Aims: This paper aimed to analyze the internal structure of the Spanish version of the Ryff’s psychological wellbeing scale (PWBS-E) among college students (n = 1700, 54.75% females, Mage = 19.23) who attend a public institution located in Veracruz, Mexico. Method: The internal structure of the PWBS-E was assessed using the confirmatory factor analysis (CFA), exploratory structural equation modeling (ESEM) and exploratory factor analysis (EFA), while the reliability of the construct was estimated with the ù coefficient and the reliability of the scores, with the α coefficient. Results: suggested that a short and unidimensional structure (19 items) provides greater empirical support. In addition, a method factor associated with inverted items in multidimensional models was evidenced. Regarding the reliability, acceptable indicators were found in both levels (construct and scores). Conclusions: This paper addresses both the findings and practical implications of the short version of the PWBS-E among college students. Objetivos: realizar el análisis de la estructura interna de la versión en español de la Escala de Bienestar Psicológico de Ryff (PWBS-E) en estudiantes universitarios (n = 1700; 54.75% mujeres; Medad = 19.23) de una institución pública en Veracruz, México. Método: La estructura interna de la PWBS-E fue evaluada mediante el análisis factorial confirmatorio (CFA), el modelamiento de ecuaciones estructurales exploratorio (ESEM) y el análisis factorial exploratorio (EFA). La confiabilidad del constructo fue estimada con el coeficiente α y de las puntuaciones con el coeficiente α. Resultados: indicaron que una estructura unidimensional y breve (19 ítems) posee mayor respaldo empírico. Además, se evidenció la existencia de un factor de método asociado a ítems invertidos en los modelos multidimensionales. En cuanto a la confiabilidad, se hallaron indicadores aceptables en ambos niveles (constructo y puntuaciones). Conclusiones: se discuten los hallazgos y las implicancias prácticas de la versión breve de la PWBS-E en universitarios. Objetivos: realizar a análise da estrutura interna de a versão em espanhol da escala de bem-estar Ryff Psychological (PWBS-E) em estudantes estudantes universitários (n = 1700; 54,75% mulheres; Medad = 19,23) de uma instituição pública em Veracruz, México. Método: A estrutura interna do PWBS-E foi avaliada por análise fatorial confirmatória (CFA), a modelagem de equações estruturais exploratórias (ESEM) e análise fatorial exploratória (EFA). O a confiabilidade da construção foi estimada com o coeficiente α e as pontuações com o coeficiente α. Resultados: indicou que uma estrutura Unidimensional e breve (19 itens) tem maior apoio empírico Além disso, a existência de um fator foi evidenciada do método associado aos itens investidos nos modelos multidimensional Quanto à confiabilidade, é eles encontraram indicadores aceitáveis ​​nos dois níveis (construto e pontuações). Conclusões: são discutidas os resultados e implicações práticas da versão resumo do PWBS-E em estudantes universitários.

This study aimed to determine the body composition profile of candidates applying for a Physical Education and Sports major. 327 young adults (F: 87, M: 240) participated in this cross-sectional study. Nutritional status and body composition analysis were performed, and the profiles were generated using an unsupervised machine learning algorithm. Body mass index (BMI), percentage of fat mass (%FM), percentage of muscle mass (%MM), metabolic age (MA), basal metabolic rate (BMR), and visceral fat level (VFL) were used as input variables. BMI values were normal-weight although VFL was significantly higher in men (<0.001; η² = 0.104). MA was positively correlated with BMR (0.81 [0.77, 0.85]; p < 0.01), BMI (0.87 [0.84, 0.90]; p < 0.01), and VFL (0.77 [0.72, 0.81]; p < 0.01). The hierarchical clustering analysis revealed two significantly different age-independent profiles: Cluster 1 (n = 265), applicants of both sexes that were shorter, lighter, with lower adiposity and higher lean mass; and, Cluster 2 (n = 62), a group of overweight male applicants with higher VFL, taller, with lower %MM and estimated energy expended at rest. We identified two profiles that might help universities, counselors and teachers/lecturers to identify applicants in which is necessary to increase physical activity levels and improve dietary habits.

Mexico has a wide variety of edible plants, such as mallow and garambullo flowers. Their composition is based on nutrients (carbohydrates, proteins, lipids, vitamins, minerals), as well as phytochemicals, organic or inorganic acids, etc. For the traditional consumption of these plants, they are boiled and then combined with other ingredients. However, after a thermal treatment, their nutritional and sensory qualities are affected. In this work, we study the effect of thermal treatment on the total phenolic compounds (TPC), antioxidant capacity, and vitamin C of two edible plants from Mexico. Both plants had a high amount of TPC as ferulic acid, p-coumaric acid, caffeic acid, quercetin, and apigenin. Both plants had a high amount of TPC as ferulic acid, p-coumaric acid, caffeic acid, quercetin, and apigenin. After thermal treatment, the samples showed a high antioxidant capacity analyzed by 2,2-Diphenyl-1-picrylhydrazyl (DPPH•), 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS●+), and ferric reducing antioxidant power (FRAP), associated with a high retention (78 and 89%) of phenolic compounds in the food matrix. Coumarin and ferulic acid were identified in both samples, while apigenin was only found in the mallow leaves and quercetin in the garambullo flowers after thermal treatment. Vitamin C content was higher on mallow leaves compared to garambullo flowers after thermal treatment. Further research is needed to characterize and evaluate the effect in both plants.

Randia aculeata (Rubiaceae), commonly known as “crucetillo,” is widely used in Mexican folk medicine, particularly its seeds, yet systematic toxicological evaluations remain scarce. This study assessed the safety of the hydroethanolic seed extract (EHRA) in Wistar rats through acute and subacute oral toxicity assays. In the acute model, a single oral dose of 2000 mg/kg produced no mortality (LD₅₀ > 5000 mg/kg), but increases in hepatic enzyme activity, reduced erythrocyte and hemoglobin levels, and focal hepatocellular necrosis suggested liver injury. In the 28-day repeated-dose study, animals receiving 250, 500, or 1000 mg/kg/day displayed progressive alterations in biochemical and hematological parameters, including elevated AST, ALT, ALP, bilirubin, urea, and creatinine, along with reduced red blood cell counts and hemoglobin levels. Histopathological analyses confirmed hepatocellular necrosis and acute tubular necrosis in the kidneys. Collectively, these findings indicate that while EHRA exhibits low acute lethality, repeated administration induces significant hepatic and renal toxicity. Considering the widespread traditional consumption of R. aculeata seeds, these results underscore the need for careful dosage considerations, evidence-based safety guidelines, and further mechanistic studies before the potential therapeutic application of this plant can be recommended.

Ensuring blood group compatibility is essential in canine transfusion medicine, and Dog Erythrocyte Antigen (DEA) 1 is the most clinically relevant erythrocyte antigen due to its immunogenicity. However, information on its prevalence in Mexico is scarce. This study assessed the distribution of DEA 1 in client-owned dogs from Veracruz–Boca del Río, southeastern Mexico, and applied antigen frequencies to generate theoretical incompatibility scenarios in untyped populations. A cross-sectional study was conducted using blood samples from 192 clinically healthy dogs. DEA 1 typing was performed using a commercial immunochromatographicassay. Frequencies were compared using chi-square or Fisher’s exact test, and 95% confidence intervals were calculated with the Wilson method. Of all dogs sampled, 60.9% (95% CI: 53.6–67.8) were DEA 1-positive. No statistically significant differences were observed by sex or municipality (p > 0.05). Although frequencies varied among breeds, no significant association between breed and DEA 1 status was detected (p > 0.05). Based on observed antigen proportions, mathematical estimations were calculated to illustrate how DEA 1 distribution alone may contribute to potential donor–recipient mismatching when typing is not performed. These estimations are theoretical values and do not represent clinically documented sensitization or transfusion reactions. This study provides the first report of DEA 1 distribution in southeastern Mexico and establishes baseline information that may support future research on canine transfusion practices and donor selection.

Effective reprocessing of surgical instruments is essential to prevent healthcare-associated infections in the field of veterinary medicine. However, chemical disinfectants are frequently used as alternatives to sterilization in small animal clinics, particularly in resource-limited settings. This preliminary exploratory study evaluated routine chemical disinfection practices and residual bacterial contamination of surgical instruments in veterinary clinics in Veracruz, Mexico. A cross-sectional study was conducted in ten small animal veterinary clinics. Samples were collected from the surgical instruments and operative surfaces immediately after routine chemical disinfection. Bacterial isolation was performed using conventional culture methods, and microbial identification was performed using MALDI-TOF mass spectrometry. Descriptive analysis and Fisher’s exact test were used to explore the association between disinfectant category and bacterial contamination. Bacterial growth was detected in 19 of the 60 samples (31.6%). The identified microorganisms included Bacillus, Staphylococcus, Pseudomonas, Acinetobacter, and Burkholderia species. Most clinics relied on low-level disinfectants, particularly benzalkonium chloride (BAC). Residual contamination was more commonly observed in clinics reporting the use of low-level disinfectants, particularly benzalkonium chloride-based products. However, these findings should be interpreted cautiously because of the exploratory observational design and limited sample size. These results suggest that routine chemical disinfection, particularly when low-level disinfectants are used, may not always achieve complete microbial elimination under real-world clinical conditions. Improved infection prevention and instrument reprocessing practices are required in veterinary clinical settings. From a One Health perspective, strengthening infection control measures in veterinary clinics may help reduce microbial circulation among animals, veterinary personnel, and the environment.