This study assessed the efficacy of five denture cleansers on the microbial adherence and surface topography of conventional and CAD/CAM denture base resins. Acrylic resin discs were fabricated using conventional, milling, and 3D printing methods (N = 180). The discs were contaminated with dual species of Candida albicans and Streptococcus mutans biofilm for 72 h and then disinfected with either of the denture cleansers (Fittydent cleansing tablets, 2% Chlorhexidine gluconate, 0.2% Chlorhexidine gluconate, 0.5% sodium hypochlorite, and 1% sodium hypochlorite (n = 10). Distilled water served as the control group. The colony-forming units of the microorganisms were calculated, followed by post-treatment surface roughness. Data were statistically analyzed using one-way ANOVA, paired t-test, and post hoc Tukey HSD test (α = 0.05). Among the denture cleansers, 2% Chlorhexidine gluconate, 0.5% sodium hypochlorite, and 1% sodium hypochlorite had the best cleansing effect on the resin discs and demonstrated zero growth of colonies for both the species. Comparing the material groups, the 3D-processed discs showed higher colony-forming units followed by the conventional and CAD/CAM milled group. The highest surface roughness was demonstrated by the 3D-printed discs (0.690 ± 0.08 μm), followed by the conventional (0.493 ± 0.11 μm) and the milled groups (0.301 ± 0.08 μm). The tested chemical denture cleansers affected the Candida albicans and Streptococcus mutans adhesion compared to control discs immersed in distilled water. The clinician may recommend to their patient to use 2% chlorhexidine gluconate for the disinfection of CAD/CAM PMMA denture base materials.

Background/Objectives: The aim of this study was to compare the outcomes of pulpotomy done in primary molars under different treatment settings, namely: Basic Behavior Guidance (BBG), Oral Sedation (OS) and General Anesthesia (GA). Methods: In this is retrospective study, dental records of 79 pediatric dental patients treated in university dental hospital between March 2019 to January 2022 were reviewed. Data collected include: (1) age at the time of receiving the treatment, (2) age at the recall visit, (3) gender, and (4) type of clinical setting when providing the dental treatment (BBG, OS, or GA). A total of 154 primary molars received pulpotomy procedure. Recall visits include clinical and radiographic examination. Results: Clinically, six teeth in the BBG group exfoliated. SSC was lost in all groups, but no significant difference was found (P= 0.236). For the percussion and palpation responses, no significant differences were found between the three groups (p= 0.245 and 0.395 respectively). Radiographic findings showed no significant difference between the three groups when roots and pulp canals and bone resorption were examined (p= 0.313 and 0.193 respectively). Signiant differences were found when dentinal bridge formation was assessed (p=0.003). When the quality of the pulpotomy, success of the SSC, and the success of the pulpotomy were compared, no significant differences were found (P>0.05). Conclusions: No significant differences were found between the three groups when the pulpotomy outcomes were assessed. This retrospective study highlights the effect of treatment settings. Future prospective study is recommended.