Changes in the bacterial spectrum of cabbage heads after storage under commonly used storage conditions were examined in this study. Cabbage seeds (Brassica oleracea var. capitata L.) were artificially inoculated with X. campestris pv. campestris (Xcc), a serious pathogen of cruciferous plants causing black rot. Isolation of bacterial cultures from Xcc-inoculated and non-inoculated cabbage heads were carried out in two time points—at the day of harvest and after four months of storage. According to our previous research and literature reports, the most frequent genera of bacteria were chosen for PCR testing, i.e., Bacillus cereus group, Bacillus subtilis group, Pseudomonas sp., and X. campestris pv. campestris. A few of the obtained bacterial cultures were negative for the four above-mentioned species. In those, other bacteria were identified by 16S rRNA sequencing. In both Xcc-inoculated and non-inoculated cabbage heads, changes of the bacterial spectrum over time were observed. The severity of Xcc infection of heads increased after four months of storage. Bacillus species represented the most frequently occurring bacterial genus. The presence of the Bacillus subtilis group increased significantly after storage in non-inoculated cabbage heads. The minor part of the other genera identified by sequencing in the first sampling were not detected in the stored cabbage heads. This was associated with a possible antagonistic behavior of Pseudomonas sp. and Bacillus sp.

Viticulture, as a large part of the agriculture sector of the South Moravian Region, represents significant erosion-prone land use in which soils face various agronomic issues, such as poor organic carbon levels, erosion, and fertility loss. Service crops providing a so-called ecosystem service can reduce erosion and runoff, regulate pests and weeds and increase soil organic matter and fertility. However, these crops may generate some disservices, such as water and nutrient competition; and thus, it is important for winegrowers to find applicable options for service crops depending on local soil, climate conditions, and the expected service. Inter-row management in the South Moravian Region varies from bare soils to grass cover to different types of cover with herbaceous (flowering) species. A total of 113 vineyard sites were evaluated during the years 2016 and 2017. This study presents the actual state of inter-row management in vineyards and comparison within six wine-growing regions. A two-year evaluation shows significant differences in prevalent greening management between regions. Bare soil in vineyards, the most erosion-prone vineyard floor management, appear from 10% (e.g., Bzenec, Valtice) to 19% (e.g., Mikulov, V. Bílovice) of vineyard area within evaluated regions. Bare soil management is mostly used in new plantations to reduce water and nutrient competition; however, the erosion and the runoff rates are generally higher on this variant compared to other types of cover crop management, especially on slopes. Although, alternate greening is the most used type occurring from 50% to 74% of vineyards area in five of the six selected regions, the type of inter-row vegetation differs considerably. While in Bzenec and Mikulov there is a higher appearance of herbaceous cover with native species in later succession stages, in Velké Bílovice and Valtice grass cover and commercial plant mixtures are more frequent. Knowledge current stage can be useful for planning new plantation or anti-erosion measures.

Serendipita indica, a mutualistic root endophytic fungus, has gained attention for its potential to enhance plant health and resistance to various stresses. This study investigated the impact of S. indica (strain DSM 11827) on plant growth promotion and the management of black rot disease. This is a devastating bacterial ailment caused by Xanthomonas campestris pv. campestris, which affects cruciferous crops worldwide. The experiment was conducted under sterile conditions in a phytotron for 10 weeks. It involved the substrate and seed inoculation of S. indica in a cabbage crop. The findings suggested that S. indica establishes mutualistic relationships with cruciferous plants, positively influencing their growth while simultaneously reducing their susceptibility to black rot disease. Plant morphological and physiological parameters were enhanced by S. indica application. Additionally, bio stress markers were also enhanced in response to black rot disease. Moreover, disease severity was reduced by 27.9% and 18.8% in the substrate and seeds treated with S. indica, respectively. However, our findings did not report any antagonistic effect between S. indica and two pathogens, i.e., Xanthomonas campestris pv. campestris and Alternaria brassicicola under the in vitro test, suggesting that the suppression of black rot disease in cabbage seedlings was induced indirectly by S. indica. This study, therefore, underscores the promising prospect of utilizing S. indica to promote crop productivity and combat the destructive impact of black rot disease in cruciferous plants, contributing to more sustainable and resilient agriculture.

Introduction: Smart management in crop cultivation is increasingly supported by application of arbuscular mycorrhizal fungi (AMF) and plant growth-promoting microorganisms (PGPM), which sustain soil fertility and plant performance. The aim of this study was the evaluation of the effects of consortia composed of (Claroideoglomus claroideum BEG96, Claroideoglomus etunicatum BEG92, Funneliformis geosporum BEG199, Funneliformis mosseae BEG 95, and Rhizophagus irregularis BEG140) and PGPM (Azospirillum brasilense – AZ, or Saccharothrix sp. – S) on onion cultivated in growing media with a composition corresponding to a degraded soil. Methods: Three types of substrate formulations were used, with peat:sand ratios of 50:50, 70:30, 100:0 (v:v). The analysis of substrate parameters crucial for its fertility (pH, salinity, sorption complex capacity, and elements’ content) and characteristics reflecting onion seedlings’ performance (fresh weight, stress biomarkers, and elements’ content) was performed. Results: AMF colonized onion roots in all treatments, showing increasing potential to form intercellular structures in the substrates rich in organic matter. Additionally, co-inoculation with PGPM microorganisms accelerated arbuscular mycorrhiza establishment. Increased antioxidant activity and glutathione peroxidase (GPOX) activity of onion roots sampled from the formulations composed of peat and sand in the ratio of 100:0, inoculated with AMF+S, and positive correlation between GPOX, fresh weight and antioxidant activity of onion roots reflected the successful induction of plant acclimatization response. Total phenols content was the highest in roots and leaves of onion grown in substrates with 70:30 peat:sand ratio, and, in the case of roots, it was correlated with AMF colonization parameters but not with antioxidant activity.Discussion: AMF and PGPM efficiency in supporting onion growth should be linked to the increased onion root system capacity in mineral salts absorption, resulting in more efficient aboveground biomass production. AMF and PGPM consortia were effective in releasing minerals to soluble fraction in substrates rich in organic matter, making elements available for uptake by onion root system, though this phenomenon depended on the PGPM species. Microorganism consortia enhanced onion seedlings’ performance also in substrates with lower content of organic carbon through plant biofertilization and phytostimulation

Increasing vulnerability of crops to pests and diseases, problems with soil erosion, a decline in biodiversity and a number of other negative impacts caused by agricultural intensification and monocultural production have been the subjects of many studies in recent decades. Today, cover cropping has become a promising practice to defuse these negative impacts, and it is emerging in many wine-producing regions, including the Czech Republic. However, the importance of permanent natural and semi-natural habitats in agricultural production should not be neglected. In this study, the effect of adjacent non-crop vegetation on plant and insect diversity was evaluated. The highest plant species richness of inter-row vegetation was found in vineyards with a high proportion (>40%) of non-crop vegetation within a 500-m radius. Regarding the agricultural impact of inter-row vegetation, the high proportion of non-crop vegetation could have been related to the higher presence of opportunistic and non-harmful weeds, compared with the presence of dangerous weed species. The number of insect families present in inter-rows was probably affected more by the vegetation coverage rate than by the proportion of adjacent non-crop vegetation. However, the occurrence of the Hymenoptera species, often representing beneficial organisms, was related to localities with a high proportion of adjacent non-crop vegetation.

Symbiotic interaction between plants and microorganisms in the rhizosphere is an important factor affecting plant growth and fitness. Arbuscular mycorrhiza fungi symbiosis increases resistance of the plants to stress factors, including pathogens. Tomato brown rugose fruit virus (ToBRFV) is an important destructive virus damaging tomatoes and peppers with losses that can reach 100%. It is listed on the list of current quarantine organisms in the Czech Republic. The aim of this study was to evaluate influence of root colonization with Funneliformis mosseae or/and Azospirillum brasilense on ToBRFV symptoms and viral titre reduction. Plants treated with arbuscular mycorhizal fungi (AMF) had lower symptom emergence after 14 dpi, however there was no difference in symptoms emergence after 21 dpi within all treatments. The highest colonization intensity by Funneliformis mosseae was detected in ToBRFV negative plants treated with both AMF and Azospirillum (AZO) and the lowest in ToBRFV positive plants with the same treatment (AMF + AZO). Colonization intensity of Azospirillum brasilense in all treated variants went from 20% to 41%. Results suggest that the combination of those two beneficial microorganisms in ToBRFV-infected plants negatively affected AMF colonization.