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Manthos Panou
Department of Botany, School of Biology, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece

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Journal article
Published: 17 December 2020 in International Journal of Molecular Sciences
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Microcystins (MCs) are cyanobacterial toxins and potent inhibitors of protein phosphatases 1 (PP1) and 2A (PP2A), which are involved in plant cytoskeleton (microtubules and F-actin) organization. Therefore, studies on the toxicity of cyanobacterial products on plant cells have so far been focused on MCs. In this study, we investigated the effects of extracts from 16 (4 MC-producing and 12 non-MC-producing) cyanobacterial strains from several habitats, on various enzymes (PP1, trypsin, elastase), on the plant cytoskeleton and H2O2 levels in Oryza sativa (rice) root cells. Seedling roots were treated for various time periods (1, 12, and 24 h) with aqueous cyanobacterial extracts and underwent either immunostaining for α-tubulin or staining of F-actin with fluorescent phalloidin. 2,7-dichlorofluorescein diacetate (DCF-DA) staining was performed for H2O2 imaging. The enzyme assays confirmed the bioactivity of the extracts of not only MC-rich (MC+), but also MC-devoid (MC−) extracts, which induced major time-dependent alterations on both components of the plant cytoskeleton. These findings suggest that a broad spectrum of bioactive cyanobacterial compounds, apart from MCs or other known cyanotoxins (such as cylindrospermopsin), can affect plants by disrupting the cytoskeleton.

ACS Style

Dimitris Pappas; Manthos Panou; Ioannis-Dimosthenis S. Adamakis; Spyros Gkelis; Emmanuel Panteris. Beyond Microcystins: Cyanobacterial Extracts Induce Cytoskeletal Alterations in Rice Root Cells. International Journal of Molecular Sciences 2020, 21, 9649 .

AMA Style

Dimitris Pappas, Manthos Panou, Ioannis-Dimosthenis S. Adamakis, Spyros Gkelis, Emmanuel Panteris. Beyond Microcystins: Cyanobacterial Extracts Induce Cytoskeletal Alterations in Rice Root Cells. International Journal of Molecular Sciences. 2020; 21 (24):9649.

Chicago/Turabian Style

Dimitris Pappas; Manthos Panou; Ioannis-Dimosthenis S. Adamakis; Spyros Gkelis; Emmanuel Panteris. 2020. "Beyond Microcystins: Cyanobacterial Extracts Induce Cytoskeletal Alterations in Rice Root Cells." International Journal of Molecular Sciences 21, no. 24: 9649.

Preprint
Published: 26 November 2020
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Microcystins (MCs) are cyanobacterial toxins and potent inhibitors of protein phosphatases 1 (PP1) and 2A (PP2A), which are involved in plant cytoskeleton (microtubules and F-actin) organization. Therefore, studies on the toxicity of cyanobacterial products on plant cells have so far being focused on MCs. In this study, we investigated the effects of extracts from 16 (4 MC-producing and 12 non-MC-producing) cyanobacterial strains from several habitats, on various enzymes (PP1, trypsin, elastase), on the plant cytoskeleton and H2O2 levels in Oryza sativa (rice) root cells. Seedling roots were treated for various time periods (1, 12 and 24h) with aqueous cyanobacterial extracts and underwent either immunostaining for α-tubulin or staining of F-actin with fluorescent phalloidin. DCF-DA staining was performed for H2O2 imaging. The enzyme assays confirmed the bioactivity of the extracts of not only MC-rich (MC+), but also MC-devoid (MC-) extracts, which induced major time-dependent alterations on both components of the plant cytoskeleton. These findings suggest that a broad spectrum of bioactive cyanobacterial compounds, apart from MCs or other known cyanotoxins (such as cylindrospermopsin), can affect plants by disrupting the cytoskeleton.

ACS Style

Dimitris Pappas; Manthos Panou; Ioannis-Dimosthenis S. Adamakis; Spyros Gkelis; Emmanuel Panteris. Beyond Microcystins: Cyanobacterial Extracts Induce Cytoskeletal Alterations in Rice Root Cells. 2020, 1 .

AMA Style

Dimitris Pappas, Manthos Panou, Ioannis-Dimosthenis S. Adamakis, Spyros Gkelis, Emmanuel Panteris. Beyond Microcystins: Cyanobacterial Extracts Induce Cytoskeletal Alterations in Rice Root Cells. . 2020; ():1.

Chicago/Turabian Style

Dimitris Pappas; Manthos Panou; Ioannis-Dimosthenis S. Adamakis; Spyros Gkelis; Emmanuel Panteris. 2020. "Beyond Microcystins: Cyanobacterial Extracts Induce Cytoskeletal Alterations in Rice Root Cells." , no. : 1.

Preprint content
Published: 06 January 2020
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Cyanobacteria have been linked with hydrogen cyanide, based on their ability to catabolize it by the nitrogenase enzyme, as a part of nitrogen fixation. Nitrogenase can also use hydrogen cyanide instead of its normal substrate, dinitrogen and convert it to methane and ammonia. In this study, we tested whether cyanobacteria are able, not only to reduce, but also to produce HCN. The production of HCN was examined in 78 cyanobacteria strains from all five principal sections of cyanobacteria, both non-heterocytous and heterocytous, representing a variety of lifestyles and habitats. Twenty-eight (28) strains were found positive for HCN production, with universal representation amongst 22 cyanobacterial planktic and epilithic genera inhabiting freshwater, brackish, marine (including sponges), and terrestrial (including anchialine) habitats. The HCN production could be linked with nitrogen fixation, as all of HCN producing strains are considered capable of fixing nitrogen. Epilithic lifestyle, where cyanobacteria are more vulnerable to a number of grazers and accumulate more glycine, had the largest percentage (75%) of HCN-producing cyanobacteria compared to strains from aquatic ecosystems. Further, we demonstrate the isolation and characterisation of taxa likeGeitleria calcareaandKovacikia muscicola, for which no strain existed andChlorogloeasp. TAU-MAC 0618 which is, to the best of our knowledge, the first bacterium isolate from anchialine ecosystems. Our results highlight the complexity of cyanobacteria secondary metabolism, as well as the diversity of cyanobacteria in underexplored habitats, providing a missing study material for this type of environments.

ACS Style

Manthos Panou; Spyros Gkelis. Cyano-assassins: Widespread cyanogenic production from cyanobacteria. 2020, 1 .

AMA Style

Manthos Panou, Spyros Gkelis. Cyano-assassins: Widespread cyanogenic production from cyanobacteria. . 2020; ():1.

Chicago/Turabian Style

Manthos Panou; Spyros Gkelis. 2020. "Cyano-assassins: Widespread cyanogenic production from cyanobacteria." , no. : 1.

Journal article
Published: 25 July 2019 in Toxins
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Cyanobacteria are a diverse group of photosynthetic Gram-negative bacteria that produce an array of secondary compounds with selective bioactivity against a broad spectrum of organisms and cell lines. In this study, 29 strains isolated from freshwaters in Greece were classified using a polyphasic approach and assigned to Chroococcales, Synechococcales, and Nostocales, representing 11 genera and 17 taxa. There were good agreements between 16S ribosomal RNA (rRNA)–cpcBA–internal genetic spacer (IGS) characterization and morphological features, except for the Jaaginema–Limnothrix group which appears intermixed and needs further elucidation. Methanol extracts of the strains were analyzed for cyanotoxin production and tested against pathogenic bacteria species and several cancer cell lines. We report for the first time a Nostoc oryzae strain isolated from rice fields capable of producing microcystins (MCs) and a Chlorogloeopsis fritschii strain isolated from the plankton of a lake, suggesting that this species may also occur in freshwater temperate habitats. Strains with very high or identical 16S rRNA gene sequences displayed different antibacterial and cytotoxic activities. Extracts from Synechococcus cf. nidulans showed the most potent antibacterial activity against Staphylococcus aureus, whereas Jaaginema sp. strains exhibited potent cytotoxic activities against human colorectal adenocarcinoma and hepatocellular carcinoma cells. Jaaginema Thessaloniki Aristotle University Microalgae and Cyanobacteria (TAU-MAC) 0110 and 0210 strains caused pronounced changes in the actin network and triggered the formation of numerous lipid droplets in hepatocellular carcinoma and green monkey kidney cells, suggesting oxidative stress and/or mitochondrial damage leading to apoptosis.

ACS Style

Spyros Gkelis; Manthos Panou; Despoina Konstantinou; Panagiotis Apostolidis; Antonia Kasampali; Sofia Papadimitriou; Dominiki Kati; Giorgia Maria Di Lorenzo; Stamatia Ioakeim; Sevasti-Kiriaki Zervou; Christophoros Christophoridis; Theodoros M. Triantis; Triantafyllos Kaloudis; Anastasia Hiskia; Minas Arsenakis. Diversity, Cyanotoxin Production, and Bioactivities of Cyanobacteria Isolated from Freshwaters of Greece. Toxins 2019, 11, 436 .

AMA Style

Spyros Gkelis, Manthos Panou, Despoina Konstantinou, Panagiotis Apostolidis, Antonia Kasampali, Sofia Papadimitriou, Dominiki Kati, Giorgia Maria Di Lorenzo, Stamatia Ioakeim, Sevasti-Kiriaki Zervou, Christophoros Christophoridis, Theodoros M. Triantis, Triantafyllos Kaloudis, Anastasia Hiskia, Minas Arsenakis. Diversity, Cyanotoxin Production, and Bioactivities of Cyanobacteria Isolated from Freshwaters of Greece. Toxins. 2019; 11 (8):436.

Chicago/Turabian Style

Spyros Gkelis; Manthos Panou; Despoina Konstantinou; Panagiotis Apostolidis; Antonia Kasampali; Sofia Papadimitriou; Dominiki Kati; Giorgia Maria Di Lorenzo; Stamatia Ioakeim; Sevasti-Kiriaki Zervou; Christophoros Christophoridis; Theodoros M. Triantis; Triantafyllos Kaloudis; Anastasia Hiskia; Minas Arsenakis. 2019. "Diversity, Cyanotoxin Production, and Bioactivities of Cyanobacteria Isolated from Freshwaters of Greece." Toxins 11, no. 8: 436.

Journal article
Published: 15 October 2018 in Harmful Algae
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The cyanobacterium Cylindrospermopsis raciborskii represents a challenge for researchers and it is extensively studied for its toxicity and invasive behaviour, which is presumably enhanced by global warming. Biogeography studies indicate a tropical origin for this species, with Greece considered as the expansion route of C. raciborskii in Europe. The widening of its geographic distribution and the isolation of strains showing high optimum growth temperature underline its ecological heterogeneity, suggesting the existence of different ecotypes. The dominance of species like C. raciborskii along with their ecotoxicology and potential human risk related problems, render the establishment of a clear phylogeography model essential. In the context of the present study, the characterization of Cylindrospermopsis raciborskii TAU-MAC 1414 strain, isolated from Lake Karla, with respect to its phylogeography and toxic potential, is attempted. Our research provides new insights on the origin of C. raciborskii in the Mediterranean region; C. raciborskii expanded in Mediterranean from North America, whilst the rest of the European strains may originate from Asia and Australia. Microcystin synthetase genes, phylogenetic closely related with Microcystis strains, were also present in C. raciborskii TAU-MAC 1414. We were unable to unambiguously confirm the presence of MC-LR, using LC–MS/MS. Our results are shedding light on the expansion and distribution of C. raciborskii, whilst they pose further questions on the toxic capacity of this species.

ACS Style

Manthos Panou; Sevasti-Kiriaki Zervou; Triantafyllos Kaloudis; Anastasia Hiskia; Spyros Gkelis. A Greek Cylindrospermopsis raciborskii strain: Missing link in tropic invader’s phylogeography tale. Harmful Algae 2018, 80, 96 -106.

AMA Style

Manthos Panou, Sevasti-Kiriaki Zervou, Triantafyllos Kaloudis, Anastasia Hiskia, Spyros Gkelis. A Greek Cylindrospermopsis raciborskii strain: Missing link in tropic invader’s phylogeography tale. Harmful Algae. 2018; 80 ():96-106.

Chicago/Turabian Style

Manthos Panou; Sevasti-Kiriaki Zervou; Triantafyllos Kaloudis; Anastasia Hiskia; Spyros Gkelis. 2018. "A Greek Cylindrospermopsis raciborskii strain: Missing link in tropic invader’s phylogeography tale." Harmful Algae 80, no. : 96-106.

Journal article
Published: 13 April 2018 in Toxins
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Insight into how environmental change determines the production and distribution of cyanobacterial toxins is necessary for risk assessment. Management guidelines currently focus on hepatotoxins (microcystins). Increasing attention is given to other classes, such as neurotoxins (e.g., anatoxin-a) and cytotoxins (e.g., cylindrospermopsin) due to their potency. Most studies examine the relationship between individual toxin variants and environmental factors, such as nutrients, temperature and light. In summer 2015, we collected samples across Europe to investigate the effect of nutrient and temperature gradients on the variability of toxin production at a continental scale. Direct and indirect effects of temperature were the main drivers of the spatial distribution in the toxins produced by the cyanobacterial community, the toxin concentrations and toxin quota. Generalized linear models showed that a Toxin Diversity Index (TDI) increased with latitude, while it decreased with water stability. Increases in TDI were explained through a significant increase in toxin variants such as MC-YR, anatoxin and cylindrospermopsin, accompanied by a decreasing presence of MC-LR. While global warming continues, the direct and indirect effects of increased lake temperatures will drive changes in the distribution of cyanobacterial toxins in Europe, potentially promoting selection of a few highly toxic species or strains.

ACS Style

Evanthia Mantzouki; Miquel Lürling; Jutta Fastner; Lisette De Senerpont Domis; Elżbieta Wilk-Woźniak; Judita Koreivienė; Laura Seelen; Sven Teurlincx; Yvon Verstijnen; Wojciech Krztoń; Edward Walusiak; Jūratė Karosienė; Jūratė Kasperovičienė; Ksenija Savadova; Irma Vitonytė; Carmen Cillero-Castro; Agnieszka Budzyńska; Ryszard Goldyn; Anna Kozak; Joanna Rosińska; Elżbieta Szeląg-Wasielewska; Piotr Domek; Natalia Jakubowska-Krepska; Kinga Kwasizur; Beata Messyasz; Aleksandra Pełechata; Mariusz Pełechaty; Mikolaj Kokocinski; Ana García-Murcia; Monserrat Real; Elvira Romans; Jordi Noguero-Ribes; David Parreño Duque; Elísabeth Fernández-Morán; Nusret Karakaya; Kerstin Häggqvist; Nilsun Demir; Meryem Beklioğlu; Nur Filiz; Eti E. Levi; Uğur Iskin; Gizem Bezirci; Ülkü Nihan Tavşanoğlu; Koray Özhan; Spyros Gkelis; Manthos Panou; Özden Fakioglu; Christos Avagianos; Triantafyllos Kaloudis; Kemal Çelik; Mete Yilmaz; Rafael Marcé; Nuria Catalán; Andrea G. Bravo; Moritz Buck; William Colom-Montero; Kristiina Mustonen; Don Pierson; Yang Yang; Pedro M. Raposeiro; Vítor Gonçalves; Maria G. Antoniou; Nikoletta Tsiarta; Valerie McCarthy; Victor C. Perello; Tõnu Feldmann; Alo Laas; Kristel Panksep; Lea Tuvikene; Ilona Gagala; Joana Mankiewicz-Boczek; Meral Apaydın Yağcı; Şakir Çınar; Kadir Çapkın; Abdulkadir Yağcı; Mehmet Cesur; Fuat Bilgin; Cafer Bulut; Rahmi Uysal; Ulrike Obertegger; Adriano Boscaini; Giovanna Flaim; Nico Salmaso; Leonardo Cerasino; Jessica Richardson; Petra M. Visser; Jolanda M. H. Verspagen; Tünay Karan; Elif Neyran Soylu; Faruk Maraşlıoğlu; Agnieszka Napiórkowska-Krzebietke; Agnieszka Ochocka; Agnieszka Pasztaleniec; Ana M. Antão-Geraldes; Vitor Vasconcelos; João Morais; Micaela Vale; Latife Köker; Reyhan Akçaalan; Meriç Albay; Dubravka Špoljarić Maronić; Filip Stević; Tanja Žuna Pfeiffer; Jeremy Fonvielle; Dietmar Straile; Karl-Otto Rothhaupt; Lars-Anders Hansson; Pablo Urrutia-Cordero; Luděk Bláha; Rodan Geriš; Markéta Fránková; Mehmet Ali Turan Koçer; Mehmet Tahir Alp; Spela Remec-Rekar; Tina Elersek; Theodoros Triantis; Sevasti-Kiriaki Zervou; Anastasia Hiskia; Sigrid Haande; Birger Skjelbred; Beata Madrecka; Hana Nemova; Iveta Drastichova; Lucia Chomova; Christine Edwards; Tuğba Ongun Sevindik; Hatice Tunca; Burçin Önem; Boris Aleksovski; Svetislav Krstić; Itana Bokan Vucelić; Lidia Nawrocka; Pauliina Salmi; Danielle Machado-Vieira; Alinne Gurjão De Oliveira; Jordi Delgado-Martín; David García; Jose Luís Cereijo; Joan Gomà; Mari Carmen Trapote; Teresa Vegas-Vilarrúbia; Biel Obrador; Magdalena Grabowska; Maciej Karpowicz; Damian Chmura; Bárbara Úbeda; José Ángel Gálvez; Arda Özen; Kirsten Seestern Christoffersen; Trine Perlt Warming; Justyna Kobos; Hanna Mazur-Marzec; Carmen Pérez-Martínez; Eloísa Ramos-Rodríguez; Lauri Arvola; Pablo Alcaraz-Párraga; Magdalena Toporowska; Barbara Pawlik-Skowronska; Michał Niedźwiecki; Wojciech Pęczuła; Manel Leira; Armand Hernández; Enrique Moreno-Ostos; José María Blanco; Valeriano Rodríguez; Jorge Juan Montes-Pérez; Roberto L. Palomino; Estela Rodríguez-Pérez; Rafael Carballeira; Antonio Camacho; Antonio Picazo; Carlos Rochera; Anna C. Santamans; Carmen Ferriol; Susana Romo; Juan Miguel Soria; Julita Dunalska; Justyna Sieńska; Daniel Szymański; Marek Kruk; Iwona Kostrzewska-Szlakowska; Iwona Jasser; Petar Žutinić; Marija Gligora Udovič; Anđelka Plenković-Moraj; Magdalena Frąk; Agnieszka Bańkowska-Sobczak; Michał Wasilewicz; Korhan Özkan; Valentini Maliaka; Kersti Kangro; Hans-Peter Grossart; Hans Paerl; Cayelan C. Carey; Bas W. Ibelings. Temperature Effects Explain Continental Scale Distribution of Cyanobacterial Toxins. Toxins 2018, 10, 156 .

AMA Style

Evanthia Mantzouki, Miquel Lürling, Jutta Fastner, Lisette De Senerpont Domis, Elżbieta Wilk-Woźniak, Judita Koreivienė, Laura Seelen, Sven Teurlincx, Yvon Verstijnen, Wojciech Krztoń, Edward Walusiak, Jūratė Karosienė, Jūratė Kasperovičienė, Ksenija Savadova, Irma Vitonytė, Carmen Cillero-Castro, Agnieszka Budzyńska, Ryszard Goldyn, Anna Kozak, Joanna Rosińska, Elżbieta Szeląg-Wasielewska, Piotr Domek, Natalia Jakubowska-Krepska, Kinga Kwasizur, Beata Messyasz, Aleksandra Pełechata, Mariusz Pełechaty, Mikolaj Kokocinski, Ana García-Murcia, Monserrat Real, Elvira Romans, Jordi Noguero-Ribes, David Parreño Duque, Elísabeth Fernández-Morán, Nusret Karakaya, Kerstin Häggqvist, Nilsun Demir, Meryem Beklioğlu, Nur Filiz, Eti E. Levi, Uğur Iskin, Gizem Bezirci, Ülkü Nihan Tavşanoğlu, Koray Özhan, Spyros Gkelis, Manthos Panou, Özden Fakioglu, Christos Avagianos, Triantafyllos Kaloudis, Kemal Çelik, Mete Yilmaz, Rafael Marcé, Nuria Catalán, Andrea G. Bravo, Moritz Buck, William Colom-Montero, Kristiina Mustonen, Don Pierson, Yang Yang, Pedro M. Raposeiro, Vítor Gonçalves, Maria G. Antoniou, Nikoletta Tsiarta, Valerie McCarthy, Victor C. Perello, Tõnu Feldmann, Alo Laas, Kristel Panksep, Lea Tuvikene, Ilona Gagala, Joana Mankiewicz-Boczek, Meral Apaydın Yağcı, Şakir Çınar, Kadir Çapkın, Abdulkadir Yağcı, Mehmet Cesur, Fuat Bilgin, Cafer Bulut, Rahmi Uysal, Ulrike Obertegger, Adriano Boscaini, Giovanna Flaim, Nico Salmaso, Leonardo Cerasino, Jessica Richardson, Petra M. Visser, Jolanda M. H. Verspagen, Tünay Karan, Elif Neyran Soylu, Faruk Maraşlıoğlu, Agnieszka Napiórkowska-Krzebietke, Agnieszka Ochocka, Agnieszka Pasztaleniec, Ana M. Antão-Geraldes, Vitor Vasconcelos, João Morais, Micaela Vale, Latife Köker, Reyhan Akçaalan, Meriç Albay, Dubravka Špoljarić Maronić, Filip Stević, Tanja Žuna Pfeiffer, Jeremy Fonvielle, Dietmar Straile, Karl-Otto Rothhaupt, Lars-Anders Hansson, Pablo Urrutia-Cordero, Luděk Bláha, Rodan Geriš, Markéta Fránková, Mehmet Ali Turan Koçer, Mehmet Tahir Alp, Spela Remec-Rekar, Tina Elersek, Theodoros Triantis, Sevasti-Kiriaki Zervou, Anastasia Hiskia, Sigrid Haande, Birger Skjelbred, Beata Madrecka, Hana Nemova, Iveta Drastichova, Lucia Chomova, Christine Edwards, Tuğba Ongun Sevindik, Hatice Tunca, Burçin Önem, Boris Aleksovski, Svetislav Krstić, Itana Bokan Vucelić, Lidia Nawrocka, Pauliina Salmi, Danielle Machado-Vieira, Alinne Gurjão De Oliveira, Jordi Delgado-Martín, David García, Jose Luís Cereijo, Joan Gomà, Mari Carmen Trapote, Teresa Vegas-Vilarrúbia, Biel Obrador, Magdalena Grabowska, Maciej Karpowicz, Damian Chmura, Bárbara Úbeda, José Ángel Gálvez, Arda Özen, Kirsten Seestern Christoffersen, Trine Perlt Warming, Justyna Kobos, Hanna Mazur-Marzec, Carmen Pérez-Martínez, Eloísa Ramos-Rodríguez, Lauri Arvola, Pablo Alcaraz-Párraga, Magdalena Toporowska, Barbara Pawlik-Skowronska, Michał Niedźwiecki, Wojciech Pęczuła, Manel Leira, Armand Hernández, Enrique Moreno-Ostos, José María Blanco, Valeriano Rodríguez, Jorge Juan Montes-Pérez, Roberto L. Palomino, Estela Rodríguez-Pérez, Rafael Carballeira, Antonio Camacho, Antonio Picazo, Carlos Rochera, Anna C. Santamans, Carmen Ferriol, Susana Romo, Juan Miguel Soria, Julita Dunalska, Justyna Sieńska, Daniel Szymański, Marek Kruk, Iwona Kostrzewska-Szlakowska, Iwona Jasser, Petar Žutinić, Marija Gligora Udovič, Anđelka Plenković-Moraj, Magdalena Frąk, Agnieszka Bańkowska-Sobczak, Michał Wasilewicz, Korhan Özkan, Valentini Maliaka, Kersti Kangro, Hans-Peter Grossart, Hans Paerl, Cayelan C. Carey, Bas W. Ibelings. Temperature Effects Explain Continental Scale Distribution of Cyanobacterial Toxins. Toxins. 2018; 10 (4):156.

Chicago/Turabian Style

Evanthia Mantzouki; Miquel Lürling; Jutta Fastner; Lisette De Senerpont Domis; Elżbieta Wilk-Woźniak; Judita Koreivienė; Laura Seelen; Sven Teurlincx; Yvon Verstijnen; Wojciech Krztoń; Edward Walusiak; Jūratė Karosienė; Jūratė Kasperovičienė; Ksenija Savadova; Irma Vitonytė; Carmen Cillero-Castro; Agnieszka Budzyńska; Ryszard Goldyn; Anna Kozak; Joanna Rosińska; Elżbieta Szeląg-Wasielewska; Piotr Domek; Natalia Jakubowska-Krepska; Kinga Kwasizur; Beata Messyasz; Aleksandra Pełechata; Mariusz Pełechaty; Mikolaj Kokocinski; Ana García-Murcia; Monserrat Real; Elvira Romans; Jordi Noguero-Ribes; David Parreño Duque; Elísabeth Fernández-Morán; Nusret Karakaya; Kerstin Häggqvist; Nilsun Demir; Meryem Beklioğlu; Nur Filiz; Eti E. Levi; Uğur Iskin; Gizem Bezirci; Ülkü Nihan Tavşanoğlu; Koray Özhan; Spyros Gkelis; Manthos Panou; Özden Fakioglu; Christos Avagianos; Triantafyllos Kaloudis; Kemal Çelik; Mete Yilmaz; Rafael Marcé; Nuria Catalán; Andrea G. Bravo; Moritz Buck; William Colom-Montero; Kristiina Mustonen; Don Pierson; Yang Yang; Pedro M. Raposeiro; Vítor Gonçalves; Maria G. Antoniou; Nikoletta Tsiarta; Valerie McCarthy; Victor C. Perello; Tõnu Feldmann; Alo Laas; Kristel Panksep; Lea Tuvikene; Ilona Gagala; Joana Mankiewicz-Boczek; Meral Apaydın Yağcı; Şakir Çınar; Kadir Çapkın; Abdulkadir Yağcı; Mehmet Cesur; Fuat Bilgin; Cafer Bulut; Rahmi Uysal; Ulrike Obertegger; Adriano Boscaini; Giovanna Flaim; Nico Salmaso; Leonardo Cerasino; Jessica Richardson; Petra M. Visser; Jolanda M. H. Verspagen; Tünay Karan; Elif Neyran Soylu; Faruk Maraşlıoğlu; Agnieszka Napiórkowska-Krzebietke; Agnieszka Ochocka; Agnieszka Pasztaleniec; Ana M. Antão-Geraldes; Vitor Vasconcelos; João Morais; Micaela Vale; Latife Köker; Reyhan Akçaalan; Meriç Albay; Dubravka Špoljarić Maronić; Filip Stević; Tanja Žuna Pfeiffer; Jeremy Fonvielle; Dietmar Straile; Karl-Otto Rothhaupt; Lars-Anders Hansson; Pablo Urrutia-Cordero; Luděk Bláha; Rodan Geriš; Markéta Fránková; Mehmet Ali Turan Koçer; Mehmet Tahir Alp; Spela Remec-Rekar; Tina Elersek; Theodoros Triantis; Sevasti-Kiriaki Zervou; Anastasia Hiskia; Sigrid Haande; Birger Skjelbred; Beata Madrecka; Hana Nemova; Iveta Drastichova; Lucia Chomova; Christine Edwards; Tuğba Ongun Sevindik; Hatice Tunca; Burçin Önem; Boris Aleksovski; Svetislav Krstić; Itana Bokan Vucelić; Lidia Nawrocka; Pauliina Salmi; Danielle Machado-Vieira; Alinne Gurjão De Oliveira; Jordi Delgado-Martín; David García; Jose Luís Cereijo; Joan Gomà; Mari Carmen Trapote; Teresa Vegas-Vilarrúbia; Biel Obrador; Magdalena Grabowska; Maciej Karpowicz; Damian Chmura; Bárbara Úbeda; José Ángel Gálvez; Arda Özen; Kirsten Seestern Christoffersen; Trine Perlt Warming; Justyna Kobos; Hanna Mazur-Marzec; Carmen Pérez-Martínez; Eloísa Ramos-Rodríguez; Lauri Arvola; Pablo Alcaraz-Párraga; Magdalena Toporowska; Barbara Pawlik-Skowronska; Michał Niedźwiecki; Wojciech Pęczuła; Manel Leira; Armand Hernández; Enrique Moreno-Ostos; José María Blanco; Valeriano Rodríguez; Jorge Juan Montes-Pérez; Roberto L. Palomino; Estela Rodríguez-Pérez; Rafael Carballeira; Antonio Camacho; Antonio Picazo; Carlos Rochera; Anna C. Santamans; Carmen Ferriol; Susana Romo; Juan Miguel Soria; Julita Dunalska; Justyna Sieńska; Daniel Szymański; Marek Kruk; Iwona Kostrzewska-Szlakowska; Iwona Jasser; Petar Žutinić; Marija Gligora Udovič; Anđelka Plenković-Moraj; Magdalena Frąk; Agnieszka Bańkowska-Sobczak; Michał Wasilewicz; Korhan Özkan; Valentini Maliaka; Kersti Kangro; Hans-Peter Grossart; Hans Paerl; Cayelan C. Carey; Bas W. Ibelings. 2018. "Temperature Effects Explain Continental Scale Distribution of Cyanobacterial Toxins." Toxins 10, no. 4: 156.

Journal article
Published: 03 May 2017 in Advances in Oceanography and Limnology
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Lake Karla (Central Greece) is a unique example - at European scale - of a shallow lake ecosystem that was dried in the 1960s and in 2009 started to be restored. The lake is listed in the network of the Greek protected areas as it is considered a vital aquatic ecosystem, in terms of biodiversity. It has, however, already been adversely affected by both agricultural and industrial land uses in the surrounding area, leading to eutrophication and shifting algal community towards bloom-forming toxic cyanobacterial species. After repeated heavy-blooms, cyanotoxin occurrence and mass fish kills, the local ecosystem management authority has implemented a water quality monitoring program (July 2013 - July 2015) to assess environmental pressures and the response of aquatic biota in the lake. Microscopic, immunological, and molecular techniques combined with physico-chemical parameters, complemented by liquid chromatography tandem mass spectrometry (LC-MS/MS), were used to monitor cyanobacteria blooms and the associated cyanotoxin production from three different sites in Lake Karla and from the adjacent Kalamaki Reservoir. Water quality was also assessed by the structure of benthic invertebrate community on the sediment. Cyanobacteria were the main phytoplankton component, representing more than 70% of the total phytoplankton abundance; dominant taxa belonged to Cylindrospermopsis raciborskii, Limnothrix redekei, Anabaenopsis elenkinii, and Microcystis spp. Euglenophytes (Euglena), diatoms (Nitzschia), and chlorophytes (Scenedesmus) were also important phytoplankton constituents. LC-MS/MS confirmed the co-occurrence of microcystins, cylindrospermopsin, saxitoxin, neo-saxitoxin and anatoxin-a. The occurrence of cyanotoxins in relation to the persistent and dominant cyanobacteria and the impact of cyanobacterial harmful algal blooms on the newly constructed lake along with the land uses and the emergent mitigation measures are discussed.

ACS Style

Spyros Gkelis; Manthos Panou; Ioannis Chronis; Sevasti-Kiriaki Zervou; Christophoros Christophoridis; Korina Manolidi; Chrysoula Ntislidou; Theodoros M. Triantis; Triantafyllos Kaloudis; Anastasia Hiskia; Ifigenia Kagalou; Maria Lazaridou. Monitoring a newly re-born patient: water quality and cyanotoxin occurrence in a reconstructed shallow Mediterranean lake. Advances in Oceanography and Limnology 2017, 8, 1 .

AMA Style

Spyros Gkelis, Manthos Panou, Ioannis Chronis, Sevasti-Kiriaki Zervou, Christophoros Christophoridis, Korina Manolidi, Chrysoula Ntislidou, Theodoros M. Triantis, Triantafyllos Kaloudis, Anastasia Hiskia, Ifigenia Kagalou, Maria Lazaridou. Monitoring a newly re-born patient: water quality and cyanotoxin occurrence in a reconstructed shallow Mediterranean lake. Advances in Oceanography and Limnology. 2017; 8 (1):1.

Chicago/Turabian Style

Spyros Gkelis; Manthos Panou; Ioannis Chronis; Sevasti-Kiriaki Zervou; Christophoros Christophoridis; Korina Manolidi; Chrysoula Ntislidou; Theodoros M. Triantis; Triantafyllos Kaloudis; Anastasia Hiskia; Ifigenia Kagalou; Maria Lazaridou. 2017. "Monitoring a newly re-born patient: water quality and cyanotoxin occurrence in a reconstructed shallow Mediterranean lake." Advances in Oceanography and Limnology 8, no. 1: 1.

Journal article
Published: 01 November 2016 in Biodiversity Data Journal
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The checklist of Greek Cyanobacteria comprises 543 species, classified in 130 genera, 41 families, and 8 orders. The orders Synechococcales and Oscillatoriales have the highest number of species (158 and 153 species, respectively), whereas these two orders along with Nostocales and Chroococcales cover 93% of the known Greek cyanobacteria species. It is worth mentioning that 18 species have been initially described from Greek habitats. The marine epilithic Ammatoidea aegea described from Saronikos Gulf is considered endemic to this area. Our bibliographic review shows that Greece hosts a high diversity of cyanobacteria, suggesting that the Mediterranean area is also a hot spot for microbes. The checklist of Greek Cyanobacteria comprises 543 species, classified in 130 genera, 41 families, and 8 orders. The orders Synechococcales and Oscillatoriales have the highest number of species (158 and 153 species, respectively), whereas these two orders along with Nostocales and Chroococcales cover 93% of the known Greek cyanobacteria species. It is worth mentioning that 18 species have been initially described from Greek habitats. The marine epilithic Ammatoidea aegea described from Saronikos Gulf is considered endemic to this area. Our bibliographic review shows that Greece hosts a high diversity of cyanobacteria, suggesting that the Mediterranean area is also a hot spot for microbes.

ACS Style

Spyros Gkelis; Iordanis Ourailidis; Manthos Panou; Nikos Pappas. Cyanobacteria of Greece: an annotated checklist. Biodiversity Data Journal 2016, 4, e10084 .

AMA Style

Spyros Gkelis, Iordanis Ourailidis, Manthos Panou, Nikos Pappas. Cyanobacteria of Greece: an annotated checklist. Biodiversity Data Journal. 2016; 4 (4):e10084.

Chicago/Turabian Style

Spyros Gkelis; Iordanis Ourailidis; Manthos Panou; Nikos Pappas. 2016. "Cyanobacteria of Greece: an annotated checklist." Biodiversity Data Journal 4, no. 4: e10084.

Journal article
Published: 06 April 2016 in Biodiversity Data Journal
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Currently, cyanobacterial diversity is examined using a polyphasic approach by assessing morphological and molecular data (Komárek 2015). However, the comparison of morphological and genetic data is sometimes hindered by the lack of cultures of several cyanobacterial morphospecies and inadequate morphological data of sequenced strains (Rajaniemi et al. 2005). Furthermore, in order to evaluate the phenotypic plasticity within defined taxa, the variability observed in cultures has to be compared to the range in natural variation (Komárek and Mareš 2012). Thus, new tools are needed to aggregate, link and process data in a meaningful way, in order to properly study and understand cyanodiversity. An online database on cyanobacteria has been created, namely the Cyanobacteria culture collection (CCC) (http://cyanobacteria.myspecies.info/) using as case studies cyanobacterial strains isolated from lakes of Greece, which are part of the AUTH culture collection (School of Biology, Aristotle University of Thessaloniki). The database hosts, for the first time, information and data such as morphology/morphometry, biogeography, phylogeny, microphotographs, distribution maps, toxicology and biochemical traits of the strains. All this data are structured managed, and presented online and are publicly accessible with a recently developed tool, namely “Scratchpads”, a taxon-centric virtual research environment allowing browsing the taxonomic classification and retrieving various kinds of relevant information for each taxon.

ACS Style

Spyros Gkelis; Manthos Panou. Capturing biodiversity: linking a cyanobacteria culture collection to the “scratchpads” virtual research environment enhances biodiversity knowledge. Biodiversity Data Journal 2016, 4, e7965 -1.

AMA Style

Spyros Gkelis, Manthos Panou. Capturing biodiversity: linking a cyanobacteria culture collection to the “scratchpads” virtual research environment enhances biodiversity knowledge. Biodiversity Data Journal. 2016; 4 (4):e7965-1.

Chicago/Turabian Style

Spyros Gkelis; Manthos Panou. 2016. "Capturing biodiversity: linking a cyanobacteria culture collection to the “scratchpads” virtual research environment enhances biodiversity knowledge." Biodiversity Data Journal 4, no. 4: e7965-1.