This page has only limited features, please log in for full access.

Unclaimed
O Lee
Department of Bioindustry and Bioresource Engineering, Plant Engineering Research Institute, Sejong University, Seoul 05006, Korea

Honors and Awards

The user has no records in this section


Career Timeline

The user has no records in this section.


Short Biography

The user biography is not available.
Following
Followers
Co Authors
The list of users this user is following is empty.
Following: 0 users

Feed

Journal article
Published: 26 July 2021 in Plants
Reads 0
Downloads 0

Pyramiding useful QTLs into an elite variety is a promising strategy to develop tolerant varieties against multiple abiotic stresses. However, some QTLs may not be functionally compatible when they are introgressed into the same variety. Here, we tested the functional compatibility of Pup1 and Sub1, major QTLs for tolerance to phosphorus (P)-deficiency and submergence conditions, respectively. Phenotypic analysis revealed that IR64-Pup1+Sub1 (IPS) plants harboring both Pup1 and Sub1 QTLs show significant tolerance to submerged conditions, similarly to IR64-Sub1, while IPS failed to tolerate P deficiency and mild drought conditions; only IR64-Pup1 showed P deficiency tolerance. In submerged conditions, Sub1A and OsPSTOL1, major genes for Sub1 and Pup1 QTLs, respectively, were expressed at the same levels as in IPS and IR64-Sub1 and in IPS and IR64-Pup1, respectively. On the other hand, in P-non-supplied condition, crown root number, root length, and OsPSTOL1 expression level were significantly lower in IPS compared to those of IR64-Pup1. However, there was no significant difference in P content between IPS and IR64-Pup1. These results imply that Pup1 does not compromise Sub1 function in submerged condition, while Sub1 suppresses Pup1 function in P-non-supplied condition, possibly by regulating the transcript level of Pup1. In conclusion, Pup1 and Sub1 are regarded as functionally compatible under submergence condition but not under P-non-supplied condition. Further study is needed to elucidate the functional incompatibility of Pup1 and Sub1 QTLs in IPS under P-non-supplied condition.

ACS Style

Na-Hyun Shin; O Lee; Jae-Hyuk Han; Kihwan Song; Hee-Jong Koh; Soo-Cheul Yoo; Joong Chin. The Effect of Water Level in Rice Cropping System on Phosphorus Uptake Activity of Pup1 in a Pup1+Sub1 Breeding Line. Plants 2021, 10, 1523 .

AMA Style

Na-Hyun Shin, O Lee, Jae-Hyuk Han, Kihwan Song, Hee-Jong Koh, Soo-Cheul Yoo, Joong Chin. The Effect of Water Level in Rice Cropping System on Phosphorus Uptake Activity of Pup1 in a Pup1+Sub1 Breeding Line. Plants. 2021; 10 (8):1523.

Chicago/Turabian Style

Na-Hyun Shin; O Lee; Jae-Hyuk Han; Kihwan Song; Hee-Jong Koh; Soo-Cheul Yoo; Joong Chin. 2021. "The Effect of Water Level in Rice Cropping System on Phosphorus Uptake Activity of Pup1 in a Pup1+Sub1 Breeding Line." Plants 10, no. 8: 1523.

Journal article
Published: 06 July 2021 in Plants
Reads 0
Downloads 0

Red radish (Raphanus sativus L.) cultivars are a rich source of health-promoting anthocyanins and are considered a potential source of natural colorants used in the cosmetic industry. However, the development of red radish cultivars via conventional breeding is very difficult, given the unusual inheritance of the anthocyanin accumulation trait in radishes. Therefore, molecular markers linked with radish color are needed to facilitate radish breeding. Here, we characterized the RsTT8 gene isolated from four radish genotypes with different skin and flesh colors. Sequence analysis of RsTT8 revealed a large number of polymorphisms, including insertion/deletions (InDels), single nucleotide polymorphisms (SNPs), and simple sequence repeats (SSRs), between the red-fleshed and white-fleshed radish cultivars. To develop molecular markers on the basis of these polymorphisms for discriminating between radish genotypes with different colored flesh tissues, we designed four primer sets specific to the RsTT8 promoter, InDel, SSR, and WD40/acidic domain (WD/AD), and tested these primers on a diverse collection of radish lines. Except for the SSR-specific primer set, all primer sets successfully discriminated between red-fleshed and white-fleshed radish lines. Thus, we developed three molecular markers that can be efficiently used for breeding red-fleshed radish cultivars.

ACS Style

Soyun Kim; Keunho Yun; Han Park; Ju Ahn; Ju Yang; Hayoung Song; O Lee; Yoonkang Hur; Man-Ho Oh. Development of Molecular Markers for Predicting Radish (Raphanus sativus) Flesh Color Based on Polymorphisms in the RsTT8 Gene. Plants 2021, 10, 1386 .

AMA Style

Soyun Kim, Keunho Yun, Han Park, Ju Ahn, Ju Yang, Hayoung Song, O Lee, Yoonkang Hur, Man-Ho Oh. Development of Molecular Markers for Predicting Radish (Raphanus sativus) Flesh Color Based on Polymorphisms in the RsTT8 Gene. Plants. 2021; 10 (7):1386.

Chicago/Turabian Style

Soyun Kim; Keunho Yun; Han Park; Ju Ahn; Ju Yang; Hayoung Song; O Lee; Yoonkang Hur; Man-Ho Oh. 2021. "Development of Molecular Markers for Predicting Radish (Raphanus sativus) Flesh Color Based on Polymorphisms in the RsTT8 Gene." Plants 10, no. 7: 1386.

Journal article
Published: 21 June 2021 in Genes
Reads 0
Downloads 0

Lettuce plants tend to undergo floral initiation by elongation of flower stalks (bolting) under high-temperature and long-day conditions, which is a serious problem for summer lettuce production. Our objective was to generate a high-density genetic map using SNPs obtained from genotyping-by-sequencing (GBS) analysis of F5 recombinant inbred lines (RILs) and to map QTLs involved in stem growth and flowering time in lettuce. A set of 127 intra-specific RIL mapping populations derived from a cross between two varieties, green and red leaf lettuce, were used to identify QTLs related to the number of days from sowing to bolting (DTB), to flowering of the first flower (DTF), to seed-setting of the first flower (DTS), and the total number of leaves (LN), plant height (PH), and total number of branches of main inflorescence (BN) for two consecutive years. Of the 15 QTLs detected, one that controls DTB, DTF, DTS, LN, and PH detected on LG 7, and another QTL that controls DTF, DTS, and PH detected on LG 1. Analysis of the genomic sequence corresponding to the QTL detected on LG 7 led to the identification of 22 putative candidate genes. A consistent QTL related to bolting and flowering time, and corresponding candidate genes has been reported. This study will be valuable in revealing the genetic basis of stem growth and flowering time in lettuce.

ACS Style

O Lee; Keita Fukushima; Han Park; Saneyuki Kawabata. QTL Analysis of Stem Elongation and Flowering Time in Lettuce Using Genotyping-by-Sequencing. Genes 2021, 12, 947 .

AMA Style

O Lee, Keita Fukushima, Han Park, Saneyuki Kawabata. QTL Analysis of Stem Elongation and Flowering Time in Lettuce Using Genotyping-by-Sequencing. Genes. 2021; 12 (6):947.

Chicago/Turabian Style

O Lee; Keita Fukushima; Han Park; Saneyuki Kawabata. 2021. "QTL Analysis of Stem Elongation and Flowering Time in Lettuce Using Genotyping-by-Sequencing." Genes 12, no. 6: 947.

Journal article
Published: 03 June 2021 in Genes
Reads 0
Downloads 0

Fusarium wilt (FW) is a fungal disease that causes severe yield losses in radish production. The most effective method to control the FW is the development and use of resistant varieties in cultivation. The identification of marker loci linked to FW resistance are expected to facilitate the breeding of disease-resistant radishes. In the present study, we applied an integrated framework of genome-wide association studies (GWAS) using genotyping-by-sequencing (GBS) to identify FW resistance loci among a panel of 225 radish accessions, including 58 elite breeding lines. Phenotyping was conducted by manual inoculation of seedlings with the FW pathogen, and scoring for the disease index was conducted three weeks after inoculation during two constitutive years. The GWAS analysis identified 44 single nucleotide polymorphisms (SNPs) and twenty putative candidate genes that were significantly associated with FW resistance. In addition, a total of four QTLs were identified from F2 population derived from a FW resistant line and a susceptible line, one of which was co-located with the SNPs on chromosome 7, detected in GWAS study. These markers will be valuable for molecular breeding programs and marker-assisted selection to develop FW resistant varieties of R. sativus.

ACS Style

O Lee; Hyunjin Koo; Jae Yu; Han Park. Genotyping-by-Sequencing-Based Genome-Wide Association Studies of Fusarium Wilt Resistance in Radishes (Raphanus sativus L.). Genes 2021, 12, 858 .

AMA Style

O Lee, Hyunjin Koo, Jae Yu, Han Park. Genotyping-by-Sequencing-Based Genome-Wide Association Studies of Fusarium Wilt Resistance in Radishes (Raphanus sativus L.). Genes. 2021; 12 (6):858.

Chicago/Turabian Style

O Lee; Hyunjin Koo; Jae Yu; Han Park. 2021. "Genotyping-by-Sequencing-Based Genome-Wide Association Studies of Fusarium Wilt Resistance in Radishes (Raphanus sativus L.)." Genes 12, no. 6: 858.

Journal article
Published: 12 November 2020 in Plants
Reads 0
Downloads 0

Mertensia maritima (L.) Gray is threatened with extinction owing to climate change, poor seed germination, and ocean warming. In vitro explant-culture is used for ex situ preservation and plantlet massive production. In vitro cell and organ cultures serve as an alternative plant material source to investigate the biological activities and phytochemical profiles of rare plants. We aimed to develop an efficient callus and shoot production protocol and investigate bioactive metabolites, antioxidants, and enzyme inhibitory potential of M. maritima calli, shoots, and in vivo seedlings. The effects of combinations of different plant growth regulators, 6-BA (N6-benzyladenine), 6-KN (Kinetin), TDZ (Thidiazuron), and NAA (1-Naphthylacetic acid), in MS (Murashige and Skoog) nutrient medium were studied. The highest callus proliferation was obtained after 5-week cultivation over a 16-h photoperiod on growth medium MS enriched with 4 µM each of 6-BA and NAA. The medium with 2 µM 6-BA and 4 µM 6-KN had the best shoot induction rate (91.1%) with a mean of 13.4 shoots. The combination of two cytokinins (6-BA and 6-KN) was found to be effective in M. maritima shoot regeneration. The rooting frequency was 100% in ½ MS with Indole-3-butyric acid (IBA 2 µM). The number of detected compounds and chemical composition in the M. maritima shoots and seedlings extracts were similar. The total amount of phenolics in the shoots was 216.4% and 369.5% higher than in seedlings and calli, respectively. The total amount of flavonoids in the shoots was 241.1% and 429.3% higher than in seedlings and calli, respectively. The best antioxidant activity was obtained in the shoots, followed by seedlings and calli. However, the order was seedlings > calli > shoots regarding metal chelating ability. The strongest acetylcholinesterase inhibition properties were obtained in the calli, followed by seedlings and shoots. However, the tested samples can be ranked as seedlings > shoots > calli in butylcholinestrase inhibition assay. This study is the first report on the enzyme inhibitory effects of M. maritima extracts, providing valuable contributions to the scientific community.

ACS Style

Kihwan Song; Iyyakkannu Sivanesan; Gunes Ak; Gokhan Zengin; Zoltán Cziáky; József Jekő; Kannan Rr Rengasamy; O New Lee; Doo Hwan Kim. Screening of Bioactive Metabolites and Biological Activities of Calli, Shoots, and Seedlings of Mertensia maritima (L.) Gray. Plants 2020, 9, 1551 .

AMA Style

Kihwan Song, Iyyakkannu Sivanesan, Gunes Ak, Gokhan Zengin, Zoltán Cziáky, József Jekő, Kannan Rr Rengasamy, O New Lee, Doo Hwan Kim. Screening of Bioactive Metabolites and Biological Activities of Calli, Shoots, and Seedlings of Mertensia maritima (L.) Gray. Plants. 2020; 9 (11):1551.

Chicago/Turabian Style

Kihwan Song; Iyyakkannu Sivanesan; Gunes Ak; Gokhan Zengin; Zoltán Cziáky; József Jekő; Kannan Rr Rengasamy; O New Lee; Doo Hwan Kim. 2020. "Screening of Bioactive Metabolites and Biological Activities of Calli, Shoots, and Seedlings of Mertensia maritima (L.) Gray." Plants 9, no. 11: 1551.

Journal article
Published: 26 October 2020 in Molecules
Reads 0
Downloads 0

This study aimed to investigate the impact of plant growth regulators, sucrose concentration, and the number of subcultures on axillary shoot multiplication, in vitro flowering, and somaclonal variation and to assess the phytochemical composition, antioxidant capacity, and enzyme inhibitory potential of in vitro-established callus, somaclonal variant, and normal green shoots of Catharanthus roseus. The highest shoot induction rate (95.8%) and highest number of shoots (23.6), with a mean length of 4.5 cm, were attained when the C. roseus nodal explants (0.6–1 cm in length) were cultivated in Murashige and Skoog (MS) medium with 2 µM thidiazuron, 1 µM 2-(1-naphthyl) acetic acid (NAA), and 4% sucrose. The in vitro flowering of C. roseus was affected by sucrose, and the number of subcultures had a significant effect on shoot multiplication and somaclonal variation. The highest levels of phenolics and flavonoids were found in normal green shoots, followed by those in somaclonal variant shoots and callus. The phytochemicals in C. roseus extracts were qualified using liquid chromatography–tandem mass spectrometry. A total of 39, 55, and 59 compounds were identified in the callus, somaclonal variant shoot, and normal green shoot tissues, respectively. The normal green shoot extracts exhibited the best free radical scavenging ability and reducing power activity. The strongest acetylcholinesterase inhibitory effects were found in the callus, with an IC50 of 0.65 mg/mL.

ACS Style

O. New Lee; Gunes Ak; Gokhan Zengin; Zoltán Cziáky; József Jekő; Kannan R.R. Rengasamy; Han Yong Park; Doo Hwan Kim; Iyyakkannu Sivanesan. Phytochemical Composition, Antioxidant Capacity, and Enzyme Inhibitory Activity in Callus, Somaclonal Variant, and Normal Green Shoot Tissues of Catharanthus roseus (L) G. Don. Molecules 2020, 25, 4945 .

AMA Style

O. New Lee, Gunes Ak, Gokhan Zengin, Zoltán Cziáky, József Jekő, Kannan R.R. Rengasamy, Han Yong Park, Doo Hwan Kim, Iyyakkannu Sivanesan. Phytochemical Composition, Antioxidant Capacity, and Enzyme Inhibitory Activity in Callus, Somaclonal Variant, and Normal Green Shoot Tissues of Catharanthus roseus (L) G. Don. Molecules. 2020; 25 (21):4945.

Chicago/Turabian Style

O. New Lee; Gunes Ak; Gokhan Zengin; Zoltán Cziáky; József Jekő; Kannan R.R. Rengasamy; Han Yong Park; Doo Hwan Kim; Iyyakkannu Sivanesan. 2020. "Phytochemical Composition, Antioxidant Capacity, and Enzyme Inhibitory Activity in Callus, Somaclonal Variant, and Normal Green Shoot Tissues of Catharanthus roseus (L) G. Don." Molecules 25, no. 21: 4945.

Journal article
Published: 03 September 2020 in Remote Sensing
Reads 0
Downloads 0

The radish is a delicious, healthy vegetable and an important ingredient to many side dishes and main recipes. However, climate change, pollinator decline, and especially Fusarium wilt cause a significant reduction in the cultivation area and the quality of the radish yield. Previous studies on plant disease identification have relied heavily on extracting features manually from images, which is time-consuming and inefficient. In addition to Red-Green-Blue (RGB) images, the development of near-infrared (NIR) sensors has enabled a more effective way to monitor the diseases and evaluate plant health based on multispectral imagery. Thus, this study compares two distinct approaches in detecting radish wilt using RGB images and NIR images taken by unmanned aerial vehicles (UAV). The main research contributions include (1) a high-resolution RGB and NIR radish field dataset captured by drone from low to high altitudes, which can serve several research purposes; (2) implementation of a superpixel segmentation method to segment captured radish field images into separated segments; (3) a customized deep learning-based radish identification framework for the extracted segmented images, which achieved remarkable performance in terms of accuracy and robustness with the highest accuracy of 96%; (4) the proposal for a disease severity analysis that can detect different stages of the wilt disease; (5) showing that the approach based on NIR images is more straightforward and effective in detecting wilt disease than the learning approach based on the RGB dataset.

ACS Style

L. Minh Dang; Hanxiang Wang; Yanfen Li; Kyungbok Min; Jin Tae Kwak; O. New Lee; Hanyong Park; Hyeonjoon Moon. Fusarium Wilt of Radish Detection using RGB and Near Infrared Images from Unmanned Aerial Vehicles. Remote Sensing 2020, 12, 2863 .

AMA Style

L. Minh Dang, Hanxiang Wang, Yanfen Li, Kyungbok Min, Jin Tae Kwak, O. New Lee, Hanyong Park, Hyeonjoon Moon. Fusarium Wilt of Radish Detection using RGB and Near Infrared Images from Unmanned Aerial Vehicles. Remote Sensing. 2020; 12 (17):2863.

Chicago/Turabian Style

L. Minh Dang; Hanxiang Wang; Yanfen Li; Kyungbok Min; Jin Tae Kwak; O. New Lee; Hanyong Park; Hyeonjoon Moon. 2020. "Fusarium Wilt of Radish Detection using RGB and Near Infrared Images from Unmanned Aerial Vehicles." Remote Sensing 12, no. 17: 2863.