This page has only limited features, please log in for full access.

Unclaimed
Serena Reggi
Department of Health, Animal Science and Food Safety “Carlo Cantoni” (VESPA), Università Degli Studi di Milano, 26900 Lodi, Italy

Honors and Awards

The user has no records in this section


Career Timeline

The user has no records in this section.


Short Biography

The user biography is not available.
Following
Followers
Co Authors
The list of users this user is following is empty.
Following: 0 users

Feed

Journal article
Published: 26 July 2021 in Microorganisms
Reads 0
Downloads 0

Limosilactobacillus reuteri and Lactiplantibacillus plantarum strains, previously isolated from weaned piglets, were considered for the evaluation of their adhesive characteristics. Lactobacilli were treated with LiCl in order to remove the surface protein layer, and probiotic activity was compared with those of untreated strains. The autoaggregation, co-aggregation to E. coli F18+, and adhesive abilities of LiCl-treated Limosilactobacillus reuteri and Lactiplantibacillus plantarum were significantly inhibited (p< 0.05) compared with the respective untreated strain. The hydrophobic and basic phenotypes were observed due to the strong affinity to chloroform and low adherence to ethyl acetate. In particular, L. plantarum showed higher hydrophobicity compared to L. reuteri, which may reflect their different colonizing ability. After treatment with LiCl to remove surface proteins, the adherence capabilities of L. reuteri and L. casei on IPEC-J2 cells decreased significantly (p< 0.001) and L. reuteri adhered more frequently. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) showed that both L. reuteri and L. plantarum had several bands ranging from 20 to 100 kDa. Two-dimensional gel electrophoresis showed an acidic profile of the surface-layer polypeptides for both bacterial strains, and more studies are needed to characterize their profile and functions. The results confirm the pivotal role of surface proteins in the probiotic potential of L. reuteri and L. plantarum.

ACS Style

Matteo Dell’Anno; Carlotta Giromini; Serena Reggi; Mariagrazia Cavalleri; Alessandra Moscatelli; Elisabetta Onelli; Raffaella Rebucci; Tamil Sundaram; Simona Coranelli; Ambra Spalletta; Antonella Baldi; Luciana Rossi. Evaluation of Adhesive Characteristics of L. plantarum and L. reuteri Isolated from Weaned Piglets. Microorganisms 2021, 9, 1587 .

AMA Style

Matteo Dell’Anno, Carlotta Giromini, Serena Reggi, Mariagrazia Cavalleri, Alessandra Moscatelli, Elisabetta Onelli, Raffaella Rebucci, Tamil Sundaram, Simona Coranelli, Ambra Spalletta, Antonella Baldi, Luciana Rossi. Evaluation of Adhesive Characteristics of L. plantarum and L. reuteri Isolated from Weaned Piglets. Microorganisms. 2021; 9 (8):1587.

Chicago/Turabian Style

Matteo Dell’Anno; Carlotta Giromini; Serena Reggi; Mariagrazia Cavalleri; Alessandra Moscatelli; Elisabetta Onelli; Raffaella Rebucci; Tamil Sundaram; Simona Coranelli; Ambra Spalletta; Antonella Baldi; Luciana Rossi. 2021. "Evaluation of Adhesive Characteristics of L. plantarum and L. reuteri Isolated from Weaned Piglets." Microorganisms 9, no. 8: 1587.

Journal article
Published: 12 June 2021 in Animals
Reads 0
Downloads 0

The effects of Lactobacillus plantarum and Lactobacillus reuteri and their combination were assessed in weaned piglets. Three hundred and fifty weaned piglets (Landrace × Large White), balanced in terms of weight and sex, were randomly allotted to four experimental groups (25 pens, 14 piglets/pen). Piglets were fed a basal control diet (CTRL, six pens) and a treatment diet supplemented with 2 × 108 CFU/g of L. plantarum (PLA, 6 pens), 2 × 108 CFU/g L. reuteri (REU, six pens) and the combination of both bacterial strains (1 × 108 CFU/g of L. plantarum combined with 1 × 108 CFU/g of L. reuteri, P+R, 7 pens) for 28 days. Body weight and feed intake were recorded weekly. Diarrhoea occurrence was assessed weekly by the faecal score (0–3; considering diarrhoea ≥ 2). At 0 and 28 days, faecal samples were obtained from four piglets per pen for microbiological analyses and serum samples were collected from two piglets per pen for serum metabolic profiling. Treatments significantly reduced diarrhoea occurrence and decreased the average faecal score (0.94 ± 0.08 CTRL, 0.31 ± 0.08 PLA, 0.45 ± 0.08 REU, 0.27 ± 0.08 P+R; p< 0.05). The PLA group registered the lowest number of diarrhoea cases compared to other groups (20 cases CTRL, 5 cases PLA, 8 cases REU, 10 cases P+R; p< 0.01). After 28 days, the globulin serum level increased in PLA compared to the other groups (24.91 ± 1.09 g/L CTRL, 28.89 ± 1.03 g/L PLA, 25.91 ± 1.03 g/L REU, 25.31 ± 1.03 g/L P+R; p< 0.05). L. plantarum and L. reuteri could thus be considered as interesting functional additives to prevent diarrhoea occurrence in weaned piglets.

ACS Style

Matteo Dell’Anno; Maria Callegari; Serena Reggi; Valentina Caprarulo; Carlotta Giromini; Ambra Spalletta; Simona Coranelli; Carlo Sgoifo Rossi; Luciana Rossi. Lactobacillus plantarum and Lactobacillus reuteri as Functional Feed Additives to Prevent Diarrhoea in Weaned Piglets. Animals 2021, 11, 1766 .

AMA Style

Matteo Dell’Anno, Maria Callegari, Serena Reggi, Valentina Caprarulo, Carlotta Giromini, Ambra Spalletta, Simona Coranelli, Carlo Sgoifo Rossi, Luciana Rossi. Lactobacillus plantarum and Lactobacillus reuteri as Functional Feed Additives to Prevent Diarrhoea in Weaned Piglets. Animals. 2021; 11 (6):1766.

Chicago/Turabian Style

Matteo Dell’Anno; Maria Callegari; Serena Reggi; Valentina Caprarulo; Carlotta Giromini; Ambra Spalletta; Simona Coranelli; Carlo Sgoifo Rossi; Luciana Rossi. 2021. "Lactobacillus plantarum and Lactobacillus reuteri as Functional Feed Additives to Prevent Diarrhoea in Weaned Piglets." Animals 11, no. 6: 1766.

Journal article
Published: 06 June 2021 in Animals
Reads 0
Downloads 0

The effects of the dietary administration of a combination of Quebracho and Chestnut tannins, leonardite and tributyrin were evaluated in weaned piglets. A total of 168 weaned piglets (Landrace × Large White) were randomly allotted to two experimental groups (6 pens/group, 14 piglets/pen). Animals were fed a basal control diet (CTRL) and a treatment diet (MIX) supplemented with 0.75% tannin extracts, 0.25% leonardite and 0.20% tributyrin for 28 days. Individual body weight and feed intake were recorded weekly. Diarrhoea incidence was recorded by a faecal scoring scale (0–3; considering diarrhoea ≥ 2). At 0 and 28 days, faecal samples were obtained from four piglets/pen for microbiological and chemical analyses of faecal microbiota, which were then assessed by V3-V4 region amplification sequencing. At 28 days, blood from two piglets/pen was sampled to evaluate the serum metabolic profile. After 28 days, a reduction in diarrhoea incidence was observed in the MIX compared to CTRL group (p< 0.05). In addition, compared to CTRL, MIX showed a higher lactobacilli:coliform ratio and increased Prevotella and Fibrobacter genera presence (p< 0.01). The serum metabolic profile showed a decreased level of low-density lipoproteins in the treated group (p< 0.05). In conclusion, a combination of tannin extract, leonardite and tributyrin could decrease diarrhoea incidence and modulate the gut microbiota.

ACS Style

Matteo Dell’Anno; Serena Reggi; Valentina Caprarulo; Monika Hejna; Carlo Sgoifo Rossi; Maria Callegari; Antonella Baldi; Luciana Rossi. Evaluation of Tannin Extracts, Leonardite and Tributyrin Supplementation on Diarrhoea Incidence and Gut Microbiota of Weaned Piglets. Animals 2021, 11, 1693 .

AMA Style

Matteo Dell’Anno, Serena Reggi, Valentina Caprarulo, Monika Hejna, Carlo Sgoifo Rossi, Maria Callegari, Antonella Baldi, Luciana Rossi. Evaluation of Tannin Extracts, Leonardite and Tributyrin Supplementation on Diarrhoea Incidence and Gut Microbiota of Weaned Piglets. Animals. 2021; 11 (6):1693.

Chicago/Turabian Style

Matteo Dell’Anno; Serena Reggi; Valentina Caprarulo; Monika Hejna; Carlo Sgoifo Rossi; Maria Callegari; Antonella Baldi; Luciana Rossi. 2021. "Evaluation of Tannin Extracts, Leonardite and Tributyrin Supplementation on Diarrhoea Incidence and Gut Microbiota of Weaned Piglets." Animals 11, no. 6: 1693.

Journal article
Published: 22 October 2020 in Animals
Reads 0
Downloads 0

In pig livestock, alternatives to in-feed antibiotics are needed to control enteric infections. Plant extracts such as tannins can represent an alternative as a natural source of functional compounds. The aim of this study was to evaluate the in vitro digestibility and in vivo effects of oral supplementation of combined chestnut (Ch) and quebracho (Qu) tannins in order to establish if they can induce a positive effect on weaned piglets’ performance, metabolic status and fecal parameters. In vitro digestibility (dry matter, DM) of diets was calculated using a multi-step enzymatic technique. In vitro digested diet samples were further tested on an intestinal porcine enterocyte cell line (IPEC-J2). Weaned piglets (n = 120; 28 ± 2 day old) were randomly allotted to two groups (12 pens in total with 10 pigs per pen): control (Ctrl) and treatment (Ch/Qu). After one week of adaptation (day 0), 35-day-old piglets in the Ctrl group were fed a Ctrl diet and the Ch/Qu group were fed with 1.25% Ch/Qu for 40 days. Body weight and feed intake per pen were recorded weekly. At day 40, blood and fecal samples were collected. Principal metabolic parameters were evaluated from blood samples by enzymatic colorimetric analysis. Total phenolic compounds, urea, and ammonia in feces were analyzed (Megazyme International, Bray, Ireland). In vitro digestibility and cell viability assays showed that the inclusion of 1.25% Ch/Qu slightly reduced diet digestibility compared with the Ctrl diet, while intestinal cell viability was not altered with low concentrations of Ch/Qu digesta compared with Ctrl. In vivo results did not show any adverse effects of Ch/Qu on feed intake and growth performance, confirming that dietary inclusion of Ch/Qu at a concentration of 1.25% did not impair animal performance. The decreased diet DM digestibility in the Ch/Qu diet may cause increased serum concentration of albumin (Ctrl: 19.30 ± 0.88; Ch/Qu: 23.05 ± 0.88) and albumin/globulin ratio (Ctrl: 0.58 ± 0.04; Ch/Qu: 0.82 ± 0.04), but decreased creatinine (Ctrl: 78.92 ± 4.18; Ch/Qu: 54.82 ± 4.18) and urea (Ctrl: 2.18 ± 0.19; Ch/Qu: 0.95 ± 0.19) compared with Ctrl. Pigs in the Ch/Qu group contained higher (p < 0.05) concentrations of fecal phenolic compounds and nitrogen than the Ctrl group, while fecal ammonia and urea were not affected by tannins. In conclusion, Ch/Qu tannin supplementation did not influence growth performance. Although lower digestibility was observed in the diet supplemented with Ch/Qu tannins, Ch/Qu supplementation did not show any adverse effect on intestinal epithelial cell viability.

ACS Style

Valentina Caprarulo; Monika Hejna; Carlotta Giromini; Yanhong Liu; Matteo Dell’Anno; Stefania Sotira; Serena Reggi; Carlo Sgoifo-Rossi; Maria Callegari; Luciana Rossi. Evaluation of Dietary Administration of Chestnut and Quebracho Tannins on Growth, Serum Metabolites and Fecal Parameters of Weaned Piglets. Animals 2020, 10, 1945 .

AMA Style

Valentina Caprarulo, Monika Hejna, Carlotta Giromini, Yanhong Liu, Matteo Dell’Anno, Stefania Sotira, Serena Reggi, Carlo Sgoifo-Rossi, Maria Callegari, Luciana Rossi. Evaluation of Dietary Administration of Chestnut and Quebracho Tannins on Growth, Serum Metabolites and Fecal Parameters of Weaned Piglets. Animals. 2020; 10 (11):1945.

Chicago/Turabian Style

Valentina Caprarulo; Monika Hejna; Carlotta Giromini; Yanhong Liu; Matteo Dell’Anno; Stefania Sotira; Serena Reggi; Carlo Sgoifo-Rossi; Maria Callegari; Luciana Rossi. 2020. "Evaluation of Dietary Administration of Chestnut and Quebracho Tannins on Growth, Serum Metabolites and Fecal Parameters of Weaned Piglets." Animals 10, no. 11: 1945.

Journal article
Published: 23 January 2020 in Animals
Reads 0
Downloads 0

Castanea sativa Mill. (Fagaceae) is the predominant sweet chestnut tree in Europe. Despite the significant economic value of chestnuts as sources of food and wood, the high content of tannin also increases the value of sweet chestnut trees. Quebracho trees (Schinopsis spp., family Anacardiaceae) grow mainly in Argentina and Paraguay. Quebracho extract obtained from Schinopsis spp. contains 15% to 21% pure tannin. Tannins extracted from these plants have been applied in intensive swine farms due to their ability to improve animal performance and health. However, there are contrasting results regarding the bio-accessibility of chestnut and quebracho and their relative antioxidant activity and growth-rate reducing ability on E. coli, which ultimately affect their benefits in terms of intestinal health and animal production. Our results demonstrate that chestnut and quebracho exert a growth inhibitory activity against Enterotoxigenic E. coli (ETEC) species and antioxidant capacity directly, without extraction and after in vitro digestion. Our findings not only suggest that the combined use of chestnut and quebracho can maximize their functional effects, but also that an appropriate dosage of tannins may be key in terms of their effect on bacteria and cells. Quebracho (Qu) and chestnut (Ch) are natural sources of tannins and they are currently used in animal nutrition as feed ingredients. However, to date the bio-accessibility, antimicrobial, antioxidant, and intestinal epithelial cell stimulatory doses of Qu and Ch have not been determined. Our study investigates the antioxidant and E. coli F4+ and F18+ growth inhibitory activity of Qu, Ch, and their combinations after solubilization in water (to evaluate the already bio-accessible molecules) and after simulated gastro-intestinal digestion in vitro. The effect of an in vitro digested Ch and Qu combination was also tested on intestinal epithelial IPEC-J2 cells experimentally stressed with hydrogen peroxide (H2O2) and Dextran Sodium Sulfate (DSS). The results showed that undigested Qu and Ch alone, and in combination, exerted a valuable antioxidant capacity and E. coli F4+ and F18+ growth inhibitory activity. The concentration of 1200 µg/mL exhibited the highest E. coli growth inhibitory activity for all the samples tested. In addition, after in vitro digestion, Qu and Qu50%–Ch50% maintained E. coli growth inhibitory activity and a modest antioxidant capacity. Three hours pre-treatment with in vitro digested Qu50%–Ch50% counteracted the H2O2 and DSS experimentally-induced stress in the intestinal IPEC-J2 cells. Ch and Qu tannin extracts, particularly when combined, may exert E. coli F4+ and F18+ growth inhibitory activity and valuable antioxidant and cell viability modulation activities.

ACS Style

Serena Reggi; Carlotta Giromini; Matteo Dell’Anno; Antonella Baldi; Raffaella Rebucci; Luciana Rossi. In Vitro Digestion of Chestnut and Quebracho Tannin Extracts: Antimicrobial Effect, Antioxidant Capacity and Cytomodulatory Activity in Swine Intestinal IPEC-J2 Cells. Animals 2020, 10, 195 .

AMA Style

Serena Reggi, Carlotta Giromini, Matteo Dell’Anno, Antonella Baldi, Raffaella Rebucci, Luciana Rossi. In Vitro Digestion of Chestnut and Quebracho Tannin Extracts: Antimicrobial Effect, Antioxidant Capacity and Cytomodulatory Activity in Swine Intestinal IPEC-J2 Cells. Animals. 2020; 10 (2):195.

Chicago/Turabian Style

Serena Reggi; Carlotta Giromini; Matteo Dell’Anno; Antonella Baldi; Raffaella Rebucci; Luciana Rossi. 2020. "In Vitro Digestion of Chestnut and Quebracho Tannin Extracts: Antimicrobial Effect, Antioxidant Capacity and Cytomodulatory Activity in Swine Intestinal IPEC-J2 Cells." Animals 10, no. 2: 195.

Journal article
Published: 12 June 2018 in International Journal of Cardiology
Reads 0
Downloads 0

Background Atherosclerosis is a slowly progressing, chronic multifactorial disease characterized by the accumulation of lipids, inflammatory cells, and fibrous tissue that drives to the formation of asymmetric focal thickenings in the tunica intima of large and mid-sized arteries. Despite the high therapeutic potential of ApoA-1 proteins, the purification and delivery into the disordered organisms of these drugs is still limited by low efficiency in these processes. Methods and results We report here a novel production and delivery system of anti-atherogenic APOA-1Milano muteins (APOA-1M) by means of genetically modified rice plants. APOA-1M, delivered as protein extracts from transgenic rice seeds, significantly reduced macrophage activation and foam cell formation in vitro in oxLDL-loaded THP-1 model. The APOA-1M delivery method and therapeutic efficacy was tested in healthy mice and in Apoe −/− mice fed with high cholesterol diet (Western Diet, WD). APOA-1M rice milk significantly reduced atherosclerotic plaque size and lipids composition in aortic sinus and aortic arch of WD-fed Apoe −/− mice as compared to wild type rice milk-treated, WD-fed Apoe −/− mice. APOA-1M rice milk also significantly reduced macrophage number in liver of WD-fed Apoe −/− mice as compared to WT rice milk treated mice. Translational impact The delivery of therapeutic APOA-1M full length proteins via oral administration of rice seeds protein extracts (the ‘rice milk') to the disordered organism, without any need of purification, might overcome the main APOA1-based therapies' limitations and improve the use of this molecules as therapeutic agents for cardiovascular patients.

ACS Style

Gabriele Romano; Serena Reggi; Barbara Kutryb-Zajac; Amanda Facoetti; Elisa Chisci; Mariateresa Pettinato; Maria Rita Giuffrè; Federica Vecchio; Silvia Leoni; Marco De Giorgi; Federica Avezza; Massimiliano Cadamuro; Luca Crippa; Biagio Eugenio Leone; Marialuisa Lavitrano; Ilaria Rivolta; Donatella Barisani; Ryszard Smolenski; Roberto Giovannoni. APOA-1Milano muteins, orally delivered via genetically modified rice, show anti-atherogenic and anti-inflammatory properties in vitro and in Apoe atherosclerotic mice. International Journal of Cardiology 2018, 271, 233 -239.

AMA Style

Gabriele Romano, Serena Reggi, Barbara Kutryb-Zajac, Amanda Facoetti, Elisa Chisci, Mariateresa Pettinato, Maria Rita Giuffrè, Federica Vecchio, Silvia Leoni, Marco De Giorgi, Federica Avezza, Massimiliano Cadamuro, Luca Crippa, Biagio Eugenio Leone, Marialuisa Lavitrano, Ilaria Rivolta, Donatella Barisani, Ryszard Smolenski, Roberto Giovannoni. APOA-1Milano muteins, orally delivered via genetically modified rice, show anti-atherogenic and anti-inflammatory properties in vitro and in Apoe atherosclerotic mice. International Journal of Cardiology. 2018; 271 ():233-239.

Chicago/Turabian Style

Gabriele Romano; Serena Reggi; Barbara Kutryb-Zajac; Amanda Facoetti; Elisa Chisci; Mariateresa Pettinato; Maria Rita Giuffrè; Federica Vecchio; Silvia Leoni; Marco De Giorgi; Federica Avezza; Massimiliano Cadamuro; Luca Crippa; Biagio Eugenio Leone; Marialuisa Lavitrano; Ilaria Rivolta; Donatella Barisani; Ryszard Smolenski; Roberto Giovannoni. 2018. "APOA-1Milano muteins, orally delivered via genetically modified rice, show anti-atherogenic and anti-inflammatory properties in vitro and in Apoe atherosclerotic mice." International Journal of Cardiology 271, no. : 233-239.

Journal article
Published: 19 November 2013 in Veterinary Research Communications
Reads 0
Downloads 0

The use of transgenic plants as delivery system for antigenic proteins is attractive for its simplicity and increases likelihood for local immune response at sites of infection. The aim of this study was to evaluate the protective effect of oral administration of tobacco seeds, expressing the FedA, the major protein of the F18 adhesive fimbriae, and B subunit of verocytotoxin, against verocytotoxin-producing E. coli (VTEC) strain in piglets. Forty-three early weaned piglets, were randomly divided into 4 experimental groups: 3 test groups and a control. Treatment groups orally received a bolus, with different dose of tobacco seeds on 0, 1, 2, 14 days post primary administration. After challenge, with 1*10(10) CFU of O138 Escherichia coli strain, piglets showed clinical scores significantly higher in the control group compared to orally immunized groups (P < 0.05) and the latter showed a faster recovery than in CG. In conclusion, oral administration of recombinant tobacco seeds expressing antigenic proteins against VTEC strains can induce a protective effect against challenger strain in piglets.

ACS Style

Luciana Rossi; Vittorio Dell’Orto; Simona Vagni; Vittorio Sala; Serena Reggi; Antonella Baldi. Protective effect of oral administration of transgenic tobacco seeds against verocytotoxic Escherichia coli strain in piglets. Veterinary Research Communications 2013, 38, 39 -49.

AMA Style

Luciana Rossi, Vittorio Dell’Orto, Simona Vagni, Vittorio Sala, Serena Reggi, Antonella Baldi. Protective effect of oral administration of transgenic tobacco seeds against verocytotoxic Escherichia coli strain in piglets. Veterinary Research Communications. 2013; 38 (1):39-49.

Chicago/Turabian Style

Luciana Rossi; Vittorio Dell’Orto; Simona Vagni; Vittorio Sala; Serena Reggi; Antonella Baldi. 2013. "Protective effect of oral administration of transgenic tobacco seeds against verocytotoxic Escherichia coli strain in piglets." Veterinary Research Communications 38, no. 1: 39-49.

Journal article
Published: 01 January 2013 in Journal of Veterinary Science
Reads 0
Downloads 0

Verocytotoxic Escherichia (E.) coli strains are responsible for swine oedema disease, which is an enterotoxaemia that causes economic losses in the pig industry. The production of a vaccine for oral administration in transgenic seeds could be an efficient system to stimulate local immunity. This study was conducted to transform tobacco plants for the seed-specific expression of antigenic proteins from a porcine verocytotoxic E. coli strain. Parameters related to an immunological response and possible adverse effects on the oral administration of obtained tobacco seeds were evaluated in a mouse model. Tobacco was transformed via Agrobacteium tumefaciens with chimeric constructs containing structural parts of the major subunit FedA of the F18 adhesive fimbriae and VT2e B-subunit genes under control of a seed specific GLOB promoter. We showed that the foreign Vt2e-B and F18 genes were stably accumulated in storage tissue by the immunostaining method. In addition, Balb-C mice receiving transgenic tobacco seeds via the oral route showed a significant increase in IgA-positive plasma cell presence in tunica propria when compared to the control group with no observed adverse effects. Our findings encourage future studies focusing on swine for evaluation of the protective effects of transformed tobacco seeds against E. coli infection

ACS Style

Luciana Rossi; Alessia DI Giancamillo; Serena Reggi; Cinzia Domeneghini; Antonella Baldi; Vittorio Sala; Vittorio Dell'orto; Annelies Coddens; Eric Cox; Corrado Fogher. Expression of verocytotoxicEscherichia coliantigens in tobacco seeds and evaluation of gut immunity after oral administration in mouse model. Journal of Veterinary Science 2013, 14, 263 -270.

AMA Style

Luciana Rossi, Alessia DI Giancamillo, Serena Reggi, Cinzia Domeneghini, Antonella Baldi, Vittorio Sala, Vittorio Dell'orto, Annelies Coddens, Eric Cox, Corrado Fogher. Expression of verocytotoxicEscherichia coliantigens in tobacco seeds and evaluation of gut immunity after oral administration in mouse model. Journal of Veterinary Science. 2013; 14 (3):263-270.

Chicago/Turabian Style

Luciana Rossi; Alessia DI Giancamillo; Serena Reggi; Cinzia Domeneghini; Antonella Baldi; Vittorio Sala; Vittorio Dell'orto; Annelies Coddens; Eric Cox; Corrado Fogher. 2013. "Expression of verocytotoxicEscherichia coliantigens in tobacco seeds and evaluation of gut immunity after oral administration in mouse model." Journal of Veterinary Science 14, no. 3: 263-270.

Short communication
Published: 31 July 2009 in Plant Physiology and Biochemistry
Reads 0
Downloads 0

Sirtuins are known as regulators of age-dependent gene transcription and chromatin modification in yeast and in animals, but information about their occurrence and role in plants is scarce. Sirtuin-like sequences were amplified using two highly degenerate primers designed comparing sirtuin sequences of seven different plant species, and characterised at the sequence level in Vitis vinifera L. cv. Barbera clone R4. The data were confirmed and analysed by searching the published database from two independent grape genome projects with the obtained sequences. Two putative sirtuin genes containing sirtuin-conserved domains were found on chromosome 7 and on chromosome 19, suggesting the occurrence of sirtuins in grapevine.

ACS Style

Matteo Busconi; Serena Reggi; Corrado Fogher; Luigi Bavaresco. Evidence of a sirtuin gene family in grapevine (Vitis vinifera L.). Plant Physiology and Biochemistry 2009, 47, 650 -652.

AMA Style

Matteo Busconi, Serena Reggi, Corrado Fogher, Luigi Bavaresco. Evidence of a sirtuin gene family in grapevine (Vitis vinifera L.). Plant Physiology and Biochemistry. 2009; 47 (7):650-652.

Chicago/Turabian Style

Matteo Busconi; Serena Reggi; Corrado Fogher; Luigi Bavaresco. 2009. "Evidence of a sirtuin gene family in grapevine (Vitis vinifera L.)." Plant Physiology and Biochemistry 47, no. 7: 650-652.

Evaluation study
Published: 13 January 2008 in Antonie van Leeuwenhoek
Reads 0
Downloads 0

Amplified fragment length polymorphism (AFLPs) were used to analyse the naturally occurring flora of lactic acid bacteria (LAB) in gastrointestinal tracts of two healthy 65-day-old calves. More than 1,000 of presumptive LAB were collected and cultured from the gastrointestinal tracts and, among the isolated colonies, a total of 311 strains were analysed and separated into eight clusters based on AFLP banding patterns. To precisely determine the species inside the clusters, partial sequences of fragments of the 16S ribosomal DNA gene were determined, and sequence homology searches were conducted through GenBank on few strains per cluster. The most representative genera of LAB were Lactobacillus (169 isolates, 54% of total) and Streptococcus (99 isolates, 32% of total), while the most frequent species was identified as L. mucosae with 86 different isolates (51% of the Lactobacillus spp. and 28% of the total). This report gives a first characterization of LAB strain biodiversity recovered directly from calf intestine and is the first account of the presence of the L. mucosae species in calves. Moreover it demonstrates that the AFLP is a robust and useful technique for characterizing the strain level of LAB microflora.

ACS Style

Matteo Busconi; Serena Reggi; Corrado Fogher. Evaluation of biodiversity of lactic acid bacteria microbiota in the calf intestinal tracts. Antonie van Leeuwenhoek 2008, 94, 145 -155.

AMA Style

Matteo Busconi, Serena Reggi, Corrado Fogher. Evaluation of biodiversity of lactic acid bacteria microbiota in the calf intestinal tracts. Antonie van Leeuwenhoek. 2008; 94 (2):145-155.

Chicago/Turabian Style

Matteo Busconi; Serena Reggi; Corrado Fogher. 2008. "Evaluation of biodiversity of lactic acid bacteria microbiota in the calf intestinal tracts." Antonie van Leeuwenhoek 94, no. 2: 145-155.

Journal article
Published: 01 April 2006 in Molecular Breeding
Reads 0
Downloads 0

Expression of the vhb gene, encoding the hemoglobin protein from Vitreoscilla spp. (VHb), has been shown to increase cell growth and protein synthesis, modify the oxygen-dependent product biosynthesis and the susceptibility to oxidative and nitrosative stresses in several host microrganisms, and to improve plant tolerance to flooding-submergence. A chimeric construct consisting of the CaMV35S promoter fused to the vhb gene and nopaline synthase terminator was transferred into white poplar (Populus alba L.) via Agrobacterium tumefaciens in order to test the generality of these phenomena. The presence of the vhb gene was demonstrated by Southern blot analysis. Accumulation of the vhb transcript and protein was detected in all the selected transgenic poplar lines. In vitro growth bioassays revealed that the vhb gene expression in transgenic poplar plants did not significantly affect their growth pattern. One out of the six selected transgenic lines showed significantly higher values for plant height and stem biomass in greenhouse conditions and exhibited enhancement of root biomass production and stem diameter when compared to the wild-type plants. However, no significant differences in chlorophyll a, b, total carotenoid and protein contents were observed. Two selected transgenic lines were characterized in more detail for tolerance to submergence, oxidative and nitrosative stresses. Under in vitro and in vivo submergence conditions, growth parameters and total protein content of transgenic VHb poplars were similar to those observed in the wild-type plants. In addition, leaf discs from the transgenic plants maintained in standard growth conditions did not reveal increased tolerance to oxidative stress by hydrogen peroxide compared to wild-type plants. In a parallel study, cell suspension cultures obtained from both wild-type and VHb transgenic lines were evaluated for growth and survival in the presence of oxidative and nitrosative stresses. No significant differences were observed between the tested VHb and wild-type poplar lines. Our results show that VHb expression in plants can have erratic effects since the enhancement of plant growth and biomass production and the tolerance to submergence, oxidative and nitrosative stresses are not consistently dependent on the presence of this specific function. Consequently, the genetic manipulation of plant oxygen metabolism must be carefully evaluated and extensive biochemical, molecular and cellular investigations are required to assess the real value of the final products.

ACS Style

S. Zelasco; S. Reggi; P. Calligari; Alma Balestrazzi; C. Bongiorni; E. Quattrini; G. Delia; S. Bisoffi; C. Fogher; M. Confalonieri. Expression of the Vitreoscilla Hemoglobin (VHb)-Encoding Gene in Transgenic White Poplar: Plant Growth and Biomass Production, Biochemical Characterization and Cell Survival under Submergence, Oxidative and Nitrosative Stress Conditions. Molecular Breeding 2006, 17, 201 -216.

AMA Style

S. Zelasco, S. Reggi, P. Calligari, Alma Balestrazzi, C. Bongiorni, E. Quattrini, G. Delia, S. Bisoffi, C. Fogher, M. Confalonieri. Expression of the Vitreoscilla Hemoglobin (VHb)-Encoding Gene in Transgenic White Poplar: Plant Growth and Biomass Production, Biochemical Characterization and Cell Survival under Submergence, Oxidative and Nitrosative Stress Conditions. Molecular Breeding. 2006; 17 (3):201-216.

Chicago/Turabian Style

S. Zelasco; S. Reggi; P. Calligari; Alma Balestrazzi; C. Bongiorni; E. Quattrini; G. Delia; S. Bisoffi; C. Fogher; M. Confalonieri. 2006. "Expression of the Vitreoscilla Hemoglobin (VHb)-Encoding Gene in Transgenic White Poplar: Plant Growth and Biomass Production, Biochemical Characterization and Cell Survival under Submergence, Oxidative and Nitrosative Stress Conditions." Molecular Breeding 17, no. 3: 201-216.

Journal article
Published: 01 January 2005 in Plant Molecular Biology
Reads 0
Downloads 0

Gaucher disease, the most common genetic lysosomal disorder, is caused by the lack of functional acid β-glucosidase (GCase) and is currently treated at a very high cost by enzyme replacement therapy. In an attempt to provide a safe and cost-effective production system, human placental GCase was produced and purified from transgenic tobacco seeds. Plant-derived recombinant GCase was found to be enzymatically active, uptaken by human fibroblasts and free of immunogenic xylose and fucose residues. This report demonstrates the potential of plant bioreactors in the large-scale production of injectable proteins required for lifelong therapy.

ACS Style

Serena Reggi; Stefano Marchetti; Tamara Patti; Francesca De Amicis; Roberta Cariati; Bruno Bembi; Corrado Fogher. Recombinant human acid ?-glucosidase stored in tobacco seed is stable, active and taken up by human fibroblasts. Plant Molecular Biology 2005, 57, 101 -113.

AMA Style

Serena Reggi, Stefano Marchetti, Tamara Patti, Francesca De Amicis, Roberta Cariati, Bruno Bembi, Corrado Fogher. Recombinant human acid ?-glucosidase stored in tobacco seed is stable, active and taken up by human fibroblasts. Plant Molecular Biology. 2005; 57 (1):101-113.

Chicago/Turabian Style

Serena Reggi; Stefano Marchetti; Tamara Patti; Francesca De Amicis; Roberta Cariati; Bruno Bembi; Corrado Fogher. 2005. "Recombinant human acid ?-glucosidase stored in tobacco seed is stable, active and taken up by human fibroblasts." Plant Molecular Biology 57, no. 1: 101-113.