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Jiasen Cheng
Hubei Key Laboratory of Plant Pathology, Huazhong Agricultural University, Wuhan 430070, China

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Journal article
Published: 22 June 2021 in Journal of Fungi
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The infection by a single-stranded DNA virus, Sclerotinia sclerotiorum hypovirulence-associated DNA virus 1 (SsHADV-1), causes hypovirulence, a reduced growth rate, and other colony morphological changes in its host Sclerotinia sclerotiorum strain DT-8. However, the mechanisms of the decline are still unclear. Using digital RNA sequencing, a transcriptome analysis was conducted to elucidate the phenotype-related genes with expression changes in response to SsHADV-1 infection. A total of 3110 S. sclerotiorum differentially expressed genes (DEGs) were detected during SsHADV-1 infection, 1741 of which were up-regulated, and 1369 were down-regulated. The identified DEGs were involved in several important pathways. DNA replication, DNA damage response, carbohydrate and lipid metabolism, ribosomal assembly, and translation were the affected categories in S. sclerotiorum upon SsHADV-1 infection. Moreover, the infection of SsHADV-1 also suppressed the expression of antiviral RNA silencing and virulence factor genes. These results provide further detailed insights into the effects of SsHADV-1 infection on the whole genome transcription in S. sclerotiorum.

ACS Style

Zheng Qu; Yanping Fu; Yang Lin; Zhenzhen Zhao; Xuekun Zhang; Jiasen Cheng; Jiatao Xie; Tao Chen; Bo Li; Daohong Jiang. Transcriptional Responses of Sclerotinia sclerotiorum to the Infection by SsHADV-1. Journal of Fungi 2021, 7, 493 .

AMA Style

Zheng Qu, Yanping Fu, Yang Lin, Zhenzhen Zhao, Xuekun Zhang, Jiasen Cheng, Jiatao Xie, Tao Chen, Bo Li, Daohong Jiang. Transcriptional Responses of Sclerotinia sclerotiorum to the Infection by SsHADV-1. Journal of Fungi. 2021; 7 (7):493.

Chicago/Turabian Style

Zheng Qu; Yanping Fu; Yang Lin; Zhenzhen Zhao; Xuekun Zhang; Jiasen Cheng; Jiatao Xie; Tao Chen; Bo Li; Daohong Jiang. 2021. "Transcriptional Responses of Sclerotinia sclerotiorum to the Infection by SsHADV-1." Journal of Fungi 7, no. 7: 493.

Research article
Published: 14 May 2021 in Environmental Microbiology
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Rice sheath blight caused by Rhizoctonia solani is the major disease of rice that seriously threatens food security worldwide. Efficient and eco‐friendly biological approaches are urgently needed since no resistant cultivars are available. In this study, fallow and paddy soils were initially subjected to microbiome analyses, and the results showed that Talaromyces spp. were significantly more abundant in the paddy soil, while Trichoderma spp. were more abundant in the fallow soil, suggesting that Talaromyces spp. could live and survive better in the paddy soil. Five isolates, namely TF‐04, TF‐03, TF‐02, TF‐01, and TA‐02, were isolated from the paddy soil using sclerotia of R. solani as baits and were further evaluated for their activity against rice sheath blight. These isolates efficiently parasitized the hyphae and rotted the sclerotia even at higher water contents in sterilized sand and the soil. Isolate TF‐04 significantly promoted rice growth, reduced the severity of rice sheath blight, and increased rice yield under outdoor conditions. Defense related genes were upregulated and enzyme activities were enhanced in rice treated with isolate TF‐04. Our research supplies a microbiome guided approach to screen biological control agents, provides Talaromyces isolates to biologically control rice sheath blight.

ACS Style

Aqleem Abbas; Yanping Fu; Zheng Qu; Huizhang Zhao; Yongjian Sun; Yang Lin; Jiatao Xie; Jiasen Cheng; Daohong Jiang. Isolation and evaluation of the biocontrol potential of Talaromyces spp. against rice sheath blight guided by soil microbiome. Environmental Microbiology 2021, 1 .

AMA Style

Aqleem Abbas, Yanping Fu, Zheng Qu, Huizhang Zhao, Yongjian Sun, Yang Lin, Jiatao Xie, Jiasen Cheng, Daohong Jiang. Isolation and evaluation of the biocontrol potential of Talaromyces spp. against rice sheath blight guided by soil microbiome. Environmental Microbiology. 2021; ():1.

Chicago/Turabian Style

Aqleem Abbas; Yanping Fu; Zheng Qu; Huizhang Zhao; Yongjian Sun; Yang Lin; Jiatao Xie; Jiasen Cheng; Daohong Jiang. 2021. "Isolation and evaluation of the biocontrol potential of Talaromyces spp. against rice sheath blight guided by soil microbiome." Environmental Microbiology , no. : 1.

Brief report
Published: 21 January 2021 in Plants
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Considering the huge economic loss caused by postharvest diseases, the identification and prevention of citrus postharvest diseases is vital to the citrus industry. In 2018, 16 decayed citrus fruit from four citrus varieties—Satsuma mandarin (Citrus unshiu), Ponkan (Citrus reticulata Blanco cv. Ponkan), Nanfeng mandarin (Citrus reticulata cv. nanfengmiju), and Sugar orange (Citrus reticulata Blanco)—showing soft rot and sogginess on their surfaces and covered with white mycelia were collected from storage rooms in seven provinces. The pathogens were isolated and the pathogenicity of the isolates was tested. The fungal strains were identified as Lasiodiplodia pseudotheobromae based on their morphological characteristics and phylogenetic analyses using the internal transcribed spacer regions (ITS), translation elongation factor 1-α gene (TEF), and beta-tubulin (TUB) gene sequences. The strains could infect wounded citrus fruit and cause decay within two days post inoculation, but could not infect unwounded fruit. To our knowledge, this is the first report of citrus fruit decay caused by L. pseudotheobromae in China.

ACS Style

Jianghua Chen; Zihang Zhu; Yanping Fu; Jiasen Cheng; Jiatao Xie; Yang Lin. Identification of Lasiodiplodia pseudotheobromae Causing Fruit Rot of Citrus in China. Plants 2021, 10, 202 .

AMA Style

Jianghua Chen, Zihang Zhu, Yanping Fu, Jiasen Cheng, Jiatao Xie, Yang Lin. Identification of Lasiodiplodia pseudotheobromae Causing Fruit Rot of Citrus in China. Plants. 2021; 10 (2):202.

Chicago/Turabian Style

Jianghua Chen; Zihang Zhu; Yanping Fu; Jiasen Cheng; Jiatao Xie; Yang Lin. 2021. "Identification of Lasiodiplodia pseudotheobromae Causing Fruit Rot of Citrus in China." Plants 10, no. 2: 202.

Research article
Published: 12 January 2021 in Environmental Microbiology
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Fusarium graminearum is an important worldwide pathogen that causes Fusarium head blight (FHB) in wheat, barley, maize, and other grains. LncRNAs play important roles in many biological processes, but little is known about their functions and mechanisms in filamentous fungi. Here, we report that a natural antisense RNA, GzmetE‐AS, is transcribed from the opposite strand of GzmetE. GzmetE encodes a homoserine O‐acetyltransferase, which is important for sexual development and plant infection. The expression of GzmetE‐AS was increased significantly during the conidiation stage, while GzmetE was upregulated in the late stage of sexual reproduction. Overexpression of GzmetE‐AS inhibited the transcription of GzmetE. In contrast, the expression of GzmetE was significantly increased in GzmetE‐AS transcription termination strain GzmetE‐AS‐T. Furthermore, GzmetE‐AS‐T produced more perithecia and facilitated the ascospore discharge, resembling the phenotype of GzmetE overexpressing strains. However, overexpression of GzmetE‐AS in ∆dcl1/2 strain cannot inhibit the expression of GzmetE, and the GzmetE nat‐siRNA is also significantly reduced in ∆dcl1/2 mutant. Taken together, we have identified a novel antisense lncRNA GzmetE‐AS, which is involved in asexual and sexual reproduction by regulating its antisense gene GzmetE through RNAi pathway. Our findings reveal that the lncRNA plays critical roles in the development in F. graminearum.

ACS Style

Jie Wang; Wenping Zeng; Jiatao Xie; Yanping Fu; Daohong Jiang; Yang Lin; Weidong Chen; Jiasen Cheng. A novel antisense long non‐coding RNA participates in asexual and sexual reproduction by regulating the expression of GzmetE in Fusarium graminearum. Environmental Microbiology 2021, 1 .

AMA Style

Jie Wang, Wenping Zeng, Jiatao Xie, Yanping Fu, Daohong Jiang, Yang Lin, Weidong Chen, Jiasen Cheng. A novel antisense long non‐coding RNA participates in asexual and sexual reproduction by regulating the expression of GzmetE in Fusarium graminearum. Environmental Microbiology. 2021; ():1.

Chicago/Turabian Style

Jie Wang; Wenping Zeng; Jiatao Xie; Yanping Fu; Daohong Jiang; Yang Lin; Weidong Chen; Jiasen Cheng. 2021. "A novel antisense long non‐coding RNA participates in asexual and sexual reproduction by regulating the expression of GzmetE in Fusarium graminearum." Environmental Microbiology , no. : 1.

Journal article
Published: 03 December 2020 in Viruses
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Via virome sequencing, six viruses were detected from Magnaporthe oryzae strains YC81-2, including one virus in the family Tombusviridae, one virus in the family Narnaviridae and four viruses in the family Botourmiaviridae. Since the RNA-dependent RNA polymerase (RdRp) of one botourmiavirus show the highest identity (79%) with Magnaporthe oryzae ourmia-like virus 1 (MOLV1), the virus that was grouped into the genus Magoulivirus was designated as Magnaporthe oryzae botourmiavirus 2 (MOBV2). The three other novel botourmiaviruses were selected for further study. The complete nucleotide sequences of the three botourmiaviruses were determined. Sequence analysis showed that virus 1, virus 2, and virus 3 were 2598, 2385, and 2326 nts in length, respectively. The variable 3′ untranslated region (3′-UTR) and 5′-UTR of each virus could be folded into a stable stem-loop secondary structure. Each virus consisted of a unique ORF encoding a putative RdRp. The putative proteins with a conserved GDD motif of RdRp showed the highest sequence similarity to RdRps of viruses in the family Botourmiaviridae. Phylogenetic analysis demonstrated that these viruses were three distinct novel botourmiaviruses, clustered into the Botourmiaviridae family but not belonging to any known genera of this family. Thus, virus 1, virus 2, and virus 3 were designated as Magnaporthe oryzae botourmiavirus 5, 6, and 7 (MOBV5, MOBV6, and MOBV7), respectively. Our results suggest that four distinct botourmiaviruses, MOBV2, MOBV5, MOBV6, and MOBV7, co-infect a single strain of Magnaporthe oryzae, and MOBV5, MOBV6, and MOBV7 are members of three unclassified genera in the family Botourmiaviridae.

ACS Style

Yang Liu; Liyan Zhang; Ahmed Esmael; Jie Duan; Xuefeng Bian; Jichun Jia; Jiatao Xie; Jiasen Cheng; Yanping Fu; Daohong Jiang; Yang Lin. Four Novel Botourmiaviruses Co-Infecting an Isolate of the Rice Blast Fungus Magnaporthe oryzae. Viruses 2020, 12, 1383 .

AMA Style

Yang Liu, Liyan Zhang, Ahmed Esmael, Jie Duan, Xuefeng Bian, Jichun Jia, Jiatao Xie, Jiasen Cheng, Yanping Fu, Daohong Jiang, Yang Lin. Four Novel Botourmiaviruses Co-Infecting an Isolate of the Rice Blast Fungus Magnaporthe oryzae. Viruses. 2020; 12 (12):1383.

Chicago/Turabian Style

Yang Liu; Liyan Zhang; Ahmed Esmael; Jie Duan; Xuefeng Bian; Jichun Jia; Jiatao Xie; Jiasen Cheng; Yanping Fu; Daohong Jiang; Yang Lin. 2020. "Four Novel Botourmiaviruses Co-Infecting an Isolate of the Rice Blast Fungus Magnaporthe oryzae." Viruses 12, no. 12: 1383.

Journal article
Published: 08 June 2020 in Phytopathology Research
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Sclerotial development is a vital stage in the life cycles of many fungal plant pathogens. In this study, the protein Ss-CAD, which contains three conserved domains of cinnamyl alcohol dehydrogenase (CAD), was found to be required for sclerotial development in Sclerotinia sclerotiorum. Ss-CAD was significantly upregulated during early stage of sclerotial development. In Ss-CAD-silenced strains, sclerotial development was abnormal. In these silenced strains, formation of sclerotia was delayed or sclerotia yield was reduced, whereas hyphal growth and virulence were normal. Nox1, Nox2, and NoxR, which encode reactive oxygen species (ROS)-generating NADPH oxidases, were downregulated in Ss-CAD-silenced strains. NoxR-silenced strains displayed similar defects during sclerotial formation as Ss-CAD-silenced strains. Treatment of Ss-CAD-silenced strains with exogenous oxidants or NADPH restored normal sclerotial development. Sclerogenesis in Ss-CAD-silenced strains could also be recovered through Nox1 overexpression. The results suggest that Ss-CAD is linked to the NADPH oxidase pathways to affect sclerotial development in S. sclerotiorum.

ACS Style

Jiahong Zhou; Yang Lin; Yanping Fu; Jiatao Xie; Daohong Jiang; Jiasen Cheng. A cinnamyl alcohol dehydrogenase required for sclerotial development in Sclerotinia sclerotiorum. Phytopathology Research 2020, 2, 1 -11.

AMA Style

Jiahong Zhou, Yang Lin, Yanping Fu, Jiatao Xie, Daohong Jiang, Jiasen Cheng. A cinnamyl alcohol dehydrogenase required for sclerotial development in Sclerotinia sclerotiorum. Phytopathology Research. 2020; 2 (1):1-11.

Chicago/Turabian Style

Jiahong Zhou; Yang Lin; Yanping Fu; Jiatao Xie; Daohong Jiang; Jiasen Cheng. 2020. "A cinnamyl alcohol dehydrogenase required for sclerotial development in Sclerotinia sclerotiorum." Phytopathology Research 2, no. 1: 1-11.

Journal article
Published: 17 April 2020 in Viruses
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We previously identified Sclerotinia sclerotiorum negative-stranded virus 1 (SsNSRV-1), the first (−) ssRNA mycovirus, associated with hypovirulence of its fungal host Sclerotinia sclerotiorum. In this study, functional analysis of Open Reading Frame Ι (ORF Ι) of SsNSRV-1 was performed. The integration and expression of ORF Ι led to defects in hyphal tips, vegetative growth, and virulence of the mutant strains of S. sclerotiorum. Further, differentially expressed genes (DEGs) responding to the expression of ORF Ι were identified by transcriptome analysis. In all, 686 DEGs consisted of 267 up-regulated genes and 419 down-regulated genes. DEGs reprogramed by ORF Ι were relevant to secretory proteins, pathogenicity, transcription, transmembrane transport, protein biosynthesis, modification, and metabolism. Alternative splicing was also detected in all mutant strains, but not in hypovirulent strain AH98, which was co-infected by SsNSRV-1 and Sclerotinia sclerotiorum hypovirus 1 (SsHV-1). Thus, the integrity of SsNSRV-1 genome may be necessary to protect viral mRNA from splicing and inactivation by the host. Taken together, the results suggested that protein ORF Ι could regulate the transcription, translation, and modification of host genes in order to facilitate viral proliferation and reduce the virulence of the host. Therefore, ORF Ι may be a potential gene used for the prevention of S. sclerotiorum.

ACS Style

Zhixiao Gao; Junyan Wu; Daohong Jiang; Jiatao Xie; Jiasen Cheng; Yang Lin. ORF Ι of Mycovirus SsNSRV-1 is Associated with Debilitating Symptoms of Sclerotinia sclerotiorum. Viruses 2020, 12, 456 .

AMA Style

Zhixiao Gao, Junyan Wu, Daohong Jiang, Jiatao Xie, Jiasen Cheng, Yang Lin. ORF Ι of Mycovirus SsNSRV-1 is Associated with Debilitating Symptoms of Sclerotinia sclerotiorum. Viruses. 2020; 12 (4):456.

Chicago/Turabian Style

Zhixiao Gao; Junyan Wu; Daohong Jiang; Jiatao Xie; Jiasen Cheng; Yang Lin. 2020. "ORF Ι of Mycovirus SsNSRV-1 is Associated with Debilitating Symptoms of Sclerotinia sclerotiorum." Viruses 12, no. 4: 456.

Original article
Published: 27 February 2020 in Molecular Plant Pathology
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SsITL, a secretory protein of the necrotrophic phytopathogen Sclerotinia sclerotiorum, was previously reported to suppress host immunity at the early stages of infection. However, the molecular mechanism that SsITL uses to inhibit plant defence against S. sclerotiorum has not yet been elucidated. Here, we report that SsITL interacted with a chloroplast‐localized calcium‐sensing receptor, CAS, in chloroplasts. We found that CAS is a positive regulator of the salicylic acid signalling pathway in plant immunity to S. sclerotiorum and CAS‐mediated resistance against S. sclerotiorum depends on Ca2+ signalling. Furthermore, we showed that SsITL could interfere with the plant salicylic acid (SA) signalling pathway and SsITL‐expressing transgenic plants were more susceptible to S. sclerotiorum. However, truncated SsITLs (SsITL‐NT1 or SsITL‐CT1) that lost the ability to interact with CAS do not affect plant resistance to S. sclerotiorum. Taken together, our findings reveal that SsITL inhibits SA accumulation during the early stage of infection by interacting with CAS and then facilitating the infection by S. sclerotiorum.

ACS Style

Liguang Tang; Guogen Yang; Ming Ma; Xiaofan Liu; Bo Li; Jiatao Xie; Yanping Fu; Tao Chen; Yang Yu; Weidong Chen; Daohong Jiang; Jiasen Cheng. An effector of a necrotrophic fungal pathogen targets the calcium‐sensing receptor in chloroplasts to inhibit host resistance. Molecular Plant Pathology 2020, 21, 686 -701.

AMA Style

Liguang Tang, Guogen Yang, Ming Ma, Xiaofan Liu, Bo Li, Jiatao Xie, Yanping Fu, Tao Chen, Yang Yu, Weidong Chen, Daohong Jiang, Jiasen Cheng. An effector of a necrotrophic fungal pathogen targets the calcium‐sensing receptor in chloroplasts to inhibit host resistance. Molecular Plant Pathology. 2020; 21 (5):686-701.

Chicago/Turabian Style

Liguang Tang; Guogen Yang; Ming Ma; Xiaofan Liu; Bo Li; Jiatao Xie; Yanping Fu; Tao Chen; Yang Yu; Weidong Chen; Daohong Jiang; Jiasen Cheng. 2020. "An effector of a necrotrophic fungal pathogen targets the calcium‐sensing receptor in chloroplasts to inhibit host resistance." Molecular Plant Pathology 21, no. 5: 686-701.

Journal article
Published: 17 January 2020 in International Journal of Molecular Sciences
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Botrytis cinerea, a ubiquitous necrotrophic plant-pathogenic fungus, is responsible for grey mold and rot disease in a very wide range of plant species. Subtilisin-like proteases (or subtilases) are a very diverse family of serine proteases present in many organisms and are reported to have a broad spectrum of biological functions. Here, we identified two genes encoding subtilisin-like proteases (Bcser1 and Bcser2) in the genome of B. cinerea, both of which contain an inhibitor I9 domain and a peptidase S8 domain. The expression levels of Bcser1 and Bcser2 increased during the sclerotial forming stage, as well as during a later stage of hyphal infection on Arabidopsis thaliana leaves, but the up-regulation of Bcser1 was significantly higher than that of Bcser2. Interestingly, deletion of Bcser1 had no effect on the fungal development or virulence of B. cinerea. However, deletion of Bcser2 or double deletion of Bcser1 and Bcser2 severely impaired the hyphal growth, sclerotial formation and conidiation of B. cinerea. We also found that ∆Bcser2 and ∆Bcser1/2 could not form complete infection cushions and then lost the ability to infect intact plant leaves of Arabidopsis and tomato but could infect wounded plant tissues. Taken together, our results indicate that the subtilisin-like protease Bcser2 is crucial for the sclerotial formation, conidiation, and virulence of B. cinerea.

ACS Style

Xinqiang Liu; Jiatao Xie; Yanping Fu; Daohong Jiang; Tao Chen; Jiasen Cheng. The Subtilisin-Like Protease Bcser2 Affects the Sclerotial Formation, Conidiation and Virulence of Botrytis cinerea. International Journal of Molecular Sciences 2020, 21, 603 .

AMA Style

Xinqiang Liu, Jiatao Xie, Yanping Fu, Daohong Jiang, Tao Chen, Jiasen Cheng. The Subtilisin-Like Protease Bcser2 Affects the Sclerotial Formation, Conidiation and Virulence of Botrytis cinerea. International Journal of Molecular Sciences. 2020; 21 (2):603.

Chicago/Turabian Style

Xinqiang Liu; Jiatao Xie; Yanping Fu; Daohong Jiang; Tao Chen; Jiasen Cheng. 2020. "The Subtilisin-Like Protease Bcser2 Affects the Sclerotial Formation, Conidiation and Virulence of Botrytis cinerea." International Journal of Molecular Sciences 21, no. 2: 603.

Journal article
Published: 16 August 2019 in Viruses
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Infection by diverse mycoviruses is a common phenomenon in Sclerotinia sclerotiorum. In this study, the full genome of a single-stranded RNA mycovirus, tentatively named Hubei sclerotinia RNA virus 1 (HuSRV1), was determined in the hypovirulent strain 277 of S. sclerotiorum. The HuSRV1 genome is 4492 nucleotides (nt) long and lacks a poly (A) tail at the 3'- terminus. Sequence analyses showed that the HuSRV1 genome contains four putative open reading frames (ORFs). ORF1a was presumed to encode a protein with a conserved protease domain and a transmembrane domain. This protein is 27% identical to the P2a protein encoded by the subterranean clover mottle virus. ORF1b encodes a protein containing a conserved RNA-dependent RNA polymerase (RdRp) domain, which may be translated into a fusion protein by a -1 ribosome frameshift. This protein is 45.9% identical to P2b encoded by the sowbane mosaic virus. ORF2 was found to encode a putative coat protein, which shares 23% identical to the coat protein encoded by the olive mild mosaic virus. ORF3 was presumed to encode a putative protein with an unknown function. Evolutionary relation analyses indicated that HuSRV1 is related to members within Sobemovirus, but forms a unique phylogenetic branch, suggesting that HuSRV1 represents a new member within Solemoviridae. HuSRV1 virions, approximately 30 nm in diameter, were purified from strain 277. The purified virions were successfully introduced into virulent strain Ep-1PNA367, resulting in a new hypovirulent strain, which confirmed that HuSRV1 confers hypovirulence on S. sclerotiorum.

ACS Style

Ayesha Azhar; Fan Mu; Huang Huang; Jiasen Cheng; Yanping Fu; Muhammad Rizwan Hamid; Daohong Jiang; Jiatao Xie; Mu; Fu; Xie. A Novel RNA Virus Related to Sobemoviruses Confers Hypovirulence on the Phytopathogenic Fungus Sclerotinia sclerotiorum. Viruses 2019, 11, 759 .

AMA Style

Ayesha Azhar, Fan Mu, Huang Huang, Jiasen Cheng, Yanping Fu, Muhammad Rizwan Hamid, Daohong Jiang, Jiatao Xie, Mu, Fu, Xie. A Novel RNA Virus Related to Sobemoviruses Confers Hypovirulence on the Phytopathogenic Fungus Sclerotinia sclerotiorum. Viruses. 2019; 11 (8):759.

Chicago/Turabian Style

Ayesha Azhar; Fan Mu; Huang Huang; Jiasen Cheng; Yanping Fu; Muhammad Rizwan Hamid; Daohong Jiang; Jiatao Xie; Mu; Fu; Xie. 2019. "A Novel RNA Virus Related to Sobemoviruses Confers Hypovirulence on the Phytopathogenic Fungus Sclerotinia sclerotiorum." Viruses 11, no. 8: 759.

Original article
Published: 09 May 2019 in Molecular Plant Pathology
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Sclerotinia sclerotiorum is a devastating necrotrophic fungal pathogen that infects over 400 species of plants worldwide. Reactive oxygen species (ROS) modulations are critical for the pathogenic development of S. sclerotiorum. The fungus applies enzymatic and non‐enzymatic antioxidants to cope with the oxidative stress during the infection processes. Survival factor 1 was identified and characterized to promote survival under conditions of oxidative stress in Saccharomyes cerevisiae. In this research, a gene named SsSvf1 was predicted to encode a survival factor 1 homologue in S. sclerotiorum. SsSvf1 transcripts showed high expression levels in hyphae under oxidative stress. Silencing of SsSvf1 resulted in increased sensitivity to oxidative stress in culture and increased levels of intracellular ROS. Transcripts of SsSvf1 showed a dramatic increase during the initial stage of infection and the gene‐silenced strains displayed reduced virulence on oilseed rape and Arabidopsis thaliana. Inhibition of plant ROS production partially restores virulence of SsSvf1 gene‐silenced strains. SsSvf1 gene‐silenced strains exhibited normal oxalate production, but were impaired in compound appressorium formation and cell wall integrity. The results suggest that SsSvf1 is involved in coping with ROS during fungal‐host interactions and plays a crucial role in the pathogenicity of S. sclerotiorum.

ACS Style

Yang Yu; Jiao Du; Yabo Wang; Mengyao Zhang; Zhiqiang Huang; Junsong Cai; Anfei Fang; Yuheng Yang; Ling Qing; Chaowei Bi; Jiasen Cheng. Survival factor 1 contributes to the oxidative stress response and is required for full virulence ofSclerotinia sclerotiorum. Molecular Plant Pathology 2019, 20, 895 -906.

AMA Style

Yang Yu, Jiao Du, Yabo Wang, Mengyao Zhang, Zhiqiang Huang, Junsong Cai, Anfei Fang, Yuheng Yang, Ling Qing, Chaowei Bi, Jiasen Cheng. Survival factor 1 contributes to the oxidative stress response and is required for full virulence ofSclerotinia sclerotiorum. Molecular Plant Pathology. 2019; 20 (7):895-906.

Chicago/Turabian Style

Yang Yu; Jiao Du; Yabo Wang; Mengyao Zhang; Zhiqiang Huang; Junsong Cai; Anfei Fang; Yuheng Yang; Ling Qing; Chaowei Bi; Jiasen Cheng. 2019. "Survival factor 1 contributes to the oxidative stress response and is required for full virulence ofSclerotinia sclerotiorum." Molecular Plant Pathology 20, no. 7: 895-906.

Journal article
Published: 19 March 2019 in Viruses
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We previously determined that virions of Sclerotinia sclerotiorum hypovirulence associated DNA virus 1 (SsHADV-1) could directly infect hyphae of Sclerotinia sclerotiorum, resulting in hypovirulence of the fungal host. However, the molecular mechanisms of SsHADV-1 virions disruption of the fungal cell wall barrier and entrance into the host cell are still unclear. To investigate the early response of S. sclerotiorum to SsHADV-1 infection, S. sclerotiorum hyphae were inoculated with purified SsHADV-1 virions. The pre- and post-infection hyphae were collected at one⁻three hours post-inoculation for transcriptome analysis. Further, bioinformatic analysis showed that differentially expressed genes (DEGs) regulated by SsHADV-1 infection were identified in S. sclerotiorum. In total, 187 genes were differentially expressed, consisting of more up-regulated (114) than down-regulated (73) genes. The identified DEGs were involved in several important pathways. Metabolic processes, biosynthesis of antibiotics, and secondary metabolites were the most affected categories in S. sclerotiorum upon SsHADV-1 infection. Cell structure analysis suggested that 26% of the total DEGs were related to membrane tissues. Furthermore, 10 and 27 DEGs were predicted to be located in the cell membrane and mitochondria, respectively. Gene ontology enrichment analyses of the DEGs were performed, followed by functional annotation of the genes. Interestingly, one third of the annotated functional DEGs could be involved in the Ras-small G protein signal transduction pathway. These results revealed that SsHADV-1 virions may be able to bind host membrane proteins and influence signal transduction through Ras-small G protein-coupled receptors during early infection, providing new insight towards the molecular mechanisms of virions infection in S. sclerotiorum.

ACS Style

Feng Ding; Jiasen Cheng; Yanping Fu; Tao Chen; Bo Li; Daohong Jiang; Jiatao Xie. Early Transcriptional Response to DNA Virus Infection in Sclerotinia sclerotiorum. Viruses 2019, 11, 278 .

AMA Style

Feng Ding, Jiasen Cheng, Yanping Fu, Tao Chen, Bo Li, Daohong Jiang, Jiatao Xie. Early Transcriptional Response to DNA Virus Infection in Sclerotinia sclerotiorum. Viruses. 2019; 11 (3):278.

Chicago/Turabian Style

Feng Ding; Jiasen Cheng; Yanping Fu; Tao Chen; Bo Li; Daohong Jiang; Jiatao Xie. 2019. "Early Transcriptional Response to DNA Virus Infection in Sclerotinia sclerotiorum." Viruses 11, no. 3: 278.

Research article
Published: 01 June 2018 in Phytopathology®
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Mitogen-activated protein kinase (MAPK) cascades play a central role in cellular growth, proliferation, and survival. MAPK cascade genes have been extensively investigated in model plants, mammals, yeast, and fungi but are not characterized in Plasmodiophora brassicae, which causes clubroot disease in cruciferous plants. Here, we identified 7 PbMAPK, 3 PbMAPKK, and 9 PbMAPKKK genes in the P. brassicae genome. Transcriptional profiling analysis demonstrated that several MAPK, MAPK kinase (MAPKK), and MAPK kinase kinase (MAPKKK) genes were preferentially expressed in three different zoosporic stages. Based on yeast two-hybrid assays, PbMAKKK7 interacted with PbMAKK3 and PbMAKK3 interacted with PbMAK1/PbMAK3. The PbMAKKK7-PbMAKK3-PbMAK1/PbMAK3 cascade may be present in P. brassicae. U0126, a potent and specific inhibitor of MAPKK, could inhibit the germination of P. brassicae resting spores. U0126 was used to treat the resting spores of P. brassicae and coinoculate rapeseed, and was proven to significantly relieve the severity of clubroot symptoms in the host plant and delay the life cycle of P. brassicae. These results suggest that MAPK signaling pathways may play important roles in P. brassicae growth, development, and pathogenicity.

ACS Style

Tao Chen; Kai Bi; Yanli Zhao; Xueliang Lyu; Zhixiao Gao; Ying Zhao; Yanping Fu; Jiasen Cheng; Jiatao Xie; Daohong Jiang. MAPKK Inhibitor U0126 Inhibits Plasmodiophora brassicae Development. Phytopathology® 2018, 108, 711 -720.

AMA Style

Tao Chen, Kai Bi, Yanli Zhao, Xueliang Lyu, Zhixiao Gao, Ying Zhao, Yanping Fu, Jiasen Cheng, Jiatao Xie, Daohong Jiang. MAPKK Inhibitor U0126 Inhibits Plasmodiophora brassicae Development. Phytopathology®. 2018; 108 (6):711-720.

Chicago/Turabian Style

Tao Chen; Kai Bi; Yanli Zhao; Xueliang Lyu; Zhixiao Gao; Ying Zhao; Yanping Fu; Jiasen Cheng; Jiatao Xie; Daohong Jiang. 2018. "MAPKK Inhibitor U0126 Inhibits Plasmodiophora brassicae Development." Phytopathology® 108, no. 6: 711-720.

Journal article
Published: 31 May 2018 in Viruses
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Various mycoviruses have been isolated from Sclerotinia sclerotiorum. Here, we identified a viral RNA sequence contig, representing a novel virus, Sclerotinia sclerotiorum deltaflexivirus 2 (SsDFV2), from an RNA_Seq database. We found that SsDFV2 was harbored in the hypovirulent strain, 228, which grew slowly on potato dextrose agar, produced a few sclerotia, and could not induce typical lesions on detached rapeseed (Brassica napus) leaves. Strain 228 was also infected by Botrytis porri RNA Virus 1 (BpRV1), a virus originally isolated from Botrytis porri. The genome of SsDFV2 comprised 6711 nucleotides, excluding the poly (A) tail, and contained a single large predicted open reading frame encoding a putative viral RNA replicase. Phylogenetic analysis demonstrated that SsDFV2 is closely related to viruses in the family Deltaflexiviridae; however, it also differs significantly from members of this family, suggesting that it may represent a new species. Further we determined that SsDFV2 could be efficiently transmitted to host vegetative incompatible individuals by dual culture. To our best knowledge, this is the first report that a (+) ssRNA mycovirus can overcome the transmission limitations of the vegetative incompatibility system, a phenomenon that may facilitate the potential use of mycoviruses for the control of crop fungal diseases.

ACS Style

Muhammad Rizwan Hamid; Jiatao Xie; Songsong Wu; Shahzeen Kanwal Maria; Dan Zheng; Abdoulaye Assane Hamidou; Qihua Wang; Jiasen Cheng; Yanping Fu; Daohong Jiang. A Novel Deltaflexivirus that Infects the Plant Fungal Pathogen, Sclerotinia sclerotiorum, Can Be Transmitted Among Host Vegetative Incompatible Strains. Viruses 2018, 10, 295 .

AMA Style

Muhammad Rizwan Hamid, Jiatao Xie, Songsong Wu, Shahzeen Kanwal Maria, Dan Zheng, Abdoulaye Assane Hamidou, Qihua Wang, Jiasen Cheng, Yanping Fu, Daohong Jiang. A Novel Deltaflexivirus that Infects the Plant Fungal Pathogen, Sclerotinia sclerotiorum, Can Be Transmitted Among Host Vegetative Incompatible Strains. Viruses. 2018; 10 (6):295.

Chicago/Turabian Style

Muhammad Rizwan Hamid; Jiatao Xie; Songsong Wu; Shahzeen Kanwal Maria; Dan Zheng; Abdoulaye Assane Hamidou; Qihua Wang; Jiasen Cheng; Yanping Fu; Daohong Jiang. 2018. "A Novel Deltaflexivirus that Infects the Plant Fungal Pathogen, Sclerotinia sclerotiorum, Can Be Transmitted Among Host Vegetative Incompatible Strains." Viruses 10, no. 6: 295.

Journal article
Published: 26 April 2018 in Frontiers in Microbiology
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Ascospores act as the primary inoculum of Fusarium graminearum, which causes the destructive disease Fusarium head blight (FHB), or scab. MicroRNAs (miRNAs) have been reported in the F. graminearum vegetative stage, and Fgdcl2 is involved in microRNA-like RNA (milRNA) biogenesis but has no major impact on vegetative growth, abiotic stress or pathogenesis. In the present study, we found that ascospore discharge was decreased in the Fgdcl1 deletion mutant, and completely blocked in the double-deletion mutant of Fgdcl1 and Fgdcl2. Besides, more immature asci were observed in the double-deletion mutant. Interestingly, the up-regulated differentially expressed genes (DEGs) common to ΔFgdcl1 and ΔFgdcl1/2 were related to ion transmembrane transporter and membrane components. The combination of small RNA and transcriptome sequencing with bioinformatics analysis predicted 143 novel milRNAs in wild-type perithecia, and 138 of these milRNAs partly or absolutely depended on Fgdcl1, while only 5 novel milRNAs were still obtained in the Fgdcl1 and Fgdcl2 double-deletion mutant. Furthermore, 117 potential target genes were predicted. Overall, Fgdcl1 and Fgdcl2 genes were partly functionally redundant in ascospore discharge and perithecium-specific milRNA generation in F. graminearum, and these perithecium-specific milRNAs play potential roles in sexual development.

ACS Style

Wenping Zeng; Jie Wang; Ying Wang; Jing Lin; Yanping Fu; Jiatao Xie; Daohong Jiang; Tao Chen; Huiquan Liu; Jiasen Cheng. Dicer-Like Proteins Regulate Sexual Development via the Biogenesis of Perithecium-Specific MicroRNAs in a Plant Pathogenic Fungus Fusarium graminearum. Frontiers in Microbiology 2018, 9, 818 .

AMA Style

Wenping Zeng, Jie Wang, Ying Wang, Jing Lin, Yanping Fu, Jiatao Xie, Daohong Jiang, Tao Chen, Huiquan Liu, Jiasen Cheng. Dicer-Like Proteins Regulate Sexual Development via the Biogenesis of Perithecium-Specific MicroRNAs in a Plant Pathogenic Fungus Fusarium graminearum. Frontiers in Microbiology. 2018; 9 ():818.

Chicago/Turabian Style

Wenping Zeng; Jie Wang; Ying Wang; Jing Lin; Yanping Fu; Jiatao Xie; Daohong Jiang; Tao Chen; Huiquan Liu; Jiasen Cheng. 2018. "Dicer-Like Proteins Regulate Sexual Development via the Biogenesis of Perithecium-Specific MicroRNAs in a Plant Pathogenic Fungus Fusarium graminearum." Frontiers in Microbiology 9, no. : 818.

Brief report
Published: 03 November 2017 in Archives of Virology
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Seven dsRNA segments were detected from a single Rhizoctonia solani strain HG81. From the full-length cDNA sequences of four smaller dsRNA segments, the genomes of two related partitiviruses, designated as Rhizoctonia solani partitivirus 3 (RsPV3) and RsPV4, were determined. The genomes of RsPV3 and RsPV4 are both composed of two separate dsRNA segments, with each segment possessing a single open reading frame (ORF). ORF1 from RsPV3 and RsPV4 encodes a putative RNA-dependent RNA polymerase, while ORF2 of RsPV3 and RsPV4 encodes a putative capsid protein. RsPV3 and RsPV4 share high sequence identity with viruses classified within the genus Alphapartitivirus, family Partitiviridae.

ACS Style

Ruiling Lyu; Yi Zhang; Qing Tang; Lyu Ruiling; Jiasen Cheng; Yanping Fu; Tao Chen; Daohong Jiang; Jiatao Xie. Two alphapartitiviruses co-infecting a single isolate of the plant pathogenic fungus Rhizoctonia solani. Archives of Virology 2017, 163, 515 -520.

AMA Style

Ruiling Lyu, Yi Zhang, Qing Tang, Lyu Ruiling, Jiasen Cheng, Yanping Fu, Tao Chen, Daohong Jiang, Jiatao Xie. Two alphapartitiviruses co-infecting a single isolate of the plant pathogenic fungus Rhizoctonia solani. Archives of Virology. 2017; 163 (2):515-520.

Chicago/Turabian Style

Ruiling Lyu; Yi Zhang; Qing Tang; Lyu Ruiling; Jiasen Cheng; Yanping Fu; Tao Chen; Daohong Jiang; Jiatao Xie. 2017. "Two alphapartitiviruses co-infecting a single isolate of the plant pathogenic fungus Rhizoctonia solani." Archives of Virology 163, no. 2: 515-520.

Journal article
Published: 27 October 2017 in New Phytologist
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Cerato-platanin proteins (CPs), which are secreted by filamentous fungi, are phytotoxic to host plants, but their functions have not been well defined to date. Here we characterized a CP (SsCP1) from the necrotrophic phytopathogen Sclerotinia sclerotiorum. Sscp1 transcripts accumulated during plant infection, and deletion of Sscp1 significantly reduced virulence. SsCP1 could induce significant cell death when expressed in Nicotiana benthamiana. Using yeast two-hybrid, GST pull-down, co-immunoprecipitation and bimolecular florescence complementation, we found that SsCP1 interacts with PR1 in the apoplast to facilitate infection by S. sclerotiorum. Overexpressing PR1 enhanced resistance to the wild-type strain, but not to the Sscp1 knockout strain of S. sclerotiorum. Sscp1-expressing transgenic plants showed increased concentrations of salicylic acid (SA) and higher levels of resistance to several plant pathogens (namely Botrytis cinerea, Alternaria brassicicola and Golovinomyces orontii). Our results suggest that SsCP1 is important for virulence of S. sclerotiorum and that it can be recognized by plants to trigger plant defense responses. Our results also suggest that the SA signaling pathway is involved in CP-mediated plant defense .

ACS Style

Guogen Yang; Liguang Tang; Yingdi Gong; Jiatao Xie; Yanping Fu; Daohong Jiang; Tang Liguang; David B. Collinge; Weidong Chen; Jiasen Cheng. A cerato-platanin protein SsCP1 targets plant PR1 and contributes to virulence ofSclerotinia sclerotiorum. New Phytologist 2017, 217, 739 -755.

AMA Style

Guogen Yang, Liguang Tang, Yingdi Gong, Jiatao Xie, Yanping Fu, Daohong Jiang, Tang Liguang, David B. Collinge, Weidong Chen, Jiasen Cheng. A cerato-platanin protein SsCP1 targets plant PR1 and contributes to virulence ofSclerotinia sclerotiorum. New Phytologist. 2017; 217 (2):739-755.

Chicago/Turabian Style

Guogen Yang; Liguang Tang; Yingdi Gong; Jiatao Xie; Yanping Fu; Daohong Jiang; Tang Liguang; David B. Collinge; Weidong Chen; Jiasen Cheng. 2017. "A cerato-platanin protein SsCP1 targets plant PR1 and contributes to virulence ofSclerotinia sclerotiorum." New Phytologist 217, no. 2: 739-755.

Annotated sequence record
Published: 25 January 2017 in Archives of Virology
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A double-stranded RNA (dsRNA) segment was isolated from the filamentous phytopathogenic fungus Rhizoctonia oryzae-sativae and its full-length cDNA sequence (3038 nucleotides) was determined. Sequence analysis revealed that a large open reading frame (ORF) is present on the positive strand of this dsRNA segment when the mitochondrial genetic code was applied. The ORF encodes a putative RNA-dependent RNA polymerase, which shares the closest similarity with Rhizoctonia mitovirus 1 and Rhizophagus sp. RF1 mitovirus, with 43% and 29% identity, respectively. This dsRNA segment represents the replication form of a novel mitovirus that was temporarily designated Rhizoctonia oryzae-sativae mitovirus 1 (RoMV1). Phylogenetic analysis further suggested that RoMV1 belongs to the family Narnaviridae. This is the first study to report a mitovirus genome sequence in the phytopathogenic fungus R. oryzae-sativae.

ACS Style

Assane Hamidou Abdoulaye; Jiasen Cheng; Yanping Fu; Daohong Jiang; Jiatao Xie. Complete genome sequence of a novel mitovirus from the phytopathogenic fungus Rhizoctonia oryzae-sativae. Archives of Virology 2017, 162, 1409 -1412.

AMA Style

Assane Hamidou Abdoulaye, Jiasen Cheng, Yanping Fu, Daohong Jiang, Jiatao Xie. Complete genome sequence of a novel mitovirus from the phytopathogenic fungus Rhizoctonia oryzae-sativae. Archives of Virology. 2017; 162 (5):1409-1412.

Chicago/Turabian Style

Assane Hamidou Abdoulaye; Jiasen Cheng; Yanping Fu; Daohong Jiang; Jiatao Xie. 2017. "Complete genome sequence of a novel mitovirus from the phytopathogenic fungus Rhizoctonia oryzae-sativae." Archives of Virology 162, no. 5: 1409-1412.

Research article
Published: 22 April 2016 in Environmental Microbiology
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Coniothyrium minitans is an important sclerotial and hyphal parasite of the plant pathogen Sclerotinia sclerotiorum. Previously, a conidiation‐deficient mutant, ZS‐1N22225, was screened from a T‐DNA insertional library of C. minitans. CmVps39, a homologue of Vam6p/Vps39p that plays a critical role in vacuolar morphogenesis in yeast, was disrupted by a T‐DNA insertion in this mutant. CmVps39 is composed of 1071 amino acids with an amino‐terminal citron homology domain and a central clathrin homology domain, as observed for other Vam6p/Vps39p family proteins. Abnormal fragmented vacuoles were observed in ΔCmVps39 under light microscopy and transmission electron microscopy, and ΔCmVps39 showed impairment in autophagy. ΔCmVps39 also exhibited significantly reduced hyphal development, poor conidiation and decreased sclerotial mycoparasitism. In addition, deletion of CmVps39 affected osmotic adaptation, pH homeostasis and cell wall integrity. Taken together, our results suggest that CmVps39 has an essential function in vacuolar morphology, autophagy, fungal development and mycoparasitism in C. minitans.

ACS Style

Xiaoxiang Yang; Hui Cui; Jiasen Cheng; Jiatao Xie; Daohong Jiang; Tom Hsiang; Yanping Fu. A HOPS protein, CmVps39, is required for vacuolar morphology, autophagy, growth, conidiogenesis and mycoparasitic functions ofConiothyrium minitans. Environmental Microbiology 2016, 18, 3785 -3797.

AMA Style

Xiaoxiang Yang, Hui Cui, Jiasen Cheng, Jiatao Xie, Daohong Jiang, Tom Hsiang, Yanping Fu. A HOPS protein, CmVps39, is required for vacuolar morphology, autophagy, growth, conidiogenesis and mycoparasitic functions ofConiothyrium minitans. Environmental Microbiology. 2016; 18 (11):3785-3797.

Chicago/Turabian Style

Xiaoxiang Yang; Hui Cui; Jiasen Cheng; Jiatao Xie; Daohong Jiang; Tom Hsiang; Yanping Fu. 2016. "A HOPS protein, CmVps39, is required for vacuolar morphology, autophagy, growth, conidiogenesis and mycoparasitic functions ofConiothyrium minitans." Environmental Microbiology 18, no. 11: 3785-3797.

Research article
Published: 01 February 2016 in PLOS Pathogens
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Small, secreted proteins have been found to play crucial roles in interactions between biotrophic/hemi-biotrophic pathogens and plants. However, little is known about the roles of these proteins produced by broad host-range necrotrophic phytopathogens during infection. Here, we report that a cysteine-rich, small protein SsSSVP1 in the necrotrophic phytopathogen Sclerotinia sclerotiorum was experimentally confirmed to be a secreted protein, and the secretion of SsSSVP1 from hyphae was followed by internalization and cell-to-cell movement independent of a pathogen in host cells. SsSSVP1∆SP could induce significant plant cell death and targeted silencing of SsSSVP1 resulted in a significant reduction in virulence. Through yeast two-hybrid (Y2H), coimmunoprecipitation (co-IP) and bimolecular fluorescence complementation (BiFC) assays, we demonstrated that SsSSVP1∆SP interacted with QCR8, a subunit of the cytochrome b-c1 complex of mitochondrial respiratory chain in plants. Double site-directed mutagenesis of two cysteine residues (C38 and C44) in SsSSVP1∆SP had significant effects on its homo-dimer formation, SsSSVP1∆SP-QCR8 interaction and plant cell death induction, indicating that partial cysteine residues surely play crucial roles in maintaining the structure and function of SsSSVP1. Co-localization and BiFC assays showed that SsSSVP1∆SP might hijack QCR8 to cytoplasm before QCR8 targeting into mitochondria, thereby disturbing its subcellular localization in plant cells. Furthermore, virus induced gene silencing (VIGS) of QCR8 in tobacco caused plant abnormal development and cell death, indicating the cell death induced by SsSSVP1∆SP might be caused by the SsSSVP1∆SP-QCR8 interaction, which had disturbed the QCR8 subcellular localization and hence disabled its biological functions. These results suggest that SsSSVP1 is a potential effector which may manipulate plant energy metabolism to facilitate the infection of S. sclerotiorum. Our findings indicate novel roles of small secreted proteins in the interactions between host-non-specific necrotrophic fungi and plants, and highlight the significance to illuminate the pathogenic mechanisms of this type of interaction. To resist biotrophic and hemibiotrophic phytopathogens, plants utilize an innate immune system, mediated through nucleotide binding (NB)-leucine rich repeat (LRR) proteins, to respond to effectors, most of which are small secreted proteins. Hypersensitive responses (HRs) resulting from this type of interaction can effectively restrain the expansion of biotrophic or hemibiotrophic phytopathogens in plant tissues. However, it is not effective against typical necrotrophs with remarkably broad host range, such as S. sclerotiorum, because these necrotrophs have long been thought to just simply kill hosts and complete their life cycles using nutrients derived mostly from dead plant tissues. This type of phytopathogen-plant interaction obviously does not comply with the gene-for-gene or inversed gene-for-gene relationship. The results in present study show that SsSSVP1 of S. sclerotiorum functions as an effector in pathogen-plant interactions. SsSSVP1 is dramatically induced during infection, and required for the full virulence of S. sclerotiorum. SsSSVP1 can be internalized by plant cells after being secreted from fungal cells in the absence of a pathogen during infection. Furthermore, SsSSVP1∆SP interacts with QCR8, a subunit of cytochrome b-c1 complex, and disturbs the localization of QCR8 in mitochondria, which may disable its biological function. The nonfunctionalization of QCR8 caused significant plant cell death. Hence, SsSSVP1 acts as an effector to manipulate the host cell physiology to facilitate the colonization of S. sclerotiorum. Obviously, this is a completely different interaction model from the gene-for-gene or inversed gene-for-gene paradigm. These findings suggest that the pathogenesis of S. sclerotiorum is more subtle and complex than previously appreciated and highlight the significance to investigate the interaction models between the host-non-specific necrotrophs and their hosts.

ACS Style

Xueliang Lyu; Cuicui Shen; Yanping Fu; Jiatao Xie; Daohong Jiang; Guoqing Li; Jiasen Cheng. A Small Secreted Virulence-Related Protein Is Essential for the Necrotrophic Interactions of Sclerotinia sclerotiorum with Its Host Plants. PLOS Pathogens 2016, 12, e1005435 .

AMA Style

Xueliang Lyu, Cuicui Shen, Yanping Fu, Jiatao Xie, Daohong Jiang, Guoqing Li, Jiasen Cheng. A Small Secreted Virulence-Related Protein Is Essential for the Necrotrophic Interactions of Sclerotinia sclerotiorum with Its Host Plants. PLOS Pathogens. 2016; 12 (2):e1005435.

Chicago/Turabian Style

Xueliang Lyu; Cuicui Shen; Yanping Fu; Jiatao Xie; Daohong Jiang; Guoqing Li; Jiasen Cheng. 2016. "A Small Secreted Virulence-Related Protein Is Essential for the Necrotrophic Interactions of Sclerotinia sclerotiorum with Its Host Plants." PLOS Pathogens 12, no. 2: e1005435.