioavailable to the plants, resulting in severe growth impairments in the absence of native rhizospheric microbiota. Likewise, MCs were more accumulated in sterile soil and more bioconcentrated in root and shoot tissues of plants grown within when compared to non-sterile soil. Thereby, the EDI of MCs in plants grown in sterile soil was more beyond the tolerable daily intake recommended for both humans and cattle. The risk level was more pronounced in plants from the sterile soil than those from the non-sterile one. These findings suggest that microbial activity, eventually MC-biodegradation, is a crucial bioremediation tool to remove and prevent MCs from entering the agricultural food chain.

ACS Style

El Mahdi Redouane; Richard Mugani; Majida Lahrouni; José Carlos Martins; Soukaina El Amrani Zerrifi; Khalid Oufdou; Alexandre Campos; Vitor Vasconcelos; Brahim Oudra. Role of Rhizospheric Microbiota as a Bioremediation Tool for the Protection of Soil-Plant Systems from Microcystins Phytotoxicity and Mitigating Toxin-Related Health Risk. Microorganisms 2021, 9, 1747 .

AMA Style

El Mahdi Redouane, Richard Mugani, Majida Lahrouni, José Carlos Martins, Soukaina El Amrani Zerrifi, Khalid Oufdou, Alexandre Campos, Vitor Vasconcelos, Brahim Oudra. Role of Rhizospheric Microbiota as a Bioremediation Tool for the Protection of Soil-Plant Systems from Microcystins Phytotoxicity and Mitigating Toxin-Related Health Risk. Microorganisms. 2021; 9 (8):1747.

Chicago/Turabian Style

El Mahdi Redouane; Richard Mugani; Majida Lahrouni; José Carlos Martins; Soukaina El Amrani Zerrifi; Khalid Oufdou; Alexandre Campos; Vitor Vasconcelos; Brahim Oudra. 2021. "Role of Rhizospheric Microbiota as a Bioremediation Tool for the Protection of Soil-Plant Systems from Microcystins Phytotoxicity and Mitigating Toxin-Related Health Risk." Microorganisms 9, no. 8: 1747.

Journal article
Published: 18 May 2021 in Water
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Cyanobacteria blooms occur frequently in freshwaters around the world. Some can produce and release toxic compounds called cyanotoxins, which represent a danger to both the environment and human health. Microcystin-LR (MC-LR) is the most toxic variant reported all over the world. Conventional water treatment methods are expensive and require specialized personnel and equipment. Recently, a multi-soil-layering (MSL) system, a natural and low-cost technology, has been introduced as an attractive cost-effective, and environmentally friendly technology that is likely to be an alternative to conventional wastewater treatment methods. This study aims to evaluate, for the first time, the efficiency of MSL eco-technology to remove MC-LR on a laboratory scale using local materials. To this end, an MSL pilot plant was designed to treat distilled water contaminated with MC-LR. The pilot was composed of an alternation of permeable layers (pozzolan) and soil mixture layers (local sandy soil, sawdust, charcoal, and metallic iron on a dry weight ratio of 70, 10, 10, and 10%, respectively) arranged in a brick-layer-like pattern. MSL pilot was continuously fed with synthetic water containing distilled water contaminated with increasing concentrations of MC-LR (0.18–10 µg/L) at a hydraulic loading rate (HLR) of 200 L m−2 day−1. The early results showed MC-LR removal of above 99%. Based on these preliminary results, the multi-soil-layering eco-technology could be considered as a promising solution to treat water contaminated by MC-LR in order to produce quality water for irrigation or recreational activities.

ACS Style

Roseline Aba; Richard Mugani; Abdessamad Hejjaj; Nelly Brugerolle de Fraissinette; Brahim Oudra; Naaila Ouazzani; Alexandre Campos; Vitor Vasconcelos; Pedro Carvalho; Laila Mandi. First Report on Cyanotoxin (MC-LR) Removal from Surface Water by Multi-Soil-Layering (MSL) Eco-Technology: Preliminary Results. Water 2021, 13, 1403 .

AMA Style

Roseline Aba, Richard Mugani, Abdessamad Hejjaj, Nelly Brugerolle de Fraissinette, Brahim Oudra, Naaila Ouazzani, Alexandre Campos, Vitor Vasconcelos, Pedro Carvalho, Laila Mandi. First Report on Cyanotoxin (MC-LR) Removal from Surface Water by Multi-Soil-Layering (MSL) Eco-Technology: Preliminary Results. Water. 2021; 13 (10):1403.

Chicago/Turabian Style

Roseline Aba; Richard Mugani; Abdessamad Hejjaj; Nelly Brugerolle de Fraissinette; Brahim Oudra; Naaila Ouazzani; Alexandre Campos; Vitor Vasconcelos; Pedro Carvalho; Laila Mandi. 2021. "First Report on Cyanotoxin (MC-LR) Removal from Surface Water by Multi-Soil-Layering (MSL) Eco-Technology: Preliminary Results." Water 13, no. 10: 1403.

Review
Published: 11 May 2021 in Applied Sciences
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Cyanobacteria have long been associated with harmful effects on humans, animals and aquatic biota. Cyanotoxins are their most toxic metabolite. This review summarizes the current research, impacts and future needs in cyanobacterial studies undertaken in Portugal, the southernmost country of Europe, and with a recent multiplication of cyanotoxicity due to climate change events. Microcystins are still the most prevalent, studied and the only regulated cyanotoxins in Portuguese freshwater systems much like most European countries. With the development of some tools, particularly in molecular studies, the recent discovery of cylindrospermopsins, anatoxins and saxitoxins, both genes and toxins, in North and Center ecosystems of our country highlight current impacts that overall communities are facing with increased risks of exposure and uptake to cyanotoxins. Research needs encompass the expansion of studies at all aspects due to the uprising of these cyanotoxins and reinforces the urgent need of increasing the frequency of surveillance to achieve tangible effects of cyanotoxins in Portugal to ultimately implement regulations on cylindrospermopsins, anatoxins and saxitoxins worldwide.

ACS Style

Cristiana Moreira; Alexandre Campos; José Martins; Vitor Vasconcelos; Agostinho Antunes. Review on Cyanobacterial Studies in Portugal: Current Impacts and Research Needs. Applied Sciences 2021, 11, 4355 .

AMA Style

Cristiana Moreira, Alexandre Campos, José Martins, Vitor Vasconcelos, Agostinho Antunes. Review on Cyanobacterial Studies in Portugal: Current Impacts and Research Needs. Applied Sciences. 2021; 11 (10):4355.

Chicago/Turabian Style

Cristiana Moreira; Alexandre Campos; José Martins; Vitor Vasconcelos; Agostinho Antunes. 2021. "Review on Cyanobacterial Studies in Portugal: Current Impacts and Research Needs." Applied Sciences 11, no. 10: 4355.

Review
Published: 28 March 2021 in Plants
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Cyanobacteria are a group of photosynthetic prokaryotes that pose a great concern in the aquatic environments related to contamination and poisoning of wild life and humans. Some species of cyanobacteria produce potent toxins such as microcystins (MCs), which are extremely aggressive to several organisms, including animals and humans. In order to protect human health and prevent human exposure to this type of organisms and toxins, regulatory limits for MCs in drinking water have been established in most countries. In this regard, the World Health Organization (WHO) proposed 1 µg MCs/L as the highest acceptable concentration in drinking water. However, regulatory limits were not defined in waters used in other applications/activities, constituting a potential threat to the environment and to human health. Indeed, water contaminated with MCs or other cyanotoxins is recurrently used in agriculture and for crop and food production. Several deleterious effects of MCs including a decrease in growth, tissue necrosis, inhibition of photosynthesis and metabolic changes have been reported in plants leading to the impairment of crop productivity and economic loss. Studies have also revealed significant accumulation of MCs in edible tissues and plant organs, which raise concerns related to food safety. This work aims to systematize and analyze the information generated by previous scientific studies, namely on the phytotoxicity and the impact of MCs especially on growth, photosynthesis and productivity of agricultural plants. Morphological and physiological parameters of agronomic interest are overviewed in detail in this work, with the aim to evaluate the putative impact of MCs under field conditions. Finally, concentration-dependent effects are highlighted, as these can assist in future guidelines for irrigation waters and establish regulatory limits for MCs.

ACS Style

Alexandre Campos; El Redouane; Marisa Freitas; Samuel Amaral; Tomé Azevedo; Leticia Loss; Csaba Máthé; Zakaria Mohamed; Brahim Oudra; Vitor Vasconcelos. Impacts of Microcystins on Morphological and Physiological Parameters of Agricultural Plants: A Review. Plants 2021, 10, 639 .

AMA Style

Alexandre Campos, El Redouane, Marisa Freitas, Samuel Amaral, Tomé Azevedo, Leticia Loss, Csaba Máthé, Zakaria Mohamed, Brahim Oudra, Vitor Vasconcelos. Impacts of Microcystins on Morphological and Physiological Parameters of Agricultural Plants: A Review. Plants. 2021; 10 (4):639.

Chicago/Turabian Style

Alexandre Campos; El Redouane; Marisa Freitas; Samuel Amaral; Tomé Azevedo; Leticia Loss; Csaba Máthé; Zakaria Mohamed; Brahim Oudra; Vitor Vasconcelos. 2021. "Impacts of Microcystins on Morphological and Physiological Parameters of Agricultural Plants: A Review." Plants 10, no. 4: 639.

Journal article
Published: 25 March 2021 in International Journal of Molecular Sciences
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We focus on the stalked goose barnacle L. anatifera adhesive system, an opportunistic less selective species for the substrate, found attached to a variety of floating objects at seas. Adhesion is an adaptative character in barnacles, ensuring adequate positioning in the habitat for feeding and reproduction. The protein composition of the cement multicomplex and adhesive gland was quantitatively studied using shotgun proteomic analysis. Overall, 11,795 peptide sequences were identified in the gland and 2206 in the cement, clustered in 1689 and 217 proteinGroups, respectively. Cement specific adhesive proteins (CPs), proteases, protease inhibitors, cuticular and structural proteins, chemical cues, and many unannotated proteins were found, among others. In the cement, CPs were the most abundant (80.5%), being the bulk proteins CP100k and -52k the most expressed of all, and CP43k-like the most expressed interfacial protein. Unannotated proteins comprised 4.7% of the cement proteome, ranking several of them among the most highly expressed. Eight of these proteins showed similar physicochemical properties and amino acid composition to known CPs and classified through Principal Components Analysis (PCA) as new CPs. The importance of PCA on the identification of unannotated non-conserved adhesive proteins, whose selective pressure is on their relative amino acid abundance, was demonstrated.

ACS Style

Dany Domínguez-Pérez; Daniela Almeida; Josef Wissing; André Machado; Lothar Jänsch; Agostinho Antunes; Luís Castro; Vitor Vasconcelos; Alexandre Campos; Isabel Cunha. Proteogenomic Characterization of the Cement and Adhesive Gland of the Pelagic Gooseneck Barnacle Lepas anatifera. International Journal of Molecular Sciences 2021, 22, 3370 .

AMA Style

Dany Domínguez-Pérez, Daniela Almeida, Josef Wissing, André Machado, Lothar Jänsch, Agostinho Antunes, Luís Castro, Vitor Vasconcelos, Alexandre Campos, Isabel Cunha. Proteogenomic Characterization of the Cement and Adhesive Gland of the Pelagic Gooseneck Barnacle Lepas anatifera. International Journal of Molecular Sciences. 2021; 22 (7):3370.

Chicago/Turabian Style

Dany Domínguez-Pérez; Daniela Almeida; Josef Wissing; André Machado; Lothar Jänsch; Agostinho Antunes; Luís Castro; Vitor Vasconcelos; Alexandre Campos; Isabel Cunha. 2021. "Proteogenomic Characterization of the Cement and Adhesive Gland of the Pelagic Gooseneck Barnacle Lepas anatifera." International Journal of Molecular Sciences 22, no. 7: 3370.

Journal article
Published: 05 February 2021 in Toxins
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Microcystins (MCs) produced in eutrophic waters may decrease crop yield, enter food chains and threaten human and animal health. The main objective of this research was to highlight the role of rhizospheric soil microbiota to protect faba bean plants from MCs toxicity after chronic exposure. Faba bean seedlings were grown in pots containing agricultural soil, during 1 month under natural environmental conditions of Marrakech city in Morocco (March–April 2018) and exposed to cyanobacterial extracts containing up to 2.5 mg·L−1 of total MCs. Three independent exposure experiments were performed (a) agricultural soil was maintained intact “exposure experiment 1”; (b) agricultural soil was sterilized “exposure experiment 2”; (c) agricultural soil was sterilized and inoculated with the rhizobia strain Rhizobium leguminosarum RhOF34 “exposure experiment 3”. Overall, data showed evidence of an increased sensitivity of faba bean plants, grown in sterilized soil, to MCs in comparison to those grown in intact and inoculated soils. The study revealed the growth inhibition of plant shoots in both exposure experiments 2 and 3 when treated with 2.5 mg·L−1 of MCs. The results also showed that the estimated daily intake (EDI) of MCs, in sterilized soil, exceeded 2.18 and 1.16 times the reference concentrations (0.04 and 0.45 µg of microcysin-leucine arginine (MC-LR). Kg−1 DW) established for humans and cattle respectively, which raises concerns about human food chain contamination.

ACS Style

El Redouane; Majida Lahrouni; José Martins; Soukaina El Amrani Zerrifi; Loubna Benidire; Mountassir Douma; Faissal Aziz; Khalid Oufdou; Laila Mandi; Alexandre Campos; Vitor Vasconcelos; Brahim Oudra. Protective Role of Native Rhizospheric Soil Microbiota Against the Exposure to Microcystins Introduced into Soil-Plant System via Contaminated Irrigation Water and Health Risk Assessment. Toxins 2021, 13, 118 .

AMA Style

El Redouane, Majida Lahrouni, José Martins, Soukaina El Amrani Zerrifi, Loubna Benidire, Mountassir Douma, Faissal Aziz, Khalid Oufdou, Laila Mandi, Alexandre Campos, Vitor Vasconcelos, Brahim Oudra. Protective Role of Native Rhizospheric Soil Microbiota Against the Exposure to Microcystins Introduced into Soil-Plant System via Contaminated Irrigation Water and Health Risk Assessment. Toxins. 2021; 13 (2):118.

Chicago/Turabian Style

El Redouane; Majida Lahrouni; José Martins; Soukaina El Amrani Zerrifi; Loubna Benidire; Mountassir Douma; Faissal Aziz; Khalid Oufdou; Laila Mandi; Alexandre Campos; Vitor Vasconcelos; Brahim Oudra. 2021. "Protective Role of Native Rhizospheric Soil Microbiota Against the Exposure to Microcystins Introduced into Soil-Plant System via Contaminated Irrigation Water and Health Risk Assessment." Toxins 13, no. 2: 118.

Original research article
Published: 16 December 2020 in Frontiers in Marine Science
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The molecular pathways behind the toxicity of diarrheic shellfish toxins (DSTs) in bivalves have been scarcely studied. Thus, a shotgun proteomics approach was applied in this work to understand bivalves’ molecular responses to the dinoflagellate Prorocentrum lima (1.0 × 106 cells/L). Protein expression along with toxins levels were analyzed in the gills and digestive gland of the mussel Mytilus galloprovincialis during and after exposure to this toxic strain. Results revealed an accumulation of OA and DTX1 only in the digestive gland with maximum amounts attained at the end of uptake phase (day 5; 2819.2 ± 522.2 μg OA/kg and 1107.1 ± 267.9 μg DTX1/kg). At the end of the depuration phase (day 20), 16% and 47% of total OA and DTX1 concentrations remained in the digestive gland tissues, respectively. The shotgun proteomic analyses yielded 3051 proteins in both organs. A total of 56 and 54 differentially expressed proteins (DEPs) were revealed in the digestive gland and gills, respectively. Both organs presented the same response dynamics along the experiment, although with tissue-specific features. The early response (3 days uptake) was characterized by a high number of DEPs, being more marked in gills, in relation to the latter time points (5 days uptake and depuration). Functional enrichment analysis revealed the up-regulation of carboxylic (GO:0046943) and organic acid transmembrane transporter activity (GO:0005342) pathways after 3 days uptake for digestive gland. Matching to these pathways are a group of proteins related to transmembrane transport and response to toxic substances and xenobiotics, namely P-glycoprotein (ABCB11), Sodium-dependent proline transporter (SLC6A7), and Sideroflexin-1 (SFXN1). According to Clusters of Orthologous Groups (GOs) categories, most of the DEPs found for digestive gland in all time-points were related with “cellular processes and signaling” and involving signal transduction mechanisms, cytoskeleton and post-translational modification, protein turnover, chaperone functions. In gills, the early uptake phase was marked by a balance between DEPs related with “cellular processes and signaling” and “metabolism.” Depuration is clearly marked by processes related with “metabolism,” mainly involving secondary metabolites biosynthesis, transport, and catabolism. Proteomic data are available via ProteomeXchange with identifier PXD022293.

ACS Style

José Carlos Martins; Dany Domínguez-Pérez; Catarina Azevedo; Ana Catarina Braga; Pedro Reis Costa; Hugo Osório; Vitor Vasconcelos; Alexandre Campos. Molecular Responses of Mussel Mytilus galloprovincialis Associated to Accumulation and Depuration of Marine Biotoxins Okadaic Acid and Dinophysistoxin-1 Revealed by Shotgun Proteomics. Frontiers in Marine Science 2020, 7, 1 .

AMA Style

José Carlos Martins, Dany Domínguez-Pérez, Catarina Azevedo, Ana Catarina Braga, Pedro Reis Costa, Hugo Osório, Vitor Vasconcelos, Alexandre Campos. Molecular Responses of Mussel Mytilus galloprovincialis Associated to Accumulation and Depuration of Marine Biotoxins Okadaic Acid and Dinophysistoxin-1 Revealed by Shotgun Proteomics. Frontiers in Marine Science. 2020; 7 ():1.

Chicago/Turabian Style

José Carlos Martins; Dany Domínguez-Pérez; Catarina Azevedo; Ana Catarina Braga; Pedro Reis Costa; Hugo Osório; Vitor Vasconcelos; Alexandre Campos. 2020. "Molecular Responses of Mussel Mytilus galloprovincialis Associated to Accumulation and Depuration of Marine Biotoxins Okadaic Acid and Dinophysistoxin-1 Revealed by Shotgun Proteomics." Frontiers in Marine Science 7, no. : 1.

Data descriptor
Published: 27 November 2020 in Data
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Here we provide all datasets and details applied in the construction of a composite protein database required for the proteogenomic analyses of the article “Putative Antimicrobial Peptides of the Posterior Salivary Glands from the Cephalopod Octopus vulgaris Revealed by Exploring a Composite Protein Database”. All data, subdivided into six datasets, are deposited at the Mendeley Data repository as follows. Dataset_1 provides our composite database “All_Databases_5950827_sequences.fasta” derived from six smaller databases composed of (i) protein sequences retrieved from public databases related to cephalopods’ salivary glands, (ii) proteins identified with Proteome Discoverer software using our original data obtained by shotgun proteomic analyses of posterior salivary glands (PSGs) from three Octopus vulgaris specimens (provided as Dataset_2) and (iii) a non-redundant antimicrobial peptide (AMP) database. Dataset_3 includes the transcripts obtained by de novo assembly of 16 transcriptomes from cephalopods’ PSGs using CLC Genomics Workbench. Dataset_4 provides the proteins predicted by the TransDecoder tool from the de novo assembly of 16 transcriptomes of cephalopods’ PSGs. Further details about database construction, as well as the scripts and command lines used to construct them, are deposited within Dataset_5 and Dataset_6. The data provided in this article will assist in unravelling the role of cephalopods’ PSGs in feeding strategies, toxins and AMP production.

ACS Style

Daniela Almeida; Dany Domínguez-Pérez; Ana Matos; Guillermin Agüero-Chapin; Yuselis Castaño; Vitor Vasconcelos; Alexandre Campos; Agostinho Antunes. Data Employed in the Construction of a Composite Protein Database for Proteogenomic Analyses of Cephalopods Salivary Apparatus. Data 2020, 5, 110 .

AMA Style

Daniela Almeida, Dany Domínguez-Pérez, Ana Matos, Guillermin Agüero-Chapin, Yuselis Castaño, Vitor Vasconcelos, Alexandre Campos, Agostinho Antunes. Data Employed in the Construction of a Composite Protein Database for Proteogenomic Analyses of Cephalopods Salivary Apparatus. Data. 2020; 5 (4):110.

Chicago/Turabian Style

Daniela Almeida; Dany Domínguez-Pérez; Ana Matos; Guillermin Agüero-Chapin; Yuselis Castaño; Vitor Vasconcelos; Alexandre Campos; Agostinho Antunes. 2020. "Data Employed in the Construction of a Composite Protein Database for Proteogenomic Analyses of Cephalopods Salivary Apparatus." Data 5, no. 4: 110.

Journal article
Published: 30 October 2020 in Antibiotics
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Cephalopods, successful predators, can use a mixture of substances to subdue their prey, becoming interesting sources of bioactive compounds. In addition to neurotoxins and enzymes, the presence of antimicrobial compounds has been reported. Recently, the transcriptome and the whole proteome of the Octopus vulgaris salivary apparatus were released, but the role of some compounds—e.g., histones, antimicrobial peptides (AMPs), and toxins—remains unclear. Herein, we profiled the proteome of the posterior salivary glands (PSGs) of O. vulgaris using two sample preparation protocols combined with a shotgun-proteomics approach. Protein identification was performed against a composite database comprising data from the UniProtKB, all transcriptomes available from the cephalopods’ PSGs, and a comprehensive non-redundant AMPs database. Out of the 10,075 proteins clustered in 1868 protein groups, 90 clusters corresponded to venom protein toxin families. Additionally, we detected putative AMPs clustered with histones previously found as abundant proteins in the saliva of O. vulgaris. Some of these histones, such as H2A and H2B, are involved in systemic inflammatory responses and their antimicrobial effects have been demonstrated. These results not only confirm the production of enzymes and toxins by the O. vulgaris PSGs but also suggest their involvement in the first line of defense against microbes.

ACS Style

Daniela Almeida; Dany Domínguez-Pérez; Ana Matos; Guillermin Agüero-Chapin; Hugo Osório; Vitor Vasconcelos; Alexandre Campos; Agostinho Antunes. Putative Antimicrobial Peptides of the Posterior Salivary Glands from the Cephalopod Octopus vulgaris Revealed by Exploring a Composite Protein Database. Antibiotics 2020, 9, 757 .

AMA Style

Daniela Almeida, Dany Domínguez-Pérez, Ana Matos, Guillermin Agüero-Chapin, Hugo Osório, Vitor Vasconcelos, Alexandre Campos, Agostinho Antunes. Putative Antimicrobial Peptides of the Posterior Salivary Glands from the Cephalopod Octopus vulgaris Revealed by Exploring a Composite Protein Database. Antibiotics. 2020; 9 (11):757.

Chicago/Turabian Style

Daniela Almeida; Dany Domínguez-Pérez; Ana Matos; Guillermin Agüero-Chapin; Hugo Osório; Vitor Vasconcelos; Alexandre Campos; Agostinho Antunes. 2020. "Putative Antimicrobial Peptides of the Posterior Salivary Glands from the Cephalopod Octopus vulgaris Revealed by Exploring a Composite Protein Database." Antibiotics 9, no. 11: 757.

Journal article
Published: 21 October 2020 in Toxins
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Microcystins (MCs) are hepatotoxins produced by some cyanobacteria. They are cyclic peptides that inhibit the serine/threonine protein phosphatases (PPs) PP1 and PP2A, especially PP2A. The inhibition of PP2A triggers a series of molecular events, which are responsible for most MC cytotoxic and genotoxic effects on animal cells. It is also known that MCs induce oxidative stress in cells due to the production of reactive oxygen species (ROS). However, a complete characterization of the toxic effects of MCs is still not accomplished. This study aimed to clarify additional molecular mechanisms involved in MC-LR toxicity, using Saccharomyces cerevisiae as eukaryotic model organism. First, a shotgun proteomic analysis of S. cerevisiae VL3 cells response to 1 nM, 10 nM, 100 nM, and 1 µM MC-LR was undertaken and compared to the control (cells not exposed to MC-LR). This analysis revealed a high number of proteins differentially expressed related with gene translation and DNA replication stress; oxidative stress; cell cycle regulation and carbohydrate metabolism. Inference of genotoxic effects of S. cerevisiae VL3 cells exposed to different concentrations of MC-LR were evaluated by analyzing the expression of genes Apn1, Apn2, Rad27, Ntg1, and Ntg2 (from the Base Excision Repair (BER) DNA repair system) using the Real-Time RT-qPCR technique. These genes displayed alterations after exposure to MC-LR, particularly the Apn1/Apn2/Rad27, pointing out effects of MC-LR in the Base Excision Repair system (BER). Overall, this study supports the role of oxidative stress and DNA replication stress as important molecular mechanisms of MC-LR toxicity. Moreover, this study showed that even at low-concentration, MC-LR can induce significant changes in the yeast proteome and in gene expression.

ACS Style

Elisabete Valério; Sara Barreiros; Sara Rodrigues; Maria Turkina; Vitor Vasconcelos; Alexandre Campos. New Insights in Saccharomyces cerevisiae Response to the Cyanotoxin Microcystin-LR, Revealed by Proteomics and Gene Expression. Toxins 2020, 12, 667 .

AMA Style

Elisabete Valério, Sara Barreiros, Sara Rodrigues, Maria Turkina, Vitor Vasconcelos, Alexandre Campos. New Insights in Saccharomyces cerevisiae Response to the Cyanotoxin Microcystin-LR, Revealed by Proteomics and Gene Expression. Toxins. 2020; 12 (10):667.

Chicago/Turabian Style

Elisabete Valério; Sara Barreiros; Sara Rodrigues; Maria Turkina; Vitor Vasconcelos; Alexandre Campos. 2020. "New Insights in Saccharomyces cerevisiae Response to the Cyanotoxin Microcystin-LR, Revealed by Proteomics and Gene Expression." Toxins 12, no. 10: 667.

Journal article
Published: 17 August 2020 in Toxins
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The application of natural compounds extracted from seaweeds is a promising eco-friendly alternative solution for harmful algae control in aquatic ecosystems. In the present study, the anti-cyanobacterial activity of three Moroccan marine macroalgae essential oils (EOs) was tested and evaluated on unicellular Microcystis aeruginosa cyanobacterium. Additionally, the possible anti-cyanobacterial response mechanisms were investigated by analyzing the antioxidant enzyme activities of M. aeruginosa cells. The results of EOs GC–MS analyses revealed a complex chemical composition, allowing the identification of 91 constituents. Palmitic acid, palmitoleic acid, and eicosapentaenoic acid were the most predominant compounds in Cystoseira tamariscifolia, Sargassum muticum, and Ulva lactuca EOs, respectively. The highest anti-cyanobacterial activity was recorded for Cystoseira tamariscifolia EO (ZI = 46.33 mm, MIC = 7.81 μg mL−1, and MBC = 15.62 μg mL−1). The growth, chlorophyll-a and protein content of the tested cyanobacteria were significantly reduced by C. tamariscifolia EO at both used concentrations (inhibition rate >67% during the 6 days test period in liquid media). Furthermore, oxidative stress caused by C. tamariscifolia EO on cyanobacterium cells showed an increase of the activities of superoxide dismutase (SOD) and catalase (CAT), and malondialdehyde (MDA) concentration was significantly elevated after 2 days of exposure. Overall, these experimental findings can open a promising new natural pathway based on the use of seaweed essential oils to the fight against potent toxic harmful cyanobacterial blooms (HCBs).

ACS Style

Soukaina El Amrani Zerrifi; Fatima El Khalloufi; Richard Mugani; Redouane El Mahdi; Ayoub Kasrati; Bouchra Soulaimani; Lillian Barros; Isabel Ferreira; Joana Amaral; Tiane Finimundy; Abdelaziz Abbad; Brahim Oudra; Alexandre Campos; Vitor Vasconcelos. Seaweed Essential Oils as a New Source of Bioactive Compounds for Cyanobacteria Growth Control: Innovative Ecological Biocontrol Approach. Toxins 2020, 12, 527 .

AMA Style

Soukaina El Amrani Zerrifi, Fatima El Khalloufi, Richard Mugani, Redouane El Mahdi, Ayoub Kasrati, Bouchra Soulaimani, Lillian Barros, Isabel Ferreira, Joana Amaral, Tiane Finimundy, Abdelaziz Abbad, Brahim Oudra, Alexandre Campos, Vitor Vasconcelos. Seaweed Essential Oils as a New Source of Bioactive Compounds for Cyanobacteria Growth Control: Innovative Ecological Biocontrol Approach. Toxins. 2020; 12 (8):527.

Chicago/Turabian Style

Soukaina El Amrani Zerrifi; Fatima El Khalloufi; Richard Mugani; Redouane El Mahdi; Ayoub Kasrati; Bouchra Soulaimani; Lillian Barros; Isabel Ferreira; Joana Amaral; Tiane Finimundy; Abdelaziz Abbad; Brahim Oudra; Alexandre Campos; Vitor Vasconcelos. 2020. "Seaweed Essential Oils as a New Source of Bioactive Compounds for Cyanobacteria Growth Control: Innovative Ecological Biocontrol Approach." Toxins 12, no. 8: 527.

Mini review
Published: 14 August 2020 in Archives of Microbiology
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Over the last decades, Harmful Cyanobacterial Blooms (HCBs) represent one of the most conspicuous hazards to human health in freshwater ecosystems, due to the uses of the water for drinking, recreation and aquaculture. Cyanobacteria are one of the main biological components in freshwater ecosystems and they may proliferate in nutrients rich ecosystems causing severe impacts at different levels. Therefore, several methods have been applied to control cyanobacterial proliferation, including physical, chemical and biological strategies. However, the application of those methods is generally not very efficient. Research on an eco-friendly alternative leading to the isolation of new bioactive compounds with strong impacts against harmful cyanobacteria is a need in the field of water environment protection. Thus, this paper aims to give an overview of harmful cyanobacterial blooms and reviews the state of the art of studying the activities of biological compounds obtained from plants, seaweeds and microorganisms in the cyanobacterial bloom control.

ACS Style

Soukaina El Amrani Zerrifi; Richard Mugani; El Mahdi Redouane; Fatima El Khalloufi; Alexandre Campos; Vitor Vasconcelos; Brahim Oudra. Harmful Cyanobacterial Blooms (HCBs): innovative green bioremediation process based on anti-cyanobacteria bioactive natural products. Archives of Microbiology 2020, 203, 31 -44.

AMA Style

Soukaina El Amrani Zerrifi, Richard Mugani, El Mahdi Redouane, Fatima El Khalloufi, Alexandre Campos, Vitor Vasconcelos, Brahim Oudra. Harmful Cyanobacterial Blooms (HCBs): innovative green bioremediation process based on anti-cyanobacteria bioactive natural products. Archives of Microbiology. 2020; 203 (1):31-44.

Chicago/Turabian Style

Soukaina El Amrani Zerrifi; Richard Mugani; El Mahdi Redouane; Fatima El Khalloufi; Alexandre Campos; Vitor Vasconcelos; Brahim Oudra. 2020. "Harmful Cyanobacterial Blooms (HCBs): innovative green bioremediation process based on anti-cyanobacteria bioactive natural products." Archives of Microbiology 203, no. 1: 31-44.

Review
Published: 31 July 2020 in Toxins
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Diarrhetic shellfish toxins (DSTs) are among the most prevalent marine toxins in Europe’s and in other temperate coastal regions. These toxins are produced by several dinoflagellate species; however, the contamination of the marine trophic chain is often attributed to species of the genus Dinophysis. This group of toxins, constituted by okadaic acid (OA) and analogous molecules (dinophysistoxins, DTXs), are highly harmful to humans, causing severe poisoning symptoms caused by the ingestion of contaminated seafood. Knowledge on the mode of action and toxicology of OA and the chemical characterization and accumulation of DSTs in seafood species (bivalves, gastropods and crustaceans) has significantly contributed to understand the impacts of these toxins in humans. Considerable information is however missing, particularly at the molecular and metabolic levels involving toxin uptake, distribution, compartmentalization and biotransformation and the interaction of DSTs with aquatic organisms. Recent contributions to the knowledge of DSTs arise from transcriptomics and proteomics research. Indeed, OMICs constitute a research field dedicated to the systematic analysis on the organisms’ metabolisms. The methodologies used in OMICs are also highly effective to identify critical metabolic pathways affecting the physiology of the organisms. In this review, we analyze the main contributions provided so far by OMICs to DSTs research and discuss the prospects of OMICs with regard to the DSTs toxicology and the significance of these toxins to public health, food safety and aquaculture.

ACS Style

Alexandre Campos; Marisa Freitas; André De Almeida; José Martins; Dany Domínguez-Pérez; Hugo Osório; Vitor Vasconcelos; Pedro Reis Costa. OMICs Approaches in Diarrhetic Shellfish Toxins Research. Toxins 2020, 12, 493 .

AMA Style

Alexandre Campos, Marisa Freitas, André De Almeida, José Martins, Dany Domínguez-Pérez, Hugo Osório, Vitor Vasconcelos, Pedro Reis Costa. OMICs Approaches in Diarrhetic Shellfish Toxins Research. Toxins. 2020; 12 (8):493.

Chicago/Turabian Style

Alexandre Campos; Marisa Freitas; André De Almeida; José Martins; Dany Domínguez-Pérez; Hugo Osório; Vitor Vasconcelos; Pedro Reis Costa. 2020. "OMICs Approaches in Diarrhetic Shellfish Toxins Research." Toxins 12, no. 8: 493.

Journal article
Published: 30 July 2020 in Biomolecules
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The development of alternative ecological and effective antifouling technologies is still challenging. Synthesis of nature-inspired compounds has been exploited, given the potential to assure commercial supplies of potential ecofriendly antifouling agents. In this direction, the antifouling activity of a series of nineteen synthetic small molecules, with chemical similarities with natural products, were exploited in this work. Six (4, 5, 7, 10, 15 and 17) of the tested xanthones showed in vivo activity toward the settlement of Mytilus galloprovincialis larvae (EC50: 3.53–28.60 µM) and low toxicity to this macrofouling species (LC50 > 500 µM and LC50/EC50: 17.42–141.64), and two of them (7 and 10) showed no general marine ecotoxicity (Artemia salina mortality) after 48 h of exposure. Regarding the mechanism of action in mussel larvae, the best performance compounds 4 and 5 might be acting by the inhibition of acetylcholinesterase activity (in vitro and in silico studies), while 7 and 10 showed specific targets (proteomic studies) directly related with the mussel adhesive structure (byssal threads), given by the alterations in the expression of Mytilus collagen proteins (PreCols) and proximal thread proteins (TMPs). A quantitative structure-activity relationship (QSAR) model was built with predictive capacity to enable speeding the design of new potential active compounds.

ACS Style

Joana Almeida; Andreia Palmeira; Alexandre Campos; Isabel Cunha; Micaela Freitas; Aldo Felpeto; Maria Turkina; Vitor Vasconcelos; Madalena Pinto; Marta Correia-Da-Silva; Maria Emília Sousa. Structure-Antifouling Activity Relationship and Molecular Targets of Bio-Inspired(thio)xanthones. Biomolecules 2020, 10, 1126 .

AMA Style

Joana Almeida, Andreia Palmeira, Alexandre Campos, Isabel Cunha, Micaela Freitas, Aldo Felpeto, Maria Turkina, Vitor Vasconcelos, Madalena Pinto, Marta Correia-Da-Silva, Maria Emília Sousa. Structure-Antifouling Activity Relationship and Molecular Targets of Bio-Inspired(thio)xanthones. Biomolecules. 2020; 10 (8):1126.

Chicago/Turabian Style

Joana Almeida; Andreia Palmeira; Alexandre Campos; Isabel Cunha; Micaela Freitas; Aldo Felpeto; Maria Turkina; Vitor Vasconcelos; Madalena Pinto; Marta Correia-Da-Silva; Maria Emília Sousa. 2020. "Structure-Antifouling Activity Relationship and Molecular Targets of Bio-Inspired(thio)xanthones." Biomolecules 10, no. 8: 1126.

Journal article
Published: 13 July 2020 in Marine Drugs
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Ascidians are marine invertebrates associated with diverse microbial communities, embedded in their tunic, conferring special ecological and biotechnological relevance to these model organisms used in evolutionary and developmental studies. Next-generation sequencing tools have increased the knowledge of ascidians’ associated organisms and their products, but proteomic studies are still scarce. Hence, we explored the tunic of three ascidian species using a shotgun proteomics approach. Proteins extracted from the tunic of Ciona sp., Molgula sp., and Microcosmus sp. were processed using a nano LC-MS/MS system (Ultimate 3000 liquid chromatography system coupled to a Q-Exactive Hybrid Quadrupole-Orbitrap mass spectrometer). Raw data was searched against UniProtKB – the Universal Protein Resource Knowledgebase (Bacteria and Metazoa section) using Proteome Discoverer software. The resulting proteins were merged with a non-redundant Antimicrobial Peptides (AMPs) database and analysed with MaxQuant freeware. Overall, 337 metazoan and 106 bacterial proteins were identified being mainly involved in basal metabolism, cytoskeletal and catalytic functions. 37 AMPs were identified, most of them attributed to eukaryotic origin apart from bacteriocins. These results and the presence of “Biosynthesis of antibiotics” as one of the most highlighted pathways revealed the tunic as a very active tissue in terms of bioactive compounds production, giving insights on the interactions between host and associated organisms. Although the present work constitutes an exploratory study, the approach employed revealed high potential for high-throughput characterization and biodiscovery of the ascidians’ tunic and its microbiome.

ACS Style

Ana Matos; Dany Domínguez-Pérez; Daniela Almeida; Guillermin Agüero-Chapin; Alexandre Campos; Hugo Osório; Vitor Vasconcelos; Agostinho Antunes. Shotgun Proteomics of Ascidians Tunic Gives New Insights on Host–Microbe Interactions by Revealing Diverse Antimicrobial Peptides. Marine Drugs 2020, 18, 362 .

AMA Style

Ana Matos, Dany Domínguez-Pérez, Daniela Almeida, Guillermin Agüero-Chapin, Alexandre Campos, Hugo Osório, Vitor Vasconcelos, Agostinho Antunes. Shotgun Proteomics of Ascidians Tunic Gives New Insights on Host–Microbe Interactions by Revealing Diverse Antimicrobial Peptides. Marine Drugs. 2020; 18 (7):362.

Chicago/Turabian Style

Ana Matos; Dany Domínguez-Pérez; Daniela Almeida; Guillermin Agüero-Chapin; Alexandre Campos; Hugo Osório; Vitor Vasconcelos; Agostinho Antunes. 2020. "Shotgun Proteomics of Ascidians Tunic Gives New Insights on Host–Microbe Interactions by Revealing Diverse Antimicrobial Peptides." Marine Drugs 18, no. 7: 362.

Research article
Published: 02 July 2020 in Biofouling
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Cyanobacteria promote marine biofouling with significant impacts. A qualitative proteomic analysis, by LC-MS/MS, of planktonic and biofilm cells from two cyanobacteria was performed. Biofilms were formed on glass and perspex at two relevant hydrodynamic conditions for marine environments (average shear rates of 4 s−1 and 40 s−1). For both strains and surfaces, biofilm development was higher at 4 s−1. Biofilm development of Nodosilinea sp. LEGE 06145 was substantially higher than Nodosilinea sp. LEGE 06119, but no significant differences were found between surfaces. Overall, 377 and 301 different proteins were identified for Nodosilinea sp. LEGE 06145 and Nodosilinea sp. LEGE 06119. Differences in protein composition were more noticeable in biofilms formed under different hydrodynamic conditions than in those formed on different surfaces. Ribosomal and photosynthetic proteins were identified in most conditions. The characterization performed gives new insights into how shear rate and surface affect the planktonic to biofilm transition, from a structural and proteomics perspective.

ACS Style

Maria João Leal Romeu; Dany Domínguez-Pérez; Daniela Almeida; João Morais; Alexandre Campos; Vítor Vasconcelos; Filipe J. M. Mergulhão. Characterization of planktonic and biofilm cells from two filamentous cyanobacteria using a shotgun proteomic approach. Biofouling 2020, 36, 631 -645.

AMA Style

Maria João Leal Romeu, Dany Domínguez-Pérez, Daniela Almeida, João Morais, Alexandre Campos, Vítor Vasconcelos, Filipe J. M. Mergulhão. Characterization of planktonic and biofilm cells from two filamentous cyanobacteria using a shotgun proteomic approach. Biofouling. 2020; 36 (6):631-645.

Chicago/Turabian Style

Maria João Leal Romeu; Dany Domínguez-Pérez; Daniela Almeida; João Morais; Alexandre Campos; Vítor Vasconcelos; Filipe J. M. Mergulhão. 2020. "Characterization of planktonic and biofilm cells from two filamentous cyanobacteria using a shotgun proteomic approach." Biofouling 36, no. 6: 631-645.

Journal article
Published: 05 April 2020 in International Journal of Molecular Sciences
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Adhesive secretion has a fundamental role in barnacles’ survival, keeping them in an adequate position on the substrate under a variety of hydrologic regimes. It arouses special interest for industrial applications, such as antifouling strategies, underwater industrial and surgical glues, and dental composites. This study was focused on the goose barnacle Pollicipes pollicipes adhesion system, a species that lives in the Eastern Atlantic strongly exposed intertidal rocky shores and cliffs. The protein composition of P. pollicipes cement multicomplex and cement gland was quantitatively studied using a label-free LC-MS high-throughput proteomic analysis, searched against a custom transcriptome-derived database. Overall, 11,755 peptide sequences were identified in the gland while 2880 peptide sequences were detected in the cement, clustered in 1616 and 1568 protein groups, respectively. The gland proteome was dominated by proteins of the muscle, cytoskeleton, and some uncharacterized proteins, while the cement was, for the first time, reported to be composed by nearly 50% of proteins that are not canonical cement proteins, mainly unannotated proteins, chemical cues, and protease inhibitors, among others. Bulk adhesive proteins accounted for one-third of the cement proteome, with CP52k being the most abundant. Some unannotated proteins highly expressed in the proteomes, as well as at the transcriptomic level, showed similar physicochemical properties to the known surface-coupling barnacle adhesive proteins while the function of the others remains to be discovered. New quantitative and qualitative clues are provided to understand the diversity and function of proteins in the cement of stalked barnacles, contributing to the whole adhesion model in Cirripedia.

ACS Style

Dany Domínguez-Pérez; Daniela Almeida; Josef Wissing; André M. Machado; Lothar Jänsch; Luís Filipe Castro; Agostinho Antunes; Vitor Vasconcelos; Alexandre Campos; Isabel Cunha. The Quantitative Proteome of the Cement and Adhesive Gland of the Pedunculate Barnacle, Pollicipes pollicipes. International Journal of Molecular Sciences 2020, 21, 2524 .

AMA Style

Dany Domínguez-Pérez, Daniela Almeida, Josef Wissing, André M. Machado, Lothar Jänsch, Luís Filipe Castro, Agostinho Antunes, Vitor Vasconcelos, Alexandre Campos, Isabel Cunha. The Quantitative Proteome of the Cement and Adhesive Gland of the Pedunculate Barnacle, Pollicipes pollicipes. International Journal of Molecular Sciences. 2020; 21 (7):2524.

Chicago/Turabian Style

Dany Domínguez-Pérez; Daniela Almeida; Josef Wissing; André M. Machado; Lothar Jänsch; Luís Filipe Castro; Agostinho Antunes; Vitor Vasconcelos; Alexandre Campos; Isabel Cunha. 2020. "The Quantitative Proteome of the Cement and Adhesive Gland of the Pedunculate Barnacle, Pollicipes pollicipes." International Journal of Molecular Sciences 21, no. 7: 2524.

Journal article
Published: 20 March 2020 in Toxins
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Toxic cyanobacterial blooms are a major contaminant in inland aquatic ecosystems. Furthermore, toxic blooms are carried downstream by rivers and waterways to estuarine and coastal ecosystems. Concerning marine and estuarine animal species, very little is known about how these species are affected by the exposure to freshwater cyanobacteria and cyanotoxins. So far, most of the knowledge has been gathered from freshwater bivalve molluscs. This work aimed to infer the sensitivity of the marine mussel Mytilus galloprovincialis to single as well as mixed toxic cyanobacterial cultures and the underlying molecular responses mediated by toxic cyanobacteria. For this purpose, a mussel exposure experiment was outlined with two toxic cyanobacteria species, Microcystis aeruginosa and Chrysosporum ovalisporum at 1 × 105 cells/mL, resembling a natural cyanobacteria bloom. The estimated amount of toxins produced by M. aeruginosa and C. ovalisporum were respectively 0.023 pg/cell of microcystin-LR (MC-LR) and 7.854 pg/cell of cylindrospermopsin (CYN). After 15 days of exposure to single and mixed cyanobacteria, a depuration phase followed, during which mussels were fed only non-toxic microalga Parachlorella kessleri. The results showed that the marine mussel is able to filter toxic cyanobacteria at a rate equal or higher than the non-toxic microalga P. kessleri. Filtration rates observed after 15 days of feeding toxic microalgae were 1773.04 mL/ind.h (for M. aeruginosa), 2151.83 mL/ind.h (for C. ovalisporum), 1673.29 mL/ind.h (for the mixture of the 2 cyanobacteria) and 2539.25 mL/ind.h (for the non-toxic P. kessleri). Filtering toxic microalgae in combination resulted in the accumulation of 14.17 ng/g dw MC-LR and 92.08 ng/g dw CYN. Other physiological and biochemical endpoints (dry weight, byssus production, total protein and glycogen) measured in this work did not change significantly in the groups exposed to toxic cyanobacteria with regard to control group, suggesting that mussels were not affected with the toxic microalgae. Nevertheless, proteomics revealed changes in metabolism of mussels related to diet, specially evident in those fed on combined cyanobacteria. Changes in metabolic pathways related with protein folding and stabilization, cytoskeleton structure, and gene transcription/translation were observed after exposure and feeding toxic cyanobacteria. These changes occur in vital metabolic processes and may contribute to protect mussels from toxic effects of the toxins MC-LR and CYN.

ACS Style

Flavio Oliveira; Leticia Diez-Quijada; Maria V. Turkina; João Morais; Aldo Barreiro Felpeto; Joana Azevedo; Angeles Jos; Ana M. Camean; Vitor Vasconcelos; José Carlos Martins; Alexandre Campos. Physiological and Metabolic Responses of Marine Mussels Exposed to Toxic Cyanobacteria Microcystis aeruginosa and Chrysosporum ovalisporum. Toxins 2020, 12, 196 .

AMA Style

Flavio Oliveira, Leticia Diez-Quijada, Maria V. Turkina, João Morais, Aldo Barreiro Felpeto, Joana Azevedo, Angeles Jos, Ana M. Camean, Vitor Vasconcelos, José Carlos Martins, Alexandre Campos. Physiological and Metabolic Responses of Marine Mussels Exposed to Toxic Cyanobacteria Microcystis aeruginosa and Chrysosporum ovalisporum. Toxins. 2020; 12 (3):196.

Chicago/Turabian Style

Flavio Oliveira; Leticia Diez-Quijada; Maria V. Turkina; João Morais; Aldo Barreiro Felpeto; Joana Azevedo; Angeles Jos; Ana M. Camean; Vitor Vasconcelos; José Carlos Martins; Alexandre Campos. 2020. "Physiological and Metabolic Responses of Marine Mussels Exposed to Toxic Cyanobacteria Microcystis aeruginosa and Chrysosporum ovalisporum." Toxins 12, no. 3: 196.

Journal article
Published: 21 February 2020 in Environmental Research
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The aim of this study was to optimize the extraction conditions of Microcystin-LR (MC-LR), Microcystin-RR (MC-RR), Microcystin-YR (MC-YR) and Cylindrospermopsin (CYN) simultaneously from mussels by using response surface methodology (RSM) and to validate the method by a dual solid phase extraction (SPE) system combined with ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The optimal parameters were: 90% MeOH (% v/v) for the extraction, a solvent/sample ratio of 75 and 15% MeOH in the extract before loading onto SPE. Mussels were spiked at 10; 37.5 and 75 ng g−1 fresh weight (f.w) of the 4 toxins, showing linear ranges of 0.5–75 ng g−1 f.w; low values for the limits of detection (0.01–0.39 ng g−1 f.w.) and quantification (0.23–0.40 ng g−1 f.w.); acceptable recoveries (70.37–114.03%) and relative standard deviation (%RSDIP) values (2.61–13.73%). The method was successfully applied to edible mussels exposed to cyanobacterial extracts under laboratory conditions, and it could allow the monitoring of these cyanotoxins in environmental mussel samples.

ACS Style

Leticia Díez-Quijada Jiménez; Remedios Guzmán-Guillén; Giorgiana M. Cătunescu; Alexandre Campos; Vitor Vasconcelos; Ángeles Jos; Ana M. Cameán. A new method for the simultaneous determination of cyanotoxins (Microcystins and Cylindrospermopsin) in mussels using SPE-UPLC-MS/MS. Environmental Research 2020, 185, 109284 .

AMA Style

Leticia Díez-Quijada Jiménez, Remedios Guzmán-Guillén, Giorgiana M. Cătunescu, Alexandre Campos, Vitor Vasconcelos, Ángeles Jos, Ana M. Cameán. A new method for the simultaneous determination of cyanotoxins (Microcystins and Cylindrospermopsin) in mussels using SPE-UPLC-MS/MS. Environmental Research. 2020; 185 ():109284.

Chicago/Turabian Style

Leticia Díez-Quijada Jiménez; Remedios Guzmán-Guillén; Giorgiana M. Cătunescu; Alexandre Campos; Vitor Vasconcelos; Ángeles Jos; Ana M. Cameán. 2020. "A new method for the simultaneous determination of cyanotoxins (Microcystins and Cylindrospermopsin) in mussels using SPE-UPLC-MS/MS." Environmental Research 185, no. : 109284.

Journal article
Published: 11 February 2020 in Aquatic Toxicology
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Despite the widespread use of nanoparticles (NPs), there are still major gaps of knowledge regarding the impact of nanomaterials in the environment and aquatic animals. The present work aimed to study the effects of 7 and 40 nm gold nanoparticles (AuNPs) – citrate and polyvinylpyrrolidone (PVP) coated – on the liver proteome of the estuarine/marine fish gilthead seabream (Sparus aurata). After 96 h, exposure to AuNP elicited alterations on the abundance of 26 proteins, when compared to the control group. AuNPs differentially affected several metabolic pathways in S. aurata liver cells. Among the affected proteins were those related to cytoskeleton and cell structure, gluconeogenesis, amino acids metabolism and several processes related to protein activity (protein synthesis, catabolism, folding and transport). The increased abundance of proteins associated with energy metabolism (ATP synthase subunit beta), stress response (94 kDa glucose-regulated protein) and cytoskeleton structure (actins and tubulins) may represent the first signs of cellular oxidative stress induced by AuNPs. Although higher gold accumulation was found in the liver of S. aurata exposed to 7 nm PVP-AuNPs, the 7 nm cAuNPs were more bioactive, inducing more effects in liver proteome. Gold accumulated more in the spleen than in the other assessed tissues of S. aurata exposed to AuNPs, highlighting its potential role on the elimination of these NPs.

ACS Style

A. Barreto; Ana Carvalho; Alexandre Campos; H. Osório; Edgar Pinto; Agostinho Almeida; T. Trindade; A.M.V.M. Soares; K. Hylland; Susana Loureiro; Miguel Oliveira. Effects of gold nanoparticles in gilthead seabream—A proteomic approach. Aquatic Toxicology 2020, 221, 105445 .

AMA Style

A. Barreto, Ana Carvalho, Alexandre Campos, H. Osório, Edgar Pinto, Agostinho Almeida, T. Trindade, A.M.V.M. Soares, K. Hylland, Susana Loureiro, Miguel Oliveira. Effects of gold nanoparticles in gilthead seabream—A proteomic approach. Aquatic Toxicology. 2020; 221 ():105445.

Chicago/Turabian Style

A. Barreto; Ana Carvalho; Alexandre Campos; H. Osório; Edgar Pinto; Agostinho Almeida; T. Trindade; A.M.V.M. Soares; K. Hylland; Susana Loureiro; Miguel Oliveira. 2020. "Effects of gold nanoparticles in gilthead seabream—A proteomic approach." Aquatic Toxicology 221, no. : 105445.