Summary: Plant phenotyping forms the core of crop breeding, allowing breeders to build on physiological traits and mechanistic science to inform their selection of material for crossing and genetic gain. Recent rapid progress in high‐throughput techniques based on machine vision, robotics, and computing (plant phenomics) enables crop physiologists and breeders to quantitatively measure complex and previously intractable traits. By combining these techniques with affordable genomic sequencing and genotyping, machine learning, and genome selection approaches, breeders have an opportunity to make rapid genetic progress. This review focuses on how field‐based plant phenomics can enable next‐generation physiological breeding in cereal crops for traits related to radiation use efficiency, photosynthesis, and crop biomass. These traits have previously been regarded as difficult and laborious to measure but have recently become a focus as cereal breeders find genetic progress from ‘Green Revolution’ traits such as harvest index become exhausted. Application of LiDAR, thermal imaging, leaf and canopy spectral reflectance, Chl fluorescence, and machine learning are discussed using wheat and sorghum phenotyping as case studies. A vision of how crop genomics and high‐throughput phenotyping could enable the next generation of crop research and breeding is presented.

Accurate prediction of crop yield at the field scale is critical to addressing crop production challenges and reducing the impacts of climate variability and change. Recently released Sentinel-2 (S2) satellite data with a return cycle of five days and a high resolution at 13 spectral bands allows close observation of crop phenology and crop physiological attributes at field scale during crop growth. Here, we test the potential for indices derived from S2 data to estimate dryland wheat yields at the field scale and the potential for enhanced predictability by incorporating a modelled crop water stress index (SI). Observations from 103 study fields over the 2016 and 2017 cropping seasons across Northeastern Australia were used. Vegetation indices derived from S2 showed moderately high accuracy in yield prediction and explained over 70% of the yield variability. Specifically, the red edge chlorophyll index (CI; chlorophyll) (R² = 0.76, RMSE = 0.88 t/ha) and the optimized soil-adjusted vegetation index (OSAVI; structural) (R² = 0.74, RMSE = 0.91 t/ha) showed the best correlation with field yields. Furthermore, combining the crop model-derived SI with both structural and chlorophyll indices significantly enhanced predictability. The best model with combined OSAVI, CI and SI generated a much higher correlation, with R² = 0.91 and RMSE = 0.54 t/ha. When validating the models on an independent set of fields, this model also showed high correlation (R² = 0.93, RMSE = 0.64 t/ha). This study demonstrates the potential of combining S2-derived indices and crop model-derived indices to construct an enhanced yield prediction model suitable for fields in diversified climate conditions.

Genetic improvement in sorghum breeding programs requires the assessment of adaptation traits in small-plot breeding trials across multiple environments. Many of these phenotypic assessments are made by manual measurement or visual scoring, both of which are time consuming and expensive. This limits trial size and the potential for genetic gain. In addition, these methods are typically restricted to point estimates of particular traits, such as leaf senescence or flowering and do not capture the dynamic nature of crop growth. In water-limited environments in particular, information on leaf area development over time would provide valuable insight into water use and adaptation to water scarcity during specific phenological stages of crop development. Current methods to estimate plant leaf area index (LAI) involve destructive sampling and are not practical in breeding. Unmanned aerial vehicles (UAV) and proximal-sensing technologies open new opportunities to assess these traits multiple times in large small-plot trials. We analyzed vegetation-specific crop indices obtained from a narrowband multi-spectral camera on board a UAV platform flown over a small pilot trial with 30 plots (10 genotypes randomized within 3 blocks). Due to variable emergence we were able to assess the utility of these vegetation indices to estimate canopy cover and LAI over a large range of plant densities. We found good correlations between the Normalized Difference Vegetation Index (NDVI) and the Enhanced Vegetation Index (EVI) with plant number per plot, canopy cover and LAI both during the vegetative growth phase (pre-anthesis) and at maximum canopy cover shortly after anthesis. We also analyzed the utility of time-sequence data to assess the senescence pattern of sorghum genotypes known as fast (senescent) or slow senescing (stay-green) types. The Normalized Difference Red Edge (NDRE) index which estimates leaf chlorophyll content was most useful in characterizing the leaf area dynamics/senescence patterns of contrasting genotypes. These methods to monitor dynamics of green and senesced leaf area are suitable for out-scaling to enhance phenotyping of additional crop canopy characteristics and likely crop yield responses among genotypes across large fields and multiple dates.