According to recent studies, Norway spruce cones and eastern hemlock cones possess exceptionally high antioxidant capacities; however, the antioxidant efficiency of extractives and their bioactive effects have yet to be investigated in detail. The present article utilizes state-of-the art analytical chemical methodology and chemometric evaluation to reveal cone extractives with the highest antioxidant potential, accounting for possible bioactive effects. Antioxidant properties were determined by the Ferric reducing antioxidant power (FRAP) assay and the Folin–Ciocalteu’s total phenol content (TPC) assay. Structure and relative polyphenol concentrations were determined using liquid chromatography/diode array detection/tandem mass spectrometry. The antibacterial testing was conducted using Staphylococcus aureus and Escherichia coli strains. Using correlation analysis and factor analysis, a scoring evaluation was implemented to determine and compare the antioxidant efficiency of extractives. In Norway spruce, piceatannol-O-hexoside and coumaric acid derivatives were found to be the most powerful antioxidants, while in eastern hemlock, kaempferol glycosides were the most powerful. The antibacterial test did not show any promising antimicrobial activity against E. coli, although a visible inhibitory effect on S. aureus strain was observed.

Natural matrices have historically been a cornerstone in drug discovery, offering a rich source of structurally diverse and biologically active compounds. However, research on natural products often faces significant challenges due to the complexity of natural matrices, such as urine, and the limitations of bioactivity assessment assays. To ensure reliable insights, it is crucial to optimize experimental conditions to reveal the bioactive potential of samples, thereby improving the validity of statistical analyses. Approaches in metabolomics further strengthen this process by identifying and focusing on the most promising compounds within natural matrices, enhancing the precision of bioactive metabolite prioritization. In this study, we assessed the bioactivity of 17 dromedary urine samples on human renal cells under serum-reduced conditions (1%FBS) in order to minimize possible FBS-derived interfering factors. Using viability assays and Annexin V/PI staining, we found that the tumor renal cell lines Caki-1 and RCC-Shaw were more sensitive to the cytotoxic effects of the small molecules present in dromedary urine compared to non-tumor HK-2 cells. Employing NMR metabolomics analysis combined with detected in vitro activity, our statistical model highlights the presence of bioactive compounds in dromedary urine, such as azelaic acid and phenylacetyl glycine, underscoring its potential as a sustainable source of bioactive molecules within the framework of green chemistry and circular economy initiatives.

Background: The presence of light-pigmented facial stripes, parallel on both sides of the cranial region, is a widespread characteristic in various goat breeds of European origin and beyond. In Italy, this phenotype is relatively common from the north to the south of the peninsula. The availability of genotypic data at single-nucleotide polymorphism (SNP) loci for breeds and populations characterized by such a pigmentation pattern enabled us to study the genomic regions potentially correlated with this phenotype, for simplicity referred to as “facciuto”. Methods: We adopted an F_ST-outlier approach to detect signals of differential selection in 18 pairwise comparisons, each involving 6 genetic goat types with the “facciuto” phenotype (Facciuta Lucana, Facciuta della Valnerina, Valfortorina, Teramana, Capestrina, and Roccaverano) contrasted with each of 3 “non-facciuto” goat breeds selected as reference populations (Red Mediterranean, light brown; Saanen, white; Malagueña, mahogany solid). Results: The analysis of the region ±200 kbps upstream and downstream of the two significant signals on chromosome 13 and 15 allowed us to identify, among the annotated genes, ASIP, AHCY, ITCH, DYNLRB1, MAP1LC3A, PIGU, LOC102177263, and DTX4, whose functions could be related to several mechanisms underlying the phenotype under investigation. Conclusions: This study confirmed the fundamental role of ASIP in pigmentation, although additional pathways may concurrently contribute to the determinism of the considered “facciuto” phenotype in Italian goats.