Plumeria alba Linn., a member of the Apocynaceae family, is a widely recognized medicinal plant known for its diverse pharmacological properties. The present study focuses on the pharmacognostical and phytochemical evaluation of Plumeria alba, along with its antimicrobial potential and application in the development of a topical herbal cream. Pharmacognostical studies, including macroscopic, microscopic, and physicochemical evaluations, play a crucial role in the identification, authentication, and standardization of the plant material. Phytochemical analysis reveals the presence of bioactive constituents such as flavonoids, tannins, phenolic compounds, alkaloids, saponins, and glycosides, which are responsible for its therapeutic activities. The plant exhibits significant antibacterial and antifungal activity against various pathogenic microorganisms, supporting its traditional use in treating skin infections and wounds. Based on these properties, a topical herbal cream formulation containing Plumeria alba extract was developed using suitable excipients. The formulated cream was evaluated for parameters such as pH, viscosity, spreadability, homogeneity, stability, and antimicrobial activity. The results suggest that Plumeria alba possesses promising potential as a natural antimicrobial agent and can be effectively utilized in topical herbal formulations for dermatological applications. Further research and clinical studies are recommended to validate its efficacy and safety.