Flour from old varieties are usually considered very weak flours, and thus difficult to use in breadmaking especially when processed as Italian “Tipo 2” flour. Hence, the aim of our study was to understand if agronomic treatments can be used to improve flour processability and the quality of three old wheat varieties. An experimental strip-plot scheme was used: three old wheat varieties (Andriolo, Sieve, Verna), two seeding densities, three levels of nitrogen fertilization (N35, N80, and N135), and two levels of foliar sulfur fertilization. Analyzed parameters related to kernel composition, dough rheology and bread quality. Sulfur and nitrogen treatments significantly affected protein composition and dough alveograph strength, which increased by about 34% with nitrogen fertilization, and by about 14% with the sulfur treatment. However, only nitrogen fertilization affected bread characteristics. Crumb density significantly decreased from N35 to N135, while springiness and cohesiveness increased. On the other hand, sulfur did not improve breads. This highlight the importance of performing breadmaking tests in addition to the rheological determinations. The poor technological performance of old wheat flours can be improved with agronomical treatments designed to obtain higher-quality bread.

A research study was conducted in an open field tomato crop in order to: (i) Evaluate the capability of Sentinel-2 imagery to assess tomato canopy growth and its crop water requirements; and (ii) explore the possibility to predict crop water requirements by assimilating the canopy cover estimated by Sentinel-2 imagery into AquaCrop model. The pilot area was in Campania, a region in the south west of Italy, characterized by a typical Mediterranean climate, where field campaigns were conducted in seasons 2017 and 2018 on processing tomato. Crop water use and irrigation requirement were estimated by means of three different methods: (i) The AquaCrop model; (ii) an irrigation advisory service based on Sentinel-2 imagery known as IRRISAT and (iii) assimilating the canopy cover estimated by Sentinel-2 imagery into AquaCrop model Sentinel-2 imagery proved to be effective for monitoring canopy growth and for predicting irrigation water requirements during mid-season stage of the crop, when the canopy is fully developed. Conversely, the integration of the Sentinel-2 imagery with a crop growth model can contribute to improve the irrigation water requirement predictions in the early and development stage of the crop, when the soil evaporation is not negligible with respect to the total evapotranspiration.

The taxonomic assemblage and functions of the plant bacterial community are strongly influenced by soil and host plant genotype. Crop breeding, especially after the massive use of nitrogen fertilizers which led to varieties responding better to nitrogen fertilization, has implicitly modified the ability of the plant root to recruit an effective bacterial community. Among the priorities for harnessing the plant bacterial community, plant genotype-by-microbiome interactions are stirring attention. Here, we analyzed the effect of plant variety and fertilization on the rhizosphere bacterial community. In particular, we clarified the presence in the bacterial community of a varietal effect of N and P fertilization treatment. 16S rRNA gene amplicon sequence analysis of rhizospheric soil, collected from four wheat varieties grown under four N-P fertilization regimes, and quantification of functional bacterial genes involved in the nitrogen cycle (nifH; amoA; nirK and nosZ) were performed. Results showed that variety played the most important role and that treatments did not affect either bacterial community diversity or bacterial phyla abundance. Variety-specific response of rhizosphere bacterial community was detected, both in relation to taxa (Nitrospira) and metabolic functions. In particular, the changes related to amino acid and aerobic metabolism and abundance of genes involved in the nitrogen cycle (amoA and nosZ), suggested that plant variety may lead to functional changes in the cycling of the plant-assimilable nitrogen.

Numerous epidemiological studies have highlighted the positive effects on health of wholegrain bakery products made from ‘old’ common wheat (Triticum aestivum L.) varieties. However, ‘old’ common wheat varieties display poor rheological properties, and there is limited information on its free asparagine (ASN) content, the main precursor to acrylamide during the baking process. This paper evaluates the effects of two seeding density levels (SD: 200 and 350 seed m⁻²), three nitrogen levels (NL: 35, 80 and 135 kg N ha⁻¹), and two sulfur levels (SL: 0 and 6.4 kg S ha⁻¹) towards improving the grain yield (GY), rheological characteristics, and ASN content of 14 ‘old’ common wheat varieties. SL and SD treatments significantly increased GY without decreasing the protein content (PC), while NL significantly increased the PC without affecting GY. The dough strength (W) increased significantly with increasing SL and NL but was significantly reduced with increasing SD. ASN significantly increased by 111% as NL increased from 35 to 135 kg ha⁻¹, while ASN significantly decreased by 85.1% with the SL treatment. The findings show that 135 kg N ha⁻¹ combined with 6.4 kg S ha⁻¹ can improve the technical performance of ‘old’ wheat wholegrain flours while maintaining the ASN as low as possible.

A preliminary study was conducted to analyze the sustainability of barley production through: (i) investigating sensor-based nitrogen (N) application on barley performance, compared with conventional N management (CT); (ii) assessing the potential of the Normalized Difference Vegetation Index (NDVI) at different growth stages for within-season predictions of crop parameters; and (iii) evaluating sensor-based fertilization benefits in the form of greenhouse gasses mitigation. Barley was grown under CT, sensor-based management (RF) and with no N fertilization (Control). NDVI measurements and RF fertilization were performed using a GreenSeeker™ 505 hand-held optical sensor. Gas emissions were measured using a static chamber method with a portable gas analyzer. Results showed that barley yield was not statistically different under RF and CF, while they both differed significantly from Control. Highly significant positive correlations were observed between NDVI and production parameters at harvesting from the middle of stem elongation to the medium milk stage across treatments. Our findings suggest that RF is able to decrease CO₂ emission in comparison with CF. The relationship between N fertilization and CH₄ emission showed high variability. These preliminary results provide an indication of the benefits achieved using a simple proximal sensing methodology to support N fertilization.