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The objectives of this research were, first, to determine the concentrations of certain heavy metals in the edible tissue of wild brown trout (Salmo trutta trutta) from two different rivers located in the Abruzzi region (Italy), and then, to investigate the levels of variation in vitellogenin (VTG) associated with the presence of metalloestrogens. VTG is an effective indicator for endocrine disturbance, and an increase in the vitellogenin levels in male fish is widely employed as a biomarker of estrogenic contamination in the aquatic environment. The muscles of the trout were analyzed for As, Cd, Co, Cr, Ni, Pb, Al, and Zn using an inductively coupled plasma-mass spectrometer (ICP-MS), and Hg was measured using a direct mercury analyzer (DMA-80). The calculated values of the condition factor confirmed a healthy status for this species, indicating that the aquatic habit in both rivers is suitable for brown trout life. No significant difference in the concentrations of each metal were reported between the trout from the two rivers, and no significant difference for VTG levels were found between male and female fish. It is interesting to note the high concentrations of Al/Zn, while the Pb, Cd, and Hg concentrations in all of the samples were lower than those established by the European Commission.
Carmine Merola; Angelo Bisegna; Giovanni Angelozzi; Annamaria Conte; Maria Abete; Caterina Stella; Sabina Pederiva; Caterina Faggio; Nicoletta Riganelli; Monia Perugini. Study of Heavy Metals Pollution and Vitellogenin Levels in Brown Trout (Salmo trutta trutta) Wild Fish Populations. Applied Sciences 2021, 11, 4965 .
AMA StyleCarmine Merola, Angelo Bisegna, Giovanni Angelozzi, Annamaria Conte, Maria Abete, Caterina Stella, Sabina Pederiva, Caterina Faggio, Nicoletta Riganelli, Monia Perugini. Study of Heavy Metals Pollution and Vitellogenin Levels in Brown Trout (Salmo trutta trutta) Wild Fish Populations. Applied Sciences. 2021; 11 (11):4965.
Chicago/Turabian StyleCarmine Merola; Angelo Bisegna; Giovanni Angelozzi; Annamaria Conte; Maria Abete; Caterina Stella; Sabina Pederiva; Caterina Faggio; Nicoletta Riganelli; Monia Perugini. 2021. "Study of Heavy Metals Pollution and Vitellogenin Levels in Brown Trout (Salmo trutta trutta) Wild Fish Populations." Applied Sciences 11, no. 11: 4965.
In 2013, the European Union (EU) lifted the feed ban restriction, authorizing the use of non-ruminant (NR) processed animal proteins (PAPs) as ingredient in aquafeed. A further relaxation is soon expected, and NR PAPs will be allowed in next future in poultry and pig feed, avoiding cannibalism. Other potential hazards linked to PAPs as raw material should be evaluated. Antibiotics administered along the lifecycle of animals may leave residue in tissues and bones and still be present in PAPs. This monitoring study aimed to determine tetracyclines (TCLs), known to cumulate in bones, in PAPs and their possible residual antibiotic activity (RAC). A sensitive Liquid Chromatography coupled to Tandem Mass Spectrometry (LC-MS/MS) method for the quantification of TCLs in PAPs was developed and applied to 55 PAPs from EU manufactures. Most PAP samples (n = 40) contained TCLs (concentrations 25.59 ÷ 456.84 µg kg−1). Among samples containing more than 25 µg kg−1 for at least three TCLs, three PAPs were chosen for RAC test before and after TCLs extraction procedure applying an in vitro acidic digestion: in two out of those three samples, RAC was observed after in vitro digestion. TCLs were determined in the digested PAPs (concentrations 26.07 ÷ 64.55 µg kg−1). The detection of TCLs in PAPs should promptly target the risk assessments of this unconsidered way of exposure to antibiotic residues.
Sara Morello; Sabina Pederiva; Rosa Avolio; Giuseppina Amato; Simona Zoppi; Alessia Di Blasio; Maria Abete; Cristina Casalone; Rosanna Desiato; Giuseppe Ru; Daniela Marchis. Tetracyclines in Processed Animal Proteins: A Monitoring Study on Their Occurrence and Antimicrobial Activity. Foods 2021, 10, 696 .
AMA StyleSara Morello, Sabina Pederiva, Rosa Avolio, Giuseppina Amato, Simona Zoppi, Alessia Di Blasio, Maria Abete, Cristina Casalone, Rosanna Desiato, Giuseppe Ru, Daniela Marchis. Tetracyclines in Processed Animal Proteins: A Monitoring Study on Their Occurrence and Antimicrobial Activity. Foods. 2021; 10 (4):696.
Chicago/Turabian StyleSara Morello; Sabina Pederiva; Rosa Avolio; Giuseppina Amato; Simona Zoppi; Alessia Di Blasio; Maria Abete; Cristina Casalone; Rosanna Desiato; Giuseppe Ru; Daniela Marchis. 2021. "Tetracyclines in Processed Animal Proteins: A Monitoring Study on Their Occurrence and Antimicrobial Activity." Foods 10, no. 4: 696.
A portable measurement and speciation procedure for inorganic mercury (HgIN) and methylmercury (CH3Hg) was developed. A portable sample pretreatment was optimized to determine total mercury content. A new home-made sorbent (CYXAD, CHYPOS 101 modified Amberlite XAD), was prepared to separate HgIN and CH3Hg. Mercury species were determined using square wave anodic stripping voltammetry (SW-ASV) with a solid gold electrode (SGE) and using a portable potentiostat. A certified reference material, five freeze-dried samples and three fresh samples were analysed with conventional voltammetric analyzer, after dissolution of the samples in microwave oven, and with a portable potentiostat after the mild eating procedure. The results obtained by SW-ASV were compared with those obtained using Direct Mercury Analyser (DMA). The quantification with the portable method is comparable to that obtained with the DMA. Retention tests showed the selectivity of CYXAD for HgIN, its stability and the possibility to re-use the same aliquot of resin.
Agnese Giacomino; Andrea Ruo Redda; Rocco Caligiuri; Paolo Inaudi; Stefania Squadrone; Maria Cesarina Abete; Ornella Abollino; Sara Morandi; Eleonora Conca; Mery Malandrino. Development of an easy portable procedure for on-site determination of mercury and methylmercury. Food Chemistry 2020, 342, 128347 .
AMA StyleAgnese Giacomino, Andrea Ruo Redda, Rocco Caligiuri, Paolo Inaudi, Stefania Squadrone, Maria Cesarina Abete, Ornella Abollino, Sara Morandi, Eleonora Conca, Mery Malandrino. Development of an easy portable procedure for on-site determination of mercury and methylmercury. Food Chemistry. 2020; 342 ():128347.
Chicago/Turabian StyleAgnese Giacomino; Andrea Ruo Redda; Rocco Caligiuri; Paolo Inaudi; Stefania Squadrone; Maria Cesarina Abete; Ornella Abollino; Sara Morandi; Eleonora Conca; Mery Malandrino. 2020. "Development of an easy portable procedure for on-site determination of mercury and methylmercury." Food Chemistry 342, no. : 128347.
This study aimed to assess the biological and sanitary conditions of alien fish in a high-mountain lake (Balma Lake) located in the Cottian Alps. A single fish sampling session (August 2018) using gillnets collected 90 specimens of brook trout (Salvelinus fontinalis). Sex and age were determined (59 females and 31 males, age class 0+ to 4+). Regression analysis showed no difference in total weight and total length between males and females (ANCOVA: F = 0.453; p = 0.954). The mean condition factor (Kmean) decreased with increasing age for males and females. Terrestrial insects were the main prey found in the fish stomachs. The parasitological exam was negative, and the bacteriological exam was positive for Carnobacterium maltaromaticum and C. divergens in 33% of specimens. The total mercury, cadmium, and lead concentration in muscle tissue was within the maximum limit established by the European Commission for human consumption. The brook trout population was found to be well structured; these findings may help local administrations in the implementation of eradication measures.
Paolo Pastorino; Marino Prearo; Marco Bertoli; Vasco Menconi; Giuseppe Esposito; Marzia Righetti; Davide Mugetti; Sabina Pederiva; Maria Cesarina Abete; Elisabetta Pizzul. Assessment of Biological and Sanitary Condition of Alien Fish from a High-Mountain Lake (Cottian Alps). Water 2020, 12, 559 .
AMA StylePaolo Pastorino, Marino Prearo, Marco Bertoli, Vasco Menconi, Giuseppe Esposito, Marzia Righetti, Davide Mugetti, Sabina Pederiva, Maria Cesarina Abete, Elisabetta Pizzul. Assessment of Biological and Sanitary Condition of Alien Fish from a High-Mountain Lake (Cottian Alps). Water. 2020; 12 (2):559.
Chicago/Turabian StylePaolo Pastorino; Marino Prearo; Marco Bertoli; Vasco Menconi; Giuseppe Esposito; Marzia Righetti; Davide Mugetti; Sabina Pederiva; Maria Cesarina Abete; Elisabetta Pizzul. 2020. "Assessment of Biological and Sanitary Condition of Alien Fish from a High-Mountain Lake (Cottian Alps)." Water 12, no. 2: 559.
Identification and quantification of Non Intentionally Added Substances (NIAS) at low concentrations and eventually their distribution on Low Density Polyethylene (LDPE) films is an important issue. As Raman spectroscopy techniques are innovative and sensitive analytical methods and do not require pretreatment and manipulation of the sample, these were used for the identification of NIAS in LDPE pellet and films, which are commonly used for food packaging applications. The aggregation state of NIAS in LDPE were observed by Raman imaging with micrometric resolution. Four main NIAS compounds were identified in granule and film surfaces by confocal Raman spectroscopy: calcite (CaCO3), calcium sulphate (CaSO4), polystyrene (PS) and titanium dioxide (TiO2) in anatase form. By a semi-quantitative evaluation of the inorganic NIAS, 9.93 mg/kg of CaCO3, 1.62 mg/kg of CaSO4 and 0.17 mg/kg of TiO2 were estimated. The experimental findings achieved by Raman analysis were confirmed by the results obtained by Inductively Coupled Plasma-Mass Spectrometry (ICP-MS). Moreover, the toxicity of the identified compounds was examined according to the Cramer rules.
Chiara Portesi; Davide Visentin; Francesca Durbiano; Maria Cesarina Abete; Marco Rizzi; Valter Maurino; Andrea Mario Rossi. Development of a rapid micro-Raman spectroscopy approach for detection of NIAS in LDPE pellets and extruded films for food packaging applications. Polymer Testing 2019, 80, 106098 .
AMA StyleChiara Portesi, Davide Visentin, Francesca Durbiano, Maria Cesarina Abete, Marco Rizzi, Valter Maurino, Andrea Mario Rossi. Development of a rapid micro-Raman spectroscopy approach for detection of NIAS in LDPE pellets and extruded films for food packaging applications. Polymer Testing. 2019; 80 ():106098.
Chicago/Turabian StyleChiara Portesi; Davide Visentin; Francesca Durbiano; Maria Cesarina Abete; Marco Rizzi; Valter Maurino; Andrea Mario Rossi. 2019. "Development of a rapid micro-Raman spectroscopy approach for detection of NIAS in LDPE pellets and extruded films for food packaging applications." Polymer Testing 80, no. : 106098.
A simple procedure for field fish sample pretreatment was developed. This treatment in combination with square wave anodic stripping voltammetry (SW-ASV) with solid gold electrodes (SGE) and gold nanoparticle-modified glassy carbon electrodes (AuNPs-GCE) was applied for the determination of total mercury content. A certified reference material (CRM, Tuna Fish BCR 463), ten freeze-dried samples of canned tuna and two fresh fish samples were analysed both with a bench-top voltammetric analyser after microwave digestion and with a portable potentiostat after mild eating using a small commercial food warmer. The results obtained by the two SW-ASV approaches and by a Direct Mercury Analyser (DMA), the official method for mercury determination, were in very good agreement. In particular, (i) the results obtained with in field procedure are consistent with those obtained with the conventional microwave digestion; (ii) the presence of gold nanoparticles on the active electrode surface permits an improvement of the analytical performance in comparison to the SGE: the Limit of Quantification (LOQ) for mercury in fish-matrix was 0.1 μg L−1 (Hg cell concentration), corresponding to 0.06 mg kg−1 wet fish, which is a performance comparable to that of DMA. The pretreatment proposed in this study is very easy and applicable to fresh fish; in combination with a portable potentiostat, it proved to be an interesting procedure for on-site mercury determination.
Andrea Ruo Redda; Ornella Abollino; Mery Malandrino; Stefania Squadrone; Maria Cesarina Abete; Silvia Berto; Rosanna Toniolo; Francesca Durbiano; Agnese Giacomino. A Portable Setup for the Voltammetric Determination of Total Mercury in Fish with Solid and Nanostructured Gold Electrodes. Molecules 2019, 24, 1910 .
AMA StyleAndrea Ruo Redda, Ornella Abollino, Mery Malandrino, Stefania Squadrone, Maria Cesarina Abete, Silvia Berto, Rosanna Toniolo, Francesca Durbiano, Agnese Giacomino. A Portable Setup for the Voltammetric Determination of Total Mercury in Fish with Solid and Nanostructured Gold Electrodes. Molecules. 2019; 24 (10):1910.
Chicago/Turabian StyleAndrea Ruo Redda; Ornella Abollino; Mery Malandrino; Stefania Squadrone; Maria Cesarina Abete; Silvia Berto; Rosanna Toniolo; Francesca Durbiano; Agnese Giacomino. 2019. "A Portable Setup for the Voltammetric Determination of Total Mercury in Fish with Solid and Nanostructured Gold Electrodes." Molecules 24, no. 10: 1910.
The implementation of the European Union strategy for polychlorodibenzo-dioxins and -furans (PCDD/Fs), and dioxin-like polychlorobiphenyls (DL-PCBs) is determining a general reduction of their presence in the environment and in the food chain. The most important route for human exposure to these substances is food consumption and, as a consequence, a progressive decrease of their dietary intake has been observed in the last decades. In this context, it seemed worth updating the PCDD/F and DL-PCB intake estimation for the Italian population. A total of 2659 samples of food of animal and vegetable origin analyzed for PCDD/Fs and DL-PCBs in the period 2013–2016 by accredited official laboratories and the national food consumption database were considered for the dietary intake assessment in different age groups of the Italian general population The median cumulative intake estimates expressed as pg WHO-TEQ/kg body weight per day and computed with a deterministic and a probabilistic approach were 1.40–1.52 for children, 0.82–0.85 for adolescents, and 0.64–0.61 for adults, respectively. Such results confirm the decreasing trend of PCDD/F and DL-PCB dietary intake even though the Tolerable Daily Intake (TDI) value of 2 WHO-TEQ/kg body weight per day is exceeded at the 95th percentile for all age groups, with children as sensitive group. Most contributing food categories to the intake resulted fish, food of vegetable origin, and cheese. A sensitivity analysis was also performed to calculate the target contamination levels able to keep the dietary exposure below the TDI. Computed target levels fall between P50 and P97 of the occurrence distribution of the main food groups, meaning that most of the Italian food production can be considered safe.
Gianfranco Diletti; Giampiero Scortichini; Maria Cesarina Abete; Giovanni Binato; Luca Candeloro; Roberta Ceci; Giannina Chessa; Annamaria Conte; Alessandra Di Sandro; Mauro Esposito; Giorgio Fedrizzi; Vincenzo Ferrantelli; Enrica Ferretti; Simonetta Menotta; Valeria Nardelli; Bruno Neri; Arianna Piersanti; Francesca Roberti; Alessandro Ubaldi; Gianfranco Brambilla. Intake estimates of dioxins and dioxin-like polychlorobiphenyls in the Italian general population from the 2013-2016 results of official monitoring plans in food. Science of The Total Environment 2018, 627, 11 -19.
AMA StyleGianfranco Diletti, Giampiero Scortichini, Maria Cesarina Abete, Giovanni Binato, Luca Candeloro, Roberta Ceci, Giannina Chessa, Annamaria Conte, Alessandra Di Sandro, Mauro Esposito, Giorgio Fedrizzi, Vincenzo Ferrantelli, Enrica Ferretti, Simonetta Menotta, Valeria Nardelli, Bruno Neri, Arianna Piersanti, Francesca Roberti, Alessandro Ubaldi, Gianfranco Brambilla. Intake estimates of dioxins and dioxin-like polychlorobiphenyls in the Italian general population from the 2013-2016 results of official monitoring plans in food. Science of The Total Environment. 2018; 627 ():11-19.
Chicago/Turabian StyleGianfranco Diletti; Giampiero Scortichini; Maria Cesarina Abete; Giovanni Binato; Luca Candeloro; Roberta Ceci; Giannina Chessa; Annamaria Conte; Alessandra Di Sandro; Mauro Esposito; Giorgio Fedrizzi; Vincenzo Ferrantelli; Enrica Ferretti; Simonetta Menotta; Valeria Nardelli; Bruno Neri; Arianna Piersanti; Francesca Roberti; Alessandro Ubaldi; Gianfranco Brambilla. 2018. "Intake estimates of dioxins and dioxin-like polychlorobiphenyls in the Italian general population from the 2013-2016 results of official monitoring plans in food." Science of The Total Environment 627, no. : 11-19.
Human HT-29 and HepG2 cell lines were employed to test the effects of increasing concentrations of two rare earth elements (REEs), namely cerium (Ce) and lanthanum (La), alone or in combination. Effects on cell proliferation were measured using MTT assay, luciferase-based assays and proliferating cell nuclear antigen expression, while cell mortality and type of cell death was determined by Annexin V-FTC test using flow cytometry. Modulation of 84 genes involved in oxidative stress pathways was also studied using RT-PCR based arrays. Major alterations in selected genes compared to basal expression levels of respective control groups were found in the cells exposed to 600μM Ce for 48h. In HepG2 cells, 51 out of 84 genes were significantly up- or down-regulated, while in HT-29 cells only 16 genes were significantly up- or down-regulated. Dosage of REEs seems to be the pivotal factor for switching the biological effects from down- to up-regulation of cell growth; thus, low concentrations promoted cell survival and proliferation, but when concentrations increased, REEs exerted anti-proliferative and cytostatic/cytotoxic effects. The molecular mechanisms underlying these effects are still not well-defined and further analysis of the mechanisms that result in inhibition or induction of cell proliferation are crucially important.
Alessandro Benedetto; Claudia Bocca; Paola Brizio; Stefania Cannito; Maria Cesarina Abete; Stefania Squadrone. Effects of the rare elements lanthanum and cerium on the growth of colorectal and hepatic cancer cell lines. Toxicology in Vitro 2018, 46, 9 -18.
AMA StyleAlessandro Benedetto, Claudia Bocca, Paola Brizio, Stefania Cannito, Maria Cesarina Abete, Stefania Squadrone. Effects of the rare elements lanthanum and cerium on the growth of colorectal and hepatic cancer cell lines. Toxicology in Vitro. 2018; 46 ():9-18.
Chicago/Turabian StyleAlessandro Benedetto; Claudia Bocca; Paola Brizio; Stefania Cannito; Maria Cesarina Abete; Stefania Squadrone. 2018. "Effects of the rare elements lanthanum and cerium on the growth of colorectal and hepatic cancer cell lines." Toxicology in Vitro 46, no. : 9-18.
Marco Battuello; Nicola Nurra; Paola Brizio; Rocco Mussat Sartor; Daniela Pessani; Caterina Stella; Maria Cesarina Abete; Stefania Squadrone. The isopod Eurydice spinigera and the chaetognath Flaccisagitta enflata: How habitat affects bioaccumulation of metals in predaceous marine invertebrates. Ecological Indicators 2018, 84, 152 -160.
AMA StyleMarco Battuello, Nicola Nurra, Paola Brizio, Rocco Mussat Sartor, Daniela Pessani, Caterina Stella, Maria Cesarina Abete, Stefania Squadrone. The isopod Eurydice spinigera and the chaetognath Flaccisagitta enflata: How habitat affects bioaccumulation of metals in predaceous marine invertebrates. Ecological Indicators. 2018; 84 ():152-160.
Chicago/Turabian StyleMarco Battuello; Nicola Nurra; Paola Brizio; Rocco Mussat Sartor; Daniela Pessani; Caterina Stella; Maria Cesarina Abete; Stefania Squadrone. 2018. "The isopod Eurydice spinigera and the chaetognath Flaccisagitta enflata: How habitat affects bioaccumulation of metals in predaceous marine invertebrates." Ecological Indicators 84, no. : 152-160.
Stefania Squadrone; Paola Brizio; Marco Battuello; Nicola Nurra; Rocco Mussat Sartor; Alessandro Benedetto; Daniela Pessani; Maria Cesarina Abete. A first report of rare earth elements in northwestern Mediterranean seaweeds. Marine Pollution Bulletin 2017, 122, 236 -242.
AMA StyleStefania Squadrone, Paola Brizio, Marco Battuello, Nicola Nurra, Rocco Mussat Sartor, Alessandro Benedetto, Daniela Pessani, Maria Cesarina Abete. A first report of rare earth elements in northwestern Mediterranean seaweeds. Marine Pollution Bulletin. 2017; 122 (1-2):236-242.
Chicago/Turabian StyleStefania Squadrone; Paola Brizio; Marco Battuello; Nicola Nurra; Rocco Mussat Sartor; Alessandro Benedetto; Daniela Pessani; Maria Cesarina Abete. 2017. "A first report of rare earth elements in northwestern Mediterranean seaweeds." Marine Pollution Bulletin 122, no. 1-2: 236-242.
The applicability to the determination of mercury in tuna of square wave anodic stripping voltammetry (SW-ASV) conducted at both solid gold electrode (SGE) and a gold nanoparticle-modified glassy carbon electrode (AuNPs-GCE) was demonstrated. Mercury content in two certified materials and in ten samples of canned tuna was measured. The performances of the electrodes were compared with one another as well as with two spectroscopic techniques, namely cold vapour atomic absorption spectroscopy (CV-AAS) and a direct mercury analyser (DMA). The results found pointed out that both SW-ASV approaches were suitable and easy-to-use method to monitor mercury concentration in tunas, since they allowed accurate quantification at concentration values lower than the maximum admissible level in this matrix ([Hg]=1mg/kg). In particular, mercury detection at the AuNPs-GCE showed a LOQ in fish-matrix of 0.1μg/l, corresponding to 0.06mg/kg, with performance comparable to that of DMA.
Agnese Giacomino; Andrea Ruo Redda; Stefania Squadrone; Marco Rizzi; Maria Cesarina Abete; Carmela La Gioia; Rosanna Toniolo; Ornella Abollino; Mery Malandrino. Anodic stripping voltammetry with gold electrodes as an alternative method for the routine determination of mercury in fish. Comparison with spectroscopic approaches. Food Chemistry 2017, 221, 737 -745.
AMA StyleAgnese Giacomino, Andrea Ruo Redda, Stefania Squadrone, Marco Rizzi, Maria Cesarina Abete, Carmela La Gioia, Rosanna Toniolo, Ornella Abollino, Mery Malandrino. Anodic stripping voltammetry with gold electrodes as an alternative method for the routine determination of mercury in fish. Comparison with spectroscopic approaches. Food Chemistry. 2017; 221 ():737-745.
Chicago/Turabian StyleAgnese Giacomino; Andrea Ruo Redda; Stefania Squadrone; Marco Rizzi; Maria Cesarina Abete; Carmela La Gioia; Rosanna Toniolo; Ornella Abollino; Mery Malandrino. 2017. "Anodic stripping voltammetry with gold electrodes as an alternative method for the routine determination of mercury in fish. Comparison with spectroscopic approaches." Food Chemistry 221, no. : 737-745.
The risk of Hg poisoning by eating seafood is considered real from the several international agencies that recommended, by fish consumption advisories, to pregnant women and young children to avoid or severely limit the consumption of the fish and shellfish with a high-range mercury levels. The analyses of two common species, European hake and Norway lobster, collected from an area of Central Adriatic Sea, reported high mercury levels in crustaceans. For Norway lobster total mercury exceeded, in six out of ten analysed pools, the recommended 0.5mg/kg wet weight European limit. Moreover the increased amount of Hg concentrations in Norway lobster cooked samples suggests the necessity to review current procedures of Hg control in food, considering also consumption habits of consumers. The Hg values found in all European hake samples are below the legal limits and, in this species, the boiling did not modify the concentrations in fish tissues.
Monia Perugini; Daniela Zezza; Serena Maria Rita Tulini; Maria Cesarina Abete; Gabriella Monaco; Annamaria Conte; Vincenzo Olivieri; Michele Amorena. Effect of cooking on total mercury content in Norway lobster and European hake and public health impact. Marine Pollution Bulletin 2016, 109, 521 -525.
AMA StyleMonia Perugini, Daniela Zezza, Serena Maria Rita Tulini, Maria Cesarina Abete, Gabriella Monaco, Annamaria Conte, Vincenzo Olivieri, Michele Amorena. Effect of cooking on total mercury content in Norway lobster and European hake and public health impact. Marine Pollution Bulletin. 2016; 109 (1):521-525.
Chicago/Turabian StyleMonia Perugini; Daniela Zezza; Serena Maria Rita Tulini; Maria Cesarina Abete; Gabriella Monaco; Annamaria Conte; Vincenzo Olivieri; Michele Amorena. 2016. "Effect of cooking on total mercury content in Norway lobster and European hake and public health impact." Marine Pollution Bulletin 109, no. 1: 521-525.
PCDDs, PCDFs, and PCBs are persistent organic pollutants (POPs) that accumulate in animal products and may pose serious health problems. Those able to bind the aryl hydrocarbon receptor (AhR), eliciting a plethora of toxic responses, are defined dioxin-like (DL) compounds, while the remainders are called non-DL (NDL). An EFSA opinion has highlighted the tendency of ovine liver to specifically accumulate DL-compounds to a greater extent than any other farmed ruminant species. To examine the possible role in such an accumulation of xenobiotic metabolizing enzymes (XME) involved in DL-compound biotransformation, liver samples were collected from ewes and cows reared in an area known for low dioxin contamination. A related paper reported that sheep livers had about 5-fold higher DL-compound concentrations than cattle livers, while the content of the six marker NDL-PCBs did not differ between species. Specimens from the same animals were subjected to gene expression analysis for AhR, AhR nuclear translocator (ARNT) and AhR-dependent oxidative and conjugative pathways; XME protein expression and activities were also investigated. Both AhR and ARNT mRNA levels were about 2-fold lower in ovine samples and the same occurred for CYP1A1 and CYP1A2, being approximately 3- and 9-fold less expressed in sheep compared to cattle, while CYP1B1 could be detectable in cattle only. The results of the immunoblotting and catalytic activity (most notably EROD) measurements of the CYP1A family enzymes were in line with the gene expression data. By contrast, phase II enzyme expression and activities in sheep were higher (UGT1A) or similar (GSTA1, NQO1) to those recorded in cattle. The overall low expression of CYP1 family enzymes in the sheep is in line with the observed liver accumulation of DL-compounds and is expected to affect the kinetics and the dynamics of other POPs such as many polycyclic aromatic hydrocarbons, as well as of toxins (e.g. aflatoxins) or drugs (e.g. benzimidazole anthelmintics) known to be metabolized by those enzymes.
F. Girolami; V. Spalenza; A. Benedetto; L. Manzini; P. Badino; M.C. Abete; C. Nebbia. Comparative liver accumulation of dioxin-like compounds in sheep and cattle: Possible role of AhR-mediated xenobiotic metabolizing enzymes. Science of The Total Environment 2016, 571, 1222 -1229.
AMA StyleF. Girolami, V. Spalenza, A. Benedetto, L. Manzini, P. Badino, M.C. Abete, C. Nebbia. Comparative liver accumulation of dioxin-like compounds in sheep and cattle: Possible role of AhR-mediated xenobiotic metabolizing enzymes. Science of The Total Environment. 2016; 571 ():1222-1229.
Chicago/Turabian StyleF. Girolami; V. Spalenza; A. Benedetto; L. Manzini; P. Badino; M.C. Abete; C. Nebbia. 2016. "Comparative liver accumulation of dioxin-like compounds in sheep and cattle: Possible role of AhR-mediated xenobiotic metabolizing enzymes." Science of The Total Environment 571, no. : 1222-1229.
Stefania Squadrone; Daniela Marchis; Andrea Loria; Giuseppina Amato; Gian Luca Ferro; Maria Cesarina Abete. Detection of banned antibacterial growth promoter in animal feed by enzyme-linked immunosorbent assay: Method validation according to the Commission Decision 2002/657/EC criteria. Food Control 2015, 47, 66 -70.
AMA StyleStefania Squadrone, Daniela Marchis, Andrea Loria, Giuseppina Amato, Gian Luca Ferro, Maria Cesarina Abete. Detection of banned antibacterial growth promoter in animal feed by enzyme-linked immunosorbent assay: Method validation according to the Commission Decision 2002/657/EC criteria. Food Control. 2015; 47 ():66-70.
Chicago/Turabian StyleStefania Squadrone; Daniela Marchis; Andrea Loria; Giuseppina Amato; Gian Luca Ferro; Maria Cesarina Abete. 2015. "Detection of banned antibacterial growth promoter in animal feed by enzyme-linked immunosorbent assay: Method validation according to the Commission Decision 2002/657/EC criteria." Food Control 47, no. : 66-70.
This study aims to examine the possible presence of lead and cadmium in the liver and kidneys of hooded crows (Corvus cornix). Liver and kidneys of hooded crow carcasses were collected in Province of Cuneo (Piedmont, Italy) in order to detect lead and cadmium content. Significant differences were found in lead and cadmium levels between areas of intensive cultivation versus areas where meadows are prevalent. Moreover, age greatly influenced the burden of heavy metals, while sex did not seem to affect the level of contamination. The source of contamination may be phosphate fertilizers used for intensive cultivation in the study area.
Mauro Giammarino; Piero Quatto; Stefania Squadrone; Maria Cesarina Abete. The Hooded Crow (Corvus cornix) as an Environmental Bioindicator Species of Heavy Metal Contamination. Bulletin of Environmental Contamination and Toxicology 2014, 93, 410 -416.
AMA StyleMauro Giammarino, Piero Quatto, Stefania Squadrone, Maria Cesarina Abete. The Hooded Crow (Corvus cornix) as an Environmental Bioindicator Species of Heavy Metal Contamination. Bulletin of Environmental Contamination and Toxicology. 2014; 93 (4):410-416.
Chicago/Turabian StyleMauro Giammarino; Piero Quatto; Stefania Squadrone; Maria Cesarina Abete. 2014. "The Hooded Crow (Corvus cornix) as an Environmental Bioindicator Species of Heavy Metal Contamination." Bulletin of Environmental Contamination and Toxicology 93, no. 4: 410-416.
A Seafood Frequency Questionnaire (SFQ) broken down in more than 42 items with 8-week coverage was interview-administered to 278 adults aged 19-82years (167 women, 98 in the reproductive age 19-45years, and 111 men), resident on the Italian Mediterranean shore and frequent buyer at local fish markets. Methylmercury (MeHg) intake on individual basis was estimated for a selected occurrence equal to the median value+Median Absolute Deviation (MAD) in each seafood species reported (conservative scenario). MeHg occurrence was derived from an extensive seafood database referred to years 2009-2011. Accounting for an average body weight of 62.2kg, 24.6% of women resulted overexposed with respect to the European Food Safety Authority (EFSA) Tolerable Weekly Intake (TWI) for MeHg of 1.3μg/kg bw, with a mean of 0.92μg/kg bw. In the vulnerable group aged 19-45years, 29.6% exceeded the TWI. Rather than the amount of seafood consumed, the seafood choice appears to be the main determinant of the MeHg intake. Risk awareness was reported in the 49% of SFQs. Uncertainties related to such estimates from questionnaires are discussed, in order to give adequate health recommendations without compromising seafood consumption in the Mediterranean region.
Elena Dellatte; Gianfranco Brambilla; Roberto Miniero; Maria Cesarina Abete; Roberta Orletti; Giannina Chessa; Alessandro Ubaldi; Eugenio Chiaravalle; Micaela Tiso; Angelo Ferrari. Individual methylmercury intake estimates from local seafood of the Mediterranean Sea, in Italy. Regulatory Toxicology and Pharmacology 2014, 69, 105 -112.
AMA StyleElena Dellatte, Gianfranco Brambilla, Roberto Miniero, Maria Cesarina Abete, Roberta Orletti, Giannina Chessa, Alessandro Ubaldi, Eugenio Chiaravalle, Micaela Tiso, Angelo Ferrari. Individual methylmercury intake estimates from local seafood of the Mediterranean Sea, in Italy. Regulatory Toxicology and Pharmacology. 2014; 69 (1):105-112.
Chicago/Turabian StyleElena Dellatte; Gianfranco Brambilla; Roberto Miniero; Maria Cesarina Abete; Roberta Orletti; Giannina Chessa; Alessandro Ubaldi; Eugenio Chiaravalle; Micaela Tiso; Angelo Ferrari. 2014. "Individual methylmercury intake estimates from local seafood of the Mediterranean Sea, in Italy." Regulatory Toxicology and Pharmacology 69, no. 1: 105-112.
Soils contaminated with radionuclide pose a long-term radiation hazard to human health through exposure via the food chain and other pathways. Remediation of contaminated soils has become increasingly important. The feasibility to move radionuclides from superficial soil layers to a deeper level using an acid leaching solution was already illustrated previously. As this chemical treatment may influence soil equilibrium, its potential effect on germination and on the development of herbaceous plants was studied. Despite an initial delay in growth of treated plants, the same biomass production in the treated samples is reached after about one month since sowing. Even if germination is damaged by the chemical solution in a first short period, this effect is transitory. Therefore, the treatment based on this solution does not reduce the bioavailability in the uppermost soil horizon and does not compromise the yield of pastures land.
Pietro Luigi Cazzola; Agostino Cena; Stefania Squadrone; Maria Cesarina Abete; Giuseppe Ru; Francesco Ingravalle. Can chemical treatments to displace radioisotopes influence soil fertility? A laboratory trial. International Journal of Agricultural Resources, Governance and Ecology 2014, 10, 362 .
AMA StylePietro Luigi Cazzola, Agostino Cena, Stefania Squadrone, Maria Cesarina Abete, Giuseppe Ru, Francesco Ingravalle. Can chemical treatments to displace radioisotopes influence soil fertility? A laboratory trial. International Journal of Agricultural Resources, Governance and Ecology. 2014; 10 (4):362.
Chicago/Turabian StylePietro Luigi Cazzola; Agostino Cena; Stefania Squadrone; Maria Cesarina Abete; Giuseppe Ru; Francesco Ingravalle. 2014. "Can chemical treatments to displace radioisotopes influence soil fertility? A laboratory trial." International Journal of Agricultural Resources, Governance and Ecology 10, no. 4: 362.
P. Brizio; A. Benedetto; S. Squadrone; R. Tarasco; S. Gavinelli; M. Pellegrino; M. C. Abete. Heavy metals occurrence in Italian food supplements. E3S Web of Conferences 2013, 1, 15006 .
AMA StyleP. Brizio, A. Benedetto, S. Squadrone, R. Tarasco, S. Gavinelli, M. Pellegrino, M. C. Abete. Heavy metals occurrence in Italian food supplements. E3S Web of Conferences. 2013; 1 ():15006.
Chicago/Turabian StyleP. Brizio; A. Benedetto; S. Squadrone; R. Tarasco; S. Gavinelli; M. Pellegrino; M. C. Abete. 2013. "Heavy metals occurrence in Italian food supplements." E3S Web of Conferences 1, no. : 15006.
Lifting of the ban on the use of processed animal proteins (PAPs) from non-ruminants in non-ruminant feed is in the wind, avoiding intraspecies recycling. Discrimination of species will be performed through polymerase chain reaction (PCR), which is at a moment a merely qualitative method. Nevertheless, quantification of PAPs in feed is needed. The aim of this study was to approach the quantitative determination of PAPs in feed through Real Time (RT)-PCR technique; three different protocols picked up from the literature were tested. Three different kind of matrices were examined: pure animal meals (bovine, chicken and pork); one feed sample certified by the European reference laboratory on animal proteins (EURL AP) in feed spiked with 0.1% bovine meal; and genomic DNAs from bovine, chicken and pork muscles. The limit of detection (LOD) of the three protocols was set up. All the results obtained from the three protocols considered failed in the quantification process, most likely due to the uncertain copy numbers of the analytical targets chosen. This preliminary study will allow us to address further investigations, with the purpose of developing a RT-PCR quantitative method.
Daniela Marchis; Alessandro Benedetto; Giuseppina Amato; Beatrice Brusa; Stefania Squadrone; Maria Cesarina Abete. A quantitative real time polymerase chain reaction approach for estimating processed animal proteins in feed: preliminary data. Italian Journal of Food Safety 2013, 2, e4 .
AMA StyleDaniela Marchis, Alessandro Benedetto, Giuseppina Amato, Beatrice Brusa, Stefania Squadrone, Maria Cesarina Abete. A quantitative real time polymerase chain reaction approach for estimating processed animal proteins in feed: preliminary data. Italian Journal of Food Safety. 2013; 2 (1):e4.
Chicago/Turabian StyleDaniela Marchis; Alessandro Benedetto; Giuseppina Amato; Beatrice Brusa; Stefania Squadrone; Maria Cesarina Abete. 2013. "A quantitative real time polymerase chain reaction approach for estimating processed animal proteins in feed: preliminary data." Italian Journal of Food Safety 2, no. 1: e4.
Mercury (Hg) settlements in the Mediterranean Sea determine a potential toxicological relevance of seafood intakes for coastal populations. To assess this possibility, fish, molluscs, and crustaceans of commercial size of 69 different species were sampled and analyzed for total mercury (HgTOT) from georeferenced areas and evaluated for their compliance with the European Union Maximum Residue Limits of 0.5 and 1.0 mg/kg wet weight (ww). Accounting for the weekly estimated seafood intake in the Italian coastal population (mean 469–626 g/person/week) it was then possible to recover threshold contamination values in seafood. Under a Tolerable Weekly Intake of 1.3 lg/kg/bw/week, a threshold seafood contamination <0.10 mg/kg ww has been derived for sensitive groups. A suitable algorithm based on the parallel MeHg and HgTOT analysis on the most consumed species, helped to refine the uncertainties related to the conservative assumption in seafood all the HgTOT present is in form of MeHg. This work aims to improve the link between the risk management and risk assessment strategies, with the identification of those fish and seafood species, that, when regularly consumed, could determine or prevent potential HgTOT/MeHg overintakes in sensitive groups
Gianfranco Brambilla; Maria Cesarina Abete; Giovanni Binato; Eugenio Chiaravalle; Maurizio Cossu; Elena Dellatte; Roberto Miniero; Roberta Orletti; Pierluigi Piras; Alessandra Roncarati; Alessandro Ubaldi; Giannina Chessa. Mercury occurrence in Italian seafood from the Mediterranean Sea and possible intake scenarios of the Italian coastal population. Regulatory Toxicology and Pharmacology 2013, 65, 269 -277.
AMA StyleGianfranco Brambilla, Maria Cesarina Abete, Giovanni Binato, Eugenio Chiaravalle, Maurizio Cossu, Elena Dellatte, Roberto Miniero, Roberta Orletti, Pierluigi Piras, Alessandra Roncarati, Alessandro Ubaldi, Giannina Chessa. Mercury occurrence in Italian seafood from the Mediterranean Sea and possible intake scenarios of the Italian coastal population. Regulatory Toxicology and Pharmacology. 2013; 65 (2):269-277.
Chicago/Turabian StyleGianfranco Brambilla; Maria Cesarina Abete; Giovanni Binato; Eugenio Chiaravalle; Maurizio Cossu; Elena Dellatte; Roberto Miniero; Roberta Orletti; Pierluigi Piras; Alessandra Roncarati; Alessandro Ubaldi; Giannina Chessa. 2013. "Mercury occurrence in Italian seafood from the Mediterranean Sea and possible intake scenarios of the Italian coastal population." Regulatory Toxicology and Pharmacology 65, no. 2: 269-277.