The major goal of this study is to create a venue for further work on the effect of pulsed magnetic fields on plant metabolism. It deals with metabolite synthesis in the aforementioned conditions in microplants of Pyrus communis L. So far, there have been glimpses into the governing factors of plant biochemistry in vivo, and low-frequency pulsed magnestatic fields have been shown to induce additional electric currents in plant tissues, thus perturbing the value of cell membrane potential and causing the biosynthesis of new metabolites. In this study, sixty-seven metabolites synthesized in microplants within 3–72 h after treatment were identified and annotated. In total, thirty-one metabolites were produced. Magnetic-pulse treatment caused an 8.75-fold increase in the concentration of chlorogenic acid (RT = 8.33 ± 0.01₉₇ min) in tissues and the perturbation of phenolic composition. Aucubin, which has antiviral and antistress biological activity, was identified as well. This study sheds light on the effect of magnetic fields on the biochemistry of low-molecular-weight metabolites of pear plants in vitro, thus providing in-depth metabolite analysis under optimized synthetic conditions. This study utilized high-resolution gas chromatography-mass spectrometry, metabolomics methods, stochastic dynamics mass spectrometry, quantum chemistry, and chemometrics, respectively. Stochastic dynamics uses the relationships between measurands and molecular structures of silylated carbohydrates, showing virtually identical mass spectra and comparable chemometrics parameters.

Минеральный состав плодов ремонтантной малины ( Rubus idaeus L.)

The questions of the practical usage of the analytical scanning electron microscope JSM 600 LA by JEOL company (Japan) with EDS system – microanalysis were envisaged. This device was used to study the structure and elemental composition of the fruit ash received from 6 breeds of 3 plum species. Plum fruit are rich in mineral substances, the following order of substances accumulation is characteristic for them: K > P > Ca > Mg > Na ≈ S > Fe > Zn ≈ Cu > Mn ≈ Co > N i≈ Pb. In fruit pulp K is accumulated on 7 – 20 % more than in skin. The concentration of Ca, Cu, Fe, Ni, Zn and Pb in fruit skin is in 2 – 10 times higher than in pulp. The most of mineral substances is accumulated in the fruit of Prunus salicina L. plum specie. The significant correlations are determined between Ca and Mg (r 0.75) and Ca and Ni (r 0.71); the medium correlation is indicated between Ca and Cu (r 0.62) and Cu and Ni (r 0.55).

A comparative analysis of the seeds ash composition of the breed Rif (Brassica napus L.) rapeseeds grown in the Lipetskaya region was held. The plants were grown in the conditions of the agroecological experiment using mineral (NPK and zeolite) and organic (hen droppings) fertilizers. 6 variants of the experiment were studied – the plants are grown without fertilizers application (the control); the mineral fertilizer (N60:P60:K60) separately and together with zeolite (5 t.ha-1); the zeolite in pure form (5 t.ha-1); hen droppings (5 t.ha-1) separately and together with zeolite (5 t.ha-1). We studied the accumulation of 9 basic elements (in mass %) contained in Brassica napus. seeds ash using the method of energy-dispersive X-ray spectroscopy. The accumulation order of the elements was determined: P ≈ K > Mg ≥ Ca > Mo > S > Zn > Mn > Fe. The proportion of P fluctuated from 10.852 to 11.855 mass %; the proportion of K – from 9.933 till 12.343 mass %. The rapeseeds contained Mg, Ca, and Mo in similar concentrations within the range of 4.0 -5.8 mass %. The combined application of zeolite with organic fertilizer ensured the accumulation of the minerals in the seeds. Correlations between the elements were established. High correlation between elements K and Mo was found (r = 0.96); P and Mg (0.86); P and Fe (r = 0.94); C and Mo (r = 0.86). The positive effect of the combined organic-mineral fertilizers with poultry farms wastes usage on the mineral elements accumulation in rapeseeds was stated. It is noted that the accumulation of P, Ca, Mo, and S in rape seeds leads to a decrease in Zn.

The results of the field observations and biochemical researches of plum (PrunusrossicaErem.) fruits grown in the garden of intensive type with agrogenic soil in row spacing are presented in the article. The fruits of plum breeds Kubanskaya kometa и Naidena were gathered from the trees grafted on different stocks. The influene of the stock on the trees productivity, the fruit weight and biochemical structure of the plum fruits were studied. Basic chemical analyses, ascorbic acid concentration, antioxidant activity and fruit ash content were determined. The variation limits of the parameters under study depending on the used stock were shown. The best degustation evaluation parameters were found at the combinations of Kubanskaya kometa on stocks OPA-15-2 and Novinka, Naidena on OPA-15-2 (more than 4,5 points). The high positive correlation (r= 0.92) was marked between solutable solids and sugars content.The researches about the stock influence on the plum fruits titratable acidity change we have not found.The correlation between AA and ascorbic acid content in the fruits was determined (r =0.7). The decreasing row of the elements content in the plum fruits ash is the following: K>P>Mg>Ca>Mo>Cu>S>Ni>Fe≈Si>Mn. The proportion of K is from 18.67 (Naidena/ОРА-15-2) to 27.48 (Kubanskaya kometa/Novinka); P from 3.35 (Kubanskaya kometa/seedlings P.cerasifera Ehrh. to 6.39 (Kubanskaya kometa/Novinka), Mg from 1.11 (Nnaidena/ОРА-15-2) to 2.43 (Naidena/seedlings P.cerasifera Ehrh.) and Ca from 0.39 (Naidena/13-11) to 1.85 (Naidena/OPA-15-2) mass % respectively. The proportion of the rest elements is not more than 1 mass %. Under the OPA-15-2 stock influence the trees productivity and the single fruit weight increased, solutable solids, sugars and ascorbic acid concentration in the fruit in comparison with standard stock by the seedling rootstock of alycha (PrunuscerasiferaEhrh. seedlings). While ingrafting by Novinka stocks the parameters under study decreased.

Artemisinin-based drugs are the most effective medicine against multidrug-resistant Plasmodium spp., the parasite that causes malaria. To this day, wormwood A. annua L. is the sole commercial source of artemisinin, where it is produced in minor amounts. The artemisinin yield depends on numerous poorly regulated agricultural factors and the genetic variability of this non-domesticated plant. This has aroused significant interest in the development of heterologous expression platforms for artemisinin production. Previously, we obtained lines of Chrysanthemum morifolium Ramat. (C. morifolium Ramat.), cvs. White Snowdon and Egyptianka, transformed with artemisinin biosynthesis genes. Here, we report the results of an analysis of artemisinin production in transgenic chrysanthemums. Transcription of heterologous amorpha-4,11-diene monooxygenase and cytochrome P450 reductase genes in transgenic lines was confirmed using high-resolution melting analysis. Artemisinin accumulation was detected using GC-MS in White Snowdon plants, but not in Egyptianka ones, thereby demonstrating the possibility of transplanting active artemisinin biosynthetic pathway into chrysanthemum. Ways of increasing its content in producer plants are discussed.