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Cyanotoxins (CTs) produced by cyanobacteria in surface freshwater are a major threat for public health and aquatic ecosystems. Cyanobacteria can also produce a wide variety of other understudied bioactive metabolites such as oligopeptides microginins (MGs), aeruginosins (AERs), aeruginosamides (AEGs) and anabaenopeptins (APs). This study reports on the co-occurrence of CTs and cyanopeptides (CPs) in Lake Vegoritis, Greece and presents their variant-specific profiles obtained during 3-years of monitoring (2018–2020). Fifteen CTs (cylindrospermopsin (CYN), anatoxin (ATX), nodularin (NOD), and 12 microcystins (MCs)) and ten CPs (3 APs, 4 MGs, 2 AERs and aeruginosamide (AEG A)) were targeted using an extended and validated LC-MS/MS protocol for the simultaneous determination of multi-class CTs and CPs. Results showed the presence of MCs (MC-LR, MC-RR, MC-YR, dmMC-LR, dmMC-RR, MC-HtyR, and MC-HilR) and CYN at concentrations of <1 μg/L, with MC-LR (79%) and CYN (71%) being the most frequently occurring. Anabaenopeptins B (AP B) and F (AP F) were detected in almost all samples and microginin T1 (MG T1) was the most abundant CP, reaching 47.0 μg/L. This is the first report of the co-occurrence of CTs and CPs in Lake Vegoritis, which is used for irrigation, fishing and recreational activities. The findings support the need for further investigations of the occurrence of CTs and the less studied cyanobacterial metabolites in lakes, to promote risk assessment with relevance to human exposure.
Sevasti-Kiriaki Zervou; Kimon Moschandreou; Aikaterina Paraskevopoulou; Christophoros Christophoridis; Elpida Grigoriadou; Triantafyllos Kaloudis; Theodoros Triantis; Vasiliki Tsiaoussi; Anastasia Hiskia. Cyanobacterial Toxins and Peptides in Lake Vegoritis, Greece. Toxins 2021, 13, 394 .
AMA StyleSevasti-Kiriaki Zervou, Kimon Moschandreou, Aikaterina Paraskevopoulou, Christophoros Christophoridis, Elpida Grigoriadou, Triantafyllos Kaloudis, Theodoros Triantis, Vasiliki Tsiaoussi, Anastasia Hiskia. Cyanobacterial Toxins and Peptides in Lake Vegoritis, Greece. Toxins. 2021; 13 (6):394.
Chicago/Turabian StyleSevasti-Kiriaki Zervou; Kimon Moschandreou; Aikaterina Paraskevopoulou; Christophoros Christophoridis; Elpida Grigoriadou; Triantafyllos Kaloudis; Theodoros Triantis; Vasiliki Tsiaoussi; Anastasia Hiskia. 2021. "Cyanobacterial Toxins and Peptides in Lake Vegoritis, Greece." Toxins 13, no. 6: 394.
Microginins are the less common class of bioactive linear cyanobacterial peptides. Recently, an investigation for their presence in cyanobacteria from Greek freshwaters and strain cultures was carried out. The present dataset is related to the research article “New microginins from cyanobacteria of Greek freshwaters” [1]. Cyanobacterial biomass from bloom samples and cultured strains were extracted with aqueous methanol. Extracts were analysed by liquid chromatography coupled to hybrid triple quadrupole/linear ion trap mass spectrometer (LC-qTRAP MS/MS) in information dependent acquisition (IDA) mode. Enhanced ion product (EIP) mode was applied for the collection of ion fragmentation spectra. Identification of microginins was based on the characteristic fragment ions of the unique microginin amino acid 3-amino-2-hydroxy-decanoic acid (Ahda) and its modified forms. The analysis of fragmentation spectra revealed 51 microginin structures, including 36 new variants. This article provides the dataset of fragmentation mass spectra of the microginins detected in cyanobacteria from Greek freshwaters. As this class of cyanopeptides is produced by cyanobacteria from different geographical regions, the aim of this dataset is to enable the identification of microginins in future studies and therefore to contribute to a better evaluation of their presence in freshwater bodies worldwide.
Sevasti – Kiriaki Zervou; Triantafyllos Kaloudis; Anastasia Hiskia; Hanna Mazur-Marzec. Fragmentation mass spectra dataset of linear cyanopeptides - microginins. Data in Brief 2020, 31, 105825 .
AMA StyleSevasti – Kiriaki Zervou, Triantafyllos Kaloudis, Anastasia Hiskia, Hanna Mazur-Marzec. Fragmentation mass spectra dataset of linear cyanopeptides - microginins. Data in Brief. 2020; 31 ():105825.
Chicago/Turabian StyleSevasti – Kiriaki Zervou; Triantafyllos Kaloudis; Anastasia Hiskia; Hanna Mazur-Marzec. 2020. "Fragmentation mass spectra dataset of linear cyanopeptides - microginins." Data in Brief 31, no. : 105825.
Marine cyanobacteria are considered a prolific source of bioactive natural products with a range of biotechnological and pharmacological applications. However, data on the production of natural compounds from sponge-associated cyanobacteria are scarce. This study aimed to assess the potential of sponge-associated cyanobacteria strains representing different taxonomic groups for the production of bioactive compounds and the biological activity of their extracts. Phylogenetic analysis of sponge-associated cyanobacteria and screening for the presence of genes encoding non-ribosomal peptide synthetases (NRPSs) and polyketide synthases (PKSs) were performed. Methanol extracts of the sponge-associated strains were analyzed for cyanotoxin production and tested for antioxidant activity and cytotoxic activity against several human cancer cell lines and pathogenic bacteria. PKS were detected in all sponge-associated strains examined, indicating the metabolic potential of the isolates. PKS genes were more ubiquitous than NRPS genes. Cyanotoxins (i.e., cylindrospermopsin, anatoxin-a, nodularin, and microcystins) were not detected in any of the sponge-associated cyanobacterial strains. Strains belonging to Leptothoe, Pseudanabaena, and Synechococcus were found to have activity mainly against Staphylococcus aureus. In addition, sponge-associated Leptothoe strains (TAU-MAC 0915, 1015, 1115, and 1215) were found to be highly cytotoxic and in most cases more effective against human cancer cell lines than against normal cells. Extracts with the most promising bioactivity deserve further investigation in order to isolate and identify the bioactive molecule(s).
Despoina Konstantinou; Eleni Mavrogonatou; Sevasti-Kiriaki Zervou; Panagiotis Giannogonas; Spyros Gkelis. Bioprospecting Sponge-Associated Marine Cyanobacteria to Produce Bioactive Compounds. Toxins 2020, 12, 73 .
AMA StyleDespoina Konstantinou, Eleni Mavrogonatou, Sevasti-Kiriaki Zervou, Panagiotis Giannogonas, Spyros Gkelis. Bioprospecting Sponge-Associated Marine Cyanobacteria to Produce Bioactive Compounds. Toxins. 2020; 12 (2):73.
Chicago/Turabian StyleDespoina Konstantinou; Eleni Mavrogonatou; Sevasti-Kiriaki Zervou; Panagiotis Giannogonas; Spyros Gkelis. 2020. "Bioprospecting Sponge-Associated Marine Cyanobacteria to Produce Bioactive Compounds." Toxins 12, no. 2: 73.
Cyanobacteria can form extensive blooms in water with concurrent production and release of a large number of chemically diverse and bioactive metabolites, including hazardous toxins. Significant number of the metabolites belongs to non-ribosomal peptides, with unique residues, unusual structures and great potential for biotechnological application. The biosynthetic pathways of the peptides generate tens of variants, but only part of them has been identified. Microginins are an understudied class of cyanobacterial linear peptides with a characteristic decanoic acid derivative amino acid residue in their structure. In this study, cyanobacterial blooms and isolated strains from Greek lakes were analyzed for the presence of microginins by liquid chromatography coupled to hybrid triple quadrupole/linear ion trap mass spectrometer (LC-qTRAP MS/MS). Microginin structures were elucidated based on the obtained fragmentation spectra. A large number of microginins occurred in blooms of Greek freshwaters and the most frequently detected were Microginin FR1 (70% of samples), Microginin T1 (52%), Microginin 565B (52%), Microginin T2 (43%), and Microginin 565A (43%). Additionally, nine cyanobacterial strains i.e. Nostoc oryzae, Synechococcus sp., Microcystis aeruginosa, Microcystis viridis, and five Microcystis sp., were found to produce microginins. Thirty-six new microginin structures were characterized out of fifty-one totally detected variants. This is the first time that such a diversity of microginins is reported to be present in water bodies. Results clearly demonstrate the great metabolomic potential of cyanobacteria that inhabit Greek freshwaters and significantly expand the knowledge of cyanobacterial secondary metabolites with regards to the class of microginins.
Sevasti - Kiriaki Zervou; Spyros Gkelis; Triantafyllos Kaloudis; Anastasia Hiskia; Hanna Mazur-Marzec. New microginins from cyanobacteria of Greek freshwaters. Chemosphere 2020, 248, 125961 .
AMA StyleSevasti - Kiriaki Zervou, Spyros Gkelis, Triantafyllos Kaloudis, Anastasia Hiskia, Hanna Mazur-Marzec. New microginins from cyanobacteria of Greek freshwaters. Chemosphere. 2020; 248 ():125961.
Chicago/Turabian StyleSevasti - Kiriaki Zervou; Spyros Gkelis; Triantafyllos Kaloudis; Anastasia Hiskia; Hanna Mazur-Marzec. 2020. "New microginins from cyanobacteria of Greek freshwaters." Chemosphere 248, no. : 125961.
Cyanobacteria are a diverse group of photosynthetic Gram-negative bacteria that produce an array of secondary compounds with selective bioactivity against a broad spectrum of organisms and cell lines. In this study, 29 strains isolated from freshwaters in Greece were classified using a polyphasic approach and assigned to Chroococcales, Synechococcales, and Nostocales, representing 11 genera and 17 taxa. There were good agreements between 16S ribosomal RNA (rRNA)–cpcBA–internal genetic spacer (IGS) characterization and morphological features, except for the Jaaginema–Limnothrix group which appears intermixed and needs further elucidation. Methanol extracts of the strains were analyzed for cyanotoxin production and tested against pathogenic bacteria species and several cancer cell lines. We report for the first time a Nostoc oryzae strain isolated from rice fields capable of producing microcystins (MCs) and a Chlorogloeopsis fritschii strain isolated from the plankton of a lake, suggesting that this species may also occur in freshwater temperate habitats. Strains with very high or identical 16S rRNA gene sequences displayed different antibacterial and cytotoxic activities. Extracts from Synechococcus cf. nidulans showed the most potent antibacterial activity against Staphylococcus aureus, whereas Jaaginema sp. strains exhibited potent cytotoxic activities against human colorectal adenocarcinoma and hepatocellular carcinoma cells. Jaaginema Thessaloniki Aristotle University Microalgae and Cyanobacteria (TAU-MAC) 0110 and 0210 strains caused pronounced changes in the actin network and triggered the formation of numerous lipid droplets in hepatocellular carcinoma and green monkey kidney cells, suggesting oxidative stress and/or mitochondrial damage leading to apoptosis.
Spyros Gkelis; Manthos Panou; Despoina Konstantinou; Panagiotis Apostolidis; Antonia Kasampali; Sofia Papadimitriou; Dominiki Kati; Giorgia Maria Di Lorenzo; Stamatia Ioakeim; Sevasti-Kiriaki Zervou; Christophoros Christophoridis; Theodoros M. Triantis; Triantafyllos Kaloudis; Anastasia Hiskia; Minas Arsenakis. Diversity, Cyanotoxin Production, and Bioactivities of Cyanobacteria Isolated from Freshwaters of Greece. Toxins 2019, 11, 436 .
AMA StyleSpyros Gkelis, Manthos Panou, Despoina Konstantinou, Panagiotis Apostolidis, Antonia Kasampali, Sofia Papadimitriou, Dominiki Kati, Giorgia Maria Di Lorenzo, Stamatia Ioakeim, Sevasti-Kiriaki Zervou, Christophoros Christophoridis, Theodoros M. Triantis, Triantafyllos Kaloudis, Anastasia Hiskia, Minas Arsenakis. Diversity, Cyanotoxin Production, and Bioactivities of Cyanobacteria Isolated from Freshwaters of Greece. Toxins. 2019; 11 (8):436.
Chicago/Turabian StyleSpyros Gkelis; Manthos Panou; Despoina Konstantinou; Panagiotis Apostolidis; Antonia Kasampali; Sofia Papadimitriou; Dominiki Kati; Giorgia Maria Di Lorenzo; Stamatia Ioakeim; Sevasti-Kiriaki Zervou; Christophoros Christophoridis; Theodoros M. Triantis; Triantafyllos Kaloudis; Anastasia Hiskia; Minas Arsenakis. 2019. "Diversity, Cyanotoxin Production, and Bioactivities of Cyanobacteria Isolated from Freshwaters of Greece." Toxins 11, no. 8: 436.
Increased rates of reactive oxygen/nitrogen species (ROS/RNS) are involved in almost all cancer types, associated with tumor development and progression, causing damage to biomolecules such as proteins, nucleic acids and membrane lipids, in different biological compartments. We used a human tumor xenograft mouse model to evaluate for the first time in parallel the remodeling of fatty acid moieties in erythrocyte membrane phospholipids and the level of ROS-induced DNA lesions in liver and kidney tissues. Using liquid chromatography tandem mass spectrometry the 5’R and 5’S diastereoisomers of 5’,8-cyclo-2’-deoxyadenosine and 5’,8-cyclo-2’-deoxyguanosine, together with 8-oxo-7,8-dihydro-2’-deoxyadenosine, were determined in mice at young (4- and 5-weeks) and old (17-weeks) ages and compared with control SCID mice without tumor implantation. Tumor-bearing mice showed a higher level of ROS-damaged nucleosides in genomic DNA as the age and tumor progress, compared to controls (1.07–1.53-fold in liver and 1.1–1.4-fold in kidney, respectively). The parallel fatty acid profile of erythrocyte membranes showed a profound lipid remodeling during tumor and age progression consisting of PUFA consumption and SFA enrichment (ca 28% and 58%, respectively, in late stage tumor-bearing mice), markers of enhanced oxidative and proliferative processes, respectively. Membrane lipid remodeling and ROS-induced DNA lesions may be combined to afford an integrated scenario of cancer progression and ageing, reinforcing a holistic vision among molecular markers rather than the biomarker identification in a single compartment.
Marios G. Krokidis; Maria Louka; Eleni K. Efthimiadou; Sevasti-Kiriaki Zervou; Kyriakos Papadopoulos; Anastasia Hiskia; Carla Ferreri; Chryssostomos Chatgilialoglu. Membrane Lipidome Reorganization and Accumulation of Tissue DNA Lesions in Tumor-Bearing Mice: An Exploratory Study. Cancers 2019, 11, 480 .
AMA StyleMarios G. Krokidis, Maria Louka, Eleni K. Efthimiadou, Sevasti-Kiriaki Zervou, Kyriakos Papadopoulos, Anastasia Hiskia, Carla Ferreri, Chryssostomos Chatgilialoglu. Membrane Lipidome Reorganization and Accumulation of Tissue DNA Lesions in Tumor-Bearing Mice: An Exploratory Study. Cancers. 2019; 11 (4):480.
Chicago/Turabian StyleMarios G. Krokidis; Maria Louka; Eleni K. Efthimiadou; Sevasti-Kiriaki Zervou; Kyriakos Papadopoulos; Anastasia Hiskia; Carla Ferreri; Chryssostomos Chatgilialoglu. 2019. "Membrane Lipidome Reorganization and Accumulation of Tissue DNA Lesions in Tumor-Bearing Mice: An Exploratory Study." Cancers 11, no. 4: 480.
Toxic cyanobacteria occur in Greek surface water bodies. However, studies on the occurrence of cyanotoxins (CTs) are often limited to mainly microcystins (MCs), with use of screening methods, such as ELISA, that are not conclusive of the chemical structure of the CT variants and can be subject to false positive results. A multi-lake survey in Greece (14 lakes) was conducted in water and biomass, targeted to a wide range of multi-class CTs including MCs, nodularin-R (NOD), cylindrospermopsin (CYN), anatoxin-a (ANA-a) and saxitoxins (STXs), using multi-class/variant LC-MS/MS analytical workflows, achieving sensitive detection, definitive identification and accurate quantitation. A wide variety of CTs (CYN, ANA-a, STX, neoSTX, dmMC-RR, MC-RR, MC-YR, MC-HtyR, dm3MC-LR, MC-LR, MC-HilR, MC-WR, MC-LA, MC-LY, MC-LW and MC-LF), were detected, with MCs being the most commonly occurring. In biomass, MC-RR was the most abundant toxin, reaching 754 ng mg−1 dw, followed by MC-LR (458 ng mg−1 dw). CYN and ANA-a were detected for the first time in the biomass of Greek lakes at low concentrations and STXs in lakes Trichonis, Vistonis and Petron. The abundance and diversity of CTs were also evaluated in relation to recreational health risks, in a case study with a proven history of MCs (Lake Kastoria).
Christophoros Christophoridis; Sevasti-Kiriaki Zervou; Korina Manolidi; Matina Katsiapi; Maria Moustaka-Gouni; Triantafyllos Kaloudis; Theodoros M. Triantis; Anastasia Hiskia. Occurrence and diversity of cyanotoxins in Greek lakes. Scientific Reports 2018, 8, 1 -22.
AMA StyleChristophoros Christophoridis, Sevasti-Kiriaki Zervou, Korina Manolidi, Matina Katsiapi, Maria Moustaka-Gouni, Triantafyllos Kaloudis, Theodoros M. Triantis, Anastasia Hiskia. Occurrence and diversity of cyanotoxins in Greek lakes. Scientific Reports. 2018; 8 (1):1-22.
Chicago/Turabian StyleChristophoros Christophoridis; Sevasti-Kiriaki Zervou; Korina Manolidi; Matina Katsiapi; Maria Moustaka-Gouni; Triantafyllos Kaloudis; Theodoros M. Triantis; Anastasia Hiskia. 2018. "Occurrence and diversity of cyanotoxins in Greek lakes." Scientific Reports 8, no. 1: 1-22.
Under ongoing climate change and increasing anthropogenic activity, which continuously challenge ecosystem resilience, an in-depth understanding of ecological processes is urgently needed. Lakes, as providers of numerous ecosystem services, face multiple stressors that threaten their functioning. Harmful cyanobacterial blooms are a persistent problem resulting from nutrient pollution and climate-change induced stressors, like poor transparency, increased water temperature and enhanced stratification. Consistency in data collection and analysis methods is necessary to achieve fully comparable datasets and for statistical validity, avoiding issues linked to disparate data sources. The European Multi Lake Survey (EMLS) in summer 2015 was an initiative among scientists from 27 countries to collect and analyse lake physical, chemical and biological variables in a fully standardized manner. This database includes in-situ lake variables along with nutrient, pigment and cyanotoxin data of 369 lakes in Europe, which were centrally analysed in dedicated laboratories. Publishing the EMLS methods and dataset might inspire similar initiatives to study across large geographic areas that will contribute to better understanding lake responses in a changing environment. Machine-accessible metadata file describing the reported data (ISA-Tab format)
Evanthia Mantzouki; James Campbell; Emiel van Loon; Petra Visser; Iosif Konstantinou; Maria Antoniou; Gregory Giuliani; Danielle Machado-Vieira; Alinne Gurjão de Oliveira; Dubravka Špoljarić Maronić; Filip Stević; Tanja Žuna Pfeiffer; Itana Bokan Vucelić; Petar Žutinić; Marija Gligora Udovič; Anđelka Plenković-Moraj; Nikoletta Tsiarta; Luděk Bláha; Rodan Geriš; Markéta Fránková; Kirsten Seestern Christoffersen; Trine Perlt Warming; Tõnu Feldmann; Alo Laas; Kristel Panksep; Lea Tuvikene; Kersti Kangro; Kerstin Häggqvist; Pauliina Salmi; Lauri Arvola; Jutta Fastner; Dietmar Straile; Karl-Otto Rothhaupt; Jeremy Fonvielle; Hans-Peter Grossart; Christos Avagianos; Triantafyllos Kaloudis; Theodoros Triantis; Sevasti-Kiriaki Zervou; Anastasia Hiskia; Spyros Gkelis; Manthos Panou; Valerie McCarthy; Victor C. Perello; Ulrike Obertegger; Adriano Boscaini; Giovanna Flaim; Nico Salmaso; Leonardo Cerasino; Judita Koreivienė; Jūratė Karosienė; Jūratė Kasperovičienė; Ksenija Savadova; Irma Vitonytė; Sigrid Haande; Birger Skjelbred; Magdalena Grabowska; Maciej Karpowicz; Damian Chmura; Lidia Nawrocka; Justyna Kobos; Hanna Mazur-Marzec; Pablo Alcaraz-Párraga; Elzbieta Wilk-Wozniak; Wojciech Krztoń; Edward Walusiak; Ilona Gagala; Joanna Mankiewicz-Boczek; Magdalena Toporowska; Barbara Pawlik-Skowronska; Michał Niedźwiecki; Wojciech Pęczuła; Agnieszka Napiórkowska-Krzebietke; Julita Dunalska; Justyna Sieńska; Daniel Szymański; Marek Kruk; Agnieszka Budzyńska; Ryszard Goldyn; Anna Kozak; Joanna Rosińska; Elżbieta Szeląg-Wasielewska; Piotr Domek; Natalia Jakubowska-Krepska; Kinga Kwasizur; Beata Messyasz; Aleksandra Pełechata; Mariusz Pełechaty; Mikolaj Kokocinski; Beata Madrecka; Iwona Kostrzewska-Szlakowska; Magdalena Frąk; Agnieszka Bańkowska-Sobczak; Michał Wasilewicz; Agnieszka Ochocka; Agnieszka Pasztaleniec; Iwona Jasser; Ana M. Antão-Geraldes; Manel Leira; Armand Hernández; Vitor Vasconcelos; João Morais; Micaela Vale; Pedro M. Raposeiro; Vítor Gonçalves; Boris Aleksovski; Svetislav Krstić; Hana Nemova; Iveta Drastichova; Lucia Chomova; Spela Remec-Rekar; Tina Elersek; Jordi Delgado-Martín; David García; Jose Luís Cereijo; Joan Gomà; Mari Carmen Trapote; Teresa Vegas-Vilarrúbia; Biel Obrador; Ana García-Murcia; Monserrat Real; Elvira Romans; Jordi Noguero-Ribes; David Parreño Duque; Elísabeth Fernández-Morán; Bárbara Úbeda; José Ángel Gálvez; Rafael Marcé; Núria Catalán; Carmen Pérez-Martínez; Eloísa Ramos-Rodríguez; Carmen Cillero-Castro; Enrique Moreno-Ostos; José María Blanco; Valeriano Rodríguez; Jorge Juan Montes-Pérez; Roberto L. Palomino; Estela Rodríguez-Pérez; Rafael Carballeira; Antonio Camacho; Antonio Picazo; Carlos Rochera; Anna C. Santamans; Carmen Ferriol; Susana Romo; Juan Soria; Lars-Anders Hansson; Pablo Urrutia-Cordero; Arda Özen; Andrea G. Bravo; Moritz Buck; William Colom-Montero; Kristiina Mustonen; Don Pierson; Yang Yang; Jolanda M. H. Verspagen; Lisette De Senerpont Domis; Laura Seelen; Sven Teurlincx; Yvon Verstijnen; Miquel Lürling; Valentini Maliaka; Elisabeth J. Faassen; Delphine Latour; Cayelan C. Carey; Hans W. Paerl; Andrea Torokne; Tünay Karan; Nilsun Demir; Meryem Beklioğlu; Nur Filiz; Eti E. Levi; Uğur Iskin; Gizem Bezirci; Ülkü Nihan Tavşanoğlu; Kemal Çelik; Koray Ozhan; Nusret Karakaya; Mehmet Ali Turan Koçer; Mete Yilmaz; Faruk Maraşlıoğlu; Özden Fakioglu; Elif Neyran Soylu; Meral Apaydın Yağcı; Şakir Çınar; Kadir Çapkın; Abdulkadir Yağcı; Mehmet Cesur; Fuat Bilgin; Cafer Bulut; Rahmi Uysal; Latife Köker; Reyhan Akçaalan; Meriç Albay; Mehmet Tahir Alp; Korhan Özkan; Tuğba Ongun Sevindik; Hatice Tunca; Burçin Önem; Jessica Richardson; Christine Edwards; Victoria Bergkemper; Sarah O'Leary; Eilish Beirne; Hannah Cromie; Bastiaan W. Ibelings. A European Multi Lake Survey dataset of environmental variables, phytoplankton pigments and cyanotoxins. Scientific Data 2018, 5, 1 -13.
AMA StyleEvanthia Mantzouki, James Campbell, Emiel van Loon, Petra Visser, Iosif Konstantinou, Maria Antoniou, Gregory Giuliani, Danielle Machado-Vieira, Alinne Gurjão de Oliveira, Dubravka Špoljarić Maronić, Filip Stević, Tanja Žuna Pfeiffer, Itana Bokan Vucelić, Petar Žutinić, Marija Gligora Udovič, Anđelka Plenković-Moraj, Nikoletta Tsiarta, Luděk Bláha, Rodan Geriš, Markéta Fránková, Kirsten Seestern Christoffersen, Trine Perlt Warming, Tõnu Feldmann, Alo Laas, Kristel Panksep, Lea Tuvikene, Kersti Kangro, Kerstin Häggqvist, Pauliina Salmi, Lauri Arvola, Jutta Fastner, Dietmar Straile, Karl-Otto Rothhaupt, Jeremy Fonvielle, Hans-Peter Grossart, Christos Avagianos, Triantafyllos Kaloudis, Theodoros Triantis, Sevasti-Kiriaki Zervou, Anastasia Hiskia, Spyros Gkelis, Manthos Panou, Valerie McCarthy, Victor C. Perello, Ulrike Obertegger, Adriano Boscaini, Giovanna Flaim, Nico Salmaso, Leonardo Cerasino, Judita Koreivienė, Jūratė Karosienė, Jūratė Kasperovičienė, Ksenija Savadova, Irma Vitonytė, Sigrid Haande, Birger Skjelbred, Magdalena Grabowska, Maciej Karpowicz, Damian Chmura, Lidia Nawrocka, Justyna Kobos, Hanna Mazur-Marzec, Pablo Alcaraz-Párraga, Elzbieta Wilk-Wozniak, Wojciech Krztoń, Edward Walusiak, Ilona Gagala, Joanna Mankiewicz-Boczek, Magdalena Toporowska, Barbara Pawlik-Skowronska, Michał Niedźwiecki, Wojciech Pęczuła, Agnieszka Napiórkowska-Krzebietke, Julita Dunalska, Justyna Sieńska, Daniel Szymański, Marek Kruk, Agnieszka Budzyńska, Ryszard Goldyn, Anna Kozak, Joanna Rosińska, Elżbieta Szeląg-Wasielewska, Piotr Domek, Natalia Jakubowska-Krepska, Kinga Kwasizur, Beata Messyasz, Aleksandra Pełechata, Mariusz Pełechaty, Mikolaj Kokocinski, Beata Madrecka, Iwona Kostrzewska-Szlakowska, Magdalena Frąk, Agnieszka Bańkowska-Sobczak, Michał Wasilewicz, Agnieszka Ochocka, Agnieszka Pasztaleniec, Iwona Jasser, Ana M. Antão-Geraldes, Manel Leira, Armand Hernández, Vitor Vasconcelos, João Morais, Micaela Vale, Pedro M. Raposeiro, Vítor Gonçalves, Boris Aleksovski, Svetislav Krstić, Hana Nemova, Iveta Drastichova, Lucia Chomova, Spela Remec-Rekar, Tina Elersek, Jordi Delgado-Martín, David García, Jose Luís Cereijo, Joan Gomà, Mari Carmen Trapote, Teresa Vegas-Vilarrúbia, Biel Obrador, Ana García-Murcia, Monserrat Real, Elvira Romans, Jordi Noguero-Ribes, David Parreño Duque, Elísabeth Fernández-Morán, Bárbara Úbeda, José Ángel Gálvez, Rafael Marcé, Núria Catalán, Carmen Pérez-Martínez, Eloísa Ramos-Rodríguez, Carmen Cillero-Castro, Enrique Moreno-Ostos, José María Blanco, Valeriano Rodríguez, Jorge Juan Montes-Pérez, Roberto L. Palomino, Estela Rodríguez-Pérez, Rafael Carballeira, Antonio Camacho, Antonio Picazo, Carlos Rochera, Anna C. Santamans, Carmen Ferriol, Susana Romo, Juan Soria, Lars-Anders Hansson, Pablo Urrutia-Cordero, Arda Özen, Andrea G. Bravo, Moritz Buck, William Colom-Montero, Kristiina Mustonen, Don Pierson, Yang Yang, Jolanda M. H. Verspagen, Lisette De Senerpont Domis, Laura Seelen, Sven Teurlincx, Yvon Verstijnen, Miquel Lürling, Valentini Maliaka, Elisabeth J. Faassen, Delphine Latour, Cayelan C. Carey, Hans W. Paerl, Andrea Torokne, Tünay Karan, Nilsun Demir, Meryem Beklioğlu, Nur Filiz, Eti E. Levi, Uğur Iskin, Gizem Bezirci, Ülkü Nihan Tavşanoğlu, Kemal Çelik, Koray Ozhan, Nusret Karakaya, Mehmet Ali Turan Koçer, Mete Yilmaz, Faruk Maraşlıoğlu, Özden Fakioglu, Elif Neyran Soylu, Meral Apaydın Yağcı, Şakir Çınar, Kadir Çapkın, Abdulkadir Yağcı, Mehmet Cesur, Fuat Bilgin, Cafer Bulut, Rahmi Uysal, Latife Köker, Reyhan Akçaalan, Meriç Albay, Mehmet Tahir Alp, Korhan Özkan, Tuğba Ongun Sevindik, Hatice Tunca, Burçin Önem, Jessica Richardson, Christine Edwards, Victoria Bergkemper, Sarah O'Leary, Eilish Beirne, Hannah Cromie, Bastiaan W. Ibelings. A European Multi Lake Survey dataset of environmental variables, phytoplankton pigments and cyanotoxins. Scientific Data. 2018; 5 (1):1-13.
Chicago/Turabian StyleEvanthia Mantzouki; James Campbell; Emiel van Loon; Petra Visser; Iosif Konstantinou; Maria Antoniou; Gregory Giuliani; Danielle Machado-Vieira; Alinne Gurjão de Oliveira; Dubravka Špoljarić Maronić; Filip Stević; Tanja Žuna Pfeiffer; Itana Bokan Vucelić; Petar Žutinić; Marija Gligora Udovič; Anđelka Plenković-Moraj; Nikoletta Tsiarta; Luděk Bláha; Rodan Geriš; Markéta Fránková; Kirsten Seestern Christoffersen; Trine Perlt Warming; Tõnu Feldmann; Alo Laas; Kristel Panksep; Lea Tuvikene; Kersti Kangro; Kerstin Häggqvist; Pauliina Salmi; Lauri Arvola; Jutta Fastner; Dietmar Straile; Karl-Otto Rothhaupt; Jeremy Fonvielle; Hans-Peter Grossart; Christos Avagianos; Triantafyllos Kaloudis; Theodoros Triantis; Sevasti-Kiriaki Zervou; Anastasia Hiskia; Spyros Gkelis; Manthos Panou; Valerie McCarthy; Victor C. Perello; Ulrike Obertegger; Adriano Boscaini; Giovanna Flaim; Nico Salmaso; Leonardo Cerasino; Judita Koreivienė; Jūratė Karosienė; Jūratė Kasperovičienė; Ksenija Savadova; Irma Vitonytė; Sigrid Haande; Birger Skjelbred; Magdalena Grabowska; Maciej Karpowicz; Damian Chmura; Lidia Nawrocka; Justyna Kobos; Hanna Mazur-Marzec; Pablo Alcaraz-Párraga; Elzbieta Wilk-Wozniak; Wojciech Krztoń; Edward Walusiak; Ilona Gagala; Joanna Mankiewicz-Boczek; Magdalena Toporowska; Barbara Pawlik-Skowronska; Michał Niedźwiecki; Wojciech Pęczuła; Agnieszka Napiórkowska-Krzebietke; Julita Dunalska; Justyna Sieńska; Daniel Szymański; Marek Kruk; Agnieszka Budzyńska; Ryszard Goldyn; Anna Kozak; Joanna Rosińska; Elżbieta Szeląg-Wasielewska; Piotr Domek; Natalia Jakubowska-Krepska; Kinga Kwasizur; Beata Messyasz; Aleksandra Pełechata; Mariusz Pełechaty; Mikolaj Kokocinski; Beata Madrecka; Iwona Kostrzewska-Szlakowska; Magdalena Frąk; Agnieszka Bańkowska-Sobczak; Michał Wasilewicz; Agnieszka Ochocka; Agnieszka Pasztaleniec; Iwona Jasser; Ana M. Antão-Geraldes; Manel Leira; Armand Hernández; Vitor Vasconcelos; João Morais; Micaela Vale; Pedro M. Raposeiro; Vítor Gonçalves; Boris Aleksovski; Svetislav Krstić; Hana Nemova; Iveta Drastichova; Lucia Chomova; Spela Remec-Rekar; Tina Elersek; Jordi Delgado-Martín; David García; Jose Luís Cereijo; Joan Gomà; Mari Carmen Trapote; Teresa Vegas-Vilarrúbia; Biel Obrador; Ana García-Murcia; Monserrat Real; Elvira Romans; Jordi Noguero-Ribes; David Parreño Duque; Elísabeth Fernández-Morán; Bárbara Úbeda; José Ángel Gálvez; Rafael Marcé; Núria Catalán; Carmen Pérez-Martínez; Eloísa Ramos-Rodríguez; Carmen Cillero-Castro; Enrique Moreno-Ostos; José María Blanco; Valeriano Rodríguez; Jorge Juan Montes-Pérez; Roberto L. Palomino; Estela Rodríguez-Pérez; Rafael Carballeira; Antonio Camacho; Antonio Picazo; Carlos Rochera; Anna C. Santamans; Carmen Ferriol; Susana Romo; Juan Soria; Lars-Anders Hansson; Pablo Urrutia-Cordero; Arda Özen; Andrea G. Bravo; Moritz Buck; William Colom-Montero; Kristiina Mustonen; Don Pierson; Yang Yang; Jolanda M. H. Verspagen; Lisette De Senerpont Domis; Laura Seelen; Sven Teurlincx; Yvon Verstijnen; Miquel Lürling; Valentini Maliaka; Elisabeth J. Faassen; Delphine Latour; Cayelan C. Carey; Hans W. Paerl; Andrea Torokne; Tünay Karan; Nilsun Demir; Meryem Beklioğlu; Nur Filiz; Eti E. Levi; Uğur Iskin; Gizem Bezirci; Ülkü Nihan Tavşanoğlu; Kemal Çelik; Koray Ozhan; Nusret Karakaya; Mehmet Ali Turan Koçer; Mete Yilmaz; Faruk Maraşlıoğlu; Özden Fakioglu; Elif Neyran Soylu; Meral Apaydın Yağcı; Şakir Çınar; Kadir Çapkın; Abdulkadir Yağcı; Mehmet Cesur; Fuat Bilgin; Cafer Bulut; Rahmi Uysal; Latife Köker; Reyhan Akçaalan; Meriç Albay; Mehmet Tahir Alp; Korhan Özkan; Tuğba Ongun Sevindik; Hatice Tunca; Burçin Önem; Jessica Richardson; Christine Edwards; Victoria Bergkemper; Sarah O'Leary; Eilish Beirne; Hannah Cromie; Bastiaan W. Ibelings. 2018. "A European Multi Lake Survey dataset of environmental variables, phytoplankton pigments and cyanotoxins." Scientific Data 5, no. 1: 1-13.
This paper presents the first report of cyanobacteria and cyanotoxins from the South Caucasus region, in particular from Lake Yerevan (Armenia). Microcystis, Dolichospermum, and Planktothrix were the key genera identified during the growing season. A trend of a remarkable increase in cyanobacterial densities was observed from 2012 to 2013 exhibiting bloom formation in June (by Nostoc linckia) with the highest values in June and August 2013, reaching up to 695.9*103 cells mL−1. Higher seasonal dependence of cyanobacterial density on temperature, and temperature as a driver for cyanobacterial cells growth and development were suggested. Biogenic nutrients were identified as co-drivers to determine species richness and dominance, as well as the distribution of phytoplankton in different parts of the reservoir. The highest cyanotoxin concentrations in the filtered biomass were reported during July 2012 for both stations of the reservoir (left and right bank). Microcystin-RR (MC-RR) was the most frequently observed cyanotoxin. Lower MC-LR concentrations were identified in all samples from both stations, with the highest values observed at the right bank in July 2012. [D-Asp3]MC-RR, MC-YR, MC-HtyR, [D-Asp3]MC-LR, MC-HilR, MC-WR, MC-LY and MC-LW were also identified in trace levels. Anatoxin-a (ANA) was reported in the samples from both stations during August. Cylindrospermopsin (CYN) was present in trace concentrations in samples from both stations during July and in the sample from the left bank during September.
Arevik Minasyan; Christophoros Christophoridis; Alan E. Wilson; Sevasti-Kiriaki Zervou; Triantafyllos Kaloudis; Anastasia Hiskia. Diversity of cyanobacteria and the presence of cyanotoxins in the epilimnion of Lake Yerevan (Armenia). Toxicon 2018, 150, 28 -38.
AMA StyleArevik Minasyan, Christophoros Christophoridis, Alan E. Wilson, Sevasti-Kiriaki Zervou, Triantafyllos Kaloudis, Anastasia Hiskia. Diversity of cyanobacteria and the presence of cyanotoxins in the epilimnion of Lake Yerevan (Armenia). Toxicon. 2018; 150 ():28-38.
Chicago/Turabian StyleArevik Minasyan; Christophoros Christophoridis; Alan E. Wilson; Sevasti-Kiriaki Zervou; Triantafyllos Kaloudis; Anastasia Hiskia. 2018. "Diversity of cyanobacteria and the presence of cyanotoxins in the epilimnion of Lake Yerevan (Armenia)." Toxicon 150, no. : 28-38.
Insight into how environmental change determines the production and distribution of cyanobacterial toxins is necessary for risk assessment. Management guidelines currently focus on hepatotoxins (microcystins). Increasing attention is given to other classes, such as neurotoxins (e.g., anatoxin-a) and cytotoxins (e.g., cylindrospermopsin) due to their potency. Most studies examine the relationship between individual toxin variants and environmental factors, such as nutrients, temperature and light. In summer 2015, we collected samples across Europe to investigate the effect of nutrient and temperature gradients on the variability of toxin production at a continental scale. Direct and indirect effects of temperature were the main drivers of the spatial distribution in the toxins produced by the cyanobacterial community, the toxin concentrations and toxin quota. Generalized linear models showed that a Toxin Diversity Index (TDI) increased with latitude, while it decreased with water stability. Increases in TDI were explained through a significant increase in toxin variants such as MC-YR, anatoxin and cylindrospermopsin, accompanied by a decreasing presence of MC-LR. While global warming continues, the direct and indirect effects of increased lake temperatures will drive changes in the distribution of cyanobacterial toxins in Europe, potentially promoting selection of a few highly toxic species or strains.
Evanthia Mantzouki; Miquel Lürling; Jutta Fastner; Lisette De Senerpont Domis; Elżbieta Wilk-Woźniak; Judita Koreivienė; Laura Seelen; Sven Teurlincx; Yvon Verstijnen; Wojciech Krztoń; Edward Walusiak; Jūratė Karosienė; Jūratė Kasperovičienė; Ksenija Savadova; Irma Vitonytė; Carmen Cillero-Castro; Agnieszka Budzyńska; Ryszard Goldyn; Anna Kozak; Joanna Rosińska; Elżbieta Szeląg-Wasielewska; Piotr Domek; Natalia Jakubowska-Krepska; Kinga Kwasizur; Beata Messyasz; Aleksandra Pełechata; Mariusz Pełechaty; Mikolaj Kokocinski; Ana García-Murcia; Monserrat Real; Elvira Romans; Jordi Noguero-Ribes; David Parreño Duque; Elísabeth Fernández-Morán; Nusret Karakaya; Kerstin Häggqvist; Nilsun Demir; Meryem Beklioğlu; Nur Filiz; Eti E. Levi; Uğur Iskin; Gizem Bezirci; Ülkü Nihan Tavşanoğlu; Koray Özhan; Spyros Gkelis; Manthos Panou; Özden Fakioglu; Christos Avagianos; Triantafyllos Kaloudis; Kemal Çelik; Mete Yilmaz; Rafael Marcé; Nuria Catalán; Andrea G. Bravo; Moritz Buck; William Colom-Montero; Kristiina Mustonen; Don Pierson; Yang Yang; Pedro M. Raposeiro; Vítor Gonçalves; Maria G. Antoniou; Nikoletta Tsiarta; Valerie McCarthy; Victor C. Perello; Tõnu Feldmann; Alo Laas; Kristel Panksep; Lea Tuvikene; Ilona Gagala; Joana Mankiewicz-Boczek; Meral Apaydın Yağcı; Şakir Çınar; Kadir Çapkın; Abdulkadir Yağcı; Mehmet Cesur; Fuat Bilgin; Cafer Bulut; Rahmi Uysal; Ulrike Obertegger; Adriano Boscaini; Giovanna Flaim; Nico Salmaso; Leonardo Cerasino; Jessica Richardson; Petra M. Visser; Jolanda M. H. Verspagen; Tünay Karan; Elif Neyran Soylu; Faruk Maraşlıoğlu; Agnieszka Napiórkowska-Krzebietke; Agnieszka Ochocka; Agnieszka Pasztaleniec; Ana M. Antão-Geraldes; Vitor Vasconcelos; João Morais; Micaela Vale; Latife Köker; Reyhan Akçaalan; Meriç Albay; Dubravka Špoljarić Maronić; Filip Stević; Tanja Žuna Pfeiffer; Jeremy Fonvielle; Dietmar Straile; Karl-Otto Rothhaupt; Lars-Anders Hansson; Pablo Urrutia-Cordero; Luděk Bláha; Rodan Geriš; Markéta Fránková; Mehmet Ali Turan Koçer; Mehmet Tahir Alp; Spela Remec-Rekar; Tina Elersek; Theodoros Triantis; Sevasti-Kiriaki Zervou; Anastasia Hiskia; Sigrid Haande; Birger Skjelbred; Beata Madrecka; Hana Nemova; Iveta Drastichova; Lucia Chomova; Christine Edwards; Tuğba Ongun Sevindik; Hatice Tunca; Burçin Önem; Boris Aleksovski; Svetislav Krstić; Itana Bokan Vucelić; Lidia Nawrocka; Pauliina Salmi; Danielle Machado-Vieira; Alinne Gurjão De Oliveira; Jordi Delgado-Martín; David García; Jose Luís Cereijo; Joan Gomà; Mari Carmen Trapote; Teresa Vegas-Vilarrúbia; Biel Obrador; Magdalena Grabowska; Maciej Karpowicz; Damian Chmura; Bárbara Úbeda; José Ángel Gálvez; Arda Özen; Kirsten Seestern Christoffersen; Trine Perlt Warming; Justyna Kobos; Hanna Mazur-Marzec; Carmen Pérez-Martínez; Eloísa Ramos-Rodríguez; Lauri Arvola; Pablo Alcaraz-Párraga; Magdalena Toporowska; Barbara Pawlik-Skowronska; Michał Niedźwiecki; Wojciech Pęczuła; Manel Leira; Armand Hernández; Enrique Moreno-Ostos; José María Blanco; Valeriano Rodríguez; Jorge Juan Montes-Pérez; Roberto L. Palomino; Estela Rodríguez-Pérez; Rafael Carballeira; Antonio Camacho; Antonio Picazo; Carlos Rochera; Anna C. Santamans; Carmen Ferriol; Susana Romo; Juan Miguel Soria; Julita Dunalska; Justyna Sieńska; Daniel Szymański; Marek Kruk; Iwona Kostrzewska-Szlakowska; Iwona Jasser; Petar Žutinić; Marija Gligora Udovič; Anđelka Plenković-Moraj; Magdalena Frąk; Agnieszka Bańkowska-Sobczak; Michał Wasilewicz; Korhan Özkan; Valentini Maliaka; Kersti Kangro; Hans-Peter Grossart; Hans Paerl; Cayelan C. Carey; Bas W. Ibelings. Temperature Effects Explain Continental Scale Distribution of Cyanobacterial Toxins. Toxins 2018, 10, 156 .
AMA StyleEvanthia Mantzouki, Miquel Lürling, Jutta Fastner, Lisette De Senerpont Domis, Elżbieta Wilk-Woźniak, Judita Koreivienė, Laura Seelen, Sven Teurlincx, Yvon Verstijnen, Wojciech Krztoń, Edward Walusiak, Jūratė Karosienė, Jūratė Kasperovičienė, Ksenija Savadova, Irma Vitonytė, Carmen Cillero-Castro, Agnieszka Budzyńska, Ryszard Goldyn, Anna Kozak, Joanna Rosińska, Elżbieta Szeląg-Wasielewska, Piotr Domek, Natalia Jakubowska-Krepska, Kinga Kwasizur, Beata Messyasz, Aleksandra Pełechata, Mariusz Pełechaty, Mikolaj Kokocinski, Ana García-Murcia, Monserrat Real, Elvira Romans, Jordi Noguero-Ribes, David Parreño Duque, Elísabeth Fernández-Morán, Nusret Karakaya, Kerstin Häggqvist, Nilsun Demir, Meryem Beklioğlu, Nur Filiz, Eti E. Levi, Uğur Iskin, Gizem Bezirci, Ülkü Nihan Tavşanoğlu, Koray Özhan, Spyros Gkelis, Manthos Panou, Özden Fakioglu, Christos Avagianos, Triantafyllos Kaloudis, Kemal Çelik, Mete Yilmaz, Rafael Marcé, Nuria Catalán, Andrea G. Bravo, Moritz Buck, William Colom-Montero, Kristiina Mustonen, Don Pierson, Yang Yang, Pedro M. Raposeiro, Vítor Gonçalves, Maria G. Antoniou, Nikoletta Tsiarta, Valerie McCarthy, Victor C. Perello, Tõnu Feldmann, Alo Laas, Kristel Panksep, Lea Tuvikene, Ilona Gagala, Joana Mankiewicz-Boczek, Meral Apaydın Yağcı, Şakir Çınar, Kadir Çapkın, Abdulkadir Yağcı, Mehmet Cesur, Fuat Bilgin, Cafer Bulut, Rahmi Uysal, Ulrike Obertegger, Adriano Boscaini, Giovanna Flaim, Nico Salmaso, Leonardo Cerasino, Jessica Richardson, Petra M. Visser, Jolanda M. H. Verspagen, Tünay Karan, Elif Neyran Soylu, Faruk Maraşlıoğlu, Agnieszka Napiórkowska-Krzebietke, Agnieszka Ochocka, Agnieszka Pasztaleniec, Ana M. Antão-Geraldes, Vitor Vasconcelos, João Morais, Micaela Vale, Latife Köker, Reyhan Akçaalan, Meriç Albay, Dubravka Špoljarić Maronić, Filip Stević, Tanja Žuna Pfeiffer, Jeremy Fonvielle, Dietmar Straile, Karl-Otto Rothhaupt, Lars-Anders Hansson, Pablo Urrutia-Cordero, Luděk Bláha, Rodan Geriš, Markéta Fránková, Mehmet Ali Turan Koçer, Mehmet Tahir Alp, Spela Remec-Rekar, Tina Elersek, Theodoros Triantis, Sevasti-Kiriaki Zervou, Anastasia Hiskia, Sigrid Haande, Birger Skjelbred, Beata Madrecka, Hana Nemova, Iveta Drastichova, Lucia Chomova, Christine Edwards, Tuğba Ongun Sevindik, Hatice Tunca, Burçin Önem, Boris Aleksovski, Svetislav Krstić, Itana Bokan Vucelić, Lidia Nawrocka, Pauliina Salmi, Danielle Machado-Vieira, Alinne Gurjão De Oliveira, Jordi Delgado-Martín, David García, Jose Luís Cereijo, Joan Gomà, Mari Carmen Trapote, Teresa Vegas-Vilarrúbia, Biel Obrador, Magdalena Grabowska, Maciej Karpowicz, Damian Chmura, Bárbara Úbeda, José Ángel Gálvez, Arda Özen, Kirsten Seestern Christoffersen, Trine Perlt Warming, Justyna Kobos, Hanna Mazur-Marzec, Carmen Pérez-Martínez, Eloísa Ramos-Rodríguez, Lauri Arvola, Pablo Alcaraz-Párraga, Magdalena Toporowska, Barbara Pawlik-Skowronska, Michał Niedźwiecki, Wojciech Pęczuła, Manel Leira, Armand Hernández, Enrique Moreno-Ostos, José María Blanco, Valeriano Rodríguez, Jorge Juan Montes-Pérez, Roberto L. Palomino, Estela Rodríguez-Pérez, Rafael Carballeira, Antonio Camacho, Antonio Picazo, Carlos Rochera, Anna C. Santamans, Carmen Ferriol, Susana Romo, Juan Miguel Soria, Julita Dunalska, Justyna Sieńska, Daniel Szymański, Marek Kruk, Iwona Kostrzewska-Szlakowska, Iwona Jasser, Petar Žutinić, Marija Gligora Udovič, Anđelka Plenković-Moraj, Magdalena Frąk, Agnieszka Bańkowska-Sobczak, Michał Wasilewicz, Korhan Özkan, Valentini Maliaka, Kersti Kangro, Hans-Peter Grossart, Hans Paerl, Cayelan C. Carey, Bas W. Ibelings. Temperature Effects Explain Continental Scale Distribution of Cyanobacterial Toxins. Toxins. 2018; 10 (4):156.
Chicago/Turabian StyleEvanthia Mantzouki; Miquel Lürling; Jutta Fastner; Lisette De Senerpont Domis; Elżbieta Wilk-Woźniak; Judita Koreivienė; Laura Seelen; Sven Teurlincx; Yvon Verstijnen; Wojciech Krztoń; Edward Walusiak; Jūratė Karosienė; Jūratė Kasperovičienė; Ksenija Savadova; Irma Vitonytė; Carmen Cillero-Castro; Agnieszka Budzyńska; Ryszard Goldyn; Anna Kozak; Joanna Rosińska; Elżbieta Szeląg-Wasielewska; Piotr Domek; Natalia Jakubowska-Krepska; Kinga Kwasizur; Beata Messyasz; Aleksandra Pełechata; Mariusz Pełechaty; Mikolaj Kokocinski; Ana García-Murcia; Monserrat Real; Elvira Romans; Jordi Noguero-Ribes; David Parreño Duque; Elísabeth Fernández-Morán; Nusret Karakaya; Kerstin Häggqvist; Nilsun Demir; Meryem Beklioğlu; Nur Filiz; Eti E. Levi; Uğur Iskin; Gizem Bezirci; Ülkü Nihan Tavşanoğlu; Koray Özhan; Spyros Gkelis; Manthos Panou; Özden Fakioglu; Christos Avagianos; Triantafyllos Kaloudis; Kemal Çelik; Mete Yilmaz; Rafael Marcé; Nuria Catalán; Andrea G. Bravo; Moritz Buck; William Colom-Montero; Kristiina Mustonen; Don Pierson; Yang Yang; Pedro M. Raposeiro; Vítor Gonçalves; Maria G. Antoniou; Nikoletta Tsiarta; Valerie McCarthy; Victor C. Perello; Tõnu Feldmann; Alo Laas; Kristel Panksep; Lea Tuvikene; Ilona Gagala; Joana Mankiewicz-Boczek; Meral Apaydın Yağcı; Şakir Çınar; Kadir Çapkın; Abdulkadir Yağcı; Mehmet Cesur; Fuat Bilgin; Cafer Bulut; Rahmi Uysal; Ulrike Obertegger; Adriano Boscaini; Giovanna Flaim; Nico Salmaso; Leonardo Cerasino; Jessica Richardson; Petra M. Visser; Jolanda M. H. Verspagen; Tünay Karan; Elif Neyran Soylu; Faruk Maraşlıoğlu; Agnieszka Napiórkowska-Krzebietke; Agnieszka Ochocka; Agnieszka Pasztaleniec; Ana M. Antão-Geraldes; Vitor Vasconcelos; João Morais; Micaela Vale; Latife Köker; Reyhan Akçaalan; Meriç Albay; Dubravka Špoljarić Maronić; Filip Stević; Tanja Žuna Pfeiffer; Jeremy Fonvielle; Dietmar Straile; Karl-Otto Rothhaupt; Lars-Anders Hansson; Pablo Urrutia-Cordero; Luděk Bláha; Rodan Geriš; Markéta Fránková; Mehmet Ali Turan Koçer; Mehmet Tahir Alp; Spela Remec-Rekar; Tina Elersek; Theodoros Triantis; Sevasti-Kiriaki Zervou; Anastasia Hiskia; Sigrid Haande; Birger Skjelbred; Beata Madrecka; Hana Nemova; Iveta Drastichova; Lucia Chomova; Christine Edwards; Tuğba Ongun Sevindik; Hatice Tunca; Burçin Önem; Boris Aleksovski; Svetislav Krstić; Itana Bokan Vucelić; Lidia Nawrocka; Pauliina Salmi; Danielle Machado-Vieira; Alinne Gurjão De Oliveira; Jordi Delgado-Martín; David García; Jose Luís Cereijo; Joan Gomà; Mari Carmen Trapote; Teresa Vegas-Vilarrúbia; Biel Obrador; Magdalena Grabowska; Maciej Karpowicz; Damian Chmura; Bárbara Úbeda; José Ángel Gálvez; Arda Özen; Kirsten Seestern Christoffersen; Trine Perlt Warming; Justyna Kobos; Hanna Mazur-Marzec; Carmen Pérez-Martínez; Eloísa Ramos-Rodríguez; Lauri Arvola; Pablo Alcaraz-Párraga; Magdalena Toporowska; Barbara Pawlik-Skowronska; Michał Niedźwiecki; Wojciech Pęczuła; Manel Leira; Armand Hernández; Enrique Moreno-Ostos; José María Blanco; Valeriano Rodríguez; Jorge Juan Montes-Pérez; Roberto L. Palomino; Estela Rodríguez-Pérez; Rafael Carballeira; Antonio Camacho; Antonio Picazo; Carlos Rochera; Anna C. Santamans; Carmen Ferriol; Susana Romo; Juan Miguel Soria; Julita Dunalska; Justyna Sieńska; Daniel Szymański; Marek Kruk; Iwona Kostrzewska-Szlakowska; Iwona Jasser; Petar Žutinić; Marija Gligora Udovič; Anđelka Plenković-Moraj; Magdalena Frąk; Agnieszka Bańkowska-Sobczak; Michał Wasilewicz; Korhan Özkan; Valentini Maliaka; Kersti Kangro; Hans-Peter Grossart; Hans Paerl; Cayelan C. Carey; Bas W. Ibelings. 2018. "Temperature Effects Explain Continental Scale Distribution of Cyanobacterial Toxins." Toxins 10, no. 4: 156.
5',8-Cyclo-2'-deoxyadenosine (cdA) and 5',8-cyclo-2'-deoxyguanosine (cdG) in their two diastereomeric forms, 5'S and 5'R, are tandem lesions produced by the attack of hydroxyl radicals to the purine moieties of DNA. Their formation has been found to challenge the cells' repair machinery, initiating the nucleotide excision repair (NER) for restoring the genome integrity. The involvement of oxidatively induced DNA damage in carcinogenesis and the reduced capacity of some cancer cell lines to repair oxidized DNA base lesions, intrigued us to investigate the implication of these lesions in breast cancer, the most frequently occurring cancer in women. Using liquid chromatography tandem mass spectrometry (LC-MS/MS) we measured the levels of diastereomeric cdA's and cdG's in estrogen receptor-alpha positive (ER-α) MCF-7 and triple negative MDA-MB-231 breast cancer cell lines before and after exposure to two different conditions: ionizing radiations and hydrogen peroxide, followed by an interval period to allow DNA repair. An increase at the measured levels of all four lesions, i.e., 5'S-cdA, 5'R-cdA, 5'S-cdG and 5'R-cdG, was observed either after γ-irradiation (5 Gy dose) or hydrogen peroxide treatment (300 μM) compared to the untreated cells (control), independently from the length of the interval period for repair. For comparison reasons, we also measured the levels of 8-oxo-2'-deoxyadenosine (8-oxo-dA), a well known oxidatively induced DNA damage lesion and base excision repair (BER) substrate. The collected data indicate that MCF-7 and MDA-MB-231 breast cancer cells are highly susceptible to radiation-induced DNA damage, being mainly defective in the repair of these lesions.
Marios G. Krokidis; Michael A. Terzidis; Eleni Efthimiadou; Sevasti-Kiriaki Zervou; George Kordas; Kyriakos Papadopoulos; Anastasia Hiskia; Dimitris Kletsas; Chryssostomos Chatgilialoglu. Purine 5?,8-Cyclo-2?-deoxynucleoside Lesions: Formation by Radical Stress and Repair in Human Breast Epithelial Cancer Cells. Free Radical Research 2017, 51, 1 -28.
AMA StyleMarios G. Krokidis, Michael A. Terzidis, Eleni Efthimiadou, Sevasti-Kiriaki Zervou, George Kordas, Kyriakos Papadopoulos, Anastasia Hiskia, Dimitris Kletsas, Chryssostomos Chatgilialoglu. Purine 5?,8-Cyclo-2?-deoxynucleoside Lesions: Formation by Radical Stress and Repair in Human Breast Epithelial Cancer Cells. Free Radical Research. 2017; 51 (5):1-28.
Chicago/Turabian StyleMarios G. Krokidis; Michael A. Terzidis; Eleni Efthimiadou; Sevasti-Kiriaki Zervou; George Kordas; Kyriakos Papadopoulos; Anastasia Hiskia; Dimitris Kletsas; Chryssostomos Chatgilialoglu. 2017. "Purine 5?,8-Cyclo-2?-deoxynucleoside Lesions: Formation by Radical Stress and Repair in Human Breast Epithelial Cancer Cells." Free Radical Research 51, no. 5: 1-28.
Lake Karla (Central Greece) is a unique example - at European scale - of a shallow lake ecosystem that was dried in the 1960s and in 2009 started to be restored. The lake is listed in the network of the Greek protected areas as it is considered a vital aquatic ecosystem, in terms of biodiversity. It has, however, already been adversely affected by both agricultural and industrial land uses in the surrounding area, leading to eutrophication and shifting algal community towards bloom-forming toxic cyanobacterial species. After repeated heavy-blooms, cyanotoxin occurrence and mass fish kills, the local ecosystem management authority has implemented a water quality monitoring program (July 2013 - July 2015) to assess environmental pressures and the response of aquatic biota in the lake. Microscopic, immunological, and molecular techniques combined with physico-chemical parameters, complemented by liquid chromatography tandem mass spectrometry (LC-MS/MS), were used to monitor cyanobacteria blooms and the associated cyanotoxin production from three different sites in Lake Karla and from the adjacent Kalamaki Reservoir. Water quality was also assessed by the structure of benthic invertebrate community on the sediment. Cyanobacteria were the main phytoplankton component, representing more than 70% of the total phytoplankton abundance; dominant taxa belonged to Cylindrospermopsis raciborskii, Limnothrix redekei, Anabaenopsis elenkinii, and Microcystis spp. Euglenophytes (Euglena), diatoms (Nitzschia), and chlorophytes (Scenedesmus) were also important phytoplankton constituents. LC-MS/MS confirmed the co-occurrence of microcystins, cylindrospermopsin, saxitoxin, neo-saxitoxin and anatoxin-a. The occurrence of cyanotoxins in relation to the persistent and dominant cyanobacteria and the impact of cyanobacterial harmful algal blooms on the newly constructed lake along with the land uses and the emergent mitigation measures are discussed.
Spyros Gkelis; Manthos Panou; Ioannis Chronis; Sevasti-Kiriaki Zervou; Christophoros Christophoridis; Korina Manolidi; Chrysoula Ntislidou; Theodoros M. Triantis; Triantafyllos Kaloudis; Anastasia Hiskia; Ifigenia Kagalou; Maria Lazaridou. Monitoring a newly re-born patient: water quality and cyanotoxin occurrence in a reconstructed shallow Mediterranean lake. Advances in Oceanography and Limnology 2017, 8, 1 .
AMA StyleSpyros Gkelis, Manthos Panou, Ioannis Chronis, Sevasti-Kiriaki Zervou, Christophoros Christophoridis, Korina Manolidi, Chrysoula Ntislidou, Theodoros M. Triantis, Triantafyllos Kaloudis, Anastasia Hiskia, Ifigenia Kagalou, Maria Lazaridou. Monitoring a newly re-born patient: water quality and cyanotoxin occurrence in a reconstructed shallow Mediterranean lake. Advances in Oceanography and Limnology. 2017; 8 (1):1.
Chicago/Turabian StyleSpyros Gkelis; Manthos Panou; Ioannis Chronis; Sevasti-Kiriaki Zervou; Christophoros Christophoridis; Korina Manolidi; Chrysoula Ntislidou; Theodoros M. Triantis; Triantafyllos Kaloudis; Anastasia Hiskia; Ifigenia Kagalou; Maria Lazaridou. 2017. "Monitoring a newly re-born patient: water quality and cyanotoxin occurrence in a reconstructed shallow Mediterranean lake." Advances in Oceanography and Limnology 8, no. 1: 1.
Cyanobacterial and algal toxins comprise a large group of harmful metabolites, belonging to different chemical classes, with a variety of chemical structures, physicochemical properties and toxic activities. In this study, a fast, simple and sensitive analytical method was developed for the simultaneous determination of multi-class cyanobacterial and algal toxins in water. The target compounds were: Cylindrospermopsin, Anatoxin-a, Nodularin, 12 Microcystins ([D-Asp3]MC-RR, MC-RR, MC-YR, MC-HtyR, [D-Asp3]MC-LR, MC-LR, MC-HilR, MC-WR, MC-LA, MC-LY, MC-LW and MC-LF), Okadaic acid and Domoic acid. Analytes were determined using liquid chromatography-tandem mass spectrometry (LC-MS/MS). A dual Solid Phase Extraction (SPE) cartridge assembly was applied for the extraction of target compounds from water. Optimized SPE parameters included cartridge material, initial sample pH, sequence of the cartridges in the SPE assembly as well as composition and volume of the elution solvent. The method was validated, providing acceptable mean recoveries and reproducibility for most analytes. Limits of detection were at the ngL level. The method was successfully applied in real lake water samples from Greece, where a wide range of Microcystins were detected for the first time, at concentrations ranging from 0.034 to 63μgL.
Sevasti-Kiriaki Zervou; Christophoros Christophoridis; Triantafyllos Kaloudis; Theodoros Triantis; Anastasia Hiskia. New SPE-LC-MS/MS method for simultaneous determination of multi-class cyanobacterial and algal toxins. Journal of Hazardous Materials 2017, 323, 56 -66.
AMA StyleSevasti-Kiriaki Zervou, Christophoros Christophoridis, Triantafyllos Kaloudis, Theodoros Triantis, Anastasia Hiskia. New SPE-LC-MS/MS method for simultaneous determination of multi-class cyanobacterial and algal toxins. Journal of Hazardous Materials. 2017; 323 ():56-66.
Chicago/Turabian StyleSevasti-Kiriaki Zervou; Christophoros Christophoridis; Triantafyllos Kaloudis; Theodoros Triantis; Anastasia Hiskia. 2017. "New SPE-LC-MS/MS method for simultaneous determination of multi-class cyanobacterial and algal toxins." Journal of Hazardous Materials 323, no. : 56-66.
The cyanobacterium Aphanizomenon favaloroi was identified for the first time in Europe in the Mediterranean brackish Lake Vistonis during July–August 2014. It formed a dense bloom (from 16×106 to 81×106 trichomes L–1), causing a brown colouration of the lake water. When A. favaloroi formed 100% of the total phytoplankton biomass (44mg L–1), saxitoxins (saxitoxin and neo-saxitoxin) were detected in the lake seston (42 and 17μg g–1 phytoplankton dry weight respectively), which was screened for saxitoxins, cylindrospermopsin, anatoxin-a, microcystins and nodularin. A massive fish kill coincided with the A. favaloroi bloom. This new saxitoxin-producing species of cyanobacteria, with traits for successful dispersal, may pose a health risk to animals and humans and cause adverse effects on water quality and water services because of its expansion potential.
Maria Moustaka-Gouni; Anastasia Hiskia; Savvas Genitsaris; Matina Katsiapi; Korina Manolidi; Sevasti-Kiriaki Zervou; Christophoros Christophoridis; Theodoros M. Triantis; Triantafyllos Kaloudis; Sotiris Orfanidis; And. First report of Aphanizomenon favaloroi occurrence in Europe associated with saxitoxins and a massive fish kill in Lake Vistonis, Greece. Marine and Freshwater Research 2017, 68, 793 .
AMA StyleMaria Moustaka-Gouni, Anastasia Hiskia, Savvas Genitsaris, Matina Katsiapi, Korina Manolidi, Sevasti-Kiriaki Zervou, Christophoros Christophoridis, Theodoros M. Triantis, Triantafyllos Kaloudis, Sotiris Orfanidis, And. First report of Aphanizomenon favaloroi occurrence in Europe associated with saxitoxins and a massive fish kill in Lake Vistonis, Greece. Marine and Freshwater Research. 2017; 68 (4):793.
Chicago/Turabian StyleMaria Moustaka-Gouni; Anastasia Hiskia; Savvas Genitsaris; Matina Katsiapi; Korina Manolidi; Sevasti-Kiriaki Zervou; Christophoros Christophoridis; Theodoros M. Triantis; Triantafyllos Kaloudis; Sotiris Orfanidis; And. 2017. "First report of Aphanizomenon favaloroi occurrence in Europe associated with saxitoxins and a massive fish kill in Lake Vistonis, Greece." Marine and Freshwater Research 68, no. 4: 793.
A method for the determination of the hepatotoxic cyanotoxins microcystins (MCs, i.e. MC-LR, MC-RR, MC-YR, MC-LA) and nodularin (NOD) in water was developed using liquid chromatography with electrospray ionization triple quadrupole mass spectrometry (LC-ESI-MS/MS) after solid phase extraction (SPE). New patterns of fragmentation of MC-LA were observed under the experimental conditions used. The method was fully validated to meet accreditation criteria. Mean recoveries at three concentration levels (0.006, 0.1 and 1 μg L(-1)) ranged between 70 and 114% with %RSD values generally below 20%. Detection limits were 2 ng L(-1) for all hepatotoxins. The method was applied to study the occurrence of MCs and NOD in Lake Marathonas, a water reservoir of Athens, over a period from July 2007 to December 2010. The protein phosphatase inhibition assay (PPIA) was additionally used for fast screening of samples. MC-YR, MC-LR and MC-RR were detected and found to vary seasonally with consistent peaks during early autumn, having maximum concentrations of 717, 451 and 174 ng L(-1), respectively. The results of this study constitute the first report on the presence, concentration levels and seasonal variations of MCs in Lake Marathonas. None of the target cyanotoxins were detected in treated drinking water samples during the period of the study.
Triantafyllos Kaloudis; Sevasti-Kiriaki Zervou; Katerina Tsimeli; Theodoros M. Triantis; Theodora Fotiou; Anastasia Hiskia. Determination of microcystins and nodularin (cyanobacterial toxins) in water by LC–MS/MS. Monitoring of Lake Marathonas, a water reservoir of Athens, Greece. Journal of Hazardous Materials 2013, 263, 105 -115.
AMA StyleTriantafyllos Kaloudis, Sevasti-Kiriaki Zervou, Katerina Tsimeli, Theodoros M. Triantis, Theodora Fotiou, Anastasia Hiskia. Determination of microcystins and nodularin (cyanobacterial toxins) in water by LC–MS/MS. Monitoring of Lake Marathonas, a water reservoir of Athens, Greece. Journal of Hazardous Materials. 2013; 263 ():105-115.
Chicago/Turabian StyleTriantafyllos Kaloudis; Sevasti-Kiriaki Zervou; Katerina Tsimeli; Theodoros M. Triantis; Theodora Fotiou; Anastasia Hiskia. 2013. "Determination of microcystins and nodularin (cyanobacterial toxins) in water by LC–MS/MS. Monitoring of Lake Marathonas, a water reservoir of Athens, Greece." Journal of Hazardous Materials 263, no. : 105-115.