The electrical conductivity of soil saturated paste extract (EC_e) is used as an indicator for estimating soil salinity. This method is time consuming and laborious and therefore, easier and faster methods are usually used with different soil/water ratios, such as 1:5 (EC_1:5), for estimating the EC_e. Usually, the relationship between EC_e and EC_1:5 is described by a simple linear empirical equation. The value of the conversion factor (CF) of EC_1:5 to EC_e is affected by the particular characteristics of the soil, such as its texture. The objective of this study is to investigate models that allow the inclusion of soil texture in the calculation of the CF, in order to improve the prediction of the EC_e. A total of 148 soil samples with different soil texture and salinity levels were selected from three regions of Greece, and EC_e, EC_1:5, as well as clay and sand contents were determined. The results show that the CF can be estimated from an equation which incorporates the clay and sand contents through the soil saturation percentage (SP) and can give a fairly good prediction of EC_e from EC_1:5 (R² = 0.9887 and RMSE = 1.39 dSm⁻¹).

The aim of the present study, conducted under the ERA-NET ARIMNet2 Project (Agricultural Research in the Mediterranean Network), was to analyze the sheep and goat dairy sector and assess the quality and safety of milk at various stages of the dairy supply chain. The study took place in the north-west part of Greece; 52 farms, one milk collection center, and eight dairy processing companies participated. The results revealed that all farms had access to water and electricity, the majority of them had a license to operate, used non-organic milk, and employed family members. The price of milk was fairly unstable throughout the year. Two major dairy companies produced all year round while others seasonally. Products were sold in retail, wholesale, company-owned stores, and even by door-to-door delivery service. The analyzed milk samples were of good quality and safety. The strengths, weaknesses, opportunities, and threats (SWOT) analysis revealed the farmers’ determination to continue working in the sector despite milk prices and problems due to the shortage of cash. Local sheep and goat breeds may further be used due to the fact of their good adaptation to the environmental conditions. The future of the Greek dairy chain is promising despite high competition within and outside of the European Union.

The inclusion of feed additives and the implementation of various nutritional strategies are studied to modify the rumen microbiome and consequently its function. Nevertheless, rumen enzymatic activity and its intermediate products are not always matched with the microbiome structure. To further elucidate such differences a two-phase trial using twenty-two dairy goats was carried out. During the first phase, both groups (20HF n = 11; high forage and 20HG n = 11; high grain) were supplemented with 20 g Schizochytrium spp./goat/day. The 20HF group consumed a diet with a forage:concentrate (F:C) ratio of 60:40 and the 20HG-diet consisted of a F:C = 40:60. In the second phase, the supplementation level of Schizochytrium spp. was increased to 40 g/day/goat while the F:C ratio between the two groups were remained identical (40HF n = 11; high forage and 40HG n = 11; high grain). By utilizing a next-generation sequencing technology, we monitored that the high microalgae inclusion level and foremost in combination with a high grains diet increased the unmapped bacteria within the rumen. Bacteroidetes and Prevotella brevis were increased in the 40HG -fed goats as observed by using a qPCR platform. Additionally, methanogens and Methanomassiliicoccales were increased in high microalgae-fed goats, while Methanobrevibacter and Methanobacteriales were decreased. Fibrolytic bacteria were decreased in high microalgae-fed goats, while cellulolytic activity was increased. Ammonia was decreased in high grains-fed goats, while docosapentaenoic and docosahexaenoic acids showed a lower degradation rate in the rumen of high forage-fed goats. The alteration of the F:C ratio in goats supplemented with Schizochytrium spp. levels modified both ruminal microbiota and enzymatic activity. However, there was no significant consistency in the relations between them.

This study aimed to determine the impact of sesame meal, selenium (Se), and vitamin E (VitE) on goats’ oxidative status. Thirty mid-lactation crossbred goats were divided into five homogeneous groups, and were fed 1 kg of alfalfa hay and 1.2 kg of concentrates daily. The control group (C) received a basal diet. In the concentrates of the treated groups, 10% of the soybean meal was replaced by sesame meal and no extra VitE or Se (SM), or an extra 60 mg of VitE (SME), or 0.1 mg organic Se (SMSe), or their combination (60 mg VitE and 0,1 mg organic Se/kg of concentrate (SMESe). In the plasma of the goats, the dietary treatments did not affect glutathione reductase, glutathione peroxidase, glutathione transferase, catalase, superoxide dismutase activities, malondialdehyde (MDA) content, or the total antioxidant capacity. A reduction and a trend for lower protein carbonyls content was found in goats fed SM (p = 0.03) and SME (p = 0.06) compared to SMESe. In the milk, the lactoperoxidase activity decreased with SMSe and SMESe. A numerical decrease in the total antioxidant capacity and an increase in the MDA content in the milk of the SMESe group compared with the other treated groups was found. In mid-lactation goats, SM improves the oxidative status of both the organism and the milk.

The objective of the present study was to investigate the effect of the natural flavonoid quercetin dietary supplementation on the alteration of egg metallome by applying the basic principles of elemental metabolomics. One hundred and ninety-two laying hens were allocated into 4 treatment groups: the control (C) group that was fed with a commercial basal diet and the other experimental groups that were offered the same diet further supplemented with quercetin at 200, 400 and 800 mg per kg of feed (Q2, Q4 and Q8 group, respectively) for 28 days. The diets contained the same vitamin and mineral premix, thus all birds received the same amount of elements since no differences on feed intake existed. The egg elemental profile consisted of As, Ca, Cd, Co, Cr, Cu, Fe, Mg, Mn, Mo, Ni, Pb, Sb, Se, Sr, V, Zn and was determined using inductively coupled plasma mass spectrometry (ICP-MS). Quercetin supplementation altered the elemental profile. Most notably, quercetin altered the element concentrations predominantly in egg shell and albumen. It increased the concentration of Sb while reduced that of Cr and Se in both egg shell and albumen. Moreover, it increased As, Cd in albumen and V in yolk, while compared to the control, reduced As, Cd, Cr, Cu and V and also raised Ca, Fe, Mg and Ni in egg shell. The presence of quercetin led to differentiation of the deposition of certain trace minerals in egg compartments compared to that of hens fed a basal diet, possibly indicating that tailor made eggs for specific nutritional and health requirements could be created in the future.