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Jae-Heung Jeon
Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, Korea

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Journal article
Published: 16 April 2021 in Plants
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Both obtaining high-yielding, viable protoplasts and following reliable regeneration protocols are prerequisites for the continuous expansion and development of newly emerging systems involving protoplast utilization. This study determines an efficient process from protoplast isolation to shoot regeneration in vitro. The maximum yield of protoplast extraction, which was 6.36 ± 0.51 × 106 protoplasts/g fresh weight (FW), was approximately 3.7 times higher than that previously reported for potato protoplasts. To obtain data, wounded leaves were used by partially cutting both sides of the midrib, and isolated protoplasts were purified by the sucrose cushion method, with a sucrose concentration of 20%. We confirmed a significant effect on the extraction efficiency by measuring enzymolysis during a 6 h period, with three times more washing buffer than the amount normally used. Protoplasts fixed in alginate lenses with appropriate space were successfully recovered and developed into microcalli 2 weeks after culture. In addition, to induce high efficiency regeneration from protoplasts, calli in which greening occurred for 6 weeks were induced to develop shoots in regeneration medium solidified by Gelrite, and they presented a high regeneration efficiency of 86.24 ± 11.76%.

ACS Style

Ki-Beom Moon; Ji-Sun Park; Su-Jin Park; Hyo-Jun Lee; Hye-Sun Cho; Sung-Ran Min; Youn-Il Park; Jae-Heung Jeon; Hyun-Soon Kim. A More Accessible, Time-Saving, and Efficient Method for In Vitro Plant Regeneration from Potato Protoplasts. Plants 2021, 10, 781 .

AMA Style

Ki-Beom Moon, Ji-Sun Park, Su-Jin Park, Hyo-Jun Lee, Hye-Sun Cho, Sung-Ran Min, Youn-Il Park, Jae-Heung Jeon, Hyun-Soon Kim. A More Accessible, Time-Saving, and Efficient Method for In Vitro Plant Regeneration from Potato Protoplasts. Plants. 2021; 10 (4):781.

Chicago/Turabian Style

Ki-Beom Moon; Ji-Sun Park; Su-Jin Park; Hyo-Jun Lee; Hye-Sun Cho; Sung-Ran Min; Youn-Il Park; Jae-Heung Jeon; Hyun-Soon Kim. 2021. "A More Accessible, Time-Saving, and Efficient Method for In Vitro Plant Regeneration from Potato Protoplasts." Plants 10, no. 4: 781.

Journal article
Published: 28 May 2020 in Plants
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Flavonoids, including maackiain (Maac) from Sophora flavescens Aiton roots, have many pharmacological properties, such as antitumor, antimicrobial, and antifungal activities. This research aimed to develop an in vitro plant and callus culture system for S. flavescens for the purpose of generating an alternative production system for enhancing Maac production, as Maac is usually present in very small amounts in S. flavescens’ roots. We arranged the optimal conditions of different tissues of S. flavescens and supplemented the medium with various plant growth regulators (PGRs). The highest induction and proliferation rates of callus was shown in combination treatments of all concentrations of thidiazuron (TDZ) and picloram. In addition, calli induced with leaf explants cultured on 2.0 mg/L picloram and 0.5 mg/L 6-benzyladenine (BA) in Murashige and Skoog (MS) medium had the highest accumulation of the active metabolite Maac. In vitro shoots were regenerated on medium containing combinations of TDZ and α-Naphthalene acetic acid (NAA). A reliable protocol for the mass production of secondary metabolites using a callus culture of S. flavescens was successfully established.

ACS Style

Ji-Sun Park; Zuh-Kyung Seong; Mi-Sun Kim; Jang-Ho Ha; Ki-Beom Moon; Hyo-Jun Lee; Hyeong-Kyu Lee; Jae-Heung Jeon; Sang Un Park; Hyun-Soon Kim. Production of Flavonoids in Callus Cultures of Sophora flavescens Aiton. Plants 2020, 9, 688 .

AMA Style

Ji-Sun Park, Zuh-Kyung Seong, Mi-Sun Kim, Jang-Ho Ha, Ki-Beom Moon, Hyo-Jun Lee, Hyeong-Kyu Lee, Jae-Heung Jeon, Sang Un Park, Hyun-Soon Kim. Production of Flavonoids in Callus Cultures of Sophora flavescens Aiton. Plants. 2020; 9 (6):688.

Chicago/Turabian Style

Ji-Sun Park; Zuh-Kyung Seong; Mi-Sun Kim; Jang-Ho Ha; Ki-Beom Moon; Hyo-Jun Lee; Hyeong-Kyu Lee; Jae-Heung Jeon; Sang Un Park; Hyun-Soon Kim. 2020. "Production of Flavonoids in Callus Cultures of Sophora flavescens Aiton." Plants 9, no. 6: 688.

Review
Published: 24 December 2019 in Plants
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Over the last several decades, plants have been developed as a platform for the production of useful recombinant proteins due to a number of advantages, including rapid production and scalability, the ability to produce unique glycoforms, and the intrinsic safety of food crops. The expression methods used to produce target proteins are divided into stable and transient systems depending on applications that use whole plants or minimally processed forms. In the early stages of research, stable expression systems were mostly used; however, in recent years, transient expression systems have been preferred. The production of the plant itself, which produces recombinant proteins, is currently divided into two major approaches, open-field cultivation and closed-indoor systems. The latter encompasses such regimes as greenhouses, vertical farming units, cell bioreactors, and hydroponic systems. Various aspects of each system will be discussed in this review, which focuses mainly on practical examples and commercially feasible approaches.

ACS Style

Ki-Beom Moon; Ji-Sun Park; Youn-Il Park; In-Ja Song; Hyo-Jun Lee; Hye Sun Cho; Jae-Heung Jeon; Hyun-Soon Kim; Park. Development of Systems for the Production of Plant-Derived Biopharmaceuticals. Plants 2019, 9, 30 .

AMA Style

Ki-Beom Moon, Ji-Sun Park, Youn-Il Park, In-Ja Song, Hyo-Jun Lee, Hye Sun Cho, Jae-Heung Jeon, Hyun-Soon Kim, Park. Development of Systems for the Production of Plant-Derived Biopharmaceuticals. Plants. 2019; 9 (1):30.

Chicago/Turabian Style

Ki-Beom Moon; Ji-Sun Park; Youn-Il Park; In-Ja Song; Hyo-Jun Lee; Hye Sun Cho; Jae-Heung Jeon; Hyun-Soon Kim; Park. 2019. "Development of Systems for the Production of Plant-Derived Biopharmaceuticals." Plants 9, no. 1: 30.

Review
Published: 13 October 2016 in International Journal of Molecular Sciences
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Disease prevention through vaccination is considered to be the greatest contribution to public health over the past century. Every year more than 100 million children are vaccinated with the standard World Health Organization (WHO)-recommended vaccines including hepatitis B (HepB). HepB is the most serious type of liver infection caused by the hepatitis B virus (HBV), however, it can be prevented by currently available recombinant vaccine, which has an excellent record of safety and effectiveness. To date, recombinant vaccines are produced in many systems of bacteria, yeast, insect, and mammalian and plant cells. Among these platforms, the use of plant cells has received considerable attention in terms of intrinsic safety, scalability, and appropriate modification of target proteins. Research groups worldwide have attempted to develop more efficacious plant-derived vaccines for over 30 diseases, most frequently HepB and influenza. More inspiring, approximately 12 plant-made antigens have already been tested in clinical trials, with successful outcomes. In this study, the latest information from the last 10 years on plant-derived antigens, especially hepatitis B surface antigen, approaches are reviewed and breakthroughs regarding the weak points are also discussed.

ACS Style

Young Hee Joung; Se Hee Park; Ki-Beom Moon; Jae-Heung Jeon; Chang Won Choi; Hyun-Soon Kim. The Last Ten Years of Advancements in Plant-Derived Recombinant Vaccines against Hepatitis B. International Journal of Molecular Sciences 2016, 17, 1715 .

AMA Style

Young Hee Joung, Se Hee Park, Ki-Beom Moon, Jae-Heung Jeon, Chang Won Choi, Hyun-Soon Kim. The Last Ten Years of Advancements in Plant-Derived Recombinant Vaccines against Hepatitis B. International Journal of Molecular Sciences. 2016; 17 (10):1715.

Chicago/Turabian Style

Young Hee Joung; Se Hee Park; Ki-Beom Moon; Jae-Heung Jeon; Chang Won Choi; Hyun-Soon Kim. 2016. "The Last Ten Years of Advancements in Plant-Derived Recombinant Vaccines against Hepatitis B." International Journal of Molecular Sciences 17, no. 10: 1715.

Journal article
Published: 09 October 2014 in Plant Cell, Tissue and Organ Culture (PCTOC)
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Tissue-specific promoters for efficient expression of transgenes at specific times or in specific plant tissues can be applied to develop new transgenic plants. To exploit a promoter capable of driving strong expression in potato tubers, we isolated the promoter region of the laccase gene from potato (Solanum tuberosum L. cv. Desiree) and characterized its activity in transgenic Solanaceae plants, such as potato, tobacco and tomato. The ability of the laccase promoter to induce the β-glucuronidase (GUS) reporter was evaluated in independent transgenic potato lines and compared with that of the constitutive CaMV35S promoter. To determine the tissue specificity of expression in transgenic potato, GUS levels in shoot tips, leaves, stems, roots and tubers were measured by histochemical analysis. The laccase promoter conferred tuber-specific expression in transgenic potato regardless of the developmental stage, and there was no GUS reporter expression in leaves, stems or roots. Serial 5′ deletion analysis of the laccase promoter revealed that the tuber-specific regulatory elements might be scattered throughout the promoter. The laccase promoter responded weakly to salt stress, mannitol stress, and mechanical wounding but not to cold stress in the leaves and stems of transgenic potato. In transgenic tobacco, weak GUS expression driven by the laccase promoter was detected throughout the entire plant, whereas in transgenic tomato, GUS expression was detected only in the roots and seeds. Our data show that the laccase promoter represents a feasible candidate to drive high and preferential expression of genes in potato tubers.

ACS Style

Jang-Ho Ha; Ki-Beom Moon; Mi-Sun Kim; Se-Won Park; Kyu-Woong Hahn; Jae-Heung Jeon; Hyun-Soon Kim. The laccase promoter of potato confers strong tuber-specific expression in transgenic plants. Plant Cell, Tissue and Organ Culture (PCTOC) 2014, 120, 57 -68.

AMA Style

Jang-Ho Ha, Ki-Beom Moon, Mi-Sun Kim, Se-Won Park, Kyu-Woong Hahn, Jae-Heung Jeon, Hyun-Soon Kim. The laccase promoter of potato confers strong tuber-specific expression in transgenic plants. Plant Cell, Tissue and Organ Culture (PCTOC). 2014; 120 (1):57-68.

Chicago/Turabian Style

Jang-Ho Ha; Ki-Beom Moon; Mi-Sun Kim; Se-Won Park; Kyu-Woong Hahn; Jae-Heung Jeon; Hyun-Soon Kim. 2014. "The laccase promoter of potato confers strong tuber-specific expression in transgenic plants." Plant Cell, Tissue and Organ Culture (PCTOC) 120, no. 1: 57-68.

Journal article
Published: 01 December 2009 in Biotechnology and Bioprocess Engineering
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Comprehensive metabolite fingerprinting of transgenic potatoes that constitutively express human beta amyloid, curdlan synthase (CRDS), and glycogen synthase (glgA); and of wild-type potatoes was carried out using FT-IR and 1H NMR spectroscopy in combination with multivariate analyses. Comparison of metabolic patterns between transgenic and wild-type potatoes revealed that there were neither quantitative nor qualitative differences in metabolites between transgenic potatoes expressing human beta amyloid, CRDS or glgA, and non-transformed control potatoes. However, there were metabolic differences between two control potato lines — one that was fresh and the other stored. After 1 week of storage, comprehensive metabolite patterns were significantly modified. Although the differences between CRDS and glgA transgenic and control potato lines were small, PCA analysis of FT-IR and 1H NMR spectral data identified two distinct control lines. These results suggest that the comprehensive metabolite changes in control potato lines, which occurred after 1 week of storage, were greater than the differences between CRDS and glgA transgenic and wild-type potato lines. Thus, the combination of FT-IR and 1H NMR spectral data and multivariate analysis was valuable for the detection of comprehensive differences in metabolic profiles between transgenic and non-transformed control plants, even though peak-signal overlap prevented assignment of pure compounds. The combination of FT-IR and 1H NMR spectral data and multivariate analysis is a simple and rapid method for evaluation of the metabolic equivalence of GM crops.

ACS Style

Hyun Soon Kim; Suk Weon Kim; Young Seok Park; Suk Youn Kwon; Jang Ryol Liu; Hyouk Joung; Jae Heung Jeon. Metabolic profiles of genetically modified potatoes using a combination of metabolite fingerprinting and multivariate analysis. Biotechnology and Bioprocess Engineering 2009, 14, 738 -747.

AMA Style

Hyun Soon Kim, Suk Weon Kim, Young Seok Park, Suk Youn Kwon, Jang Ryol Liu, Hyouk Joung, Jae Heung Jeon. Metabolic profiles of genetically modified potatoes using a combination of metabolite fingerprinting and multivariate analysis. Biotechnology and Bioprocess Engineering. 2009; 14 (6):738-747.

Chicago/Turabian Style

Hyun Soon Kim; Suk Weon Kim; Young Seok Park; Suk Youn Kwon; Jang Ryol Liu; Hyouk Joung; Jae Heung Jeon. 2009. "Metabolic profiles of genetically modified potatoes using a combination of metabolite fingerprinting and multivariate analysis." Biotechnology and Bioprocess Engineering 14, no. 6: 738-747.

Review article
Published: 05 November 2009 in Advances in Botanical Research
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In plants, reactive oxygen species (ROS) are continuously produced from aerobic metabolic processes such as the photosynthetic and respiratory reactions. The cellular accumulation of ROS, which are highly reactive, is highly cytotoxic. Therefore, all the aerobic organisms have been evolved to develop efficient ROS-scavenging mechanisms. In recent years, the role of ROS in the regulation of plant growth and development has been identified. Increased ROS production is functionally coupled to the effects of plant growth regulators. The specific ROS that are related to specific plant hormones may control plant growth and development. The recent discovery of a tip-high, Ca2+-interdependent, ROS gradient produced by NADPH oxidase and its close association with polarized growth will provide information on the dual role of ROS in plants, as both the toxic byproducts of aerobic metabolism and the key regulators of growth and developmental pathways.

ACS Style

Hyun-Soon Kim; Yoon-Sik Kim; Kyu-Woong Hahn; Hyouk Joung; Jae-Heung Jeon. Chapter 2 Reactive Oxygen Species. Advances in Botanical Research 2009, 52, 25 -46.

AMA Style

Hyun-Soon Kim, Yoon-Sik Kim, Kyu-Woong Hahn, Hyouk Joung, Jae-Heung Jeon. Chapter 2 Reactive Oxygen Species. Advances in Botanical Research. 2009; 52 ():25-46.

Chicago/Turabian Style

Hyun-Soon Kim; Yoon-Sik Kim; Kyu-Woong Hahn; Hyouk Joung; Jae-Heung Jeon. 2009. "Chapter 2 Reactive Oxygen Species." Advances in Botanical Research 52, no. : 25-46.

Journal article
Published: 14 February 2008 in Plant Cell Reports
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Transgenic potato plants (SS2 and SS4) that overexpressed a chloroplastic copper/zinc superoxide dismutase lily gene were utilized as an H(2)O(2)-inducible system in order to study the role of H(2)O(2) as a signaling molecule in the biosynthesis of ethylene. SS2 and SS4 plants grown in vitro under sealed microenvironment (SME) conditions displayed anomalous phenotypes including reduction of stem elongation, radial stem growth, and promotion of root hair formation in the generated root, which were similar to ethylene-induced responses. In addition, SS4 plants showed severe vitrification in developing leaves and elevated ethylene production under SME conditions. After the ethylene action inhibitor AgNO(3), 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase (ACO) inhibitor CoCl(2), and ACC synthase inhibitor L -aminoethoxyvinylglycine were added to the growth media, the anomalous phenotypes in SS4 plants reverted to their normal phenotype with a concurrent decrease in ethylene production. Northern blot analysis showed that ACO transcripts in SS4 plants were constantly at high levels under normal and SME conditions, indicating that a high level of H(2)O(2) in SS4 plants up-regulates ACO transcripts. Moreover, the direct treatment of H(2)O(2) in potato plants confirmed the elevated expression of the ACO gene. Taken together, these data suggest that the high concentration of H(2)O(2) in transgenic potato plants stimulates ethylene biosynthesis by activating ACO gene expression.

ACS Style

Yoon-Sik Kim; Hyun-Soon Kim; Yong-Hwa Lee; Mi-Sun Kim; Hyun-Woo Oh; Kyu-Woong Hahn; Hyouk Joung; Jae-Heung Jeon. Elevated H2O2 production via overexpression of a chloroplastic Cu/ZnSOD gene of lily (Lilium oriental hybrid ‘Marco Polo’) triggers ethylene synthesis in transgenic potato. Plant Cell Reports 2008, 27, 973 -983.

AMA Style

Yoon-Sik Kim, Hyun-Soon Kim, Yong-Hwa Lee, Mi-Sun Kim, Hyun-Woo Oh, Kyu-Woong Hahn, Hyouk Joung, Jae-Heung Jeon. Elevated H2O2 production via overexpression of a chloroplastic Cu/ZnSOD gene of lily (Lilium oriental hybrid ‘Marco Polo’) triggers ethylene synthesis in transgenic potato. Plant Cell Reports. 2008; 27 (6):973-983.

Chicago/Turabian Style

Yoon-Sik Kim; Hyun-Soon Kim; Yong-Hwa Lee; Mi-Sun Kim; Hyun-Woo Oh; Kyu-Woong Hahn; Hyouk Joung; Jae-Heung Jeon. 2008. "Elevated H2O2 production via overexpression of a chloroplastic Cu/ZnSOD gene of lily (Lilium oriental hybrid ‘Marco Polo’) triggers ethylene synthesis in transgenic potato." Plant Cell Reports 27, no. 6: 973-983.

Journal article
Published: 01 January 2008 in BMC Biotechnology
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Patatins encoded by a multi-gene family are one of the major storage glycoproteins in potato tubers. Potato tubers have recently emerged as bioreactors for the production of human therapeutic glycoproteins (vaccines). Increasing the yield of recombinant proteins, targeting the produced proteins to specific cellular compartments, and diminishing expensive protein purification steps are important research goals in plant biotechnology. In the present study, potato patatins were eliminated almost completely via RNA interference (RNAi) technology to develop potato tubers as a more efficient protein expression system. The gene silencing effect of patatins in the transgenic potato plants was examined at individual isoform levels.

ACS Style

Yoon-Sik Kim; Yong-Hwa Lee; Hyun-Soon Kim; Mi-Sun Kim; Kyu-Woong Hahn; Jeong-Heon Ko; Hyouk Joung; Jae-Heung Jeon. Development of patatin knockdown potato tubers using RNA interference (RNAi) technology, for the production of human-therapeutic glycoproteins. BMC Biotechnology 2008, 8, 36 -36.

AMA Style

Yoon-Sik Kim, Yong-Hwa Lee, Hyun-Soon Kim, Mi-Sun Kim, Kyu-Woong Hahn, Jeong-Heon Ko, Hyouk Joung, Jae-Heung Jeon. Development of patatin knockdown potato tubers using RNA interference (RNAi) technology, for the production of human-therapeutic glycoproteins. BMC Biotechnology. 2008; 8 (1):36-36.

Chicago/Turabian Style

Yoon-Sik Kim; Yong-Hwa Lee; Hyun-Soon Kim; Mi-Sun Kim; Kyu-Woong Hahn; Jeong-Heon Ko; Hyouk Joung; Jae-Heung Jeon. 2008. "Development of patatin knockdown potato tubers using RNA interference (RNAi) technology, for the production of human-therapeutic glycoproteins." BMC Biotechnology 8, no. 1: 36-36.

Journal article
Published: 01 December 2007 in Journal of Plant Biology
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To determine their tissue-specific expression, three GRX genes were isolated from ESTs of a “6-hr post-suberization” cDNA library forSolatium tuberosum L cv. Desiree. Transcripts of a CCxC/S type GRX, StGRX1, accumulated to higher levels during the vegetative-growth stage in the leaves, stem, and roots. In contrast, transcripts of a CxxC/S type GRX, StGRX2, accumulated in the reproductive tissues, such as the flower and tuber. Differential expression of these three potato StGRXs suggest specific and unique physiological roles during plant development and in response to environmental cues. Therefore, we propose that StGRX1 is a housekeeping gene, functioning in the rapid defense against various abiotic and biotic stresses, while StGRX2 and-3 are selectively expressed during growth and in response to internal and external signals.

ACS Style

Won-Jin Kang; Hyun-Soon Kim; Youn-Il Park; Hyouk Joung; Jae-Heung Jeon. Differential Expression in Response to Biotic and Abiotic Stress from Three Potato Glutaredoxins Induced during Suberization. Journal of Plant Biology 2007, 50, 663 -670.

AMA Style

Won-Jin Kang, Hyun-Soon Kim, Youn-Il Park, Hyouk Joung, Jae-Heung Jeon. Differential Expression in Response to Biotic and Abiotic Stress from Three Potato Glutaredoxins Induced during Suberization. Journal of Plant Biology. 2007; 50 (6):663-670.

Chicago/Turabian Style

Won-Jin Kang; Hyun-Soon Kim; Youn-Il Park; Hyouk Joung; Jae-Heung Jeon. 2007. "Differential Expression in Response to Biotic and Abiotic Stress from Three Potato Glutaredoxins Induced during Suberization." Journal of Plant Biology 50, no. 6: 663-670.

Cell biology and morphogenesis
Published: 30 May 2007 in Plant Cell Reports
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A higher concentration of H2O2 was detected in the sense transgenic potato plant (SS4) with the lily chCu,ZnSOD sequence, whereas higher levels of O2(-) was detected in the antisense transgenic plant (SA1) than the WT plant. The elongation growth in SA1 was significantly inhibited by treatment with diphenyleneiodonium, an inhibitor of O2(-) generation, and promoted in the SS4 on treatment with herbicide methyl viologen, a generator of apoplastic O2(-) . Higher concentrations of GAs were detected during plant growth and the early stage of tuberization in SA1. Complete recovery of the above elongation growth and microtuberization pattern in transgenic plants following treatment of GA(3) or an inhibitor of gibberellin synthesis, paclobutrazol, indicate that these changes were mainly caused by active GA levels. In conclusion, a specific ROS (O2(-) ) acts as a signal transducer via GA biosynthetic pathways for the regulation of plant growth and tuber development of potato.

ACS Style

Mi-Sun Kim; Hyun-Soon Kim; Yoon-Shik Kim; Kwang-Hyun Baek; Hyun-Woo Oh; Kyu-Woong Hahn; Ro-Na Bae; In-Jung Lee; Hyouk Joung; Jae-Heung Jeon. Superoxide anion regulates plant growth and tuber development of potato. Plant Cell Reports 2007, 26, 1717 -1725.

AMA Style

Mi-Sun Kim, Hyun-Soon Kim, Yoon-Shik Kim, Kwang-Hyun Baek, Hyun-Woo Oh, Kyu-Woong Hahn, Ro-Na Bae, In-Jung Lee, Hyouk Joung, Jae-Heung Jeon. Superoxide anion regulates plant growth and tuber development of potato. Plant Cell Reports. 2007; 26 (10):1717-1725.

Chicago/Turabian Style

Mi-Sun Kim; Hyun-Soon Kim; Yoon-Shik Kim; Kwang-Hyun Baek; Hyun-Woo Oh; Kyu-Woong Hahn; Ro-Na Bae; In-Jung Lee; Hyouk Joung; Jae-Heung Jeon. 2007. "Superoxide anion regulates plant growth and tuber development of potato." Plant Cell Reports 26, no. 10: 1717-1725.

Journal article
Published: 11 March 2003 in Biochemical and Biophysical Research Communications
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We isolated the chalcone reductase (pl-chr) gene of Pueraria montana var. lobata by using a PCR strategy from cDNA pools of storage roots. A high level of expression of RNA was found in both stems and roots. The genomic Southern blot result suggests that pl-chr exists as a member of a small gene family. By introducing a pl-chr gene under the control of the 35S CaMV promoter into the pink-flowering Xanthi line of Nicotiana tabacum, the flower color was changed from pink to white-to-pink. The contents of anthocyanin in the flowers of the transgenic lines were dramatically decreased by 40%, but the total UV absorption compounds remained unchanged. The production of liquiritigenin in pl-chr overexpressed transgenic tobacco lines was confirmed by HPLC and MS analysis. The introduction of pl-chr gene provides a method to redirect the flavonoid pathway into 5'-deoxyflavonoid production in non-legume crops, in order to manipulate the phenylpropanoid pathway for isoflavonoid production.

ACS Style

Jae-Youl Joung; G Mangai Kasthuri; Ji-Young Park; Won-Jin Kang; Hyun-Soon Kim; Bong-Sik Yoon; Hyouk Joung; Jae-Heung Jeon. An overexpression of chalcone reductase of Pueraria montana var. lobata alters biosynthesis of anthocyanin and 5'-deoxyflavonoids in transgenic tobacco. Biochemical and Biophysical Research Communications 2003, 303, 326 -331.

AMA Style

Jae-Youl Joung, G Mangai Kasthuri, Ji-Young Park, Won-Jin Kang, Hyun-Soon Kim, Bong-Sik Yoon, Hyouk Joung, Jae-Heung Jeon. An overexpression of chalcone reductase of Pueraria montana var. lobata alters biosynthesis of anthocyanin and 5'-deoxyflavonoids in transgenic tobacco. Biochemical and Biophysical Research Communications. 2003; 303 (1):326-331.

Chicago/Turabian Style

Jae-Youl Joung; G Mangai Kasthuri; Ji-Young Park; Won-Jin Kang; Hyun-Soon Kim; Bong-Sik Yoon; Hyouk Joung; Jae-Heung Jeon. 2003. "An overexpression of chalcone reductase of Pueraria montana var. lobata alters biosynthesis of anthocyanin and 5'-deoxyflavonoids in transgenic tobacco." Biochemical and Biophysical Research Communications 303, no. 1: 326-331.