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Contamination of surface waters with pathogens as well as all diseases associated with such events are a significant concern worldwide. In recent decades, there has been a growing interest in developing analytical methods with good performance for the detection of this category of contaminants. The most important analytical methods applied for the determination of bacteria in waters are traditional ones (such as bacterial culturing methods, enzyme-linked immunoassay, polymerase chain reaction, and loop-mediated isothermal amplification) and advanced alternative methods (such as spectrometry, chromatography, capillary electrophoresis, surface-enhanced Raman scattering, and magnetic field-assisted and hyphenated techniques). In addition, optical and electrochemical sensors have gained much attention as essential alternatives for the conventional detection of bacteria. The large number of available methods have been materialized by many publications in this field aimed to ensure the control of water quality in water resources. This study represents a critical synthesis of the literature regarding the latest analytical methods covering comparative aspects of pathogen contamination of water resources. All these aspects are presented as representative examples, focusing on two important bacteria with essential implications on the health of the population, namely Pseudomonas aeruginosa and Escherichia coli.
Alexandra Canciu; Mihaela Tertis; Oana Hosu; Andreea Cernat; Cecilia Cristea; Florin Graur. Modern Analytical Techniques for Detection of Bacteria in Surface and Wastewaters. Sustainability 2021, 13, 7229 .
AMA StyleAlexandra Canciu, Mihaela Tertis, Oana Hosu, Andreea Cernat, Cecilia Cristea, Florin Graur. Modern Analytical Techniques for Detection of Bacteria in Surface and Wastewaters. Sustainability. 2021; 13 (13):7229.
Chicago/Turabian StyleAlexandra Canciu; Mihaela Tertis; Oana Hosu; Andreea Cernat; Cecilia Cristea; Florin Graur. 2021. "Modern Analytical Techniques for Detection of Bacteria in Surface and Wastewaters." Sustainability 13, no. 13: 7229.
Food safety and quality control pose serious issues to food industry and public health domains, in general, with direct effects on consumers. Any physical, chemical, or biological unexpected or unidentified food constituent may exhibit harmful effects on people and animals from mild to severe reactions. According to the World Health Organization (WHO), unsafe foodstuffs are especially dangerous for infants, young children, elderly, and chronic patients. It is imperative to continuously develop new technologies to detect foodborne pathogens and contaminants in order to aid the strengthening of healthcare and economic systems. In recent years, peptide-based sensors gained much attention in the field of food research as an alternative to immuno-, apta-, or DNA-based sensors. This review presents an overview of the electrochemical biosensors using peptides as molecular bio-recognition elements published mainly in the last decade, highlighting their possible application for rapid, non-destructive, and in situ analysis of food samples. Comparison with peptide-based optical and piezoelectrical sensors in terms of analytical performance is presented. Methods of foodstuffs pretreatment are also discussed.
Mihaela Tertis; Oana Hosu; Bogdan Feier; Andreea Cernat; Anca Florea; Cecilia Cristea. Electrochemical Peptide-Based Sensors for Foodborne Pathogens Detection. Molecules 2021, 26, 3200 .
AMA StyleMihaela Tertis, Oana Hosu, Bogdan Feier, Andreea Cernat, Anca Florea, Cecilia Cristea. Electrochemical Peptide-Based Sensors for Foodborne Pathogens Detection. Molecules. 2021; 26 (11):3200.
Chicago/Turabian StyleMihaela Tertis; Oana Hosu; Bogdan Feier; Andreea Cernat; Anca Florea; Cecilia Cristea. 2021. "Electrochemical Peptide-Based Sensors for Foodborne Pathogens Detection." Molecules 26, no. 11: 3200.
Despite the fact that the electrochemical biosensors based on glucose oxidase represent the golden standard for the management of diabetes, the elaboration of nonenzymatic sensors became extensively studied as an out-of-the-box concept that aims to simplify the existing approach. An important point of view is represented by the low price of the sensing device that has positive effects for both end-users and healthcare systems. The enzyme-free sensors based on low-cost materials such as transition metals have similar analytical properties to the commercial ones while eliminating the issues associated with the presence of the enzyme, such as the stability issues and limited shelf-life. The development of nanoporous nanomaterials for biomedical applications and electrocatalysis was referred to as an alternative to the conventional methods due to their enlarged area, electrical properties, ease of functionalization and not least to their low cost. Herein, we report the development of an electrochemical nonenzymatic sensor for glucose based on 3D copper nanostructures with Ni foams as promotor of the enhanced nanoporous morphology. The sensors were successfully tested in the presence of the designated target, even in the presence of common interference agents found in biological samples.
Gheorghe Melinte; Andreea Cernat; Aurora Petica; Oana Lazar; Marius Enachescu; Liana Anicai; Cecilia Cristea. Electrochemical Non-Enzymatic Detection of Glucose Based on 3D Electroformed Copper on Ni Foam Nanostructures. Materials 2020, 13, 1 .
AMA StyleGheorghe Melinte, Andreea Cernat, Aurora Petica, Oana Lazar, Marius Enachescu, Liana Anicai, Cecilia Cristea. Electrochemical Non-Enzymatic Detection of Glucose Based on 3D Electroformed Copper on Ni Foam Nanostructures. Materials. 2020; 13 (12):1.
Chicago/Turabian StyleGheorghe Melinte; Andreea Cernat; Aurora Petica; Oana Lazar; Marius Enachescu; Liana Anicai; Cecilia Cristea. 2020. "Electrochemical Non-Enzymatic Detection of Glucose Based on 3D Electroformed Copper on Ni Foam Nanostructures." Materials 13, no. 12: 1.
The detection of folic acid in biological samples or pharmaceutical products is of great importance due to its implications in the biological functions of the human body, along with the development and growth of the fetus. The deficiency of folic acid can be reversed by the intake of different pharmaceutical formulations or alimentary products fortified with this molecule. The elaboration of sensing platforms represents a continuous work in progress, a task in which the use of conductive polymers modified with different functionalities represents one of the outcoming strategies. The possibility of manipulating their morphology with the use of templates or surfactants represents another advantage. A sensing platform based on carboxylic functionalized polypyrrole was synthesized via the electrochemical approach in the presence of a polymeric surfactant on a graphite-based surface. The sensor was able to detect the folic acid from 2.5 μM to 200 μM with a calculated limited of detection of 0.8 μM. It was employed for the detection of the analyte from commercial human serum and pharmaceutical products with excellent recovery rates. The results were double checked using an optimized spectrophotometric procedure that confirmed furthermore the performances of the sensor related to real samples assessment.
Gheorghe Melinte; Andreea Cernat; Maria-Bianca Irimes; Szabolcs János Györfi; Mihaela Tertiș; Maria Suciu; Liana Anicăi; Robert Săndulescu; Cecilia Cristea. Selective Detection of Folic Acid Using 3D Polymeric Structures of 3-Carboxylic Polypyrrole. Sensors 2020, 20, 2315 .
AMA StyleGheorghe Melinte, Andreea Cernat, Maria-Bianca Irimes, Szabolcs János Györfi, Mihaela Tertiș, Maria Suciu, Liana Anicăi, Robert Săndulescu, Cecilia Cristea. Selective Detection of Folic Acid Using 3D Polymeric Structures of 3-Carboxylic Polypyrrole. Sensors. 2020; 20 (8):2315.
Chicago/Turabian StyleGheorghe Melinte; Andreea Cernat; Maria-Bianca Irimes; Szabolcs János Györfi; Mihaela Tertiș; Maria Suciu; Liana Anicăi; Robert Săndulescu; Cecilia Cristea. 2020. "Selective Detection of Folic Acid Using 3D Polymeric Structures of 3-Carboxylic Polypyrrole." Sensors 20, no. 8: 2315.
Pyoverdine is a fluorescent siderophore produced by Pseudomonas aeruginosa that can be considered as a detectable marker in nosocomial infections. The presence of pyoverdine in water can be directly linked to the presence of the P. aeruginosa, thus being a nontoxic and low-cost marker for the detection of biological contamination. A novel platform was developed and applied for the electrochemical selective and sensitive detection of pyoverdine, based on a graphene/graphite-modified screen-printed electrode (SPE) that was electrochemically reduced and decorated with gold nanoparticles (NPs). The optimized sensor presenting higher sensitivity towards pyoverdine was successfully applied for its detection in real samples (serum, saliva, and tap water), in the presence of various interfering species. The excellent analytical performances underline the premises for an early diagnosis kit of bacterial infections based on electrochemical sensors.
Islem Gandouzi; Mihaela Tertis; Andreea Cernat; Dalila Saidane-Mosbahi; Aranka Ilea; Cecilia Cristea. A Nanocomposite Based on Reduced Graphene and Gold Nanoparticles for Highly Sensitive Electrochemical Detection of Pseudomonas aeruginosa through Its Virulence Factors. Materials 2019, 12, 1180 .
AMA StyleIslem Gandouzi, Mihaela Tertis, Andreea Cernat, Dalila Saidane-Mosbahi, Aranka Ilea, Cecilia Cristea. A Nanocomposite Based on Reduced Graphene and Gold Nanoparticles for Highly Sensitive Electrochemical Detection of Pseudomonas aeruginosa through Its Virulence Factors. Materials. 2019; 12 (7):1180.
Chicago/Turabian StyleIslem Gandouzi; Mihaela Tertis; Andreea Cernat; Dalila Saidane-Mosbahi; Aranka Ilea; Cecilia Cristea. 2019. "A Nanocomposite Based on Reduced Graphene and Gold Nanoparticles for Highly Sensitive Electrochemical Detection of Pseudomonas aeruginosa through Its Virulence Factors." Materials 12, no. 7: 1180.
Early screening of clinically-relevant pathogens in the environment is a highly desirable goal in clinical care, providing precious information that will improve patient care outcomes. In this work, a glove-based electrochemical sensor has been designed for point-of-use screening of virulence factors, towards Pseudomonas aeruginosa detection. The methodology used for the elaboration of the fabric platform relied on printing the conductive inks on the index and middle fingers of the glove, with the goal of screening pyocyanin and pyoverdine targets. The analytical signatures of the analytes were recorded in about 4 minutes, via the rapid and selective square-wave voltammetry technique. Finger-based sensors display a good performance and discrimination against potential interferences, along with good reproducibility. The sensors featured linearity over the 0.01- 0.1 µM range for pyocyanin and 5-50 μM range for pyoverdine, with a sensitivity of 2.51 µA/µM for pyocyanin and 1.09 nA/μM for pyoverdine (R2=0.990 and 0.995, respectively), and detection limits of 3.33 nM for pyocyanin and 1.66 µM for pyoverdine. Moreover, in order to mimic real-life scenarios, the sensors were tested in the binary mixtures of analytes, with successful outcomes. In order to gain information from the surrounding environment, the active electronic area of printed fingers was coated with a conductive hydrogel matrix and relevant target surfaces were ‘swiped for notification’ of the contaminants. The simple fabrication, low-cost and the reusability features of the proposed glove are likely to underpin the progressive drive of wearable sensors towards decentralized environmental and healthcare applications.
Bianca Ciui; Mihaela Tertiş; Andreea Cernat; Robert Săndulescu; Joseph Wang; Cecilia Cristea. Finger-Based Printed Sensors Integrated on a Glove for On-Site Screening Of Pseudomonas aeruginosa Virulence Factors. Analytical Chemistry 2018, 90, 7761 -7768.
AMA StyleBianca Ciui, Mihaela Tertiş, Andreea Cernat, Robert Săndulescu, Joseph Wang, Cecilia Cristea. Finger-Based Printed Sensors Integrated on a Glove for On-Site Screening Of Pseudomonas aeruginosa Virulence Factors. Analytical Chemistry. 2018; 90 (12):7761-7768.
Chicago/Turabian StyleBianca Ciui; Mihaela Tertiş; Andreea Cernat; Robert Săndulescu; Joseph Wang; Cecilia Cristea. 2018. "Finger-Based Printed Sensors Integrated on a Glove for On-Site Screening Of Pseudomonas aeruginosa Virulence Factors." Analytical Chemistry 90, no. 12: 7761-7768.
The design and development of an electrochemical sensor for the sensitive and selective determination of pyoverdine, a virulence factor secreted by Pseudomonas aeruginosa, bacteria involved in nosocomial infections is presented in this work. The presence of pyoverdine in water and body fluids samples can be directly linked to the presence of the Pseudomonas bacteria, thus being a nontoxic and low cost marker for the detection of water pollution as well as for the biological contamination of other media. The sensor was elaborated using layer-by-layer technique for the deposition of a graphene‑gold nanoparticles composite film on the graphite-based screen printed electrode, from aqueous suspension. Under optimal conditions, the electrochemical signal corresponding to the pyoverdine oxidation process was proportional to its concentration, showing a wide linear range from 1 to 100μmolL-1 and a detection limit of 0.33μmolL-1. This sensor discriminate with satisfactory recoveries the target analyte in different real matrices and also exhibited low response to other interfering species, proving that this technique is promising for medical and environmental applications. In addition, the proposed nanocomposite platform presented good reproducibility, high and long term stability, the sensitivity for pyoverdine remain unchanged after being stored at 4°C for four weeks.
Islem Gandouzi; Mihaela Tertis; Andreea Cernat; Amina Bakhrouf; Maria Coros; Stela Maria Pruneanu; Cecilia Cristea. Sensitive detection of pyoverdine with an electrochemical sensor based on electrochemically generated graphene functionalized with gold nanoparticles. Bioelectrochemistry 2018, 120, 94 -103.
AMA StyleIslem Gandouzi, Mihaela Tertis, Andreea Cernat, Amina Bakhrouf, Maria Coros, Stela Maria Pruneanu, Cecilia Cristea. Sensitive detection of pyoverdine with an electrochemical sensor based on electrochemically generated graphene functionalized with gold nanoparticles. Bioelectrochemistry. 2018; 120 ():94-103.
Chicago/Turabian StyleIslem Gandouzi; Mihaela Tertis; Andreea Cernat; Amina Bakhrouf; Maria Coros; Stela Maria Pruneanu; Cecilia Cristea. 2018. "Sensitive detection of pyoverdine with an electrochemical sensor based on electrochemically generated graphene functionalized with gold nanoparticles." Bioelectrochemistry 120, no. : 94-103.
Andreea Cernat; Mihaela Tertis; Claudia Nicoleta Păpară; Ede Bodoki; Robert Săndulescu. Nanostructured Platform Based on Graphene-polypyrrole Composite for Immunosensor Fabrication. Procedia Technology 2017, 27, 108 -109.
AMA StyleAndreea Cernat, Mihaela Tertis, Claudia Nicoleta Păpară, Ede Bodoki, Robert Săndulescu. Nanostructured Platform Based on Graphene-polypyrrole Composite for Immunosensor Fabrication. Procedia Technology. 2017; 27 ():108-109.
Chicago/Turabian StyleAndreea Cernat; Mihaela Tertis; Claudia Nicoleta Păpară; Ede Bodoki; Robert Săndulescu. 2017. "Nanostructured Platform Based on Graphene-polypyrrole Composite for Immunosensor Fabrication." Procedia Technology 27, no. : 108-109.
A new biomimetic electrochemical sensor was developed for the detection of dopamine based on glassy carbon electrode modified with electrochemically generated gold nanoparticles. The preparation of the polymer is simple and cost-effective, achieving the polymerization of thioaniline and generation of gold nanoparticles in a single step by cyclic voltammetry, in the presence of the target molecule, dopamine. After extraction, the imprinted polymer exhibits high sensitivity and selectivity for dopamine. Moreover, the developed imprinted polymer film allows the fast, direct detection of dopamine without the need of a redox mediator. The formation of a self-assembly monolayer of the monomer prior to electropolymerization ensures the adherence of the film onto the electrode surface conferring good stability to the sensor (over two weeks). Cyclic voltammetry, electrochemical impedance spectroscopy, atomic force microscopy, scanning electron microscopy and energy dispersive X-ray spectroscopy were used for the complete characterization of the developed sensor, while differential pulse voltammetry was used for its testing.
Mihaela Tertis; Anca Florea; Alina Adumitrachioaie; Andreea Cernat; Diana Bogdan; Lucian Barbu-Tudoran; Nicole Jaffrezic-Renault; Robert Sandulescu; Cecilia Cristea. Detection of Dopamine by a Biomimetic Electrochemical Sensor Based on Polythioaniline-Bridged Gold Nanoparticles. ChemPlusChem 2016, 82, 561 -569.
AMA StyleMihaela Tertis, Anca Florea, Alina Adumitrachioaie, Andreea Cernat, Diana Bogdan, Lucian Barbu-Tudoran, Nicole Jaffrezic-Renault, Robert Sandulescu, Cecilia Cristea. Detection of Dopamine by a Biomimetic Electrochemical Sensor Based on Polythioaniline-Bridged Gold Nanoparticles. ChemPlusChem. 2016; 82 (4):561-569.
Chicago/Turabian StyleMihaela Tertis; Anca Florea; Alina Adumitrachioaie; Andreea Cernat; Diana Bogdan; Lucian Barbu-Tudoran; Nicole Jaffrezic-Renault; Robert Sandulescu; Cecilia Cristea. 2016. "Detection of Dopamine by a Biomimetic Electrochemical Sensor Based on Polythioaniline-Bridged Gold Nanoparticles." ChemPlusChem 82, no. 4: 561-569.
This chapter contains sections titled:
Andreea Cernat; Mihaela Tertiş; Luminiţa Fritea; Cecilia Cristea. Graphene in Sensors Design. Advanced 2D Materials 2016, 387 -431.
AMA StyleAndreea Cernat, Mihaela Tertiş, Luminiţa Fritea, Cecilia Cristea. Graphene in Sensors Design. Advanced 2D Materials. 2016; ():387-431.
Chicago/Turabian StyleAndreea Cernat; Mihaela Tertiş; Luminiţa Fritea; Cecilia Cristea. 2016. "Graphene in Sensors Design." Advanced 2D Materials , no. : 387-431.
A simple label‐free immunosensor for the selective detection of acetaminophen was developed by modifying a graphite based screen‐printed electrode with graphene oxide after the functionalization with N‐hydroxysuccinimide in the presence of 1‐ethyl‐3‐(3‐dimethyl aminopropyl) carbodiimide hydrochloride. The template made by layer‐by‐layer technique provides activated carboxylic groups, which form amidic covalent bonds with the terminal amine groups from the antiacetaminophen antibody. The optimization of antibody immobilization and the blocking of the free active groups from graphene template with bovine serum albumin was performed by using electrochemical impedance spectroscopy, cyclic voltammetry and square wave voltammetry. The electrochemical quartz crystal microbalance technique was used to determine the quantity of different components deposited onto the electrode surface during the optimization steps. The above described immunosensor was applied with good results for the determination of acetaminophen in synthetic and real samples by using square wave voltammetry. A limit of detection of 0.17 µM (S/N=3) was obtained with no or minimal interference of dosage forms excipients and serum components.
Mihaela Tertiş; Oana Hosu; Luminiţa Fritea; Cosmin Farcau; Andreea Cernat; Robert Săndulescu; Cecilia Cristea. A Novel Label-Free Immunosensor Based on Activated Graphene Oxide for Acetaminophen Detection. Electroanalysis 2015, 27, 638 -647.
AMA StyleMihaela Tertiş, Oana Hosu, Luminiţa Fritea, Cosmin Farcau, Andreea Cernat, Robert Săndulescu, Cecilia Cristea. A Novel Label-Free Immunosensor Based on Activated Graphene Oxide for Acetaminophen Detection. Electroanalysis. 2015; 27 (3):638-647.
Chicago/Turabian StyleMihaela Tertiş; Oana Hosu; Luminiţa Fritea; Cosmin Farcau; Andreea Cernat; Robert Săndulescu; Cecilia Cristea. 2015. "A Novel Label-Free Immunosensor Based on Activated Graphene Oxide for Acetaminophen Detection." Electroanalysis 27, no. 3: 638-647.
We propose the fabrication of nanostructured glassy carbon (GC) electrodes modified with horseradish peroxidase (HRP) for the detection of paracetamol. This was accomplished by inducing the nanostructuration of GC via the adsorption of polystyrene nanospheres (900 nm diameter) followed by electropolymerization of N-(10-azidodecyl)pyrrole. The nanospheres were then removed and nanostructured polypyrrole-GC was submitted to click reaction in presence of ethynyl-biotin that was further coupled to HRP-avidin. The electrode was then used to sense the electrochemical reduction of the enzymatically generated electroactive oxidized species of acetaminophen (NAPQI) in the presence of hydrogen peroxide. The nanostructured electrode with HRP exhibits a fast response towards NAPQI reduction and improved performances in terms of sensitivity and limit of detection compared to non structured electrode.
Andreea Cernat; Sophie Griveau; Cyrille Richard; Fethi Bedioui; Robert Săndulescu. Horseradish Peroxidase Nanopatterned Electrodes by Click Chemistry: Application to the Electrochemical Detection of Paracetamol. Electroanalysis 2013, 25, 1369 -1372.
AMA StyleAndreea Cernat, Sophie Griveau, Cyrille Richard, Fethi Bedioui, Robert Săndulescu. Horseradish Peroxidase Nanopatterned Electrodes by Click Chemistry: Application to the Electrochemical Detection of Paracetamol. Electroanalysis. 2013; 25 (6):1369-1372.
Chicago/Turabian StyleAndreea Cernat; Sophie Griveau; Cyrille Richard; Fethi Bedioui; Robert Săndulescu. 2013. "Horseradish Peroxidase Nanopatterned Electrodes by Click Chemistry: Application to the Electrochemical Detection of Paracetamol." Electroanalysis 25, no. 6: 1369-1372.
Andreea Cernat; Sophie Griveau; Pascal Martin; Jean Christophe Lacroix; Cosmin Farcau; Robert Sandulescu; Fethi Bedioui. Electrografted nanostructured platforms for click chemistry. Electrochemistry Communications 2012, 23, 141 -144.
AMA StyleAndreea Cernat, Sophie Griveau, Pascal Martin, Jean Christophe Lacroix, Cosmin Farcau, Robert Sandulescu, Fethi Bedioui. Electrografted nanostructured platforms for click chemistry. Electrochemistry Communications. 2012; 23 ():141-144.
Chicago/Turabian StyleAndreea Cernat; Sophie Griveau; Pascal Martin; Jean Christophe Lacroix; Cosmin Farcau; Robert Sandulescu; Fethi Bedioui. 2012. "Electrografted nanostructured platforms for click chemistry." Electrochemistry Communications 23, no. : 141-144.