Plant growth regulators (PRG)-assisted phytoremediation is a technique that could enhance the yield of heavy metal accumulation in plant tissues. So far, a small number of experiments have helped identify three groups of plant hormones that ma...

Background: Humic substances (HS) are compounds with a complicated structure, present in the humus soil layer, water, lake sediments, peat, brown coal and shales. Due to their similar physicochemical properties to DNA, they may have an adverse effect on the subsequent use of the isolated material. The main aim of this research was to examine the effect of HS on DNA isolation depending on the soil type and land use, taking into account the spectroscopic full characteristics of HS fractions. Methods: The research was conducted on eight types of soil sample. Soils represented the most important Soil Reference Groups for temperate climates: Fluvisols, Regosols, Cambisols, Arenosols, Histosols and Luvisols. Soil samples were also collected from areas diversified in terms of use: arable land, grassland and forest. The extraction of HS fractions was performed using the procedure recommended by the International HS Society. The fractional composition of HS was characterized by UV–Vis and fluorescence methods. Soil DNA is extracted by direct cell lysis in the using a CTAB-based method with a commonly-used commercial soil DNA isolation kit. The basis for assessing the quantity and quality of extracted DNA was the Polymerase chain reaction (PCR) reaction since the analysis of soil DNA often relies on the use of PCR to study soil microorganisms. Results: Based on the results, it can be concluded that in the presence of a high concentration of HS, the isolated DNA was low quality and the additional purification procedure was necessary. Despite the differentiation of the internal structure of HS fractions, the decisive factor in the efficiency of DNA isolation from soil samples was the total carbon content in HS. Reduced DNA yields can significantly constrain PCR detection limits to levels inadequate for metagenomic analysis, especially from humus-rich soils.

Sewage sludge (SS) has been connected to a variety of global environmental problems. Assessing the risk of various disposal techniques can be quite useful in recommending appropriate management. The preparation of sewage sludge biochar (SSB) and its impacts on soil characteristics, plant health, nutrient leaching, and greenhouse gas emissions (GHGs) are critically reviewed in this study. Comparing the features of SSB obtained at various pyrolysis temperatures revealed changes in its elemental content. Lower hydrogen/carbon ratios in SSB generated at higher pyrolysis temperatures point to the existence of more aromatic carbon molecules. Additionally, the preparation of SSB has an increased ash content, a lower yield, and a higher surface area as a result of the rise in pyrolysis temperature. The worldwide potential of SS output and CO₂-equivalent emissions in 2050 were predicted as factors of global population and common disposal management in order to create a futuristic strategy and cope with the quantity of abundant global SS. According to estimations, the worldwide SS output and associated CO₂-eq emissions were around 115 million tons dry solid (Mt DS) and 14,139 teragrams (Tg), respectively, in 2020. This quantity will rise to about 138 Mt DS sewage sludge and 16985 Tg CO₂-eq emissions in 2050, a 20% increase. In this regard, developing and populous countries may support economic growth by utilizing low-cost methods for producing biochar and employing it in local agriculture. To completely comprehend the benefits and drawbacks of SSB as a soil supplement, further study on long-term field applications of SSB is required.

Chemical nitrogen (N) fertilizers are regarded as one of the environmental contaminants in addition to the necessity for fossil sources for their production. Conversely, it is impossible to neglect the supply of nitrogen needed as one of the essential ingredients for plant function. For organic agriculture, it is crucial to use alternative fertilizer management to reduce the harmful impacts and production costs of chemical fertilizers. In a one-year pot experiment, nitrate (NO⁻₃) leaching and nitrogen efficiency of wheat were examined in relation to biochar (B) mixed with urea (U), legume residues (L), and azocompost (A), which represent chemical, green, and biological sources of N-fertilizers, respectively. Control (no biochar, no fertilizer), U (46 kg ha⁻¹), A (5 t ha⁻¹), L (5 t ha⁻¹), B (10 t ha⁻¹), UB, AB, and LB were the experimental treatments. Grain yield of wheat was enhanced by 337% and 312% with UB and UL, respectively. The LB produced the highest grain N yield, with a rise of 8.8 times over the control. L had the highest N-use efficiency, with an increase of 149% over the control. The highest N-harvest index and N-recovery efficiency were obtained by using LB, with values of 91 and 70 %, respectively. Nitrate leaching occurred in the following order: U > Control ≥ A ≥ L > UB > AB ≥ LB > B. Nitrogen is retained for the plant in the extensive specific surface of biochar when N-fertilizers are used in conjunction with them. This not only improves N-efficiency but also minimizes nitrogen loss through leaching. Additionally, the soil can benefit from the addition of leguminous organic fertilizer in a similar way as to urea fertilizer in terms of increasing wheat grain yield, particularly when combined with biochar.