The aim of this study was to histologically and histomorphometrically investigate the effect of locally applied bovine amniotic fluid (BAF) on osseointegration levels in implants. Adult female Sprague–Dawley rats weighing 300–350 g were used as subjects. The rats were divided into two groups: the sham–operated control group (n=10) and the local BAF group (n=10). Implant cavities were created in the tibias of all subjects under sterile saline cooling with rotating instruments. Local BAF was applied to all implant sockets before the implants were placed. Rats were sacrificed after a four–week osseointegration period. Histological staining was performed using hematoxylin and eosin staining to analyze the osseointegration. Examinations of the bone implant connection (BIC) and peri–implant bone formation (PBF) were performed using a light microscope and an image analyzer. As a result of the analysis, the mean BIC value was 40.3 ± 4.9 for the sham–operated control group and 45.2 ± 7.7 for the local BAF group. The mean PBF was 39.9 ± 6.3 for the sham control group and 40.5 ± 5.7 for the local BAF group. A statistically significant difference was found between the sham control group and the local BAF group for the BIC and PBF values (P>0.05; P: 0.11; P: 0.83). The application of local BAF to the implant socket did not have a clear positive effect on implant osseointegration. More studies are needed to clarify the association between local BAF and osseointegration.

In this case report, we aimed to share the clinical and histopathological examination and treatment process of a 6-month-old hybrid cat brought with the complaint of swelling in the medial part of the right auricle. In clinical examination, it was determined that the mass had a hard and irregular surface structure. As a result of the clinical examination, it was decided to completely extirpate the mass and send it to the pathology department. The mass was widely separated from the surrounding tissues and extirpated with the help of cautery. Microscopic examination of the mass revealed that there were chondrocytes among the dense bone cell accumulations and that they formed islands in some areas. As a result of histopathological examinations, it was determined that the suspicious mass was osteochondroma. During the postoperative examinations, it was determined that there was no recurrence or complication in the patient. As a result, it was concluded that this presentation will contribute to the literature since osteochondroma masses are rare in the ear region.

This study aimed to investigate the cytological and biochemical effects of tyrosol on bronchoalveolar lavage fluid (BALF) in an experimental lung injury model induced via intratracheal bleomycin (BLM) administration at 4 mg/kg. Tyrosol is a compound found in olive oil with antioxidant, anti-inflammatory, and antifibrotic activity, and there are no publications on its effect on broncho-alveolar lavage. A total of fifty male Sprague Dawley rats were randomly divided into five groups: control, BLM only, and BLM combined with tyrosol at doses of 20, 40, and 80 mg/kg. Following a two-week treatment period, BALF samples were collected and evaluated cytologically and biochemically. BLM administration led to significant increases in the proportions of lymphocytes, neutrophils, and epithelial cells (p < 0.05) and a decrease in macrophage percentages in BALF. Tyrosol treatment modulated these cellular alterations in a dose-dependent manner, with notable increases in macrophage ratios and reductions in inflammatory cells, particularly at 40 and 80 mg/kg doses. Furthermore, the presence of foamy macrophages—commonly observed in the BLM group—was found to decrease in a dose-dependent manner with tyrosol administration. Biochemical analyses showed that BLM significantly elevated malondialdehyde (MDA) levels (p < 0.05), while reducing the levels of antioxidant enzymes such as superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT). Tyrosol treatment improved these parameters in a dose-dependent manner, thereby reducing oxidative stress. In cytokine analysis, BLM increased all proinflammatory cytokine levels, whereas tyrosol treatment, particularly at higher doses, significantly decreased IL-6 levels (p < 0.05). In conclusion, tyrosol demonstrated notable protective effects against bleomycin-induced lung injury by exerting anti-inflammatory and antioxidative actions at the BALF level.

Dermoid sinus (DS) is a rare congenital cutaneous malformation resulting from incomplete separation of the ectoderm from the neural tube during embryogenesis and is most commonly encountered in dogs along the dorsal midline. Atypical regional occurrences, including cranial localizations, are rare and may pose diagnostic and surgical challenges. This report describes a four-month-old male Pomeranian dog presenting with multiple progressive swellings within the parieto-occipital region of the head. Physical examination revealed firm, poorly demarcated masses without active drainage. Routine hematological and biochemical parameters were unremarkable. Surgical management consisted of complete excision of the lesions followed by reconstructive closure using a random-pattern advancement skin flap due to the extensive skin defect. Histopathological evaluation demonstrated a closed epithelial-lined lesion lacking communication with the epidermal surface, consistent with Type V DS, considering its localization within the parieto-occipital region of the head, the lesion was classified as subtype “b” (Type Vb DS). The postoperative course was uneventful, and no recurrence or wound healing issues were detected during the 12-month follow-up period. To the best of the authors’ knowledge, this represents the first documented report of Type Vb DS in a Pomeranian dog with detailed long-term postoperative follow-up. These findings emphasize the importance of thorough surgical excision and appropriate reconstructive planning when managing extensive DS lesions in atypical cranial regions.