The demand for real-time monitoring of cell functions and cell conditions has dramatically increased with the emergence of organ-on-a-chip (OOC) systems. However, the incorporation of co-cultures and microfluidic channels in OOC systems increases their biological complexity and therefore makes the analysis and monitoring of analytical parameters inside the device more difficult. In this work, we present an approach to integrate multiple sensors in an extremely thin, porous and delicate membrane inside a liver-on-a-chip device. Specifically, three electrochemical dissolved oxygen (DO) sensors were inkjet-printed along the microfluidic channel allowing local online monitoring of oxygen concentrations. This approach demonstrates the existence of an oxygen gradient up to 17.5% for rat hepatocytes and 32.5% for human hepatocytes along the bottom channel. Such gradients are considered crucial for the appearance of zonation of the liver. Inkjet printing (IJP) was the selected technology as it allows drop on demand material deposition compatible with delicate substrates, as used in this study, which cannot withstand temperatures higher than 130 °C. For the deposition of uniform gold and silver conductive inks on the porous membrane, a primer layer using SU-8 dielectric material was used to seal the porosity of the membrane at defined areas, with the aim of building a uniform sensor device. As a proof-of-concept, experiments with cell cultures of primary human and rat hepatocytes were performed, and oxygen consumption rate was stimulated with carbonyl-cyanide-4-(trifluoromethoxy)phenylhydrazone (FCCP), accelerating the basal respiration of 0.23 ± 0.07 nmol s⁻¹/10⁶ cells up to 5.95 ± 0.67 nmol s⁻¹/10⁶ cells s for rat cells and the basal respiration of 0.17 ± 0.10 nmol s⁻¹/10⁶ cells by up to 10.62 ± 1.15 nmol s⁻¹/10⁶ cells for human cells, with higher oxygen consumption of the cells seeded at the outflow zone. These results demonstrate that the approach of printing sensors inside an OOC has tremendous potential because IJP is a feasible technique for the integration of different sensors for evaluating metabolic activity of cells, and overcomes one of the major challenges still remaining on how to tap the full potential of OOC systems.

We report on the detailed electrical investigation of all-inkjet-printed thin-film transistor (TFT) arrays focusing on TFT failures and their origins. The TFT arrays were manufactured on flexible polymer substrates in ambient condition without the need for cleanroom environment or inert atmosphere and at a maximum temperature of 150 °C. Alternative manufacturing processes for electronic devices such as inkjet printing suffer from lower accuracy compared to traditional microelectronic manufacturing methods. Furthermore, usually printing methods do not allow the manufacturing of electronic devices with high yield (high number of functional devices). In general, the manufacturing yield is much lower compared to the established conventional manufacturing methods based on lithography. Thus, the focus of this contribution is set on a comprehensive analysis of defective TFTs printed by inkjet technology. Based on root cause analysis, we present the defects by developing failure categories and discuss the reasons for the defects. This procedure identifies failure origins and allows the optimization of the manufacturing resulting finally to a yield improvement.

A high data capacity chipless radio frequency identification (chipless-RFID) system, useful for security and authentication applications, is presented in this paper. Reading is based on the near-field coupling between the tag, a chain of identical split-ring resonators (SRRs) printed on a (typically flexible) dielectric substrate (e.g., liquid crystal polymer, plastic, and paper), and the reader. Encoding is achieved by the presence or absence of SRRs at predefined (equidistant) positions in the chain, and tag identification (ID) is based on sequential bit reading. Namely, the tag must be longitudinally displaced, at short distance, over the reader, a microstrip line loaded with an SRR and fed by a harmonic signal. By this means, the harmonic signal is amplitude modulated, and the (ID) code is contained in the envelope function, which can be obtained by means of an envelope detector. With this system, tag reading requires proximity with the reader, but this is not an issue in many applications within the domain of security and authentication (e.g., secure paper for corporate documents and certificates). Several circularly shaped 40-bit encoders (implemented in a commercial microwave substrate), and the corresponding reader, are designed and fabricated as proof-of-concept demonstrators. Strategies for programming the tags and a first proof-of-concept chipless-RFID tag fabricated on plastic substrate through inkjet printing are included in this paper.

In this letter, a chipless radio frequency identification (chipless-RFID) system with erasable/programmable 40-bit tags inkjet printed on paper substrates, where tag reading proceeds sequentially through near-field coupling, is presented for the first time. The tags consist of a linear chain of identical split ring resonators (SRRs) printed at predefined and equidistant positions on a paper substrate, and each resonant element provides a bit of information. Tag programming is achieved by cutting certain resonant elements, providing the logic state “0” to the corresponding bit. Conversely, tags can be erased (all bits set to “1”) by short circuiting those previously cut resonant elements through inkjet. An important feature of the proposed system is the fact that tag reading is possible either with the SRR chain faced up or faced down (with regard to the reader). To this end, two pairs of header bits (resonators), with different sequences, have been added at the beginning and at the end of the tag identification chain. Moreover, tag data storage capacity (number of bits) is only limited by the space occupied by the linear chain. The implementation of tags on paper substrates demonstrates the potential of the proposed chipless-RFID system in secure paper applications, where the necessary proximity between the reader and the tag, inherent to near-field reading, is not an issue.