The present study aims to evaluate the potential for the pollution of the environment by two herbicides (quizalofop-p-ethyl and cycloxydim), using the Allium test. The species in question is Allium cepa (onion, 2n = 16), one of the most common plant indicators of environmental pollution. The working method consisted of obtaining the meristematic roots of Allium cepa and their treatment with herbicides at three different concentrations (0.5%, 1%, and 1.5%) for each herbicide for 24 h, for comparison with an untreated control. The results obtained from the cytological study indicated a strong cytotoxic and genotoxic effect for both herbicides, but especially for quizalofop-p-ethyl, where the mitotic index decreased from 30.2% (control) to 9.6% for the variant treated with 1.5% herbicide. In this case, a strong mitodepressive effect was shown by a highly significant percentage (35.4%) of chromosomal aberrations and nuclear alterations: stickiness, fragments, C-mitosis, lobulated nucleus, micronuclei, and nuclear erosion. The mitodepressive effect as well as the percentage of chromosomal aberrations increased with a higher herbicide concentration. The obtained results suggest the strong potential for pollution of the two herbicides, particularly at concentrations higher than 0.5%; therefore, we recommend caution in their use to avoid undesirable effects on the environment.

The aim of this work was to study how certain applied cropping systems (conventional systems differentiated by fertilization level or sowing season and subsistence farming) influence yield, quality, productivity elements, and morphological characters in a collection of Romanian and foreign wheat cultivars. The following indicators were evaluated: productive potential (yield), quality (test weight, protein content, wet gluten content, deformation index, sedimentation index, and gluten index), as well as other elements that determine yield (number of ears/square meter, thousand kernel weight, number of grains/ear, and weight of grains/ear) and plant height. The results show that the cropping systems influenced all the elements studied except the thousand-kernel weight. The only characteristics influenced by higher nitrogen fertilization were test weight, protein content, wet gluten content, deformation index, and gluten index. The superiority of a delayed conventional system was shown by the number of grains/wheat ear and the deformation index. Protein content was differentiated between the conventional and the subsistence system, but especially between the low-input and the conventional system. Nitrogen supply is the most important factor for determining wheat productivity and grain quality.

The aim of this paper is to trace genetically modified soybean in food and feed products present on the Romanian market by using molecular extraction, identification and quantification methodologies. Nine samples (3 food samples, 5 soybean samples and 1 soybean meal) were analysed using the classical and real-time polymerase chain reaction (PCR) method. DNA-genetically modified organism (GMO) was not detected in two of the three analysed samples (food products). However, it could be found in four samples ranging below the limit of 0.9%, and in three samples, above the limit of 0.9%. The results obtained through real-time PCR quantification show that DNA-RRS was detectable in different amounts in different samples: ranging between 0.27% and 9.36% in soy beans, and reaching 50.98% in soybean meal. The current research focuses on how products containing GMO above the limit (it is common knowledge that it is necessary to label the products containing more than 0.9% Genetically Modified DNA) are differentiated on the market with a view to labeling food and feed products in terms of the accidental presence of approved genetically modified plants. The benefits brought by genetic engineering in obtaining genetically modified organisms can be balanced with their public acceptance and with certain known or unknown risks that they can bring.

This study presents the variability of some biochemical indicators in the winter wheat assortments tested in south-western Oltenia (Romania) and identification of the sources showing a high antioxidant activity. The peroxidase activity has intensified as the stress induced by treatment with PEG of different concentrations and in different doses increased. Regarding the peroxidase content, among the varieties treated with PEG 10,000 25%, the majority of the Romanian varieties tested showed higher values of the PEG/control treatment ratio, which suggests tolerance to drought. In reverse, the activity of ascorbate peroxidase is lower in tolerant varieties. The varieties with a subunit report have been noted. Among them are the Izvor variety, known as the drought-tolerant variety, as well as other Romanian varieties: Alex, Delabrad, Lovrin 34, etc. An increased activity of catalase was present in most varieties, so there is the possibility of drought tolerance. Among the varieties highlighted are Romanian varieties (Dropia, Trivale, Nikifor, etc.) but also foreign varieties (Kristina, GH Hattyu, Karlygash, etc.). However, the correlation between yield index in the limited assortment and the antioxidant enzyme content ratios between PEG and control treatments does not exist, suggesting that none of these biochemical indicators are a selective indicator for drought tolerance under the experimental condition.

This cytogenetic study evaluates the biostimulation potential of the aqueous extract of seabuckthorn fruits (AESF) in plant cells, using the Allium cepa species as a test plant. The effects were monitored both at the macroscopic and microscopically level. The onion bulbs were exposed to the action of different concentrations of AESF (0.5, 1, 1.5, 2, and 2.5%) for 72 h. The obtained results showed the positive effect induced by the aqueous extract on the growth of the meristematic roots, but only at concentrations ranging between 0.5–1.5%, when the average length of the roots had values between 2.51–3.40 cm, which means an increase compared to the untreated control with 3.71–40.49%. Within the same concentration range of the AESF, an effect of intensifying the mitotic activity was recorded. On the other hand, at the 2–2.5% concentration of the AESF, there was an inhibitory effect on the growth of meristematic roots. Additionally, concentrations ≥2% of AESF induced a cytotoxic and genotoxic effect through the occurrence of some chromosomal and nuclear abnormalities in A. cepa cells (sticky, laggards, ring chromosomes, and micronucleus). The obtained results suggest the biostimulation potential of the AESF for plant cells and the possibility of using it as an eco-friendly fertilizer.

Urea is one of the most commonly used nitrogen fertilizers in Romania. The cytogenetic objectives of this experience were the evaluation of the effects of cytotoxicity and genotoxicity induced by the action of urea applied in different concentrations (100, 500, 1000, 5000, 10,000 and 20,000 ppm) for 72 h on the meristematic tissues of Allium cepa L., a species very commonly used in cytotoxicity tests. The statistical analysis of the obtained results indicates that with the increase in urea concentration, mitotic activity is inhibited, while the chromosomal aberration rate in the cells in mitosis as well as the frequency of nuclear abnormalities in the interphase cells increases. From this point of view, the main chromosomal aberrations identified were stickiness, laggards and C-mitosis, while nuclear abnormalities were the appearance of a large number of binuclear and multinucleated cells, some with ghost nuclei. In addition, at concentrations of over 1000 ppm and especially at 10,000 and 20,000 ppm urea the presence of cells with one or more micronuclei (even with nine micronuclei) was signalled, indicating a strong clastogenic potential of urea. The results suggest caution in the use of urea, by applying small concentrations, within the environmental limits, for the protection of the environment, but also of humans and animals. The concentrations we recommend to reduce the risk of pollution are between 100 and 500 ppm urea. However, further investigation is needed to confirm these findings.