Marco Marradi

Prof. Marco Marradi

Università degli Studi di Firenze, Florence, Tuscany, Italy
orcid

Research Focus & Metrics

Academic Career Length

26Years (2001-Present)

(Calculated once Scilit publication data is synced: current year − year of first publication + 1)

Representative Publications

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19 December 2012 in Chemical Society Reviews
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Journal Article
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ABAnna Bernardi
Anna Bernardi
Jesus Jimenez-BarberoJesus Jimenez-Barbero
Jesus Jimenez-Barbero
ACAlessandro Casnati
Alessandro Casnati
Cristina De CastroCristina De Castro
Cristina De Castro
TDTamis Darbre
Tamis Darbre
AIAnne Imberty
Anne Imberty
Multivalency plays a major role in biological processes and particularly in the relationship between pathogenic microorganisms and their host that involves protein–glycan recognition. These interactions occur during the first steps of infection, for specific recognition between host and bacteria, but also at different stages of the immune response. The search for high-affinity ligands for studying such interactions involves the combination of carbohydrate head groups with different scaffolds and linkers generating multivalent glycocompounds with controlled spatial and topology parameters. By interfering with pathogen adhesion, such glycocompounds including glycopolymers, glycoclusters, glycodendrimers and glyconanoparticles have the potential to improve or replace antibiotic treatments that are now subverted by resistance. Multivalent glycoconjugates have also been used for stimulating the innate and adaptive immune systems, for example with carbohydrate-based vaccines. Bacteria present on their surfaces natural multivalent glycoconjugates such as lipopolysaccharides and S-layers that can also be exploited or targeted in anti-infectious strategies.

4 January 2013 in Chemical Society Reviews
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MMMarco Marradi
Marco Marradi
FCFabrizio Chiodo
Fabrizio Chiodo
IGIsabel García
Isabel García
SPSoledad Penadés
Soledad Penadés
The quest for the construction of multivalent carbohydrate systems, with precise geometries that are highly efficient in interacting with carbohydrate binding proteins, has been a goal of synthetic chemists since the discovery of the multivalent nature of carbohydrate-mediated interactions. However, the control of the spatial and topological requirements for these systems is still a challenge. Glyconanoparticles (GNPs) are sugar-coated gold, iron oxide or semiconductor nanoparticles with defined thiol-ending glycosides that combine the multivalent presentation of carbohydrates (glycoclusters) with the special chemico-physical properties of the nano-sized metallic core. The possibility of attaching different types of carbohydrates and other molecules (such as luminescent probes, peptides, and magnetic chelates) onto the same gold nanoparticle in a controlled way (multifunctional GNPs), as well as modifying the core in order to obtain glyconanoparticles with magnetic or fluorescence properties (multimodal GNPs) makes this multivalent glyco-scaffold suitable for carrying out studies on carbohydrate-mediated interactions and applications in molecular imaging. In this review, we focus mainly on the rational design of glyconanoparticles as scaffolds for combining different ligands and survey the most recent examples of glyconanoparticles as both multivalent carbohydrate systems and probes for molecular imaging.

23 September 2009 in Chemistry – A European Journal
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OMOlga Martínez‐Ávila
Olga Martínez‐Ávila
KHKarolin Hijazi
Karolin Hijazi
MMMarco Marradi
Marco Marradi
CCCaroline Clavel
Caroline Clavel
CCColin Campion
Colin Campion
SPSoledad Penadés
Soledad Penadés

25 May 2012 in Nanomedicine
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Journal Article
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DSDodi Safari
Dodi Safari
MMMarco Marradi
Marco Marradi
FCFabrizio Chiodo
Fabrizio Chiodo
HDHuberta A Th Dekker
Huberta A Th Dekker
YSYulong Shan
Yulong Shan
HSHarm Snippe
Harm Snippe
Coupling of capsular polysaccharides of pathogens to immunogenic protein carriers (conjugate vaccines) improves carbohydrate immune response. Our idea is to explore gold nanoclusters as carriers to prepare fully synthetic carbohydrate vaccines. Gold glyconanoparticles bearing a synthetic tetrasaccharide epitope related to the Streptococcus pneumoniae type 14 capsular polysaccharide (Pn14PS), the T-helper ovalbumin 323-339 peptide (OVA(323-339)), and D-glucose were prepared by a one-pot method. Their immunogenicity was tested in mice. Cytokine levels after spleen cell stimulation with OVA(323-339) were analyzed using a luminex-multiplex cytokine assay. The capacity of the evoked antibodies to promote the uptake of S. pneumoniae type 14 by leukocytes was assessed. Glyconanoparticles containing 45% of tetrasaccharide and 5% OVA(323-339) triggered specific anti-Pn14PS IgG antibodies. Cytokine levels confirmed that glyconanoparticles led to T-helper cell activation. The anti-saccharide antibodies promoted the phagocytosis of type 14 bacteria by human leukocytes, indicating the functionality of the antibodies. Gold nanoparticles have great potential as carriers for the development of a great diversity of fully synthetic carbohydrate-based vaccines.

1 May 2010 in Bioorganic & Medicinal Chemistry Letters
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Journal Article
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PGPaolo Di Gianvincenzo
Paolo Di Gianvincenzo
MMMarco Marradi
Marco Marradi
OMOlga María Martínez-Ávila
Olga María Martínez-Ávila
LBLuis Miguel Bedoya
Luis Miguel Bedoya
JAJosé Alcami
José Alcami
SPSoledad Penadés
Soledad Penadés

27 May 2008 in European Journal of Organic Chemistry
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Journal Article
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PMPedro Merino
Pedro Merino
IDIgnacio Delso
Ignacio Delso
TTTomás Tejero
Tomás Tejero
Francesca CardonaFrancesca Cardona
Francesca Cardona
MMMarco Marradi
Marco Marradi
Andrea GotiAndrea Goti
Andrea Goti